PCR (Polymerase Chain Reaction) solution detection plate
By employing an inner fixing ring and pressure fasteners on the PCR solution detection plate, the problem of unstable connection between the tube and the plate was solved, achieving a stable connection and improving the accuracy and reliability of the detection.
Patent Information
- Application Number
- CN202423074721.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-13
- Publication Date
- 2025-11-21
- Estimated Expiration
- 2034-12-13
AI Technical Summary
The connection between the tube and the plate in existing PCR solution detection plates is unstable, making them prone to tilting and displacement when handled, which affects the accuracy and reliability of the detection.
Employing a unique pressure fastener design, including an inner retaining ring and pressure fasteners, a stable connection between the tube and the plate is achieved through the squeezing action of the inward-curving part and the arc-shaped inner pressure part.
This improves the connection stability of the PCR solution detection plate, avoids tilting and displacement of the tubes during handling, and enhances the accuracy and reliability of the detection.
Smart Images
Figure CN223576465U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to PCR solution detection related technical field, especially PCR solution detection board. BACKGROUND
[0002] In the field of modern medicine and biotechnology, polymerase chain reaction (PCR) technology has become a revolutionary tool, especially in viral detection, with its high sensitivity, high specificity and rapidity, it has become an important means for diagnosing viral diseases. PCR technology mainly through the "replication" of DNA molecules, makes it number rapidly increases (amplification), which can be used for subsequent detection analysis. It simulates the process of DNA replication in cells, using a special enzyme - DNA polymerase, and two specific short DNA fragments (primers), the target DNA region is rapidly amplified.
[0003] PCR solution detection is a key link in the application of PCR technology. It involves collecting samples (such as blood, urine, saliva, feces, etc.) from suspected infected persons, and processing these samples to extract viral nucleic acid. Then, by designing specific primers and using PCR amplification technology, the extracted viral nucleic acid is detected. PCR solution detection plate is a kind of in polymerase chain reaction (PCR) mainly as the primer, TaqDNA polymerase, dNTP, template nucleic acid, Mg, buffer solution, etc. The carrier of participating in amplification reaction. In order to cooperate with the gun, PCR instrument and other high throughput operation, the commonly used is 96 well or 384 well PCR plate.
[0004] Because the plate body and the pipe body of the non-integral PCR solution detection plate are separated, the pipe body can be directly inserted into the groove of the plate body, so the connection stability of the pipe body and the plate body is also important. However, at present, the pipe body is directly inserted into the groove of the plate body without stable connection structure, so that when the PCR solution detection plate is tilted, the pipe body is easy to tilt and displace relative to the plate body. To solve this problem, we propose a PCR solution detection plate. UTILITY MODEL CONTENT
[0005] An object of the utility model is to solve at least the above problems, and provide at least the advantages to be explained later.
[0006] Another object of the utility model is to provide a PCR solution detection plate, which realizes the stable connection effect of the plate body and the pipe body of the PCR solution detection plate, thereby effectively avoiding the problem that the pipe body is easy to tilt and displace relative to the plate body when the PCR solution detection plate is tilted.
[0007] To achieve the above object and some other objects, the utility model adopts the following technical scheme:
[0008] A PCR solution detection plate, comprising a detection plate body, the detection plate body is composed of a plurality of PCR sub-plates, the PCR sub-plate comprises a sub-plate body;
[0009] A PCR placing groove is arranged on the surface of the sub-plate body, and an inner fixing ring is embedded in the PCR placing groove;
[0010] A pressure fastener is arranged in the inner fixing ring, the pressure fastener comprises a fixing part, two fixing parts are fixed to the upper and lower ends of the inner surface of the inner fixing ring, the other end of the fixing part is integrally connected with an inner collecting part, the inner collecting part extends towards the center of the inner fixing ring; an arc-shaped inner pressing part is integrally connected between the two inner collecting parts, the inner surface of the arc-shaped inner pressing part is closer to the center axis of the inner fixing ring than the inner surface of the inner collecting part, the outer side of the connection between the arc-shaped inner pressing part and the inner collecting part is integrally connected with a connecting leg, the outer end of the connecting leg is integrally connected with a connecting foot, and the connecting foot is attached to the inner surface of the inner fixing ring.
[0011] Preferably, the PCR sub-plate can be longitudinally divided into a plurality of PCR sub-plates on the detection plate body, and a longitudinal mark is arranged on the upper end surface of each column of PCR sub-plates along the rear end of the last end PCR placing groove.
[0012] Preferably, the PCR sub-plate can be transversely divided into a plurality of transverse PCR sub-plates on the detection plate body, and a transverse mark is arranged on the upper end surface of each row of PCR sub-plates along the left side of the leftmost side PCR placing groove.
[0013] Preferably, upper and lower grooves are arranged on the upper and lower end surfaces of the detection plate body between adjacent PCR sub-plates.
[0014] Preferably, an outer fixed skirt is integrally connected to the lower end of the periphery of the detection plate body, outer through grooves and triangular through grooves are arranged on the outer fixed skirt between adjacent PCR sub-plates, and the outer through grooves are located at the upper end of the triangular through grooves.
[0015] Preferably, the outer fixed skirt is separated by the outer through grooves and the triangular through grooves to form an outer support edge, and the outer support edge is integrally connected to the two ends of the sub-plate body.
[0016] Preferably, the inner fixing ring comprises an outer limiting ring body and an inner ring body, the inner ring body is integrally connected to the inner side between the two outer limiting ring bodies, an embedding groove is formed between the outer limiting ring body and the inner ring body, and the inner fixing ring is clamped into the PCR placing groove through the embedding groove.
[0017] Preferably, a taking notch is arranged on the front and rear ends of the upper end surface of the outer limiting ring body, and the taking notch penetrates the outer limiting ring body.
[0018] The utility model discloses at least the following beneficial effects:
[0019] 1. In the utility model, through adopting the unique pressure fastener design, the outer surface of the pipe body gradually has the pressure effect to the upper end inner retraction portion when the pipe body inserts downwards, the inward arching range of the arc inner pressure portion increases, with the continuous downward of the pipe body, the outer surface of the pipe body gradually contacts the arc inner pressure portion and has the inward pressure to the arc inner pressure portion, the two connecting legs expand outward, the connecting foot moves along the inner ring body to the outer end, until the connecting foot contacts the inner end surface of the fixed portion, the connecting leg and the connecting foot cannot expand, and will extrude and deform outward in the space, so that the inner retraction portion further compresses the pipe body, the effect that the plate body of PCR solution detection board is connected stably with the pipe body is realized, so as to effectively avoid the problem that the pipe body is easy to incline and displace relative to the plate body when the PCR solution detection board is taken and inclined, thereby improving the accuracy and reliability of detection.
[0020] 2. In the utility model, modular design is adopted, clear horizontal and longitudinal marks are arranged on the detection board, and the notched groove and the through groove convenient to split are arranged, so that the operator can easily identify and position in the operation process, can be flexibly split according to actual demand, convenient to use, greatly reduce the operation difficulty and error rate, and greatly improve the detection efficiency.
[0021] Other advantages, objects and features of the utility model will be embodied partly through the following description, and will be understood by those skilled in the art through research and practice of the utility model. BRIEF DESCRIPTION OF DRAWINGS
[0022] Figure 1 It is the overall structure schematic diagram of the upper view angle of the PCR solution detection board of the utility model;
[0023] Figure 2 It is the overall structure schematic diagram of the lower view angle of the PCR solution detection board of the utility model;
[0024] Figure 3 It is the plan view of the PCR solution detection board of the utility model;
[0025] Figure 4 It is the section view at A-A of the PCR solution detection board of the utility model;
[0026] Figure 5 It is the structure enlarged view at B of the PCR solution detection board of the utility model;
[0027] Figure 6 It is the connection structure schematic diagram of the inner fixing ring and the pressure fastener of the PCR solution detection board of the utility model;
[0028] Figure 7The utility model discloses a PCR solution detection plate's C place structure enlargement view. DETAILED DESCRIPTION
[0029] The utility model discloses a PCR solution detection plate's C place structure enlargement view.
[0030] As Figures 1-7 PCR solution detection plate, including detection board body 1, the detection board body 1 is formed by a plurality of PCR sub-plate 9, and the PCR sub-plate 9 includes sub-plate body 10;
[0031] PCR placement groove 12, its array is opened in the sub-plate body 10 board face, and the PCR placement groove 12 is embedded with inner fixed ring 13;
[0032] Pressure fastener 18, annular array in the inner fixed ring 13 inside, the pressure fastener 18 includes fixed part 19, and two fixed part 19 are fixed to the upper and lower ends of inner fixed ring 13 inner surface, and the other end of fixed part 19 is integrally connected with inner retraction part 20, and inner retraction part 20 expands towards the inner center of inner fixed ring 13;Two the inner retraction part 20 is integrally connected with arc-shaped inner pressure part 21, and the inner surface of arc-shaped inner pressure part 21 is spaced apart from the center axis of inner fixed ring 13 than the inner surface of inner retraction part 20 and the center axis of inner fixed ring 13, and the outer side of the connecting portion of arc-shaped inner pressure part 21 and inner retraction part 20 is integrally connected with connecting leg 22, and the outer end of connecting leg 22 is integrally connected with connecting foot 23, and connecting foot 23 is attached to the inner surface of inner fixed ring 13.
[0033] In the above scheme, the inner fixed ring is used for fixing the pipe body;The pipe body is inserted into the inner fixed ring downwards, and as the pipe body continues to go down, the outer surface of the pipe body gradually has pressure effect on the upper end inner retraction part, so that the arch-shaped inner pressure part increases the arching amplitude inward;As the pipe body continues to go down, the outer surface of the pipe body gradually contacts the arch-shaped inner pressure part and has inward pressure on the arch-shaped inner pressure part, so that the two connecting legs expand outward, the connecting foot moves along the inner ring body to the outer end, until the connecting foot contacts the inner end surface of the fixed part, the connecting leg and the connecting foot cannot expand, and will be extruded outward in the space, so that the inner retraction part further compresses the pipe body;That is, the inner retraction part and the arch-shaped inner pressure part have extrusion effect on the pipe body to fix the pipe body.
[0034] The design of the inner fixed ring makes the pipe body be extruded by the inner retraction part and the arch-shaped inner pressure part when the pipe body is inserted into the inner fixed ring, so that stable fixation is realized. This unique pressure fastener design makes the pipe body not easy to fall off during the detection process, greatly improves the accuracy and reliability of the detection.
[0035] In one preferred embodiment, the PCR sub-plates 9 are longitudinally divided into a plurality of PCR sub-plates 9 on the detection plate plate body 1, and each column of the PCR sub-plates 9 is provided with a longitudinal mark 4 on the upper end surface along the rear end of the last end PCR placement slot 12.
[0036] In the above embodiment, the PCR sub-plates can be longitudinally divided into a plurality of longitudinal PCR sub-plates according to requirements, and the longitudinal marks make it easy for the operator to find the required PCR placement slot.
[0037] In one preferred embodiment, the PCR sub-plates 9 are longitudinally divided into a plurality of PCR sub-plates 9 on the detection plate plate body 1, and each column of the PCR sub-plates 9 is provided with a longitudinal mark 4 on the upper end surface along the rear end of the last end PCR placement slot 12.
[0038] In the above embodiment, the PCR sub-plates can be longitudinally divided into a plurality of longitudinal PCR sub-plates according to requirements, and the longitudinal marks make it easy for the operator to find the required PCR placement slot.
[0039] In one preferred embodiment, the detection plate plate body 1 is provided with upper and lower grooves 5 and 6 on the upper and lower end surfaces between adjacent PCR sub-plates 9.
[0040] In the above embodiment, the grooves separate the PCR sub-plates in the longitudinal and transverse directions.
[0041] In one preferred embodiment, the detection plate plate body 1 is integrally connected with an outer fixed skirt 2 at the lower end of the periphery, and the outer fixed skirt 2 is provided with an outer through slot 7 and a triangular through slot 8 between adjacent PCR sub-plates 9, and the outer through slot 7 is located at the upper end of the triangular through slot 8.
[0042] In the above embodiment, the outer through slot and the triangular through slot on the outer fixed skirt make it easy for the operator to tear and separate the PCR sub-plates. When no hole is needed, the detection plate plate body can be torn. The outer support edges on both sides of the tearing position are taken and forced in two directions, so that the two plate bodies are separated along the upper and lower grooves; and the operation is repeated to complete the separation of the transverse plate body or the longitudinal plate body.
[0043] Not only is it convenient to use, but also makes the detection plate flexible to configure according to actual requirements, greatly improving the detection efficiency.
[0044] In one preferred embodiment, the PCR sub-plates 9 separate the outer fixed skirt 2 into outer support edges 11 through the outer through slot 7 and the triangular through slot 8, and the outer support edges 11 are integrally connected to both ends of the sub-plate body 10.
[0045] In the above embodiment, after the PCR sub-plate is torn, the outer fixed skirt is separated into outer support edges, which play a supporting role.
[0046] In one preferred embodiment, the inner fixing ring 13 comprises outer limiting ring bodies 14 and an inner ring body 15, the inner ring body 15 is integrally connected to the inner side between the two outer limiting ring bodies 14, the outer limiting ring bodies 14 and the inner ring body 15 form an embedding groove 16, and the inner fixing ring 13 is clamped into the PCR placing groove 12 through the embedding groove 16.
[0047] In the above scheme, the embedding groove enables the inner fixing ring to be easily clamped into the PCR placing groove, thereby achieving stable connection. Not only does it simplify the installation process, but also improves the stability and durability of the inner fixing ring.
[0048] In one preferred embodiment, the front and rear ends of the upper end surface of the outer limiting ring body 14 are provided with taking notches 17, and the taking notches 17 penetrate through the upper end of the outer limiting ring body 14.
[0049] In the above scheme, the existence of the taking notches enables the pipe body to be more tightly inserted and conveniently pulled out.
[0050] Although the embodiments of the present application have been disclosed as above, it is not limited to the application listed in the specification and the embodiments, and it can be fully applied to various fields suitable for the present application. For those skilled in the art, other modifications can be easily realized, and therefore the present application is not limited to specific details and the figures shown and described herein.
Claims
1. A PCR solution detection plate, wherein, The system includes a detection plate body, which is composed of multiple PCR sub-plates, each of which includes a sub-plate body. PCR placement slots are arrayed on the surface of the plate body, and an inner fixing ring is embedded in the PCR placement slots. A pressure fastener is arranged in a ring array inside the inner fixing ring. The pressure fastener includes a fixing part, two fixing parts are fixed to the upper and lower ends of the inner surface of the inner fixing ring, and the other end of the fixing part is integrally connected to an inwardly tapered part that extends toward the center of the inner fixing ring. An arc-shaped inner pressure part is integrally connected between the two inwardly tapered parts. The distance between the inner surface of the arc-shaped inner pressure part and the central axis of the inner fixing ring is less than the distance between the inner surface of the inwardly tapered part and the central axis of the inner fixing ring. A connecting leg is integrally connected to the outer side of the connection between the arc-shaped inner pressure part and the inwardly tapered part. A connecting foot is integrally connected to the outer end of the connecting leg and fits against the inner surface of the inner fixing ring.
2. The PCR solution detection plate as described in claim 1, wherein, The PCR plate can be longitudinally divided into multiple PCR plates on the detection plate body, and each column of the PCR plate has a longitudinal marker on the upper surface along the rear end of the PCR placement slot.
3. The PCR solution detection plate as described in claim 1, wherein, The PCR plate can be divided into multiple horizontal PCR plates on the detection plate body, and a horizontal mark is provided on the upper surface of each row of PCR plates along the left side of the leftmost PCR placement slot.
4. The PCR solution detection plate as described in claim 1, wherein, The detection plate has an upper groove and a lower groove on its upper and lower end faces along the distance between adjacent PCR plates.
5. The PCR solution detection plate as described in claim 4, wherein, The lower periphery of the detection plate is integrally connected with an outer fixing skirt. An outer through groove and a triangular through groove are formed on the upper edge of the outer fixing skirt between the adjacent PCR plates. The outer through groove is located at the upper end of the triangular through groove.
6. The PCR solution detection plate as described in claim 5, wherein, The PCR plate is divided into an outer fixed skirt by an outer through groove and a triangular through groove to form an outer support edge, which is integrally connected to both ends of the plate body.
7. The PCR solution detection plate as described in claim 1, wherein, The inner fixation ring includes an outer limiting ring body and an inner ring body. The inner ring body is integrally connected to the inner side between the two outer limiting ring bodies. An embedding groove is formed between the outer limiting ring bodies and the inner ring body. The inner fixation ring is inserted into the PCR placement groove through the embedding groove.
8. The PCR solution detection plate as described in claim 7, wherein, The upper end face of the outer limiting ring has a pick-up notch at the front and rear ends, and the pick-up notch penetrates the upper outer limiting ring.