Cell culture filtering device

Abstract

The utility model discloses a cell culture filtering device which comprises a base, the microporous filter membrane is movably mounted in the base; the gland is arranged on the base in a sliding mode and abuts against the microfiltration membrane, and a first culture bottle is installed on the gland in a threaded mode; the fixed block is fixedly mounted at the bottom end of the base; the second culture bottle is mounted in the fixing block in a threaded manner and is propped against the base. According to the cell culture filtering device provided by the utility model, after the microfiltration membrane is fixed, the first culture bottle filled with cells is mounted on the gland in a threaded manner, and meanwhile, the clean second culture bottle is mounted on the fixed block in a threaded manner, so that the first culture bottle and the second culture bottle are respectively propped against the front surface and the back surface of the microfiltration membrane; the microfiltration membrane is arranged in the first culture bottle, so that cells in the first culture bottle penetrate through the microfiltration membrane and then enter the second culture bottle to be stored, the microfiltration membrane can be fixed, bottle openings of the first culture bottle and the second culture bottle can be prevented from loosening and dislocation, and the safety during cell filtration is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture technical field, concretely relates to a cell culture filtering device. BACKGROUND

[0002] The cell culture filtering device plays an important role in experiments, which can effectively remove impurities and microorganisms in the cell culture medium and ensure the purity of the culture environment.

[0003] The traditional cell culture filtering device adopts a microporous filter membrane for filtering. The microporous filter membrane is placed between a culture bottle containing cells and another empty culture bottle, and the two culture bottles are fixed by a clamping assembly, so that when the culture bottle containing cells is poured, the cells can flow into the empty culture bottle through the microporous filter membrane, thereby filtering out impurities and microorganisms in the cells. However, when the microporous filter membrane is installed at the connection between the two culture bottles, it is easy to cause misalignment of the bottle openings of the two culture bottles, so that the cells flow out from the connection between the two bottle openings during the filtering process. In addition, the positions of the two culture bottles need to be rotated and exchanged during the filtering of the cells. Once the movement amplitude is too large, the connection between the two culture bottles will be loose, thereby affecting the filtering effect of the microporous filter membrane, and in severe cases, it will also cause cell leakage and contamination. SUMMARY

[0004] The utility model aims at providing a cell culture filtering device to solve the above problems.

[0005] To achieve the above purpose, the utility model provides the following technical scheme: a cell culture filtering device, comprising:

[0006] a base;

[0007] a microporous filter membrane movably installed in the base;

[0008] a gland slidingly arranged on the base and abutting against the microporous filter membrane, the gland being screw-mounted with a first culture bottle;

[0009] a fixed block fixedly installed at the bottom end of the base;

[0010] a second culture bottle screw-mounted in the fixed block and abutting against the base.

[0011] As a preferred, the gland is provided with a plurality of insertion blocks arranged in a circumferential array, the side wall of the insertion block is provided with a plurality of symmetrically arranged sliding grooves, a first spring is fixedly installed in each sliding groove, and a lock catch is fixedly installed on the end face of the first spring.

[0012] As a preferred, a plurality of lock holes are arranged in a circumferential array in the base, and the lock holes are connected with the lock catches.

[0013] The side wall of the lock catch is preferably fixedly provided with a pressing block.

[0014] The base is preferably provided with a plurality of circumferential grooves, and a second spring is fixedly arranged in each groove.

[0015] The inner wall of the pressing cover is preferably provided with a threaded groove.

[0016] The inner wall of the fixing block is preferably provided with a threaded groove.

[0017] In the above technical solution, the cell culture filtering device has the following advantages:

[0018] (1) The base, the microporous filter membrane, the pressing cover, the first culture bottle, the fixing block and the second culture bottle are arranged, so that the bottle opening of the first culture bottle and the second culture bottle can be fixed on the front and back surfaces of the microporous filter membrane. The pressing cover is matched with the base, so that the microporous filter membrane is pressed and fixed. After the microporous filter membrane is fixed, the first culture bottle containing cells is screwed onto the pressing cover, and the clean second culture bottle is screwed onto the fixing block, so that the first culture bottle and the second culture bottle abut on the front and back surfaces of the microporous filter membrane, respectively. Therefore, the cells in the first culture bottle pass through the microporous filter membrane and enter the second culture bottle for storage. The microporous filter membrane is fixed, and the bottle opening of the first culture bottle and the second culture bottle is prevented from loosening and mispositioning, so that the safety of cell filtering is improved.

[0019] (2) The insertion block, the sliding groove, the first spring, the lock catch and the lock hole are arranged, so that the pressing cover can be fixed, and the pressing cover can quickly press and fix the microporous filter membrane.

[0020] (3) The pressing block is arranged, so that the lock catch can be conveniently driven.

[0021] (4) The groove and the second spring are arranged, so that the pressing cover can be separated from the base. BRIEF DESCRIPTION OF DRAWINGS

[0022] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art according to these drawings.

[0023] Figure 1 It is a schematic view of the pressing cover structure of the present application.

[0024] Figure 2 It is a schematic view of the base structure of the present application.

[0025] Figure 3 It is the cross section structure schematic view of the microporous filter membrane of the utility model;

[0026] Figure 4 It is the cross section structure schematic view of the mounting block of the utility model;

[0027] Figure 5 It is the cross section structure schematic view of the mounting block of the utility model.

[0028] Mark explanation:

[0029] 1, base;2, gland;3, fixed block;4, plug;5, sliding slot;6, first spring;7, lock catch;8, lock hole;9, recess;10, second spring;11, microporous filter membrane;12, first culture bottle;13, second culture bottle;15, dial block. Specific implementation

[0030] In order to make the technical personnel of the prior art better understand the technical scheme of the utility model, the utility model will be further introduced in detail below in conjunction with the drawings.

[0031] As Figures 1-5 Indicated, a kind of cell culture filtration device, comprising:

[0032] Base 1;

[0033] Microporous filter membrane 11 is movably installed in base 1;

[0034] Gland 2 is slidably arranged on base 1 and abuts microporous filter membrane 11, and first culture bottle 12 is screw-mounted on gland 2;

[0035] Fixed block 3 is fixedly installed at the bottom end of base 1;

[0036] Second culture bottle 13 is screw-mounted in fixed block 3 and abuts base 1.

[0037] Specifically, when cell needs to be filtered, by placing microporous filter membrane 11 in base 1, and installing gland 2 on base 1, so that gland 2 is pressed on microporous filter membrane 11, so that microporous filter membrane 11 can be fixed in base 1, when microporous filter membrane 11 is fixed, by screwing first culture bottle 12 containing cells on gland 2, so that the bottle mouth of first culture bottle 12 is fixed and abuts on microporous filter membrane 11, when first culture bottle 12 containing cells is installed, by screwing clean second culture bottle 13 to fixed block 3, so that the bottle mouth of second culture bottle 13 abuts on base 1, so that the cell liquid filtered by microporous filter membrane 11 can directly flow into second culture bottle 13, effectively prevent two culture bottles from loosening or displacement to cause cell pollution and leakage.

[0038] In the above embodiment, by arranging the base 1, the microporous filter film 11, the cover 2, the first culture bottle 12, the fixing block 3 and the second culture bottle 13, the bottle opening of the first culture bottle 12 and the second culture bottle 13 can be conveniently fixed on the front and back surfaces of the microporous filter film 11, the microporous filter film 11 is fixed by cooperating the cover 2 with the base 1, after the microporous filter film 11 is fixed, the first culture bottle 12 containing cells is screwed onto the cover 2, and the clean second culture bottle 13 is screwed onto the fixing block 3, so that the first culture bottle 12 and the second culture bottle 13 abut on the front and back surfaces of the microporous filter film 11 respectively, thereby enabling the cells in the first culture bottle 12 to pass through the microporous filter film 11 and enter the second culture bottle 13 for storage, which not only fixes the microporous filter film 11, but also avoids loosening and mispositioning of the bottle openings of the first culture bottle 12 and the second culture bottle 13, thereby improving the safety during cell filtration.

[0039] As a further provided embodiment of the utility model, the cover 2 is provided with a plurality of insertion blocks 4 arranged in a circumferential array, the side wall of the insertion block 4 is provided with a plurality of symmetrical sliding grooves 5, the first spring 6 is fixedly installed in the sliding groove 5, and the lock catch 7 is fixedly installed on the end face of the first spring 6.

[0040] Further, the base 1 is provided with a plurality of lock holes 8 arranged in a circumferential array, and the lock catch 7 is clamped in the lock hole 8.

[0041] Specifically, when the cover 2 is installed on the base 1, the cover 2 is limited by inserting the insertion block 4 into the base 1, so as to avoid rotation of the cover 2 during cell filtration, the first spring 6 installed in the sliding groove 5 pushes the lock catch 7 to be clamped in the lock hole 8, so that the cover 2 is fixed on the base 1 by cooperation of the lock catch 7 and the lock hole 8, thereby enabling the cover 2 to press and fix the microporous filter film 11 in the base 1, avoiding displacement of the microporous filter film 11, and improving the stability of the microporous film during filtration.

[0042] As another further provided embodiment of the utility model, the side wall of the lock catch 7 is fixedly installed with a poking block 15.

[0043] Further, the base 1 is provided with a plurality of recesses 9 arranged in a circumferential array, the second spring 10 is fixedly installed in the recess 9, and the top end of the second spring 10 is movably connected to the insertion block 4.

[0044] Specifically, after cell filtration is completed, the lock catch 7 is driven by the poking block 15 to be poked, so that the lock catch 7 is separated from the lock hole 8, thereby unlocking the insertion block 4, the second spring 10 installed in the recess 9 provides a pushing force to the insertion block 4, so that the insertion block 4 moves upward and is separated from the base 1, and the cover 2 is separated from the base 1, so that the microporous filter film 11 can be replaced.

[0045] As further provided by the utility model, a threaded groove is formed in the inner wall of the cover 2.

[0046] Specifically, the first culture bottle 12 is stably installed on the cover 2 through the threaded groove formed in the cover 2 and the thread on the outer wall of the first culture bottle 12, and the first culture bottle 12 can be quickly removed after use.

[0047] As further provided by the utility model, a threaded groove is formed in the inner wall of the fixed block 3.

[0048] Specifically, the second culture bottle 13 is stably installed on the fixed block 3 through the threaded groove formed in the fixed block 3 and the thread on the second culture bottle 13, and the second culture bottle 13 can be quickly removed after use.

[0049] Working principle: when the cells need to be filtered, the microporous filter membrane 11 is placed in the base 1, the plug 4 installed on the cover 2 is driven to be inserted into the base 1, so that the cover 2 is installed on the base 1 and is pressed to the microporous filter membrane 11, the lock catch 7 is pushed into the lock hole 8 through the first spring 6 installed in the plug hole, so that the cover 2 is locked on the base 1, when the cover 2 is fixed, the first culture bottle 12 is threadedly connected to the cover 2, so that the first culture bottle 12 is fixed in the cover 2 and abuts against the microporous filter membrane 11, so that the cells in the first culture bottle 12 can flow into the microporous filter membrane 11 for filtration, the second culture bottle 13 is threadedly connected to the fixed block 3, so that the second culture bottle 13 can abut against the base 1, so that the cells filtered by the microporous filter membrane 11 can flow into the second culture bottle for collection, avoiding loosening or displacement of the first culture bottle 12 and the second culture bottle 13 during cell filtration, when the cover 2 needs to be disassembled, the lock catch 7 is controlled to be disengaged from the lock hole 8 through the control knob 15, so that the cover 2 is unlocked, when the cover 2 is unlocked, the cover 2 is pushed out of the base 1 through the second spring 10, so that the cover 2 is disassembled.

[0050] The above describes certain exemplary embodiments of the utility model only by way of illustration, without doubt, for ordinary skilled in the art, under the condition of not deviating from the spirit and scope of the utility model, the described embodiments can be modified in various ways. Therefore, the above drawings and description are illustrative in nature and should not be understood as limiting the scope of protection of the utility model claims.

Claims

1. A cell culture filtration device, characterized in that, The utility model relates to a culture bottle fixing device, including: Base (1); The microporous filter membrane (11) of activity installation in base (1); The gland (2) of sliding setting on base (1) and abutting to microporous filter membrane (11), first culture bottle (12) are installed on the gland (2) screw thread, The fixed block (3) of fixed installation in base (1) bottom end; Second culture bottle (13) are installed in fixed block (3) screw thread and abutting to base (1).

2. The cell culture filtration device of claim 1, wherein, The plug-in block (4) of presenting circumferential array is provided in the gland (2), the side wall of plug-in block (4) is opened to the symmetrical slide groove (5) of setting, first spring (6) are fixedly installed in slide groove (5), the end surface of first spring (6) is fixedly installed with lock catch (7).

3. The cell culture filtration device of claim 1, wherein, The lock hole (8) of presenting circumferential array is opened in base (1), and lock catch (7) are clamped in lock hole (8).

4. The cell culture filtration device of claim 3, wherein, The side wall of lock catch (7) is fixedly installed with the knob (15).

5. The cell culture filtration device of claim 1, wherein, The recess (9) of presenting circumferential array is opened in base (1), and second spring (10) are fixedly installed in recess (9), and the top end of second spring (10) is movably connected to plug-in block (4).

6. The cell culture filtration device of claim 1, wherein, The inner wall of gland (2) is opened to the thread groove.

7. The cell culture filtration device of claim 1, wherein, The inner wall of fixed block (3) is opened to the thread groove.

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