Vibration water bath kettle for cell resuscitation

Abstract

The utility model discloses a vibration water bath kettle for cell resuscitation, which comprises a kettle body and a vibration plate, the vibration disc is vertically inserted into the pot body, a mounting hole is formed in the part, located in the pot body, of the vibration disc, and a vibration device, a power supply and a control panel are mounted on the part, located above the pot body, of the vibration disc; the control panel is respectively connected with the vibration device and the power supply; and an O-shaped sealing ring is mounted in the mounting hole. According to the utility model, cryopreserved cells in the pot body are driven to vibrate and rub along with physical vibration of the vibration device and the vibration disc, and based on the design of physical vibration, the influence of other factors on the recovery effect is avoided; the vibration motor is used for acting, high speed and high frequency of vibration of the cryopreserved cells can be achieved, the vibration expansion effect of the vibration disc is achieved, and high efficiency of vibration of the cryopreserved cells can be achieved.

Description

TECHNICAL FIELD

[0001] The utility model relates to a water bath, specifically to a vibrating water bath for cell recovery. BACKGROUND

[0002] Cell recovery refers to the rapid thawing of cells stored in liquid nitrogen, -80 DEG C or below 0 DEG C, and the recovery of cell growth technology; The traditional recovery technology is to thaw the cells in the water bath, and to shake and oscillate from time to time to speed up the thawing of the cells; The recovery process is mainly operated manually by the experimenter, and the difference between personnel has a great influence on the accuracy and precision of the recovery data; At the same time, when multiple cells are recovered at the same time or the liquid level is high, the personnel operation is easy to cause the liquid in the water bath to splash and pollute the experimental environment.

[0003] The existing water bath, for example, technical scheme 1 (CN118773007A): ultrasonic water bath, the scheme installs ultrasonic generator in the water bath, and uses ultrasonic wave to realize the rapid recovery of frozen cells, but has the following shortcomings: ultrasonic wave is often used for cleaning solid objects in the laboratory, frozen cells gradually melt into liquid when recovering, and the liquid contains fragile and dense cells, whether different frequency ultrasonic waves will have adverse effects on different types of frozen cells is not yet determined.

[0004] For example: technical scheme 2 (CN221371139U): driving motor stirring rotary water bath, the scheme installs driving motor outside the water bath, and inside the water bath, the vibration disc, threaded rod, guide rod and the like are assembled; The device drives the threaded rod to rotate forward and backward through the driving motor, the threaded rod drives the main body moving frame to move back and forth, and then drives the vibration disc to move back and forth, so that the cells in the frozen tube can be quickly heated and recovered, after the recovery is completed, the vibration disc is lifted by the electric push rod, so as to take out the frozen tube, but has the following shortcomings: a. The vibration disc moves back and forth in the pot, which is easy to form large water splashes in the pot, and the splashing of water splashes may cause pollution of the experimental environment; b. When the driving motor moves back and forth in the water bath, in order to ensure the safety of the water bath, the speed of the motor and the vibration disc will not be fast, and the cells in the frozen tube can only be thawed at a slow speed, which will damage the survival rate and state of the cells after recovery.

[0005] And as: Prior art solution 3 (CN218932160U): floating shock water bath, the scheme installs the frame in the water bath, the frame installs the detachable float, the frame is installed with the oscillation device; When using, the frame drives the float to oscillate, and then the oscillation is conducted to the freeze tube on the float, and the thawing of the freeze cells is accelerated, but there are the following shortcomings: a. Install the frame in the water bath, which complicates the internal space of the water bath, causing trouble for daily cleaning and disinfection of the water bath; B. The oscillation generating mechanism and control device installed on the frame have power supply, and the frame and the water bath liquid surface are in direct contact, which is easy to cause electric shock and short circuit during use, at the same time, the frame is in motion state during instrument operation, and the control device on the frame is not easy to touch and adjust, which increases the experimental risk. Utility model content

[0006] The utility model discloses to solve the technical problem of providing a novel water bath capable of thawing quickly, keeping cells in good condition and maintaining clean experimental environment.

[0007] To solve the above technical problems, the utility model discloses a technical solution of a vibrating water bath for cell recovery:

[0008] Including the kettle body, still including the vibration disc, the vibration disc is inserted into the kettle body vertically, and the vibration disc is provided with mounting holes in the part in the kettle body, and the vibration disc is provided with vibration device, power supply and control panel above the kettle body; The control panel is connected with the vibration device and the power supply respectively; The mounting hole is provided with an O-shaped sealing ring.

[0009] The bottom of the kettle body is provided with a clamping groove; The vibration disc is inserted into the clamping groove below.

[0010] The vibration device is a vibration motor.

[0011] Both sides of the vibration disc are connected with two left and right threaded connecting seats; The threaded connecting seat is threadedly connected with the mounting rod; The mounting rod is connected with the hanging rod; The hanging rod is threadedly connected with the limiting cap; The hanging rod is hung on the kettle body, and is limited by the limiting cap and the mounting rod.

[0012] The thickness of the vibration disc is ≤1cm, and the material is stainless steel convenient for vibration conduction.

[0013] The utility model discloses can reach technical effect is: the utility model discloses utilize the physical vibration of vibration device and vibration disc, drive the frozen cell in the pot body vibration friction, based on the design of physical vibration, avoid the influence of other factors to the recovery effect, utilize the work of vibration motor, can realize the high speed and high frequency of frozen cell vibration, also have the expansion effect of vibration disc to vibration, can realize the high efficiency of frozen cell vibration, and the utility model discloses will vibration disc and the pot body set up as detachable connection, not easy to influence the daily cleaning and disinfection of pot body, simultaneously, vibration device, power and control panel are installed on the top of vibration disc, do not contact with the liquid level in the pot body, avoid the circuit problem caused by water inlet, after the cell is immersed in the liquid level of pot body when using, can continuously operate after opening switch, and the liquid level will not produce larger fluctuation because of instrument operation, avoid environmental pollution, simultaneously, the frozen cell tube is placed in the vibration disc, and the vibration disc is fixed to the bottom of the pot body, so that the cell tube will not appear big movement, convenient for observing the thawing progress of frozen cell in the experiment, improve cell recovery effect. BRIEF DESCRIPTION OF DRAWINGS

[0014] The utility model discloses make further detailed explanation in combination with specific embodiment and drawings:

[0015] Figure 1 It is the structure schematic diagram of a kind of vibration water bath pot for cell recovery of the utility model;

[0016] Figure 2 It is Figure 1 A enlarged view of of the utility model;

[0017] Figure 3 It is the explosion drawing of a kind of vibration water bath pot for cell recovery of the utility model. DETAILED DESCRIPTION

[0018] The utility model is further detailed in combination with drawings.

[0019] Refer to Figures 1 to 3 .

[0020] A kind of vibration water bath pot for cell recovery, including pot body 1, still include vibration disc 2, specifically, the thickness of vibration disc 2 ≤1cm, material is the stainless steel material of being convenient for transmission vibration;Vibration disc 2 is inserted into in pot body 1 vertically, specifically, pot body 1 inner bottom is provided with clamping groove 7, vibration disc 2 is inserted into in clamping groove 7 below, more specifically, pot body 1 inner bottom is provided with two clamping grooves 7;Vibration disc 2 is provided with mounting hole in the portion in pot body 1, O-shaped sealing ring 3 is installed in mounting hole;Vibration disc 2 is provided with vibration device 4, power 5 and control panel 6 above the portion in pot body 1, control panel 6 is connected with vibration device 4 and power 5 respectively;Specifically, vibration device 4 is vibration motor.

[0021] The utility model discloses a pot body 1 is used for maintaining the temperature of liquid used in water bath, and plays a stabilizing role as a clamping groove base, on the basis of traditional temperature control water bath pot, 2 fixed clamping grooves 7 are left on the inside bottom of pot body 1, the clamping groove 7 is designed as the shape of the embedded vibration disc 2 plug, plays the role of fixed vibration disc 2, the vibration disc 2 is the main load-bearing component of the cell recovery and vibration related parts, the thickness of vibration disc 2 is less than or equal to 1cm, the main body adopts the stainless steel material or other suitable material convenient for conducting vibration, the vibration disc 2 is the rectangular plate material with 2 protruding fixed plugs at the bottom end, the lower half of vibration disc 2 is installed O-shaped sealing ring 3, the upper half of vibration disc 2 is installed power supply 5, vibration device 4 and control panel 6, and the components installed in the upper half have waterproof characteristics, meanwhile, for installing components, vibration disc 2 leaves a certain number and shape of mounting holes, as an optimization, power supply 5 is rechargeable power supply.

[0022] O-shaped sealing ring 3 is used for fixing the cryopreservation tube, the thickness of O-shaped sealing ring 3 needs to be greater than vibration disc 2, is embedded in the mounting hole left in the lower half of vibration disc 2, and O-shaped sealing ring 3 is stable and waterproof and heatproof after embedding, and the main function is to fix the cryopreservation tube on vibration disc 2, ensures that the cryopreservation tube does not separate when vibration disc 2 is embedded in the water bath pot and vibrates, and better transmits the vibration caused by vibration device 4 to the frozen cells in the cryopreservation tube, simultaneously, the radius and number of O-shaped sealing ring 3 can be selected, and different radius and number of O-shaped sealing ring 3 can be selected for different specifications and numbers of cryopreservation tubes to ensure that the cryopreservation tube can be fixed.

[0023] Vibration device 4 is a part for vibration, and the main part for work is a vibration motor or other similar vibration source, in order to meet the diversity of frozen cell types, relevant circuits and parts are matched to provide different vibration frequencies and intensities as options, and the vibration source and the matched parts can be waterproof and heatproof, in order to ensure that the vibration of the whole vibration disc 2 is uniform and stable, vibration device 4 is installed at the axial position of vibration disc 2.

[0024] Power supply 5 provides stable power supply for the electrical parts on the vibration vibration disc 2, because the power supply 5 is in contact with the liquid in the water bath pot more in the use process, so the power supply 5 uses waterproof and heatproof materials, and at the same time, the power supply 5 is sealed and waterproof except for the waterproof interface used for charging.

[0025] Control panel 6 is the hub of power supply 5 and vibration device 4, and the relevant functions can be connected with mobile phone APP for use, displays the power value in the power supply, and sends an alarm when the value is lower than the warning value, provides multiple vibration modes, and can set and change the relevant mode data in the vibration device, and the mode data generally includes but is not limited to power, time and speed, etc.

[0026] The clamping groove 7 is a clamping groove structure for fixing the vibration disc 2; two recessed clamping grooves 7 are arranged on the inner bottom of the traditional water bath, and correspond to the two protruding fixing plugs at the bottom end of the vibration disc; the vibration disc 2 can be vertically installed on the bottom plane of the pot, so as to ensure the stable connection of the vibration disc 2 and the pot body 1 under the maximum vibration power; at the same time, a certain elastic space is preferably provided between the clamping groove 7 and the vibration disc 2 to ensure the transmission of vibration, so that the stable effect of the clamping groove 7 will not reduce the vibration transmitted.

[0027] Working principle: the vibration caused by the vibration device 4 is transmitted to the cryopreservation tube fixed in the O-shaped sealing ring 3 through the vibration disc 2, the O-shaped sealing ring 3 and the cryopreservation tube are both located below the liquid surface of the liquid in the water bath to reach the set temperature, the transmitted vibration makes the cryopreserved cells in the cryopreservation tube vibrate, and the thawing of the cryopreserved cells is accelerated; by adjusting the related settings of the vibration device 4, the size of the vibration received by the cryopreserved cells will also change, and corresponding parameters can be set for different cryopreserved cells.

[0028] Working process: after the water bath reaches the preset temperature, corresponding vibration parameters are selected and vibration time is set on the control panel 6 or the mobile phone APP according to the type of the thawed cells and the type of the cryopreservation tube; the cryopreservation tube to be thawed is inserted into the O-shaped sealing ring 3 of the vibration disc 2 and fixed, the two protruding fixing plugs at the bottom end of the vibration disc 2 are embedded and fixed in the clamping grooves 7 at the bottom of the water bath, so as to ensure that the cryopreservation tube is below the liquid surface of the water bath; the vibration device 4 is started on the control panel 6, and the whole vibration disc 2 starts to vibrate, and the cryopreservation tube below the liquid surface also vibrates, so that the cryopreserved cells start to accelerate thawing, and the operator observes the thawing process of the cells in the cryopreservation tube through the liquid surface; after the cells are thawed, the vibration is stopped on the control panel 6 or the mobile phone APP, the cryopreservation tube is taken out from the O-shaped sealing ring 3 after the vibration disc 2 is pulled out, and the subsequent operation of cell recovery is carried out.

[0029] Compared with the prior art, the present application can achieve the following beneficial effects:

[0030] 1. The water bath vibration thawing method similar to the traditional recovery method avoids the influence of other factors on the recovery effect;

[0031] 2. The vibration device 4 can improve the vibration frequency and intensity of the cells during thawing, which can accelerate the thawing of the cells compared with the traditional manual oscillation, and the uniformity of the work of the vibration device can avoid errors caused by personnel differences during cell recovery, thereby improving the accuracy and precision of the recovery data;

[0032] 3. The main work device of the present application is on the vibration disc 2, and the transformation of the water bath pot body 1 is small, so the daily disinfection, sterilization and maintenance of the water bath pot are simple;

[0033] 4. The freezing tube is vibrated in a certain space below the liquid surface of the water bath when thawing, and the vibration device 4 parameters can be adjusted, which can avoid water splashing caused by large vibration amplitude during recovery, and reduce the laboratory cleaning and maintenance cost;

[0034] 5. The O-shaped sealing ring 3 fixes the freezing tube in a certain range, which is convenient for the experimenter to observe the thawing process of the frozen cells through the transparent liquid surface, and the frozen cells are heated more uniformly and stably compared with the traditional recovery method of taking out the freezing tube from the water body of the water bath for observation.

[0035] In order to make the vibration disc 2 more stable, as a preferred, the utility model discloses two thread connection seats 11 arranged left and right are connected on the front and back sides of the vibration disc 2, the thread connection seat 11 is all screw thread connection has the installation rod 12, the installation rod 12 end is all connected with the hanging rod 13, the hanging rod 13 end is all screw thread connection has the limit cap 14, the hanging rod 13 hangs on the kettle body 1, and is limited through the limit cap 14 and the installation rod 12, the utility model is through the cooperation of the limit cap 14 and the installation rod 12 to limit the vibration disc 2, makes the vibration disc 2 more stable.

Claims

1. A shaking water bath for cell recovery, comprising a bath body (1), characterized in that: It also includes the vibration disc (2); the vibration disc (2) is vertically inserted into the pot body (1), and the vibration disc (2) is provided with a mounting hole in the part located in the pot body (1), and the vibration disc (2) is provided with a vibration device (4), a power supply (5) and a control panel (6) in the part located above the pot body (1); the control panel (6) is connected with the vibration device (4) and the power supply (5) respectively; the mounting hole is provided with an O-shaped sealing ring (3).

2. A shaking water bath for cell recovery according to claim 1, wherein: The bottom of the pot body (1) is provided with a clamping groove (7); the lower part of the vibration disc (2) is inserted into the clamping groove (7).

3. A shaking water bath for cell recovery as claimed in claim 1, wherein: The vibration device (4) is a vibration motor.

4. A shaking water bath for cell recovery as claimed in claim 1, wherein: The front and rear sides of the vibration disc (2) are both connected with two left and right arranged threaded connecting seats (11); the threaded connecting seats (11) are all threadedly connected with mounting rods (12); the mounting rods (12) are all connected with hanging rods (13) at the ends; the hanging rods (13) are all threadedly connected with limiting caps (14) at the ends; the hanging rods (13) are hung on the pot body (1), and are limited by the limiting caps (14) and the mounting rods (12).

5. A shaking water bath for cell recovery as claimed in claim 1, wherein: The thickness of the vibration disc (2) is less than or equal to 1cm, and the material is stainless steel which is convenient for conducting vibration.

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