Cell co-culture experimental device capable of easily changing liquid

Abstract

The utility model relates to a cell co-culture experimental device easy to change liquid, which comprises a culture plate, and a liquid inlet device is arranged on the side wall of the culture plate close to the bottom so as to discharge new liquid into the culture plate; a liquid discharging device is arranged at the bottom of the culture plate and is used for discharging old liquid in the culture plate. The liquid inlet device comprises a liquid inlet pipe arranged on the side wall of the culture plate, the liquid inlet pipe is obliquely arranged, the liquid inlet direction inclines to the bottom of the culture plate, and an arc-shaped protrusion opposite to a liquid inlet outlet of the liquid inlet pipe is arranged on a bottom plate of the culture plate. The liquid discharging device comprises liquid discharging pipes arranged at the bottom of the culture plate, and the multiple liquid discharging pipes intersect and communicate with a liquid collecting pipe arranged at the bottom of the culture plate. According to the cell co-culture device, cell pollution can be avoided on the basis of quickly realizing cell liquid change, and meanwhile, the cell co-culture process is simplified.

Description

Technical Field

[0001] This utility model belongs to the field of medical device technology, and in particular relates to a cell co-culture experimental device that is easy to change the medium. Background Technology

[0002] Cell-cell interaction research is a crucial topic in life sciences, playing a significant role in tissue development, immune responses, and the tumor microenvironment. Currently, Transwell co-culture devices are widely used in cell experiments to analyze cell interactions. This process typically requires replacing the nutrient-depleted culture medium after several days of culture. The traditional method involves using forceps to remove the upper chamber from the culture plate, then using a pipette to remove the waste liquid from the lower chamber and replenishing with fresh culture medium. This procedure is not only cumbersome but also prone to contamination. To avoid contamination, the forceps must first undergo pretreatment such as autoclaving before medium replacement. Secondly, during medium replacement, the chamber held by the forceps is exposed to air for an extended period, potentially leading to accidental drop. Even temporarily placing it in another sterile culture plate increases the complexity of the procedure and the risk of contamination.

[0003] Therefore, given the drawbacks of traditional Transwell co-culture devices, such as cumbersome operation and high risk of contamination during long-term culture and medium change, there is an urgent need for a simpler and sterile cell co-culture device to reduce cell contamination and simplify the culture process.

[0004] This invention is proposed against this background, aiming to address the shortcomings of traditional Transwell co-culture devices by designing an improved co-culture device that is easy to operate and has a low risk of contamination, in order to promote cell interaction research and provide researchers in related fields with better experimental technical support. Utility Model Content

[0005] To address the shortcomings of existing Transwell chamber co-culture devices, this invention provides a cell co-culture experimental device that facilitates medium exchange, ensuring rapid cell medium replacement while avoiding cell contamination and simplifying the cell co-culture process.

[0006] A cell co-culture experimental device that is easy to change the medium includes a culture plate, wherein a liquid inlet device is provided on the side wall of the culture plate near the bottom to drain new liquid into the culture plate;

[0007] The bottom of the culture plate is equipped with a drainage device to drain the old liquid inside the culture plate.

[0008] The liquid inlet device includes a liquid inlet pipe disposed on the side wall of the culture plate.

[0009] The liquid inlet pipe is obliquely arranged, and the liquid inlet direction is inclined to the bottom of the culture plate.

[0010] The end of the liquid inlet pipe is provided with a pipe cover.

[0011] The bottom plate of the culture plate is provided with an arc-shaped protrusion, which is arranged close to the side wall of the culture plate and opposite to the liquid inlet outlet of the liquid inlet pipe.

[0012] The arc-shaped protrusion is half-moon shaped.

[0013] The liquid outlet device comprises a liquid outlet pipe arranged at the bottom of the culture plate, and a liquid outlet hole is arranged on the bottom plate of the culture plate and corresponds to the liquid outlet pipe.

[0014] The liquid outlet pipe is uniformly distributed along the circumference of the bottom plate of the culture plate, and a plurality of liquid outlet pipes are arranged in a diverging manner from the center to the periphery at the bottom of the culture plate.

[0015] The plurality of liquid outlet pipes converge and are connected to a liquid collecting pipe arranged at the bottom of the culture plate.

[0016] The liquid outlet pipe is provided with a control valve for opening and closing the liquid outlet pipe.

[0017] The beneficial effects of the present application are:

[0018] The present application can quickly change the liquid of cells, and can avoid the difficulty of liquid changing operation caused by the existence of Transwell chamber, and the cell pollution caused by accidental touch during liquid changing operation.

[0019] The present application is extremely simple in design, easy to change liquid, low pollution risk, practical effect is remarkable, the operation process is simple and smooth, and is developed on the basis of experimental operation demand, has strong practicality, has higher practical value.

[0020] The present application can not only improve the experimental efficiency, but also has important significance for ensuring the quality of experimental data; at the same time, it is also the urgent demand of the field of life science research, in order to promote the research of cell interaction, and provide better experimental technical support for researchers in related fields. BRIEF DESCRIPTION OF DRAWINGS

[0021] Fig. 1 The present application is extremely simple in design, easy to change liquid, low pollution risk, practical effect is remarkable, the operation process is simple and smooth, and is developed on the basis of experimental operation demand, has strong practicality, has higher practical value.

[0022] Fig. 2 The present application is extremely simple in design, easy to change liquid, low pollution risk, practical effect is remarkable, the operation process is simple and smooth, and is developed on the basis of experimental operation demand, has strong practicality, has higher practical value.

[0023] Fig. 3 The present application is extremely simple in design, easy to change liquid, low pollution risk, practical effect is remarkable, the operation process is simple and smooth, and is developed on the basis of experimental operation demand, has strong practicality, has higher practical value.

[0024] wherein,

[0025] 1 - liquid discharge device, 10 - liquid discharge hole, 2 - liquid inlet device, 3 - support rod, 4 - liquid discharge pipe, 5 - liquid collection pipe, 6 - control valve, 7 - liquid inlet pipe, 8 - pipe cover, 9 - arc-shaped protrusion. DETAILED DESCRIPTION

[0026] In order to better explain the utility model, so as to facilitate understanding, the following combining with the drawings, through specific embodiments, the technical scheme and effect of the utility model are described in detail.

[0027] As Figs. 1-3 shown, a cell co-culture experimental device easy to change liquid, including culture plate, the culture plate side wall is close to the bottom place is provided with liquid inlet device 2, to the culture plate inside new liquid is discharged. The culture plate bottom is provided with liquid discharge device 1, to discharge the old liquid in the culture plate inside.

[0028] The liquid inlet device 2 includes a liquid inlet pipe 7 provided on the side wall of the culture plate, and the liquid inlet pipe 7 is inclinedly arranged, and the liquid inlet direction is inclined to the bottom of the culture plate. The end of the liquid inlet pipe 7 is provided with a pipe cover 8 for plugging the liquid inlet pipe 7.

[0029] In the embodiment, the liquid inlet device 2 includes a liquid inlet pipe 7, which is arranged on the side wall of the culture plate and is used to discharge new cleaning liquid or cell culture liquid into the culture plate. The liquid inlet pipe 7 is inclinedly arranged, and the inclination direction is such that the liquid inlet direction is towards the bottom of the culture plate. The end of the liquid inlet pipe 7 is provided with a pipe cover 8 to plug the liquid inlet pipe 7 in the non-liquid inlet state. When it is needed to discharge liquid into the culture plate, it can be screwed off from the liquid inlet pipe 7.

[0030] The bottom plate of the culture plate is provided with an arc-shaped protrusion 9, which is arranged close to the side wall of the culture plate and opposite to the liquid inlet outlet of the liquid inlet pipe 7.

[0031] In the embodiment, the bottom plate of the culture plate is provided with an arc-shaped protrusion 9, which is in half-moon shape, arranged close to the side wall of the culture plate, opposite to the liquid inlet direction of the liquid inlet pipe 7, and opposite to the liquid inlet outlet of the liquid inlet pipe 7. In use, when the liquid inlet pipe 7 is used to discharge cleaning liquid or cell culture liquid into the culture plate, the liquid inlet pipe 7 is inclinedly downward along the liquid inlet pipe, and the gun head can be abutted on the arc-shaped protrusion 9 to prevent the surrounding adherent cells from being washed down, so as to slowly inject the liquid.

[0032] The liquid drainage device 1 comprises a plurality of liquid drainage pipes 4 arranged on the bottom of the culture plate, and the liquid drainage pipes 4 are arranged in a diverging manner from the center to the periphery of the culture plate. The bottom of the culture plate is provided with a plurality of liquid drainage holes 10 corresponding to the liquid drainage pipes 4. The liquid drainage pipes 4 are connected to a liquid collecting pipe 5 arranged on the bottom of the culture plate to drain the old culture liquid in the culture plate to the outside. The liquid drainage pipes 4 are provided with control valves 6 for opening and closing the liquid drainage pipes 4.

[0033] In the embodiment, the liquid drainage device 1 comprises a plurality of liquid drainage pipes 4 arranged on the bottom of the culture plate. The liquid drainage pipes 4 are arranged in a circumferential manner on the bottom of the culture plate, and the liquid drainage pipes 4 are arranged in a diverging manner from the center to the periphery of the culture plate. The bottom of the culture plate is provided with liquid drainage holes 10 corresponding to the liquid drainage pipes 4, and the liquid drainage holes 10 are arranged along the edge of the bottom of the culture plate. The ends of the liquid drainage pipes 4 are connected to a liquid collecting pipe 5 for collecting the old liquid in the culture plate and draining the old liquid to the outside. The liquid collecting pipe 5 is arranged on the bottom of the culture plate, and the end of the liquid collecting pipe 5 is provided with a control valve 6. When the control valve 6 is opened, the liquid collecting pipe 5 can drain the liquid to the outside of the culture plate. When the liquid is not needed to be drained to the outside, the control valve 6 is closed.

[0034] The bottom of the culture plate is provided with a support rod 3 for fixing and supporting the culture plate.

Claims

1. A cell co-culture experimental device easy to change liquid, comprising a culture plate, characterized in that: The side wall of the culture plate is provided with a liquid inlet device (2) near the bottom to discharge new liquid into the culture plate; The bottom of the culture plate is provided with a liquid outlet device (1) to discharge old liquid inside the culture plate.

2. The cell co-culture experimental device easy to change liquid according to claim 1, wherein: The liquid inlet device (2) comprises a liquid inlet pipe (7) arranged on the side wall of the culture plate.

3. The cell co-culture experimental device easy to change liquid according to claim 2, wherein: The liquid inlet pipe (7) is arranged obliquely, and the liquid inlet direction is inclined to the bottom of the culture plate.

4. The cell co-culture experimental device easy to change liquid of claim 2, wherein: The end of the liquid inlet pipe (7) is provided with a pipe cover (8).

5. The cell co-culture experimental device easy to change liquid of claim 2, wherein: The bottom plate of the culture plate is provided with an arc-shaped protrusion (9) arranged near the side wall of the culture plate and opposite to the liquid inlet outlet of the liquid inlet pipe (7).

6. The cell co-culture experimental device easy to change liquid according to claim 5, wherein: The arc-shaped protrusion (9) is in the shape of a half moon.

7. The cell co-culture experimental device easy to change liquid of claim 1, wherein: The liquid outlet device (1) comprises a liquid outlet pipe (4) arranged on the bottom of the culture plate, and a liquid outlet hole (10) is formed on the bottom plate of the culture plate corresponding to the liquid outlet pipe (4).

8. The cell co-culture experimental device easy to change liquid according to claim 7, characterized in that: The liquid outlet pipe (4) is uniformly distributed along the circumference of the bottom plate of the culture plate, and a plurality of liquid outlet pipes (4) are arranged in a diverging manner from the center to the periphery of the bottom of the culture plate.

9. The cell co-culture experimental device easy to change liquid of claim 7, wherein: A plurality of liquid outlet pipes (4) intersect and communicate with a liquid collecting pipe (5) arranged on the bottom of the culture plate.

10. The cell co-culture experimental device easy to change liquid of claim 7, wherein: The liquid outlet pipe (4) is provided with a control valve (6) for opening and closing the liquid outlet pipe (4).

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