Ultrasonic irradiation system for irregular part of mouse
By designing an ultrasound irradiation system for irregular parts of mice, the problem of ultrasound irradiation of irregular parts of mice in existing technologies has been solved, achieving precise and stable ultrasound irradiation, improving experimental efficiency and the reliability of results, and meeting ethical requirements.
Patent Information
- Application Number
- CN202422701869.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-06
- Publication Date
- 2025-11-28
- Estimated Expiration
- 2034-11-06
AI Technical Summary
Existing technologies lack effective ultrasound irradiation systems, especially in studies of biological effects on irregular areas of mouse tissue. It is difficult to achieve precise and stable ultrasound irradiation, particularly in studies of irregular areas of adipose tissue such as groin fat, epididymal fat, back fat, and neck fat, where the procedure is complex and time-consuming.
An ultrasound irradiation system for irregular areas of mice was designed, including an anesthesia machine, ultrasound equipment, and a fixation plate. The fixation plate is equipped with a mask and an ultrasound output positioning bracket. By combining the ultrasound output positioning bracket and the mouse fixation plate, precise fixation and ultrasound irradiation of irregular areas of mice can be achieved. The anesthesia mask is equipped to reduce stress response in mice.
This method enables precise ultrasound irradiation of irregular areas in mice, reducing cumbersome procedures, improving experimental efficiency and the reliability of results, meeting ethical requirements for animal experiments, and reducing data bias and operating costs.
Smart Images
Figure CN223601885U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to experimental equipment technical field especially, relates to a mouse irregular part ultrasonic irradiation system. BACKGROUND
[0002] In recent years, the application of ultrasound in clinical treatment is increasing, in addition to the traditional tumor ablation treatment, it promotes soft tissue regeneration, promotes bone healing, relieves osteoporosis and improves drug delivery efficiency, which has great application potential, and is paid more and more attention by researchers. Although ultrasound has the advantages of noninvasive, targeted and noninvasive in treatment, but its safety and effectiveness in various treatment methods, especially in emerging ultrasound treatment, still need to be verified, so it is urgent to study the biological effects of ultrasound.
[0003] However, at present, in the study of the biological effects of ultrasound, especially in the study of the biological effects of irregular parts, there is a lack of effective ultrasonic irradiation system. The construction of these systems is complex, and it is difficult to achieve effective ultrasonic irradiation in technology, and this problem is particularly prominent in the study of adipose tissue. The irregular regions in adipose tissue, such as inguinal fat, epididymal fat, back fat and neck fat, all have complex three-dimensional shape, which brings great challenge to ultrasonic irradiation.
[0004] Taking the most common inguinal fat area of mice in experiments as an example, because the inguinal fat area of mice has a specific V-shaped structure, and the mice need to lie on their backs and keep their faces up during the experiment, the experimental personnel usually need to hold the ultrasonic transducer to irradiate it. This operation method not only increases the difficulty and operation burden of the experiment, but also constitutes a significant limitation for long-term and multi-cycle irradiation experiments.
[0005] Therefore, it is necessary to provide a mouse irregular part ultrasonic irradiation system to solve the above technical problems. UTILITY MODEL CONTENT
[0006] In view of the above situation, in order to overcome the defects of the prior art, the utility model provides a mouse irregular part ultrasonic irradiation system which can effectively help the animal experiment research of related ultrasonic effect, thereby providing a powerful tool for the basic research of ultrasonic treatment.
[0007] In order to achieve the above purpose, the utility model adopts the following technical scheme:
[0008] The mouse irregular part ultrasonic irradiation system comprises an anesthetizing machine, an ultrasonic device and a fixing plate, the fixing plate is provided with a mask and an ultrasonic output positioning support, the mask is connected with the anesthetizing machine through a pipeline, in use, the mask is covered on the head of the mouse, so that the mouse is kept in an anesthetized state, the ultrasonic output positioning support comprises a base, the base is nailed on the fixing plate through a plurality of nails, a stand is fixedly arranged on the top of the base close to the edge of one side, a sleeve is movably sleeved on the stand, a fixing assembly is arranged on one side of the sleeve, the fixing assembly can fix the sleeve on the stand, a connecting rod is fixedly installed on one side of the sleeve, a rotating rod is rotatably installed on the end of the connecting rod away from the sleeve, the connecting rod and the rotating rod are connected through a connecting portion, under the action of the connecting portion, the rotating rod can be adjusted at a proper angle and kept fixed after adjustment, a clamp assembly is installed on one end of the rotating rod, and the clamp assembly is used for fixing the transducer of the ultrasonic device.
[0009] Preferably, the fixing assembly comprises a threaded pipe fixedly arranged on the sleeve, a fixing screw is screwedly installed in the threaded pipe, and the fixing screw can extend into the sleeve, and when the fixing screw extends into the sleeve, the fixing screw abuts against the stand, so as to fix the sleeve.
[0010] Preferably, the clamp assembly comprises a fixed clamp and a movable clamp fixed with the fixed rotating rod, and the fixed clamp and the movable clamp are fixed through two bolts.
[0011] Preferably, the connecting portion is arranged at one end of the connecting rod, the connecting portion comprises an installation groove formed in the connecting rod, a friction block is slidably installed in the installation groove, an arc-shaped friction surface is formed in one side of the friction block, the arc-shaped friction surface is matched with one end of the rotating rod, when the rotating rod needs to be fixed, the friction block is moved to make the arc-shaped friction surface tightly contact with one end of the rotating rod, and the rotating rod is fixed under the compression of the arc-shaped friction surface, an inclined groove is formed in one side of the friction block away from the arc-shaped friction surface, a jack screw is screwedly installed on the connecting rod and extends into the inclined groove, two springs are fixedly installed on one side of the friction block away from the arc-shaped friction surface, and the springs are fixed with the installation groove.
[0012] Preferably, a notch is formed in the mask, the notch is used for adapting the neck of the mouse, when the mouse lies on the fixing plate, the mask is arranged on the head of the mouse, and the notch is clamped at the neck of the mouse, and the two sides of the mask are provided with inserting nails, the inserting nails can be inserted on the fixing plate, and the inserting nails are helpful to fix the mask.
[0013] Preferably, the mask is made of polyester plastic, is trumpet-shaped, and has a thickness of 0.5 mm. The inside is fixed with a medical cotton ball by tape to prevent the mouse head from falling off during anesthesia. The shape and thickness of the medical cotton ball can be adjusted to adapt the anesthesia mask to most mouse heads. When the mask is connected to the anesthesia machine, the gap at the joint is sealed with a sealing film to ensure air tightness.
[0014] Preferably, the fixing plate is made of polystyrene foam plate combined with a disposable sterile medical surgical pad. The polystyrene foam plate facilitates the quick and convenient insertion of nails and nails into the fixing plate. The use of a disposable sterile medical surgical pad can keep the device clean and hygienic, as the disposable sterile medical surgical pad needs to be discarded after each experiment.
[0015] Preferably, the fixing plate is provided with a fixing groove matched with the mouse. The laboratory needs to place the mouse in the fixing groove. The purpose of setting the placing groove is to help fix the mouse. After the mouse is fixed in position, the ultrasonic output positioning support is also fixed in position, so that it is not necessary to adjust the ultrasonic output positioning support repeatedly during multiple experiments.
[0016] Compared with the prior art, the utility model has the following beneficial effects:
[0017] (1) The utility model can effectively help the animal experiment research of relevant ultrasonic effect, thereby providing a powerful tool for the basic research of ultrasonic treatment.
[0018] (2) The utility model discloses an ultrasonic output positioning support and a mouse fixing plate, which can accurately adjust and fix the position of ultrasonic output, ensure stable operation in long-time experiments, guarantee the accuracy of ultrasonic irradiation, and significantly reduce the tedious steps of frequent manual adjustment. By flexibly adjusting the angle and position of the ultrasonic output positioning support and the mouse, and filling a sufficient amount of coupling agent in the gap, effective irradiation of various irregular areas such as inguinal fat, epididymal fat, back fat and neck fat can be realized.
[0019] (3) The utility model not only greatly improves the experimental efficiency, but also reduces the data deviation caused by unstable operation, thereby significantly improving the reliability and repeatability of experimental results.
[0020] (4) The anesthesia mask provided in the system can maintain the long-time anesthesia state of the mouse, effectively reduce the stress reaction and discomfort of the mouse, meet the ethical requirements of animal experiments, and further improve the social acceptance of the experiment.
[0021] (5) The devices in the utility model are made of common materials, have a simple structure, are low in cost, are convenient to assemble and maintain, and have good economy and sustainability. BRIEF DESCRIPTION OF DRAWINGS
[0022] Figure 1 Part structure schematic view of mouse irregular part ultrasonic irradiation system is provided for the utility model,
[0023] Figure 2 Part structure schematic view of mouse irregular part ultrasonic irradiation system is provided for the utility model,
[0024] Figure 3 Part structure schematic view of mouse irregular part ultrasonic irradiation system is provided for the utility model,
[0025] Figure 4 Sectional view structure schematic view of connecting portion in mouse irregular part ultrasonic irradiation system is provided for the utility model,
[0026] Figure 5 Exploded schematic view of connecting portion in mouse irregular part ultrasonic irradiation system is provided for the utility model.
[0027] Wherein, the name corresponding to the reference sign is: 1-fixed plate, 2-face shield, 3-, 4-base, 5-nail, 6-nail, 7-sleeve, 8-, 9-, 10-connecting rod, 11-rotating rod, 12-fixed clamp, 13-movable clamp, 14-mounting groove, 15-friction block, 16-arc friction surface, 17-inclined slot, 18-setscrew, 19-spring, 20-fixed groove. DETAILED DESCRIPTION
[0028] The utility model will be further explained in combination with the drawings and examples, and the mode of the utility model includes but is not limited to the following examples.
[0029] Example 1
[0030] As Figures 1-5As shown, the ultrasonic irradiation system for irregular parts of mice provided by this utility model includes: an anesthesia machine, an ultrasonic device, and a fixing plate 1. A mask 2 and an ultrasonic output positioning bracket are mounted on the fixing plate 1. The mask 2 is connected to the anesthesia machine via tubing. In use, the mask covers the mouse's head to keep the mouse in anesthetized state. The ultrasonic output positioning bracket includes a base 4, which is nailed to the fixing plate 1 by multiple nails 5. A column 6 is fixedly mounted on the top edge of the base 4 near one side. A sleeve 7 is movably fitted onto the column 6. A fixing component is provided on one side of the sleeve 7, which can fix the sleeve 7 to the column 6. A connecting rod 10 is fixedly installed on one side of the sleeve 7. A rotating rod 11 is rotatably installed on the end of the connecting rod 10 away from the sleeve 7. The connecting rod 10 and the rotating rod 11 are connected by a connecting part. Under the action of the connecting part, the rotating rod 11 can be adjusted at an appropriate angle and can maintain a fixed rotation after adjustment. A clamping component is installed on one end of the rotating rod 11. The clamping component is used to fix the transducer of the ultrasonic equipment.
[0031] During the experiment, mice were placed in the anesthesia chamber of an anesthesia machine. After approximately one minute, the mice were anesthetized. They were then removed from the anesthesia chamber, and their heads were secured within the mask 2. The anesthesia machine was adjusted to an appropriate dosage to maintain a stable anesthesia state during the experiment. Next, the mice were positioned supine, exposing both groins. Medical tape was used to secure the mice's hind limbs to the bottom of the fixation plate, ensuring stability. The transducer of the ultrasound device was fixed to the clamp assembly, and the height and angle of the ultrasound output positioning bracket were adjusted to ensure precise alignment of the transducer with the left groin area of the mouse. The base 4 was then nailed to the fixation plate 1 using multiple nails 5. Sufficient ultrasound coupling agent was applied to the left groin, ensuring that air bubbles would not interfere with ultrasound transmission. After confirming that the ultrasound transducer and the mouse groin were completely filled with coupling agent, the ultrasound equipment was started for irradiation.
[0032] Example 2
[0033] like Figure 2 As shown, the fixing component includes a threaded tube 8 fixedly mounted on the sleeve 7. A fixing screw 9 is installed inside the threaded tube 8. The fixing screw can extend into the sleeve 7. When the fixing screw extends into the sleeve 7, it will push against the column 6 and fix the sleeve 7. When the fixing component loses its fixation on the sleeve 7, the sleeve 7 can rotate or be adjusted up and down on the column 6, thereby realizing the adjustment of the clamp component, that is, realizing the adjustment of the ultrasonic transducer in the horizontal and vertical directions.
[0034] Example 3
[0035] like Figure 2As shown, the clamp assembly includes a fixed clamp 12 and a movable clamp 13 that are fixed to the fixed rotating rod 11. The fixed clamp 12 and the movable clamp 13 are fixed by two bolts.
[0036] Example 4
[0037] like Figures 4-5 As shown, the connecting part is located at one end of the connecting rod 10. The connecting part includes a mounting groove 14 formed on the connecting rod 10. A friction block 15 is slidably installed inside the mounting groove 14. An arc-shaped friction surface 16 is formed on one side of the friction block 15. The arc-shaped friction surface 16 matches one end of the rotating rod 11. When it is necessary to fix the rotating rod 11, the friction block 15 is moved so that the arc-shaped friction surface 16 is in close contact with one end of the rotating rod 11. The rotating rod 11 is pressed against the arc-shaped friction surface. The friction block 15 is fixed at the bottom. A groove 17 is provided on the side of the friction block 15 away from the arc-shaped friction surface 16. A set screw 18 is threaded on the connecting rod 10. A certain part of the set screw 18 extends into the groove 17. When the set screw 18 is rotated to move into the groove 17, the set screw 18 will contact the groove 17 and push the friction block 15 to one side. Two springs 19 are fixedly installed on the side of the friction block 15 away from the arc-shaped friction surface 16. The springs 19 are fixed to the mounting groove 14.
[0038] Example 5
[0039] like Figure 1 As shown, the mask 2 has a notch 3, which is used to fit the mouse's neck. When the mouse lies on the fixing plate 1, the mask 2 shines on the mouse's head, and the notch 3 is locked at the mouse's neck. The mask 2 has pins on both sides, which can be inserted into the fixing plate 1. The pins help to fix the mask 2.
[0040] Example 6
[0041] The mask 2 is made of polyester plastic, is trumpet-shaped, and 0.5 mm thick. Medical cotton balls are secured inside with tape to prevent the mouse's head from falling off during anesthesia. The shape and thickness of the cotton balls can be adjusted to fit most mouse heads. When connecting the mask 2 to the anesthesia machine, a sealing film is used to seal the interface to ensure airtightness.
[0042] Example 7
[0043] The fixing plate 1 is made of polystyrene foam board and disposable sterile medical surgical pad. The use of polystyrene foam board makes it easy to quickly and conveniently insert nails 5 and pins into the fixing plate 1. The use of disposable sterile medical surgical pad can keep the device clean and hygienic, because the disposable sterile medical surgical pad needs to be discarded after each experiment.
[0044] Example 8
[0045] like Figure 1 As shown, the fixing plate 1 has a fixing slot 20 adapted to the mouse. The laboratory needs to place the mouse in the fixing slot. The purpose of the slot is to help fix the mouse. Simultaneously, once the mouse's position is fixed, the position of the ultrasound output positioning bracket is also fixed, thus eliminating the need for repeated adjustments of the ultrasound output positioning bracket during multiple experiments. The location of the fixing slot is not fixed and needs to be determined based on the specific location of the mouse's ultrasound irradiation. Since the fixing plate 1 is made of foam board, the design of its fixing slot is relatively convenient and simple.
[0046] In an experiment to promote beige-colored white adipose tissue in mouse groin fat by low-intensity ultrasound irradiation, the experimental design was as follows: the left groin of each mouse received ultrasound irradiation, while the right side served as a control group. Irradiation was performed for 20 minutes daily for 7 consecutive days.
[0047] First, connect the mouse anesthesia mask to the anesthesia machine interface and seal the connection with sealing film to ensure airtightness. The experiment has no special requirements for the anesthesia machine and ultrasound equipment; ensure all equipment is functioning properly before starting the experiment. Place the mouse in the anesthesia chamber of the anesthesia machine. After approximately one minute, the mouse will be anesthetized. At this point, remove the mouse from the anesthesia chamber and fix its head inside the anesthesia mask. Adjust the anesthesia machine to an appropriate dosage to maintain a stable anesthetized state for the mouse during the experiment.
[0048] Next, adjust the mouse's position so that it lies supine, exposing both groins. Use medical tape to secure the mouse's hind limbs to the bottom of the fixation board to ensure its stability.
[0049] Apply sufficient ultrasound coupling agent to the left groin area, ensuring that air bubbles do not interfere with ultrasound transmission. Adjust the height and angle of the ultrasound output positioning bracket to ensure the transducer is precisely aligned with the mouse's left groin region. Once it is confirmed that the ultrasound transducer and the mouse's groin are completely filled with coupling agent, start the ultrasound machine to begin irradiation. After the experiment, discard the disposable sterile surgical pad immediately to maintain the cleanliness and hygiene of the apparatus.
[0050] Once the system is set up, it requires almost no further adjustments throughout the entire experimental period. The preparation time between each pair of experimental mice can be controlled within 2 minutes, and with increased operator proficiency, the preparation time can even be reduced to about 1 minute. The design significantly reduces the time and effort spent by researchers on mouse fixation and ultrasonic transducer angle adjustment, thereby improving experimental efficiency.
Claims
1. A mouse irregular site ultrasonic irradiation system, characterized by, The utility model relates to anesthetic machine, ultrasonic equipment and fixed plate (1), be provided with face guard (2) and ultrasonic output positioning support on fixed plate (1), face guard (2) is connected with anesthetic machine through pipeline, the ultrasonic output positioning support includes base (4), the base (4) is nailed on fixed plate (1) through a plurality of nails (5), the top near the edge of base (4) one side is fixedly installed with stand (6), the stand (6) is movably sleeved with sleeve (7), one side of sleeve (7) is provided with fixed assembly, fixed assembly can fix sleeve (7) on stand (6), one side of sleeve (7) is fixedly installed with connecting rod (10), the one end away from sleeve (7) of connecting rod (10) is rotatably installed with rotating rod (11), and connecting rod (10) is connected between rotating rod (11) through connecting portion, and one end of rotating rod (11) is installed with clamp assembly, and clamp assembly is used for fixing the transducer of ultrasonic equipment. The fixed assembly includes a threaded tube (8) fixedly arranged on the sleeve (7), and a fixing screw (9) is threadedly installed in the threaded tube (8), and the fixing screw can extend into the sleeve (7).
2. The mouse irregular site ultrasonic irradiation system according to claim 1, wherein The clamp assembly includes a fixed clamp (12) and a movable clamp (13) fixed with the rotating rod (11), and the fixed clamp (12) and the movable clamp (13) are fixed by two bolts.
3. The mouse irregular site ultrasonic irradiation system according to claim 1, wherein The connecting portion is arranged at one end of the connecting rod (10), and the connecting portion includes a mounting groove (14) formed in the connecting rod (10), a friction block (15) is slidably installed in the mounting groove (14), an arc-shaped friction surface (16) is formed in one side of the friction block (15), the arc-shaped friction surface (16) is matched with one end of the rotating rod (11), an inclined groove (17) is formed in one side of the friction block (15) away from the arc-shaped friction surface (16), a jackscrew (18) is threadedly installed on the connecting rod (10), one end of the jackscrew (18) extends into the inclined groove (17), two springs (19) are fixedly installed on one side of the friction block (15) away from the arc-shaped friction surface (16), and the springs (19) are fixed with the mounting groove (14).
4. The mouse irregular site ultrasonic irradiation system according to claim 1, wherein A notch (3) is formed in the face guard (2), and the notch (3) is used for adapting the neck of a mouse, when the mouse lies on the fixed plate (1), the face guard (2) is placed on the head of the mouse, and the notch (3) is clamped on the neck of the mouse, and the face guard (2) is provided with a plug on both sides.
5. The mouse irregular site ultrasonic irradiation system according to claim 1, wherein The face guard (2) is made of polyester plastic and has a horn shape, and the thickness is 0.5 mm, the inside of the face guard (2) is fixed with a medical cotton ball by using adhesive tape, the shape and thickness of the medical cotton ball are adjusted to adapt the face guard (2) to the head of most mice, and when the face guard (2) is connected with the anesthetic machine, a sealing film is used to seal the gap between the face guard (2) and the anesthetic machine.
6. The mouse irregular site ultrasonic irradiation system according to claim 1, wherein The fixed plate (1) is made of polystyrene foam and a disposable sterile medical operating pad.
7. The mouse irregular site ultrasonic irradiation system according to claim 1, wherein A fixing groove (20) adapted to the mouse is formed in the fixed plate (1).
8. The mouse irregular site ultrasonic irradiation system according to claim 1, wherein