Revolution mechanism and purification device thereof
By combining the orbital mechanism and the spin reaction unit, the problem of poor mixing effect of magnetic bead reagents in small volume liquids is solved, achieving more efficient mixing and uniformity, and adapting to the needs of different solutions and magnetic beads.
Patent Information
- Application Number
- CN202423074916.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-12
- Publication Date
- 2025-11-28
- Estimated Expiration
- 2034-12-12
AI Technical Summary
In the existing technology, magnetic bead reagents are not effective at mixing small volumes of liquid, are not easy to mix at low frequencies, and are prone to affecting the product at high frequencies. They also have problems with liquid splashing and bubbles, and the speed adjustment range of the mixer is small.
It employs a revolution mechanism and a spin reaction unit to improve the mixing effect by combining spin and revolution. The system includes components such as a revolution drive unit, a spin reaction unit, and an electromagnet to realize the spin revolution of multiple reaction units, adapting to the mixing requirements of solutions with different viscosities and magnetic bead diameters.
It improves the uniformity and mixing efficiency of the reaction liquid, reduces experimental differences, adapts to the mixing effect of different solution viscosities and magnetic bead diameters, and ensures that the reaction proceeds fully.
Smart Images
Figure CN223602380U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the technical field of purification equipment, especially to the revolution mechanism and its purification device. BACKGROUND
[0002] The magnetic bead reagent is widely used in the sample analysis field. The magnetic bead reagent usually includes magnetic beads, its marker and buffer, and is sucked by the corresponding reagent suction device and is sent to the corresponding reaction container during use. In the prior art, the mixing of small volume liquid is suitable, the mixing of magnetic beads is not easy at low frequency, the product is affected at high frequency, and the technical problems of liquid splashing and bubbles and the like occur;
[0003] Meanwhile, the mixing machine adopts the fixed speed ratio of spin and revolution, and the speed is adjusted in a certain range (500-1500 rpm), and the adjustment range is small. UTILITY MODEL CONTENTS
[0004] To solve the above technical problems, one of the technical solutions of the utility model is: a revolution mechanism, which is used for realizing self-rotation of a plurality of spin reaction units on a purification device, improving mixing effect, and comprising:
[0005] A mounting plate 1;
[0006] A revolution cylinder 2 located in the shell of the purification device; the mounting plate 1 is located at the upper end of the revolution cylinder 2;
[0007] A revolution driving unit 3 for driving the revolution cylinder 2 to rotate, which is installed on the shell of the purification device; wherein the plurality of spin reaction units on the purification device are circularly arranged on the edge of the mounting plate 1.
[0008] Further, the revolution driving unit 3 comprises:
[0009] A revolution driving motor 301 installed on the shell of the purification device;
[0010] A revolution driving motor 301 installed on the shell of the purification device;
[0011] A revolution driving motor 301 installed on the shell of the purification device;
[0012] Further, the revolution driving motor 301 is installed on the shell of the purification device through a clamp 4.
[0013] Further, a plurality of bearings 5 are arranged between the revolution cylinder 2 and the shell of the purification device from top to bottom.
[0014] The utility model discloses a technical scheme among one of which is: purification device, the device includes the revolution mechanism, the device still includes a plurality of spin reaction unit 6.
[0015] Further, the plurality of spin reaction units 6 are all installed on the mounting plate 1 according to the specified 45° angle.
[0016] Further, each spin reaction unit 6 is used for heating and mixing the reagent in the reaction test tube uniformly, so that the reagent is fully reacted.
[0017] The upper mounting shell 601 and the lower mounting shell 602 are used for bearing the device components; the upper mounting shell 601 and the lower mounting shell 602 are connected through the three-hole connecting piece 603;
[0018] The stepped shell 604 is located in the upper mounting shell 601 and divides the upper mounting shell 601 into the inner cavity 605 and the outer cavity 606;
[0019] The rotary body 607 is installed in the inner cavity 605 and the lower mounting shell 602 and is used for rotating the reaction test tube;
[0020] The elastic buckle 608 is located at the upper end of the rotary body 607, and the reaction test tube is mounted on and / or removed from the rotary body 607 through the pressing of the elastic buckle 608;
[0021] The spin driving mechanism 609 is used for providing power for the rotary body 607, and the spin driving mechanism 609 is installed in the outer cavity 606 and the lower mounting shell 602.
[0022] The electromagnet 610 is fixedly installed in the rotary body 607 and located below the reaction test tube, so that the electromagnet 610 rotates synchronously with the rotary body 607; when the electromagnet 610 is powered, heat is generated to heat the reaction test tube; when the electromagnet 610 is powered, a magnetic field is generated to produce adsorption on the magnetic beads in the reaction test tube, so that the magnetic beads are fixed at the bottom of the test tube; when the positive and negative poles of the high-frequency conversion electromagnet 610 are powered, the magnetic field direction is high-frequency switched, and the mixing effect of the magnetic beads is improved;
[0023] The temperature sensor 611 is used for monitoring the heat generated by the electromagnet 610 to realize temperature control; the temperature sensor 611 is fixedly installed in the rotary body 607 and located below the electromagnet 610, so that the temperature sensor 611 rotates synchronously with the rotary body 607;
[0024] The pneumatic connector 612 is installed in the outer cavity 606; the pneumatic connector 612 is connected with the outside through the air pipe, brings out the gas in the gap between the inner cavity 605 and the rotary body 607, and controls the excessive heat generated by the electromagnet 610 in the adjustment process through the adiabatic expansion of the gas.
[0025] Further, the spin driving mechanism 609 comprises:
[0026] A spin driving motor 6091 is installed in the outer cavity 606, and its driving end penetrates through the three-hole connecting piece 603 into the lower mounting shell 602;
[0027] A spin driving gear 6092 is installed on the driving end of the spin driving motor 6091;
[0028] A spin driven gear 6093 is fixedly sleeved on the lower end of the rotary body 607, and is in meshing with the spin driving gear 6092.
[0029] Further, the unit further comprises an electric slip ring 613 for transferring the circuit and signal on the rotary body 607 out, avoiding the twisting phenomenon caused by the rotation of the wire end;
[0030] The electric slip ring 613 is installed on the lower part of the rotary body 607.
[0031] Further, the rotary body 607 and the reaction test tube have at least one ball 614 for reliably fixing the reaction test tube on the upper end of the rotary body 607.
[0032] The utility model has the advantages and positive effects that: when the spin reaction unit 6 is inwardly inclined to a specified angle, such as 45 degrees, the liquid is started to be mixed; then the revolution mechanism is started, the multiple spin reaction units 6 are realized to revolve, the mixing effect is further improved, the liquid reaction in the reaction test tube is fully reacted, the solution is fully mixed, the uniformity of various components in the reaction liquid is ensured, the uniform distribution is more conducive to promoting the reaction, improving the production efficiency, and reducing the experimental difference.
[0033] The speed of the spin and revolution can be arbitrarily adjusted, the speed ratio of the spin and revolution and the different centrifugal forces generated by each other can be controlled, and the solution of different viscosity types and the magnetic beads of different diameter particles can be mixed. BRIEF DESCRIPTION OF DRAWINGS
[0034] Figure 1 It is a structure schematic view of the revolution mechanism;
[0035] Figure 2 It is a rear view of Figure 1
[0036] Figure 3 It is a structure schematic view of the purification device;
[0037] Figure 4 It is a structure schematic view of the spin reaction unit;
[0038] In the figure: 1, mounting plate; 2, revolution cylinder; 3, revolution driving unit; 301, revolution driving motor; 302, revolution driving gear; 303, revolution driven gear; 4, clamp; 5, bearing; 6, self-rotation reaction unit; 601, upper mounting shell; 602, lower mounting shell; 603, three-hole connecting piece; 604, stepped shell; 605, inner cavity; 606, outer cavity; 607, rotary body; 608, elastic buckle; 609, self-rotation driving mechanism; 6091, self-rotation driving motor; 6092, self-rotation driving gear; 6093, self-rotation driven gear; 610, electromagnet; 611, temperature sensor; 612, pneumatic joint; 613, electric slip ring; 614, ball. DETAILED DESCRIPTION
[0039] In order to better understand the present application, the present application will be further described below in conjunction with specific embodiments and drawings.
[0040] Embodiment 1
[0041] As shown in the figure, the revolution mechanism is used to realize self-rotation of the plurality of self-rotation reaction units on the purification device, improve the mixing effect, and the mechanism comprises: Figures 1-2
[0042] The mounting plate 1;
[0043] The revolution cylinder 2 is located in the shell of the purification device; the mounting plate 1 is located at the upper end of the revolution cylinder 2.
[0044] The revolution driving unit 3 is used to drive the revolution cylinder 2 to rotate, which is installed on the shell of the purification device; wherein the plurality of self-rotation reaction units on the purification device are circularly arranged on the edge of the mounting plate 1.
[0045] Further, the revolution driving unit 3 comprises:
[0046] The revolution driving motor 301 is installed on the shell of the purification device;
[0047] The revolution driving gear 302 is installed on the rotating end of the revolution driving motor 301;
[0048] The revolution driven gear 303 is fixedly sleeved on the revolution cylinder 2 and engaged with the revolution driving gear 302.
[0049] Further, the revolution driving motor 301 is installed on the shell of the purification device through the clamp 4.
[0050] Further, a plurality of bearings 5 are provided between the revolution cylinder 2 and the shell of the purification device from top to bottom.
[0051] Its working process: the multiple spin reaction units 6 on the purification device are installed inward at a specified angle. When purification is needed, first turn on the motor in the spin reaction unit 6 to spin the liquid in the reaction test tube. Turn on the drive motor 301 to rotate the driving gear 302, and then rotate the revolving cylinder 2 installed in the driven gear 303, so as to realize the revolution of the multiple spin reaction units 6 on the mounting plate 1, and further improve the mixing effect to make the liquid in the reaction test tube fully react.
[0052] Example 2
[0053] As shown in Figures 3-4 the purification device, the device comprises the revolving mechanism described in the above example 1, and further comprises multiple spin reaction units 6.
[0054] Further, the multiple spin reaction units 6 are all installed inward at a specified 45° angle on the mounting plate 1.
[0055] Further, each of the spin reaction units 6 is used for heating and uniformly mixing the reagent in the reaction test tube to make the reagent fully react; the unit comprises:
[0056] an upper mounting shell 601 and a lower mounting shell 602 for bearing the components of the device; the upper mounting shell 601 and the lower mounting shell 602 are connected through a three-hole connecting piece 603;
[0057] a stepped shell 604 which is located in the upper mounting shell 601 and divides the upper mounting shell 601 into an inner cavity 605 and an outer cavity 606;
[0058] a gyroid 607 which is installed in the inner cavity 605 and the lower mounting shell 602 and is used for rotating the reaction test tube;
[0059] an elastic buckle 608 which is located at the upper end of the gyroid 607 and is used for installing and / or removing the reaction test tube from the gyroid 607 by pressing the elastic buckle 608;
[0060] a spin drive mechanism 609 which is used for providing power for the gyroid 607 and is installed in the outer cavity 606 and the lower mounting shell 602.
[0061] an electromagnet 610 which is fixedly installed in the gyroid 607 below the reaction test tube, rotates synchronously with the gyroid 607, heats the reaction test tube when electrified, and fixes the magnetic beads at the bottom of the test tube when the electromagnet 610 is electrified to generate a magnetic field, and further improves the mixing effect of the magnetic beads by high-frequency switching of the positive and negative poles of the high-frequency conversion electromagnet 610 and the direction of the magnetic field.
[0062] A temperature sensor 611 is arranged in the rotary body 607 below the electromagnet 610 to monitor the heat generated by the electromagnet 610 and to control the temperature.
[0063] A pneumatic joint 612 is arranged in the outer cavity 606 and connected to the outside through a gas pipe to carry out the gas in the gap between the inner cavity 605 and the rotary body 607 and control the excessive heat generated by the electromagnet 610 during the adjustment through the adiabatic expansion of the gas.
[0064] Further, the spin driving mechanism 609 comprises:
[0065] A spin driving motor 6091 is arranged in the outer cavity 606 and its driving end penetrates through the three-hole connecting piece 603 into the lower mounting shell 602.
[0066] A spin driving gear 6092 is arranged on the driving end of the spin driving motor 6091.
[0067] A spin driven gear 6093 is fixedly sleeved on the lower end of the rotary body 607 and engaged with the spin driving gear 6092.
[0068] Further, the unit further comprises an electric slip ring 613 for transferring the circuit and signal on the rotary body 607 to avoid the twisting phenomenon caused by the rotation of the wire end.
[0069] The electric slip ring 613 is arranged on the lower part of the rotary body 607.
[0070] Further, the rotary body 607 and the reaction test tube have at least one ball 614 for reliably fixing the reaction test tube on the upper end of the rotary body 607.
[0071] The working principle is that the reaction test tube is placed on the rotary body 7 by hand, and the reaction test tube is stably clamped on the rotary body 7 by the elastic buckle 8, the magnetic beads and the reaction liquid are put into the reaction test tube, the driving motor 901 is started, the driven gear 903 is driven to rotate through the driving gear 902, and then the rotary body 7 is driven to rotate, so that the rotation of the reaction test tube is realized, and the reaction liquid in the reaction test tube is fully mixed.
[0072] When the spin reaction unit 6 is inclined inward by an angle of 45°, the driving motor 901 is mixed at a mixing speed control of centrifugal acceleration a ≤ 0.8g; the rotation speed can be adjusted during the mixing process, which can enhance the mixing effect.
[0073] When the electromagnet 10 generates heat, the reaction test tube is heated; when the electromagnet 10 generates a magnetic field, the magnetic beads in the reaction test tube are adsorbed to fix the magnetic beads at the bottom of the test tube; when the positive and negative poles of the high-frequency conversion electromagnet 10 are electrified, the direction of the magnetic field is high-frequency switched to improve the mixing effect of the magnetic beads.
[0074] The temperature sensor 11 is used for monitoring the heat generated by the electromagnet 10 to realize temperature control.
[0075] The pneumatic joint 13 is connected with the outside through the air pipe, and the gas in the gap between the inner cavity 5 and the rotary body 7 is taken out and expanded through heat insulation of the gas to control excessive heat generated by the electromagnet 10 in the adjustment process.
[0076] Meanwhile, the speed of the spin and the revolution can be arbitrarily adjusted, and through controlling the speed ratio of the spin and the revolution and the different centrifugal forces generated by each other, the mixing of the magnetic beads of different viscosity types of solutions and different diameter particles can be adapted.
[0077] The above embodiment of the utility model is described in detail, but the content is only the preferred embodiment of the utility model, and cannot be considered as limiting the scope of the utility model. Any equivalent change and improvement within the scope of the utility model should still belong to the scope of the patent.
Claims
1. A revolving mechanism, characterized by: The mechanism is used for realizing self-rotation of multiple spin reaction units on a purification device, improving mixing effect, and the mechanism comprises the following: a mounting plate (1); a revolving cylinder (2) located in a shell of the purification device; the mounting plate (1) is located at an upper end of the revolving cylinder (2); a revolving driving unit (3) for driving the revolving cylinder (2) to rotate, which is installed on the shell of the purification device; wherein multiple spin reaction units on the purification device are circularly arranged on an edge of the mounting plate (1).
2. The gear mechanism according to claim 1, characterized in that: The revolving driving unit (3) comprises: a revolving driving motor (301) installed on the shell of the purification device; a revolving driving motor (301) installed on the shell of the purification device; a revolving driving motor (301) installed on the shell of the purification device; 3. The male transfer mechanism of claim 2, wherein: a revolving driving motor (301) installed on the shell of the purification device.
4. The male transfer mechanism of claim 1, wherein: A plurality of bearings (5) are arranged between the revolving cylinder (2) and the shell of the purification device from top to bottom.
5. A purification device characterized by: The device comprises the revolving mechanism according to any one of claims 1-4, and further comprises multiple spin reaction units (6).
6. The purification device of claim 5, wherein: The multiple spin reaction units (6) are installed on the mounting plate (1) at an angle of 45 degrees.
7. The purification device of claim 5, wherein: Each spin reaction unit (6) is used for heating and uniformly mixing reagents in a reaction test tube, so that the reagents are fully reacted; the unit comprises: an upper mounting shell (601) and a lower mounting shell (602) for bearing components of the device; the upper mounting shell (601) and the lower mounting shell (602) are connected through a three-hole connecting piece (603); a stepped shell (604) arranged in the upper mounting shell (601) and dividing the upper mounting shell (601) into an inner cavity (605) and an outer cavity (606); a rotary body (607) installed in the inner cavity (605) and the lower mounting shell (602) and used for rotating the reaction test tube; an elastic buckle (608) arranged at an upper end of the rotary body (607) and used for installing and / or removing the reaction test tube from the rotary body (607) through pressing of the elastic buckle (608); a spin driving mechanism (609) for providing power for the rotary body (607), which is installed in the outer cavity (606) and the lower mounting shell (602); an electromagnet (610) fixedly installed in the rotary body (607) and located below the reaction test tube, so that the electromagnet (610) rotates synchronously with the rotary body (607); when the electromagnet (610) is powered, heat is generated to heat the reaction test tube; when the electromagnet (610) is powered, a magnetic field is generated to magnetically attract magnetic beads in the reaction test tube and fix the magnetic beads at the bottom of the test tube; when the positive and negative poles of the high-frequency conversion electromagnet (610) are powered, the direction of the magnetic field is high-frequency switched, and the mixing effect of the magnetic beads is improved. A temperature sensor (611) is used to monitor the heat generated by the electromagnet (610) to realize temperature control; the temperature sensor (611) is fixedly installed in the rotary body (607) below the electromagnet (610) so that the temperature sensor (611) rotates synchronously with the rotary body (607); A pneumatic joint (612) is installed in the outer cavity (606); the pneumatic joint (612) is connected with the outside through an air pipe to carry out the gas in the gap between the inner cavity (605) and the rotary body (607) and control the excessive heat generated by the electromagnet (610) in the adjustment process through the adiabatic expansion of the gas.
8. The purification device of claim 7, wherein: The spin driving mechanism (609) comprises: A spin driving motor (6091) is installed in the outer cavity (606) and its driving end penetrates through the three-hole connecting piece (603) into the lower installation shell (602); A spin driving gear (6092) is installed on the driving end of the spin driving motor (6091); A spin driven gear (6093) is fixedly sleeved on the lower end of the rotary body (607) and is in mesh with the spin driving gear (6092).
9. The purification device of claim 7, wherein: The unit also comprises an electric slip ring (613) for transferring the circuit and signal on the rotary body (607) out to avoid the twist phenomenon caused by the rotation of the wire end; The electric slip ring (613) is installed on the lower part of the rotary body (607).
10. The purification device of claim 7, wherein: The rotary body (607) and the reaction test tube have at least one ball (614) between them for reliably fixing the reaction test tube on the upper end of the rotary body (607).