Simple auxiliary film cutting device for Western Blot

Abstract

The utility model provides a simple auxiliary film cutting device used for a Western Blot. The device structurally comprises a base, wherein a base plate is arranged in the middle of the base; strip-shaped grooves are formed in the two sides of the base plate on the base, and two sliding rails are arranged in each strip-shaped groove. A caliper fixing mechanism is connected to the two sliding rails in each strip-shaped groove in a penetrating mode respectively, the two ends of a caliper are clamped to the two caliper fixing mechanisms respectively, and the caliper is used for pressing and fixing a film placed on the base plate. The locking mechanism is fixedly connected with each caliper fixing mechanism; each locking mechanism and the caliper fixing mechanism fixedly connected with the locking mechanism synchronously move along the sliding rail, and the sliding rail is locked after the locking mechanism and the caliper fixing mechanism stop moving. The auxiliary film cutting device is simple in structure, convenient to operate and capable of firmly fixing a film in the Western Blot experiment process, so that researchers can more efficiently, rapidly and accurately carry out auxiliary film cutting.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological experiment equipment technical field, concretely is a simple auxiliary film cutting device for western blot. BACKGROUND

[0002] Western Blot is protein blotting. Western Blot main steps include electrophoresis, transfer, blocking, hybridization and color development five, wherein protein electrophoresis separates after need to carry out the membrane transfer of SDS-PAGE gel, namely after the detection of the protein of purpose to PVDF film or NC film. The usual practice is according to the molecular weight size of the protein of purpose, cut the gel after the membrane transfer of SDS-PAGE gel. Based on this, relevant personnel have researched a variety of Western Blot film cutting device.

[0003] The existing western blot film cutting device usually fixes the cutter on the device, which often causes the cutting to be skewed due to the instability of the cutter; some use magnetic materials to fix the film, which is easy to damage the protein on the film during fixing and taking off. For example, the patent document CN219837882U discloses a "film cutting device for Western blot experiment", which uses a magnet to fix and the bottom plate needs to be made of metal material, which is easy to rust after long-term use; the cutting device is fixed on one side, which is easy to be skewed during use, and thus the film cutting is easy to be skewed. The patent application document CN116117915A discloses "a quick scissors for cutting Western blotting transfer film before and after transfer", the film cutting device is also fixed on the caliper in one direction, and the cutter is fixed by a connecting rod for cutting, which is also easy to be skewed. The patent document CN220719436U discloses "a dry and wet two Western blot experiment transfer film cutting device", the cutter is fixed on the slide rail, and the cutting position cannot be seen during cutting, which is easy to cause cutting failure. The patent document CN218462356U discloses "a flexible and convenient Western Blot film cutting device", the main feature is that the slide rail is fixed by high barriers on both sides, the caliper is easy to slip off during use, and the barriers on both sides are easy to hinder the operator during cutting, which is inconvenient to operate. The patent document CN218699233U discloses "a PVDF transfer film cutting device", which has similar problems with the patent document CN220719436U. The patent document CN221985291U discloses "a film cutting device for protein immunoblotting experiment", which has an integrated cutting and fixing design, but the fixing device is too wide and easy to damage the film surface, the cutter is a special cutter, which is difficult to replace, and the wheel type cutter is easy to be skewed to cause the film cutting to be skewed. The patent application document CN218778490U discloses "a film cutting device for protein immunoblotting experiment", which has a similar structure to the patent document CN221985291U and has the same problems.

[0004] The existing western blot film cutting device has some problems to a greater or lesser extent. The bottom plate of these devices is integrally formed with the base, and is easy to scratch after long-term cutting, which causes the film to be cut along the scratches and cannot guarantee the cutting regularity. The mechanical structure of some devices is too complex and is not easy to maintain and produce. Practical new type content

[0005] The utility model aims at providing a simple auxiliary film cutting device for Western Blot, to solve the problems that the existing device is easy to cause the cutting to be skewed and is easy to hinder the operator during film cutting.

[0006] The utility model is realized as follows:

[0007] The utility model provides a simple auxiliary cut film device for Western Blot, including the base, the middle part of base is provided with the backing plate, the both sides of backing plate on base are opened the strip recess, two slide rails are arranged in each strip recess, the both sides of each strip recess are respectively threaded with a caliper fixed mechanism, the both ends of caliper are respectively clamped on two caliper fixed mechanisms, and caliper is used for the compression fixed of film placed on the backing plate.

[0008] Preferably, a rectangular recess is opened in the middle part of the base, the backing plate is placed in the rectangular recess, and the backing plate protrudes from the surface of the base by 2-3 mm. The backing plate is replaceable.

[0009] Preferably, the backing plate is a light-colored ceramic backing plate. The backing plate in the utility model is a replaceable ceramic backing plate, which has the advantages of easy cleaning, wear resistance, corrosion resistance, replaceability, and anti-cut mark. The light color facilitates the identification of the mark position on the film and better determines the film cutting site.

[0010] Preferably, an inclination is provided at the lower end of the caliper in the form of an inclined slope structure. The caliper with a small inclination is used to fix the film on the backing plate, which can avoid damaging the film while fixing the film. The small inclination of the caliper can be additionally equipped with a rubber or silicone auxiliary protective sleeve for different use scenarios. The protective sleeve has the same shape as the small inclination of the caliper, so as to better protect the protein on the film from being damaged when fixing the film.

[0011] Preferably, the caliper is a steel caliper that is not easy to deform.

[0012] Preferably, the caliper fixing mechanism includes a caliper fixing seat, a gap for accommodating the caliper is opened at the opposite center positions of the two caliper fixing seats, the both ends of the caliper are clamped in the gaps of the two caliper fixing seats, and two small holes are provided at the both ends of the caliper for passing through locking pins. The caliper is fixed on the caliper fixing seat by the locking pins, which is convenient to operate and can be quickly disassembled. When the locking pin on one of the caliper fixing seats fixes the caliper, the other side can be adjusted in angle and height at will. This is suitable for use when the film size is small or continuous cutting of the film is needed, which facilitates the user to quickly contact the film and adjust the position of the film.

[0013] Preferably, a scale line is provided on the edge of the base corresponding to the strip recess where the slide rails are located, and a positioning pointer is provided on the bottom of the side of the caliper fixing seat facing the scale line. The positioning pointer can quickly correspond the caliper fixing mechanisms on both sides on the same horizontal straight line to ensure the flatness of the caliper.

[0014] Preferably, the device further comprises a locking mechanism fixedly connected with each caliper fixing mechanism; each locking mechanism moves along the slide rail synchronously with the caliper fixing mechanism fixedly connected therewith, and locks the slide rail when both stop moving; the locking mechanism comprises a hollow shell, a vertical column is connected to the top plate of the shell, the vertical column is in contact with an eccentric wheel above, and the vertical column is fixedly connected with a horizontal plate in the shell below, the eccentric wheel is fixed on a fixing frame on the shell through an eccentric shaft of the eccentric wheel; the horizontal plate is provided with clamping grooves at positions corresponding to the two slide rails on the bottom surface, and a return spring is arranged below the horizontal plate, one end of the return spring is fixedly connected to a bottom plate in the shell, and the other end abuts against the bottom of the horizontal plate.

[0015] Preferably, the return spring is sleeved on a fixing column fixed on the bottom plate in the shell, and the height of the fixing column is not higher than the height of the return spring after compression.

[0016] Preferably, a handle is fixedly connected to the eccentric wheel; the handle is pressed down, the eccentric wheel rotates and moves downward, the vertical column is pushed to move downward, the horizontal plate moves downward and the return spring is compressed, and the two clamping grooves on the horizontal plate are clamped on the two slide rails; the handle is lifted up, the eccentric wheel rotates and moves upward, the return spring pushes the horizontal plate and the vertical column to move upward, and the two clamping grooves on the horizontal plate are disengaged from the two slide rails.

[0017] Preferably, the caliper fixing seat and the bottom of the shell are both provided with rollers facilitating the movement of the caliper fixing seat and the shell along the slide rail.

[0018] Preferably, anti-skid supporting blocks are arranged below the base.

[0019] The simple auxiliary film cutting device for Western Blot can fix the film on the backing plate by using the caliper after determining the protein position, the small inclination angle of the lower end of the caliper can reduce the contact area of the caliper and the film while fixing the film, and the caliper can avoid scratching other positions of the film and damaging the target protein, especially when cutting the positions with similar protein sizes. Then, the film can be cut by using a scalpel or other cutters, which can provide more freedom for researchers and improve the film cutting accuracy and efficiency.

[0020] The device can conveniently assist in cutting the film, ensure controllability and convenience in the cutting process, solve the problems of the existing devices, such as the swinging of the cutter, the damage to the film surface by the fixing mode, the poor repeatability of the backing plate, and the overly complex structure, and enable researchers to more efficiently, quickly and accurately cut the film.

[0021] The device is simple in structure, convenient to use, easy to assemble and maintain, and convenient for large-scale production. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1It is a structural schematic view of the utility model.

[0023] Figure 2 It is to Figure 1 It is a sectional view after cutting of the locking mechanism.

[0024] Figure 3 It is Figure 1 It is a structural schematic view of the caliper; wherein, (a) is the front view of the caliper, (b) is the side view of the caliper.

[0025] Wherein: 1, base, 2, backing plate, 3, slide rail, 4, caliper, 4-1, small hole, 4-2, inclination, 5, caliper fixing mechanism, 5-1, caliper fixing seat, 5-2, strip-shaped through hole, 5-3, locking pin, 6, locking mechanism, 6-1, shell, 6-2, eccentric wheel, 6-3, handle, 6-4, stand, 6-5, cross plate, 6-6, return spring, 6-7, pin shaft, 7, alignment pointer, 8, roller. DETAILED DESCRIPTION

[0026] As Figure 1 And Figure 2 As shown in the utility model provides a simple auxiliary film cutting device for Western Blot, its structure includes base 1, and base 1 is flat plate structure;Rectangular recess is opened in the middle area on base 1, and replaceable backing plate 2 is placed in rectangular recess, and backing plate 2 is placed in rectangular recess after protruding on the surface of base 1 2-3mm. One strip-shaped recess is opened on the opposite side of rectangular recess on base 1, and two parallel slide rails 3 are arranged in each strip-shaped recess. Caliper fixing mechanism 5 and locking mechanism 6 are connected on slide rail 3, and caliper fixing mechanism 5 and locking mechanism 6 can move back and forth along slide rail 3.

[0027] Caliper fixing mechanism 5 is used for fixing caliper 4. Caliper fixing mechanism 5 specifically includes caliper fixing seat 5-1, and the caliper fixing seat 5-1 shown in the drawing is L-shaped seat body structure facing away from backing plate 2, and the bottom of caliper fixing seat 5-1 is provided with through hole corresponding to two slide rails 3 in strip-shaped recess, and caliper fixing seat 5-1 is connected on two slide rails 3 through the through hole thereon. Vertical slit for accommodating caliper 4 is opened in the middle part of caliper fixing seat 5-1, and the both ends of caliper 4 are respectively placed in the slit of caliper fixing seat 5-1 on both sides. Figure 3As shown. In order to fix the caliper 4, a vertical strip-shaped through hole 5-2 is also opened on the caliper fixing seat 5-1, and a small hole 4-1 is also opened on the caliper 4 at a position corresponding to the strip-shaped through hole 5-2, so that the locking pin 5-3 passes through the strip-shaped through hole 5-2 and the small hole 4-1 of the caliper 4, and cooperates with the nut to firmly fix the caliper 4 on the caliper fixing seat 5-1, thereby realizing the fixation of the caliper 4. Through the cooperation of the locking pin 5-3 and the nut, the caliper 4 can be quickly and conveniently fixed, and the height and inclination of the caliper can be conveniently adjusted, which is convenient for operation requirements and moving the position of the film.

[0028] The caliper 4 is used to press and fix the film on the backing plate 2, and plays a role in fixing the film and assisting in cutting the film to ensure that the incision is regular and straight. The material of the caliper 4 is preferably a steel material, and the caliper 4 is a sheet structure. In the utility model, the part of the caliper 4 in contact with the backing plate 2 is an inclined surface structure with an inclination angle 4-2. The inclination angle part is in contact with the film on the backing plate 2, which can reduce the contact area of the film and avoid damaging the film as much as possible. For different use scenarios, the small inclination angle surface of the caliper can be additionally provided with a rubber or silicone auxiliary protective sleeve, and the shape of the protective sleeve is consistent with the small inclination angle surface of the caliper, so as to better protect the protein on the film during the fixation of the film.

[0029] During the Western Blot experiment, first, one end of the caliper 4 is fixed on the caliper fixing seat 5-1, and after the position of the film is determined, the other end of the caliper 4 is fixed on the caliper fixing seat 5-1 on the other side, and the film is fixed and pressed on the backing plate 2 through the inclination angle 4-2 at the bottom of the caliper 4. Since the backing plate 2 protrudes from the base 1, the film thereon can better contact the caliper 4, thereby achieving a good fixing effect. Since the film is light-colored, in order to facilitate observation of the position of the protein on the film and to quickly find the cutting site, the backing plate 2 is also provided in a light color, such as white or gray. The backing plate 2 is made of wear-resistant light-colored ceramic material. The ceramic has good hydrophobicity, so it can be used in dry and wet cutting film scenes. The backing plate 2 in the utility model is easy to replace and convenient to use, and can solve the problem that the existing device is prone to scratches during use, thereby causing cutting difficulties.

[0030] The locking mechanism 6 includes a housing 6-1, which is a hollow cuboid structure. The two side plates of the housing 6-1 are fixedly connected with the caliper fixing seat 5-1 through two pin shafts 6-7, thereby realizing the fixed connection between the locking mechanism 6 and the caliper fixing mechanism 5. The two pin shafts 6-7 are respectively located outside the two slide rails 3. During normal operation, the locking mechanism 6 moves synchronously with the caliper fixing mechanism 5 along the slide rail 3 or stops moving at the same time, and the movement states of the two are synchronous. In case of failure, the locking mechanism 6 and the caliper fixing mechanism 5 can be easily separated by disassembling the pin shaft 6-7, thereby facilitating maintenance.

[0031] The locking mechanism 6 is used to lock the slide rail 3 when the locking mechanism 6 and the caliper fixing mechanism 5 stop moving, so that the locking mechanism 6 and the caliper fixing mechanism 5 no longer shake, the caliper 4 does not shake with the caliper fixing mechanism 5 after fixing the film, effectively ensuring that the position of the fixed film does not change, and ensuring that the incision is straight during cutting.

[0032] A stand 6-4 is connected at the center of the top plate of the shell 6-1, the upper end of the stand 6-4 is in contact with the eccentric wheel 6-2, and the two are in a hard friction relationship, in this embodiment, the upper end of the stand 6-4 is provided as a concave spherical surface structure, and the eccentric wheel 6-2 is located on the concave spherical surface; the eccentric wheel 6-2 is rotatably connected to the fixed frame through its eccentric shaft, and the fixed frame is fixedly arranged on the top plate of the shell 6-1. A handle 6-3 is fixedly connected above the eccentric wheel 6-2. The lower end of the stand 6-4 extends into the shell 6-1 and is fixedly connected with the transverse plate 6-5 in the shell 6-1. The bottom surface of the transverse plate 6-5 is provided with a clamping groove corresponding to the two slide rails 3, and a reset spring 6-6 is arranged at the lower middle part of the transverse plate 6-5, one end of the reset spring 6-6 is fixedly connected to the bottom plate in the shell 6-1, and the other end of the reset spring 6-6 abuts against the bottom of the transverse plate 6-5. The reset spring 6-6 can be sleeved on a fixed column, and the fixed column is fixed on the bottom plate in the shell 6-1, and the height of the fixed column is not higher than the height of the reset spring 6-6 after being compressed.

[0033] The handle 6-3 is lifted upward to drive the eccentric wheel 6-2 to rotate and displace upward, at this time the transverse plate 6-5 moves upward under the action of the elastic force of the reset spring 6-6, and the stand 6-4 moves upward and still contacts with the eccentric wheel 6-2; the handle 6-3 is pressed downward to drive the eccentric wheel 6-2 to rotate and displace downward, the eccentric wheel 6-2 moves downward to make the stand 6-4 move downward, and then the transverse plate 6-5 moves downward, and the reset spring 6-6 is compressed. After the transverse plate 6-5 moves downward, the two clamping grooves at the bottom of the transverse plate 6-5 are clamped on the two slide rails 3, and then the two clamping grooves at the bottom of the transverse plate 6-5 clamp and fix the two slide rails 3, so that the locking mechanism 6 and the caliper fixing mechanism 5 are fixed relative to the slide rails 3. When the locking mechanism 6 and the caliper fixing mechanism 5 need to move, the handle 6-3 is lifted upward to make the two clamping grooves of the transverse plate 6-5 disengage from the two slide rails 3, at this time the transverse plate 6-5 is still supported by the elastic force of the reset spring 6-6.

[0034] Rollers 8 are arranged at the bottom of the caliper fixing seat 5-1 and the shell 6-1, which play an auxiliary moving role, so that the caliper fixing mechanism 5 and the locking mechanism 6 can move smoothly on the slide rails 3.

[0035] As Figure 1As shown, the edge part corresponding to the strip-shaped recess where the slide rail 3 is located on the base 1 is provided with a scale line, and the bottom of the caliper fixing seat 5-1 on the side facing the scale line is provided with a positioning pointer 7, through which the caliper fixing mechanisms 5 on both sides can be quickly corresponded on the same horizontal straight line, so that the caliper 4 can be ensured to be kept straight.

[0036] Anti-skid supporting blocks can be arranged at the lower four corners of the base 1, and the material of the anti-skid supporting blocks can be silica gel, rubber or the like, so that the device of the utility model is applicable to various material tabletops and can prevent slipping during cutting.

Claims

1. A simple and easy-to-use membrane cutting device for Western Blot, characterized in that, The base is provided with a pad in the middle part, and a strip-shaped groove is formed on both sides of the pad on the base, two slide rails are arranged in each strip-shaped groove, a caliper fixing mechanism is respectively connected to the two slide rails in each strip-shaped groove, and the two ends of the caliper are respectively clamped on the two caliper fixing mechanisms, and the caliper is used for tightly fixing the film placed on the pad.

2. The simple membrane cutting aid for Western Blot according to claim 1, wherein A rectangular groove is formed in the middle part of the base, the pad is arranged in the rectangular groove, and the pad protrudes from the surface of the base by 2-3 mm, and the pad is replaceable.

3. The simple membrane cutting aid for Western Blot according to claim 2, characterized in that, The pad is a light-colored ceramic pad.

4. The simple membrane cutting device for Western Blot according to claim 1, wherein, An inclination is arranged at the lower end of the caliper in the form of an inclined slope structure.

5. The simple membrane cutting device for Western Blot according to claim 1, wherein, The caliper fixing mechanism comprises a caliper fixing seat, a gap for accommodating the caliper is formed at the opposite center positions of the two caliper fixing seats, the two ends of the caliper are clamped in the gaps of the two caliper fixing seats, and the caliper is fixed by a locking pin penetrating through the caliper fixing seat and the caliper.

6. The simple membrane cutting aid for Western Blot according to claim 5, wherein A scale line is arranged on the edge part of the base corresponding to the strip-shaped groove where the slide rail is arranged, an alignment pointer is arranged on the bottom of the side of the caliper fixing seat facing the scale line, and the two caliper fixing mechanisms on the two sides can be quickly aligned on the same horizontal straight line through the alignment pointer.

7. The simple membrane cutting device for Western Blot according to claim 5, wherein, Each locking mechanism is fixedly connected with the caliper fixing mechanism, each locking mechanism moves synchronously with the caliper fixing mechanism fixedly connected therewith along the slide rail, and the slide rail is locked when the two stop moving.

8. The simple membrane cutting device for Western Blot according to claim 7, characterized in that, The reset spring is sleeved on the fixing column, and the fixing column is fixed on the bottom plate in the shell. The height of the fixing column is not higher than the height of the reset spring after being compressed.

9. The simple membrane cutting device for Western Blot according to claim 7, wherein the membrane is cut by the cutter. The handle is fixedly connected to the eccentric wheel. The handle is pressed down, the eccentric wheel rotates and moves downward, the stand column is pushed to move downward, the horizontal plate moves downward and compresses the reset spring, and the two clamping grooves on the horizontal plate are clamped on the two slide rails. The handle is lifted up, the eccentric wheel rotates and moves upward, the reset spring pushes the horizontal plate and the stand column to move upward, and the two clamping grooves on the horizontal plate are separated from the two slide rails.

10. The simple membrane cutting device for Western Blot according to claim 7, wherein, Rollers are arranged on the bottom of the caliper fixing seat and the shell to facilitate the movement of the caliper fixing seat and the shell along the slide rail.

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