Stem cell culture device
By introducing an oscillation component into the stem cell culture device, the problem of stem cells adhering to the inner wall of the culture dish was solved, resulting in better culture outcomes.
Patent Information
- Application Number
- CN202423119736.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-17
- Publication Date
- 2025-11-28
- Estimated Expiration
- 2034-12-17
AI Technical Summary
In existing stem cell culture devices, stem cells tend to adhere to the inner wall of the culture dish, affecting growth.
A stem cell culture device including an oscillation component was designed. The oscillation motion of the placement plate is achieved by driving the active wheel and the driven wheel with a servo motor, which in turn drives the transmission rod, crank and guide wheel, thus preventing the stem cells from remaining static and attached for a long time.
This effectively prevents stem cells from remaining statically attached to the inner wall of the culture dish for extended periods, thus improving the stem cell culture effect.
Smart Images
Figure CN223607293U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to stem cell culture equipment field, specifically a stem cell culture device. BACKGROUND
[0002] The stem cell culture device is a kind of equipment specially used for stem cell culture and research, aims to provide a controllable platform simulating in-vivo environment to support the growth, proliferation and differentiation of stem cells, and is widely used in stem cell research, drug development and clinical treatment and other fields;
[0003] According to Chinese patent application No. 202222855818.4, a stem cell culture device is disclosed, comprising a culture box, the inner side wall of the culture box is fixedly connected with a honeycomb plate, the upper surface of the honeycomb plate is fixedly connected with a placing rack, the upper surface of the placing rack is fixedly connected with a plurality of culture dishes, the inner side wall of the culture box is fixedly connected with a connecting plate, the inner bottom wall of the culture box is fixedly connected with a heater, both output ends of the heater extend through the connecting plate and extend above the connecting plate, the upper surface of the connecting plate is fixedly connected with two support plates, the top end of each support plate is fixedly connected with the bottom surface of the honeycomb plate, the heater and the honeycomb plate cooperate to facilitate the discharge of heat to the lower side of the honeycomb plate, and the characteristics of the honeycomb plate are utilized to evenly disperse heat to the culture dishes, thereby achieving the purpose of heating the stem cells inside the culture dishes.
[0004] The prior art effectively solves the problem of reducing the internal temperature and affecting the growth of stem cells when the sealed door is opened to inject nutrient solution into the stem cells, and has the advantages of being able to heat the stem cells inside the culture dishes and improving the growth effect of stem cells, but the stem cell culture device can simulate the growth temperature of stem cells by heating when in use, and the stem cells cannot maintain a suspended state in the culture dishes, which easily adheres to the inner wall of the culture dishes, thereby affecting the growth of stem cells.
[0005] Therefore, the utility model provides a stem cell culture device to solve the above problems. UTILITY MODEL CONTENTS
[0006] To solve the above technical problems, the utility model provides the following technical scheme:
[0007] The utility model provides a stem cell culture device, including incubator, the incubator includes base, box, apron and the article plate, the inner chamber of base installs oscillation subassembly, the oscillation subassembly includes servo motor, driving wheel, driven wheel, crank, transmission rod, guide rod, guide pulley and connecting block, the oscillation subassembly is used to drive the article plate oscillation, the crank, transmission rod, guide rod, guide pulley and connecting block install in the inner chamber of base, the guide rod, guide pulley and connecting block are used for guiding the article plate, the servo motor, driving wheel and driven wheel install in the bottom of base, the surface of incubator is installed temperature control subassembly, and the temperature control subassembly is used for controlling culture temperature.
[0008] Further, in the utility model, the box is fixed on the top of the base, the article plate is installed in the inner chamber of the box, and the apron is hinged to the box through a hinge.
[0009] Further, in the utility model, the driving wheel is movably connected to the bottom of the base through a bearing, the servo motor is fixedly connected to the bottom of the base, and the driving wheel is drivingly connected to the driven wheel through a belt.
[0010] Further, in the utility model, one end of the transmission rod penetrates through the bottom of the base and is fixedly connected to the driven wheel, the other end of the transmission rod is fixedly connected to the crank, and the crank is movably connected to the article plate through a rotating shaft at the end away from the transmission rod.
[0011] Further, in the utility model, the guide rod is fixedly connected to the inner wall of the base, the guide pulley is located on the surface of the guide rod and is slidingly connected to the guide rod, one end of the connecting block is fixedly connected to the article plate, and the other end of the connecting block is movably connected to the guide pulley through a bearing.
[0012] Further, in the utility model, the temperature control subassembly comprises a mounting box, a controller, a heating rod, a fan, a temperature sensor, a flow guide groove and a dust screen, the mounting box is fixed to the back of the box, the temperature sensor is installed in the inner chamber of the box, the flow guide groove is formed in the back of the inner chamber of the box, and the dust screen is installed in the inner chamber of the flow guide groove.
[0013] Further, in the utility model, the heating rod and the fan are installed in the inner chamber of the mounting box, the controller is installed on the surface of the box, the output end of the temperature sensor is connected to the input end of the controller, and the output end of the controller is respectively connected to the input end of the heating rod, the fan and the servo motor.
[0014] Beneficial effects, the utility model has following beneficial effects:
[0015] The utility model discloses a base, box, apron, thing board and temperature control subassembly are set up, have provided the effect of can to stem cell provide culture space, through the cooperation of servo motor, driving wheel, driven wheel, crank, transmission rod, guide rod, guide pulley and connecting block, have provided the effect of can to stem cell inside petri dish oscillation, thereby can prevent stem cell long -time in stationary state affix in the inside wall of petri dish, effectively improved the culture effect of stem cell. BRIEF DESCRIPTION OF DRAWINGS
[0016] Figure 1 It is the main view structure schematic drawing of the utility model, and
[0017] Figure 2 It is the connection state structure schematic drawing of base and oscillation subassembly of the utility model,
[0018] Figure 3 It is the connection state structure schematic drawing of oscillation subassembly of the utility model,
[0019] Figure 4 It is the bottom view structure schematic drawing of base of the utility model,
[0020] Figure 5 It is the separation state structure schematic drawing of temperature control subassembly and box of the utility model.
[0021] In the drawing,
[0022] 1, incubator, 101, base, 102, box, 103, apron, 104, thing board, 2, oscillation subassembly, 201, servo motor, 202, driving wheel, 203, driven wheel, 204, crank, 205, transmission rod, 206, guide rod, 207, guide pulley, 208, connecting block, 3, temperature control subassembly, 301, installation box, 302, controller, 303, heating rod, 304, fan, 305, temperature sensor, 306, flow guide groove, 307, dustproof net. DETAILED DESCRIPTION
[0023] In order to understand the technical content of the utility model more, specific embodiment is raised and is explained as follows with the accompanying drawing. In the present disclosure, the aspects of the utility model are described with reference to the drawings, and many illustrated embodiments are shown in the drawings. The embodiments of the present disclosure are not necessarily defined in all aspects including the utility model. It should be understood that the various concepts and embodiments introduced above, and those concepts and embodiments described in more detail below can be implemented in any one of many ways, because the concepts and embodiments disclosed by the utility model are not limited to any implementation. In addition, some aspects of the utility model can be used alone, or used in any appropriate combination with other aspects of the utility model.
[0024] Embodiment 1
[0025] As Figures 1-5 shown, the first embodiment of the utility model provides a stem cell culture device, including incubator 1, incubator 1 includes base 101, box 102, cover plate 103 and the storage plate 104, the inner chamber of base 101 is installed with oscillation assembly 2, oscillation assembly 2 includes servo motor 201, driving wheel 202, driven wheel 203, crank 204, transmission rod 205, guide rod 206, guide wheel 207 and connecting block 208, oscillation assembly 2 is used to drive storage plate 104 oscillation, crank 204, transmission rod 205, guide rod 206, guide wheel 207 and connecting block 208 are installed in the inner chamber of base 101, guide rod 206, guide wheel 207 and connecting block 208 are used to guide storage plate 104, servo motor 201, driving wheel 202 and driven wheel 203 are installed at the bottom of base 101, the surface of incubator 1 is installed with temperature control assembly 3, and temperature control assembly 3 is used to control culture temperature.
[0026] As Figures 1-5 shown, through the cooperation of base 101, box 102, cover plate 103 and storage plate 104, culture space can be provided for stem cells, base 101, box 102 and cover plate 103 are used to provide a closed environment, and storage plate 104 is used to carry culture dishes, the inner chamber of box 102 can be heated by temperature control assembly 3, so that the culture temperature can be maintained, the output shaft of servo motor 201 rotates to drive driving wheel 202 to rotate, driving wheel 202 rotates to drive driven wheel 203 to rotate through a belt, driven wheel 203 rotates to drive transmission rod 205 to rotate, transmission rod 205 rotates to drive crank 204 to rotate, crank 204 rotates to push storage plate 104 to reciprocate left and right, and storage plate 104 moves to drive guide wheel 207 to reciprocate left and right along the surface of guide rod 206 through connecting block 208, so that the oscillation effect can be realized, and the stem cells can be prevented from being attached to the inner wall of the culture dish for a long time in a static state, and the culture effect of stem cells is effectively improved.
[0027] Embodiment 2
[0028] Referring to Figures 1-4 , the second embodiment of the utility model is based on the previous embodiment.
[0029] In this embodiment, box 102 is fixed to the top of base 101, storage plate 104 is installed in the inner chamber of box 102, and cover plate 103 is hinged to box 102 through a hinge.
[0030] Driving wheel 202 is movably connected to the bottom of base 101 through a bearing, servo motor 201 is fixedly connected to the bottom of base 101, and driving wheel 202 is drivingly connected to driven wheel 203 through a belt.
[0031] One end of the transmission rod 205 penetrates to the bottom of the base 101 and is fixedly connected with the driven wheel 203, and the other end of the transmission rod 205 is fixedly connected with the crank 204, and the end of the crank 204 away from the transmission rod 205 is movably connected with the storage plate 104 through a rotating shaft.
[0032] The guide rod 206 is fixedly connected with the inner wall of the base 101, the guide wheel 207 is located on the surface of the guide rod 206 and is movably connected with the guide rod 206, one end of the connecting block 208 is fixedly connected with the storage plate 104, and the other end of the connecting block 208 is movably connected with the guide wheel 207 through a bearing.
[0033] As shown in Figures 1-4 , the transmission rod 205 is connected with the base 101 through a bearing, the servo motor 201 can be connected with the base 101 through bolts, and the servo motor 201 can also be installed in the inner cavity of the base 101, when the driving wheel 202 rotates, the driven wheel 203 is driven to rotate through the belt, when the driven wheel 203 rotates, the transmission rod 205 is driven to rotate, when the transmission rod 205 rotates, the crank 204 is driven to rotate, when the crank 204 rotates, the storage plate 104 is pushed to move left and right reciprocatingly, when the storage plate 104 moves, the guide wheel 207 is driven to move left and right reciprocatingly along the surface of the guide rod 206 through the connecting block 208, so that the oscillation effect can be realized.
[0034] Embodiment 3
[0035] Referring to Figure 1 and 5 , this is the third embodiment of the utility model, and the embodiment is based on the previous two embodiments.
[0036] In the embodiment, the temperature control assembly 3 comprises a mounting box 301, a controller 302, a heating rod 303, a fan 304, a temperature sensor 305, a guide groove 306 and a dustproof net 307, the mounting box 301 is fixed to the back of the box body 102, the temperature sensor 305 is installed in the inner cavity of the box body 102, the guide groove 306 is arranged on the back of the inner cavity of the box body 102, and the dustproof net 307 is installed in the inner cavity of the guide groove 306.
[0037] The heating rod 303 and the fan 304 are both installed in the inner cavity of the mounting box 301, the controller 302 is installed on the surface of the box body 102, the output end of the temperature sensor 305 is connected with the input end of the controller 302, and the output end of the controller 302 is connected with the input end of the heating rod 303, the fan 304 and the servo motor 201 respectively.
[0038] As shown in Figure 1 and 5As shown, by the controller 302 can not only set the heating temperature, but also can set the speed of servo motor 201, so as to adjust the oscillation frequency, the heat generated by the heating rod 303 is blown to the inner cavity of the box 102 by the fan 304, so as to keep the inner cavity of the box 102 heat, the flow guide groove 306 and the dust screen 307 are used for heat conduction.
[0039] In use, first, the culture dish is placed on the top of the placing plate 104, and then the cover plate 103 is covered, the inner cavity of the box 102 is heated by the temperature control assembly 3, so that the culture temperature can be maintained, the output shaft of the servo motor 201 rotates to drive the driving wheel 202 to rotate, the driving wheel 202 rotates to drive the driven wheel 203 to rotate through the belt, the driven wheel 203 rotates to drive the transmission rod 205 to rotate, the transmission rod 205 rotates to drive the crank 204 to rotate, the crank 204 rotates to push the placing plate 104 to reciprocate left and right, the placing plate 104 moves to drive the guide wheel 207 to reciprocate left and right along the surface of the guide rod 206 through the connecting block 208, so that the oscillation effect can be realized, thereby preventing the stem cells from being attached to the inner wall of the culture dish for a long time in a static state, and effectively improving the culture effect of the stem cells.
[0040] The standard parts used in the present application can be purchased from the market, and can be customized according to the description and drawings, and the specific connection mode of each part adopts the conventional means such as bolt, rivet and welding in the prior art, the mechanical, parts and equipment adopt the conventional type in the prior art, the control mode is automatically controlled by the controller, and the control circuit of the controller can be realized by simple programming of the person skilled in the art, which belongs to the common knowledge in the art, and the present application is mainly used to protect the mechanical device, so the control mode and circuit connection are not explained in detail.
[0041] Although the present application has been disclosed with the preferred embodiments, it is not intended to limit the present application. Those skilled in the art can make various modifications and decorations without departing from the spirit and scope of the present application. Therefore, the protection scope of the present application is defined by the claims.
Claims
1. A stem cell culture device comprising a culture cabinet (1), characterized in that: The incubator (1) comprises a base (101), a box body (102), a cover plate (103) and a storage plate (104), an oscillation assembly (2) is installed in the inner cavity of the base (101), the oscillation assembly (2) comprises a servo motor (201), a driving wheel (202), a driven wheel (203), a crank (204), a transmission rod (205), a guide rod (206), a guide wheel (207) and a connecting block (208), the oscillation assembly (2) is used for driving the storage plate (104) to oscillate, the crank (204), the transmission rod (205), the guide rod (206), the guide wheel (207) and the connecting block (208) are installed in the inner cavity of the base (101), the guide rod (206), the guide wheel (207) and the connecting block (208) are used for guiding the storage plate (104), the servo motor (201), the driving wheel (202) and the driven wheel (203) are installed at the bottom of the base (101), and a temperature control assembly (3) is installed on the surface of the incubator (1), and the temperature control assembly (3) is used for controlling the culture temperature.
2. The stem cell culture device of claim 1, wherein: The box body (102) is fixed to the top of the base (101), the storage plate (104) is installed in the inner cavity of the box body (102), and the cover plate (103) is hinged to the box body (102) through a hinge.
3. The stem cell culture device of claim 1, wherein: The driving wheel (202) is movably connected to the bottom of the base (101) through a bearing, the servo motor (201) is fixedly connected to the bottom of the base (101), and the driving wheel (202) is drivingly connected to the driven wheel (203) through a belt.
4. The stem cell culture device of claim 1, wherein: One end of the transmission rod (205) penetrates to the bottom of the base (101) and is fixedly connected to the driven wheel (203), the other end of the transmission rod (205) is fixedly connected to the crank (204), and the crank (204) is movably connected to the storage plate (104) through a rotating shaft away from the transmission rod (205).
5. The stem cell culture device of claim 1, wherein: The guide rod (206) is fixedly connected to the inner wall of the base (101), the guide wheel (207) is located on the surface of the guide rod (206) and is slidingly connected to the guide rod (206), one end of the connecting block (208) is fixedly connected to the storage plate (104), and the other end of the connecting block (208) is movably connected to the guide wheel (207) through a bearing.
6. The stem cell culture device of claim 1, wherein: The temperature control assembly (3) comprises a mounting box (301), a controller (302), a heating rod (303), a fan (304), a temperature sensor (305), a flow guide groove (306) and a dustproof net (307), the mounting box (301) is fixed to the back of the box body (102), the temperature sensor (305) is installed in the inner cavity of the box body (102), the flow guide groove (306) is formed in the back of the inner cavity of the box body (102), and the dustproof net (307) is installed in the inner cavity of the flow guide groove (306).
7. The stem cell culture device of claim 6, wherein: The heating rod (303) and the fan (304) are installed in the inner cavity of the installation box (301), the controller (302) is installed on the surface of the box (102), the output end of the temperature sensor (305) is connected with the input end of the controller (302), and the output end of the controller (302) is connected with the input end of the heating rod (303), the fan (304) and the servo motor (201) respectively.