Cell solution sampler and sampling equipment

Abstract

The utility model relates to the technical field of medical instruments, and provides a cell solution sampler and sampling equipment, the cell solution sampler comprises a first joint, a sampling container and a second joint which are sequentially connected through a pipeline; the connector assembly is arranged between the first connector and the sampling container; the connector assembly comprises a normally-closed connector and a male Luer connector which are detachably connected. When the normally-closed connector is separated from the male Luer connector, the normally-closed connector is in a closed state; and the first liquid stopping clamp is arranged on the pipeline between the joint assembly and the second joint. According to the cell solution sampler provided by the utility model, in actual use, the normally-closed joint can be separated from the male Luer joint in a rotating manner, and at the moment, the normally-closed joint is in a closed state, so that the first joint is closed; moreover, due to the existence of the first liquid stopping clamp, the sampling container can be closed, and then the combination of the male Luer connector, the first liquid stopping clamp and the sampling container can be taken away for sampling operation.

Description

TECHNICAL FIELD

[0001] The utility model relates to medical instrument technical field, concretely relates to a cell solution sampler and sampling equipment. BACKGROUND

[0002] Cell therapy is a new disease treatment method, it utilizes the characteristics of the cell with specific function, obtains and / or carries out in vitro amplification, special culture etc. processing through bioengineering means, makes these cells possess the treatment efficacy such as enhancing immunity, killing pathogen and tumor cell, promoting tissue organ regeneration and organism rehabilitation to achieve the purpose of treating diseases. Cell therapy needs a large number of cells, the most common is to use cell culture bag, cell culture bottle etc. cell large-scale culture container, in vitro simulation cell in vivo growth environment to realize cell growth, propagation and differentiation to obtain. Large-scale cell culture ware is usually equipped with special interface, convenient for adding culture medium, drug etc. reagent, also convenient for cell harvesting and transfer. Prior art discloses a cell solution sampler, including first joint, pipe clamp, sampling container, second joint and filter, one end of first joint is used for connecting external cell container, the other end of first joint is communicated with sampling container, one end of second joint connects filter, the other end of second joint is communicated with the end of sampling container away from first joint;The end of filter away from second joint is used for external liquid aspirator;Pipe clamp is clamped on the pipeline between first joint and sampling container. When using, external liquid aspirator extracts the cell solution sampler into negative pressure state, and the cell solution sampler in negative pressure state extracts the cell liquid in external cell container into sampling container, when the capacity of cell solution in sampling container reaches the preset volume (that is, the volume of the cell solution needed to be sampled), external liquid aspirator stops sampling. Then the pipe clamp clamps the pipeline between sampling container and first joint, and the pipeline is sealed by using heat sealing process etc. to separate first joint and sampling container;The separated sampling container is communicated with second joint, and the sampling container is in closed state. Then, the pipeline at the end of sampling container away from second joint is cut, and the mouth of the pipeline is directed to external ware (centrifugal cup, culture dish, test tube etc.), and the pipe clamp is opened. Finally, external liquid aspirator injects gas into sampling container, and the gas flows into external ware from sampling container, thereby completing the sampling work of cell solution;And when external liquid aspirator fills gas into sampling container, the gas is filtered through filter, thereby further avoiding the pollution of gas to cell solution, and ensuring the cleanliness of cell solution. However, the cell solution sampler needs to use heat sealing forceps and scissors when using, the heat sealing forceps is expensive, and the requirement for operator is higher. SUMMARY

[0003] Therefore, the technical problem to be solved by the utility model lies in that the cell solution sampler in the prior art needs to use heat sealing forceps and scissors, the heat sealing forceps are expensive and have high requirements on the operator, thereby providing a cell solution sampler and a sampling device.

[0004] To solve the above technical problems, the technical scheme of the utility model is as follows:

[0005] On the one hand, the utility model provides a kind of cell solution sampler, including first joint, sampling container and second joint connected by pipeline in sequence;Further include: joint assembly, between the first joint and sampling container;The joint assembly includes the normally closed joint and luer male joint of detachable connection;When the normally closed joint and the luer male joint are separated, the normally closed joint is in closed state;When the normally closed joint and the luer male joint are connected, the normally closed joint is in open state;First liquid stop clamp, on the pipeline between the joint assembly and the second joint.

[0006] Further, the cell solution sampler further includes a closed luer female joint, and the closed luer female joint is connected with the luer male joint after the normally closed joint is separated from the luer male joint.

[0007] Further, the cell solution sampler further includes a second liquid stop clamp arranged on the pipeline between the joint assembly and the first joint.

[0008] Further, the cell solution sampler further includes a filter arranged on the pipeline away from the sampling container on the side of the second joint.

[0009] Further, the cell solution sampler further includes a first cap detachably covering one end of the filter away from the sampling container.

[0010] Further, the cell solution sampler further includes a second cap detachably covering one end of the first joint away from the sampling container.

[0011] Further, the cell solution sampler further includes a tube joint, and the tube joint has a plurality of branch interfaces, the tube joint is connected with the first joint in communication, and each branch interface is adapted to one normally closed joint.

[0012] On the other hand, the utility model further provides a sampling device including the above-mentioned cell solution sampler.

[0013] Further, the sampling device further includes a liquid extractor connected with one end of the filter away from the second joint.

[0014] Further, the sampling device further comprises a sterile cotton swab, which wipes the luer male joint after the normally closed joint is separated from the luer male joint.

[0015] The technical scheme of the utility model has the following advantages:

[0016] The cell solution sampler provided by the utility model, in actual use, if no operator skilled in using the heat sealing pliers and scissors is equipped, or the sampler is fixed in a device with insufficient operation space such as a cell culture box, the normally closed joint can be separated from the luer male joint in a rotating mode, at this time, the normally closed joint is in a closed state, the closure of the pipeline end on one side of the first joint is realized, and moreover, due to the existence of the first liquid stop clamp, the closure of the pipeline end on one side of the sampling container is also realized, and then the combination of the luer male joint, the first liquid stop clamp and the sampling container can be taken away for sampling operation. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the specific embodiments of the utility model or the technical scheme in the prior art, the drawings needed to be used in the specific embodiments or the prior art description will be briefly introduced as follows, and obviously, the drawings in the following description are some embodiments of the utility model, and for those skilled in the art, other drawings can also be obtained according to these drawings without creative labor.

[0018] Figure 1 It is a schematic view of the cell solution sampler in an embodiment of the utility model;

[0019] Figure 2 It is a schematic view of the cell solution sampler in an embodiment of the utility model in a use scenario;

[0020] Figure 3 It is a schematic view of the cell solution sampler in an embodiment of the utility model in another use scenario (after heat sealing);

[0021] Figure 4 It is a schematic view of the cell solution sampler in an embodiment of the utility model in another use scenario (after cutting);

[0022] Figure 5 It is a schematic view of the cell solution sampler in another embodiment of the utility model;

[0023] Figure 6 It is a schematic view of the cell solution sampler in another embodiment of the utility model.

[0024] EXPLANATION OF REFERENCE NUMERALS:

[0025] 1. first connector; 2. sample container; 3. second connector; 4. normally closed connector; 5. male luer; 6. first liquid stopper; 7. second liquid stopper; 8. filter; 9. pipe joint. DETAILED DESCRIPTION

[0026] The technical solutions of the present application will be described clearly and completely below with reference to the drawings. Obviously, the described embodiments are only some of the embodiments of the present application, rather than all the embodiments. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of the present application.

[0027] In the description of the present application, it should be noted that the orientations or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application. In addition, the terms "first", "second", "third" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.

[0028] In the description of the present application, it should be noted that unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connecting" should be understood broadly, for example, it can be fixed connection, or detachable connection, or integrally connected; it can be mechanical connection, or electrical connection; it can be directly connected, or indirectly connected through an intermediate medium, or the communication between two elements. For those skilled in the art, the specific meanings of the above terms in the present application can be understood according to the specific circumstances.

[0029] In addition, the technical features involved in the different embodiments of the present application described below can be combined with each other as long as there is no conflict.

[0030] As Figure 1 , Figure 2As shown, the embodiment provides a cell solution sampler, which comprises a first connector 1, a sampling container 2 and a second connector 3 connected in sequence through pipelines; further comprises: a connector assembly arranged between the first connector 1 and the sampling container 2; the connector assembly comprises a normally closed connector 4 and a luer male connector 5 connected detachably; when the normally closed connector 4 is separated from the luer male connector 5, the normally closed connector 4 is in a closed state; when the normally closed connector 4 is connected with the luer male connector 5, the normally closed connector 4 is in an open state; a first liquid stop clamp 6 is arranged on the pipeline between the connector assembly and the second connector 3. Wherein, the normally closed connector 4 can adopt an existing normally closed connector 4, which is internally provided with an elastic valve core; when the luer male connector 5 is connected with the normally closed connector 4, the valve core is compressed inward, and the opening at the end of the valve core is open, at this time the normally closed connector 4 is in an open state. When the luer male connector 5 is separated from the normally closed connector 4, the valve core expands outward without external force and returns to the initial state, and the opening at the end of the valve core is closed, at this time the normally closed connector 4 is in a closed state. Of course, the connector assembly here can also be replaced with other valve or connector structures as long as similar functions are achieved.

[0031] In actual use, if there is no operator skilled in using heat sealing pliers and scissors, or the sampler is fixed in a device with insufficient operation space such as a cell incubator, the normally closed connector 4 can be separated from the luer male connector 5 by rotating, at this time the normally closed connector 4 is in a closed state, realizing the closure of the pipeline end on the side of the first connector 1; moreover, due to the existence of the first liquid stop clamp 6, the closure of the pipeline end on the side of the sampling container 2 can also be realized, and then the combination of the luer male connector 5, the first liquid stop clamp 6 and the sampling container 2 can be taken away for sampling operation.

[0032] Wherein, the cell solution sampler further comprises a closed luer female connector connected with the luer male connector 5 after the normally closed connector 4 is separated from the luer male connector 5. In this way, the closure of the luer male connector 5 can be realized.

[0033] Wherein, the cell solution sampler further comprises a second liquid stop clamp 7 arranged on the pipeline between the connector assembly and the first connector 1. In this way, the second liquid stop clamp 7 can limit the flow of liquid in the cell incubator to the normally closed connector 4 through the first connector 1.

[0034] Wherein, the cell solution sampler further comprises a filter 8 arranged on the pipeline on the side of the second connector 3 away from the sampling container 2. In this way, when the sampling container 2 is filled with gas through an external liquid extractor, the gas can be filtered, thereby avoiding the pollution of the gas to the cell solution and ensuring the cleanliness of the cell solution.

[0035] The cell solution sampler further comprises a first cap which is detachably coupled to the filter 8 at the end away from the sampling container 2. For example, the first cap and the filter 8 can be coupled by screw threads.

[0036] The cell solution sampler further comprises a second cap which is detachably coupled to the first joint 1 at the end away from the sampling container 2. For example, the second cap and the first joint 1 can be coupled by screw threads.

[0037] As shown in Figure 5 , Figure 6 The cell solution sampler further comprises a tube joint 9 which has a plurality of branch interfaces; the tube joint 9 and the first joint 1 are connected by a tube; each branch interface is adapted to a normally closed joint 4, and the normally closed joint 4 is adapted to a luer male joint 5, a first liquid stopper 6 and the sampling container 2 according to the requirement during use. For example, the tube joint 9 can have three branch interfaces, and the cell solution sampler is designed as a triple cell solution sampler. For another example, two tube joints 9 each having three branch interfaces can be used simultaneously, and the cell solution sampler is designed as a sextuple cell solution sampler. In this way, the cell culture device can be connected to a plurality of cell solution samplers simultaneously, and the multiple sampling can be facilitated.

[0038] Another embodiment provides a sampling device which comprises the above-mentioned cell solution sampler.

[0039] The sampling device further comprises a liquid aspirator which is connected to the filter 8 at the end away from the second joint 3. During use, the liquid aspirator is used to inject gas into the sampling container 2, and the gas causes the cell solution in the sampling container 2 to flow into the external container, thereby completing the sampling of the cell solution.

[0040] The sampling device further comprises a sterile cotton swab which is used to wipe the luer male joint 5 after the luer male joint 5 is separated from the luer female joint. In this way, the residual liquid at the interface of the luer male joint 5 can be wiped clean, and the cleanliness of the connection between the luer male joint 5 and the luer female joint can be maintained.

[0041] During use:

[0042] As shown in Figure 3 , Figure 4As shown, if equipped with heat-sealing forceps and scissors, and with sufficient operating space and skilled personnel, a conventional method can be used. After drawing the required cell solution into the sampling container 2, first close the first stop clamp 6 and the second stop clamp 7. Then, heat-seal the Luer male connector 5 between it and the first stop clamp 6 and cut it. Next, cut the tubing at the end of the sampling container 2 away from the second connector 3 and open the first stop clamp 6. Finally, use a pump to inject gas into the sampling container 2. The gas will cause the cell solution in the sampling container 2 to flow out, completing the cell solution sampling. At this time, the Luer male connector 5 is still connected to the side where the normally closed connector 4 is located. Rotate the Luer male connector 5 off the normally closed connector 4 and connect a new combination of Luer male connector 5, first stop clamp 6, and sampling container 2. Sampling operations can then be performed again. The first connector 1, the second stop clamp 7, and the normally closed connector 4 remain sterile throughout, making them suitable for long-term use.

[0043] like Figure 2 As shown, if there are no skilled operators proficient in using heat-sealing forceps and scissors, or if the sampler is fixed in equipment with insufficient operating space, such as a cell culture incubator, the normally closed connector 4 and the Luer male connector 5 can be separated by rotation. The normally closed connector 4 will then return to its normally closed state. After wiping the Luer male connector 5 with a sterile cotton swab to remove any residual droplets from the connector opening, a sealed Luer female connector can be screwed onto the Luer male connector 5. The combination of the Luer male connector 5, the first stop clamp 6, and the sampling container 2 can then be removed for sampling. In this scenario, heat-sealing forceps and scissors are unnecessary; only routine sterile cotton swab wiping is required.

[0044] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the protection scope of this invention.

Claims

1. A cell solution sampler comprising a first connector (1), a sampling container (2) and a second connector (3) connected in series by a pipe; characterized in that, Also comprising: a joint assembly disposed between the first joint (1) and the sampling container (2); the joint assembly comprises a detachable connection of a normally closed joint (4) and a luer male joint (5); when the normally closed joint (4) is separated from the luer male joint (5), the normally closed joint (4) is in a closed state; when the normally closed joint (4) is connected with the luer male joint (5), the normally closed joint (4) is in an open state; a first liquid stopper (6) disposed on the pipeline between the joint assembly and the second joint (3).

2. The cell solution sampler according to claim 1, wherein further comprising a closed luer female joint, which is connected with the luer male joint (5) after the normally closed joint (4) is separated from the luer male joint (5).

3. The cell solution sampler according to claim 1, wherein further comprising a second liquid stopper (7) disposed on the pipeline between the joint assembly and the first joint (1).

4. The cell solution sampler according to claim 1, wherein further comprising a filter (8) disposed on the pipeline on the side of the second joint (3) away from the sampling container (2).

5. The cell solution sampler according to claim 4, wherein further comprising a first cap detachably covering the end of the filter (8) away from the sampling container (2).

6. The cell solution sampler according to claim 1, wherein further comprising a second cap detachably covering the end of the first joint (1) away from the sampling container (2).

7. The cell solution sampler according to claim 1, wherein further comprising a tube joint (9) having a plurality of branch interfaces, the tube joint (9) being connected with the first joint (1), and each of the branch interfaces being adapted to accommodate one of the normally closed joints (4).

8. A sampling device, characterized by The cell solution sampler according to any one of claims 1 to 7.

9. The sampling device according to claim 8, wherein further comprising a liquid extractor connected with the end of the filter (8) away from the second joint (3).

10. The sampling device of claim 8, wherein, further comprising a sterile cotton swab for wiping the luer male joint (5) after the normally closed joint (4) is separated from the luer male joint (5).

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