CTC enriching and dyeing integrated equipment

Abstract

The utility model relates to the technical field of medical inspection equipment, in particular to CTC enrichment and dyeing integrated equipment. Comprising a horizontal conveying device for horizontally moving the test tube rack, an overturning platform for overturning the test tube rack, and a sliding rail platform for mounting a micro-fluidic chip. The equipment is high in automation degree, high in cell enrichment and dyeing operation efficiency and particularly suitable for cell enrichment and dyeing operation of batch CTC chips, and the CTC detection efficiency is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to medical examination equipment technical field, concretely is a CTC enrichment dyeing integrated equipment. BACKGROUND

[0002] CTC is circulating tumor cell detection, and it is a new tumor diagnosis and detection technology in recent years. According to research, circulating tumor cells can be found in peripheral blood before the formation of solid tumor, so CTC detection is very suitable for early screening and early diagnosis of malignant tumor, and CTC detection has very good effect on prognosis of malignant tumor, disease progression monitoring, recurrence prediction, postoperative microlesion monitoring of malignant tumor and design and treatment effect monitoring of targeted drug treatment, and it is an advanced method for early screening and diagnosis of malignant tumor. But due to the small amount of circulating tumor cells in peripheral blood, the capture and identification of circulating tumor cells in CTC detection are great challenges. The other Chinese patent 2019103326886 of the applicant discloses a nucleated cell suction printing enrichment and suction dyeing integrated reaction device and method in body fluid, and the authorization announcement number is CN111855333B. The reaction device includes a microporous membrane, the microporous membrane is provided with a fixing piece, the fixing piece is provided with a reaction tank, an upper cover is arranged on the reaction tank, a lifting object table is arranged on one side of the fixing piece, and a storage bin is arranged below the fixing piece. The storage bin is filled with water-absorbing material. When cell suspension passes through the microporous membrane, the cells larger than the membrane pore size are retained in the reaction container by adopting suction filtration retention, and are adsorbed on the membrane surface. The water-absorbing material has stable suction speed, soft and slow suction force and basically no pressure, so that the cells adhere to the surface of the membrane. The capture and enrichment of malignant cells and pathogenic organisms in the body fluid are realized. The microporous membrane in the device is also called a microfluidic chip, which is a key component for enriching circulating tumor cells. The existing microporous membrane adopts a filter membrane or filter paper made of flexible fiber material as a filter material, for example, the other Chinese patent 202321916277X of the applicant discloses a microfluidic device and cell enrichment dyeing integrated equipment, and the authorization announcement number is CN220371064U. The microfluidic device includes a liquid accumulation base table, a filter assembly and a base table net. The liquid accumulation base table is in a funnel-shaped structure, is provided with a flow guide strip protruding from the surface of the funnel-shaped structure and is used for guiding the filtered liquid; and the filter assembly is a microfilter membrane arranged above the liquid accumulation base table and is used for filtering liquid samples. In the CTC detection process, blood samples and test reagents need to be added dropwise to the microfluidic device one by one, and the operation is complicated and the work efficiency is low. UTILITY MODEL CONTENT

[0003] In order to solve the problems of the prior art, the utility model provides a CTC enrichment dyeing integrated equipment for cell enrichment dyeing operation of batch CTC chips, and improves the CTC detection efficiency.

[0004] In order to achieve the above object, the utility model provides the following technical scheme:

[0005] The utility model discloses a CTC enrichment dyeing integrated equipment, including horizontal conveying device for horizontal movement test tube rack, the turnover platform for overturning test tube rack, the slide rail platform for installing micro -fluidic chip, turnover platform sets up in the terminal of horizontal conveying device, the chip subrack of rectangular frame structure for installing micro -fluidic chip is slidably installed on the slide rail platform, and the chip body for CTC enrichment dyeing is provided on the micro -fluidic chip.

[0006] Adopt the structure design, and this equipment sets reagent filling, enrichment dyeing and other functions of CTC chip as a whole, can carry out enrichment dyeing to a plurality of micro -fluidic chips simultaneously, improves CTC detection efficiency. It can be used for batch CTC chip cell enrichment dyeing operation.

[0007] Preferably, the turnover platform includes a rotating shaft disposed at the terminal of the horizontal conveying device, a servo motor disposed at one end of the rotating shaft for driving the rotating shaft to rotate, and a test tube holder fixedly connected to the rotating shaft for receiving the test tube rack. The servo motor is fixedly installed on a sliding block, and the sliding block is slidably installed on a linear slide rail.

[0008] With the above structure design, the automatic operation of the test tube rack dumping is facilitated, and the workload of personnel is reduced.

[0009] Preferably, the slide rail platform includes left and right slide grooves arranged in parallel and capable of being lifted simultaneously. A heating platform is arranged in the gap between the left and right slide grooves, and a second lifting device is arranged on the outer side of the heating platform.

[0010] With the above structure design, the micro -fluidic chip is heated by the heating platform, and the reaction activity of the dyeing reagent is enhanced.

[0011] Preferably, the two linear slide rails are symmetrically arranged at the two ends of the rotating shaft, and the two ends of the rotating shaft are slidably installed on the linear slide rails through the sliding blocks. The left and right slide grooves are located on the inner sides of the two linear slide rails and are parallel to the linear slide rails. First lifting devices are arranged at the lower ends of the left and right slide grooves for controlling the lifting of the left and right slide grooves.

[0012] With the above structure design, the rotating shaft is balanced in stress and runs smoothly. The left and right slide grooves can be lifted, improving the level of automation operation.

[0013] Preferably, the horizontal conveying device is a belt conveyor.

[0014] With the above structure design, the test tube rack is conveyed to the test tube holder by the belt conveyor.

[0015] Preferably, a recycling mechanism is arranged below the rotation track, and the recycling mechanism is a belt conveyor with a conveying direction opposite to the horizontal conveying device.

[0016] With the above structure, the recycling mechanism can recycle the test tubes after reagent filling.

[0017] Preferably, the chip assembly bracket of the rectangular frame structure is slidably arranged on the left sliding groove and the right sliding groove, four corners of the chip assembly bracket are provided with pulleys through extension rods perpendicular to the left sliding groove or the right sliding groove, the pulleys are arranged in the left sliding groove or the right sliding groove, and the second jacking device is used for jacking the extension rods.

[0018] With the above structure, horizontal movement and tilting action of the chip assembly bracket can be realized.

[0019] Preferably, a bearing platform for clamping the microfluidic chip is arranged in the frame of the chip assembly bracket, and the bearing platform is a folded edge arranged on the inner side of the frame of the chip assembly bracket.

[0020] With the above structure, the microfluidic chip can be conveniently arranged on the chip assembly bracket, and the microfluidic chip can be conveniently taken and placed.

[0021] Compared with the prior art, the device has the advantages that:

[0022] The device has high automation degree and high operation efficiency of cell enrichment and staining, and is especially suitable for cell enrichment and staining operation of batch CTC chips, and improves CTC detection efficiency. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 It is a perspective structural schematic view of an embodiment of the device.

[0024] Figure 2 It is a top view structural schematic view of the linear slide rail.

[0025] Figure 3 It is a perspective structural schematic view of the chip assembly bracket.

[0026] Figure 4 It is a sectional view structural schematic view of the microfluidic chip.

[0027] Figure 5 It is a perspective structural schematic view of the chip fixing member of the microfluidic chip.

[0028] Figure 6 It is a sectional view structural schematic view of the chip fixing member.

[0029] Figure 7 It is a perspective structural schematic view of the chip body.

[0030] Figure 8 is a schematic diagram of a three-dimensional structure of the liquid absorption sheet.

[0031] Figure 9 is a schematic diagram of a three-dimensional structure of the liquid absorption assembly. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0033] As shown in Figure 1 , the CTC enrichment and staining device comprises a horizontal conveying device 1 for horizontally moving a test tube rack 8, a turnover platform for overturning the test tube rack 8, and a slide rail platform 5 for mounting a microfluidic chip 7, wherein

[0034] The horizontal conveying device 1 is a belt conveyor, and the test tube rack 8 carrying test tubes is placed on the belt conveyor in use, and the test tube rack 8 is conveyed one by one to the turnover platform by the belt conveyor.

[0035] The turnover platform comprises a rotating shaft 2 arranged at the terminal end of the horizontal conveying device 1, a servo motor 21 arranged at one end of the rotating shaft 2 and used for driving the rotating shaft 2 to rotate, and a test tube bracket 22 fixedly connected to the rotating shaft 2, wherein the servo motor 21 is fixedly installed on a sliding block, and the sliding block is slidingly installed on a linear slide rail 10.

[0036] The test tube bracket 22 is a folded plate with an L-shaped cross section, and the bottom plate of the test tube bracket 22 is in a horizontal state and close to the terminal end of the horizontal conveying device 1 in the initial state. When the test tube rack 8 provided with test tubes is moved to the terminal end of the horizontal conveying device 1, it can be smoothly transferred to the bottom plate of the test tube bracket 22.

[0037] As shown in Figure 1 , Figure 2 , the servo motor 21 is fixedly installed on a sliding block, and the sliding block is slidingly installed on a linear slide rail 10, which comprises two tracks arranged at two ends of the rotating shaft 2, respectively, and the two ends of the rotating shaft 2 are installed on the two tracks through the sliding blocks, and at least one of the sliding blocks is provided with the servo motor 21 for driving the rotating shaft 2 to rotate; in addition, the linear slide rail 10 is provided with a sliding block driving mechanism for driving the sliding block to move on the linear slide rail 10. Figure 2As shown, the slider driving mechanism comprises a driving motor 11 fixedly installed at one end of the linear slide rail 10, a lead screw 9 threaded through and installed on the slider, the front end of the lead screw 9 being connected to the end of the linear slide rail 10 through a bearing and the rear end being drivingly connected with the driving motor 11 through a shaft coupling, and the axis of the lead screw 9 being parallel to the sliding track of the linear slide rail 10. When the driving motor 11 works, the lead screw 9 is rotated, and when the lead screw 9 rotates, the slider sleeved on the lead screw 9 drives the servo motor 21 and the rotating shaft 2 to move along the axis of the lead screw 9. Of course, as other embodiments of the utility model, other structures of the slider driving mechanism can also be adopted, as long as the slider can be accurately driven to move on the linear slide rail 10.

[0038] After the test tube rack 8 is transferred to the test tube holder 22, the servo motor 21 works to drive the rotating shaft 2 and the test tube holder 22 to deflect by a certain angle, so that the test tube rack 8 is inclined to pour the reagent in the test tube on the micro-fluidic chip 7. The driving motor 11 can control the position of the rotating shaft 2 and the test tube holder 22, and the test tube holder 22 is moved above the micro-fluidic chip 7 before the reagent is added.

[0039] In order to realize continuous operation, a recycling mechanism 3 is arranged below the running track of the rotating shaft 2, and the recycling mechanism 3 is a belt conveyor with the conveying direction opposite to that of the horizontal conveying device 1. In this way, after the reagent is added, the test tube rack 8 together with the empty test tube can be discarded on the recycling mechanism 3 by turning over the test tube holder 22, and the recycling mechanism 3 can centrally process them.

[0040] The slide rail platform 5 comprises left and right slide grooves 501 and 502 arranged in parallel and capable of being simultaneously lifted, a heating platform 4 is arranged between the left and right slide grooves 501 and 502, and a second jacking device 504 is arranged outside the heating platform 4. The second jacking device 504 is two hydraulic cylinders symmetrically arranged on both sides of the heating platform 4, or can be screw rods.

[0041] In addition, the left and right slide grooves 501 and 502 are located inside the tracks of the two linear slide rails 10 and are parallel to the linear slide rails 10, and the lower ends of the left and right slide grooves 501 and 502 are provided with first jacking devices 503 for controlling the lifting of the left and right slide grooves 501 and 502. The first jacking devices 503 are four hydraulic cylinders respectively arranged at the front and rear ends of the left and right slide grooves 501 and 502, or can be screw rods.

[0042] The chip assembly holder 6 in the rectangular frame structure is slidably installed on the left and right slide grooves 501 and 502. A bearing platform 63 for clamping the micro-fluidic chip 7 is arranged inside the frame of the chip assembly holder 6, and the bearing platform 63 is a folded edge arranged inside the frame of the chip assembly holder 6.

[0043] AsFigure 1 , Figure 3 As shown, the four corners of the chip assembly bracket 6 are equipped with pulleys 62 via extension rods 61 perpendicular to the left slide groove 501 or the right slide groove 502. The pulleys 62 are installed in the left slide groove 501 or the right slide groove 502. When the first lifting device 503 is working, it drives the chip assembly bracket 6 to rise and fall in a horizontal state by raising and lowering the left slide groove 501 and the right slide groove 502. The second lifting device 504 is used to lift the extension rods 61. After the chip assembly bracket 6 is moved into place, the two extension rods 61 on the same side are exactly above the second lifting device 504. When the second lifting device 504 is working, it lifts one side of the chip assembly bracket 6, making the chip assembly bracket 6 higher on one side and lower on the other, in an inclined state. At this time, the microfluidic chip 7 on the chip assembly bracket 6 is also in an inclined state, which can promote the discharge of residual reagents in the microfluidic chip 7 and reduce reagent residue.

[0044] When in use, the test tube containing blood sample or enrichment reagent is installed on the test tube rack 8, and multiple test tubes can be installed on the same test tube rack 8, and the test tube rack 8 is placed on the horizontal conveying device 1; the rigid chip fixing piece 72 and the reagent groove 71 are placed on the chip assembly bracket 6, and the chip assembly bracket 6 without reagent is located between the test tube bracket 22 and the heating platform 4, and the chip body 721 is located in front of the heating platform 4. After starting work, the driving motor 11 first moves the rotating shaft 2 and the test tube bracket 22 to the initial position, above the recycling mechanism 3, between the horizontal conveying device 1 and the heating platform 4, close to the terminal end of the horizontal conveying device 1. Then, the horizontal conveying device 1 operates and transfers the test tube rack 8 to the test tube bracket 22; the driving motor 11 starts to move the rotating shaft 2 together with the test tube bracket 22 above the chip assembly bracket 6, and after the test tube bracket 22 is in place, the servo motor 21 operates to deflect the rotating shaft 2 and the test tube bracket 22 by a certain angle, and the blood sample in the test tube or the cell enrichment related reagent is poured into the reagent groove 71 to complete the reagent filling. After the reagent filling is completed, the servo motor 21 rotates in the opposite direction to discard the test tube and the test tube rack 8 onto the recycling mechanism 3, and the recycling mechanism 3 collects and processes the test tube rack 8 and the empty test tube. The servo motor 21 drives the test tube bracket 22 to return to the original position, and the sliding block driving mechanism drives the test tube bracket 22 to reset, preparing for the next reagent filling work cycle. After all the reagents are filled, the driving motor 11 starts to drive the test tube bracket 22 to move towards the heating platform 4, at this time the test tube bracket 22 is at the same height as the chip assembly bracket 6, and the test tube bracket 22 pushes the chip assembly bracket 6 to move above the heating platform 4. When the microfluidic chip 7 is pushed backward together with the chip assembly bracket 6 by the test tube bracket 22, the position of the reagent groove 71 and the chip body 721 is slightly higher than the upper edge of the heating platform 4, but the lower position of the suction assembly 74 is just blocked by the upper edge of the heating platform 4. When the reagent groove 71 moves backward together with the chip assembly bracket 6, the suction assembly 74 is blocked, the pawl 741 on the suction assembly 74 slides in the pawl groove 703 at the lower end of the reagent groove 71, until the pawl 741 is separated from the pawl groove 703, the suction assembly 74 falls off from the reagent groove 71 and falls onto the recycling mechanism 3 below, which is collected and disposed by the recycling mechanism 3. After the chip assembly bracket 6 is moved to the position, the height of the chip assembly bracket 6 can be lowered by the first lifting device 503 to place it on the heating platform 4, and then the above steps are repeated to pour the cell staining related reagent into the reagent groove 71 to complete the staining reagent filling. The heating platform 4 heats the chip body 721 to enhance the reactivity of the staining reagent, and after the staining is completed, the first lifting device 503 raises the height of the chip assembly bracket 6 to separate it from the heating platform 4, facilitating the staff to take the microfluidic chip 7.

[0045] As Figure 4As shown, the microfluidic chip 7 is used with the chip assembly holder 6, and multiple microfluidic chips 7 can be installed on the same chip assembly holder 6. The microfluidic chip 7 comprises a reagent groove 71, a liquid suction assembly 74 clamped below the reagent groove 71, a rigid chip fixing plate 72 installed between the reagent groove 71 and the liquid suction assembly 74, and a chip body 721 placed in the rigid chip fixing plate 72.

[0046] As shown in Figure 5 , Figure 6 , the reagent groove 71 is a sheet structure with a liquid leakage hole 702 longitudinally penetrating the body thereof, and a funnel-shaped liquid collection groove 701 is formed on the upper surface of the reagent groove 71, the lower end of the liquid collection groove 701 is in communication with the liquid leakage hole 702, and the upper end of the liquid collection groove 701 is rectangular and the lower end is circular. In use, the reagent groove 71 and the rigid chip fixing plate 72 are placed horizontally on the chip assembly holder 6, and blood samples or reagents and other liquids can be collected into the liquid leakage hole 702 along the inner wall of the liquid collection groove 701 and discharged after passing through the chip body 721.

[0047] As shown in Figure 7 , the material of the chip body 721 is preferably flexible glass, and the chip body 721 is uniformly distributed with multiple groups of long strip-shaped micropores, the size of the micropores allows reagents and other liquids to pass through but can intercept and capture circulating tumor cells with larger volumes, at least one diagonal line of the liquid collection groove 701 is parallel to the side edge of the reagent groove 71, and a drainage groove 705 is arranged on one diagonal of the liquid collection groove 701, the inner end of the drainage groove 705 is in communication with the liquid collection groove 701, and the outer end is in communication with the outside of the reagent groove 71. In this way, after cell staining is completed, the reagent groove 71 can be tilted by raising one side of the reagent groove 71, and the excess reagent in the reagent groove 71 can be concentrated and drained to the outside of the reagent groove 71 through the drainage groove 705, and then flow out along the chip assembly holder 6, which is conducive to the centralized disposal of waste reagents.

[0048] In addition, as shown in Figure 8As shown, the lower surface of the rigid chip fixing sheet 72 is provided with a liquid absorbing sheet 73, which includes a filter paper 731 pasted on the rigid chip fixing sheet 72, and a high polymer water absorbing material layer 732 provided between the filter paper 731 and the chip body 721. The high polymer water absorbing material layer 732 uses a powdered super absorbent resin, which is a new type of functional polymer material. It has a high water absorbing function of absorbing several hundred to several thousand times of water than its own weight, and has excellent water retention performance. Once it absorbs water and expands into a hydrogel, it is very difficult to separate the water even under pressure. Therefore, it has a wide range of applications in various fields such as personal hygiene products, industrial and agricultural production, civil construction, etc. The super absorbent resin generally contains a hydrophilic group and a cross-linked structure of a high polymer electrolyte. Before absorbing water, the high molecular chains are close to each other and entangled together, and are cross-linked into a network structure, so as to achieve overall tightening. When in contact with water, water molecules penetrate into the resin through capillary action and diffusion, and the ionized groups on the chain are ionized in water. Due to the electrostatic repulsion between the same ions on the chain, the high molecular chain is stretched and swells. Due to the requirement of electrical neutrality, counterions cannot migrate to the outside of the resin, and the difference in ion concentration between the inside and outside of the resin forms an osmotic pressure. Water further enters the resin under the action of the osmotic pressure, forming a hydrogel, which increases in volume and weight.

[0049] As shown in Figure 4 use, the edge of the filter paper 731 is pasted on the lower surface of the rigid chip fixing sheet 72 through water-soluble adhesive, so that the high polymer water absorbing material layer 732 is opposite to the chip body 721. After adding reagent, the high polymer water absorbing material layer 732 absorbs the reagent falling through the chip body 721, expands in volume and increases in weight, so that the liquid absorbing sheet 73 is separated from the rigid chip fixing sheet 72.

[0050] As shown in Figure 9As shown, the liquid suction assembly 74 is a container with an open upper end and a liquid leakage hole 702 on the reagent tank 71. The edge of the upper port of the liquid suction assembly 74 is provided with a clamping jaw 741, which is clamped in the clamping jaw groove 703 at the lower end of the reagent tank 71. After installation, the upper end of the liquid suction assembly 74 is opposite the chip body 721, and the waste reagent flowing out of the liquid leakage hole 702 and the detached liquid suction sheet 73 are temporarily stored in the liquid suction assembly 74. After the cell enrichment process is completed, due to the significant increase in volume and weight of the polymer water-absorbing material after absorbing water, the liquid suction assembly 74 is provided to prevent the polymer material from overflowing after absorbing water, reducing pollution to the surrounding environment. Because of the significant increase in volume and weight of the polymer water-absorbing material after absorbing water, the liquid suction sheet 73 automatically falls off the rigid chip fixing sheet 72 under the action of gravity and is stored in the liquid suction assembly 74, eliminating the need for manual peeling and collecting the liquid suction sheet 73, reducing manual labor and avoiding pollution. After the reagent is added, the microfluidic chip 7 is pushed backward together with the chip assembly bracket 6 by the test tube bracket 22, and the positions of the reagent tank 71 and the chip body 721 are slightly higher than the upper edge of the heating platform 4, but the lower liquid suction assembly 74 is blocked by the upper edge of the heating platform 4. When the reagent tank 71 moves backward with the chip assembly bracket 6, the liquid suction assembly 74 is blocked, the clamping jaw 741 on the liquid suction assembly 74 slides in the clamping jaw groove 703 at the lower end of the reagent tank 71, until the clamping jaw 741 is detached from the clamping jaw groove 703, the liquid suction assembly 74 is detached from the reagent tank 71, and falls onto the recovery mechanism 3 below, which is collected and disposed by the recovery mechanism 3.

[0051] In use, the rigid chip fixing sheet 72 is attached to the lower surface of the reagent tank 71, the liquid suction sheet 73 is located below the chip body 721, and the liquid suction assembly 74 is installed below the reagent tank 1. The blood sample or reagent is poured into the reagent tank 71, collected in the liquid collection tank 701, and then passes through the liquid leakage hole 702 and the chip body 721. The blood sample or reagent passing through the chip body 721 is absorbed by the liquid suction sheet 73, which contains a polymer water-absorbing material that expands by hundreds of times in volume after absorbing water. The liquid suction sheet 73 separates from the rigid chip fixing sheet 72 after absorbing the liquid. The liquid suction sheet 73 fully absorbing the reagent finally falls off the rigid chip fixing sheet 72 and falls into the liquid suction assembly 74, which is the cell enrichment process. Subsequently, at least one staining reagent is poured into the reagent tank 71, and after one staining reagent is stained, the second lifting device 504 lifts the extension rod 61 on one side of the reagent tank 71 to tilt the reagent tank 71. The reagent in the liquid collection tank 701 flows along the low drainage groove 705 to the lower part of the reagent tank 71, and finally flows out along the chip assembly bracket 6. Another staining reagent is poured into the reagent tank 1, and the above staining operation is repeated. The reagent in the liquid collection tank 106 is finally collected and disposed.

[0052] The CTC enrichment and dyeing integrated equipment is used for enriching and dyeing circulating tumor cells, and comprises the following steps:

[0053] Step 1) A plurality of test tubes containing blood samples or cell enrichment related reagents are fixed on a movable test tube rack 8, a plurality of microfluidic chips 7 are arranged on the movable chip assembly bracket 6 according to the spacing between the test tubes on the test tube rack 8, and a heating platform 4 is arranged below the chip assembly bracket 6;

[0054] Step 2) The test tube rack 8 is moved to the upper side of the chip assembly bracket 6, the test tube rack 8 is transferred to the turnover platform via the horizontal conveying device 1, the test tube rack 8 is moved to the upper side of the chip assembly bracket 6 by the turnover platform, and the test tube rack 8 is poured and the reagents in the plurality of test tubes are poured one by one and correspondingly poured onto the plurality of microfluidic chips 7. The reagents in each test tube on the test tube rack 8 are stored once, and the reagents in the test tube can be completely added to the corresponding microfluidic chip 7 when the test tube rack 8 is completely overturned. Since the spacing between the test tubes corresponds to the spacing between the microfluidic chips 7, the reagents in each test tube are added to the corresponding microfluidic chip 7 when the test tube rack 7 is completely overturned. And this embodiment uses a turnover platform as a carrier for pouring the test tube rack 8, which facilitates automatic mechanical operation and can accurately control the angle of the test tube rack 8 and the pouring of the reagents, thereby improving the operation efficiency and accurately controlling the amount of reagent addition.

[0055] Step 3) The chip assembly bracket 6 is horizontally moved by the horizontal conveying device 1, and the edge of the heating platform 4 blocks the liquid suction assembly 4 below the microfluidic chip 7, so that the liquid suction assembly 4 falls off the microfluidic chip 7;

[0056] Step 4) The microfluidic chip 7 separated from the liquid suction assembly 74 is moved to the heating platform 4, a plurality of test tubes containing cell dyeing related reagents are fixed on a movable test tube rack 8, step 2) is repeated, and the microfluidic chip is heated by the heating platform 4.

[0057] As a further improvement of the embodiment,

[0058] In step 2), a plurality of test tube racks 8 are arranged on the horizontal conveying device in turn, one test tube rack 8 is transferred to the turnover platform at a time, the test tube rack 8 is moved to the upper side of the chip assembly bracket 6 by the turnover platform, the test tube rack 8 is poured and the reagents in the plurality of test tubes are poured one by one and correspondingly poured onto the plurality of microfluidic chips 7, then the test tube rack 8 and the test tube on the turnover platform are removed, the next test tube rack 8 on the horizontal conveying device 1 is transferred to the turnover platform, the reagents are added to the microfluidic chip 7, and the reagents on all the test tube racks 8 are added until the reagents are added. Thus, the test tube rack 8 and the horizontal conveying device 1 cooperate with each other, a plurality of reagent additions can be realized by using the same device, and the utilization rate of the equipment is improved.

[0059] The content not described in the specification is the prior art known to those skilled in the art. Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art can modify the technical solutions recorded in the foregoing embodiments or make equivalent replacements to part of the technical features, and any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A CTC enrichment and staining integrated device, characterized by: The device comprises a horizontal conveying device (1) for horizontally moving a test tube rack (8), a turnover platform for overturning the test tube rack (8), a sliding rail platform (5) for mounting a microfluidic chip (7), the turnover platform is arranged at the terminal end of the horizontal conveying device (1), the chip assembly holder (6) of the rectangular frame structure for mounting the microfluidic chip (7) is slidingly arranged on the sliding rail platform (5), and the microfluidic chip (7) is provided with a chip body (721) for CTC enrichment and staining.

2. The integrated CTC enrichment and staining device of claim 1, wherein: The turnover platform comprises a rotating shaft (2) arranged at the terminal end of the horizontal conveying device (1), a servo motor (21) arranged at one end of the rotating shaft (2) and used for driving the rotating shaft (2) to rotate, and a test tube holder (22) fixedly connected to the rotating shaft (2) and used for receiving the test tube rack (8), the servo motor (21) is fixedly arranged on a sliding block, and the sliding block is slidingly arranged on a linear sliding rail (10).

3. The integrated CTC enrichment and staining device of claim 2, wherein: The sliding rail platform (5) comprises left and right sliding grooves (501 and 502) arranged in parallel to each other and capable of being lifted simultaneously, a heating platform (4) is arranged in the gap between the left and right sliding grooves (501 and 502), and the outer side of the heating platform (4) is provided with a second lifting device (504).

4. The integrated CTC enrichment and staining device of claim 3, wherein: The two linear sliding rails (10) are symmetrically arranged at the two ends of the rotating shaft (2), the two ends of the rotating shaft (2) are slidingly arranged on the linear sliding rails (10) through sliding blocks, the left and right sliding grooves (501 and 502) are located on the inner sides of the two linear sliding rails (10) and are parallel to the linear sliding rails (10), and the lower ends of the left and right sliding grooves (501 and 502) are provided with a first lifting device (503) used for controlling the left and right sliding grooves (501 and 502) to be lifted.

5. The integrated CTC enrichment and staining device of claim 1, wherein: The horizontal conveying device (1) is a belt conveyor.

6. The integrated CTC enrichment and staining device of claim 2, wherein: A recovery mechanism (3) is arranged below the running track of the rotating shaft (2), and the recovery mechanism (3) is a belt conveyor with a conveying direction opposite to that of the horizontal conveying device (1).

7. The integrated CTC enrichment and staining device of claim 3, wherein: The four corners of the chip assembly holder (6) are provided with pulleys (62) through extension rods (61) perpendicular to the left or right sliding groove (501 or 502), the pulleys (62) are arranged in the left or right sliding groove (501 or 502), and the second lifting device (504) is used for lifting the extension rod (61).

8. The integrated CTC enrichment and staining device of claim 1, wherein: The inside of the frame of the chip assembly holder (6) is provided with a supporting platform (63) used for clamping the microfluidic chip (7), and the supporting platform (63) is a folded edge arranged on the inner side of the frame of the chip assembly holder (6).

Images