Flow cytometer

Abstract

The utility model discloses a flow cytometry counter, relates to medical technical field, including device body, the inside wall of device body is fixedly connected with motor box, the inside of motor box is fixedly connected with motor body, the output end spline of motor body is connected with transmission rod, and the transmission rod is connected with the transmission rod. A transmission assembly is fixedly connected to one end of the transmission rod, a fixing ring is fixedly connected to the top of the transmission assembly, and a fixing assembly is fixedly connected to the inner side wall of the fixing ring; through the arrangement of the fixing ring and the fixing assembly, the fixing effect is achieved in the use process, the telescopic spring and the fixing soft plate in the fixing assembly can adapt to sample containers of different sizes, it is ensured that samples cannot shake or shift in the counting process through elastic clamping, cell analysis is more accurate, and the counting efficiency is improved. Errors caused by instability of the sample are reduced, the sample container can be prevented from being scratched or damaged by the fixing soft plate, and the integrity of the sample is protected.

Description

TECHNICAL FIELD

[0001] The utility model relates to the medical technology field, concretely is a flow cytometer. BACKGROUND

[0002] The flow cytometer is the advanced scientific and technological equipment of the integration of optics, electronics, fluid mechanics, cell chemistry, biology, immunology and laser and computer, is widely used in clinical medicine, cytology, biology, microbiology, pharmacy, reproductive science and other fields, is one of the advanced instruments in modern scientific research, it carries out qualitative, quantitative analysis or sorting technology to single cell or biological particle in fast straight flow state one by one, multiple parameters, fast, with fast detection speed, multiple measurement parameters, large data acquisition, comprehensive analysis, high purity of sorting, flexible method and other characteristics.

[0003] The utility model discloses a flow cytometer, including the cell instrument main part, the front surface of cell instrument main part and close to one side is equipped with the controller, the inside of cell instrument main part is opened with the detection groove, the inside upper portion of detection groove is movably installed with the detection head, the inside lower surface of detection groove is movably installed with the rotating frame, the inside close to both ends of rotating frame is movably embedded with the tray, the both sides of cell instrument main part and located the detection groove below are fixedly installed with the telescopic link, the telescopic end of telescopic link is fixedly installed with the motor, the output end of motor is fixedly installed with the transmission rod. The flow cytometer of the utility model, through the mode of mutual cooperation of cell instrument main part, rotating frame, tray, transmission rod, motor and telescopic link, the rotating action of tray and rotating frame, only need to pass through a motor and can carry out control.

[0004] The above technical scheme is used,

[0005] (1) in the sample fixation aspect, the existing fixed mode is often not flexible enough, it is difficult to adapt to different size and shape sample container, and it is easy to cause sample to shake or shift in the analysis process, influence the accuracy of analysis result;

[0006] (2) for the adjustment of sample position, the traditional flow cytometer usually adopts manual adjustment mode, not only operation is tedious, and it is difficult to realize accurate position control.

[0007] In view of the above problems, the utility model provides a flow cytometer. Utility model content

[0008] The utility model discloses a purpose at providing a kind of flow cytometer, the utility model can be fixed to the sample tube of different diameter size, and the height of sample tube can be accurately controlled, to solve the problem in background art.

[0009] To achieve the above object, the utility model provides the following technical scheme: a kind of flow cytometer, including device ontology, the inner side wall of the device ontology is fixedly connected with motor box, the inside of the motor box is fixedly connected with motor ontology, the output end spline of the motor ontology is connected with transmission rod, one end of the transmission rod is fixedly connected with transmission assembly, the top of the transmission assembly is fixedly connected with fixed ring, the inner side wall of the fixed ring is fixedly connected with fixed assembly, the inner top of the device ontology is fixedly connected with pump body, one side of the pump body is fixedly connected with suction needle, the other side of the pump body is fixedly connected with connecting pipe, one side of the connecting pipe is inserted with flow chamber.

[0010] Further, the transmission assembly includes a threaded rod fixedly connected to one end of the transmission rod, and a support plate is threadedly connected to one side of the threaded rod, achieving the effect of supporting the sample test tube.

[0011] Further, the fixed assembly includes a telescopic spring fixedly connected to the inner side wall of the fixed ring, and a fixed soft plate is fixedly connected to one side of the telescopic spring, achieving the effect of protection.

[0012] Further, one side of the flow chamber is fixedly connected with a sheath liquid tube, and one side of the sheath liquid tube is fixedly connected with a plug opening, achieving the effect of plugging.

[0013] Further, one side of the device ontology is fixedly connected with a laser, and the other side of the device ontology is fixedly connected with a positive-negative charge deflection plate, achieving the effect of separation and classification.

[0014] Further, the bottom of the device ontology is slidingly connected with a three-way waste liquid box, and one side of the three-way waste liquid box is fixedly connected with a handle, achieving the effect of sliding.

[0015] Compared with the prior art, the utility model has the following beneficial effects:

[0016] The utility model provides a kind of flow cytometer,

[0017] (1) through the setting of fixed ring and fixed assembly, the effect of fixation is achieved when using, the telescopic spring and fixed soft plate in fixed assembly can adapt to different sizes of sample container, elastic clamping is ensured that sample does not shake or shift during counting process, which makes cell analysis more accurate, reduces the error caused by unstable sample, fixed soft plate can avoid scratching or damaging sample container, protects the integrity of sample.

[0018] (2) Through the setting of the transmission assembly and the pump body, the adjusting effect is achieved in use, the cooperation of the motor body, the transmission rod and the threaded rod in the transmission assembly can realize the accurate adjustment of the sample height, which makes the suction needle can accurately suck the sample, ensures that the position and angle of the sample entering the flow chamber are optimal, and improves the accuracy and repeatability of cell counting. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is a device body schematic view of the utility model;

[0020] Figure 2 It is a telescopic spring schematic view of the utility model;

[0021] Figure 3 It is a flow chamber schematic view of the utility model;

[0022] Figure 4 It is a suction needle schematic view of the utility model.

[0023] In the drawing: 1, device body;2, motor box;3, motor body;4, transmission rod;5, transmission assembly;501, threaded rod;502, support plate;6, fixed ring;7, fixed assembly;701, telescopic spring;702, fixed soft plate;8, pump body;9, suction needle;10, connecting pipe;11, flow chamber;12, sheath liquid pipe;13, blockage port;14, laser;15, positive and negative charge deflection plate;16, three-way waste liquid box;17, handle. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0025] In order to solve how to effectively position the adjusting technical problem, such as Figures 1-4 As shown in the figure, the following preferred technical solutions are provided:

[0026] A flow cytometer, comprising a device body 1, the inner side wall of the device body 1 is fixedly connected with a motor box 2, through the setting of the motor box 2, the motor body 3 can be protected inside the motor box 2, avoiding the motor body 3 exposed to damage, thereby achieving the effect of protection, the inside of the motor box 2 is fixedly connected with the motor body 3, the output end of the motor body 3 is spline connected with a transmission rod 4, through the setting of the transmission rod 4, it can rotate under the driving of the motor body 3, thereby achieving the effect of rotation, one end of the transmission rod 4 is fixedly connected with a transmission assembly 5, the top of the transmission assembly 5 is fixedly connected with a fixed ring 6, the inner side wall of the fixed ring 6 is fixedly connected with a fixing assembly 7, through the setting of the fixed ring 6 and the fixing assembly 7, the effect of fixation is achieved during use, the extension spring 701 and the fixed soft plate 702 in the fixing assembly 7 can adapt to different sizes of sample containers, through elastic clamping, it ensures that the sample will not shake or shift during counting, which makes the cell analysis more accurate, reduces the error caused by unstable sample, the fixed soft plate 702 can avoid scratching or damaging the sample container, protecting the integrity of the sample, thereby achieving the effect of fixation, the inner top of the device body 1 is fixedly connected with a pump body 8, one side of the pump body 8 is fixedly connected with a suction needle 9, through the setting of the transmission assembly 5 and the pump body 8, the effect of adjustment is achieved during use, the cooperation of the motor body 3, the transmission rod 4 and the threaded rod 501 in the transmission assembly 5 can realize accurate adjustment of the sample height, which makes the suction needle 9 can accurately suck the sample, ensuring that the sample enters the flow chamber 11 at the best position and angle, improving the accuracy and repeatability of cell counting, thereby achieving the effect of accuracy, the other side of the pump body 8 is fixedly connected with a connecting pipe 10, one side of the connecting pipe 10 is inserted with a flow chamber 11.

[0027] Specifically, turn on the power switch of the flow cytometer, start the motor body 3, observe the relative position of the sample container and the suction needle 9 through the observation window or display screen on the equipment, according to the observed position, control the forward and reverse rotation of the motor body 3, drive the transmission rod 4 and the threaded rod 501 to rotate, make the support plate 502 rise or fall, thereby adjust the height of the sample container, make the opening of the sample container align with the suction needle 9.

[0028] Further, as shown in Figure 2 , the following preferred technical solutions are provided:

[0029] The transmission assembly 5 comprises a threaded rod 501 fixedly connected to one end of the transmission rod 4, one side of the threaded rod 501 is threadedly connected with a support plate 502, the purpose of this design is that the support plate 502 can move under the rotation of the threaded rod 501, thereby achieving the effect of movement.

[0030] Further, as shown in Figure 2 , the following preferred technical solutions are provided:

[0031] The fixed assembly 7 comprises a telescopic spring 701 fixedly connected to the inner side wall of the fixed ring 6, and one side of the telescopic spring 701 is fixedly connected with a fixed soft plate 702, which is designed to fix the sample tube through the fixed soft plate 702, so as to achieve the fixed effect.

[0032] Further, as shown in Figure 3 The preferred technical solutions are provided as follows:

[0033] One side of the flow chamber 11 is fixedly connected with a sheath liquid pipe 12, and one side of the sheath liquid pipe 12 is fixedly connected with a plugging port 13, which is designed to plug the sheath liquid pipe 12 through the plugging port 13, so as to achieve the effect of sealing and protection.

[0034] Further, as shown in Figure 3 The preferred technical solutions are provided as follows:

[0035] One side of the device body 1 is fixedly connected with a laser 14, and the other side of the device body 1 is fixedly connected with a positive and negative charge deflection plate 15, which is designed to separate cells, so as to achieve the effect of separation.

[0036] Further, as shown in Figure 1 The preferred technical solutions are provided as follows:

[0037] The bottom of the device body 1 is slidingly connected with a three-way waste liquid box 16, and one side of the three-way waste liquid box 16 is fixedly connected with a handle 17, which is designed to facilitate pulling out the three-way waste liquid box 16, so as to achieve the effect of extraction.

[0038] In summary:

[0039] 1. Gently pull the fixed soft plate 702 on the fixed ring 6 to expand the opening of the fixed ring 6, place the sample container, carefully place the container containing the cell sample into the fixed ring 6, ensure that the bottom of the container is in good contact with the fixed ring 6, loosen the fixed soft plate 702, let the elastic force of the telescopic spring 701 act on the fixed soft plate 702, so that the fixed soft plate 702 tightly clamps the sample container, and ensure that the sample container is stable in the fixed ring 6 without shaking;

[0040] 2. Turn on the power switch of the flow cytometer, start the motor body 3, observe the relative position of the sample container and the suction needle 9 through the observation window or display screen on the equipment, and according to the observed position, control the forward and reverse rotation of the motor body 3 to drive the transmission rod 4 and the threaded rod 501 to rotate, so that the support plate 502 rises or falls, thereby adjusting the height of the sample container, and aligning the opening of the sample container with the suction needle 9;

[0041] 3, ensure that the sheath liquid pipe 12 and flow chamber 11 and sheath liquid source connection firm, no leakage, slowly open the valve of sheath liquid source, let the sheath liquid into the sheath liquid pipe 12, observe the flow of liquid in the flow chamber 11, by adjusting the valve of sheath liquid source or sheath liquid flow control device on the equipment, adjust the flow and pressure of sheath liquid, make the sample and sheath liquid fully mixed, form stable liquid flow, open the switch of pump body 8, let the pump body 8 start working, through the needle 9 to take the sample, and send it into the flow chamber 11, open the power switch of laser 14, let the laser 14 emit laser, excite cell to produce fluorescence signal, through the display screen or data analysis software on the equipment, observe the counting condition and fluorescence signal intensity of cell, record the relevant data.

[0042] It should be noted that, in this text, such as the first and second relationship terms are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between the entities or operations. Moreover, the term "includes", "contains" or any other variant thereof is intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements not only includes those elements, but also includes other elements not explicitly listed, or includes elements inherent in such process, method, article or equipment.

[0043] Although the embodiments of the present application have been shown and described, it is understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present application, the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A flow cytometer comprising a device body (1), characterised in that: The inner side wall of the device body (1) is fixedly connected with a motor box (2), the inside of the motor box (2) is fixedly connected with a motor body (3), the output end of the motor body (3) is spline connected with a transmission rod (4), one end of the transmission rod (4) is fixedly connected with a transmission assembly (5), the top of the transmission assembly (5) is fixedly connected with a fixed ring (6), the inner side wall of the fixed ring (6) is fixedly connected with a fixed assembly (7), the inner top of the device body (1) is fixedly connected with a pump body (8), one side of the pump body (8) is fixedly connected with a suction needle (9), the other side of the pump body (8) is fixedly connected with a connecting pipe (10), one side of the connecting pipe (10) is inserted with a flow chamber (11).

2. A flow cytometer according to claim 1, wherein: The transmission assembly (5) comprises a threaded rod (501) fixedly connected to one end of the transmission rod (4), and the threaded rod (501) is screw connected with a support plate (502) on one side.

3. A flow cytometer according to claim 1, wherein: The fixed assembly (7) comprises a telescopic spring (701) fixedly connected to the inner side wall of the fixed ring (6), and the telescopic spring (701) is fixedly connected with a fixed soft plate (702) on one side.

4. A flow cytometer according to claim 1, wherein: One side of the flow chamber (11) is fixedly connected with a sheath liquid pipe (12), and the sheath liquid pipe (12) is fixedly connected with a blockage port (13) on one side.

5. A flow cytometer according to claim 1, wherein: One side of the device body (1) is fixedly connected with a laser (14), and the other side of the device body (1) is fixedly connected with a positive and negative charge deflection plate (15).

6. A flow cytometer according to claim 1, wherein: The bottom of the device body (1) is slidably connected with a three-way waste liquid box (16), and one side of the three-way waste liquid box (16) is fixedly connected with a handle (17).

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