Target protein separation and purification device

Abstract

The utility model relates to the technical field of protein separation and purification, in particular to a target protein separation and purification device which comprises a vertical plate, a pressure control assembly arranged on the left side of the vertical plate, a separation and purification tank arranged on the right side of the vertical plate, a heat preservation assembly arranged on the outer side of the separation and purification tank and a detachable inner cylinder arranged in the separation and purification tank. A filter membrane for filtering is horizontally and fixedly arranged in the inner cylinder; the pressure control assembly comprises an inflator, the inflator is arranged on the left side of the vertical plate and fixedly connected with the vertical plate through two first tension bands, an electric push rod is installed at the upper end of the inflator, a piston plate matched with the inflator is assembled in the inflator, an air pipe is arranged on the right side of the bottom of the inflator, and the right end of the air pipe is fixedly connected to a sealing cover. The pressure in the separation and purification tank can be regulated and controlled through the arranged pressure control assembly, the chromatographic purification process of protein on a filter membrane is accelerated, the protein purification speed is remarkably increased, the protein purification process is more efficient due to the controllability of the pressure, and rapid purification of target protein is facilitated.

Description

Technical Field

[0001] This utility model relates to the field of protein separation and purification technology, and more specifically, to a target protein separation and purification device. Background Technology

[0002] Protein separation and purification is the process of extracting target proteins from biological samples and removing impurities, aiming to obtain high-purity protein preparations for subsequent functional studies, structural analysis, or biopharmaceutical preparation. Proteins are among the most important biological macromolecules in living organisms, participating in all aspects of life activities. To study the essence of life, it is necessary to purify proteins from biological materials and study their structure and function, which is of great significance for understanding the laws governing life activities and elucidating the essence of life phenomena. Simultaneously, in industrial production, such as in the food, fermentation, textile, and leather industries, large quantities of highly active enzyme preparations are needed, and the preparation of these enzyme preparations also requires protein separation and purification techniques.

[0003] Protein purification is a crucial step in life science research, biopharmaceutical development, and industrial production. Currently, commonly used protein purification methods include molecular sieve chromatography, affinity chromatography, and ion exchange chromatography. However, protein purification typically relies on gravity to allow proteins to settle naturally, resulting in slow purification speeds and low efficiency, which is not conducive to the rapid purification of target proteins.

[0004] In view of this, the present invention proposes a separation and purification device that can accelerate the precipitation and separation of target proteins and improve efficiency. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides a target protein separation and purification device, which solves the problems mentioned in the background section.

[0006] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0007] A target protein separation and purification device includes a base and a vertical plate. The vertical plate is fixedly mounted on the upper surface of the base. A pressure control component is provided on the left side of the vertical plate. A separation and purification tank is provided on the right side of the vertical plate. A heat preservation component is provided on the outside of the separation and purification tank. A detachable inner cylinder is provided inside the separation and purification tank. A filter membrane for filtration is horizontally fixed inside the inner cylinder. The bottom of the inner cylinder is designed as a cone shape and is provided with a dropper. A sealing cap is installed at the top of the separation and purification tank by screws.

[0008] The pressure control assembly includes an electric push rod, an air cylinder, a pressure control valve, an air pipe, and a piston plate. The air cylinder is located on the left side of the upright plate and is fixedly connected to the upright plate by two sets of first hoops. An electric push rod is installed at the upper end of the air cylinder, and a matching piston plate is assembled inside the air cylinder. The output end of the electric push rod extends into the air cylinder and is fixedly connected to the piston plate. An air pipe is provided on the bottom right side of the air cylinder, and the right end of the air pipe is fixedly connected to a sealing cap. A pressure control valve is provided on the air pipe.

[0009] Furthermore, a pressure relief valve is installed on the left side of the bottom of the air cylinder.

[0010] Furthermore, the middle section of the trachea is provided with a filter cylinder, and the filter cylinder is filled with filter media.

[0011] Furthermore, the sealing cap is connected to an infusion tube for delivering the protein solution, and the infusion tube is equipped with an infusion valve. The bottom of the separation and purification tank has a conical structure and is provided with an outlet pipe, and the outlet pipe is equipped with an outlet valve.

[0012] Furthermore, a pressure gauge is installed on the sealing cover.

[0013] Furthermore, the insulation component includes an insulation sleeve, an outlet pipe, a control valve, and an inlet pipe. The insulation sleeve is a hollow structure and is fixedly fitted onto the outside of the separation and purification tank. An inlet pipe for conveying external cold air is fixedly connected to the bottom of the insulation sleeve ring, and an outlet pipe for discharging cold air is fixedly connected to the top of the insulation sleeve ring. Both the outlet pipe and the inlet pipe are equipped with control valves.

[0014] Furthermore, the outer side of the thermal insulation sleeve is fixedly fitted with two sets of second hoops, which are fixedly connected to the upright plate.

[0015] Furthermore, a retaining ring is fixed on the inner wall of the separation and purification tank near the top. The inner diameter of the retaining ring is larger than that of the inner cylinder, and the upper end of the inner cylinder rests on the retaining ring.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] 1. This utility model can regulate the pressure inside the separation and purification tank through the set pressure control component, which can accelerate the chromatographic purification process of protein on the filter membrane, significantly improve the speed of protein purification, and make the protein purification process more efficient through pressure controllability, which is conducive to the rapid purification of target protein.

[0018] 2. This utility model can cool and keep the inside of the separation and purification tank by setting the heat preservation component. The heat preservation sleeve can reduce the impact of external temperature fluctuations on the temperature inside the separation and purification tank and keep the temperature inside the tank relatively stable. The air inlet pipe is used to deliver external cold air into the cavity of the heat preservation sleeve to reduce the temperature inside the tank and ensure the activity of the purified protein. The air outlet pipe is used to discharge the cold air in the cavity of the heat preservation sleeve to realize the circulation of cold air inside the heat preservation sleeve.

[0019] 3. In this invention, a filter cylinder is provided in the middle section of the gas tube, and the filter cylinder is filled with filter media. The filter cylinder and the filter media inside can effectively remove impurities, particles and possible contaminants from the gas. By filtering the gas, impurities can be prevented from entering the separation and purification tank, thereby preventing impurities from affecting the purification effect and activity of the protein.

[0020] 4. A pressure gauge is installed on the sealing cover of this utility model. The pressure gauge can display the pressure value inside the separation and purification tank in real time, helping the operator to understand the pressure status inside the tank. By monitoring the pressure, the operator can adjust the working status of the pressure control component in a timely manner to avoid damage to the device or affect the protein separation and purification effect due to excessive pressure inside the tank, so as to ensure the efficiency of protein separation and purification.

[0021] 5. The separation and purification tank of this utility model is provided with a detachable inner cylinder. A filter membrane for filtration is horizontally fixed inside the inner cylinder, which is convenient for cleaning and replacement, and allows for the replacement of filter membranes of different specifications according to different protein purification needs. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0023] Figure 2 This is a schematic diagram of the structure of this utility model from another angle.

[0024] Figure 3 This is a schematic diagram of the pressure control component in this utility model.

[0025] Figure 4 This is a cross-sectional view of the air cylinder in this utility model.

[0026] Figure 5 This is a schematic diagram of the separation and purification tank in this utility model.

[0027] Figure 6 This is a cross-sectional view of the separation and purification tank in this utility model.

[0028] In the diagram: 1. Base; 2. Pressure control assembly; 21. Electric push rod; 22. Air cylinder; 23. First hoop; 24. Pressure control valve; 25. Filter cartridge; 26. Air pipe; 27. Piston plate; 28. Pressure relief valve; 3. Vertical plate; 4. Insulation assembly; 41. Insulation sleeve; 42. Air outlet pipe; 43. Control valve; 44. Air inlet pipe; 5. Sealing cap; 6. Pressure gauge; 7. Separation and purification tank; 71. Retaining ring; 8. Second hoop; 9. Infusion pipe; 10. Infusion valve; 11. Discharge valve; 12. Discharge pipe; 13. Inner cylinder; 14. Filter membrane; 15. Dropper. Detailed Implementation

[0029] The technical solutions of this utility model will be clearly and completely described below with reference to the embodiments of this utility model. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of this utility model.

[0030] Example:

[0031] like Figures 1 to 6 As shown, a target protein separation and purification device includes a base 1 and a vertical plate 3. The vertical plate 3 is fixedly mounted on the upper surface of the base 1. A pressure control component 2 is provided on the left side of the vertical plate 3, and a separation and purification tank 7 is provided on the right side of the vertical plate 3. A heat preservation component 4 is provided on the outside of the separation and purification tank 7. A detachable inner cylinder 13 is provided inside the separation and purification tank 7 for easy cleaning and replacement. It is also convenient to replace the filter membrane 14 of different specifications according to different protein purification needs. A filter membrane 14 for filtration is horizontally fixed inside the inner cylinder 13. The bottom of the inner cylinder 13 is set with a conical structure and is provided with a dropper 15. A sealing cap 5 is installed at the upper end of the separation and purification tank 7 by screws.

[0032] The pressure control assembly 2 includes an electric push rod 21, an air cylinder 22, a pressure control valve 24, an air pipe 26, and a piston plate 27. The air cylinder 22 is located on the left side of the vertical plate 3 and is fixedly connected to the vertical plate 3 through two sets of first hoops 23. An electric push rod 21 is installed at the upper end of the air cylinder 22. A matching piston plate 27 is installed inside the air cylinder 22. The output end of the electric push rod 21 extends into the air cylinder 22 and is fixedly connected to the piston plate 27. An air pipe 26 is located on the bottom right side of the air cylinder 22. The right end of the air pipe 26 is fixedly connected to the sealing cap 5. A pressure control valve 24 is installed on the air pipe 26. This design solves the problem that existing protein separation and purification devices usually rely on gravity to allow proteins to sink naturally during protein chromatography purification, resulting in slow protein purification speed and low efficiency, which is not conducive to the rapid purification of target proteins.

[0033] This invention accelerates the chromatographic purification process of proteins on the filter membrane 14 by using the pressure generated by the pressure control component 2, significantly improving the speed of protein purification. The controllability of the pressure makes the protein purification process more efficient, which is conducive to the rapid purification of the target protein. At the same time, the electric push rod 21 and the pressure control valve 24 make the operation more convenient and reduce the complexity of manual operation.

[0034] A pressure relief valve 28 is installed on the bottom left side of the air cylinder 22. The pressure relief valve 28 can be used to regulate the pressure inside the air cylinder 22. The pressure relief valve 28 can be used in conjunction with the pressure control valve 24 to achieve more precise pressure control. When it is necessary to reduce the pressure, the pressure relief valve 28 can be manually opened to gradually reduce the pressure inside the air cylinder 22 to the required level.

[0035] A filter cartridge 25 is installed in the middle section of the gas tube 26, and the filter cartridge 25 is filled with filter media. The filter cartridge 25 and the filter media inside it can effectively remove impurities, particles and possible contaminants from the gas. By filtering the gas, impurities can be prevented from entering the separation and purification tank 7, thereby preventing impurities from affecting the purification effect and activity of the protein.

[0036] A delivery tube 9 for conveying the protein solution is connected to the sealing cap 5. A delivery valve 10 is installed on the delivery tube 9. The bottom of the separation and purification tank 7 has a conical structure and an outlet tube 12, on which an outlet valve 11 is installed. The delivery tube 9 is used to deliver the protein solution from an external source into the separation and purification tank 7 and into the inner cylinder 13. The delivery valve 10, installed on the delivery tube 9, controls the flow rate of the protein solution. By opening or closing the valve, the inflow rate of the protein solution can be precisely adjusted. The outlet tube 12 is used to discharge the purified protein solution, ensuring that the purified protein solution flows smoothly out of the tank for further processing or analysis. The outlet valve 11, installed on the outlet tube 12, controls the opening and closing of the outlet tube 12.

[0037] A pressure gauge 6 is installed on the sealing cover 5. The pressure gauge 6 can display the pressure value inside the separation and purification tank 7 in real time, helping the operator to understand the pressure status inside the tank. By monitoring the pressure, the operator can adjust the working status of the pressure control component 2 in a timely manner to avoid damage to the device or affect the protein separation and purification effect due to excessive pressure inside the tank, so as to ensure the efficiency of protein separation and purification.

[0038] The insulation component 4 includes an insulation sleeve 41, an outlet pipe 42, a control valve 43, and an inlet pipe 44. The insulation sleeve 41 is a hollow structure and is fixedly fitted onto the outside of the separation and purification tank 7. An inlet pipe 44 for supplying external cold air is fixedly connected to the bottom of the ring surface of the insulation sleeve 41, and an outlet pipe 42 for discharging cold air is fixedly connected to the top of the ring surface of the insulation sleeve 41. Both the outlet pipe 42 and the inlet pipe 44 are equipped with control valves 43. The insulation sleeve 41 can reduce the impact of external temperature fluctuations on the temperature inside the separation and purification tank 7, maintaining a relatively stable temperature inside the tank. The inlet pipe 44 is used to supply external cold air into the cavity of the insulation sleeve 41 to lower the temperature inside the tank and ensure the activity of the purified protein. The outlet pipe 42 is used to discharge the cold air inside the cavity of the insulation sleeve 41, realizing the circulation of cold air inside the insulation sleeve 41.

[0039] Two sets of second hoops 8 are fixedly fitted on the outer side of the insulation sleeve 41. The second hoops 8 are fixedly connected to the vertical plate 3. The second hoops 8 provide additional support and fixation for the insulation sleeve 41, thereby achieving support and fixation for the separation and purification tank 7.

[0040] A retaining ring 71 is fixed on the inner wall of the separation and purification tank 7 near the top. The inner diameter of the retaining ring 71 is larger than that of the inner cylinder 13. The upper end of the inner cylinder 13 rests on the retaining ring 71. The main function of the retaining ring 71 is to support the inner cylinder 13, ensure that the inner cylinder 13 maintains a stable position in the separation and purification tank 7, and facilitate the installation and disassembly of the inner cylinder 13.

[0041] The working principle of this target protein separation and purification device:

[0042] In practical use, a suitable filter membrane 14 is selected and installed in the inner cylinder 13 according to the protein purification requirements, and the inner cylinder 13 is placed on the retaining ring 71 inside the separation and purification tank 7. The infusion valve 10 is opened, and the protein solution is delivered from an external source to the separation and purification tank 7 through the infusion tube 9, flowing into the inner cylinder 13. The infusion valve 10 can control the flow rate of the protein solution, adjusting the inflow rate as needed. The protein solution is purified by filtration through the filter membrane 14 in the inner cylinder 13, and the purified protein is discharged through the dropper 15 at the bottom of the inner cylinder 13, accumulating at the bottom of the separation and purification tank 7. During purification, the electric push rod 21 is activated, pushing the piston plate 27 downwards within the gas cylinder 22, thereby generating pressure and increasing the gas pressure in the separation and purification tank 7, promoting protein filtration on the filter membrane 14 and accelerating the chromatographic purification process. The pressure gauge 6 displays the pressure value inside the tank in real time, allowing the operator to adjust the working status of the pressure control component 2 according to the pressure conditions. To reduce pressure, the pressure relief valve 28 can be manually opened to gradually lower the pressure inside the gas cylinder 22 to the desired level. The purified protein solution is discharged from the tank through the outlet pipe 12 for further processing or analysis.

[0043] Simultaneously, external cold air is supplied to the cavity of the insulation sleeve 41 through the air inlet pipe 44 to reduce the temperature inside the tank and ensure the activity of the purified protein. The air outlet pipe 42 is used to discharge the cold air inside the cavity of the insulation sleeve 41, realizing cold air circulation. The filter cartridge 25 in the middle section of the air pipe 26 and its internal filter packing effectively remove impurities in the gas, preventing impurities from entering the separation and purification tank 7 and affecting the purification effect and activity of the protein. After the protein purification is completed, the inner cylinder 13 is disassembled for cleaning for the next use. At the same time, different specifications of filter membranes 14 can be replaced according to different protein purification needs.

[0044] In summary, this target protein separation and purification device accelerates the chromatographic purification process of the protein on the filter membrane 14 by using the pressure generated by the pressure control component 2, maintains a relatively stable temperature inside the tank by the heat preservation component 4, and removes impurities from the gas by using the filter cartridge 25. This device is easy to operate, has high purification efficiency, and is beneficial for the rapid purification of target proteins.

[0045] Obviously, the above embodiments of this utility model are merely examples for clearly illustrating this utility model, and are not intended to limit the implementation of this utility model. For those skilled in the art, other variations or modifications can be made based on the above description. It is impossible to exhaustively list all the implementation methods here. Any obvious variations or modifications derived from the technical solutions of this utility model are still within the protection scope of this utility model.

Claims

1. A target protein separation and purification device, comprising a base (1) and a vertical plate (3), characterized in that: A vertical plate (3) is fixedly provided on the upper surface of the base (1). A pressure control component (2) is provided on the left side of the vertical plate (3). A separation and purification tank (7) is provided on the right side of the vertical plate (3). A heat preservation component (4) is provided on the outside of the separation and purification tank (7). A detachable inner cylinder (13) is provided inside the separation and purification tank (7). A filter membrane (14) for filtration is horizontally fixed inside the inner cylinder (13). The bottom of the inner cylinder (13) is a conical structure and is provided with a dropper (15). A sealing cap (5) is installed on the upper end of the separation and purification tank (7) by screws. The pressure control assembly (2) includes an electric push rod (21), an air cylinder (22), a pressure control valve (24), an air pipe (26), and a piston plate (27). The air cylinder (22) is located on the left side of the upright plate (3) and is fixedly connected to the upright plate (3) through two sets of first hoops (23). An electric push rod (21) is installed at the upper end of the air cylinder (22). A piston plate (27) is fitted inside the air cylinder (22). The output end of the electric push rod (21) extends into the air cylinder (22) and is fixedly connected to the piston plate (27). An air pipe (26) is provided on the right side of the bottom of the air cylinder (22). The right end of the air pipe (26) is fixedly connected to the sealing cover (5). A pressure control valve (24) is provided on the air pipe (26).

2. The target protein separation and purification apparatus according to claim 1, characterized in that: A pressure relief valve (28) is installed on the left side of the bottom of the air cylinder (22).

3. The target protein separation and purification apparatus according to claim 2, characterized in that: The middle section of the trachea (26) is provided with a filter cylinder (25), and the filter cylinder (25) is filled with filter material.

4. The target protein separation and purification apparatus according to claim 1, characterized in that: The sealing cap (5) is connected to an infusion tube (9) for conveying protein solution. An infusion valve (10) is installed on the infusion tube (9). The bottom of the separation and purification tank (7) is conical and is provided with an outlet pipe (12). An outlet valve (11) is installed on the outlet pipe (12).

5. The target protein separation and purification apparatus according to claim 4, characterized in that: A pressure gauge (6) is installed on the sealing cover (5).

6. The target protein separation and purification apparatus according to claim 1, characterized in that: The insulation component (4) includes an insulation sleeve (41), an outlet pipe (42), a control valve (43), and an inlet pipe (44). The insulation sleeve (41) is a cavity structure and is fixedly fitted on the outside of the separation and purification tank (7). An inlet pipe (44) for conveying external cold air is fixedly connected to the bottom of the ring surface of the insulation sleeve (41). An outlet pipe (42) for discharging cold air is fixedly connected to the top of the ring surface of the insulation sleeve (41). A control valve (43) is installed on both the outlet pipe (42) and the inlet pipe (44).

7. The target protein separation and purification apparatus according to claim 6, characterized in that: The outer side of the thermal insulation sleeve (41) is fixedly fitted with two sets of second hoops (8), which are fixedly connected to the upright plate (3).

8. The target protein separation and purification apparatus according to claim 1, characterized in that: A retaining ring (71) is fixed on the inner wall of the separation and purification tank (7) near the top. The inner diameter of the retaining ring (71) is larger than that of the inner cylinder (13), and the upper end of the inner cylinder (13) rests on the retaining ring (71).

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