Cell culture fluid separation and purification device

Abstract

The utility model discloses a cell culture fluid separation and purification device, which relates to the technical field of cell culture and is characterized in that a turnover brake mechanism for driving a turnover shaft is arranged on one side of a support of a standing frame, a turnover frame is arranged in the middle of a shaft rod of the turnover shaft, and a centrifugal purification component is mounted at the front end of a support of the turnover frame; a centrifugal net cylinder is rotationally connected into a tank body of the centrifugal tank, and a centrifugal brake mechanism for driving the centrifugal net cylinder is arranged at the upper end of the tank body of the centrifugal tank. According to the cell culture fluid separation and purification device, the overturning brake mechanism is used as an overturning brake source to drive the centrifugal tank to overturn and rotate, the centrifugal tank is flexibly switched among a vertical state, an inclined state and a horizontal state, and during switching, the centrifugal brake mechanism drives the centrifugal mesh cylinder to centrifugally rotate, so that the cell culture fluid is separated and purified. The cell culture fluid is separated and purified in different states, the separation and purification are more diversified, the centrifugal separation state is more changeable, and the separation efficiency is higher.

Description

Technical Field

[0001] This utility model relates to the field of cell culture, and in particular to a cell culture medium separation and purification device. Background Technology

[0002] Cell growth requires a certain nutritional environment. The nutrient substrate used to maintain cell growth is called culture medium. Cell culture medium is the material basis for providing cell nutrition and promoting cell growth and proliferation. In order to ensure the accuracy and reliability of experimental results, separation and purification equipment is usually used to separate and purify cell culture medium. As shown in the stem cell culture medium separation and purification equipment disclosed on the China Patent Network (publication announcement number CN216946978U), this type of cell culture medium separation and purification equipment uses a fixed mechanism to seal the culture chamber, and then uses a rotary motor to drive the rotating rod to rotate. The rotation of the rotating rod drives the chamber to rotate, which facilitates the centrifugation separation of stem cells in the chamber.

[0003] However, the cell culture medium separation and purification equipment disclosed in the aforementioned patents and currently available in the market still has some shortcomings: the existing method of using braking components to propel the cell culture medium for horizontal centrifugation purification has a relatively simple centrifugation state, resulting in low separation and purification efficiency and long processing time. Therefore, those skilled in the art have provided a cell culture medium separation and purification device to solve the problems mentioned in the background section. Utility Model Content

[0004] To address the shortcomings of existing technologies, this invention provides a cell culture medium separation and purification device, which solves the problem of low separation and purification efficiency in existing cell culture medium separation and purification devices mentioned in the background art.

[0005] To achieve the above objectives, this utility model is implemented through the following technical solution: a cell culture medium separation and purification device, comprising two sets of horizontally opposite support frames;

[0006] The upper end of the support frame is rotatably connected to a flipping shaft, and a flipping braking mechanism for driving the flipping shaft is provided on one side of the support frame.

[0007] A tilting frame is provided in the middle of the shaft of the tilting shaft, and a centrifugal purification component is installed at the front end of the support of the tilting frame.

[0008] The centrifugal purification assembly includes a centrifuge tank, with a centrifugal mesh cylinder rotatably connected inside the tank body, and a centrifugal braking mechanism for driving the centrifugal mesh cylinder is provided at the upper end of the tank body.

[0009] As a further technical solution of this utility model: the tilting braking mechanism includes a brake housing installed on the support frame along the tilting shaft and a synchronous pulley B installed at one end of the tilting shaft. A tilting motor is installed inside the brake housing. The output end of the tilting motor is provided with a synchronous pulley A opposite to the synchronous pulley B, and the synchronous pulley A and the synchronous pulley B are connected by a synchronous belt drive.

[0010] As a further technical solution of this utility model: the upper and lower ends of the support of the tilting frame are provided with ring sleeves for the centrifuge tank.

[0011] As a further technical solution of this utility model: the centrifugal braking mechanism includes a centrifugal motor installed on the upper end of the centrifugal tank and a differential pulley B installed on the top of the centrifugal mesh cylinder. The output end of the centrifugal motor is provided with a differential pulley A opposite to the differential pulley B, and the differential pulley A and the differential pulley B are connected by differential belt drive.

[0012] As a further technical solution of this utility model: the lower end of the centrifuge tank is provided with a first rotating seat for supporting the centrifuge mesh cylinder, and the upper end of the centrifuge tank is provided with a second rotating seat for supporting the centrifuge mesh cylinder.

[0013] As a further technical solution of this utility model: a liquid injection valve is installed at the upper end of the centrifuge mesh cylinder, a first drain valve is installed at the bottom of the centrifuge tank, and a second drain valve is installed at the bottom of the centrifuge tank opposite to the centrifuge mesh cylinder.

[0014] This invention provides a cell culture medium separation and purification device, which has the following advantages compared with the prior art:

[0015] 1. The cell culture medium separation and purification device designed in this paper uses a flipping braking mechanism as the flipping braking source to drive the centrifuge tank to flip and rotate, flexibly switching it between vertical, inclined and horizontal states. At the same time, the centrifuge mesh cylinder is driven to rotate centrifugally based on the centrifugation braking mechanism, so as to carry out separation and purification of cell culture medium under different states. Its separation and purification is more diversified, the centrifugation separation state is more variable, and the separation efficiency is more efficient.

[0016] 2. The cell culture medium separation and purification device designed in this paper utilizes the rotation combination between the centrifuge tank and the centrifuge mesh cylinder, and with the conduction of the injection valve and two sets of drainage valves, it can feed, separate and purify the cell culture medium in a closed feeding manner, without the need to frequently turn on the separation and purification device, thus minimizing the impact of external environmental food on the cell culture medium contamination. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of a cell culture medium separation and purification device.

[0018] Figure 2 This is a schematic diagram of the tilting braking mechanism in a cell culture medium separation and purification device.

[0019] Figure 3 This is a schematic diagram of the centrifugal braking mechanism in a cell culture medium separation and purification device.

[0020] Figure 4 This is a schematic diagram of the assembly of a centrifuge mesh in a cell culture medium separation and purification device.

[0021] In the diagram: 1. Support frame; 2. Tilting shaft; 3. Tilting frame; 4. Ring sleeve; 5. Brake housing; 6. Centrifuge tank; 7. Tilting motor; 8. Synchronous pulley A; 9. Synchronous belt; 10. Synchronous pulley B; 11. First rotating seat; 12. Second rotating seat; 13. Centrifuge screen; 14. Centrifuge motor; 15. Differential pulley A; 16. Differential belt; 17. Differential pulley B; 18. Injection valve; 19. First drain valve; 20. Second drain valve. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0023] Please see Figure 1-4 This utility model provides a technical solution for a cell culture medium separation and purification device: A cell culture medium separation and purification device includes two horizontally opposite support frames 1. The upper end of the support frame 1 is rotatably connected to a flip shaft 2, and a flip braking mechanism for driving the flip shaft 2 is provided on one side of the support frame 1. The flip braking mechanism includes a brake housing 5 installed on the support frame 1 along the shaft direction of the flip shaft 2 and a synchronous pulley B10 installed at one end of the shaft of the flip shaft 2. A flip motor 7 is installed inside the housing of the brake housing 5. A synchronous pulley A8 is provided at the output end of the flip motor 7, which is opposite to the synchronous pulley B10. The synchronous pulley A8 and the synchronous pulley B10 are connected by a synchronous belt 9. By controlling the operation of the flip motor 7, the synchronous pulley A8 is driven to rotate. The synchronous pulley B10 is driven to rotate by the intermediate transmission of the synchronous belt 9, which in turn drives the flip shaft 2 to rotate, thus driving the centrifuge tank 6 to flip.

[0024] The upper and lower ends of the support of the flipping frame 3 are equipped with ring sleeves 4 that encircle the centrifuge tank 6. When the flipping shaft 2 rotates, the centrifuge tank 6 is driven to rotate synchronously based on the arm force support combination of the flipping frame 3 and the ring sleeve 4, so as to flexibly switch between vertical, tilted and horizontal states, and perform separation and purification of cell culture medium under different states.

[0025] A tilting frame 3 is provided in the middle of the shaft of the tilting shaft 2, and a centrifugal purification component is installed at the front end of the support of the tilting frame 3. The centrifugal purification component includes a centrifugal tank 6. A centrifugal mesh cylinder 13 is rotatably connected inside the tank body of the centrifugal tank 6. A first rotating seat 11 supporting the centrifugal mesh cylinder 13 is provided at the lower end of the tank body of the centrifugal tank 6, and a second rotating seat 12 supporting the centrifugal mesh cylinder 13 is provided at the upper end of the tank body of the centrifugal tank 6. By utilizing the rotational support of the first rotating seat 11 and the second rotating seat 12, the centrifugal mesh cylinder 13 is kept in a rotating state to perform centrifugal separation.

[0026] The centrifuge tank 6 is equipped with a centrifugal braking mechanism at the upper end of the tank body to drive the centrifugal mesh cylinder 13. The centrifugal braking mechanism includes a centrifugal motor 14 installed at the upper end of the centrifuge tank 6 and a differential pulley B17 installed at the top of the centrifugal mesh cylinder 13. The output end of the centrifugal motor 14 is equipped with a differential pulley A15 opposite to the differential pulley B17. The differential pulley A15 and the differential pulley B17 are connected by a differential belt 16. By controlling the operation of the centrifugal motor 14, the differential pulley A15 is driven to rotate. The differential pulley B17 is driven to rotate by the intermediate transmission of the differential belt 16, which in turn drives the centrifugal mesh cylinder 13 to rotate. As a centrifugal separation component, it performs separation and purification of cell culture medium.

[0027] A liquid injection valve 18 is installed at the upper end of the centrifuge mesh cylinder 13, and a first drain valve 19 is installed at the bottom of the centrifuge tank 6. A second drain valve 20 is installed at the bottom of the centrifuge tank 6, which is connected to the centrifuge mesh cylinder 13. By connecting the liquid injection valve 18 to the centrifuge mesh cylinder 13, the cell culture medium can be directly injected into the centrifuge mesh cylinder 13 for separation and purification. During the subsequent separation and purification process, the first drain valve 19 is used to discharge the purified clear liquid. After the subsequent separation and purification is completed, the second drain valve 20 is connected to the centrifuge mesh cylinder 13 to discharge the purified turbid liquid. It has good closed separation and purification characteristics, and does not require frequent opening of end caps and other components, thus reducing the impact of external environmental factors.

[0028] The working principle of this utility model is as follows: When using the separation and purification device to separate and purify cell culture medium, the cell culture medium is directly injected into the centrifuge mesh cylinder 13 through the injection valve 18 for separation and purification. At the same time as the injection is completed, the injection valve 18 is closed, and then the centrifuge motor 14 is controlled to work, driving the combination of differential pulley A15, differential belt 16, and differential pulley B17 to rotate, which in turn drives the centrifuge mesh cylinder 13 to rotate, serving as a centrifugal separation component to separate and purify the cell culture medium.

[0029] Furthermore, while separating and purifying the cell culture medium, the flipping motor 7 is controlled to work, driving the combination of synchronous pulley A8, synchronous belt 9, and synchronous pulley B10 to rotate, which in turn drives the flipping shaft 2 to rotate. Based on the arm force support combination of the flipping frame 3 and the ring sleeve 4, the centrifuge tank 6 is driven to flip synchronously, flexibly switching between vertical, inclined, and horizontal states, so as to carry out separation and purification of the cell culture medium under different states.

[0030] During the separation and purification process of cell culture medium, the purified clear liquid can be discharged by opening the first drain valve 19. After the subsequent separation and purification is completed, the turbid liquid after separation and purification can be discharged by using the second drain valve 20 and the centrifuge mesh cylinder 13. It has good closed separation and purification operation and is less affected by external environmental factors.

[0031] The above description is merely a preferred embodiment of this utility model. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model. Structures, devices, and operating methods not specifically described or explained in this utility model are implemented according to conventional methods in the art, unless otherwise specified or limited.

Claims

1. A cell culture medium separation and purification device, characterized in that, Includes two sets of horizontally opposite support frames (1); The upper end of the support frame (1) is rotatably connected to a flipping shaft (2), and a flipping braking mechanism for driving the flipping shaft (2) is provided on one side of the support frame (1). The rotating shaft (2) has a rotating frame (3) in the middle of its shaft, and a centrifugal purification component is installed at the front end of the support of the rotating frame (3). The centrifugal purification assembly includes a centrifugal tank (6), a centrifugal mesh cylinder (13) is rotatably connected inside the tank body of the centrifugal tank (6), and a centrifugal braking mechanism for driving the centrifugal mesh cylinder (13) is provided at the upper end of the tank body of the centrifugal tank (6).

2. The cell culture medium separation and purification device according to claim 1, characterized in that, The overturning braking mechanism includes a brake housing (5) mounted on a support frame (1) along the axis of the overturning shaft (2) and a synchronous pulley B (10) mounted on one end of the axis of the overturning shaft (2). The overturning motor (7) is installed inside the housing of the brake housing (5). The output end of the overturning motor (7) is provided with a synchronous pulley A (8) opposite to the synchronous pulley B (10), and the synchronous pulley A (8) and the synchronous pulley B (10) are connected by a synchronous belt (9).

3. The cell culture medium separation and purification device according to claim 1, characterized in that, The upper and lower ends of the support of the tilting frame (3) are provided with ring sleeves (4) for the centrifuge tank (6).

4. The cell culture medium separation and purification device according to claim 1, characterized in that, The centrifugal braking mechanism includes a centrifugal motor (14) installed on the upper end of the centrifugal tank (6) and a differential pulley B (17) installed on the top of the centrifugal mesh cylinder (13). The output end of the centrifugal motor (14) is provided with a differential pulley A (15) opposite to the differential pulley B (17), and the differential pulley A (15) and the differential pulley B (17) are connected by a differential belt (16).

5. The cell culture medium separation and purification device according to claim 1, characterized in that, The centrifuge tank (6) has a first rotating seat (11) for supporting the centrifuge mesh cylinder (13) at the lower end of the tank body, and a second rotating seat (12) for supporting the centrifuge mesh cylinder (13) at the upper end of the tank body.

6. The cell culture medium separation and purification device according to claim 1, characterized in that, The centrifuge mesh cylinder (13) is connected to the upper end of the liquid injection valve (18), the centrifuge tank (6) is connected to the bottom end of the tank body of the first drain valve (19), and the bottom of the centrifuge tank (6) is connected to the second drain valve (20) opposite to the centrifuge mesh cylinder (13).

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