Medium-high temperature anaerobic fermentation flora expanding culture device

Abstract

The utility model provides a medium-high temperature anaerobic fermentation flora expanding cultivation device which comprises a fermentation unit and further comprises an expanding cultivation unit, the expanding cultivation unit comprises an expanding cultivation assembly and a recycling assembly which are connected with each other, and the fermentation unit, the recycling assembly and the expanding cultivation assembly are sequentially connected to form a loop. The expanding culture assembly contains expanding culture flora and a mixed auxiliary agent, the expanding culture flora and the mixed auxiliary agent are input into the fermentation unit, and the recovery assembly recovers the mixed auxiliary agent in the fermentation unit. According to the utility model, through the arrangement of the recovery assembly, the auxiliary material contained in the anaerobic fermentation discharged material is recovered, and the auxiliary material enters the fermentation unit through the expanding culture flora of the expanding culture assembly, so that the recovery and reutilization of the auxiliary material are realized.

Description

Technical Field

[0001] This utility model relates to the field of medium- and high-temperature anaerobic fermentation, and in particular to a medium- and high-temperature anaerobic fermentation microbial culture expansion device. Background Technology

[0002] Significant breakthroughs have been achieved in anaerobic fermentation in the mesophilic-thermophilic transition zone (40℃~45℃), which combines the stability of mesophilic anaerobic fermentation with the high efficiency of thermophilic anaerobic fermentation. However, mesophilic-thermophilic anaerobic fermentation has stringent requirements for the abundance and activity of methanogenic bacteria, and current technologies cannot guarantee the enrichment of highly efficient methanogenic bacteria. Methods such as adding inoculants and adjuvants to the fermentation vessel to enrich the microbial community and alleviate acid inhibition suffer from slow fermentation system recovery and rapid loss of inoculants and adjuvants, hindering the widespread adoption of these methods.

[0003] For example, Chinese patent CN115261191A discloses a self-sustaining methanogenic bacterial culture and biogas engineering microbial agent supplementation.

[0004] A vehicle and cultivation method are provided, including a vehicle compartment and an anaerobic bioreactor installed within the vehicle compartment for the enrichment and cultivation of methanogenic bacteria; a mixing tank connected to the anaerobic bioreactor, the mixing tank being used to provide the necessary culture medium for the reaction within the anaerobic bioreactor; a temporary storage tank connected to the anaerobic bioreactor, the temporary storage tank being used to temporarily store a portion of the bio-enhancing agent generated in the reaction within the anaerobic bioreactor; and a gas-fired power generation device connected to the anaerobic bioreactor, the gas-fired power generation device using the biogas generated in the reaction within the anaerobic bioreactor to generate electricity. The self-powered methanogenic bacteria cultivation and biogas engineering agent supply vehicle of the above-mentioned invention application can realize the expansion and cultivation of methanogenic bacteria. Through the cooperation of the mixing tank and the temporary storage tank, the culture medium required for expansion is delivered to the anaerobic bioreactor, and the expanded bacteria are then input into the fermentation tank in the biogas project. However, the microbial community introduced into the fermenter after expansion culture contains materials such as culture medium and auxiliary agents. After anaerobic fermentation, these auxiliary materials will be lost along with the biogas slurry and biogas residue, which can easily lead to waste of resources. Utility Model Content

[0005] To address the problem that the auxiliary materials contained in the current cultured microorganisms are difficult to recover during the fermentation discharge process, this invention proposes a microbial culture expansion device that can ensure the expansion of medium- and high-temperature anaerobic fermentation microorganisms while recovering and reusing the auxiliary materials and sludge containing anaerobic microorganisms.

[0006] To achieve the above objectives, this utility model proposes:

[0007] A medium- and high-temperature anaerobic fermentation microbial community expansion device includes a fermentation unit and an expansion unit. The expansion unit includes an expansion component and a recovery component connected to each other. The fermentation unit, the recovery component, and the expansion component are sequentially connected to form a loop. The expansion component contains expansion microbial communities and mixed adjuvants. The expansion microbial communities and the mixed adjuvants are input into the fermentation unit. The recovery component recovers the mixed adjuvants from the fermentation unit. The expansion component is used to expand the microbial community required for medium- and high-temperature anaerobic fermentation. The expansion component contains expansion microbial communities with high activity in medium- and high-temperature anaerobic fermentation. The mixed adjuvants are used to improve expansion efficiency. The recovery component is used to recover the mixed adjuvants from the fermentation unit. The fermentation unit, the recovery component, and the expansion component are sequentially connected to form a loop. By recovering the adjuvant materials through the recovery component and re-inputting the recovered adjuvant materials into the loop, the purpose of recovering and utilizing the adjuvant materials and part of the microbial community is achieved.

[0008] The propagation unit includes an adjuvant mixing component containing a mixed adjuvant, which includes a compound microbial agent, adjuvants, and a magnetically mediated material. A first feeding component is disposed between the adjuvant mixing component and the propagation unit. The adjuvant mixing component is used to mix the materials constituting the mixed adjuvant, including the compound microbial agent, adjuvants, and magnetically mediated material.

[0009] The cultured microbiota contains methanogens and syntrophic acetic acid oxidizing bacteria.

[0010] The compound bacterial agent is made from expanded bacterial cultures, and the surface magnetic strength of the magnetic mediating material is at least 4500 Gs.

[0011] The recycling component includes a magnetic separation device that separates the magnetically mediated material. After the magnetically mediated material undergoes medium- and high-temperature anaerobic fermentation in the fermentation unit, it forms biogas, biogas slurry, and anaerobic sludge. The anaerobic sludge contains the magnetically mediated material. The magnetic separation device separates the anaerobic sludge containing the magnetically mediated material and some additives, achieving the purpose of recycling.

[0012] The expansion component includes an adjuvant inlet and an expansion outlet. The adjuvant inlet is connected to the adjuvant mixing component, and the expansion outlet is connected to the fermentation unit. It also includes a recovery inlet, which is connected to the recovery component.

[0013] The fermentation unit includes a fermentation component and a buffer component. The propagation component is connected to the fermentation component, and the recovery component is connected to the buffer component. The fermentation component serves as the main anaerobic fermentation vessel, while the buffer component performs post-fermentation and sedimentation of the biogas slurry in the fermentation component.

[0014] A second feeding component is provided between the expansion component and the fermentation component, a third feeding component is provided between the fermentation component and the buffer component, a fourth feeding component is provided between the buffer component and the recovery component, and a fifth feeding component is provided between the recovery component and the expansion component.

[0015] The propagation assembly also includes a temperature control assembly, which comprises a temperature measuring component located inside the propagation assembly and a heat exchange component connected to the outside of the propagation assembly. The heat exchange component controls the temperature of the propagation assembly at 43.5℃~44.5℃. The temperature control assembly includes a temperature measuring component disposed inside the propagation assembly and a heat exchange component disposed outside the propagation assembly. The temperature measuring component detects the temperature inside the propagation assembly, and the heat exchange component outside the propagation assembly controls the temperature of the propagation assembly, thereby maintaining the temperature of the propagation assembly at 43.5℃~44.5℃.

[0016] The beneficial effects of this utility model are:

[0017] The expansion component expands the microbial community required for medium- and high-temperature anaerobic fermentation in the fermentation unit. During the expansion process, a mixed additive containing magnetic mediating materials is added to the expansion component. The anaerobic sludge containing magnetic mediating materials in the fermentation unit is separated by a magnetic separation device in the recovery component, achieving the purpose of resource recycling and avoiding excessive loss of additive materials. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of a medium- and high-temperature anaerobic fermentation microbial culture expansion device.

[0019] Figure 2 This is a schematic diagram of the propagation component.

[0020] Icon labels:

[0021] 1. Fermentation unit; 2. Expansion unit; 11. Fermentation component; 12. Buffer component; 21. Expansion component; 22. Recovery component; 23. Additive mixing component; 31. First feed component; 32. Second feed component; 33. Third feed component; 34. Fourth feed component; 35. Fifth feed component; 211. Additive inlet; 212. Expansion outlet; 213. Recovery inlet; 214. Temperature control component; 215. Temperature measuring component; 216. Heat exchange component. Detailed Implementation

[0022] The following specific embodiments illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification.

[0023] This utility model provides a medium- and high-temperature anaerobic fermentation microbial culture expansion device. The preferred embodiments of the medium- and high-temperature anaerobic fermentation microbial culture expansion device are described below.

[0024] Example 1

[0025] like Figure 1 As shown, the medium- and high-temperature anaerobic fermentation microbial community expansion device includes a fermentation unit 1 and an expansion unit 2. Fermentation unit 1 is used for medium- and high-temperature anaerobic fermentation of organic materials, while expansion unit 2 is used to expand the anaerobic microbial community required in fermentation unit 1 and periodically introduce the expanded microbial community into fermentation unit 1 to ensure that fermentation unit 1 remains in a state of high-efficiency fermentation. Fermentation unit 1 includes a fermentation component 11 and a buffer component 12. Fermentation component 11 serves as the main fermentation vessel for organic materials, and a mixer is installed on its top to agitate the organic materials inside fermentation component 11, ensuring thorough fermentation. Buffer component 12 is used for post-fermentation and sedimentation of the biogas slurry produced in fermentation component 11. The clear liquid produced after sedimentation enters subsequent processing steps, while the concentrated liquid enters expansion unit 2. A third feeding component 33 is provided between fermentation component 11 and buffer component 12.

[0026] The expansion unit 2 includes an expansion component 21 and a recovery component 22. The expansion component 21 contains expansion microbial communities, which are composed of microorganisms with high activity in medium- and high-temperature anaerobic fermentation, including methanogens and symbiotic acetic acid oxidizing bacteria. The expansion component 21 also includes an auxiliary agent mixing component 23, which contains a mixed auxiliary agent that can improve the proliferation efficiency of the microbial communities in the expansion component 21. The mixed auxiliary agent consists of a compound microbial agent, an auxiliary agent, and a magnetic mediating material. The compound microbial agent is made from the expansion microbial communities, and the auxiliary agent is made from an EDTA nutrient solution containing trace elements such as iron, zinc, cobalt, nickel, copper, and molybdenum. The auxiliary agent mixing component 23 supplements the mixed auxiliary agent into the expansion component 21, which then proliferates and cultivates the expansion microbial communities and periodically replenishes them into the fermentation component 11. A first feeding component 31 is provided between the expansion component 21 and the auxiliary agent mixing component 23, and a second feeding component 32 is provided between the expansion component 21 and the fermentation component 11. The recycling component 22 has a magnetic separation device that can separate anaerobic sludge containing magnetically mediated materials. The surface magnetic strength of the magnetically mediated materials is 4500 Gs. The magnetically mediated materials are magnetic biochar with a C:Fe mass ratio of 3:1. Magnetic biochar can improve the methanogenesis rate of anaerobic fermentation and increase the cumulative methane yield.

[0027] like Figure 2As shown, the expansion component 21 includes an auxiliary agent inlet 211, an expansion outlet 212, and a recovery inlet 213. The auxiliary agent inlet 211 is connected to the auxiliary agent mixing component 23 for inputting mixed auxiliary agents; the expansion outlet 212 is connected to the fermentation component 11 for supplementing the expansion microbial community to the fermentation component 11; and the recovery inlet 213 is connected to the recovery component 22 for supplementing the expansion component 21 with the sludge containing magnetic mediating material separated from the recovery component 22.

[0028] The expansion component 21 also includes a temperature control component 214, which controls the temperature of the expansion component 21 to ensure the microbial community within it is in a state of efficient proliferation. The temperature control component 214 includes a temperature sensing component 215 and a heat exchange component 216. The temperature sensing component 215 is located on the inner side of the expansion component 21 to detect the internal temperature. The heat exchange component 216 is connected to the outside of the expansion component 21 and works in conjunction with the temperature sensing component 215 to control the temperature. The temperature control component 214 maintains the temperature of the expansion component 21 between 43.5℃ and 44.5℃. This temperature range is considered to have high efficiency in medium- and high-temperature anaerobic fermentation, within which microbial communities with high medium- and high-temperature anaerobic fermentation activity proliferate efficiently. Considering the volume of the expansion component 21, two temperature sensing components 215 are vertically positioned on the side of the expansion component 21, one above the other. Combined with stirring by a mixer located at the top of the expansion component 21, the temperature within the expansion component 21 is relatively uniform. The heat exchange component 216 is installed on the outer side of the expansion component 21 in the form of a coil. The heat exchange component 216 can be connected to equipment such as a hot water storage tank for temperature control.

[0029] The following describes the workflow of a medium-high temperature anaerobic fermentation microbial culture expansion device using kitchen waste as raw material:

[0030] Pre-treated kitchen waste forms an organic slurry and enters the fermentation unit 11. The organic slurry undergoes medium- and high-temperature anaerobic fermentation in the fermentation unit 11. During this process, a mixer stirs the organic material, and the fermentation produces biogas and biogas slurry. The biogas is collected and can be used for heating and power generation. The biogas slurry enters the buffer unit 12 through the third feed unit 33. The biogas slurry entering the buffer unit 12 contains some biogas residue, some auxiliary materials, and magnetic mediator materials. The buffer unit 12 performs post-fermentation and sedimentation on the biogas slurry. The sedimented sludge enters the subsequent treatment, and the sedimented sludge enters the recycling unit 22 through the fourth feed unit 34. The magnetic separation device in the recycling unit 22 separates the magnetic mediator materials containing anaerobic sludge, and then enters the expansion unit 21 through the fifth feed unit 35 for recycling. The separated sludge enters the subsequent treatment. The expansion component 21 expands the medium- and high-temperature anaerobic fermentation bacteria required by the fermentation component 11. The auxiliary agent mixing component 23 mixes the compound bacterial agent, auxiliary agent and magnetic mediator material required for the expansion bacteria and replenishes them to the expansion component 21 regularly. The temperature measuring component 215 detects the internal temperature of the expansion component 21 and, together with the heat exchange component 216, controls the temperature of the expansion component 21 at 43.5℃~44.5℃, so that the expansion bacteria are in a state of high proliferation efficiency. The expansion component 21 periodically replenishes the expansion bacteria to the fermentation component 11 through the second feeding component 32.

[0031] Example 2

[0032] The medium- and high-temperature anaerobic fermentation microbial community expansion device includes a fermentation unit 1 and an expansion unit 2. Fermentation unit 1 is used for medium- and high-temperature anaerobic fermentation of organic materials. Expansion unit 2 is used to expand the anaerobic microbial community required in fermentation unit 1 and periodically introduces the expanded microbial community into fermentation unit 1 to ensure that fermentation unit 1 remains in a state of high-efficiency fermentation. Fermentation unit 1 includes a fermentation component 11 and a buffer component 12. Fermentation component 11 serves as the main fermentation vessel for organic materials and is equipped with a mixer on top to agitate the organic materials inside, ensuring thorough fermentation. Buffer component 12 is used for post-fermentation and sedimentation of the biogas slurry produced in fermentation component 11. The clear liquid produced after sedimentation enters subsequent processing steps, while the concentrated liquid enters expansion unit 2. A third feeding component 33 is provided between fermentation component 11 and buffer component 12.

[0033] The expansion unit 2 includes an expansion component 21 and a recovery component 22. The expansion component 21 contains expansion microbial communities, which are composed of microorganisms with high activity in medium- and high-temperature anaerobic fermentation, including methanogens and symbiotic acetic acid oxidizing bacteria. The expansion component 21 also includes an auxiliary agent mixing component 23, which contains a mixed auxiliary agent that can improve the proliferation efficiency of the microbial communities in the expansion component 21. The mixed auxiliary agent consists of a compound microbial agent, an auxiliary agent, and a magnetic mediating material. The compound microbial agent is made from the expansion microbial communities, and the auxiliary agent is made from an EDTA nutrient solution containing trace elements such as iron, zinc, cobalt, nickel, copper, and molybdenum. The auxiliary agent mixing component 23 supplements the mixed auxiliary agent into the expansion component 21, which then proliferates and cultivates the expansion microbial communities and periodically replenishes them into the fermentation component 11. A first feeding component 31 is provided between the expansion component 21 and the auxiliary agent mixing component 23, and a second feeding component 32 is provided between the expansion component 21 and the fermentation component 11. The recycling component 22 has a magnetic separation device that can separate anaerobic sludge containing magnetically mediated materials. The surface magnetic strength of the magnetically mediated materials is 4500 Gs. The magnetically mediated materials are magnetic biochar with a C:Fe mass ratio of 3:1. Magnetic biochar can improve the methanogenesis rate of anaerobic fermentation and increase the cumulative methane yield.

[0034] The expansion component 21 includes an auxiliary agent inlet 211, an expansion outlet 212, and a recovery inlet 213. The auxiliary agent inlet 211 is connected to the auxiliary agent mixing component 23 for inputting mixed auxiliary agents. The expansion outlet 212 is connected to the fermentation component 11 for supplementing the expansion microbial community to the fermentation component 11. The recovery inlet 213 is connected to the recovery component 22 for supplementing the expansion component 21 with the sludge containing magnetic mediating material separated from the recovery component 22.

[0035] The expansion component 21 also includes a temperature control component 214, which controls the temperature of the expansion component 21 to ensure the microbial community within it is in a state of efficient proliferation. The temperature control component 214 includes a temperature sensing component 215 and a heat exchange component 216. The temperature sensing component 215 is located on the inner side of the expansion component 21 to detect the internal temperature. The heat exchange component 216 is connected to the outside of the expansion component 21 and works in conjunction with the temperature sensing component 215 to control the temperature. The temperature control component 214 maintains the temperature of the expansion component 21 between 43.5℃ and 44.5℃. This temperature range is considered to have high efficiency in medium- and high-temperature anaerobic fermentation, within which microbial communities with high medium- and high-temperature anaerobic fermentation activity proliferate efficiently. Considering the volume of the expansion component 21, two temperature sensing components 215 are vertically positioned on the side of the expansion component 21, one above the other. Combined with stirring by a mixer located at the top of the expansion component 21, the temperature within the expansion component 21 is relatively uniform. The heat exchange component 216 is installed on the outer side of the expansion component 21 in the form of a coil. The heat exchange component 216 can be connected to equipment such as a hot water storage tank for temperature control.

[0036] The following describes the workflow of a medium-high temperature anaerobic fermentation microbial culture expansion device using cow dung as raw material:

[0037] Pre-treated cow manure enters fermentation component 11, where it undergoes medium-to-high temperature anaerobic fermentation. During this process, a mixer agitates the cow manure, producing biogas and biogas slurry. The biogas is collected and can be used for heating and power generation. The biogas slurry enters buffer component 12 through third feed component 33. The biogas slurry entering buffer component 12 contains some biogas residue, some auxiliary materials, and magnetic mediator materials. Buffer component 12 performs post-fermentation and sedimentation on the biogas slurry. The precipitated sludge enters subsequent treatment, and the precipitated sludge enters recovery component 22 through fourth feed component 34. The magnetic separation device in recovery component 22 separates the magnetic mediator materials containing anaerobic sludge, which then enters expansion component 21 through fifth feed component 35 for recycling. The separated sludge enters subsequent treatment. The expansion component 21 expands the medium- and high-temperature anaerobic fermentation bacteria required by the fermentation component 11. The auxiliary agent mixing component 23 mixes the compound bacterial agent, auxiliary agent and magnetic mediator material required for the expansion bacteria and replenishes them to the expansion component 21 regularly. The temperature measuring component 215 detects the internal temperature of the expansion component 21 and, together with the heat exchange component 216, controls the temperature of the expansion component 21 at 43.5℃~44.5℃, so that the expansion bacteria are in a state of high proliferation efficiency. The expansion component 21 periodically replenishes the expansion bacteria to the fermentation component 11 through the second feeding component 32.

[0038] Biogas projects based on medium- and high-temperature anaerobic fermentation have high fermentation efficiency, but they suffer from slow growth of anaerobic microorganisms and rapid loss of auxiliary materials. Therefore, it is particularly important to set up a device for expanding the microbial community in the fermentation system. In the existing technology, magnetic biochar is added to improve the activity of the microbial community and fermentation efficiency, but magnetic biochar is easily lost with the output. This utility model, through the setting of a recovery component, can recover and reuse the magnetic biochar in the biogas slurry produced by anaerobic fermentation. At the same time, the beneficial microbial community on the surface and in the crevices of the magnetic biochar, as well as the auxiliary materials in the anaerobic sludge, can be recovered and then added to the expansion component. When the expansion component delivers the expanded microbial community to the fermentation unit, it re-enters the fermentation unit.

Claims

1. A medium-to-high temperature anaerobic fermentation microbial culture propagation device, comprising a fermentation unit, characterized in that, It also includes an expansion unit, which includes an expansion component and a recovery component connected to each other, a fermentation unit, and the recovery component and the expansion component are sequentially connected to form a loop. The expansion component contains expansion microorganisms and mixed additives. The expansion microorganisms and the mixed additives are input into the fermentation unit, and the recovery component recovers the mixed additives in the fermentation unit.

2. The medium-high temperature anaerobic fermentation microbial community expansion device according to claim 1, characterized in that, The expansion unit includes an additive mixing component containing mixed additives, and a first feeding component is provided between the additive mixing component and the expansion unit.

3. The medium-high temperature anaerobic fermentation microbial culture expansion device according to claim 2, characterized in that, The recycling assembly has a magnetic separation device for separating magnetically mediated materials.

4. The medium-high temperature anaerobic fermentation microbial community expansion device according to claim 3, characterized in that, The surface magnetic force of the magnetic mediator is at least 4500 Gs.

5. The medium-high temperature anaerobic fermentation microbial culture expansion device according to claim 2, characterized in that, The expansion component includes an adjuvant inlet and an expansion outlet. The adjuvant inlet is connected to the adjuvant mixing component, and the expansion outlet is connected to the fermentation unit. It also includes a recovery inlet, which is connected to the recovery component.

6. A medium-to-high temperature anaerobic fermentation microbial culture expansion device according to any one of claims 1 to 5, characterized in that, The fermentation unit includes a fermentation component and a buffer component. The propagation component is connected to the fermentation component, and the recovery component is connected to the buffer component.

7. The medium-high temperature anaerobic fermentation microbial community expansion device according to claim 6, characterized in that, A second feeding component is provided between the expansion component and the fermentation component, a third feeding component is provided between the fermentation component and the buffer component, a fourth feeding component is provided between the buffer component and the recovery component, and a fifth feeding component is provided between the recovery component and the expansion component.

8. The medium-high temperature anaerobic fermentation microbial community expansion device according to claim 6, characterized in that, The propagation component also includes a temperature control component, which comprises a temperature measuring component located inside the propagation component and a heat exchange component connected to the outside of the propagation component. The temperature control component controls the temperature of the propagation component at 43.5℃~44.5℃.

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