Microorganism experiment measuring device

Abstract

The utility model belongs to the technical field of microorganisms, and relates to a microorganism experiment measuring device, which is used for more efficiently measuring the growth condition of microorganisms during a microorganism experiment and avoiding errors caused by excessive operations and structures. The culture dish comprises the culture dish cover, the culture dish body, the oxford cup, the measuring disc and the connecting column, the oxford cup is combined with the culture dish cover, liquid leakage pollution can be prevented while the structure of the device is simplified, the experiment success rate and accuracy are improved, the microorganism growth result can be simply and rapidly measured by utilizing the rotatable anti-slip measuring disc, and the test efficiency is improved. Height scale marks are also arranged on the culture dish body, so that the addition of the culture medium can be more accurately controlled, and the accuracy of experimental data is ensured; the device is scientific and reasonable in overall structural design, convenient to use, simple in structure, high in operation success rate and accuracy, low in manufacturing cost and easy to produce and use on a large scale.

Description

Technical Field:

[0001] This utility model belongs to the field of microbial technology and relates to a microbial experimental measurement device, which is used to measure the growth of microorganisms more efficiently during microbial experiments and avoid errors caused by excessive operation and structure. Background technology:

[0002] Microorganisms encompass a wide range of beneficial and harmful species, including bacteria, viruses, and fungi. Their applications are extensive, spanning food, medicine, industry, agriculture, environmental protection, sports, and many other fields, and are closely related to human life. In recent years, with advancements in science and technology, research on the industrialization of microorganisms has deepened, with microbial experiments playing a crucial role in the development of their industrial applications. The growth-promoting, disease-resistant, and enzyme-producing functions of microorganisms, as an important part of industrialization, require extensive microbial experiments. The main experimental methods involve inoculating relevant microorganisms into different screening media and observing the appearance of a clear zone to verify whether the microorganisms possess enzyme-producing or growth-promoting functions. Alternatively, pathogens and microorganisms can be co-inoculated, and the appearance of an antagonistic zone indicates the inhibitory effect of the microorganisms on the pathogens. The Oxford cup is an experimental device used in microbiology experiments for antimicrobial experiments. Its main function is to allow the sample to slowly diffuse into the surrounding culture medium after being added to the cup, thereby observing its inhibitory effect on the growth of specific microorganisms on the medium. This experimental method is simple, intuitive, and provides reliable results, thus holding an important position in microbiological research and drug development. The existing common operating method requires placing the Oxford cup vertically on the surface of the culture medium and gently pressing it to ensure that there are no gaps between it and the petri dish. The test microorganism is then inoculated into the Oxford cup on the culture medium in the form of fermentation broth before subsequent experiments are carried out. However, this step is very prone to errors, which can lead to leakage of the microbial fermentation broth from the Oxford cup and contamination.

[0003] In the prior art, Chinese patent CN214457967U discloses a disposable culture dish for laboratory drug antibacterial activity detection, including a lid and a bottom. A sample loading component is detachably connected to the bottom of the dish. The sample loading component includes a ring support, a linear support, and Oxford cups. The end of the linear support is fixed to the side wall of the ring support. The linear support and the ring support are on the same horizontal plane. The bottom of the ring support is fixedly connected to the end of the Oxford cups. The Oxford cups are perpendicular to the ring support. The number of Oxford cups is 3 to 5. The length of the Oxford cups is equal to the inner height of the bottom of the dish. A dropper hole is opened on the ring support and communicates with the Oxford cups. This novel experimental device connects the Oxford cups together through the ring support and the linear support to form a sample loading component. The sample loading component cooperates with the bottom of the dish, which can effectively improve stability and prevent the Oxford cups from tipping over.

[0004] Chinese patent CN212504887U discloses an Oxford cup, including an Oxford cup body and an Oxford cup connecting assembly. The connecting assembly includes at least three fixed cylinders arranged in a ring at equal angles, connected together by support rods. Each support rod has a long groove in its center, within which a tubular level is fixed. The Oxford cup body is coaxially fixed inside the fixed cylinder. This device includes a level detection mechanism; when the bubbles in the three tubular level are all in the center position, the Oxford cup is vertical. The Oxford cup can then be slowly placed on the culture medium. The operation is simple and reduces operational difficulty. Because multiple Oxford cups are connected as one unit, it prevents the Oxford cup from tipping over.

[0005] Chinese patent CN215162680U discloses a culture device for microbial resistance testing. The device includes: a culture dish for holding the culture medium, and a grid-like support frame placed inside the culture dish to fix Oxford cups. The support frame consists of a quadrilateral grid of intersecting horizontal and vertical bars, the grid size of which is adapted to the outer diameter of the Oxford cups, used to fix the Oxford cups inside the culture dish. The support frame is placed directly inside the culture dish via several feet at the bottom of the support; or it is fixed to the edge of the culture dish by several clamps; or the ends of several horizontal and / or vertical bars of the support frame extend downwards and outwards, thereby hooking and fixing to the edge of the culture dish. This application, by adding structural components such as the support frame, greatly improves the stability of the Oxford cups and effectively solves problems such as culture dish transfer during testing, or Oxford cup tipping or drifting that may occur during experimental operations.

[0006] However, the aforementioned existing technologies all require the use of additional structures or equipment to fix the Oxford cup, resulting in relatively complex structures. These additional fixing devices require rigorous sterilization, increasing operation time and the risk of contamination, leading to a lower experimental success rate. Through research and analysis, the inventors found that no existing technology discloses a rapid measurement device that combines an Oxford cup-like device with a cap-like structure to prevent leakage and contamination, and utilizes a rotatable, non-slip measuring disc for simple and rapid measurement of microbial growth results. Therefore, inventing a microbial experimental measurement device can overcome the shortcomings of existing technologies, save manufacturing and usage costs, simplify operation steps, reduce operational difficulty, increase experimental success rate, and provide convenience and speed. Summary of the Invention:

[0007] The purpose of this invention is to overcome the shortcomings of the prior art. Based on the improvement of existing microbial experimental devices, this invention provides a rapid measurement device that combines an Oxford cup-like device with a cap-like structure to prevent leakage and contamination, and uses a rotatable, non-slip measuring disc to perform simple and rapid measurement of microbial growth results. This solves the problems of long operation time and high risk of contamination caused by the need to use extra structures or equipment to fix the Oxford cup in existing operating devices.

[0008] To achieve the above objectives, this utility model provides a microbial experimental measurement device, including a petri dish lid, a petri dish body, Oxford cups, a measuring plate, and a connecting column. The petri dish lid corresponds to the size of the petri dish body, and the depth of the petri dish lid is equal to the depth of the petri dish body, allowing the petri dish lid to cover the petri dish body. One to five Oxford cups are arranged on the circumference of the bottom surface of the petri dish lid, with the center of the bottom surface of the petri dish lid as the center and half the radius of the bottom surface of the petri dish lid as the radius. The upper bottom surface of the Oxford cups is fixedly connected to the bottom surface of the petri dish lid, and the height of the Oxford cups is consistent with the depth of the petri dish body. A connecting column is fixedly connected downward to the center of the bottom surface of the petri dish body. A measuring plate is movably fitted on the outside of the connecting column. The measuring plate is the same size as the bottom surface of the petri dish body, and a circular hole is provided in the center. The measuring plate is fitted under the petri dish body through the circular hole. Diameter graduations are provided on the upper bottom surface of the measuring plate along the radial direction of the measuring plate for measuring the size of the transparent zone or inhibition zone.

[0009] Furthermore, the connecting post below the measuring plate can be detachably connected to a limiting plug. The connecting post is provided with a cylindrical concave hole, and the limiting plug is a cylinder with an outer diameter slightly smaller than the concave hole of the connecting post. One end is provided with a limiting piece that exceeds the bottom surface area of ​​the limiting plug cylinder. The limiting post can be inserted into the concave hole at one end of the connecting post, so that when the measuring plate is sleeved on the connecting post, it can be blocked by the limiting piece and is not easy to fall off.

[0010] Furthermore, the petri dish also has height graduations along its height on its side for measuring the depth of the culture medium.

[0011] Furthermore, the bottom surface of the measuring disk is also provided with micro-protrusions to increase friction and facilitate the rotation of the measuring disk.

[0012] Compared with the prior art, this utility model has the following advantages: (1) Combining the petri dish lid with the Oxford cup simplifies the device structure and effectively reduces the risk of contamination, improves the success rate and accuracy of experiments, and reduces the need for sterilization devices; (2) Setting height graduation lines on the petri dish body enables more precise control of the addition of culture medium and ensures the accuracy of experimental data; (3) Setting a rotatable measuring plate at the bottom of the petri dish body allows for precise and rapid measurement of the microbial reaction zone simply by rotating it, which is convenient, quick, and saves experimental operation time. The measuring plate has micro-protrusions at the bottom, which make it easy to rotate. Its overall structure design is scientific and reasonable, easy to use, simple in structure, has a high success rate and accuracy, low manufacturing cost, and is easy to mass-produce and use. Attached image description:

[0013] Figure 1 This is a schematic diagram of the overall structure and principle of the microbial experimental measurement device involved in this utility model.

[0014] Figure 2 This is a schematic diagram of the disassembled structure of the microbial experimental measurement device involved in this utility model.

[0015] Figure 3 This is a schematic diagram illustrating the structural principle of the limiting plug involved in this utility model.

[0016] The components in the attached diagram are labeled as follows: 1. Petri dish lid, 2. Petri dish body, 3. Oxford cup, 4. Connecting column, 5. Measuring plate, 6. Limiting plug, 7. Diameter scale line, 8. Height scale line. Detailed implementation method:

[0017] The technical solution of this utility model will be further described below with reference to the accompanying drawings and specific embodiments.

[0018] Example 1:

[0019] This embodiment relates to a microbial experimental measurement device, including a petri dish lid 1, a petri dish body 2, Oxford cups 3, a measuring plate 5, and a connecting column 4. The petri dish lid 1 corresponds in size to the petri dish body 2. The inner diameter of the petri dish lid 1 is 0.5 mm larger than the outer diameter of the petri dish body 2, and the depth of the petri dish lid 1 is equal to the depth of the petri dish body 2, so that the petri dish lid 1 can fit exactly on the petri dish body 2. One to five Oxford cups 3 are arranged on the circumference of the bottom surface of the petri dish lid 1 with the center of the bottom surface of the petri dish lid 1 as the center and half the radius of the bottom surface of the petri dish lid 1 as the radius. The upper bottom surface of the Oxford cup 3 is fixedly connected to the lower bottom surface of the petri dish lid 1. The height of the Oxford cup 3 is the same as the depth of the petri dish body 2. A connecting post 4 is fixedly connected downward in the center of the lower bottom surface of the petri dish body 2. A measuring plate 5 is movably fitted on the outside of the connecting post 4. The measuring plate 5 is the same size as the lower bottom surface of the petri dish body 2. A round hole is set in the center. The diameter of the round hole is 0.5mm larger than the outer diameter of the connecting post 4. The measuring plate 5 is fitted under the petri dish body 2 through the round hole. A diameter scale line 7 is set on the upper bottom surface of the measuring plate 5 along the radial direction of the measuring plate 5 for measuring the size of the transparent zone or the inhibition zone.

[0020] In this embodiment, the connecting post 4 below the measuring disk 5 can also be detachably connected to a limiting plug 6. The connecting post 4 is provided with a cylindrical concave hole, and the limiting plug 6 is a cylinder with an outer diameter slightly smaller than the concave hole of the connecting post 4. One end is provided with a limiting piece that exceeds the bottom surface area of ​​the limiting plug 6. The limiting post can be inserted into the concave hole at one end of the connecting post 4, so that when the measuring disk 5 is sleeved on the connecting post 4, it can be blocked by the limiting piece and is not easy to fall off.

[0021] The culture dish 2 involved in this embodiment also has height scale lines 8 on its side along its height direction for measuring the depth of the culture medium.

[0022] The bottom surface of the measuring disk 5 in this embodiment is also provided with micro-protrusions to increase friction and facilitate the rotation of the measuring disk 5.

[0023] The method of using the microbial experimental measuring device involved in this embodiment is as follows:

[0024] S1: Sterilize the petri dish lid 1 and petri dish body 2 using ultraviolet light;

[0025] S2: Pour an appropriate amount of culture medium solution into culture dish 2, adjust the height of the culture medium according to the height scale line 8, and wait for the culture medium to solidify;

[0026] S3: Place the petri dish lid 1 upside down and use a pipette to inject the fermentation broth of the test microorganism into the Oxford cup 3 connected below the petri dish lid 1.

[0027] S4: After aligning the culture dish 2 with the solidified culture medium, invert it onto the culture dish lid 1 so that the culture dish lid 1 and the culture dish 2 fit together perfectly. At this time, the Oxford cup 3 containing the fermentation broth of the test microorganism is vertically inserted into the culture medium.

[0028] S5: Flip the entire combined petri dish lid 1 and petri dish body 2 180° so that the petri dish lid 1 is on top and the petri dish body 2 is on the bottom. At this time, the fermentation broth of the test microorganism in the Oxford cup 3 falls onto the culture medium and comes into contact with it due to gravity.

[0029] S6: Place the entire petri dish in a suitable culture environment and wait for the fermentation broth of the tested microorganism in the culture medium to form a clear zone and an inhibition zone to demonstrate the nutrient utilization of the culture medium or the inhibitory effect on pathogens.

[0030] S7: The diameter of the antibacterial ring is measured by rotating the measuring disk 5 to drive the diameter scale line 7 to move. Since the extension axis of the diameter scale line 7 passes through the center of the measuring disk 5, it can be ensured that the diameter scale line 7 always passes through the center line of the transparent ring and the antibacterial ring during the rotation of the measuring disk 5, so as to improve the convenience and accuracy of the measurement.

[0031] S8: Clean the petri dish lid 1 and petri dish body 2 thoroughly.

Claims

1. A microbial experimental measuring device, characterized in that, The system includes a petri dish lid, a petri dish body, Oxford cups, a measuring plate, and a connecting column. The petri dish lid corresponds to the size of the petri dish body, and the depth of the petri dish lid is equal to the depth of the petri dish body, allowing the petri dish lid to fit over the petri dish body. One to five Oxford cups are arranged on the circumference of the underside of the petri dish lid, with the center of the underside of the petri dish lid as the center and half the radius of the underside of the petri dish lid as the radius. The upper bottom surface of the Oxford cups is fixedly connected to the underside of the petri dish lid, and the height of the Oxford cups is the same as the depth of the petri dish body. A connecting column is fixedly connected downwards to the center of the underside of the petri dish body. A measuring plate is movably fitted on the outside of the connecting column. The measuring plate is the same size as the underside of the petri dish body, and has a circular hole in the center. The measuring plate fits under the petri dish body through the circular hole. Diameter graduations are set on the upper bottom surface of the measuring plate along the radial direction of the measuring plate.

2. The microbial experimental measuring device according to claim 1, characterized in that: The connecting post below the measuring plate can also be detachably connected to a limiting plug. The connecting post is provided with a cylindrical concave hole. The limiting plug is a cylinder with an outer diameter slightly smaller than the concave hole of the connecting post. One end is provided with a limiting piece that exceeds the bottom surface area of ​​the limiting plug cylinder. The limiting post can be inserted into the concave hole at one end of the connecting post.

3. The microbial experimental measuring device according to claim 1, characterized in that: The culture dish also has height scale lines on its side along its height direction.

4. The microbial experimental measuring device according to claim 1, characterized in that: The bottom surface of the measuring plate is also provided with micro-protrusions.

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