Reaction kettle for biological protein enzymolysis reaction

Abstract

The utility model relates to the technical field of biological protein enzymolysis, and discloses a reaction kettle for biological protein enzymolysis reaction, which comprises a kettle body, a rotating motor is arranged above the kettle body, and a sampling mechanism is arranged in the kettle body; the sampling mechanism comprises a plurality of sampling barrels, each sampling barrel is clamped in the kettle body, a sampling opening is formed in the outer surface of each sampling barrel, and a sliding block is slidably connected into each sampling barrel. According to the reaction kettle for the biological protein enzymolysis reaction, the depths of the plurality of sampling barrels extending into the kettle body are different, and height differences exist among the sampling barrels, so that sampling observation work can be carried out at different heights and positions of the kettle body, and the reaction kettle can be used for better sampling and observing biological protein in enzymolysis; a worker can conveniently and accurately judge the biological protein enzymolysis condition in the reaction kettle, the accuracy of biological protein enzymolysis sampling data is improved, and the use reliability of the biological protein enzymolysis reaction kettle is improved.

Description

Technical Field

[0001] This utility model relates to the field of biological proteolysis technology, specifically a reaction vessel for biological proteolysis reaction. Background Technology

[0002] Bioactive proteins, also known as bioactive proteins or bioactive peptides, are a general term for various peptides, ranging from dipeptides to complex linear and cyclic structures, composed of 25 natural amino acids in different compositions and arrangements. They are multifunctional compounds derived from proteins. Enzymes are specific and efficient catalysts; for example, cellulase can catalyze the decomposition of cellulose, and proteases can catalyze the decomposition of proteins. Enzymatic hydrolysis can be used to better extract the active substances in bioactive proteins. To better perform enzymatic hydrolysis of bioactive proteins, a reaction vessel is usually needed to provide a suitable environment for the enzymatic hydrolysis of bioactive proteins.

[0003] The existing utility model with authorization announcement number CN212713561U discloses a novel enzymatic hydrolysis reactor, including a reactor body, an internal cavity, an internal jacket, a water pipe, an inlet tank fixedly connected to one end of the water pipe, and an outlet tank fixedly connected to the other end of the water pipe, with holes on one side of both the inlet tank and the outlet tank.

[0004] Using the above technical solution, the heated cooling water enters the water pipe to heat the inner wall of the reactor body, thereby enabling the device to heat and cool the inner wall of the reactor body. After circulating once, the cooling water inside the water pipe flows into the outlet tank. A return pipe connects the outlet tank and the inlet tank, thus returning the cooling water in the outlet tank to the inlet tank, achieving the effect of reuse. However, with the above technical solution, it is inconvenient to sample and observe the enzymatically hydrolyzed biological proteins in the reactor during use. Sampling can only be done through the discharge port, and biological proteins cannot be sampled from other locations in the reactor. This greatly affects the accuracy of biological enzymatic hydrolysis sampling data, making it difficult for operators to accurately judge the biological enzymatic hydrolysis status in the reactor, and affecting the reliability of the biological enzymatic hydrolysis reactor.

[0005] Therefore, those skilled in the art have provided a reaction vessel for biological enzymatic hydrolysis to solve the problems mentioned in the background art. Utility Model Content

[0006] The purpose of this invention is to provide a reaction vessel for biological enzymatic hydrolysis to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, this utility model provides the following technical solution:

[0008] A reaction vessel for a biological enzymatic hydrolysis reaction includes a vessel body, a rotary motor disposed above the vessel body, and a sampling mechanism disposed inside the vessel body;

[0009] The sampling mechanism includes several sampling cylinders, each of which is snapped into the interior of the vessel body. Each sampling cylinder has a sampling port on its outer surface. A sliding block is slidably connected inside each sampling cylinder. An arc-shaped plate is fixedly connected to one side of each sliding block, and the outer surface of each arc-shaped plate contacts the inner wall of the sampling cylinder. Each arc-shaped plate is located below the sampling port. A compression spring is fixedly connected to the other side of each sliding block, and the other end of each compression spring is fixedly connected to the inner wall of the sampling cylinder. Each compression spring has a telescopic rod inside. The telescopic ends of the rods are all fixedly connected to the other side of the sliding block. The end of each telescopic rod away from the sliding block is fixedly connected to the inner wall of the sampling tube. A sampling frame is slidably connected inside one of the sampling tubes. A collection groove is opened on the upper part of the outer surface of the sampling frame. Two auxiliary blocks are fixedly connected to the outer surface of the sampling frame. Two sliding grooves are opened on the inner wall of each sampling tube. The two auxiliary blocks are slidably connected inside two of the sliding grooves respectively. A pull rod is fixedly connected to the side of the sampling frame away from the sliding block. A baffle is fixedly connected to the left end of the pull rod. A handle is fixedly connected to the left side of the baffle.

[0010] As a further improvement of this utility model: two support frames are fixedly connected to the outer surface of the vessel body, and a positioning plate is fixedly connected to the bottom surface of each support frame.

[0011] As a further embodiment of this utility model: a feeding pipe is fixedly connected to the upper part of the outer surface of the vessel body, and a discharge pipe is fixedly connected to the lower part of the outer surface of the vessel body.

[0012] As a further embodiment of this utility model: a rotating rod is provided inside the vessel body, the power output end of the rotary motor passes through the vessel body and is fixedly connected to the top of the rotating rod, and several stirring blades are fixedly connected to the outer surface of the rotating rod.

[0013] As a further embodiment of this utility model: the bottom end of the rotating rod is rotatably connected to a bearing bracket, and the outer surface of the bearing bracket is fixedly connected to the inner wall of the vessel.

[0014] As a further improvement of this utility model: a reinforcing ring is fixedly connected to the outer surface of each sampling tube, and the outer surface of each reinforcing ring is fixedly connected to the inner wall of the vessel.

[0015] As a further improvement of this utility model: the outside of the vessel body is provided with a number of sealing caps, each of which is snapped onto the outside of the sampling cylinder, and the outer surface of each sealing cap is provided with anti-slip grooves arranged at equal intervals.

[0016] Compared with the prior art, the beneficial effects of this utility model are:

[0017] This invention, by incorporating a pull rod, baffle, and handle, allows the sampling frame to be inserted into the sampling cylinder via an auxiliary block that slides within a sliding groove. The auxiliary block and sliding groove limit the sampling frame's movement, preventing it from shifting. Once the sampling frame moves into the sampling cylinder and contacts the arc-shaped plate, pushing the pull rod further forces the compression spring to contract with the assistance of the telescopic rod until the arc-shaped plate no longer obstructs the sampling port. At this point, the collection groove on the sampling frame is directly below the sampling port, allowing the biological protein inside the vessel to fall into the collection groove for sampling. Then, the sample is directed towards... Pulling the external lever utilizes the thrust provided by the compression spring to push the arc-shaped plate to close the sampling port again. Since the multiple sampling tubes extend into the reactor body to different depths, and there are also height differences between the sampling tubes, it is possible to sample and observe the biological proteins in the enzymatic hydrolysis reaction at different heights and positions within the reactor body. This allows the reactor to better sample and observe the biological proteins in the enzymatic hydrolysis, facilitating accurate judgment of the biological protein hydrolysis status by the staff, increasing the accuracy of biological protein hydrolysis sampling data, and improving the reliability of the biological protein hydrolysis reactor. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the structure of a reaction vessel for a biological proteolytic reaction.

[0019] Figure 2 This is a cross-sectional three-dimensional structural diagram of the reactor body in a biological proteolytic reaction vessel.

[0020] Figure 3 A schematic diagram of the three-dimensional structure of a sampling tube in a reaction vessel for a biological proteolytic reaction;

[0021] Figure 4 This is a cross-sectional three-dimensional structural diagram of a sampling tube in a reaction vessel for a biological proteolytic reaction.

[0022] In the diagram: 1. Kettle body; 2. Rotary motor; 3. Sampling mechanism; 301. Sampling cylinder; 302. Sampling port; 303. Sliding block; 304. Arc plate; 305. Compression spring; 306. Telescopic rod; 307. Sampling frame; 308. Collection trough; 309. Auxiliary block; 310. Sliding groove; 311. Pull rod; 312. Baffle; 313. Handle; 4. Support frame; 5. Positioning plate; 6. Feeding pipe; 7. Discharge pipe; 8. Rotating rod; 9. Stirring blade; 10. Bearing frame; 11. Reinforcing ring; 12. Sealing cover; 13. Anti-slip groove. Detailed Implementation

[0023] Please see Figure 1-4 A reaction vessel for a biological enzymatic hydrolysis reaction includes a vessel body 1, a rotary motor 2 installed above the vessel body 1, a sampling mechanism 3 installed inside the vessel body 1, and two support frames 4 fixedly connected to the outer surface of the vessel body 1. Each support frame 4 has a positioning plate 5 fixedly connected to its bottom surface. The support frames 4 can support and stabilize the position of the vessel body 1. The positioning plate 5, together with the support frames 4 and bolts and other fasteners, can securely fix the device in the working position.

[0024] The sampling mechanism 3 includes several sampling tubes 301, each of which is snapped into the inside of the vessel body 1. Each sampling tube 301 has a sampling port 302 on its outer surface. A feeding pipe 6 is fixedly connected to the upper part of the outer surface of the vessel body 1, and a discharge pipe 7 is fixedly connected to the lower part of the outer surface of the vessel body 1. The feeding pipe 6 allows the operator to easily add the biological protein that needs to be enzymatically hydrolyzed into the vessel body 1, and the discharge pipe 7 can discharge the biological protein after the enzymatic hydrolysis reaction to the outside, ensuring the normal feeding and discharging of the vessel body 1.

[0025] Each sampling cylinder 301 has a sliding block 303 slidably connected inside, and an arc plate 304 is fixedly connected to one side of each sliding block 303. The outer surface of each arc plate 304 is in contact with the inner wall of the sampling cylinder 301. Each arc plate 304 is located below the sampling port 302. A rotating rod 8 is provided inside the vessel body 1. The power output end of the rotary motor 2 passes through the vessel body 1 and is fixedly connected to the top of the rotating rod 8. Several stirring blades 9 are fixedly connected to the outer surface of the rotating rod 8. The power provided by the rotary motor 2, in conjunction with the rotating rod 8, can drive the stirring blades 9 to rotate, thereby stirring the biological protein undergoing enzymatic hydrolysis inside the vessel body 1, so that the biological protein can be fully fused with the reaction enzyme.

[0026] Each sliding block 303 has a compression spring 305 fixedly connected to its other side. The other end of each compression spring 305 is fixedly connected to the inner wall of the sampling cylinder 301. Each compression spring 305 has a telescopic rod 306 inside. The telescopic end of each telescopic rod 306 is fixedly connected to the other side of the sliding block 303. The end of each telescopic rod 306 away from the sliding block 303 is fixedly connected to the inner wall of the sampling cylinder 301. The bottom end of the rotating rod 8 is rotatably connected to a bearing bracket 10. The outer surface of the bearing bracket 10 is fixedly connected to the inner wall of the vessel body 1. The bearing bracket 10 can reduce the swaying of the bottom end of the rotating rod 8 without affecting the normal rotation of the rotating rod 8, thereby improving the rotational stability of the rotating rod 8.

[0027] One of the sampling cylinders 301 has a sampling frame 307 slidably connected inside. The upper part of the outer surface of the sampling frame 307 has a collection groove 308. Two auxiliary blocks 309 are fixedly connected to the outer surface of the sampling frame 307. Two sliding grooves 310 are opened on the inner wall of each sampling cylinder 301. A reinforcing ring 11 is fixedly connected to the outer surface of each sampling cylinder 301. The outer surface of each reinforcing ring 11 is fixedly connected to the inner wall of the vessel body 1. The reinforcing ring 11 can reinforce the sampling cylinder 301, increase the connection between the sampling cylinder 301 and the vessel body 1, and prevent the sampling cylinder 301 from becoming loose.

[0028] Two auxiliary blocks 309 are slidably connected inside two sliding grooves 310 respectively. A pull rod 311 is fixedly connected to the side of the sampling frame 307 away from the sliding block 303. A baffle 312 is fixedly connected to the left end of the pull rod 311. A handle 313 is fixedly connected to the left side of the baffle 312. Several sealing caps 12 are provided on the outside of the vessel body 1. Each sealing cap 12 is snapped onto the outside of the sampling cylinder 301. Anti-slip grooves 13 are arranged at equal intervals on the outer surface of each sealing cap 12. The sealing caps 12 can seal the sampling cylinder 301 that does not need to be sampled, preventing external impurities from entering the sampling cylinder 301 and causing contamination. The anti-slip grooves 13 can increase the convenience of manual operation of the sealing caps 12.

[0029] The working principle of this utility model is as follows: In use, first connect the rotary motor 2 to the power supply. When it is necessary to use the vessel 1 for enzymatic hydrolysis of biological proteins, simply add the biological protein and reaction enzyme into the vessel 1 through the feeding pipe 6. When it is necessary to stir the biological protein in the enzymatic hydrolysis reaction, the power provided by the rotary motor 2, combined with the rotating rod 8, can drive the stirring blade 9 to rotate, thereby stirring the biological protein in the vessel 1 and allowing the biological protein to fully fuse with the reaction enzyme. When it is necessary to sample and observe the biological protein in the vessel 1, the sampling frame 307 can be inserted into the sampling cylinder 301 by using the pull rod 311 in conjunction with the baffle 312 and the handle 313, and by using the auxiliary block 309 to slide inside the sliding groove 310. The limiting effect of the auxiliary block 309 and the sliding groove 310 on the sampling frame 307 can prevent the sampling frame 307 from shifting. After the sampling frame 307 moves into the sampling cylinder 301 and contacts the arc plate 304, continue pushing... Pulling rod 311 forces compression spring 305 to contract with the assistance of telescopic rod 306 until arc plate 304 no longer blocks sampling port 302. At this time, collection groove 308 on sampling rack 307 will be directly below sampling port 302, and then biological protein inside the vessel 1 can fall into collection groove 308 through sampling port 302 for sampling. Then, pull rod 311 outward to push arc plate 304 to close sampling port 302 again using the thrust provided by compression spring 305. Since multiple sampling cylinders 301 extend into the vessel 1 at different depths and there is also a height difference between sampling cylinders 301, sampling and observation of biological protein in enzymatic hydrolysis can be carried out at different heights and positions in the vessel 1. This allows the reactor to better sample and observe biological protein in enzymatic hydrolysis, making it easier for staff to make accurate judgments on the biological protein hydrolysis status in the reactor, increasing the accuracy of biological protein hydrolysis sampling data, and improving the reliability of biological protein hydrolysis reactor.

[0030] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A reaction vessel for a biological proteolytic reaction, comprising a vessel body (1), characterized in that: A rotary motor (2) is provided above the vessel body (1), and a sampling mechanism (3) is provided inside the vessel body (1); The sampling mechanism (3) includes several sampling cylinders (301), each of which is snapped into the interior of the vessel body (1). Each sampling cylinder (301) has a sampling port (302) on its outer surface. A sliding block (303) is slidably connected inside each sampling cylinder (301). An arc-shaped plate (304) is fixedly connected to one side of each sliding block (303). The outer surface of each arc-shaped plate (304) contacts the inner wall of the sampling cylinder (301). Each arc-shaped plate (304) is located below the sampling port (302). A compression spring (305) is fixedly connected to the other side of each sliding block (303). The other end of each compression spring (305) is fixedly connected to the inner wall of the sampling cylinder (301). A telescopic rod (306) is provided inside each compression spring (305). The telescopic ends are all fixedly connected to the other side of the sliding block (303). The end of each telescopic rod (306) away from the sliding block (303) is fixedly connected to the inner wall of the sampling tube (301). A sampling frame (307) is slidably connected inside one of the sampling tubes (301). A collection groove (308) is opened on the upper part of the outer surface of the sampling frame (307). Two auxiliary blocks (309) are fixedly connected to the outer surface of the sampling frame (307). Two sliding grooves (310) are opened on the inner wall of each sampling tube (301). The two auxiliary blocks (309) are slidably connected inside the two sliding grooves (310). A pull rod (311) is fixedly connected to the side of the sampling frame (307) away from the sliding block (303). A baffle (312) is fixedly connected to the left end of the pull rod (311). A handle (313) is fixedly connected to the left side of the baffle (312).

2. The reaction vessel for a biological proteolytic reaction according to claim 1, characterized in that: Two support frames (4) are fixedly connected to the outer surface of the vessel body (1), and a positioning plate (5) is fixedly connected to the bottom surface of each support frame (4).

3. The reaction vessel for a biological proteolytic reaction according to claim 1, characterized in that: A feeding pipe (6) is fixedly connected to the upper part of the outer surface of the vessel body (1), and a discharge pipe (7) is fixedly connected to the lower part of the outer surface of the vessel body (1).

4. The reaction vessel for a biological proteolytic reaction according to claim 1, characterized in that: The inside of the vessel body (1) is provided with a rotating rod (8), the power output end of the rotary motor (2) passes through the vessel body (1) and is fixedly connected to the top of the rotating rod (8), and a number of stirring blades (9) are fixedly connected to the outer surface of the rotating rod (8).

5. The reaction vessel for a biological proteolytic reaction according to claim 4, characterized in that: The bottom end of the rotating rod (8) is rotatably connected to a bearing bracket (10), and the outer surface of the bearing bracket (10) is fixedly connected to the inner wall of the vessel body (1).

6. The reaction vessel for a biological proteolytic reaction according to claim 1, characterized in that: Each of the sampling tubes (301) has a reinforcing ring (11) fixedly connected to its outer surface, and the outer surface of each reinforcing ring (11) is fixedly connected to the inner wall of the vessel body (1).

7. The reaction vessel for a biological proteolytic reaction according to claim 1, characterized in that: The vessel body (1) is provided with several sealing caps (12) on its outside. Each sealing cap (12) is snapped onto the outside of the sampling cylinder (301). Each sealing cap (12) has anti-slip grooves (13) arranged at equal intervals on its outer surface.

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