Mouse spine fixing device

Abstract

The utility model belongs to the technical field of scientific research instruments, and particularly relates to a mouse spine fixing device which comprises an operating table main board, the operating table main board is used for placing a mouse, clamping mechanisms are arranged on the two sides of the operating table main board, and each clamping mechanism comprises a spine chuck. The spine chucks on the two sides clamp the two sides of the spine of a mouse through movement fit in the horizontal direction, the two spine chucks are connected through a chuck fixing plate, a microscope observation hole is further formed in the chuck fixing plate, and the operating table main plate is provided with a lifting mechanism for driving the operating table main plate to reciprocate in the vertical direction. According to the device, the success rate of a mouse spinal cord implantation operation in the two-photon microscopic imaging observation window is greatly improved, the operation time is greatly shortened, the complicated operation which can be completed by a few technicians with rich experience originally becomes easy to master, and the device is also easy to popularize.

Description

TECHNICAL FIELD

[0001] The utility model relates to scientific research instrument technical field, concretely is a mouse spinal column fixing device. BACKGROUND

[0002] Mouse spinal cord two-photon in vivo imaging technology is an important technology for neuroscientific, rehabilitation scientific and neuroimmunological research. The technology needs to open the dorsal bone plate of mouse spine, without damaging the spinal cord tissue of mouse, and needs to embed the optical transparent observation window into the surface of the dorsal bone plate of spine through a stable clamp, and make it integrated with the bone plate. The purpose of these operations is to observe the cell, blood flow, bioactive molecule release and other life activities in the spinal cord in real time through two-photon microscope at any designed observation time point. The technology has high requirements for operation, and the existing experimental scheme mainly comes from the original scheme of Cornell University in the United States (published data) and the clamp made by Neurotar Company in Denmark according to the scheme of Cornell University. However, the existing filing clamp has the problems of unstable mouse spine fixation, easy to cause damage to experimental mouse during operation, and inconvenient installation of microscope observation window assembly on the existing fixing clamp. SUMMARY

[0003] In order to overcome the above-mentioned defects existing in the prior art, the utility model provides a mouse spinal column fixing device.

[0004] A mouse spinal column fixing device, comprising an operating table main plate, the operating table main plate is used for placing mouse, both sides of the operating table main plate are provided with clamping mechanisms, the clamping mechanisms comprise spine clamping heads, both sides of the spine clamping heads clamp the both sides of mouse spine through horizontal direction movement cooperation, the two spine clamping heads are connected through clamping head fixing plates, the clamping head fixing plates are also provided with microscope observation holes, the operating table main plate is provided with a lifting mechanism for driving it to reciprocate in vertical direction.

[0005] Further, the spine clamping head is inserted on the spine clamp rod, the bottom of the spine clamp rod is connected with the lead screw through the lead screw sliding block, the lead screw sliding block is slidingly connected with the lead screw sliding table, and the lead screw sliding table is fixedly installed on the bottom plate through the base.

[0006] Further, one end of the lead screw is provided with a driving wheel, the driving wheel is connected with a driven wheel through a belt transmission, the driven wheel is coaxially arranged with and fixedly connected with a transition wheel, the transition wheel and the transmission wheel are connected through a belt transmission, and the driven wheel and the transition wheel are rotatably installed on the adjusting frame.

[0007] Further, the two transmission wheels are directly connected through a rotating shaft, the rotating shaft is rotatably installed on a fixed seat, the fixed seat is arranged on a fixed plate, and a manual handle is further arranged on the rotating shaft.

[0008] Further, the two sides of the operating table main plate are rotatably installed with operating table side plates through connecting shafts, and an anesthetic gas pipe fixing seat is installed on the upper end face of one of the operating table side plates.

[0009] Further, the lifting mechanism comprises an operating table support rod fixedly connected with the operating table main plate, the operating table support rod is fixedly installed on a lifting sliding block, and the lifting sliding block is slidingly connected with the support column.

[0010] Further, the support column is provided with a rack, the rack is in matched connection with a gear, the gear is installed in the lifting sliding block through an adjusting shaft, the two ends of the adjusting shaft extend out of the lifting sliding block and are connected with knobs, limit columns are arranged on the top and bottom of the rack, and a locking wrench is arranged on the lifting sliding block.

[0011] Further, V-shaped grooves are arranged on the opposite faces of the two spinal column clamping heads.

[0012] Further, a waist-shaped groove is arranged on the clamping head fixing plate, screw holes corresponding to the waist-shaped groove are arranged on the spinal column clamping head, the clamping head fixing plate is in U-shaped structure, and fixing holes are arranged on the side wall of the clamping head fixing plate.

[0013] Further, the support column is further provided with an adjusting scale.

[0014] Due to the above technical scheme, the beneficial technical effects of the utility model are as follows:

[0015] 1. In the utility model, the two spinal column clamping heads are simultaneously moved to the middle, the mouse's spinal column is clamped, the fixed position of the spinal column is located on the median line of the body, then the clamping head fixing plate is connected with the two spinal column clamping heads, the spinal column clamping head can be taken off from the clamping mechanism, then the clamping head is installed on the bracket of the two-photon microscope, the microscope observation hole is arranged on the clamping head fixing plate, and the staff can conveniently observe the vital signs in the animal spinal cord.

[0016] 2. In the utility model, the lifting mechanism is arranged, the operating table main plate is driven to move linearly in the vertical direction, the mouse is lifted to a suitable height, then the mouse's spinal column is fixed through the spinal column clamping head, the height of the operating table main plate is convenient to adjust, different sizes of mice can be adapted, and the applicability of the device is increased.

[0017] 3. In the utility model, when the operating table main plate is lifted to a suitable height, the staff fixes the mouse with one hand and rotates the hand-operated wheel with the other hand, the spinal column clamping heads on the two sides of the mouse are synchronously moved to the middle, and then the mouse's spinal column is clamped, the operation is convenient, and the staff can realize the clamping of the mouse's spinal column through single-handed operation of the hand-operated wheel. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 This is a schematic diagram of the structure of a mouse spinal fixation device according to the present invention. Figure 1 ;

[0019] Figure 2 This is a schematic diagram of the structure of a mouse spinal fixation device according to the present invention. Figure 2 ;

[0020] Figure 3 This is a schematic diagram showing the cooperation between the spinal clamp and the clamp fixing plate in this utility model;

[0021] Figure 4 This is a schematic diagram of the structure of the spinal clamp in this utility model;

[0022] Figure 5 This is a schematic diagram of the lifting mechanism in this utility model.

[0023] In the diagram: 1. Fixing plate; 2. Base; 3. Lead screw slide; 4. Lead screw slider; 5. Spinal clamp rod; 6. Spinal clamp; 7. Clamp fixing plate; 8. Lifting slider; 9. Support column; 10. Operating table support rod; 11. Operating table main board; 12. Operating table side plate; 13. Anesthesia gas delivery tube fixing seat; 14. Manual dial wheel; 15. Transmission wheel; 16. Transition wheel; 17. Driven wheel; 18. Drive wheel; 19. Rack; 20. Knob; 21. Screw hole; 22. Waist-shaped groove; 23. V-groove; 24. Fixing hole; 25. Microscope observation hole; 26. Limiting post; 27. Locking wrench. Detailed Implementation

[0024] To more clearly illustrate the technical solution of this utility model, the following description is made in conjunction with the accompanying drawings. Obviously, the drawings described below are only one embodiment of this utility model. For those skilled in the art, other embodiments can be obtained based on these drawings and embodiments without creative effort, and all of them fall within the protection scope of this utility model.

[0025] according to Figures 1-5 As shown, a mouse spine fixation device includes a main operating table 11 for placing a mouse. The main operating table 11 has clamping mechanisms on both sides, each clamping mechanism including a spine clamp 6. The two spine clamps 6 clamp the sides of the mouse spine by moving horizontally. The two spine clamps 6 are connected by a clamp fixing plate 7. The clamp fixing plate 7 is also provided with a microscope observation hole 25. The main operating table 11 is equipped with a lifting mechanism that drives it to reciprocate vertically.

[0026] In the above specific technical scheme, by moving the two side spine clamps 6 towards the middle at the same time, the mouse spine is clamped to ensure that the fixed position of the spine is located on the median line of the body, then the clamp fixing plate 7 is connected with the two spine clamps 6, the spine clamps 6 can be removed from the clamping mechanism, then the clamp fixing plate 7 is installed on the bracket of the two-photon microscope, the clamp fixing plate 7 is provided with a microscope observation hole 25, which is convenient for the staff to observe the vital signs in the animal spinal cord, preferably, the total volume and mass of the spine clamps 6 clamped on the mouse spine and the clamp fixing plate 7 are small, which is convenient for placing on the mouse and does not hinder the daily activities of the mouse, in actual use, the total volume of the spine clamps 6 and the clamp fixing plate 7 is designed to be 124mm 3 , and the mass is 0.99 grams; by setting the lifting mechanism, the operating table main plate 11 can be driven to move linearly in the vertical direction, and the mouse can be lifted to an appropriate height, which is convenient for fixing the mouse spine by the spine clamps 6, the height adjustment of the operating table main plate 11 is convenient, which can adapt to mice of different sizes, and the applicability of the device is increased.

[0027] As shown in Figures 1-2 , the spine clamp 6 is inserted into the spine clamp rod 5, the bottom of the spine clamp rod 5 is connected with the lead screw through the lead screw block 4, the lead screw block 4 is slidingly connected with the lead screw sliding table 3, and the lead screw sliding table 3 is fixedly installed on the base plate 1 through the base 2.

[0028] One end of the lead screw is provided with a driving wheel 18, the driving wheel 18 is connected with a driven wheel 17 through a belt transmission, the driven wheel 17 is coaxially arranged with a transition wheel 16 and is fixedly connected with the transition wheel 16, the transition wheel 16 is connected with a transmission wheel 15 through a belt transmission, and the driven wheel 17 and the transition wheel 16 are rotatably installed on the adjusting frame.

[0029] The two transmission wheels 15 are directly connected through a rotating shaft, the rotating shaft is rotatably installed on a fixed seat, the fixed seat is arranged on a fixed plate, and the rotating shaft is further provided with a manual dial 14.

[0030] In the above specific technical scheme, when the operating table main plate 11 is lifted to an appropriate height, the staff fixes the mouse with one hand and rotates the manual dial 14 with the other hand, the manual dial 14 drives the two transmission wheels 15 to rotate, the transmission wheel 15 drives the transition wheel 16 to rotate through the belt, the transition wheel 16 drives the driving wheel 18 to rotate through the driven wheel 17 and the belt, and the driving wheel 18 drives the lead screw to rotate, so that the spine clamps 6 on both sides of the mouse move towards the middle at the same time, thereby realizing the clamping of the mouse spine, which is convenient to operate, and the staff can realize the clamping of the mouse spine by operating the manual dial 14 with one hand.

[0031] As shown in Figure 1As shown, the two sides of the operating table main plate 11 are rotatably installed with operating table side plates 12 through connecting shafts, and the upper end face of one of the operating table side plates 12 is installed with an anesthesia gas pipe fixing seat.

[0032] In the above specific technical scheme, the anesthesia gas pipe is installed on the anesthesia gas pipe fixing seat, after the mouse is placed on the operating table main plate 11, the mouth of the mouse is inserted into the anesthesia gas pipe, so that the mouse is continuously anesthetized, the angle between the operating table side plate 12 and the operating table main plate 11 is adjusted, the limbs and head of the mouse are lowered, and the back is raised, so that the spine is in normal physiological curvature during the operation.

[0033] As shown in Figure 1 and Figure 5 The lifting mechanism includes an operating table support rod 10 fixedly connected with the operating table main plate 11, the operating table support rod 10 is fixedly installed on a lifting sliding block 8, and the lifting sliding block 8 is slidably connected with a support column 9.

[0034] The support column 9 is provided with a rack 19, the rack 19 is in matched connection with a gear, the gear is installed in the lifting sliding block 8 through an adjusting shaft, the two ends of the adjusting shaft extend out of the lifting sliding block 8 and are connected with a knob 20, the top and bottom of the rack 19 are each provided with a limiting column 26, and the lifting sliding block 8 is provided with a locking wrench 27.

[0035] In the above specific technical scheme, the mouse is placed at the middle position of the operating table main plate 11 and the spine is raised, and the back muscle is exposed, the spine is rotated, the knob 20 is rotated, the lifting sliding block 8 moves along the length direction of the support column 9, the lifting sliding block 8 is connected with the operating table main plate 11 through the operating table support rod 10, so that the staff member can adjust the spine of the mouse to a suitable height, and then the manual dial 14 is rotated, so that the spine clamp 6 clamps the two sides of the spine of the mouse.

[0036] Preferably, the lifting sliding block 8 is provided with the locking wrench 27, the locking wrench 27 includes a rotating handle and a threaded rod in matched connection with the lifting sliding block 8, when the lifting sliding block moves to a suitable height, the staff member rotates the rotating handle, the end of the threaded rod is tightly abutted against the support column 9, so that the height of the lifting sliding block 8 is locked.

[0037] Preferably, the lifting sliding block 8 is provided with a gear (not shown in the figure), the knob 20 drives the gear to rotate by rotating the knob 20, the lifting sliding block 8 can reciprocatingly move up and down through the matched connection between the gear and the rack 19.

[0038] As shown in Figure 4 The opposite faces of the two spine clamps 6 are provided with V-shaped grooves 23, so that the spine of the mouse can be clamped.

[0039] As shown in Figure 3As shown, the chuck fixing plate 7 is provided with a waist-shaped groove 22, the spinal column chuck 6 is provided with a screw hole 21 corresponding to the waist-shaped groove 22, the chuck fixing plate 7 is of a U-shaped structure, and the side wall of the chuck fixing plate 7 is provided with a fixing hole 24.

[0040] In the above specific technical scheme, the chuck fixing plate 7 is convenient to fix, the screw passes through the waist-shaped groove 22 and is connected with the screw hole 21, the staff can observe the mouse's spinal column through the microscope through the observation hole 25, and the fixing hole 24 on the side wall of the chuck fixing plate 7 is convenient to fix the chuck fixing plate 7 on the two-photon microscope.

[0041] The support 9 is further provided with an adjusting scale, so that the height of the lifting slide 8 can be adjusted according to the size of the mouse.

[0042] It is worth mentioning that the whole device can be subjected to high-pressure sterilization treatment.

[0043] The specific working process of the device is as follows: the staff rotates the manual dial 14 to move the spinal column chucks 6 to the maximum on both sides, places the mouse on the operating head main plate after the mouse is preliminarily anesthetized, adjusts the position of the mouse to make the required spinal column part in the middle of the operating head main plate, inserts the mouth of the mouse into the anesthetic trachea to make the mouse continuously anesthetized, adjusts the operating table side plate 12 to make the limbs and head of the mouse droop, and the back is provided, cuts the back muscle, exposes the spinal column, rotates the knob 20, adjusts the lifting slide to make the height of the spinal column of the mouse consistent with the height of the V-shaped groove 23 of the spinal column chuck 6, moves the manual dial 14 with one hand, maintains the posture of the spinal column of the mouse with one hand, makes the two spinal column chucks 6 clamp the two sides of the spinal column, then installs the chuck fixing plate 7 on the spinal column chucks 6, rotates the manual dial 14 to move the spinal column clamp rod 5 to both sides, and the spinal column chucks 6 are completely separated from the spinal column clamp rod 5, so that the fixation of the spinal column clamp on the mouse is completed. The device greatly improves the success rate of the spinal cord of the mouse in the two-photon microscopic imaging observation window implantation operation, and shortens the operation time by about 40%. The device also makes the complex operation that can only be completed by a few experienced technical personnel easy to master and easy to popularize.

[0044] The above examples are only exemplary embodiments of the device and are not used to limit the device, and the protection scope of the device is defined by the claims. Those skilled in the art can make various modifications or equivalent replacements to the device within the spirit and protection scope of the device, and the modification or equivalent replacement should also be regarded as falling within the protection scope of the device.

Claims

1. A mouse spinal column fixation device comprising a surgical table main plate (11), characterized in that, The operating table main plate (11) is used for placing mice, both sides of the operating table main plate (11) are provided with clamping mechanisms, the clamping mechanisms include spine clamping heads (6), both sides of the spine clamping heads (6) clamp both sides of the spine of the mice through horizontal direction movement cooperation, the two spine clamping heads (6) are connected through a clamping head fixing plate (7), the clamping head fixing plate (7) is further provided with a microscope observation hole (25), and the operating table main plate (11) is provided with a lifting mechanism for driving the operating table main plate (11) to reciprocate in the vertical direction.

2. The mouse spinal column fixation device of claim 1, wherein, The spine clamping head (6) is inserted on the spine clamping rod (5), the bottom of the spine clamping rod (5) is connected with a lead screw through a lead screw sliding block (4), the lead screw sliding block (4) is slidingly connected with a lead screw sliding table (3), and the lead screw sliding table (3) is fixedly installed on the bottom plate (1) through a base (2).

3. The mouse spinal column fixation device of claim 2, wherein, One end of the lead screw is provided with a driving wheel (18), the driving wheel (18) is connected with a driven wheel (17) through a belt transmission, the driven wheel (17) is coaxially arranged and fixedly connected with a transition wheel (16), the transition wheel (16) and the transmission wheel (15) are connected through a belt transmission, and the driven wheel (17) and the transition wheel (16) are rotatably installed on an adjusting frame.

4. The mouse spinal column fixation device of claim 3, wherein, The two transmission wheels (15) are directly connected through a rotating shaft, the rotating shaft is rotatably installed on a fixed seat, the fixed seat is arranged on a fixed plate, and a manual dial wheel (14) is further arranged on the rotating shaft.

5. The mouse spinal column fixation device of claim 4, wherein, Both sides of the operating table main plate (11) are rotatably provided with operating table side plates (12) through connecting shafts, and the upper end face of one of the operating table side plates (12) is provided with an anesthetic gas supply pipe fixing seat.

6. A spinal fixation device for a mouse according to claim 5, wherein, The lifting mechanism comprises an operating table support rod (10) fixedly connected with the operating table main plate (11), the operating table support rod (10) is fixedly installed on a lifting sliding block (8), and the lifting sliding block (8) is slidingly connected with a support column (9).

7. A spinal fixation device for a mouse according to claim 6, wherein The support column (9) is provided with a rack (19), the rack (19) is connected with a gear, the gear is installed in the lifting sliding block (8) through an adjusting shaft, both ends of the adjusting shaft extend out of the lifting sliding block (8) and are connected with knobs (20), the top and bottom of the rack (19) are provided with limiting columns (26), and the lifting sliding block (8) is provided with a locking wrench (27).

8. The mouse spinal column fixation device of claim 7, wherein, V-shaped grooves (23) are arranged on opposite surfaces of the two spine clamping heads (6).

9. The mouse spinal column fixation device of claim 8, wherein, A waist-shaped groove (22) is arranged on the clamping head fixing plate (7), screw holes (21) corresponding to the waist-shaped groove (22) are arranged on the spine clamping heads (6), the clamping head fixing plate (7) is in a U-shaped structure, and fixing holes (24) are arranged on the side walls of the clamping head fixing plate (7).

10. The mouse spinal column fixation device of claim 8, wherein, The support column (9) is further provided with an adjusting scale.

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