Antigen and antibody purification device with stacked elution structure

Abstract

The utility model discloses an antigen and antibody purification device with a stacked elution structure, which comprises a mounting rack, the mounting rack is of a rectangular plate structure, a heating plate is mounted on the inner layer of the mounting rack, through holes are uniformly formed in the mounting rack, the through holes in the mounting rack are mounted with positioning buckles, and the positioning buckles are mounted on the mounting rack. And the positioning buckle and the mounting frame form a frame body structure for limiting the elution and purification device. According to the antigen and antibody purification device with the stacked elution structure, a first-order elution column, a second-order elution column and a third-order elution column are stacked in the device in the vertical direction to form a group of elution pieces, and along with communication between a shunt ring and a four-way valve pipe, a gradient elution structure is constructed in the device; by arranging eluents with different concentrations or different properties in different elution column superposition units, a complex and accurate elution gradient can be constructed, the elution efficiency of target antigens and antibodies is further improved through accurate gradient elution, and the purity of purified products is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of bioengineering technology, concretely to an antigen antibody purification device with superimposed elution structure. BACKGROUND

[0002] In biomedical research and pharmaceutical industry, the purification of antigen antibody is very important, and the preparation of high specificity and high titer antibody is the basis of experimental immunology technology, and the quality of antibody will directly affect the success or failure of the test. Purified antibody is usually used for direct detection of antigen, at this time the antibody is labeled with an easily identifiable marker for binding and detecting the antigen under study.

[0003] The traditional purification elution device has many limitations. At present, the traditional elution equipment is mostly designed as a single channel structure, which requires repeated processing of the system, low overall elution efficiency, and difficulty in ensuring complete elution of antigen antibody in the adsorption medium. In addition, the traditional single channel device only uses a single substance for elution, and the purification treatment of multiple antigen antibody complexes often requires multiple runs, which not only increases the operation time, but also may cause loss of antigen antibody activity due to multiple treatments, thereby affecting the purification effect and biological activity of the target product. SUMMARY

[0004] The utility model aims at providing an antigen antibody purification device with superimposed elution structure to solve the problems of the traditional elution device mentioned in the background.

[0005] To achieve the above purpose, the utility model provides the following technical scheme: an antigen antibody purification device with superimposed elution structure, comprising a mounting bracket, which is set as a rectangular plate structure, a heating plate is installed in the inner layer of the mounting bracket, and through holes are uniformly provided in the mounting bracket, and the through holes in the mounting bracket are installed with positioning buckles, and the positioning buckles and the mounting bracket constitute a bracket structure for limiting the elution and purification device;

[0006] The positioning buckles are arranged in a vertical direction, and two positioning buckles form a group, and there are three groups in total, and each group of positioning buckles is installed with a first elution column, a second elution column and a third elution column from top to bottom, respectively, the first elution column is at the topmost, and a feed hopper is installed on the upper end of the first elution column;

[0007] The bottom end of the first elution column is connected to the upper end of a flow dividing ring one, and the bottom end of the flow dividing ring one is communicated with the top end of the second elution column, the bottom end of the second elution column is connected to the upper end of a flow dividing ring two, and the bottom end of the flow dividing ring two is communicated with the top end of the third elution column, and the bottom end of the third elution column is connected with a drainage pipe one;

[0008] The shunt ring one is provided with one pipeline opening on one side, and the pipeline opening of the shunt ring one is communicated with one side opening of the four-way valve pipe one, the bottom end opening of the four-way valve pipe one is connected with a drainage pipe two, the drainage pipe two is connected with the upper end opening of the four-way valve pipe two, and the bottom end opening of the four-way valve pipe two is connected with a pipeline valve, and the side opening of the four-way valve pipe two is communicated with the shunt ring two.

[0009] By adopting the technical scheme, the superimposed elution structure allows the elution effluent to act on the medium adsorbed with antigen and antibody from multiple directions at the same time.

[0010] Preferably, the positioning buckle is provided in a Y-shaped frame structure, and the positioning buckle is fixed by a threaded connection nut at one end of the mounting frame.

[0011] By adopting the technical scheme, the positioning buckle is fixed to the elution column and is stably mounted to the mounting frame.

[0012] Preferably, the first-order elution column, the second-order elution column and the third-order elution column constitute one group of elution members, and three groups of elution members are mounted in the mounting frame.

[0013] By adopting the technical scheme, the first-order elution column, the second-order elution column and the third-order elution column are in a superimposed structure, and the connection and cooperation between multiple groups of superimposed structures increase the concentration or the property of the elution liquid to construct a complex and accurate elution gradient.

[0014] Preferably, one valve is mounted in the upper end connection and the lower end connection of the shunt ring one respectively, and one blocking block is mounted in the side pipeline opening of the shunt ring one.

[0015] By adopting the technical scheme, the port not connected to the shunt ring one is sealed by the blocking block, so as to avoid the influence of external bacteria.

[0016] Preferably, one valve is mounted in the upper end connection and the lower end connection of the shunt ring two respectively, and one blocking block is mounted in the side pipeline opening of the shunt ring two.

[0017] By adopting the technical scheme, the port connected to the shunt ring two is sealed by the blocking block, so as to avoid the influence of external bacteria.

[0018] Preferably, the four-way valve pipe one is arranged above the four-way valve pipe two, one valve is arranged at each opening of the four-way valve pipe one, and the four-way valve pipe one and the four-way valve pipe two are consistent in structure.

[0019] By adopting the technical scheme, the multiple openings are arranged, so as to facilitate the mutual shunting and converging between different elution column superposition units.

[0020] Preferably, the pipeline valve is composed of a pipeline and a valve, and the valve of the pipeline valve is arranged at the bottom end pipeline.

[0021] The technical scheme makes the pipeline valve provide an additional outlet for the split sampling elution effluent.

[0022] Compared with the prior art, the antigen-antibody purification device with the superimposed elution structure has the beneficial effects that:

[0023] 1. The first-order elution column, the second-order elution column and the third-order elution column are vertically superimposed to form a group of elution members in the device, and the split ring is connected with the four-way valve pipe, so that a gradient elution structure is formed in the device, different concentrations or different properties of elution liquid are arranged in different elution column superposition units, a complex and accurate elution gradient can be formed, the elution efficiency of the target antigen-antibody is further improved through accurate gradient elution, impurities can be better separated, and the purity of the product after purification is improved.

[0024] 2. The split ring and the four-way valve pipe are arranged to connect the different elution column superposition units, the valves in the upper and lower split rings and the four openings in the four-way valve pipe are controlled, so that the split and flow directions of the elution effluent are adjusted, a plurality of different elution functions are integrated in the same device, which is very beneficial to the purification of a complex antigen-antibody mixture, the loss and pollution risk of the sample caused by transfer between different devices are reduced through multifunctional integration, the overall purification time is shortened, and the work efficiency is improved. BRIEF DESCRIPTION OF DRAWINGS

[0025] Fig. 1 It is an overall splicing and assembling external three-dimensional structure schematic view of the utility model;

[0026] Fig. 2 It is an installation three-dimensional structure schematic view of a single group of first-order elution column, second-order elution column, third-order elution column and mounting frame of the utility model;

[0027] Fig. 3 It is a superimposed installation three-dimensional structure schematic view of the first-order elution column, the second-order elution column and the third-order elution column of the utility model;

[0028] Fig. 4 It is a split state three-dimensional structure schematic view of the first-order elution column, the second-order elution column and the third-order elution column of the utility model;

[0029] Fig. 5 It is an installation three-dimensional structure schematic view of the split ring one, the split ring two, the four-way valve pipe one and the four-way valve pipe two of the utility model;

[0030] Fig. 6 It is an installation three-dimensional structure schematic view of the split ring one and the four-way valve pipe one and an internal horizontal sectional three-dimensional structure schematic view of the split ring one.

[0031] In the figure: 1, mounting frame; 2, heating plate; 3, positioning buckle; 4, first-stage elution column; 5, second-stage elution column; 6, third-stage elution column; 7, feeding hopper; 8, shunt ring one; 9, shunt ring two; 10, flow guide pipe one; 11, four-way valve pipe one; 12, flow guide pipe two; 13, four-way valve pipe two; 14, pipeline valve; 15, blocking block. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0033] Please refer to Figs. 1-6 The utility model provides a kind of technical scheme: a kind of antigen antibody purification device with superimposed elution structure, including mounting frame 1, heating plate 2, positioning buckle 3, first-stage elution column 4, second-stage elution column 5, third-stage elution column 6, feeding hopper 7, shunt ring one 8, shunt ring two 9, flow guide pipe one 10, four-way valve pipe one 11, flow guide pipe two 12, four-way valve pipe two 13, pipeline valve 14 and blocking block 15;

[0034] Among them, mounting frame 1 is set to rectangular plate body structure, heating plate 2 is installed in mounting frame 1 inner layer, and the through hole is evenly provided in mounting frame 1 inner, and the through hole in mounting frame 1 inner is installed with positioning buckle 3, positioning buckle 3 and mounting frame 1 constitute the frame structure of limiting elution purification device, positioning buckle 3 is set to Y-shaped frame structure, and the end of positioning buckle 3 through mounting frame 1 is fixed using threaded connection nut;

[0035] Positioning buckle 3 is vertically arranged, and 2 positioning buckles 3 are 1 group, and 3 groups are set, and each group of positioning buckles 3 is installed with first-stage elution column 4, second-stage elution column 5 and third-stage elution column 6 from top to bottom respectively, first-stage elution column 4 is in the uppermost, and feeding hopper 7 is installed on the upper end of first-stage elution column 4;

[0036] The bottom end of first-stage elution column 4 is connected to the upper end of shunt ring one 8, the bottom end of shunt ring one 8 is communicated with the top end of second-stage elution column 5, the bottom end of second-stage elution column 5 is connected to the upper end of shunt ring two 9, the bottom end of shunt ring two 9 is communicated with the top end of third-stage elution column 6, and the bottom end of third-stage elution column 6 is connected with flow guide pipe one 10, first-stage elution column 4, second-stage elution column 5 and third-stage elution column 6 constitute 1 group of elution pieces, and 3 groups of elution pieces are installed in mounting frame 1;

[0037] The Figs. 1-6As shown, the first elution column 4 is filled with affinity chromatography medium, and protein A, protein G, etc. are used for antibody purification. The second elution column 5 is filled with ion exchange chromatography medium, and anion or cation exchange resin is used to separate antigen-antibody according to charge. The third elution column 6 is filled with gel filtration chromatography medium, so that antigen-antibody is separated according to molecular size to meet the purification needs of different antigen-antibody. The funnel structure at both ends of the first elution column 4, the second elution column 5 and the third elution column 6 is used to connect the shunt ring I 8 and the shunt ring II 9, as shown in Figs. 1-2 As shown, the positioning buckle 3 is installed outside the first elution column 4, the second elution column 5 and the third elution column 6 and is connected to the mounting rack 1. The mounting rack 1 is made of high-strength metal to provide support. The heating plate 2 provided in the mounting rack 1 further provides a heating environment according to the elution needs. The high-temperature environment assists the further processing of the elution in the first elution column 4, the second elution column 5 and the third elution column 6. Through the stacked structure, efficient use of space is provided for the vertical space layout inside the mounting rack 1. At the same time, the corresponding filler in multiple elution groups can be replaced, so that different elution columns have different elution effects. Through the linkage and cooperation between the elution columns, more accurate purification effect can be provided for complex antigen-antibody;

[0038] The shunt ring I 8 has one pipeline opening on each side, and the pipeline opening of the shunt ring I 8 is in communication with one side opening of the four-way valve pipe I 11. The bottom end opening of the four-way valve pipe I 11 is connected with the drainage pipe II 12, and the drainage pipe II 12 is connected with the upper end opening of the four-way valve pipe II 13. The bottom end opening of the four-way valve pipe II 13 is connected with the pipeline valve 14, and the side opening of the four-way valve pipe II 13 is in communication with the shunt ring II 9. One valve is installed in the upper end connection and the lower end connection of the shunt ring I 8, and one sealing block 15 is installed in the side pipeline opening of the shunt ring I 8. One valve is installed in the upper end connection and the lower end connection of the shunt ring II 9, and one sealing block 15 is installed in the side pipeline opening of the shunt ring II 9. The four-way valve pipe I 11 is arranged above the four-way valve pipe II 13, and one valve is arranged at each opening of the four-way valve pipe I 11. The four-way valve pipe I 11 and the four-way valve pipe II 13 are consistent in structure. The pipeline valve 14 is composed of a pipeline and a valve, and the valve of the pipeline valve 14 is at the bottom end pipeline.

[0039] The specific embodiments of the application are described in detail in combination with the drawings of the specification Figs. 1-6 As shown, the shunt ring I 8 is installed between the first elution column 4 and the second elution column 5. The shunt ring I 8 is in communication with the four-way valve pipe I 11 through the side opening. The four-way valve pipe I 11 is in communication with another group of shunt ring I 8 beside it, so that the elution effluent of two groups of first elution column 4 is exchanged or exchanged to the second elution column 5. The elution effluent of the second elution column 5 is communicated with the four-way valve pipe II 13 through the shunt ring II 9, and is communicated with one group of third elution column 6 beside it, as shown in Fig. 1As shown, the confluence of the elution effluent of the four-way valve pipe one 11 and the four-way valve pipe two 13 is guided through the drainage pipe two 12, and the valves arranged inside the shunt ring one 8 and the shunt ring two 9 cooperate with the four-way valve pipe one 11 and the four-way valve pipe two 13 to realize the shunting and confluence of the elution effluent, thereby realizing the multi-directional elution effluent flow and gradient elution effect, and the flow control is realized by controlling the opening size of all the valves in the device, the elution effluent from the upper elution column can be distributed to the lower elution column according to the preset proportion or directly led out for collection, compared with the traditional single-direction elution purification device, the antigen or antibody can be more fully eluted from the medium.

[0040] Working principle: when the antigen antibody purification device with the superimposed elution structure is used, the first-order elution column 4, the second-order elution column 5 and the third-order elution column 6 are stacked and installed, different types of adsorption media are filled in each elution column, so that the antigen antibody is easily eluted from top to bottom step by step, further adapting to the purification requirements of different antigens and antibodies, when the elution effluent of the first-order elution column 4 flows to the second-order elution column 5, it passes through the shunt ring one 8, when the elution effluent of the second-order elution column 5 flows to the third-order elution column 6, it passes through the shunt ring two 9, when the shunt ring one 8 and the shunt ring two 9 perform shunting operation according to the elution requirement, the liquid flow direction is adjusted by adjusting the four valve structures of the four-way valve pipe one 11 and the four-way valve pipe two 13, thereby realizing the purification of complex antigen antibody mixture, multi-functional integration, reducing the loss and pollution risk caused by sample transfer between different devices, at the same time, the overall purification time is shortened, the work efficiency is improved, and the overall practicability is increased.

[0041] Although the embodiments of the present application have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the present application, the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. An antigen antibody purification device provided with a superimposed elution structure, comprising: a mounting frame (1) provided as a rectangular plate structure, a heating plate (2) is mounted in the inner layer of the mounting frame (1), and through holes are uniformly provided in the mounting frame (1), and the through holes in the mounting frame (1) are mounted with positioning buckles (3), and the positioning buckles (3) and the mounting frame (1) constitute a frame structure for limiting the elution purification device; characterized in that the positioning buckles (3) are arranged in a vertical direction, and two positioning buckles (3) form a group, and there are three groups in total, and each group of positioning buckles (3) is mounted with a first-stage elution column (4), a second-stage elution column (5) and a third-stage elution column (6) from top to bottom, respectively, the first-stage elution column (4) is at the uppermost position, and a feed hopper (7) is mounted at the upper end of the first-stage elution column (4); the bottom end of the first-stage elution column (4) is connected to the upper end of a flow dividing ring I (8), the bottom end of the flow dividing ring I (8) is in communication with the top end of the second-stage elution column (5), the bottom end of the second-stage elution column (5) is connected to the upper end of a flow dividing ring II (9), the bottom end of the flow dividing ring II (9) is in communication with the top end of the third-stage elution column (6), and the bottom end of the third-stage elution column (6) is connected with a flow guide pipe I (10); one pipeline opening is provided on each side of the flow dividing ring I (8), and the pipeline opening of the flow dividing ring I (8) is in communication with one side opening of a four-way valve pipe I (11), the bottom end opening of the four-way valve pipe I (11) is connected with a flow guide pipe II (12), the flow guide pipe II (12) is connected with the upper end opening of a four-way valve pipe II (13), the bottom end opening of the four-way valve pipe II (13) is connected with a pipeline valve (14), and the side opening of the four-way valve pipe II (13) is in communication with the flow dividing ring II (9).

2. An antigen-antibody purification device provided with a superposed elution structure according to claim 1, characterized in that: The positioning buckles (3) are arranged as a Y-shaped frame structure, and the end of the positioning buckles (3) penetrating the mounting frame (1) is fixed by a threaded connection nut.

3. An antigen-antibody purification device provided with a superposed elution structure according to claim 1, characterized in that: The first-stage elution column (4), the second-stage elution column (5) and the third-stage elution column (6) constitute a group of elution components, and three groups of elution components are mounted in the mounting frame (1).

4. The antigen-antibody purification device with superposed elution structure according to claim 1, characterized in that: One valve is mounted in each of the upper end connection and the lower end connection of the flow dividing ring I (8), and one blocking block (15) is mounted with the side pipeline opening of the flow dividing ring I (8).

5. The antigen-antibody purification device with superposed elution structure according to claim 1, characterized in that: One valve is mounted in each of the upper end connection and the lower end connection of the flow dividing ring II (9), and one blocking block (15) is mounted with the side pipeline opening of the flow dividing ring II (9).

6. An antigen-antibody purification device provided with a superposed elution structure according to claim 1, characterized in that: The four-way valve pipe I (11) is arranged above the four-way valve pipe II (13), one valve is arranged at each of the four openings of the four-way valve pipe I (11), and the four-way valve pipe I (11) and the four-way valve pipe II (13) are identical in structure.

7. An antigen-antibody purification device provided with a superposed elution structure according to claim 1, characterized in that: The pipeline valve (14) is composed of a pipeline and a valve, and the valve of the pipeline valve (14) is at the bottom end of the pipeline.

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