Multiplex PCR (Polymerase Chain Reaction) detection kit

Abstract

The utility model relates to a multiplex PCR (Polymerase Chain Reaction) detection kit, which comprises a kit, a reagent groove arranged on the inner side of the kit and a kit cover hinged on the outer side of the kit, a connecting part is arranged on the inner side of the kit cover, a rubber layer is fixed on the outer side of the connecting part in a buckling way, a limiting part is fixedly connected on the outer side of the rubber layer, and the limiting part is arranged in a net shape. According to the kit, the elastic layer is matched with the linear grooves and the expansion grooves, so that when the kit cover is closed to seal the kit, the elastic layer is split into a plurality of small blocks which are arranged in a rectangular shape under the opening of the linear grooves and the expansion grooves to wrap reagents placed in the kit, the limiting and reinforcing effects are achieved, and the reagents are effectively prevented from shaking in the kit; and the stability of the kit for storing and placing the reagents is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to reagent box technical field, concretely relates to a multiplex PCR detection reagent box. BACKGROUND

[0002] ‌Multiplex PCR detection is a molecular biology detection method based on polymerase chain reaction technology, can amplify multiple nucleic acid fragments in the same reaction system, its basic principle is same with conventional PCR, the difference lies in that more than one pair of primers is added in multiplex PCR reaction system, each pair of primaments is combined at the corresponding site of template respectively, and finally multiple DNA fragments of purpose are amplified‌.

[0003] When the existing reagent box is used to store reagents and move, the reagents stored in the box are easily affected by inertia and gravity, and the phenomenon of lifting up and falling down occurs, so that the liquid in the reagents shakes, and the subsequent normal use of the sample liquid in the reagents is affected. UTILITY MODEL CONTENT

[0004] Based on the above description, the utility model provides a multiplex PCR detection reagent box to solve the problem that the reagents stored in the box are easily affected by inertia and gravity when the existing reagent box is used to store reagents and move, and the liquid in the reagents shakes.

[0005] The technical scheme for solving the above technical problem is as follows: a multiplex PCR detection reagent box, comprising a reagent box, a reagent groove arranged on the inner side of the reagent box, and a box cover hingedly arranged on the outer side of the reagent box, wherein the inner side of the box cover is provided with a connecting part, the outer side of the connecting part is fixed with a rubber layer in a buckling manner, the outer side of the rubber layer is fixedly connected with a limiting part, and the limiting part is arranged in a mesh shape.

[0006] Based on the above technical scheme, the utility model can also be improved as follows.

[0007] Further, the rubber layer is inserted into the inner side of the box cover, a mounting groove is arranged on the side of the rubber layer away from the reagent box, the mounting groove is arranged in a rectangular shape, and a tapered groove penetrating through the rubber layer is arranged at one end of the mounting groove close to the reagent box.

[0008] Further, the connecting part comprises a cylindrical body and a spherical end, the cylindrical body is fixedly connected to the inner wall of the box cover on the side away from the reagent box and is inserted into the inner side of the mounting groove, and the spherical end is integrally formed at one end of the cylindrical body close to the reagent box.

[0009] Further, the surface of the spherical end is attached to the inner wall of the tapered groove, and the diameter of the spherical end is greater than the diameter of the mounting groove.

[0010] Furthermore, the limiting part includes an elastic layer, a linear groove, and an expansion groove. The elastic layer is fixedly connected to the side of the rubber layer near the reagent kit, and the edge of the elastic layer near the reagent kit is inclined towards the center.

[0011] Furthermore, the linear grooves are formed on the side of the elastic layer near the reagent kit, and the linear grooves are distributed in a mesh pattern.

[0012] Furthermore, the expansion groove is formed on the side of the elastic layer near the reagent kit and is located at the intersection of the linear grooves.

[0013] Compared with the prior art, the technical solution of this application has the following beneficial technical effects:

[0014] 1. This utility model uses an elastic layer, in conjunction with linear grooves and expansion grooves, to seal the reagent kit when the lid is closed. The elastic layer, under the opening of the linear grooves and expansion grooves, splits into multiple small rectangular blocks to wrap the reagents placed inside the reagent kit, playing a role in limiting and reinforcing, effectively preventing the reagents from shaking inside the reagent kit, and increasing the stability of the reagent kit for reagent storage and placement.

[0015] 2. This utility model uses a connecting part and a rubber layer that is fastened to the outside of the connecting part for fixation. The fastening method simplifies the installation and disassembly of the elastic layer, making it easy to install and replace. During use, the elastic layer can be replaced at any time to maintain its elastic effect and ensure the stability of the reagent limiting reinforcement in the reagent kit. Attached Figure Description

[0016] Figure 1 A schematic diagram of the structure of a multiplex PCR detection kit provided in an embodiment of this utility model;

[0017] Figure 2 This is an exploded view of the box lid and rubber layer in an embodiment of this utility model;

[0018] Figure 3 This is an exploded structural diagram of the rubber layer and the limiting part in an embodiment of this utility model;

[0019] Figure 4 This is a cross-sectional structural diagram showing the connection relationship between the connecting part and the rubber layer in an embodiment of this utility model;

[0020] The attached diagram lists the components represented by each number as follows:

[0021] 1. Reagent kit; 11. Reagent reservoir; 2. Lid; 3. Rubber layer; 4. Mounting slot; 41. Conical groove; 5. Connecting part; 51. Column; 52. Spherical end; 6. Limiting part; 61. Elastic layer; 62. Linear groove; 63. Expansion groove. DETAILED DESCRIPTION

[0022] For the purpose of facilitating the understanding of the present application, the present application will be described more fully below with reference to the accompanying drawings. In the drawings, embodiments of the present application are shown. However, the present application can be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete. It is noted that the use of any and all examples, or exemplary language (e.g., "the best", "at least the best", etc.), is intended to better illuminate the application, and is not a limitation on the scope of the application unless otherwise indicated.

[0023] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used in the description of the application herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.

[0024] Referring to Figures 1-4 The utility model discloses a multiple PCR detection kit, including reagent box 1, the reagent groove 11 of setting reagent box 1 inside and the hinge in reagent box 1 outside box cover 2, the inside of box cover 2 is provided with connecting portion 5, the outside of connecting portion 5 is fixed with rubber layer 3 through the mode of buckling, the outside of rubber layer 3 is fixedly connected with limiting portion 6, and limiting portion 6 is arranged in the net shape.

[0025] Referring to Figure 4 Rubber layer 3 is inserted into the inside of box cover 2, and a mounting groove 4 is formed in the side of rubber layer 3 away from reagent box 1, the mounting groove 4 is distributed in a rectangular shape, and a tapered groove 41 is formed in the end of mounting groove 4 close to reagent box 1 and penetrates rubber layer 3, the tapered groove 41 provides space for accommodating spherical end 52, so that connecting portion 5 and rubber layer 3 are buckled and fixed, and the elastic layer 61 is not affected.

[0026] Referring to Figure 4 Connecting portion 5 includes a cylindrical body 51 and a spherical end 52, the cylindrical body 51 is fixedly connected to the inside wall of box cover 2 away from reagent box 1 and is inserted into the inside of mounting groove 4, the spherical end 52 is integrally formed at the end of cylindrical body 51 close to reagent box 1, the surface of spherical end 52 is attached to the inner wall of tapered groove 41, and the diameter of spherical end 52 is greater than the diameter of mounting groove 4, under the action of the deformation capacity of rubber layer 3, rubber layer 3 can expand through the deformation of surface mounting groove 4, and is buckled outside the cylindrical body 51 through spherical end 52, after buckling, the mounting groove 4 on the surface of rubber layer 3 is deformed and restored and is fixed between spherical end 52 and box cover 2, when rubber layer 3 is disassembled, it is only necessary to pull rubber layer 3 to make mounting groove 4 expand again and move out of the outside of cylindrical body 51 and spherical end 52, the mounting and dismounting of the whole rubber layer 3 is simple and convenient, and the elastic layer 61 on the outside is also replaced synchronously under the influence of the dismounting and mounting operation of rubber layer 3, the elastic effect of elastic layer 61 is maintained for a long time, and the stability of the reagent limiting and reinforcing in reagent box 1 is ensured.

[0027] Please refer to Figure 1 The limiting part 6 comprises an elastic layer 61, linear grooves 62 and expansion grooves 63. The elastic layer 61 is fixedly connected to the rubber layer 3 on the side close to the reagent box 1. The edge of the elastic layer 61 on the side close to the reagent box 1 is inclined towards the center. The linear grooves 62 are arranged on the side of the elastic layer 61 close to the reagent box 1. The linear grooves 62 are distributed in a net shape. The expansion grooves 63 are arranged on the side of the elastic layer 61 close to the reagent box 1 and are located at the intersections of the linear grooves 62. The elastic layer 61 is a sponge layer. The elastic layer 61 is split into multiple small blocks arranged in a rectangular shape due to the arrangement of the linear grooves 62 and the expansion grooves 63. When the box cover 2 is closed to seal the reagent box 1, the elastic layer 61 can wrap the reagents placed in the reagent box 1 through the multiple small blocks and fill the gaps between the reagents, thereby playing a role of limiting and reinforcing. The phenomenon of the reagents received in the reagent box 1 being lifted up or falling down due to the effects of inertia and gravity is effectively prevented.

[0028] In this case, the singular forms "a," "an," and "the" include plural references unless the context clearly dictates otherwise. It is also to be understood that the term "or" as used herein encompasses both exclusive and inclusive meanings unless the context clearly indicates otherwise.

[0029] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A multiplex PCR detection kit, comprising a kit (1), a reagent groove (11) arranged inside the kit (1) and a cover (2) hinged outside the kit (1), characterized in that: The inner side of the box cover (2) is provided with a connecting part (5), the outer side of the connecting part (5) is fixed with a rubber layer (3) in a buckling manner, the outer side of the rubber layer (3) is fixedly connected with a limiting part (6), and the limiting part (6) is provided in a mesh shape.

2. The multiplex PCR detection kit according to claim 1, characterized in that: The rubber layer (3) is inserted into the inner side of the box cover (2), the side, away from the kit (1), of the rubber layer (3) is provided with a mounting groove (4), the mounting groove (4) is distributed in a rectangular shape, and one end, close to the kit (1), of the mounting groove (4) is provided with a tapered groove (41) penetrating through the rubber layer (3).

3. The multiplex PCR detection kit according to claim 2, characterized in that: The connecting part (5) comprises a column body (51) and a spherical end (52), the column body (51) is fixedly connected to the inner wall of the box cover (2) on the side, away from the kit (1), and is inserted into the inner side of the mounting groove (4), and the spherical end (52) is integrally formed on one end, close to the kit (1), of the column body (51).

4. The multiplex PCR detection kit according to claim 3, characterized in that: The surface of the spherical end (52) is attached to the inner wall of the tapered groove (41), and the diameter of the spherical end (52) is greater than that of the mounting groove (4).

5. The multiplex PCR detection kit according to claim 3, characterized in that: The limiting part (6) comprises an elastic layer (61), a linear groove (62) and an expansion groove (63), the elastic layer (61) is fixedly connected to the side, close to the kit (1), of the rubber layer (3), and the edge of the side, close to the kit (1), of the elastic layer (61) is inclined towards the center.

6. The multiplex PCR detection kit according to claim 5, characterized in that: The linear groove (62) is arranged on the side, close to the kit (1), of the elastic layer (61), and the linear groove (62) is distributed in a mesh shape.

7. The multiplex PCR detection kit according to claim 5, characterized in that: The expansion groove (63) is arranged on the side, close to the kit (1), of the elastic layer (61) and is located at the intersection of the linear groove (62).

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