Cloning device for induced pluripotent stem cells

Abstract

The utility model discloses an induced pluripotent stem cell clone picking device which comprises a picking device and a collecting device, the picking device comprises a supporting rod, a connecting piece and a picking ring, and the collecting device comprises a grab handle and a scraping plate. According to the utility model, the picking device and the collecting device are integrally arranged, collection can be synchronously carried out after scribing, and the two sets of devices are matched for use, so that a clone picking function with higher cleanliness and less differentiation possibility is realized, a single clone group can be picked more efficiently and more accurately, the pollution probability is reduced, and a purer clone group is obtained; the differentiation phenomenon in the subsequent subculture process is effectively avoided, so that the subculture efficiency of the induced pluripotent stem cells is higher, and an induced pluripotent stem cell product with higher quality is obtained.

Description

TECHNICAL FIELD

[0001] The utility model relates to pick clone technical field, concretely relates to a kind of induced pluripotent stem cell pick clone device. BACKGROUND

[0002] Pick clone is an important step in molecular biology experiment, usually used to select single clone from culture dish and transfer it to new container, so as to carry out further experiment and analysis.The process of pick clone involves using specific tools or equipment to select single clone from culture dish, and transfer it to well plate or other container.

[0003] When selecting, single clone group selected needs to be transferred to new culture dish or culture well using pick clone tool.Common pick clone tools are elongated metal needle, disposable cell selection needle, as multiple scribing pick is needed, thus single clone group is easily damaged, and the possibility of being contaminated is higher, so that differentiation phenomenon is easily appeared in subsequent subculture process. UTILITY MODEL CONTENT

[0004] The utility model aims at: to solve above-mentioned problem, the utility model provides a kind of induced pluripotent stem cell pick clone device.

[0005] The utility model to realize above-mentioned purpose specifically adopts following technical scheme, comprising: pick device and collection device, the pick device is set on the collection device;

[0006] The pick device includes telescopic link, connecting piece and pick ring, and the pick device is used to scribe the clone group to be picked;

[0007] The collection device includes handle and scraper, and the collection device is used to collect the clone group after processing.

[0008] As further description of the above technical solution, the connecting piece is connected with the telescopic link and the pick ring at both ends, and the connecting piece is connected with the telescopic link at one end in the shape of a circular arc.

[0009] As further description of the above technical solution, the telescopic link is arranged in the handle, and the telescopic link is connected with the switch at the end of the handle.

[0010] As further description of the above technical solution, the pick ring includes support rod and scribing needle, and the support rod is connected with the connecting piece at one end.

[0011] As further description of the above technical solution, the scribing needle is installed on the support rod.

[0012] As further description of the above technical solution, the support rod and the scribing needle are perpendicular to each other.

[0013] As a further description of the above technical solution, the scribe needle is equidistantly provided with 3-6 groups.

[0014] As a further description of the above technical solution, the handle is connected with the scraper at one end.

[0015] As a further description of the above technical solution, the scraper is provided with a hollow cavity in the middle.

[0016] As a further description of the above technical solution, the hollow cavity is symmetrically provided with a stirring device.

[0017] The beneficial effects of the present application are as follows:

[0018] The present application integrates the picking device and the collecting device, and two sets of picking and collecting can be simultaneously performed after scribing. The two devices are used in cooperation, realize the picking function with higher cleanliness and less differentiation possibility, can more efficiently and accurately pick single clone groups, reduce the pollution probability to obtain purer clone groups, effectively avoid the differentiation phenomenon in the subsequent subculture process, make the efficiency of induced pluripotent stem cell subculture higher, and harvest higher-quality induced pluripotent stem cell products.

[0019] To make the structure features and effects of the present application clearer, the present application will be described in detail below in combination with the drawings and specific embodiments. BRIEF DESCRIPTION OF DRAWINGS

[0020] Fig. 1 is a structural schematic view of the induced pluripotent stem cell picking device of the present application;

[0021] Fig. 2 is a top view of the induced pluripotent stem cell picking device of the present application.

[0022] Reference signs:

[0023] 1, picking device; 11, telescopic rod; 12, connecting piece; 13, picking ring; 131, supporting rod; 132, scribe needle; 2, collecting device; 21, handle; 211, switch; 22, scraper; 221, hollow cavity; 222, stirring device. DETAILED DESCRIPTION

[0024] To make the structure features and effects of the present application clearer, the present application will be described in detail below in combination with the drawings and specific embodiments.

[0025] As Figs. 1-2As shown in the drawings, in one embodiment, a device for picking induced pluripotent stem cell clones comprises a picking device 1 and a collecting device 2, the picking device 1 is arranged on the collecting device 2, the picking device 1 is used for scribing the clone to be picked, and the collecting device 2 is used for collecting the scribed clone.

[0026] As shown in the drawings, Figs. 1-2 As shown in the drawings, in this embodiment, the picking device 1 comprises a telescopic rod 11, a connecting piece 12 and a picking ring 13. The connecting piece 12 is located between the telescopic rod 11 and the picking ring 13, and serves to connect and adjust the angle, ensuring the stability and convenience of the device; and the connecting piece 12 connected to one end of the telescopic rod 11 is arc-shaped, which can provide a larger range of angle adjustment, allowing the user to easily adjust the angle under different operating environments and space conditions, so as to better adapt to clones of different sizes and shapes.

[0027] Further, the telescopic rod 11 is arranged inside the handle 21, and the switch 211 at the end of the telescopic rod 11 and the handle 21 is connected (similar to the pressing type telescopic mechanism composed of a sleeve, a spring, a pressing rod and a ejector rod in a ballpoint pen, which can realize telescopic function through simple pressing action, ensuring high efficiency and convenience in the operation process, which is not described in detail as prior art). Specifically, one end of the supporting rod 131 is connected to the connecting piece 12, and the other end supports the scribing needle 132, the supporting rod 131 and the scribing needle 132 are perpendicular to each other, and the scribing needle 132 is arranged in groups of 3-6 at equal intervals, thereby forming a picking ring 13 structure like a harrow, the distance and number of scribing needles 132 in each group can be adjusted according to experimental requirements, ensuring the stability and precision of the picking ring 13, and reducing the scribing frequency and damage to the clone.

[0028] As shown in the drawings, Figs. 1-2 As shown in the drawings, in this embodiment, the collecting device 2 comprises a handle 21 and a scraper 22, one end of the handle 21 is connected to the scraper 22, the scraper 22 is provided with a hollow cavity 221 in the middle, and a miniature automatic stirring device 222 is symmetrically arranged on the hollow cavity 221, which can not only accommodate the clone, but also automatically stir the clone in the scraper 22 through the built-in miniature automatic stirring device 222 (for example, by driving stirring blades or other stirring elements with a small motor, which can automatically stir the clone in the scraper 22, avoiding sedimentation or stratification), achieving uniform stirring and mixing.

[0029] Working principle: after determining the selected single cell clone group, press the switch 211 at one end of the handle 21, so that the telescopic rod 11 drives the connecting piece 12 to stretch out with the picking ring 13, then the cross-crossing method is used, that is, first gently draw a line in the horizontal direction, and then draw a line in the vertical direction once, this method can more efficiently and more aseptically obtain high-quality single ips cell colony, after the drawing is completed, press the switch 211 at one end of the handle 21, so that the telescopic rod 11 drives the connecting piece 12 to retract with the picking ring 13, then turn over the scraper 22 to gently collect the clone group obtained by the drawing process, start the micro automatic stirring device 222 on the scraper 22 to mix gently, and then subculture to a new well plate.

[0030] Through the above technical scheme, the integrated picking and collecting device 2 can effectively reduce the number of times of drawing lines, reduce the damage to the clone group, and avoid the pollution of the clone group; at the same time, the single clone group can be picked more efficiently and accurately to obtain a purer clone group, so that the differentiation phenomenon in the subsequent subculture process is effectively avoided, the subculture efficiency of induced pluripotent stem cells is higher, and the induced pluripotent stem cell product with higher quality is harvested.

[0031] The above description of the disclosed embodiments enables a person skilled in the art to implement or use the present application. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to these embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A device for selecting clones of induced pluripotent stem cells, characterized in that, include: A picking device (1) and a collecting device (2), wherein the picking device (1) is disposed on the collecting device (2); The picking device (1) includes a telescopic rod (11), a connector (12) and a picking ring (13), and the picking device (1) is used to mark the clones to be picked; The collection device (2) includes a handle (21) and a scraper (22) for collecting processed clones.

2. The induced pluripotent stem cell clone selection device according to claim 1, characterized in that, The connector (12) is connected to the telescopic rod (11) and the picking ring (13) at both ends. The shape of the end of the connector (12) connected to the telescopic rod (11) is arc-shaped.

3. The induced pluripotent stem cell clone selection device according to claim 1, characterized in that, The telescopic rod (11) is located inside the handle (21), and the telescopic rod (11) is connected to the switch (211) at the end of the handle (21).

4. The induced pluripotent stem cell clone selection device according to claim 1, characterized in that, The picking ring (13) includes a support rod (131) and a marking needle (132), and the support rod (131) is connected to the connector (12) at one end.

5. The induced pluripotent stem cell clone selection device according to claim 4, characterized in that, The marking needle (132) is mounted on the support rod (131).

6. The induced pluripotent stem cell clone selection device according to claim 4, characterized in that, The support rod (131) and the marking needle (132) are perpendicular to each other.

7. The induced pluripotent stem cell clone selection device according to claim 4, characterized in that, The marking needles (132) are arranged in 3-6 groups at equal intervals.

8. The induced pluripotent stem cell clone selection device according to claim 1, characterized in that, One end of the handle (21) is connected to the scraper (22).

9. The induced pluripotent stem cell clone selection device according to claim 1, characterized in that, The scraper (22) has a hollow cavity (221) in the middle.

10. The induced pluripotent stem cell clone selection device according to claim 9, characterized in that, A stirring device (222) is symmetrically arranged on the hollow cavity (221).

Images