Perinatal tissue adherent culture dish
By setting indentation marks and inoculation nets at the bottom of the culture dish and combining them with a ramp design, the problems of tissue floating and cumbersome passage in tissue culture dishes are solved, achieving stable cell inoculation and simplifying operations, thus improving observation accuracy and experimental efficiency.
Patent Information
- Application Number
- CN202422934275.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-28
- Publication Date
- 2025-12-05
- Estimated Expiration
- 2034-11-28
AI Technical Summary
In existing perinatal tissue culture dishes, tissues tend to float, cells cannot adhere stably, passage procedures are cumbersome and easily damaged, and the lack of regional division makes observation inconvenient.
A culture dish body with indentation markings was designed, which includes an inoculation net and a ramp. The inoculation net consists of a frame and a gravity ball. The frame includes a clamping part and an inoculation part. The ramp is used to drain the liquid, and the indentation markings are used to divide the area.
This approach achieves stable seeding and simplified passage of tissues, reduces cell damage, improves observation accuracy and experimental convenience, and reduces the impact of liquid shock on cells.
Smart Images
Figure CN223633381U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to tissue culture device technical field, concretely relates to a perinatal tissue adherent culture dish. BACKGROUND
[0002] The perinatal tissue adherent culture dish is used for culturing tissue samples obtained from perinatal period, such as fetal tissue, placental tissue or other related biological samples and perinatal tissue containing umbilical cord mesenchymal stem cells, and the surface thereof is usually coated with specific substances (such as collagen, fibronectin, etc.), so as to promote the adhesion and growth of biological samples such as umbilical cord mesenchymal stem cells. Through adherent culture, cells can maintain their activity for a long time, which is convenient for studying their physiological processes or carrying out drug screening experiments.
[0003] The culture dish used in the existing laboratory is directly inoculated with tissues on the bottom of the culture dish, and the inoculated tissues are easy to float up, so that cells cannot be stably attached to the bottom surface of the culture medium, thereby affecting the normal growth and differentiation of cells. Moreover, no region is divided, if there are abnormal colonies under the microscope, only the abnormal regions can be located by marking marks on the bottom of the culture dish. When subculturing, a pair of tweezers is needed to clamp out the tissues one by one, which is relatively cumbersome, and can easily cause cell damage or introduce contamination. UTILITY MODEL CONTENT
[0004] The utility model discloses a perinatal tissue adherent culture dish.
[0005] The utility model discloses the following technical scheme in order to realize the above-mentioned purpose, comprising:
[0006] The culture dish main part is divided into a plurality of regions through the indentation mark on the bottom.
[0007] The culture dish main part is internally provided with an inoculation net, and the inoculation net comprises a frame and a gravity ball.
[0008] As a further description of the above technical scheme, the culture dish main part is provided with a culture dish cover on the top, and the culture dish cover is used for sealing the culture dish main part.
[0009] As a further description of the above technical scheme, the inner side end face of the culture dish main part is provided with a slope body, and the slope body is used for draining liquid.
[0010] As a further description of the above technical scheme, the inclination angle of the slope body is 45-75 DEG.
[0011] The indentation mark ring is provided with 6-12 groups.
[0012] As a further description of the above technical solution, the frame comprises a clamping portion and an inoculation portion, and the clamping portion is connected to the inoculation portion at the bottom.
[0013] As a further description of the above technical solution, the inoculation portion of the frame is parallel to the inner wall of the culture dish body.
[0014] As a further description of the above technical solution, the inoculation portion comprises a side wall and a bottom wall, and the material of the inoculation portion is nylon mesh.
[0015] As a further description of the above technical solution, a gravity ball is arranged on the inner side end face of the inoculation portion, and the gravity ball is used for applying gravity.
[0016] As a further description of the above technical solution, the gravity ball is symmetrically arranged in 2-4 groups.
[0017] The beneficial effects of the present application are as follows:
[0018] 1. The culture dish body is provided with an inoculation net on the inner side, and the tissue can be inoculated in the inoculation net. When subculturing, the inoculation net can be taken out directly by clamping with hemostatic forceps. The tissue can be separated by one operation, and the inoculation net can be directly moved into a new culture dish, which is more convenient.
[0019] 2. The culture dish body is provided with a slope body, so that the added liquid can flow along the slope and flow at a slower speed, and the impact on the tissue is smaller.
[0020] 3. The culture dish body is divided into multiple areas by pressure mark marks at the bottom, so that the position of the abnormal colony can be directly recorded, and subsequent searching is facilitated.
[0021] In order to more clearly illustrate the structural features and effects of the present application, the present application will be described in detail below in combination with the drawings and specific embodiments. BRIEF DESCRIPTION OF DRAWINGS
[0022] Fig. 1 is a structure schematic view of the perinatal tissue adherent culture dish of the present application;
[0023] Fig. 2 is a top view schematic view of the culture dish body of the present application.
[0024] REFERENCE SIGNS
[0025] 1. culture dish body; 11, pressure mark mark; 12, slope body; 2, culture dish cover; 3, inoculation net; 31, frame; 311, clamping portion; 312, inoculation portion; 3121, side wall; 3122, bottom wall; 32, gravity ball. DETAILED DESCRIPTION
[0026] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings.
[0027] like Figs. 1-2 As shown, in one embodiment, a perinatal tissue adherent culture dish includes: a culture dish body 1, and a culture dish cap 2 is provided on the top of the culture dish body 1 for sealing the culture dish body 1.
[0028] Furthermore, the bottom of the petri dish body 1 is divided into multiple areas by indentation marks 11; specifically, there are 6-12 groups of indentation marks 11.
[0029] Existing petri dishes typically have flat bottoms without any division or markings. When researchers observe cell growth under a microscope, if abnormal cell colonies or irregular growth patterns are found, they often have to manually mark these abnormal areas with a marker. This manual marking method is not only susceptible to interference or obscuring, but the marking positions may also be imprecise, especially in cases of long culture times and dense cell growth. The markings may lose their original accuracy due to cell migration or growth, thus affecting subsequent observation and experimental procedures. The petri dish designed in this application features indentation marking areas on the bottom. Researchers can directly use these indentation areas to mark the locations of abnormal cell colonies, eliminating the need for imprecise marking with a marker and reducing the complexity and error of manual operations.
[0030] Furthermore, a ramp 12 is provided on the inner end face of the culture dish body 1 for guiding liquid flow. Specifically, the inclination angle of the ramp 12 is 45-75°. Existing culture dishes typically have flat bottoms, and once liquid is poured in, it tends to distribute rapidly and evenly, resulting in a relatively fast liquid flow rate. This can potentially impact cells or tissues in culture, especially during tissue culture, cell seeding, or reagent addition. Rapidly flowing liquid can easily cause cell detachment, morphological changes, and even affect experimental results. In this application, after the liquid is added to the culture dish, it first flows gradually along the ramp to the bottom of the culture dish, rather than spreading evenly across the entire bottom surface. Because the liquid flow rate is significantly reduced, it not only effectively reduces the direct impact of the liquid on cells or tissues but also avoids uneven cell distribution or tissue damage caused by rapid liquid flow.
[0031] like Figs. 1-2 As shown, in this embodiment, an inoculation net 3 is provided inside the petri dish body 1, and the inoculation net 3 includes a frame 31 and a gravity ball 32.
[0032] The inoculation net 3 greatly simplifies the inoculation, subculture and transplantation process of the tissue. The tissue can be directly inoculated in the inoculation net 3 during the culture process. The net structure cooperates with the gravity ball 32 to effectively hold the tissue so that the tissue cannot easily float or fall off, and the liquid flow is also avoided to interfere with the position of the tissue. The inoculation net 3 provides a stable support to ensure better contact between the tissue and the culture medium, which is beneficial to the growth and development of the tissue.
[0033] Further, the frame 31 includes a clamping part 311 and an inoculation part 312, and the bottom of the clamping part 311 is connected to the inoculation part 312. The inoculation part 312 of the frame 31 is parallel to the inner wall of the culture dish body 1. Specifically, the inoculation part 312 includes a side wall 3121 and a bottom wall 3122, and the material of the inoculation part 312 is a nylon grid. The inner side end face of the inoculation part 312 is provided with a gravity ball 32 for applying gravity. Specifically, the gravity ball 32 is symmetrically provided with 2-4 groups.
[0034] During the subculture operation, the experimental personnel only need to clamp the top of the inoculation net 3 by using the hemostatic forceps to take out the inoculation net 3 as a whole from the original culture dish, which reduces unnecessary damage or pollution caused by manual operation, and improves the efficiency and accuracy of the subculture. If it is necessary to transplant the tissue to a new culture dish, the experimental personnel only need to move the inoculation net 3 into the new culture dish, which avoids repeated inoculation operation and further improves the convenience of the experiment.
[0035] Working principle:
[0036] Open the culture dish cover 2, and place the washed and chopped tissue block on the inoculation net 3. After standing for 20 minutes, the tissue is naturally adhered to the nylon grid at the bottom of the inoculation net 3. A pipette is used to suck the culture medium and add it along the side edge of the culture dish body 1. The added culture medium will enter the culture dish body 1 through the inclined body 12 and infiltrate into the tissue block through the nylon grid on the inoculation net 3. Finally, the culture dish cover 2 is closed, and the inoculated tissue is placed in a culture box for culture.
[0037] After 3-5 days of culture, the cell growth situation can be observed under a microscope (the cells crawling out of the tissue will enter the bottom of the culture dish body 1 along the nylon grid at the bottom of the inoculation net 3 and grow at the bottom). According to the indentation mark 11 at the bottom of the culture dish body 1, the position of the colony is recorded, which is convenient for finding the original colony during subsequent observation. When changing the liquid, the culture dish cover 2 is opened, the culture dish body 1 is inclined, the culture supernatant is sucked out by using a pipette, and then new culture medium is added along the inclined body 12.
[0038] After 12-14 days of culture, when the cells can be passaged, the frame 31 in the seeding net 3 can be clamped by a hemostatic forceps, then the whole seeding net 3 is taken out, so that the tissue and cells are separated, the culture supernatant is subsequently sucked out, and the digestion and passaging are carried out according to the conventional operation.
[0039] The above description of disclosed embodiments enables one of ordinary skill in the art to make or use the application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein can be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. A perinatal tissue adherent culture dish, characterized in that, Include: The culture dish body (1) is divided into multiple areas by indentation marks (11) at the bottom; The culture dish body (1) is provided with an inoculation net (3) inside, which comprises a frame (31) and a gravity ball (32).
2. The peri-placental tissue adherence petri dish of claim 1, wherein, The culture dish body (1) is provided with a culture dish cover (2) at the top, which is used to seal the culture dish body (1).
3. The peri-placental tissue adherence petri dish of claim 1, wherein, The inner side of the culture dish body (1) is provided with a slope body (12), which is used to drain liquid.
4. The peri-placental tissue adherence culture dish of claim 3, wherein, The inclination angle of the slope body (12) is 45-75°.
5. The peri-placental tissue adherence petri dish of claim 1, wherein, The indentation marks (11) are annularly arranged in 6-12 groups.
6. The peri-postnatal tissue adherence petri dish of claim 1, wherein, The frame (31) comprises a clamping part (311) and an inoculation part (312), and the bottom of the clamping part (311) is connected with the inoculation part (312).
7. The peri-postnatal tissue adherence petri dish of claim 1, wherein, The inoculation part (312) of the frame (31) is parallel to the inner wall of the culture dish body (1).
8. The peri-placental tissue adherence petri dish of claim 6, wherein, The inoculation part (312) comprises a side wall (3121) and a bottom wall (3122), and the material of the inoculation part (312) is nylon grid.
9. The peri-placental tissue adherence petri dish of claim 6, wherein, The inner side of the inoculation part (312) is provided with a gravity ball (32), which is used to apply gravity.
10. The peri-placental tissue adherence petri dish of claim 7, wherein, The gravity ball (32) is symmetrically arranged in 2-4 groups.