Quadruple positioning type eye choroid standardized sample preparation device

Abstract

The utility model discloses a quadruple positioning type standardized sample preparation device for an eye choroid. The quadruple positioning type standardized sample preparation device comprises a base, a positioning column assembly, a quadruple petal preparation knife assembly and a quadruple cutting ring assembly, a positioning column groove, a petal making cutter groove and a cutting ring groove are formed in the front surface of the base; the positioning column assembly comprises a guide column and a positioning column; the quadruple sectioning cutter assembly comprises a sectioning cutter guide ring, a sectioning cutter chassis and four sectioning cutters; the quadruple cutting ring assembly comprises a cutting ring guide ring, a cutting ring chassis and four annular cutting edges; the quadruple valve making knife assembly and the quadruple cutting ring assembly are sequentially installed on the base through positioning and guiding of the positioning column assembly, and quadruple positioning, tiling cutting and standardized sample preparation of choroid tissue are achieved. According to the scheme, through a modular positioning cutting structure, the core problems of poor sample uniformity, low operation efficiency, large tissue damage and the like in the prior art are systematically solved, and a high-reliability and high-stability standardized sample preparation tool is provided for research on eye microcirculation angiogenesis.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of medical equipment required by eye tissue research, especially to a four -in -one positioning formula eye choroid standardization sample preparation device. BACKGROUND

[0002] Microvascular angiogenesis (MVA) is a necessary biological process for human growth and development and tissue damage repair, and abnormal MVA is the pathological basis for the occurrence and development of many eye vascular diseases, such as diabetic retinopathy, retinopathy of prematurity and age-related macular degeneration. Through scientific research on MVA, its pathogenesis and key regulatory molecules can be clarified, which helps to develop new strategies for clinical treatment.

[0003] At present, the choroid sprouting model is an important means to study eye MVA: choroid is a layer of eye tissue rich in microvessels, and the model is obtained by isolating choroid (in practice, retinal pigment epithelium-choroid-sclera tissue) and culturing in vitro in culture medium to observe the effect of different interventions on choroid MVA, so as to explore the occurrence mechanism and intervention strategy of eye MVA.

[0004] At present, the preparation of choroid tissue is mainly through manual micro-operation, that is, under a microscope, eye micro-instruments (microscopical scissors, microforceps) are used for empirical tissue cutting. This method has some problems to be solved:

[0005] (1) The tissue area is not uniform. Previous studies have shown that the in vitro MVA growth rate of choroid tissue in different areas is significantly different, and the rate decreases from the peripheral part to the posterior part.

[0006] (2) The shape of the tissue is inconsistent. The size and shape of the choroid tissue prepared by manual micro-operation are obviously different.

[0007] (3) The in vitro time is inconsistent. Too slow operation will cause too large difference in in vitro time between different tissues, and thus cause different tissue activities.

[0008] (4) The integrity of the tissue is damaged. Micro-instruments will inevitably damage the prepared tissue, such as clamping by microforceps during operation.

[0009] (5) The risk of contamination is increased. Too many instruments and operations will increase the risk of tissue contamination. The above problems will cause differences in tissue samples between different groups, affecting the control setting and the accuracy of the research results in scientific research. How to standardize the preparation of tissue to improve the reliability of research results and make different researches have certain reference value to each other has become a problem to be solved. Technical Field

[0010] In view of the poor uniformity of experimental samples in eye tissue research, which affects the reliability of research results and the comparability of cross-studies, the technical object of the present application is to provide a quadruple positioning eye choroid standardization sample preparation device for standardized and automated preparation of choroid tissue.

[0011] The technical solution adopted by the present application to solve the technical problem is:

[0012] The quadruple positioning eye choroid standardization sample preparation device comprises:

[0013] The base is provided with a positioning column groove in the center of the front face, and two flap-making knife grooves and four ring knife grooves are arranged around the positioning column groove;

[0014] The positioning column assembly comprises a guide column fixed vertically to the side face of the base and a detachable positioning column; the head of the positioning column is conical, for penetrating the choroid tissue and being locked in the positioning column groove;

[0015] The quadruple flap-making knife assembly comprises a flap-making knife guide ring, a flap-making knife base plate and four flap-making knives; the flap-making knife base plate is provided with a flap-making knife positioning column insertion hole matched with the positioning column in the center; the blades of the four flap-making knives are cross-shaped, with an intersection angle of 90°, and the handles are fixed to the flap-making knife base plate; the flap-making knife guide ring is fixed to one side of the flap-making knife base plate, and the ring opening diameter is matched with the guide column, which can move up and down when being sleeved on the column body;

[0016] The quadruple ring knife assembly comprises a ring knife guide ring, a ring knife base plate and four ring-shaped blades; the ring knife base plate is provided with a ring knife positioning column insertion hole matched with the positioning column in the center; the four ring-shaped blades are cross-symmetrically distributed and fixed to the ring knife base plate; the ring knife guide ring is fixed to one side of the ring knife base plate, and the ring opening diameter is matched with the guide column, which can move up and down when being sleeved on the column body;

[0017] The quadruple flap-making knife assembly and the quadruple ring knife assembly are sequentially installed on the base through the positioning and guidance of the positioning column assembly, so as to realize the quadruple positioning, flat cutting and standardization sample preparation of the choroid tissue.

[0018] In a further preferred technical solution, the flap-making knife grooves are straight grooves, and the two flap-making knife grooves are cross-symmetrically distributed; the ring knife grooves are annular grooves, and the four ring knife grooves are cross-symmetrically distributed.

[0019] In a further preferred technical solution, the guide column is hollow inside, the positioning column can be placed in the guide column, an opening is arranged at the top of the guide column, and a cap is arranged on the opening.

[0020] Further preferred technical solutions, the disc blade disc is also provided with four disc blade insertion hole, cross distribution in the four around the disc blade positioning column insertion hole; the disc blade handle is fixed in the disc blade insertion hole.

[0021] Further preferred technical solutions, the ring cutter disc is also provided with four ring cutter screw hole, cross distribution in the four around the disc blade positioning column insertion hole; the head of the ring blade is a ring blade, the tail is a bolt tail, and the bolt tail is fixed in the ring cutter screw hole.

[0022] Further preferred technical solutions, the disc blade is integrally formed, including blade and handle, the blade is made of 316L stainless steel, the blade body and the handle are inclined at an angle of 15°, the blade thickness is 1.2mm, and the intersection of the four blade extension lines coincides with the center of the positioning column groove.

[0023] Further preferred technical solutions, the head of the ring blade is a hollow cylindrical shape, the inner diameter is 1mm, the outer diameter is 1.2mm, and the blade thickness is 0.1mm; the screw thread of the bolt tail and the ring cutter screw hole of the ring cutter disc is engaged at a depth of 3-5mm, and the thread surface is coated with an anti-loosening glue layer.

[0024] Further preferred technical solutions, the bottom surface of the base is provided with an anti-skid silica gel layer, the thickness of the anti-skid silica gel layer is 2-3mm, the surface is distributed with honeycomb-shaped grooves, the groove depth is 0.5-1mm, and the pitch is 3mm.

[0025] The beneficial effects of the utility model are:

[0026] 1. Standardized positioning and cutting

[0027] The precise matching of the positioning column and the base groove, combined with the cross-symmetrical distribution design of the four-cutter, ensures the positional consistency of the posterior segment tissue during fixation and cutting, eliminates regional differences (such as the peripheral part and the posterior polar part), and realizes the high uniformity of sample size (1mm round piece) and shape (four-leaf clover flat).

[0028] 2. Efficient step-by-step operation

[0029] The modular design of the split four-cutter assembly and the ring cutter assembly, through the positioning column quick switching, shortens the tissue off-body time to seconds, reduces the activity loss, at the same time simplifies the operation process, improves the experimental efficiency.

[0030] 3. High-precision cutting to protect tissue activity

[0031] The disc blade of the disc blade is inclined at an angle of 15°, and the cutting trajectory is 90°. The ring blade is designed as a hollow cylindrical shape. This non-contact cutting avoids damage caused by forceps clamping, and the ultra-thin design of the blade reduces tissue extrusion, maintains sample integrity and cell activity.

[0032] 4. Stable anti-skid and anti-deviation

[0033] Multi-stage thread structure of positioning column; anti-skid lines on inner wall of incision hole of flap-making knife; anti-skid silica gel layer on bottom surface of base; such multi-stage locking mechanism prevents displacement of tissue or knife during operation, and the honeycomb-shaped anti-skid layer enhances stability of the device to ensure deviation-free cutting path.

[0034] 5. Expandability and compatibility

[0035] Groove size can be adapted to different knives; cross-symmetrical distribution of incision hole supports expansion of multiple specifications of knives, and compatibility with different experimental requirements (e.g., mouse / human eye tissue) is achieved by replacing knife components (e.g., adjusting the inner diameter of the ring knife), thereby improving the application range of the device.

[0036] 6. Reducing pollution risk

[0037] Integrated plug-in design of flap-making knife and ring knife; reducing the number of instrument replacement, avoiding the introduction of pollutants by multiple operations, meeting the requirements of sterile experiments.

[0038] Summary: This patent systematically solves the core problems of poor sample uniformity, low operation efficiency, and large tissue damage in existing technologies through modular positioning and cutting structure (four-knife assembly + split base), precise mechanical design (groove limiting, anti-skid lines, multi-stage thread), and non-contact cutting technology, providing a high-reliability, high-stability standardized sample preparation tool for eye microcirculation blood vessel neovascularization research. BRIEF DESCRIPTION OF DRAWINGS

[0039] Figure 1 is a structural schematic diagram of the base and positioning column assembly

[0040] Figure 2 is a structural schematic diagram of the four-knife assembly.

[0041] Figure 3 is a connection schematic diagram of the flap-making knife and flap-making knife base.

[0042] Figure 4 is a structural schematic diagram of the four-ring knife assembly.

[0043] Figure 5 is a connection schematic diagram of the ring knife blade and ring knife base.

[0044] Figure 6 is a working state schematic diagram of the four-knife assembly.

[0045] Figure 7 is a working state schematic diagram of the four-ring knife assembly.

[0046] In the figure: 1-base, 2-quadruple valve-making knife assembly, 3-quadruple ring knife assembly, 4-positioning column assembly, 5-posterior segment of eyeball tissue; 101-positioning column groove, 102-valve-making knife groove, 103-ring knife groove; 201-valve-making knife guide ring, 202-valve-making knife base plate, 203-valve-making knife; 301-ring knife guide ring, 302-ring knife base plate, 303-four ring-shaped knife blades; 401-guide column, 402-positioning column, 403-cap; 2021-valve-making knife positioning column insertion hole, 2022-valve-making knife insertion hole; 2031-knife blade, 2032-knife handle; 3021-ring knife positioning column insertion hole, 3022-ring knife screw hole, 3031-ring-shaped knife blade, 3032-bolt tail. DETAILED DESCRIPTION

[0047] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.

[0048] The embodiment completely describes the structural design and use process of the quadruple positioning type eye choroid standard sample preparation device, and specifically as follows:

[0049] I. Device structure

[0050] The structure of the base 1 and the positioning column assembly 4 is shown in Figure 1

[0051] The base 1 is a circular aluminum alloy base plate with a diameter of 120 mm, and the front surface is processed with:

[0052] The positioning column groove 101: the center position, the inner diameter is 8 mm, the depth is 5 mm, and the conical inner wall (30° cone angle);

[0053] The valve-making knife groove 102: including two straight grooves, cross-distributed, groove width 1.2 mm, depth 2.5 mm;

[0054] The ring knife groove 103: four groups of ring grooves, cross-symmetrically distributed, inner diameter 1 mm, outer diameter 1.5 mm, depth 1.5 mm.

[0055] The bottom surface of the base 1 is provided with an anti-slip silica gel layer 104 with a thickness of 2.5 mm, and the surface honeycomb groove 1041 has a depth of 0.8 mm.

[0056] The positioning column assembly 4: including the guide column 401 vertically fixed on the side surface of the base 1 and the detachable positioning column 402;

[0057] ​The bottom of the guide column 401 is fixed on the side of the base 1, which is hollow inside, and the positioning column 402 can be placed in it, and the top is provided with a cap 403.

[0058] The head of the positioning column 402 is a 30° conical shape, which is used to penetrate the choroid tissue and lock in the positioning column groove 101.

[0059] The quadruple flap-making knife assembly 2, as shown in Figure 2 and 3 , includes a flap-making knife guide ring 201, a flap-making knife base 202, and four flap-making knives 203.

[0060] The flap-making knife guide ring 201 is fixed on one side of the flap-making knife base 202, and the ring opening diameter matches the guide column 401, which can move up and down on the column body.

[0061] The flap-making knife base 202 is centrally provided with a flap-making knife positioning column insertion hole 2021 matching the positioning column 402, and four flap-making knife insertion holes 2022 are distributed in a cross shape around it.

[0062] The flap-making knife 203 is integrally formed, including a blade 2031 and a handle 2032, and the four flap-making knives 203 are distributed in a cross symmetry. The handle 2032 is inserted into the flap-making knife insertion hole 2022 for fixation, and the blade 2031 is made of 316L stainless steel, with a 15° inclined blade body and a 90° cross angle.

[0063] The quadruple ring knife assembly 3, as shown in Figure 4 and 5 , includes a ring knife guide ring 301, a ring knife base 302, and four ring-shaped knife blades 303.

[0064] The ring knife guide ring 301 is fixed on one side of the ring knife base 302, and the ring opening diameter matches the guide column 401, which can move up and down on the column body.

[0065] The ring knife base 302 is centrally provided with a ring knife positioning column insertion hole 3021 matching the positioning column 402, and four ring knife screw holes 3022 are provided in a cross shape around it.

[0066] The head of the ring-shaped knife blade 303 is a ring-shaped knife blade 3031, which is made of 316L stainless steel, and the tail is a bolt tail 3032, which is fixed in the ring knife screw hole 3022. The four ring-shaped knife blades 3031 are distributed in a cross symmetry, corresponding to and matching the ring knife groove 103. The blade inner diameter is 1mm, the thickness is 0.1mm, and the blade edge roughness Ra≤0.4μm.

[0067] II. Operation process

[0068] Tissue fixation: Place the posterior segment of the eyeball in the center of the base, and the positioning column 402 penetrates the tissue and is locked in the positioning column groove 101;

[0069] Valve cutting: As shown in Figure 6 , install the four-leaf valve cutter assembly 2, press down the valve cutter bottom plate 202 to cut along the valve cutter groove 102, and form a four-leaf clover-shaped flat tissue;

[0070] Ring sampling: As shown in Figure 7 , replace the four-leaf ring cutter assembly 3, press down the ring cutter bottom plate 302, and the cutting edge cuts into the ring cutter groove 103 to limit the depth, and obtain 4 circular samples with a diameter of 1mm;

[0071] Sample collection: Remove the base and take out the sample for culture.

[0072] In this operation, compared with the traditional method, the following technical advantages exist:

[0073] The matching design of cross-symmetrical cutters and grooves (structural features) ensures that the cutting path is not deviated, and the sample diameter error is ≤2%;

[0074] The anti-slip silicone layer (structural feature) prevents the base from sliding during operation, improving cutting accuracy.

[0075] Operation efficiency: single sample preparation time is 25 seconds, which is increased by 86% compared with manual operation (3 minutes).

[0076] It should be noted that in this text, terms such as "including", "containing" or any other variants thereof are intended to cover non-exclusive inclusion, so that the process, method, article or device including a series of elements not only includes those elements, but also includes other elements not explicitly listed, or includes elements inherent to such process, method, article or device. Without more limitations, the element defined by the statement "including a…" does not exclude the presence of another identical element in the process, method, article or device including the element.

[0077] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirits of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A quadruple positioning standardized sample preparation device for eye choroid, characterized in that, The utility model relates to a four-connection choroid tissue cutting device, comprising: a base with a positioning column groove in the center of the front face, two flap-making knife grooves and four ring knife grooves around the positioning column groove; a positioning column assembly, including a guide column fixed vertically on the side of the base and a detachable positioning column; the head of the positioning column is conical, used for penetrating choroid tissue and locking in the positioning column groove; a four-connection flap-making knife assembly, including a flap-making knife guide ring, a flap-making knife base and four flap-making knives; the flap-making knife base is centrally provided with a flap-making knife positioning column insertion hole matched with the positioning column; the blades of the four flap-making knives are cross-shaped, with a cross angle of 90 degrees; the handles of the flap-making knives are fixed on the flap-making knife base; the flap-making knife guide ring is fixed on one side of the flap-making knife base, with a ring opening diameter matched with the guide column, and can move up and down when sleeved on the column body; a four-connection ring knife assembly, including a ring knife guide ring, a ring knife base and four ring blades; the ring knife base is centrally provided with a ring knife positioning column insertion hole matched with the positioning column; the four ring blades are cross-symmetrically distributed and fixed on the ring knife base; the ring knife guide ring is fixed on one side of the ring knife base, with a ring opening diameter matched with the guide column, and can move up and down when sleeved on the column body; the four-connection flap-making knife assembly and the four-connection ring knife assembly are sequentially installed on the base through the positioning and guiding of the positioning column assembly, to realize four-connection positioning, flat cutting and standardized sample preparation of choroid tissue.

2. The quadruple positioning eye choroid standardization sampling device according to claim 1, wherein, the flap-making knife grooves are straight-line grooves, and the two flap-making knife grooves are cross-symmetrically distributed; the ring knife grooves are ring grooves, and the four ring knife grooves are cross-symmetrically distributed.

3. The quadruple positioning eye choroid standardization sampling device according to claim 1, wherein, the guide column is hollow inside, and the positioning column can be placed in the guide column; the top of the guide column is provided with an opening and is equipped with a cap.

4. The quadruple positioning eye choroid standardization sampling device according to claim 1, wherein, the flap-making knife base is further provided with four flap-making knife insertion holes, which are cross-symmetrically distributed around the flap-making knife positioning column insertion hole; the handles of the flap-making knives are fixed in the flap-making knife insertion holes.

5. The quadruple positioning eye choroid standardization sampling device according to claim 1, wherein, the ring knife base is further provided with four ring knife screw holes, which are cross-symmetrically distributed around the flap-making knife positioning column insertion hole; the head of the ring blade is a ring blade, and the tail is a bolt tail, which is fixed in the ring knife screw hole.

6. The quadruple positioning eye choroid standardization sampling device according to claim 5, wherein, the head of the ring blade is a hollow cylindrical shape, with an inner diameter of 1 mm, an outer diameter of 1.2 mm and a blade thickness of 0.1 mm; the screw thread engagement depth of the bolt tail and the ring knife screw hole of the ring knife base is 3-5 mm, and the screw thread surface is coated with an anti-loosening glue layer.

7. The quadruple positioning eye choroid standardization sampling device according to claim 1, wherein, the flap-making knife is integrally formed, including a blade and a handle; the blade is made of 316L stainless steel; the blade body and the handle form an inclination angle of 15 degrees; the blade thickness is 1.2 mm; the intersection point of the four blade extension lines coincides with the center of the positioning column groove.

8. The quadruple positioning eye choroid standardization sampling device according to claim 1, wherein, the bottom surface of the base is provided with an anti-slip silica gel layer, the thickness of the anti-slip silica gel layer is 2-3 mm, the surface of the anti-slip silica gel layer is distributed with honeycomb grooves, the groove depth is 0.5-1 mm, and the interval is 3 mm.

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