Full-automatic affinity chromatography process control system
The fully automated affinity chromatography process control system utilizes liquid aspiration and dissipation devices and electric valve actuators to achieve automated liquid operation, solving the problems of low efficiency and insufficient accuracy caused by manual operation, and realizing efficient and accurate liquid control and detection results.
Patent Information
- Application Number
- CN202423238522.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-26
- Publication Date
- 2025-12-09
- Estimated Expiration
- 2034-12-26
AI Technical Summary
The current affinity chromatography process involves a high degree of manual operation, resulting in low efficiency and difficulty in controlling the liquid volume and flow rate, which affects the accuracy of the detection results.
Design a fully automated affinity chromatography process control system that automatically controls liquid flow through a liquid suction and discharge device. The system includes a main pipeline, chromatography column, filtration device, liquid inlet system, collection system, and first and second liquid suction and discharge devices. The automated operation of the liquid is achieved using valves and electric actuators.
Reduce human intervention, improve the uniformity and accuracy of liquid volume and flow rate control, and enhance the precision of test results.
Smart Images
Figure CN223641370U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the technical field of chromatography, and particularly relates to a full-automatic affinity chromatography process control system. BACKGROUND
[0002] Affinity chromatography, also known as affinity chromatography, is a separation and purification technology based on specific interaction between biomolecules. This method is based on the combination of affinity materials on the stationary phase and the substances to be separated in the mobile phase. This combination is reversible, and the two can be separated from each other when the mobile phase conditions are changed.
[0003] Using this technology, affinity chromatography is usually used to purify or concentrate a certain or a certain type of substance from a liquid mixture. Similarly, it can also be used to remove or reduce the content of a certain or a certain type of substance in the mixture.
[0004] Mycotoxins are a class of substances widely present in food, feed, medicinal materials, etc. Their harm to human health has been widely recognized. Timely detection can help detect and control toxin contamination in a timely manner.
[0005] According to relevant national regulations and industry standards, the current process for detecting toxins in these samples is basically three steps: sample pretreatment, toxin extraction, and toxin detection. Among them, the current toxin extraction is usually operated manually, and the operation process is as follows:
[0006] In the affinity chromatography process, the sample is passed through the chromatography column by using a syringe, and the binding liquid is added to the syringe. After the pump flow controller controls the washing of the affinity chromatography column, the eluent is added to the syringe, and the pump flow controller controls the elution of the affinity chromatography column. The eluted sample flows into the collection bottle. After elution, the binding liquid is added to the syringe, and the pump flow controller controls the cleaning of the pipeline and the affinity chromatography column. It can be seen that the proportion of manual operation in the whole process is very high. This not only has low efficiency, but also the amount and flow rate of manual liquid delivery are difficult to control, which will have a great impact on the results. Utility model content
[0007] The utility model aims at providing a full-automatic affinity chromatography process control system, which can efficiently and automatically control the flow of liquid in the pipeline and reduce the errors caused by manual operation.
[0008] The utility model discloses a full -automatic affinity chromatography process control system, including main pipeline, set up chromatography column on main pipeline, set up filter equipment on main pipeline, set up liquid inlet system at one end of main pipeline, set up collection system at the other end of main pipeline, with first suction and discharge liquid device that main pipeline is connected to with second suction and discharge liquid device that main pipeline is connected to, first suction and discharge liquid device set up between liquid inlet system with chromatography column, second suction and discharge liquid device set up between chromatography column with collection system, filter equipment set up between first suction and discharge liquid device with chromatography column.
[0009] Further, the liquid inlet system comprises a liquid storage bottle connected with the main pipeline, and a first valve arranged between the liquid storage bottle and the main pipeline.
[0010] Further, the liquid storage bottle is provided with a pair of first four-way pipes, and the pair of liquid storage bottles are connected with the first four-way pipe.
[0011] Further, the first four-way pipe is further connected with an air pipe, and the first valve is arranged on the air pipe.
[0012] Further, the collection system comprises a pair of collection bottles and a second four-way pipe arranged at the end of the main pipeline, and the pair of collection bottles are connected with the second four-way pipe.
[0013] Further, the first suction and discharge liquid device comprises a shell, a syringe detachably arranged in the shell, a catheter connected with the nipple of the syringe, a push plate arranged in the shell, and an electric push rod connected with the push plate.
[0014] Further, the shell is provided with a pair of clamping grooves, and the finger hook part of the syringe is clamped in the clamping grooves.
[0015] Further, the structure of the second suction and discharge liquid device is the same as that of the first suction and discharge liquid device.
[0016] Further, a pressure gauge is arranged on the main pipeline.
[0017] The technical scheme of the utility model has at least the following advantages and beneficial effects: the full-automatic affinity chromatography process control system of the utility model, when in use, absorbs the combined liquid in the liquid inlet system through the liquid suction and discharge device, and the whole main pipeline is cleaned through the combined liquid (the combined liquid is discharged into the collection system), the sample solution is preloaded in the first liquid suction and discharge device, the sample solution can pass through the chromatography column through the first liquid suction and discharge device, then the combined liquid in the liquid inlet system is absorbed through the first liquid suction and discharge device or the second liquid suction and discharge device to clean the main pipeline and the chromatography column (the combined liquid is discharged into the collection system), then the eluent in the liquid inlet system is absorbed through the first liquid suction and discharge device or the second liquid suction and discharge device, and the eluent passes through the chromatography column, the eluent is discharged into the collection system and is collected separately, finally the first liquid suction and discharge device and the second liquid suction and discharge device are used in sequence to clean the main pipeline and the chromatography column, and the waste liquid after cleaning is discharged into the collection system and is collected temporarily, so that the whole process can be operated through the automatic opening and closing of the valve and the automatic operation of the first liquid suction and discharge device and the second liquid suction and discharge device, manual intervention is extremely rare, the control of the liquid amount, the control of the liquid flow rate and the accuracy of the result in the whole process can be more unified, the accuracy is higher, and the result is more accurate. BRIEF DESCRIPTION OF DRAWINGS
[0018] Figure 1 The full-automatic affinity chromatography process control system provided by the utility model embodiment is shown in the structure diagram.
[0019] Figure 2 The structure diagram of the first liquid suction and discharge device provided by the utility model embodiment is shown.
[0020] Icon: 11-main pipeline, 12-first four-way pipe, 13-second four-way pipe, 14-chromatography column, 15-filter, 16-pressure gauge, 20-first liquid suction and discharge device, 21-housing, 22-syringe, 23-piston, 24-push plate, 25-electric push rod, 26-clamping groove, 27-finger hooking part, 28-nipple, 29-catheter, 210-third valve, 30-second liquid suction and discharge device, 40-liquid inlet system, 41-liquid storage bottle, 42-first valve, 43-air pipe, 44-filtering device, 50-collection system, 51-collection bottle, 52-second valve. DETAILED DESCRIPTION
[0021] EMBODIMENT
[0022] The following is further illustrated in combination with specific embodiments, such as the accompanying drawings Figure 1 -APPENDIX Figure 2As shown, the fully automated affinity chromatography process control system of this embodiment includes a main pipeline 11, a chromatography column 14 disposed on the main pipeline 11, a filter device disposed on the main pipeline 11, a liquid inlet system 40 disposed at one end of the main pipeline 11, a collection system 50 disposed at the other end of the main pipeline 11, a first liquid suction and discharge device 20 connected to the main pipeline 11, and a second liquid suction and discharge device 30 connected to the main pipeline 11; the first liquid suction and discharge device 20 is disposed between the liquid inlet system 40 and the chromatography column 14, the second liquid suction and discharge device 30 is disposed between the chromatography column 14 and the collection system 50, and the filter device is disposed between the first liquid suction and discharge device 20 and the chromatography column 14. Specifically, during use, the binding liquid in the inlet system 40 is absorbed by the aspiration and dissipation device, and the binding liquid is used to clean the entire main pipeline 11 (the binding liquid is discharged into the collection system 50). The first aspiration and dissipation device 20 is pre-filled with sample solution, and the sample solution can be passed through the chromatography column 14 by the first aspiration and dissipation device 20. Then, the first aspiration and dissipation device 20 or the second aspiration and dissipation device 30 is used to absorb the binding liquid in the inlet system 40 to clean the main pipeline 11 and the chromatography column 14 (the binding liquid is discharged into the collection system 50). Then, the first aspiration and dissipation device 20 or the second aspiration and dissipation device 30 is used to absorb the eluent in the inlet system 40 and pass the eluent through the chromatography column 14. The eluent is discharged into the collection system 50 and collected separately. Finally, the first aspiration and dissipation device 20 and the second aspiration and dissipation device 30 are used in sequence to clean the main pipeline 11 and the chromatography column 14. In this way, the entire process can be operated automatically by the opening and closing of the valves and the automatic operation of the first suction and discharge device 20 and the second suction and discharge device 30, requiring very little manual intervention. This makes the control of liquid volume and liquid flow rate more uniform, more precise, and more accurate.
[0023] The liquid feeding system 40 in this embodiment includes a storage bottle 41 connected to the main pipeline 11, and a first valve 42 disposed between the storage bottle 41 and the main pipeline 11. A pair of storage bottles 41 are provided, and a first four-way pipe 12 is provided at the end of the main pipeline 11. Both storage bottles 41 are connected to the first four-way pipe 12, and a first valve 42 is provided at the connection point between the two storage bottles 41 and the first four-way pipe 12. Specifically, the pair of storage bottles 41 are used to hold the binding solution and the eluent, respectively. When absorbing the binding solution, the first valve 42 on the eluent storage bottle 41 is closed. After the liquid is drawn into the first suction and discharge device 20, all the first valves 42 are closed. Then, the first suction and discharge device 20 expels the binding solution, allowing it to pass through the chromatography column 14. The same applies to the eluent. When using the second suction / discharge device 30, the liquid has already passed through the chromatography column 14 when the second suction / discharge device 30 draws liquid. After closing all the second valves 52, the liquid discharged from the second suction / discharge device 30 can be directly discharged into the collection system 50. Alternatively, both the inlet system 40 and the collection system 50 can be closed, and then the first suction / discharge device 20 and the second suction / discharge device 30 can be started simultaneously. When the first suction / discharge device 20 draws liquid, the second suction / discharge device 30 discharges liquid, and vice versa. This allows the bound liquid to flow back and forth multiple times within the chromatography column 14, which can better clean the main pipeline 11 and the chromatography column 14.
[0024] In this embodiment, the first four-way pipe 12 is also connected to an air pipe 43, and the air pipe 43 is equipped with a first valve 42; the air pipe 43 is equipped with a filter device 44. Specifically, by absorbing air through the air pipe 43, all the liquid in the main pipe 11 can be drained.
[0025] The collection system 50 in this embodiment includes a pair of collection bottles 51 and a second four-way pipe 13 located at the end of the main pipe 11. Both collection bottles 51 are connected to the second four-way pipe 13, and a second valve 52 is provided at each connection point between the collection bottles 51 and the second four-way pipe 13. A second suction / drainage device 30 is connected to the second four-way pipe 13. Specifically, the pair of collection bottles 51 are used to receive waste bound liquid and eluent, respectively. When the first suction / drainage device 20 or the second suction / drainage device 30 absorbs liquid, both second valves 52 need to be closed. When draining liquid, the corresponding second valve 52 needs to be opened.
[0026] The first aspiration and drainage device 20 in this embodiment includes a housing 21, a syringe 22 detachably disposed within the housing 21, a conduit 29 connected to the nipple 28 of the syringe 22, a push plate 24 disposed within the housing 21, and an electric push rod 25 connected to the push plate 24. The push plate 24 is fixedly connected to the piston 23 rod of the syringe 22, and the conduit 29 is connected to the main pipe 11, with a third valve 210 provided on the conduit 29. Specifically, the aspiration and drainage operation of the first aspiration and drainage device 20 is mainly completed by the syringe 22. The electric push rod 25 pushes the push plate 24 to move the piston 23 of the syringe 22, thereby achieving the purpose of aspiration and drainage. The syringe 22 is used because of its good safety, low cost, and ease of replacement. Furthermore, the syringe 22 needs to be removed, the sample solution drawn, and then reinserted into the housing 21.
[0027] In this embodiment, the housing 21 is provided with a pair of slots 26, and the finger hook 27 of the syringe 22 is engaged in the slots 26. Specifically, the finger hook 27 of the syringe 22 is engaged in the slots 26, and then the push plate 24 is connected to the piston 23 of the syringe 22, and the nipple 28 of the syringe 22 is connected to the catheter 29.
[0028] The structure of the second liquid suction and discharge device 30 in this embodiment is the same as that of the first liquid suction and discharge device 20.
[0029] In this embodiment, a pressure gauge 16 is installed on the main pipe 11.
[0030] In summary, the fully automated affinity chromatography process control system of this embodiment uses a suction and discharge device to absorb the binding liquid in the inlet system 40, and uses the binding liquid to clean the entire main pipeline 11 (the binding liquid is discharged into the collection system 50). The first suction and discharge device 20 is pre-filled with sample solution, which allows the sample solution to pass through the chromatography column 14. Then, the first suction and discharge device 20 or the second suction and discharge device 30 is used to absorb the binding liquid in the inlet system 40 to clean the main pipeline 11 and the chromatography column 14 (the binding liquid is discharged into the collection system 50). Then, the first suction and discharge device 20 or the second suction and discharge device 30 is used to absorb the eluent in the inlet system 40 and allow the eluent to pass through the chromatography column 14. The eluent is discharged into the collection system 50 and collected separately. Finally, the first suction and discharge device 20 and the second suction and discharge device 30 are used sequentially to clean the main pipeline 11 and the chromatography column 14. In this way, the entire process can be operated automatically by the opening and closing of the valves and the automatic operation of the first suction and discharge device 20 and the second suction and discharge device 30, requiring very little manual intervention. This makes the control of liquid volume and liquid flow rate more uniform, more precise, and more accurate.
[0031] The above are merely preferred embodiments of this utility model and are not intended to limit the scope of this utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of this utility model should be included within the protection scope of this utility model.
Claims
1. A fully automated affinity chromatography process control system, characterized in that: Includes a main pipe (11), a chromatography column (14) disposed on the main pipe (11), a filtration device disposed on the main pipe (11), a liquid inlet system (40) disposed at one end of the main pipe (11), a collection system (50) disposed at the other end of the main pipe (11), a first liquid suction and discharge device (20) connected to the main pipe (11), and a second liquid suction and discharge device (30) connected to the main pipe (11); The first liquid aspiration device (20) is located between the liquid inlet system (40) and the chromatography column (14), the second liquid aspiration device (30) is located between the chromatography column (14) and the collection system (50), and the filtration device is located between the first liquid aspiration device (20) and the chromatography column (14).
2. The fully automated affinity chromatography process control system according to claim 1, characterized in that: The liquid inlet system (40) includes a storage bottle (41) connected to the main pipe (11) and a first valve (42) disposed between the storage bottle (41) and the main pipe (11).
3. The fully automated affinity chromatography process control system according to claim 2, characterized in that: The liquid storage bottle (41) is provided in pairs, and the end of the main pipe (11) is provided with a first four-way pipe (12). The liquid storage bottle (41) of the pair is connected to the first four-way pipe (12), and a first valve (42) is provided at the connection between the liquid storage bottle (41) of the pair and the first four-way pipe (12).
4. The fully automated affinity chromatography process control system according to claim 3, characterized in that: The first four-way pipe (12) is also connected to an air pipe (43), on which the first valve (42) is provided; and on which a filter device (44) is provided.
5. The fully automated affinity chromatography process control system according to claim 1, characterized in that: The collection system (50) includes a pair of collection bottles (51) and a second four-way pipe (13) located at the end of the main pipe (11); Each of the two collection bottles (51) is connected to the second four-way pipe (13), and a second valve (52) is provided at the connection between each of the two collection bottles (51) and the second four-way pipe (13); the second suction and discharge device (30) is connected to the second four-way pipe (13).
6. The fully automated affinity chromatography process control system according to claim 1, characterized in that: The first suction and discharge device (20) includes a housing (21), a syringe (22) detachably disposed in the housing (21), a conduit (29) connected to the nipple (28) of the syringe (22), a push plate (24) disposed in the housing (21), and an electric push rod (25) connected to the push plate (24); The push plate (24) is fixedly connected to the piston (23) rod of the syringe (22), the conduit (29) is connected to the main pipe (11), and a third valve (210) is provided on the conduit (29).
7. The fully automated affinity chromatography process control system according to claim 6, characterized in that: The housing (21) is provided with a pair of slots (26), and the finger hook (27) of the syringe (22) is engaged in the slots (26).
8. The fully automated affinity chromatography process control system according to claim 7, characterized in that: The structure of the second liquid suction and discharge device (30) is the same as that of the first liquid suction and discharge device (20).
9. The fully automated affinity chromatography process control system according to claim 1, characterized in that: A pressure gauge (16) is installed on the main pipeline (11).