Flow cytometry sample preservation device for anoist apoptosis

Abstract

The utility model discloses a flow cytometry sample preservation device for anoidosis, which comprises a refrigeration box body and a refrigeration box cover hinged to the upper end of the refrigeration box body, a partition plate is arranged in the refrigeration box body, the internal space of the refrigeration box body is divided into an upper cavity and a lower cavity by the partition plate, a plurality of mounting holes are formed in the partition plate in a penetrating manner, and the upper cavity and the lower cavity are communicated through the mounting holes. A test tube rack for positioning a test tube is inserted into the mounting hole, the lower end of the test tube rack extends into the lower cavity, a sealing cover is detachably mounted at the upper end of the test tube rack, and the sealing cover is detachably connected with the upper end of the mounting hole. The test tube storage device can prevent excessive loss of cold air in the device when a target test tube is taken out, and avoids influence on storage of other test tube cell samples.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell sample storage device technical field, concretely relates to a flow cytometry sample storage device for anoikis. BACKGROUND

[0002] Anoikis is a special programmed cell death induced by cell detachment from extracellular matrix or adjacent cell contact. The process is triggered by mitochondrial disturbance to activate Bim and Fas / Fas-L mediated Caspase cascade, and its regulation involves integrin alphaVbeta3, EGFR, PI3K-AKT and other signaling pathways. In tumor metastasis, cancer cells acquire anoikis resistance through activation of ADAM10-p75NTR ICD-TRAF6-NF-kappa B, C / EBPbeta-PDGFB and other pathways, promoting peritoneal and ovarian metastasis. Studies have shown that this mechanism is closely related to the prognosis of patients with gastric cancer, breast cancer and other diseases, and the related molecular markers have clinical evaluation value. The core principle of flow cytometry for detecting cell anoikis is to quantitatively analyze the specific changes (such as cell membrane phosphatidylserine evagination, DNA fragmentation, etc.) that occur during apoptosis. At present, the flow cytometry sample storage device for anoikis in the laboratory usually uses a conventional medical refrigerator, and the refrigerator is built-in test tube rack, and the flow cytometry sample is stored in the test tube and positioned in the test tube rack of the refrigerator.

[0003] However, when the target test tube is taken out, the test tubes on the test tube rack are completely exposed, and the external hot air directly enters the refrigerator for heat exchange, which will cause excessive loss of cold air in the refrigerator, affecting the preservation of other test tube cell samples, therefore, it is necessary to improve the existing flow cytometry sample storage device to solve the above problems. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a flow cytometry sample storage device for anoikis, which can prevent excessive loss of cold air in the device when the target test tube is taken out, and avoid affecting the preservation of other test tube cell samples.

[0005] To achieve the above purpose, the utility model provides the following technical scheme: a flow cytometry sample storage device for anoikis, comprising a refrigerator body and a refrigerator cover hinged to the upper end of the refrigerator body, a partition plate is arranged in the refrigerator body, the partition plate divides the internal space of the refrigerator body into an upper cavity and a lower cavity, a plurality of mounting holes are provided through the partition plate, a test tube rack for positioning test tubes is inserted into the mounting holes, the lower end of the test tube rack extends into the lower cavity, a cover is detachably mounted on the upper end of the test tube rack, and the cover and the upper end of the mounting hole are detachably connected together.

[0006] By adopting the technical scheme, first, the refrigeration area is separated by the partition plate; when the refrigeration box cover is opened, the upper cavity is exposed to the external air, and the lower cavity is isolated; when the target test tube rack is taken out, only a small mounting hole is exposed on the partition plate, and only a small amount of cold air in the lower cavity will flow out to the outside through the mounting hole; after the test tubes on the target test tube rack are taken away, the test tube rack is inserted into the mounting hole again, and the cover is connected to the upper end of the mounting hole to block it, and then the refrigeration box cover is covered, thereby, the loss of cold air in the device caused by taking out the target test tube is minimized, so that the preservation of other test tube cell samples is not affected.

[0007] The utility model discloses further set up, the test tube frame includes the bottom plate, the multiple connecting rods that are distributed on the bottom plate on the circumference array and are set up the connecting screw bushing on the multiple connecting rods upper end, the cover lower extreme is equipped with the connecting stud for the close contact test tube cover upper end, the connecting stud with the connecting screw bushing thread cooperation.

[0008] By adopting the technical scheme, the test tube rack structure is light, the side hollow area is large, the cold air is convenient for the quick cooling of the test tube, and the test tube rack and the cover are connected through the thread cooperation mode, and the dismounting operation is very convenient.

[0009] The utility model discloses further set up, the connecting stud lower extreme is equipped with the first positioning slot, the first positioning slot is equipped with the first elastic pad for the close contact test tube cover upper end on the interference installation.

[0010] By adopting the technical scheme, when the cover is screwed on the upper end of the test tube rack, the first elastic pad is tightly contacted with the upper end of the test tube cover, which prevents the test tube from vibrating up and down when the device is carried, thereby ensuring the stability of the test tube positioning structure.

[0011] The utility model discloses further set up, the inside of connecting rod is equipped with the multiple second positioning slots along the verticality, the second positioning slot is equipped with the second elastic pad for the close contact test tube outer wall on the interference installation.

[0012] By adopting the technical scheme, the second elastic pad is arranged on the inside of the test tube rack to tightly contact the outer wall of the test tube, which further improves the stability of the test tube mounting structure.

[0013] The utility model discloses further set up, the partition plate is equipped with an installation seat at the position of the upper end of each mounting hole, the installation seat is equipped with the installation groove that is used for embedding the cover, and the outer circular surface of the cover is equipped with at least one lock block, the inner circular surface of the installation groove is equipped with at least one lock groove matched with the lock block, the lock groove includes the arc locking portion that extends peripherally, and the access slide that extends downward from the upper end of the installation seat and is communicated with one end of the arc locking portion.

[0014] By adopting the technical scheme, the locking and fixing of the cover in the installation groove or the disengagement of the cover from the installation groove can be realized through two actions of "pushing (pulling) + rotating", and the dismounting operation is very convenient.

[0015] The utility model further sets up, the arc locking portion gradually reduces in height in the direction away from the access slide, and when the lock block slides to one end of the arc locking portion away from the access slide, the lock block and the arc locking portion are interference fit.

[0016] By adopting the technical scheme, the locking and fixing of the cover in the installation groove or the disengagement of the cover from the installation groove can be realized through two actions of "pushing (pulling) + rotating", and the dismounting operation is very convenient.

[0017] The utility model further sets up, the arc locking portion gradually reduces in height in the direction away from the access slide, and when the lock block slides to one end of the arc locking portion away from the access slide, the lock block and the arc locking portion are interference fit.

[0018] By adopting the technical scheme, the locking and fixing of the cover in the installation groove or the disengagement of the cover from the installation groove can be realized through two actions of "pushing (pulling) + rotating", and the dismounting operation is very convenient.

[0019] The utility model further sets up, the arc locking portion gradually reduces in height in the direction away from the access slide, and when the lock block slides to one end of the arc locking portion away from the access slide, the lock block and the arc locking portion are interference fit.

[0020] By adopting the technical scheme, the locking and fixing of the cover in the installation groove or the disengagement of the cover from the installation groove can be realized through two actions of "pushing (pulling) + rotating", and the dismounting operation is very convenient.

[0021] The utility model further sets up, the arc locking portion gradually reduces in height in the direction away from the access slide, and when the lock block slides to one end of the arc locking portion away from the access slide, the lock block and the arc locking portion are interference fit.

[0022] By adopting the technical scheme, the locking and fixing of the cover in the installation groove or the disengagement of the cover from the installation groove can be realized through two actions of "pushing (pulling) + rotating", and the dismounting operation is very convenient. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 It is the sectional view of the utility model whole;

[0024] Figure 2 It is the structure schematic view of the utility model test tube and cover cooperation;

[0025] Figure 3 It is the structure schematic view of the utility model test tube and cover cooperation;

[0026] Figure 4 It is the structure schematic view of the utility model test tube and cover cooperation;

[0027] Figure 5The structure diagram of the cover is separated from the mounting groove.

[0028] In the figure: 1, refrigeration box body; 2, refrigeration box cover; 3, partition; 4, upper cavity; 5, lower cavity; 6, mounting hole; 7, test tube; 8, test tube rack; 9, cover; 10, bottom plate; 11, connecting rod; 12, connecting screw sleeve; 13, test tube cover; 14, connecting stud; 15, first positioning groove; 16, first elastic pressing block; 17, second positioning groove; 18, second elastic pressing block; 19, mounting seat; 20, mounting groove; 21, locking block; 22, locking groove; 23, arc-shaped locking portion; 24, access slide; 25, annular clamping groove; 26, elastic ring; 27, linear operation protrusion; 28, guide sleeve. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0030] Embodiment: a flow cytometry sample storage device for anoikis as shown in the accompanying drawings, Figures 1 to 5 The utility model discloses a kind of for anoikis flow cytometry sample storage device, including refrigeration box body 1 and the refrigeration box cover 2 hinged to the upper end of refrigeration box body 1, the basic structure of refrigeration box is the conventional medical refrigeration box structure in market, it is conventional structure, thus its specific structure is not detailed here, the partition 3 is located in the refrigeration box body 1, partition 3 can be integrated with the inner wall of refrigeration box body 1 structure or clamping structure or be connected by fastener, the partition 3 will the inside space of the refrigeration box body 1 be divided into upper cavity 4 and lower cavity 5, the height of lower cavity 5 is much greater than the height of upper cavity 4, the partition 3 is provided with a plurality of mounting holes 6, test tube rack 8 for positioning test tube 7 is inserted in the mounting hole 6, i. e. test tube 7 is loaded into test tube rack 8, the lower end of test tube rack 8 extends into lower cavity 5, detachable cover 9 is installed on the upper end of test tube rack 8, cover 9 is detachably connected with the upper end of mounting hole 6, cover 9 can block mounting hole 6. First, utilize partition 3, refrigeration area is divided, when refrigeration box cover 2 is opened, upper cavity 4 is exposed to external air, lower cavity 5 is isolated, when target test tube rack 8 is taken out, only a small mounting hole 6 is exposed on partition 3, and only a small amount of cold air in lower cavity 5 will flow out to the outside through mounting hole 6, after the test tube 7 on target test tube rack 8 is taken away, test tube rack 8 is inserted into mounting hole 6 again, cover 9 is connected to the upper end of mounting hole 6, and it is blocked, and refrigeration box cover 2 is covered again, thereby, the loss of cold air in the device caused by taking out target test tube 7 is minimized, so that the preservation of other test tube 7 cell samples is not affected.

[0031] As shown in the accompanying drawings Figures 2 to 4 The tube rack 8 includes a base plate 10, a plurality of connecting rods 11 arranged in a circumferential array on the base plate 10, and a connecting sleeve 12 provided at the upper end of the connecting rods 11. The lower end of the cover 9 is provided with a connecting stud 14 for abutting against the upper end of the tube cover 13, and the connecting stud 14 is threadedly connected with the connecting sleeve 12. The tube rack 8 is light in structure and has a large hollow area on the side (i.e. the part between two adjacent connecting rods 11), which facilitates the cold air to pass through to quickly cool the tubes 7. Meanwhile, the tube rack 8 is connected with the cover 9 through thread connection, which is very convenient for disassembly and assembly.

[0032] As shown in the accompanying drawings Figures 2 to 4 The lower end of the connecting stud 14 is provided with a first positioning groove 15, and a first elastic pressing block 16 for abutting against the upper end of the tube cover 13 is fitted in the first positioning groove 15. The two parts can be reinforced by glue, and the lower end of the first elastic pressing block 16 protrudes out of the first positioning groove 15. When the cover 9 is screwed onto the upper end of the tube rack 8, the first elastic pressing block 16 abuts against the upper end of the tube cover 13, preventing the tubes 7 from vibrating up and down during the transportation of the device, thereby ensuring the stability of the positioning structure of the tubes 7.

[0033] As shown in the accompanying drawings Figures 2 to 4 The inner side of the connecting rod 11 is provided with a plurality of second positioning grooves 17 in the vertical direction, and a second elastic pressing block 18 for abutting against the outer wall of the tube 7 is fitted in the second positioning groove 17. The two parts can be reinforced by glue, and the outer end of the second elastic block protrudes out of the second positioning groove 17, and the outer end of the second elastic block has a dome structure. The inner side of the tube rack 8 is provided with the second elastic pressing block 18 to abut against the outer wall of the tube 7, further improving the stability of the installation structure of the tube 7.

[0034] The first elastic pressing block 16 and the second elastic pressing block 18 can both be made of rubber.

[0035] As shown in the accompanying drawings Figure 5 The partition plate 3 is provided with an installation seat 19 corresponding to the position of the upper end of each installation hole 6. The installation seat 19 is provided with an installation groove 20 for embedding the cover 9, and the outer circular surface of the cover 9 is provided with at least one lock block 21. The inner circular surface of the installation groove 20 is provided with at least one locking groove 22 matched with the lock block 21. The locking groove 22 includes an arc-shaped locking part 23 extending in the circumferential direction, and an access slide 24 extending downward from the upper end of the installation seat 19 and connected with one end of the arc-shaped locking part 23. Through the two actions of "pushing (pulling) + rotating", the cover 9 can be locked and fixed in the installation groove 20 or separated from the installation groove 20, which is very convenient for disassembly and assembly.

[0036] In this design, the arc-shaped locking part 23 gradually decreases in height away from the slide rail 24, and when the locking block 21 slides to the end of the arc-shaped locking part 23 away from the slide rail 24, the locking block 21 and the arc-shaped locking part 23 are in an interference fit. This design prevents the locking block 21 from loosening when it slides into the inner end of the arc-shaped locking part 23, effectively improving the stability of the cover 9 after locking.

[0037] As attached Figure 5 As shown, an annular groove 25 is provided on the inner end face of the mounting groove 20. An elastic ring 26 is embedded in the annular groove 25 for abutting against the lower end face of the cap 9. The elastic ring 26 can be made of rubber. The elastic ring 26 increases the friction force on the lower end of the cap 9, thereby improving the stability of the locking structure of the cap 9, and thus improving the firmness of the installation of the test tube rack 8 and the test tube 7.

[0038] As attached Figure 5 As shown, the upper end of the cap 9 is integrally provided with a straight operating protrusion 27, which facilitates the operator to rotate the cap 9 and lift the cap 9 and the test tube rack 8.

[0039] As attached Figure 1 As shown, a guide sleeve 28 is provided at the inner end of each mounting hole 6 corresponding to the partition 3. The guide sleeve 28 is fitted around the outer periphery of the test tube rack 8, and the height of the guide sleeve 28 is less than half the height of the test tube rack 8. This design can guide the installation of the test tube rack 8, ensuring stable up and down movement of the test tube rack 8 containing test tubes 7 when it is inserted into or removed from the mounting hole 6, without causing collisions with other test tubes 7.

Claims

1. A flow cytometry sample preservation device for anopyroptosis, comprising a refrigerator body (1) and a refrigerator lid (2) hinged to the upper end of the refrigerator body (1), characterized in that: The refrigerator body (1) is provided with a partition (3), which divides the internal space of the refrigerator body (1) into an upper cavity (4) and a lower cavity (5). Multiple mounting holes (6) are provided through the partition (3). A test tube rack (8) for positioning test tubes (7) is inserted into the mounting holes (6). The lower end of the test tube rack (8) extends into the lower cavity (5). A cap (9) is detachably installed on the upper end of the test tube rack (8). The cap (9) is detachably connected to the upper end of the mounting hole (6).

2. The flow cytometry sample preservation device for anopyroptosis according to claim 1, characterized in that: The test tube rack (8) includes a base plate (10), a plurality of connecting rods (11) arranged in a circumferential array on the base plate (10), and connecting sleeves (12) provided on the upper ends of the plurality of connecting rods (11). The lower end of the cover (9) is provided with a connecting stud (14) for pressing against the upper end of the test tube cover (13). The connecting stud (14) is threadedly engaged with the connecting sleeve (12).

3. The flow cytometry sample preservation device for anopyroptosis according to claim 2, characterized in that: The lower end of the connecting stud (14) is provided with a first positioning groove (15), and a first elastic pressure block (16) for pressing against the upper end of the test tube cap (13) is interference-fitted on the first positioning groove (15).

4. The flow cytometry sample preservation device for anopyroptosis according to claim 2, characterized in that: The inner side of the connecting rod (11) is provided with a plurality of second positioning grooves (17) in the vertical direction, and a second elastic pressure block (18) for pressing against the outer wall of the test tube (7) is interference-fitted on the second positioning groove (17).

5. A flow cytometry sample preservation device for anopyroptosis according to claim 4, characterized in that: The partition (3) is provided with a mounting base (19) at the upper end of each mounting hole (6). The mounting base (19) is provided with a mounting groove (20) for the cover (9) to be inserted. The outer circular surface of the cover (9) is provided with at least one locking block (21). The inner circular surface of the mounting groove (20) is provided with at least one locking groove (22) that cooperates with the locking block (21). The locking groove (22) includes an arc-shaped locking part (23) extending circumferentially and an inlet / outlet slide (24) extending downward from the upper end of the mounting base (19) and communicating with one end of the arc-shaped locking part (23).

6. A flow cytometry sample preservation device for anopyroptosis according to claim 5, characterized in that: The height of the arc-shaped locking part (23) gradually decreases in the direction away from the inlet / outlet slide (24), and when the locking block (21) slides to one end of the arc-shaped locking part (23) away from the inlet / outlet slide (24), the locking block (21) and the arc-shaped locking part (23) are in an interference fit.

7. A flow cytometry sample preservation device for anopyroptosis according to claim 5, characterized in that: The mounting groove (20) has an annular groove (25) on its inner end face, and an elastic ring (26) is embedded in the annular groove (25) for abutting against the lower end face of the cover (9).

8. A flow cytometry sample preservation device for anopyroptosis according to claim 5, characterized in that: The upper end of the cover (9) is provided with a straight-line operation protrusion (27).

9. A flow cytometry sample preservation device for anopyroptosis according to claim 1, characterized in that: The partition (3) is provided with a guide sleeve (28) at the inner end of each mounting hole (6). The guide sleeve (28) is fitted around the outer periphery of the test tube rack (8), and the height of the guide sleeve (28) is less than half the height of the test tube rack (8).

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