Microscope visual field positioning auxiliary device for observing culture plate

Abstract

The utility model provides a microscope field positioning auxiliary device for observing a culture plate, which comprises a shell, a clamping groove, a transverse positioning rule, a longitudinal positioning rule, a scale and a sliding track, the sliding track is arranged among a fixed pulley, an auxiliary fixed pulley and a calibration pulley, and the fixed pulley, the auxiliary fixed pulley and the calibration pulley are all mounted on the shell. A transverse positioning ruler and a longitudinal positioning ruler are arranged on the sliding crawler belt, a ruler is fixed on the shell through a ruler fixing shaft, and clamping grooves are formed in the bottom corners of the periphery of the shell and used for fixing a culture plate needing to be observed. The device is simple to operate, the visual field of a microscope can be accurately recorded as coordinate information, and after a culture plate is fixed on the device, the initial positions of all visual field information are kept uniform, so that the repeatability among different experiment batches is facilitated. When a user fails to record adjustment data due to negligence, the adjustment data can be recorded by referring to the position of the scale, and omission of experimental data is avoided.

Description

Technical Field

[0001] This utility model relates to the field of microscope observation technology, and in particular to a microscope field of view positioning auxiliary device for observing culture plates. Background Technology

[0002] In studying tumor cell migration ability, the cell scratch assay is often the preferred method due to its ease of operation. In this assay, cells are seeded in a culture dish, and once they have covered the bottom, a 10 μL pipette tip is used to vertically scratch the center of each well. The width of the scratched area is continuously observed and images are captured. Analysis of these width changes assesses changes in cell migration ability, thus determining the impact of treatment strategies on cell migration. Therefore, only a fixed microscope field of view ensures accurate and reliable results.

[0003] However, due to the delicate handling required under a microscope, even slight shaking can lead to deviations in the observation results. Some experiments require periodic observation of the same area over a certain period of time, which requires researchers to accurately record the observation position under the microscope during repeated experiments; current technology cannot easily place the culture plate into the preset position. Utility Model Content

[0004] Therefore, to solve the above problems, this utility model provides a microscope field-of-view positioning auxiliary device for observing culture plates. This device can fix the culture plate in the same initial position, and by rotating the calibration screw, it records the observation position under the microscope and provides a reference for the measurement data. The technical means adopted by this utility model are as follows:

[0005] A microscope field-of-view positioning auxiliary device for observing culture plates includes a housing, a slot, a transverse positioning ruler, a longitudinal positioning ruler, a scale, and a sliding track. The sliding track is disposed between a fixed pulley and a calibration pulley, both of which are mounted on the housing. The transverse positioning ruler and the longitudinal positioning ruler are disposed on the sliding track. The scale is fixed to the housing via a scale fixing shaft. The slot is disposed at the bottom corners of the housing for fixing the culture plate to be observed.

[0006] Furthermore, it also includes auxiliary fixed pulleys, which are installed on the housing. Auxiliary fixed pulleys are provided on the corner side of both the fixed pulley and the calibration pulley near the housing. They are used to make the sliding track fit more closely to the edge of the housing, so that the initial positions of the lateral positioning ruler and the longitudinal positioning ruler are at the edge of the housing.

[0007] Furthermore, there are two calibration pulleys, one positioned along the length and the other along the width of the housing. Specifically, one is located at the leftmost end along the length, and the other at the end of the width that is closest to the calibration pulley along the length. This ensures that, in the initial state, the lateral and longitudinal positioning rulers are positioned close to the "origin," facilitating adjustment. There are six fixed pulleys, two positioned on the same side of the housing as the calibration pulleys along the length and width, and the other four positioned on the opposite side of the housing along the length and width, respectively, to allow the sliding track to pass over them.

[0008] Furthermore, the shell has a length of 12-13cm, a width of 8-10cm, and a height of 2-3cm.

[0009] Furthermore, the sliding track has a transmission length of 12-13cm and a width of 1-2cm.

[0010] Furthermore, the calibration pulley has a calibration range of 12-13cm, a coarse calibration screw accuracy of 1cm, and a fine calibration screw accuracy of 0.1cm.

[0011] Furthermore, the smallest division of the scale is 0.1 cm.

[0012] Furthermore, the distance between the two reference axes of the horizontal and vertical positioning rulers is 0.1 cm, and there are a total of 21 reference axes.

[0013] This invention has the following advantages: It is simple to operate and can accurately record the microscope field of view as coordinate information. Furthermore, after fixing the culture plate to the device, the initial position of all field of view information will remain consistent, which helps to ensure repeatability between different experimental batches. Since the positioning ruler is mainly positioned by adjusting the calibration screw, if the user fails to record the adjustment data due to negligence, the ruler position can be used as a reference, avoiding the omission of experimental data. Simultaneously, when measuring the spacing of images under the microscope, the user can determine the distance to be measured based on the number of reference axes crossed by the spacing. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a top view of the overall structure of this utility model;

[0016] Figure 2This is a front view schematic diagram of the overall structure of this utility model;

[0017] Figure 3 This is a schematic diagram of the overall left-side structure of this utility model;

[0018] In the diagram: 1. Fixed pulley; 2. Sliding track; 3. Scale; 4. Calibration pulley; 5. Longitudinal positioning scale; 6. Lateral positioning scale; 7. Housing; 8. Auxiliary fixed pulley; 9. Scale fixing shaft; 10. Slot. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0020] like Figures 1-3 As shown in the figure, this utility model embodiment discloses a microscope field of view positioning auxiliary device for observing culture plates, including a housing 7, a slot 10, a transverse positioning ruler 6, a longitudinal positioning ruler 5, a scale 3, and a sliding track 2. The sliding track is disposed between a fixed pulley, an auxiliary fixed pulley, and a calibration pulley. The fixed pulley 1 and the calibration pulley 4 are both mounted on the housing. A transverse positioning ruler and a longitudinal positioning ruler are disposed on the sliding track. The scale is fixed to the housing through a scale fixing shaft 9. The slot is disposed at the bottom corners of the housing for fixing the culture plate to be observed.

[0021] Furthermore, it also includes an auxiliary fixed pulley 8, which is installed on the housing. An auxiliary fixed pulley is provided on the corner side of both the fixed pulley and the calibration pulley near the housing. It is used to make the sliding track fit more closely with the edge of the housing, so that the initial position of the lateral positioning ruler and the longitudinal positioning ruler is at the edge of the housing.

[0022] Furthermore, there are two calibration pulleys, one positioned along the length and the other along the width of the housing. Specifically, one is located at the leftmost end along the length, and the other at the end of the width that is closest to the calibration pulley along the length. This ensures that, in the initial state, the lateral and longitudinal positioning rulers are positioned close to the "origin," facilitating adjustment. There are six fixed pulleys, two positioned on the same side of the housing as the calibration pulleys along the length and width, and the other four positioned on the opposite side of the housing along the length and width, respectively, to allow the sliding track to pass over them.

[0023] Furthermore, the shell has a length of 12-13cm, a width of 8-10cm, and a height of 2-3cm.

[0024] Furthermore, the sliding track has a transmission length of 12-13cm and a width of 1-2cm. In this embodiment, the transmission length of the sliding track refers to the distance that the sliding track can move.

[0025] Furthermore, the calibration pulley has a calibration range of 12-13cm, the coarse calibration screw has an accuracy of 1cm, and the fine calibration screw has an accuracy of 0.1cm. Specifically, the calibration screw performs coarse and fine calibration based on its accuracy; one rotation of the coarse calibration screw moves the sliding track 1cm, and one rotation of the fine calibration screw moves the sliding track 0.1cm.

[0026] Rotating the coarse and fine calibration screws will cause the sliding track to move. The horizontal and vertical positioning rulers are fixed on the sliding track, so they will move forward together.

[0027] Furthermore, the smallest division of the scale is 0.1 cm.

[0028] Furthermore, the distance between the two reference axes of the horizontal and vertical positioning rulers is 0.1 cm, and there are a total of 21 reference axes.

[0029] In practice, the experimenter fixes the culture plate to be observed in slot 10 and places it under a microscope to observe microorganisms. At this time, there is no scale. First, the optimal magnification for observation is selected by adjusting the microscope's field of view, and then the selected field of view is photographed. First, the coarse calibration screw on calibration pulley 4 is adjusted to move the horizontal and vertical positioning scales 5 and 6 to the vicinity of the selected field of view, and the rotation scale is recorded. Then, the fine calibration screw is adjusted under the microscope so that the reference axis on the horizontal and vertical positioning scales 5 and 6 completely covers the selected field of view. The number of rotations of the coarse and fine calibration screws and the corresponding movement distance of scale 3 are recorded. This locates the field of view, and the position information of the field of view at this time is digitized. Before the next experiment, the horizontal and vertical positioning scales 5 and 6 must be reset to ensure the consistency of experimental observation. That is, rotating the same distance in the next experiment will obtain the same field of view area of ​​the cell culture plate.

[0030] In this embodiment, there are 21 reference axes spaced 0.1 cm apart, which can cover a 2x2 area to ensure the entire selected field of view is covered. At this point, it is not necessary to determine which axes are covered and which are not; simply record the scale readings or the number of rotations. If precise positioning of a point within the field of view is required, it is necessary to move to the selected area and then count the number of reference axes.

[0031] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this utility model.

Claims

1. A microscope field-of-view positioning auxiliary device for observing culture plates, characterized in that, The device includes a housing, a slot, a transverse positioning ruler, a longitudinal positioning ruler, a scale, and a sliding track. The sliding track is positioned between a fixed pulley and a calibration pulley, both of which are mounted on the housing. A transverse positioning ruler and a longitudinal positioning ruler are mounted on the sliding track. The scale is fixed to the housing via a scale fixing shaft. The slot is located at the bottom corners of the housing and is used to fix the culture plate to be observed.

2. The microscope field of view positioning auxiliary device for observing culture plates according to claim 1, characterized in that, It also includes auxiliary fixed pulleys, which are installed on the housing. Auxiliary fixed pulleys are provided on the corner side of both the fixed pulley and the calibration pulley near the housing. They are used to make the sliding track fit more closely to the edge of the housing, so that the initial positions of the lateral positioning ruler and the longitudinal positioning ruler are at the edge of the housing.

3. The microscope field of view positioning auxiliary device for observing culture plates according to claim 1, characterized in that, The number of calibration pulleys is two, which are respectively set in the length and width directions of the housing. The number of fixed pulleys is six, of which two are set in the length and width directions of the housing on the same side as the calibration pulleys, and the other four are respectively located in the length and width directions of the housing on the other side.

4. The microscope field of view positioning auxiliary device for observing culture plates according to claim 1, characterized in that, The shell is 12-13cm long, 8-10cm wide, and 2-3cm high.

5. The microscope field of view positioning auxiliary device for observing culture plates according to claim 1, characterized in that, The sliding track has a transmission length of 12-13cm and a width of 1-2cm.

6. The microscope field of view positioning auxiliary device for observing culture plates according to claim 1, characterized in that, The calibration pulley has a calibration range of 12-13cm, the coarse calibration screw has an accuracy of 1cm, and the fine calibration screw has an accuracy of 0.1cm.

7. The microscope field of view positioning auxiliary device for observing culture plates according to claim 1, characterized in that, The smallest division of the scale is 0.1 cm.

8. The microscope field of view positioning auxiliary device for observing culture plates according to claim 1, characterized in that, The distance between the two reference axes of the horizontal and vertical positioning rulers is 0.1cm, and there are a total of 21 reference axes.

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