Force-controllable cell nucleus removal tube

Abstract

The utility model discloses a force controllable cell nucleus removal pipe, relates to cell experiment technical field, including removal pipe body, the middle of removal pipe body's lower surface passes through and is fixedly connected with a pipeline, the lower part of pipeline's outer surface is provided with a conical surface 1, the upper part of removal pipe body's outer surface is provided with a cylindrical block, the conical surface 1 is provided with a conical surface 2, the cylindrical block is provided with a conical surface 3, and the conical surface 2 is provided with a conical surface 4. A connecting column is slidably connected to the middle of the upper surface of the cylindrical block in a penetrating mode. The connecting column is pressed downwards to drive the connecting assembly to move downwards, the spring is driven to stretch, the pipeline is inserted into a cell to be in contact with a cell nucleus through the cooperation of a microscope, the pressing force on the connecting column is slowly reduced by a worker, and the connecting column is driven to move upwards under the action of the stretched spring; when the connecting assembly moves, cell nucleuses are sucked into the pipeline and enter the removing pipe body under the action of air pressure, so that operation is completed, and the effects that the cell nucleuses are removed, and the force is easy to control are achieved through the arrangement of the structure.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell experiment technical field, concretely relates to a controllable cell nucleus removes the pipe. BACKGROUND

[0002] The cell nucleus is the closed membrane organelle existing in the eukaryotic cell, and the inside contains most of the genetic material in the cell, that is DNA. These DNA and various proteins, such as histone, are complexed to form chromatin. And the chromatin will condense to form chromosomes when the cell divides, and all the genes contained in the chromosomes are collectively called nuclear genes. The role of the cell nucleus is to maintain the integrity of the genes and to affect the cell activity by regulating gene expression. When performing cloning operation in cell experiment, the cell nucleus needs to be removed from the inside of the cytoplasm under the microscope through the action of the cell nucleus removal tube. The existing technology has the following problems:

[0003] In the current cell nucleus removal tube, the cell nucleus is directly sucked by the worker in the pulling mode. Since the pulling mode is unstable and not easy to control, it causes the problem of not being convenient to use. At the same time, since the current cell nucleus removal tube is designed as a whole structure, it causes the problem of not being convenient to disassemble and replace the internal structure. UTILITY MODEL CONTENT

[0004] The utility model provides a controllable cell nucleus removes the pipe to solve the problem in the above background art.

[0005] To solve the above technical problems, the utility model adopts the following technical scheme:

[0006] A controllable cell nucleus removal tube, comprising a removal tube body, a pipe is fixedly connected through the middle of the lower surface of the removal tube body, a conical surface one is arranged below the outer surface of the pipe, a cylindrical block is arranged above the outer surface of the removal tube body, a connecting column is slidably connected through the middle of the upper surface of the cylindrical block, a circular block is fixedly connected through the upper surface of the outer surface of the connecting column, the outer surface of the connecting column extends to the inside of the removal tube body, a threaded sleeve is threadedly connected to the outer surface of the connecting column, a connecting assembly is arranged on the inner wall of the removal tube body, the connecting assembly comprises a rubber plug and a spring, an annular groove is formed in the middle of the outer surface of the rubber plug, a sealing rubber ring is tightly lapped in the inside of the annular groove, the outer surfaces of the rubber plug and the sealing rubber ring are lapped with the inner wall of the removal tube body, and the middle of the upper surface of the rubber plug is fixedly connected with the lower end of the connecting column.

[0007] The further improvement of the utility model technical scheme is that the inside diameter of the sealing rubber ring is smaller than the inner wall diameter of the annular groove.

[0008] The further improvement of the utility model technical scheme lies in that the inside of the spring is sleeved with the outer surface of the connecting column, and the lower end of the spring is fixedly connected with the upper surface of the rubber plug.

[0009] The further improvement of the utility model technical scheme lies in that two positioning rubber rings are fixedly connected above the outer surface of the removal pipe body, and a conical surface two is arranged above the outer surface of each of the two positioning rubber rings.

[0010] The further improvement of the utility model technical scheme lies in that the inside of the cylindrical block is provided with a cylindrical rubber, and the inner wall diameter of the cylindrical rubber is greater than the outer surface diameter of the removal pipe body.

[0011] The further improvement of the utility model technical scheme lies in that the inner wall of the cylindrical rubber is overlapped and tightly contacted with the outer surface of the two positioning rubber rings, the two positioning rubber rings are in a contracted state, and the inner wall of the cylindrical rubber is sleeved with the outer surface of the removal pipe body.

[0012] The further improvement of the utility model technical scheme lies in that the lower surface of the cylindrical block is provided with a circular port, the inner wall of the circular port is fixedly connected with the outer surface of the cylindrical rubber, and the upper surface of the inner wall of the circular port is fixedly connected with the upper end of the spring.

[0013] The further improvement of the utility model technical scheme lies in that the inner wall of the cylindrical rubber is provided with a conical surface three below.

[0014] Due to the adoption of the above technical scheme, the utility model has the following technical progress compared with the prior art:

[0015] 1、The utility model provides a controllable cell nucleus removal pipe, adopt the mutual cooperation of removal pipe body, connecting assembly, rubber plug, annular groove, sealing rubber ring, spring, pipeline, conical surface one, connecting column, circular block, first through the down press connecting column drive connecting assembly moves down to drive spring to stretch, again through the cooperation of microscope inserts the pipeline in cell and contacts with cell nucleus, and through the worker slowly reduces the force of press connecting column, because the spring under the action of stretching drive connecting column slowly moves up, when connecting assembly moves because of the action of air pressure, cell nucleus is absorbed into the pipeline and enters the inside of removal pipe body, so that the operation is completed, through the setting of the structure realizes the removal of cell nucleus, and the effect that the force is easy to control.

[0016] 2. The utility model provides a controllable cell nucleus removes the pipe, adopted cylindrical block, cylindrical rubber, circular mouth, conical surface three, positioning rubber ring, conical surface two mutual cooperation, through directly moving the cylindrical block with force upwards drives cylindrical rubber, makes cylindrical rubber and the outer surface of positioning rubber ring separate, realizes quick disassembly through the setting of this structure, carries out the replacement to the sealing rubber ring in connecting assembly, prevents the sealing rubber ring to have wear and tear under long time use thus has the phenomenon of air leakage, is not convenient to the cell nucleus is inhaled. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is the structure schematic diagram of the utility model;

[0018] Figure 2 It is the front surface cross section structure schematic diagram of the utility model's remove pipe body;

[0019] Figure 3 It is the connecting assembly decomposition and limit sleeve structure schematic diagram of the utility model;

[0020] Figure 4 It is the remove pipe body, cylindrical block decomposition structure schematic diagram of the utility model;

[0021] Figure 5 It is the conical surface three structure schematic diagram in the cylindrical rubber of the utility model.

[0022] In the drawing: 1, remove pipe body; 11, connecting assembly; 111, rubber plug; 112, annular groove; 113, sealing rubber ring; 114, spring; 101, positioning rubber ring; 102, conical surface two; 2, pipe; 3, conical surface one; 4, cylindrical block; 41, cylindrical rubber; 42, circular mouth; 401, conical surface three; 5, connecting column; 6, circular block; 7, threaded sleeve. DETAILED DESCRIPTION

[0023] In order to make the technical means, creative features, purposes and effects realized by the utility model easy to understand, the utility model is further described below in combination with specific implementation manners.

[0024] As Figure 1 The utility model provides a controllable cell nucleus removes the pipe, including remove pipe body 1, the lower surface middle part of remove pipe body 1 is fixedly connected with the pipe 2 through the penetration, the outer surface below of pipe 2 is provided with conical surface one 3, the outer surface top of remove pipe body 1 is provided with cylindrical block 4, the upper surface middle part of cylindrical block 4 is slidably connected with connecting column 5 through the penetration, the outer surface top of connecting column 5 is fixedly connected with circular block 6 through the penetration, the outer surface of connecting column 5 extends to the inside of remove pipe body 1, the outer surface of connecting column 5 is connected with threaded sleeve 7 in screw thread,

[0025] In use, first hold the upper position of the removal tube body 1 by the worker, and press the circular block 6 with the thumb, so as to drive the connecting column 5 to move downward, drive the connecting assembly 11 and the spring 114 to stretch, and move the removal tube body 1 under the microscope. Through the observation of the microscope, the worker inserts the tapered surface one 3 in the pipeline 2 into the cell to contact with the cell nucleus. At the same time, the worker reduces the pressing force of the thumb due to the effect after the stretching of the spring 114, so as to drive the connecting assembly 11 to move upward. When the connecting assembly 11 moves upward, the cell nucleus is sucked into the pipeline 2 and enters the inside of the removal tube body 1 due to the effect of the air pressure, so as to complete the operation. The setting of the threaded sleeve 7 can adjust the distance, so as to adjust the size of the inner cavity of the cell nucleus suction in the inside of the removal tube body 1. When pressing downward, the spring 114 is limited through the block of the threaded sleeve 7.

[0026] As shown in Figure 2 - Figure 3 The inner wall of the removal tube body 1 is provided with the connecting assembly 11. The connecting assembly 11 comprises the rubber plug 111 and the spring 114. The outer surface of the rubber plug 111 is provided with the annular groove 112 in the middle. The inner part of the annular groove 112 is provided with the sealing rubber ring 113. The outer surfaces of the rubber plug 111 and the sealing rubber ring 113 are in abutment with the inner wall of the removal tube body 1. The upper surface of the rubber plug 111 is fixedly connected with the lower end of the connecting column 5. The inner side diameter of the sealing rubber ring 113 is smaller than the inner wall diameter of the annular groove 112. The inside of the spring 114 is sleeved with the outer surface of the connecting column 5. The lower end of the spring 114 is fixedly connected with the upper surface of the rubber plug 111.

[0027] The setting of the rubber plug 111 is convenient for the up and down sliding of the connecting column 5 to drive the position to move. The sealing rubber ring 113 is tightly attached to the inner wall of the removal tube body 1 to achieve the sealing effect. The setting of the annular groove 112 is convenient for the installation and removal of the sealing rubber ring 113. The structure is convenient for the replacement of the sealing rubber ring 113.

[0028] As shown in Figure 4 The outer surface of the removal tube body 1 is fixedly connected with two positioning rubber rings 101. The outer surfaces of the two positioning rubber rings 101 are provided with tapered surfaces two 102. The inside of the cylindrical block 4 is provided with the cylindrical rubber 41. The inner wall diameter of the cylindrical rubber 41 is greater than the outer surface diameter of the removal tube body 1. The inner wall of the cylindrical rubber 41 is in abutment with the outer surfaces of the two positioning rubber rings 101. The two positioning rubber rings 101 are in the contraction state. The inner wall of the cylindrical rubber 41 is sleeved with the outer surface of the removal tube body 1.

[0029] The cylindrical rubber 41 is separated from the outer surface of the positioning rubber ring 101 by directly moving the cylindrical block 4 upward, and the connecting assembly 11 is moved out from the inside of the removal pipe body 1, so that the sealing rubber ring 113 in the connecting assembly 11 is quickly disassembled and replaced, and the outer surface of the positioning rubber ring 101 and the removal pipe body 1 are fixed by glue.

[0030] As shown in Figure 5 The lower surface of the cylindrical block 4 is provided with a circular port 42, the inner wall of the circular port 42 is fixedly connected with the outer surface of the cylindrical rubber 41, the upper surface of the inner wall of the circular port 42 is fixedly connected with the upper end of the spring 114, and the inner wall of the cylindrical rubber 41 is provided with a tapered surface three 401 below;

[0031] The circular port 42 is arranged to facilitate the fixation of the outer surface of the cylindrical rubber 41, and the tapered surface three 401 is arranged to facilitate the installation of the cylindrical block 4 and the removal pipe body 1, that is, when installation is needed, the connecting assembly 11 is placed in the inside of the removal pipe body 1, and the cylindrical block 4 is pressed to drive the cylindrical rubber 41, so that the tapered surface three 401 contacts the outer surface of the tapered surface two 102 in the positioning rubber ring 101, so as to be extruded and shrunk, so that the positioning rubber ring 101 and the cylindrical rubber 41 are deformed, and the two are tightly contacted and positionally limited, so as to achieve the installation effect.

[0032] The working principle of the force-controllable cell nucleus removal pipe will be described below.

[0033] In use, first, a worker holds the upper position of the removal pipe body 1, and presses the circular block 6 with the thumb, so as to drive the connecting column 5 to move downward, drive the connecting assembly 11 to move downward to stretch the spring 114, and move the removal pipe body 1 under the microscope. Through the observation of the microscope, the worker inserts the tapered surface one 3 in the pipe 2 into the cell to contact the cell nucleus, at the same time, the worker reduces the pressing force of the thumb due to the effect of the stretched spring 114, so as to drive the connecting assembly 11 to move upward, and when the connecting assembly 11 moves upward, the cell nucleus is sucked into the pipe 2 and the inside of the removal pipe body 1 due to the effect of the air pressure, so as to complete the operation.

[0034] The above is a detailed description of the general utility model, but some modifications or improvements can be made on the basis of the utility model, which is obvious to those skilled in the art. Therefore, the modifications or improvements without departing from the spirit of the utility model are within the protection scope of the utility model.

Claims

1. A force-controllable nucleus removal pipette comprising a removal pipette body (1), characterized in that: The lower surface of the removal pipe body (1) is fixedly connected with a pipeline (2), the outer surface of the pipeline (2) is provided with a tapered surface (3), the outer surface of the removal pipe body (1) is provided with a cylindrical block (4), the upper surface of the cylindrical block (4) is slidably connected with a connecting column (5), the outer surface of the connecting column (5) is fixedly connected with a circular block (6), the outer surface of the connecting column (5) extends to the inside of the removal pipe body (1), the outer surface of the connecting column (5) is threadedly connected with a threaded sleeve (7), the inner wall of the removal pipe body (1) is provided with a connecting assembly (11), the connecting assembly (11) comprises a rubber plug (111) and a spring (114), the outer surface of the rubber plug (111) is provided with an annular groove (112), the inside of the annular groove (112) is tightly lapped with a sealing rubber ring (113), the outer surfaces of the rubber plug (111) and the sealing rubber ring (113) are lapped with the inner wall of the removal pipe body (1), and the upper surface of the rubber plug (111) is fixedly connected with the lower end of the connecting column (5).

2. The controllable force cell nucleus removal tube according to claim 1, wherein: The inner diameter of the sealing rubber ring (113) is smaller than the inner wall diameter of the annular groove (112).

3. The controllable force cell nucleus removal tube according to claim 1, wherein: The inside of the spring (114) is sleeved with the outer surface of the connecting column (5), and the lower end of the spring (114) is fixedly connected with the upper surface of the rubber plug (111).

4. The controllable force cell nucleus removal tube according to claim 1, wherein: The outer surface of the removal pipe body (1) is fixedly connected with two positioning rubber rings (101), and the outer surfaces of the two positioning rubber rings (101) are provided with tapered surfaces (102).

5. The controllable force cell nucleus removal tube according to claim 1, wherein: The inside of the cylindrical block (4) is provided with a cylindrical rubber (41), and the inner wall diameter of the cylindrical rubber (41) is greater than the outer surface diameter of the removal pipe body (1).

6. The controllable force cell nucleus removal tube according to claim 5, wherein: The inner wall of the cylindrical rubber (41) is tightly lapped with the outer surfaces of the two positioning rubber rings (101), the two positioning rubber rings (101) are in a contracted state, and the inner wall of the cylindrical rubber (41) is sleeved with the outer surface of the removal pipe body (1).

7. The controllable force cell nucleus removal tube according to claim 5, wherein: The lower surface of the cylindrical block (4) is provided with a circular port (42), the inner wall of the circular port (42) is fixedly connected with the outer surface of the cylindrical rubber (41), and the upper surface of the inner wall of the circular port (42) is fixedly connected with the upper end of the spring (114).

8. The controllable force cell nucleus removal tube according to claim 5, wherein: The inner wall of the cylindrical rubber (41) is provided with a tapered surface (401).

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