Automatic enzyme-linked immunosorbent assay device

Abstract

The utility model discloses automatic enzyme-linked immunoassay detection equipment which comprises a processing module and a washing liquid module, and the processing module comprises a pipetting module, a clamping jaw module and an operation processing area, the operation treatment area is provided with a reaction container, a cleaning position, a cleaning head matched with the cleaning position, an oscillation incubation area, a sample adding area, a consumable loading area, a reagent loading area and an enzyme immunoassay detection area, the cleaning head comprises a liquid injection needle and a liquid suction needle, and the pipetting module and the clamping jaw module can be driven to move along the operation treatment area. The pipetting module can transfer a sample liquid or a reagent to the reaction container, the clamping jaw module can move to the reaction container to perform cover opening and closing operation and can transfer the reaction container into a cleaning position, and the washing liquid module can be in fluid communication with the liquid injection needle and the liquid suction needle to complete cleaning of the reaction container. The detection equipment provided by the utility model can realize a series of full-automatic enzyme immunoassay detection such as sample transfer, reagent transfer, enzyme immunoassay reaction, cleaning and enzyme immunoassay detection.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the in-vitro diagnosis technical field, more particularly to an automatic enzyme-linked immunoassay equipment. BACKGROUND

[0002] In the in-vitro diagnosis technology, various types of detection equipment have been developed with antigen antibody as the core raw material, including ELISA, chromatography, histochemistry, chemiluminescence and other types of detection equipment. ELISA detection is the abbreviation of enzyme-linked immunosorbent assay. The corresponding complete determination can be traced back to the article published by Engvall and Perlmann in 1971. For the first time, enzyme-labeled antibody technology was developed into a determination scheme for micro-substances in liquid samples. After decades of technological development, this type of detection method has been increasingly widely used in in-vitro diagnosis. With people's increasing concern about health problems, more and more urgent detection needs drive the rapid development of fully automatic enzyme immunoassay equipment. Automatic equipment has very high processing capacity and can perform efficient parallel processing of multiple samples or multiple projects. However, enzyme immunoassay involves many types of processing steps, which requires more functional modules for the equipment. Therefore, the spatial layout of the equipment is difficult. At the same time, the demand for high-precision functional units for pipetting and transferring makes the system more complex.

[0003] Automated enzyme immunoassay (ELISA) equipment involves various scenarios including pipetting and transfer of reaction containers or consumables. Therefore, the design of pipetting modules based on pipetting units such as air-displacement pipettes and clamping modules based on mechanical grippers is essential. The solution disclosed in Chinese invention patent CN102729244B, published on June 18, 2014, combines grippers and pipetting units to form a functional module with a common span beam and motion drive. To improve pipetting efficiency, multiple pipetting units can be set up, and grippers can be configured simultaneously within the pipetting module. Movement within the same space is achieved through sliders, allowing both to work simultaneously and improving utilization. However, in actual use, the operating frequency of clamping and pipetting is high, and the working stroke ranges of pipetting and clamping are not the same. This increases the driving complexity of the integrated module, and frequent operation reduces the long-term reliability of the drive mechanism and degrades the operating accuracy. Chinese utility model patent CN219496401U, published on August 8, 2023, discloses a scheme for configuring a universal robotic arm within an enzyme immunoassay (ELISA) device. Different functional modules for ELISA are configured around the robotic arm's operating range. While the universal robotic arm offers flexibility, it is more expensive, requires higher control precision, is more complex, and occupies more space. In reality, the real limiting factor in ELISA is not the transfer efficiency and precision of the mechanical grippers, but rather the pipetting efficiency. Chinese invention patent CN107831322A, published on March 23, 2018, defines the structure of the universal robotic arm and limits its operating range to cover multiple areas, including loading the ELISA plate, transferring it to the shaking incubation area after sample addition, transferring it to the washing area after incubation, and the detection area. This represents a more detailed and optimized design. The Chinese invention patent disclosed in CN107831326A, published on March 23, 2018, features two pipetting modules. The first and second modules are used for transferring different liquids, respectively. This segmented design saves time spent by the pipetting arm reciprocating, improving overall efficiency. However, this also increases the complexity of the system due to the increased number of sub-modules. The automated detection device disclosed in Chinese utility model patent CN203490229U, published on March 19, 2014, eliminates the mechanical gripper design and introduces a pulley drive mechanism into the automated detection module. This mechanism drives the reaction vessel to shuttle between different operating positions to perform the entire detection process. This approach requires very high precision in layout and transmission, resulting in significant limitations in application. All of these types of automated enzyme immunoassay systems are constrained by factors such as cost, reliability, and control precision, necessitating a more systematic and optimized design for automated enzyme immunoassay systems.

[0004] The enzyme immunoassay (ELISA) equipment needs further optimization in terms of spatial layout, cost savings, and efficient and reliable configuration for pipetting or consumable transfer. Designing more reliable and efficient ELISA equipment remains an urgent technical problem to be solved. Utility Model Content

[0005] To address the aforementioned problems in the existing technology, this utility model is achieved through the following technical solution:

[0006] This invention provides an automated enzyme-linked immunosorbent assay (ELISA) device, including a processing module and a washing module. The processing module includes a pipetting module, a gripper module, and an operation processing area. The operation processing area is provided with a reaction container, a washing position, and a washing head that cooperates with the washing position. The washing head includes an injection needle and an aspiration needle. Both the pipetting module and the gripper module can be driven to move along the extension direction of the operation processing area. The pipetting module can move to the reaction container and transfer sample solution or reagents to the reaction container. The gripper module can move to the reaction container to open and close the reaction container and transfer the reaction container to the washing position. The washing module is fluidly connected with the injection needle and the aspiration needle to clean the reaction container transferred to the washing position.

[0007] In one embodiment of this utility model, the processing module and the washing liquid module are structurally independent.

[0008] In one embodiment of this utility model, the washing liquid module includes a rinsing tank, a washing tank group formed by multiple washing tanks, a waste liquid collection tank, a filling pump, a control valve group, and a vacuum pump. The waste liquid collection tank is fluidly connected to a suction needle. The filling pump is connected to either the rinsing tank or one of the washing tanks through the control valve group. The filling pump is also fluidly connected to a filling needle. The washing position is also provided with a suction port. Both the suction needle and the suction port are fluidly connected to the waste liquid collection tank. The waste liquid collection tank is connected to a vacuum pump, which can draw water from the waste liquid collection tank to create a negative pressure state in the waste liquid collection tank.

[0009] In one embodiment of this utility model, the washing liquid module is provided with a central partition, and there are multiple filling pumps, with at least some of the filling pumps connected to the central partition.

[0010] In one embodiment of this utility model, an oscillation incubation area is further configured in the extension direction of the operation processing area. A metal-based printed circuit board is configured in the oscillation incubation area as a heat source. The oscillation incubation area is also provided with multiple oscillation incubation units. The reaction vessel can be configured in each oscillation incubation unit. The metal-based printed circuit board includes a metal substrate layer. A heating surface and a printing surface are provided on the substrate layer. The heating surface and the printing surface are arranged opposite to each other. The heating surface can make thermal contact with the reaction vessel in the constant temperature incubation reaction. An insulating surface is connected to the printing surface. A metal heating layer is printed on the insulating surface. When the metal heating layer is electrically heated, heat is uniformly transferred through the substrate layer to the reaction vessel in thermal contact with the heating surface.

[0011] In one embodiment of this utility model, an auxiliary functional area is further configured in the extending direction of the operation processing area. The auxiliary functional area is adjacent to the oscillation incubation area. The auxiliary functional area includes multiple incubation cover storage areas, each consisting of multiple incubation cover storage positions. The gripper module can move between the reaction vessel and the auxiliary functional area and can grip the incubation cover to perform opening and closing operations on the reaction vessel.

[0012] In one embodiment of this utility model, the operation processing area is further provided with a sample loading area, a consumable loading area, and a reagent loading area in sequence along its extension direction. The pipetting module can move to the sample loading area, the consumable loading area, and the reagent loading area respectively to realize sample solution transfer, consumable loading, and reagent transfer. The sample loading area is provided with multiple parallel sample loading channels, each of which can receive one sample tube rack. The consumable loading area can hold a whole plate of pipetting consumables. The reagent loading area is provided with a guide rail, which is detachably connected to a reagent loading rack. The reagent loading rack is provided with multiple reagent loading positions, and multiple reagent loading positions can insert multiple reagent baskets.

[0013] In one embodiment of this utility model, a plate washing area and an enzyme immunoassay area are also included. The plate washing area includes a plate washing assembly, which is provided with the cleaning head. The cleaning head is movably fitted to the reaction container within the cleaning position to perform cleaning of all reaction wells of the reaction container within the cleaning position.

[0014] In one embodiment of the present invention, a common drive mechanism is further included. The common drive mechanism includes a crossbeam disposed on the top of the processing module. A common slide rail extending along the length direction is connected to the side of the crossbeam. A first transverse connecting block and a second transverse connecting block are connected to the common slide rail. The first transverse connecting block is connected to the pipetting module, and the second transverse connecting block is connected to the gripper module. The crossbeam is disposed at a position slightly off-center from the top of the pipetting module and the gripper module.

[0015] In one embodiment of this utility model, a common guide rod is also arranged on the processing module, and both the pipetting module and the gripper module are provided with roller sets. The pipetting module and the gripper module are respectively connected to the common guide rod in a rolling manner through the corresponding roller sets. The pipetting module and the gripper module are also respectively configured with a transmission mechanism. The transmission mechanism includes a drive motor, a drive wheel, a tension wheel, and a transmission belt fixed to the top of the processing module. The output shaft of the drive motor is connected to the drive wheel, and the drive wheel is pre-tightly wound with the transmission belt in cooperation with the tension wheel.

[0016] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0017] 1. This utility model provides an automated enzyme-linked immunosorbent assay (ELISA) device. Based on the concept of segmentation, the device is divided into a processing module and a washing module, which is structurally independent of the processing module. The configuration positions of the two modules are flexible and variable, allowing the device to adapt to different spatial requirements. The processing module includes a pipetting module and a gripper module. The pipetting module is equipped with multiple pipetting units to efficiently perform sample or reagent transfer. A crossbeam at the top of the processing module connects the pipetting module and the gripper module respectively through two transverse connecting blocks. The two modules are also connected to a common guide rod to ensure reliable operation of both modules in the direction of the operating processing area. Furthermore, the crossbeam is positioned slightly off-center at the top of the processing module, and the distance between the crossbeam and the common guide rod is less than the length of the pipetting module and / or the gripper module. This allows for good adaptation to the heavy loads of the pipetting module composed of multiple pipetting units, better balancing the gravity transmitted by the corresponding transverse connecting blocks during pipetting operations, and reducing the reliability risks such as deformation and accelerated wear of the connection between the connecting blocks and the crossbeam under high loads.

[0018] 2. The automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model features a transmission mechanism in which the transverse drive motors for the pipetting module and the gripper module are configured to follow the corresponding modules. This maximizes the operating stroke of the pipetting module and the gripper module. By using two independent and fixed transverse transmission belts in conjunction with the drive wheels driven by the corresponding drive motors, the risk of interference during the operation of the pipetting module and the gripper module is minimized, ensuring the long-term reliability of both modules. Furthermore, the two transmission belts are configured with essentially the same length, which improves maintainability. The fixed ends of the transmission belts simplify the configuration of the transmission mechanism. Compared to the method of winding the transmission belts, the risk of breakage is lower, making it more suitable for applications in long-stroke scenarios.

[0019] 3. In the automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model, the multiple arrayed oscillation incubation units configured in the oscillation incubation zone can perform efficient incubation reactions for multiple items or samples in parallel. The oscillation incubation zone includes a metal-based printed circuit board as a heat source, which can achieve efficient direct heating and has higher long-term operational reliability. An incubation cap storage area is also configured adjacent to the oscillation incubation zone, which can cooperate with the oscillation incubation unit to perform incubation reactions with the caps fastened. The washing module is equipped with a rinsing tank and multiple washing tanks. The filling pump can be connected to the rinsing tank or one of the washing tanks through a control valve group. The filling pump is also connected to the injection needle of the washing head. The injection needle adds washing solution to the ELISA reaction container in the washing position. The aspiration needle aspirates the added washing solution waste. Through several filling and aspiration actions, the ELISA reaction container is automatically cleaned. At the same time, the washing position also includes a suction port connected to the waste liquid collection tank, so that even if there is too much washing solution and possible overflow, it can be promptly and effectively aspirated. By configuring a rinsing station that is not connected to the washing station, a temporary storage location can be provided for the washing head, and the rinsing and cleaning operations of the washing head can be performed intermittently without interference, avoiding the risk of clogging. Multiple washing tanks can, in one scenario, increase the amount of washing solution stored in the washing module to ensure the continuous operation time of the washing module; in another scenario, by configuring different types of washing solutions for different washing tanks, compatibility with enzyme immunoassay reaction containers from different manufacturers can be achieved, improving the compatibility and versatility of the equipment in the enzyme immunoassay cleaning system. Furthermore, the number of washing heads can be configured to one or more. When multiple washing heads are used, the cleaning efficiency is improved, making the enzyme immunoassay detection equipment operate more efficiently. Reaction containers undergoing incubation in the shaking incubation zone can be transferred to the enzyme immunoassay detection zone set up in the processing module after being cleaned by the washing module. This achieves a fully automated enzyme immunoassay detection process, including sample transfer, reagent transfer, enzyme immunoassay reaction, cleaning, and enzyme immunoassay detection, reducing the risk of contamination in the enzyme immunoassay reaction and improving incubation efficiency and accuracy.

[0020] 4. The automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model has multiple arrayed oscillation incubation units configured in the oscillation incubation area, which can perform efficient incubation reactions for multiple items or multiple samples in parallel. At the same time, the oscillation incubation area is equipped with a metal-based printed circuit board as a heat source, which can achieve a direct heating effect and higher long-term reliability. An incubation cover storage area is also configured adjacent to the oscillation incubation area. The incubation cover storage area can work with the gripper module to perform an incubation reaction of locking the cover of the oscillation incubation unit, which makes the incubation efficiency and accuracy higher.

[0021] 5. The automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model, by reasonably setting the positions of different functional areas on the operation and processing area, enables different functional areas to be optimally adapted to the pipetting module and the gripper module for functional operation, thereby reducing the risk of detection contamination, and at the same time, the small distance between the two makes the transfer more reliable.

[0022] 6. The automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model has the liquid pump and vacuum pump in the washing module configured on different assembly plates, which reduces the risk of resonance during operation of the washing module and makes the entire washing module structure more compact and the layout more reasonable.

[0023] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the overall structure of an automated enzyme-linked immunosorbent assay (ELISA) device provided in an embodiment of this utility model;

[0025] Figure 2 This is a schematic diagram of the overall structure of another automated enzyme-linked immunosorbent assay (ELISA) device provided in this embodiment of the present invention;

[0026] Figure 3 This is a schematic diagram of the structure of the extractable reagent loading rack provided in this embodiment of the utility model;

[0027] Figure 4 This is a schematic diagram of the inner structure of the drive structure in the pipetting module and gripper module of the automated enzyme-linked immunosorbent assay (ELISA) device provided in this embodiment of the present invention;

[0028] Figure 5 This is a schematic diagram of the overall structure of the drive structure in the pipetting module and gripper module provided in this embodiment of the utility model;

[0029] Figure 6 This is one of the schematic diagrams showing the process of a pipetting module driven to complete a sample transfer according to an embodiment of the present invention;

[0030] Figure 7 This is a second schematic diagram of the sample transfer process completed by the pipetting module in this embodiment of the present invention;

[0031] Figure 8 This is the third schematic diagram of the sample transfer process being completed by the pipetting module provided in this embodiment of the present invention;

[0032] Figure 9 This is one of the schematic diagrams showing the process of the oscillation incubation unit being covered after the sample loading is completed in the automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model;

[0033] Figure 10 This is the second schematic diagram of the process of the oscillation incubation unit being covered after the sample loading is completed in the automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model;

[0034] Figure 11This is one of the schematic diagrams of the reaction vessel being loaded into the cleaning bitmap after completing the oscillation incubation in the automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model;

[0035] Figure 12 This is the second schematic diagram of the automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model, showing the reaction vessel being loaded into the cleaning bitmap after completing the shaking incubation.

[0036] Figure 13 This is one of the structural schematic diagrams of the washing solution module that performs reaction container cleaning in conjunction with the cleaning station in the automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model;

[0037] Figure 14 This is the second schematic diagram of the washing solution module that performs reaction container cleaning in conjunction with the cleaning station in the automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model.

[0038] Figure 15 This is a schematic diagram showing the state of the automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model after cleaning, where the gripper module transfers the reaction container to the ELISA detection area.

[0039] Explanation of reference numerals in the attached figures:

[0040] 10-Processing module;

[0041] 11-Pipeline module; 110-Pipeline unit; 1100-Crossbeam; 1101-Step-type sensing module; 111-Pipeline transverse drive motor; 112-First assembly block; 113-First transverse transmission belt; 114-First transverse connecting block; 115-Pipeline roller assembly;

[0042] 12-Gripper module; 1200-Common guide rod; 120-Mechanical gripper; 121-Gripper transverse drive motor; 122-Second assembly block; 123-Second transverse transmission belt; 124-Second transverse connecting block; 125-Gripper roller assembly;

[0043] 13-Sample application area; 131-Sample tube rack; 1300-Scanning unit;

[0044] 14 - Consumable loading area; 1400 - Vision sensing unit;

[0045] 15 - Reagent loading area; 151 - Reagent loading position;

[0046] 16 - Oscillation incubation zone; 161 - Oscillation incubation unit;

[0047] 17-Auxiliary function area; 171-Incubation cover storage area; 172-Self-test area;

[0048] 18 - Washing area; 181 - Washing head;

[0049] 19-Enzyme immunoassay area;

[0050] 20-Washing liquid module; 21-Liquid tank support position; 211-Sub-support position; 22-; 221-Sub-support position; 222-Liquid pump unit; 223-Control valve assembly; 224-Vacuum pump; 225-Waste liquid collection tank;

[0051] 300-washing solution tank;

[0052] 400-Tube-type reagent bottle. Detailed Implementation

[0053] To further illustrate the technical means and effects adopted by this utility model to achieve its intended purpose, the following detailed description of the solution based on this utility model is provided in conjunction with the accompanying drawings and specific embodiments.

[0054] The foregoing and other technical contents, features, and effects of this utility model will be clearly presented in the following detailed description of the specific embodiments with reference to the accompanying drawings. Through the description of the specific embodiments, a more in-depth and specific understanding can be gained of the technical means and effects adopted by this utility model to achieve the intended purpose. However, the accompanying drawings are only provided for reference and illustration and are not intended to limit the technical solution of this utility model.

[0055] It should be noted that in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. The term "and / or" is used to describe the relationship between related objects, for example, indicating that there can be three relationships between related objects. For example, A and / or B can represent: A alone, A and B simultaneously, and B alone. In this document, the character " / " generally indicates that the related objects are in an "or" relationship. The terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product of this utility model is in use. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Unless otherwise explicitly specified and limited, the terms “set up,” “install,” “connect,” and “link” should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection between two components.

[0056] Please see Figure 1 and Figure 2 , Figure 1 This is a schematic diagram of the overall structure of an automated enzyme-linked immunosorbent assay (ELISA) device provided in an embodiment of this utility model. Figure 2 This is a schematic diagram of the overall structure of another automated enzyme-linked immunosorbent assay (ELISA) device provided in this embodiment. The automated ELISA device includes two modules: a processing module 10 and a washing solution module 20, which is structurally independent of the processing module 10. A fluid pipeline can be configured between the two modules to allow for the addition of washing solution to the processing module 10 and the recycling of waste solution. The automated ELISA device provided in this embodiment integrates the washing solution-related functional units, configuring them independently as integrated functional modules based on a segmented design approach. During client configuration, the washing solution module 20 can be positioned in any of the six directions (front, back, left, right, up, down) of the processing module 10. The relatively independent structures of the processing module 10 and the washing solution module 20 allow for multiple relative orientations, making the installation environment requirements for this automated ELISA device very low. The integrated configuration of the two modules also simplifies and speeds up assembly at different relative positions.

[0057] This automated enzyme-linked immunosorbent assay (ELISA) device includes a processing module 10 and a washing module 20. The processing module 10 includes a pipetting module 11, a gripper module 12, and an operating area. The operating area includes a reaction container, a washing position, and a washing head 181 that cooperates with the washing position. The washing head 181 includes an injection needle and an aspiration needle. Both the pipetting module 11 and the gripper module 12 can be driven to move along the extension direction of the operating area. The pipetting module 11 can move to the reaction container and transfer sample solution or reagents into it. The gripper module 12 can move to the reaction container to open and close the container and transfer it to the washing position. The washing module 20 is fluidly connected to the injection needle and the aspiration needle to wash the reaction container transferred to the washing position. For example, when the gripper module 12 transfers the reaction container to the washing position, the washing module 20 can fluidly cooperate with the washing head 181 to complete the washing of the reaction container.

[0058] Furthermore, the processing module 10 includes a bottom substrate, on which different functional operation processing sub-regions are configured to form an operation processing area. This operation processing area includes sequentially adjacent sample loading area 13, consumable loading area 14, reagent loading area 15, shaking incubation area 16, and auxiliary function area 17. The operation processing area also includes a consumable unloading area, a plate washing area 18, and an enzyme immunoassay area 19. The operation processing area also includes a washing position, and a washing head 181 is provided in the plate washing area 18 in conjunction with the washing position. The shaking incubation area 16 houses the reaction container. A pipetting module 11 and a gripper module 12 are located in the upper region of the processing module 10. The pipetting module 11 can move to the sample loading area 13, consumable loading area 14, and reagent loading area 15 respectively to perform sample transfer, consumable loading, and reagent transfer operations. The gripper module 12 can move between the reaction container and the auxiliary function area 17 and can hold the incubation cap to open and close the reaction container. The pipetting module 11 and the gripper module 12 can be driven to perform pipetting or gripping operations between different positions in the operating processing area of ​​the processing module 10. The processing module 10 may include a housing and a control unit, the housing enclosing the operating processing area and the pipetting module 11 and gripper module 12 located above the operating processing area.

[0059] Furthermore, the sample loading area 13 is equipped with multiple parallel sample loading channels, each capable of receiving one sample tube rack 131. This arrangement allows for the loading of dozens or nearly a hundred sample tubes at once in the sample loading area 13. To ensure ease of subsequent pipetting, all sample tubes in the sample rack can be kept in the open position. Furthermore, each sample loading channel is equipped with a sensing unit, and the processing module 10 is also equipped with a barcode scanning unit 1300 to cooperate with the sample loading area 13. The barcode scanning unit 1300 can slide along a direction perpendicular to the insertion of the sample tube rack into the sample loading area 13, enabling precise barcode scanning of the loaded sample tube rack and / or sample tubes. This achieves the design goal of timely and effective identification of the loaded sample tube rack in the sample loading channel.

[0060] The consumable loading area 14 is adjacent to the sample loading area 13. The consumable loading area 14 can hold a full plate of pipetting consumables, and the pipetting module 11 can move to the consumable loading area 14 to load the pipetting consumables. The consumables can be transparent or opaque pipetting tips. A full plate of pipetting tips can be loaded, with each plate holding 96 individual pipetting tips. Using disposable pipetting tips reduces or eliminates the risk of contamination during sample or reagent transfer. The consumable loading area 14 is equipped with a consumable receiving rack of preset height, with receiving positions that can accommodate multiple full plates of pipetting tips to ensure a sufficient number of pipetting tips are loaded in a single operation. The receiving positions provided in this embodiment ensure reliable and efficient storage of the pipetting tips.

[0061] The reagent loading area 15 is adjacent to the consumable loading area 14. The reagent loading area 15 is provided with a guide rail, which is detachably connected to the reagent kit loading rack. Preferably, the guide rail is removable and detachably connected to the reagent kit loading rack. Figure 3 As shown, the reagent loading rack can be slidably connected to the reagent loading area 15. The reagent loading rack is also equipped with two or more reagent loading positions 151. The two or more reagent loading positions 151 can insert multiple identical or multiple different reagent baskets. The reagent baskets can load reagents. The cylindrical tubular reagent bottle 400 can be compatible with loading different sizes of this type of reagent bottle through the optimized design of the support block, so as to realize batch and efficient reagent loading operation.

[0062] The oscillating incubation zone 16 is equipped with a metal-based printed circuit board as a heat source. The oscillating incubation zone 16 also contains multiple oscillating incubation units 161, specifically, multiple oscillating incubation units 161 are arranged in an array within the oscillating incubation zone 16. Each oscillating incubation unit 161 can be equipped with a reaction vessel. The metal-based printed circuit board includes a metal substrate layer, on which a heating surface and a printing surface are disposed. The heating surface and the printing surface are positioned opposite each other. The heating surface can make thermal contact with the reaction vessel in the constant-temperature incubation reaction. The printing surface is connected to an insulating surface, and a metal heating layer is printed on the insulating surface. When the metal heating layer is electrically heated, heat is uniformly transferred through the substrate layer to the reaction vessel in thermal contact with the heating surface, thereby achieving parallel and efficient incubation reaction operation.

[0063] Furthermore, the heating layer is divided into multiple heating regions with different average power densities, and the heating layer is configured such that the average power density of the central region is lower than that of the edge region.

[0064] Specifically, the metal-based printed circuit board includes a metal substrate layer, and the substrate layer includes a printed surface with a metal heating layer. The heating layer is configured such that the average power density in the central region is lower than that in the edge region, resulting in a distribution trend where the average power density of the heating layer on the directly heated printed circuit board increases from the center to the edge. This ensures the uniformity of the output temperature of the heating layer on the directly heated printed circuit board. The average power density in the central region is configured to be within the range of 0.54-0.83 times that of the average power density in the edge region, directly adjusting the difference in average power density between the central and edge regions at the heat source end. The automated enzyme-linked immunosorbent assay (ELISA) device provided in this embodiment uses a metal-based printed circuit board as a heating source, and the heating layer is printed on one side of the metal substrate in the thickness direction. This results in lower thermal resistance and adaptability to oscillating motion scenarios, avoiding a decrease in the heat transfer coefficient due to reliability issues.

[0065] An auxiliary functional area 17 is also arranged at the edge of the operating table, adjacent to the oscillation incubation area 16. The auxiliary functional area 17 includes multiple incubation cap storage areas 171, each consisting of multiple incubation cap storage positions capable of storing incubation caps. The auxiliary functional area 17 also includes a self-testing area 172 for cooperative inspection. The gripper module 12 is movable between the reaction vessel and the auxiliary functional area 17 and is capable of gripping the incubation caps to perform opening and closing operations on the reaction vessel.

[0066] Furthermore, a plate washing area 18 and an enzyme immunoassay (ELISA) detection area 19 are arranged side-by-side within the processing module 10. The plate washing area 18 includes two or more washing components to efficiently clean the reaction vessel. The side-by-side arrangement of the plate washing area 18 and the ELISA detection area 19 within the processing module 10 ensures that operations such as reaction vessel cleaning and ELISA detection do not interfere with the operation steps of the sample loading area 13, consumable loading area 14, reagent loading area 15, shaking incubation area 16, and auxiliary function area 17, which are used in conjunction with the ELISA reaction in the processing area. Simultaneously, the gripper transfer path is shorter. The plate washing components are equipped with a washing head 181, which is movably fitted to the reaction vessel within the cleaning position to clean all reaction wells of the reaction vessel within the cleaning position. In this embodiment, the plate washing area 18 and the ELISA detection area 19 are arranged adjacently in the inner region of the device, reducing the risk of detection contamination, and the smaller distance between them ensures higher reliability of the transfer operation.

[0067] Please refer to Figure 4 and Figure 5 , Figure 4 This is a schematic diagram of the inner structure of the drive structure in the pipetting module and gripper module of the automated enzyme-linked immunosorbent assay (ELISA) device provided in this embodiment of the present invention. Figure 5This is a schematic diagram of the overall structure of the driving structure in the pipetting module and gripper module provided in this embodiment of the utility model. The pipetting module 11 includes four or more pipetting units 110 to achieve more efficient pipetting operations. Each pipetting unit 110 can be an independently driven pipetting ADP. This arrangement ensures sufficient and accurate dispensing of reagents or samples in each reaction well. Optimally, the pipetting module 11 can contain eight pipetting units 110 to efficiently dispensing reagents into the eight reaction wells in the reaction container, reducing the frequency of back-and-forth driving of the pipetting module, improving dispensing efficiency, and enhancing the reliability of module operation. To coordinate the precise position control of multiple pipetting units 110, the pipetting module 11 is also equipped with a stepped sensing module 1101. Each pipetting unit is also linked to a sensor. At least a portion of the steps of the stepped sensing module 1101 can be coupled to the sensors included in the pipetting module 11 to accurately position or limit each pipetting unit. This arrangement ensures that each pipetting unit in the pipetting module 11 can always be driven efficiently and accurately. The gripper module 12 is equipped with mechanical grippers 120 capable of clamping, such as... Figure 2 As shown, the gripper module 12 can also be equipped with a vision sensing unit 1400 that cooperates with the mechanical gripper 120. During at least a portion of the travel, the vision sensing unit 1400 can follow the mechanical gripper 120 (here illustrated as following in four horizontal dimensions: front, back, left, and right). This allows for precise guidance of the mechanical gripper 120's movement and real-time monitoring of the status of the transferred reaction container. In enzyme immunoassay scenarios, the most frequently involved operations are the transfer of sample solutions, reagents, etc., and the transfer of reaction containers.

[0068] Ideally, independent drives are configured for the pipetting module 11 and the gripper module 12 to ensure reliable operation of different operations such as sample transfer, reagent transfer, and reaction vessel transfer.

[0069] Furthermore, the automated enzyme-linked immunosorbent assay (ELISA) device also includes a common drive mechanism, which includes a crossbeam 1100 disposed on the top of the processing module 10. A common slide rail extending along the length direction is connected to the side of the crossbeam 1100. A first transverse connecting block 114 and a second transverse connecting block 124 are connected to the common slide rail. The first transverse connecting block 114 is connected to the pipetting module 11, and the second transverse connecting block 124 is connected to the gripper module 12. The crossbeam 1100 is disposed at a position slightly above the center of the top of the pipetting module 11 and the gripper module 12.

[0070] Furthermore, a common guide rod 1200 is arranged on the processing module 10. Both the pipetting module 11 and the gripper module 12 are equipped with roller sets, and the pipetting module 11 and the gripper module 12 are respectively connected to the common guide rod 1200 through the corresponding roller sets. The pipetting roller set 115 and the gripper roller set 125 can contain different numbers of rollers to reliably support functional modules of different weights, while also reducing the running resistance of different functional modules. The pipetting module 11 and the gripper module 12 are also respectively equipped with a transmission mechanism. The transmission mechanism includes a drive motor, a drive wheel, a tension wheel, and a transmission belt fixed to the top of the processing module 10. The output shaft of the drive motor is connected to the drive wheel, and the drive wheel is pre-tightly wound with the transmission belt in cooperation with the tension wheel.

[0071] In this embodiment, the crossbeam 1100 is positioned at the top center of the pipetting module 11 and the gripper module 12 to prevent deformation and wear of the connecting block and the crossbeam under high loads caused by the two modules being connected to the crossbeam 1100 at their ends. In the optimal case, the distance between the crossbeam 1100 and the common guide rod 1200 is less than the length of the pipetting module 11 and / or the gripper module 12, thus enabling it to be well adapted to the heavy-duty scenarios of pipetting units composed of multiple pipetting units. This allows the gravity transmitted by the transverse connecting block corresponding to the pipetting module 11 and the gripper module 12 to be better balanced during pipetting operations, reducing the reliability risks such as deformation and accelerated wear of the connection between the connecting block and the crossbeam under high loads. The transmission mechanisms configured for the pipetting module 11 and the gripper module 12 are as follows: The top of the pipetting module 11 is equipped with a pipetting lateral drive motor 111. The output shaft of the pipetting lateral drive motor 111 is connected to a pipetting lateral drive wheel. The pipetting lateral drive wheel is fitted with a first lateral transmission belt 113 fixedly arranged on the top of the processing module 10. The pipetting lateral drive wheel can be pre-tensioned with the first lateral transmission belt 113 using a tensioning wheel. Here, to ensure a reliable connection between the pipetting lateral drive motor 111 and the pipetting module 11, the motor is mounted on a first assembly block 112. Similarly, the top of the gripper module 12 is equipped with a gripper lateral drive motor 121. The output shaft of the gripper lateral drive motor 121 is connected to a gripper lateral drive wheel. The gripper lateral drive wheel is fitted with a second lateral transmission belt 123 fixedly arranged on the top of the processing module 10. The gripper lateral drive wheel and the second lateral transmission belt 123 can also have their pre-tensioning force adjusted by a tensioning wheel. The gripper lateral drive motor 121 is mounted on a second assembly block 122. The first transverse transmission belt 113 and the second transverse transmission belt 123 have essentially the same length, resulting in better assembly and maintenance performance of the transmission mechanism. To ensure transmission reliability, both ends of the first transverse transmission belt 113 and the second transverse transmission belt 123 are fixed. With this configuration, when the pipetting transverse drive motor 111 or the gripper transverse drive motor 121 rotates, the fixed ends of the transmission belt cause the drive wheel to be driven to roll on the transmission belt. This is then driven by the pipetting transverse drive motor 111 and the gripper transverse drive motor 121, and moves with the pipetting module 11 or the gripper module 12 to shuttle between different operating areas of the processing module 10. Furthermore, compared to the moving transmission belt in the wheel-type transmission design, the fixed ends of the transmission belt, which drive the drive wheel to roll on the transmission belt, eliminate the need for an excessively long transmission belt, resulting in lower risk of transmission belt wear and breakage, and higher transmission accuracy.

[0072] Please refer to Figures 6-8 , Figures 6-8This is a schematic diagram illustrating the process of transferring sample solution by the pipetting module provided in this embodiment of the present invention. In this embodiment, multiple sample tube racks 131 are inserted into the sample loading area 13, and the sample tube racks 131 carry sample tubes with the tubes open. The sample loading area 13 scans the sample tubes with the assistance of the barcode scanning unit 1300. The consumable loading area 14 is equipped with several full-plate pipetting consumables, and the reagent loading area 15 is pre-configured with reagent kits.

[0073] The process of transferring sample solution by the pipetting module includes the following steps: First, the pipetting module 11 within the processing module 10 is driven to the consumable loading area 14 to load the pipetting tip. Then, the pipetting module 11 is driven to the sample application area 13, where the pipetting tip aspirates sample solution from the sample tube. After sample aspiration, the pipetting module 11 is driven to the shaking incubation area 16, where it adds sample solution to the reaction container via the pipetting tip. Of course, before adding sample solution, different types of reagents can be added to the reaction container via the pipetting module 11 to prevent cross-contamination. Adding different types of reagents to the reaction container via the pipetting module 11 is similar to the process described above where the pipetting module 11 is driven to add sample solution to the reaction container, and will not be elaborated further here. Finally, the pipetting module 11, having completed loading the sample solution and reagents, can be driven to the consumable unloading area to unload the pipetting tip. The recycling port of the consumable unloading area is rectangular and located at the front of the processing module 10, making consumable unloading and delivery easier.

[0074] Please refer to Figure 9 and Figure 10 , Figure 9 This is one of the schematic diagrams illustrating the process of the oscillation incubation unit being covered after sample loading in the automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model. Figure 10 This is the second schematic diagram of the process of closing the shaking incubation unit after sample loading in the automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model. Reagents and sample solutions can be added to the reaction containers in each shaking incubation unit 161 within the shaking incubation area 16 one by one. In this embodiment, the auxiliary functional area 17 includes a self-test area 172 and three incubation cap storage areas 171. After reagent and sample solution addition, the gripper module 12 can be driven to transfer the incubation cap from the incubation cap storage area 171 and fasten it onto the shaking incubation unit 161, allowing the ELISA reaction to be shaken and incubated in a closed state with the cap on. This ensures reaction efficiency while reducing the risk of contamination during the reaction. Since multiple shaking incubation units 161 are arrayed within the shaking incubation area 16, these units can operate gradually rather than simultaneously, thus fully utilizing the shaking incubation reaction time pattern to form a gradient of cleaning and detection, ensuring the efficient operation of the processing module 10. The choice of a metal-based printed circuit board as the heat source is also more suitable for the special operating scenario of the shaking incubation reaction.

[0075] Please refer to Figure 11 and Figure 12 , Figure 11 This is one of the schematic diagrams showing the reaction vessel of the automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model being loaded onto the cleaning bitmap after completing the shaking incubation. Figure 12 This is the second schematic diagram of the automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model, showing the reaction container after oscillation incubation being loaded into the cleaning position. After the automated ELISA device completes the oscillation incubation reaction, the gripper module 12 can be driven to remove the incubation cap from the oscillation incubation unit 161 to the incubation cap storage area 171. Then, the reaction container in the oscillation incubation unit 161 can be gripped by the mechanical gripper 120 and transferred to the cleaning position. To ensure the high efficiency of cleaning, the processing module 10 is equipped with no less than two cleaning positions. After the reaction container that has completed incubation is transferred into the cleaning position, the cleaning head 181 is connected to the washing solution module 20. The washing solution module 20 injects the cleaning solution into the reaction container. After cleaning is completed, the waste liquid is discharged through the cleaning head 181 into the waste liquid collection tank 225.

[0076] Please refer to Figure 13 and Figure 14 , Figure 13 This is one of the structural schematic diagrams of the washing solution module that performs reaction vessel cleaning in conjunction with the washing station in the automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model. Figure 14 This is the second schematic diagram of the washing solution module that performs reaction vessel cleaning in conjunction with the cleaning station in the automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model.

[0077] The washing liquid module 20 includes a rinsing tank and a washing tank group formed by multiple washing tanks. Here, the washing tank and the rinsing tank can be configured as washing liquid tanks 300 with the same external dimensions to achieve the goal of low-cost configuration. The washing liquid module 20 also includes a waste liquid collection tank 225, a filling pump, a control valve group 223, and a vacuum pump 224. The waste liquid collection tank 225 can be fluidly connected to a suction needle. The filling pump can be connected to the rinsing tank or one of the washing tanks through the control valve group 223. The filling pump can also be fluidly connected to the injection needle. The washing position is also provided with a suction port. Both the suction needle and the suction port can be fluidly connected to the waste liquid collection tank 225. The waste liquid collection tank 225 is connected to the vacuum pump 224. The vacuum pump 224 can draw the waste liquid collection tank 225 to make the waste liquid collection tank 225 a negative pressure state.

[0078] Preferably, the length of the aspiration needle exposed outside the cleaning head base is greater than the length of the injection needle exposed outside the cleaning head base. Most preferably, the length of the injection needle exposed outside the cleaning head base is less than half the length of the aspiration needle exposed outside the cleaning head base. The ratio of the length of the injection needle exposed outside the cleaning head base to the length of the aspiration needle exposed outside the cleaning head base can also be 1 / 3, 1 / 4, 2 / 5, 1 / 5, or approximately these ratios. When the aspiration needle aspirates the used waste liquid, even if there is a certain deviation in the position driven by the lifting mechanism, the significant height difference between the lengths of the injection needle and the aspiration needle exposed outside the cleaning head base greatly reduces the risk of the injection needle contacting the liquid during pipetting, thus reducing the risk of contamination during injection and waste liquid aspiration and discharge.

[0079] Furthermore, the washing liquid module 20 also includes a waste liquid pump, one end of which is connected to the waste liquid collection tank 225, and the other end is connected to the waste liquid storage tank (not shown in the figure).

[0080] The washing liquid module 20 is connected to the cleaning head 181 via a connecting pipe. The washing liquid module 20 includes multiple liquid tank bearing positions 21 configured on the common washing liquid base plate. Each liquid tank bearing position 21 includes multiple sub-bearing positions 211, which can hold different types of cleaning liquid or washing liquid. In order to ensure the accuracy of liquid filling, each sub-bearing position 211 is equipped with a weight sensor. A vacuum pump 224 is also connected to the common washing liquid base plate. Specifically, the vacuum pump 224 is connected to the common washing liquid base plate via a vibration damping pad. The common washing liquid base plate is also fixedly connected to the waste liquid collection tank 225 and the control valve group 223 mounted on the control valve frame. The washing liquid module 20 is provided with a central partition in the middle. There are multiple filling pumps, and at least some of the filling pumps are connected to the central partition. Specifically, the central partition is fixedly connected to the central area of ​​the common washing liquid base plate. The central partition is located between the multiple liquid tank bearing positions 21 and other components. Other components refer to other parts on the washing liquid module 20 other than the multiple liquid tank bearing positions 21. Setting both the filling pump and the waste pump as the same type of liquid pump unit reduces costs. Multiple liquid pump units 222 are connected to the central partition, with multiple filling pumps and only one waste pump, resulting in higher operating efficiency and energy savings. To reduce operating noise and vibration of the liquid pump units 222, each unit is connected to the central partition via a vibration damping pad. The liquid pump units 222 and vacuum pump 224 within the washing module 20 are mounted on different assembly plates, reducing the risk of resonance during operation and making the entire washing module 20 more compact and rationally laid out. The central partition also provides more reliable support for the piping within the washing module 20 and isolates the impact of vibrations from the vacuum pump 224 and liquid pump units 222 on the weight sensor within the sub-bearing position 211. The cleaning head 181 is connected to the washing module 20 via a connecting pipe, adding cleaning fluid to the reaction vessel within the cleaning position, and then transferring the recovered waste liquid to the waste liquid collection tank 225. In this embodiment, the liquid tank bearing position 21 includes four sub-bearing positions 211, which can bear the washing liquid tank 300 and be configured with different types of cleaning liquid or washing liquid.

[0081] Please refer to Figure 15 , Figure 15 This is a schematic diagram of the state of the automated enzyme-linked immunosorbent assay (ELISA) device provided by this utility model after cleaning, in which the gripper module transfers the reaction container to the ELISA detection area. After the reaction container is cleaned, the gripper module 12 is driven to transfer the reaction container from the cleaning position to the ELISA detection area 19, and the gripped reaction container is placed into at least one ELISA detection area 19 arranged in the processing module 10 to complete the ELISA detection.

[0082] The automated enzyme-linked immunosorbent assay (ELISA) device provided in this embodiment includes a processing module with a pipetting module and a gripper module, and a relatively independent washing module. The configuration of the processing and washing modules is flexible and adaptable to different space requirements. A crossbeam at the top of the processing module connects the pipetting and gripper modules via two transverse connecting blocks. The pipetting and gripper modules are also connected to a common guide rod to ensure reliable operation. The transverse drive motors for both the pipetting and gripper modules are designed to follow the movement of their respective modules, maximizing the travel range of both functional modules. The pipetting and gripper modules are connected to drive wheels driven by their corresponding motors via two independent, fixed transverse transmission belts, minimizing interference risks during operation and ensuring long-term reliability.

[0083] In the automated enzyme-linked immunosorbent assay (ELISA) device provided in this embodiment, the multiple arrayed oscillation incubation units configured in the oscillation incubation zone can perform efficient incubation reactions for multiple projects or samples in parallel. The oscillation incubation zone includes a metal-based printed circuit board as a heat source, achieving efficient direct heating and higher long-term operational reliability. An incubation cap storage area is also configured adjacent to the oscillation incubation zone, which can cooperate with the oscillation incubation units to perform incubation reactions with the caps engaged. The washing module is equipped with a rinsing tank and multiple washing tanks. A filling pump can be connected to either the rinsing tank or one of the washing tanks via a control valve group. The filling pump is also connected to the injection needle of the washing head. The injection needle injects washing solution into the ELISA reaction container at the washing position, and the aspiration needle aspirates the injected washing solution waste. Through several injection and aspiration actions, the ELISA reaction container is automatically cleaned. The washing position also includes a suction port connected to a waste liquid collection tank, so that even if there is excessive washing solution, any potential overflow can be promptly and effectively aspirated. By configuring a rinsing station that is not connected to the washing station, a temporary storage location can be provided for the washing head, and the rinsing and cleaning operations of the washing head can be performed intermittently without interference, avoiding the risk of clogging. Multiple washing tanks can, in one scenario, increase the amount of washing solution stored in the washing module to ensure the continuous operation time of the washing module; in another scenario, by configuring different types of washing solutions for different washing tanks, compatibility with enzyme immunoassay reaction containers from different manufacturers can be achieved, improving the compatibility and versatility of the equipment in the enzyme immunoassay cleaning system. Furthermore, the number of washing heads can be configured to one or more. When multiple washing heads are used, the cleaning efficiency is improved, making the enzyme immunoassay detection equipment operate more efficiently. Reaction containers undergoing incubation in the shaking incubation zone can be transferred to the enzyme immunoassay detection zone set up in the processing module after being cleaned by the washing module. This achieves a fully automated process of sample transfer, reagent transfer, enzyme immunoassay reaction, cleaning, and enzyme immunoassay detection, reducing the risk of contamination in the enzyme immunoassay reaction and improving incubation efficiency and accuracy.

[0084] The above description, in conjunction with specific preferred embodiments, provides a further detailed explanation of the present invention. It should not be construed that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art, various simple deductions or substitutions can be made without departing from the concept of the present invention, and all such modifications and substitutions should be considered within the protection scope of the present invention.

Claims

1. An automated enzyme-linked immunosorbent assay (ELISA) device, characterized in that, The system includes a processing module (10) and a washing module (20). The processing module (10) includes a pipetting module (11), a gripper module (12), and an operation processing area. The operation processing area is provided with a reaction container, a washing position, and a washing head that cooperates with the washing position. The washing head includes an injection needle and an aspiration needle. Both the pipetting module (11) and the gripper module (12) can be driven to move along the extension direction of the operation processing area. The pipetting module (11) can move to the reaction container and transfer sample liquid or reagents to the reaction container. The gripper module (12) can move to the reaction container to open and close the reaction container and transfer the reaction container to the washing position. The washing module (20) can be fluidly connected with the injection needle and the aspiration needle to clean the reaction container transferred to the washing position.

2. The automated enzyme-linked immunosorbent assay (ELISA) device according to claim 1, characterized in that, The processing module (10) and the washing liquid module (20) are structurally independent.

3. The automated enzyme-linked immunosorbent assay (ELISA) device according to claim 1, characterized in that, The washing liquid module (20) includes a rinsing tank, a washing tank group formed by multiple washing tanks, a waste liquid collection tank, a filling pump, a control valve group, and a vacuum pump. The waste liquid collection tank can be fluidly connected to a suction needle. The filling pump can be connected to the rinsing tank or one of the washing tanks through the control valve group. The filling pump can also be fluidly connected to a filling needle. The washing position is also provided with a suction port. Both the suction needle and the suction port can be fluidly connected to the waste liquid collection tank. The waste liquid collection tank is connected to a vacuum pump. The vacuum pump can draw the waste liquid collection tank to make the waste liquid collection tank negative pressure.

4. The automated enzyme-linked immunosorbent assay (ELISA) device according to claim 3, characterized in that, The washing liquid module (20) is provided with a central partition, and there are multiple filling pumps, at least some of which are connected to the central partition.

5. The automated enzyme-linked immunosorbent assay (ELISA) device according to claim 1, characterized in that, The operation processing area is also extended by an oscillation incubation area (16), in which a metal-based printed circuit board is provided as a heat source. The oscillation incubation area (16) is also provided with multiple oscillation incubation units, and the reaction vessel can be configured in each oscillation incubation unit. The metal-based printed circuit board includes a metal substrate layer, on which a heating surface and a printing surface are provided. The heating surface and the printing surface are arranged opposite to each other. The heating surface can make thermal contact with the reaction vessel in the constant temperature incubation reaction. The printing surface is connected to an insulating surface, and a metal heating layer is printed on the insulating surface. When the metal heating layer is electrically heated, the heat is uniformly transferred through the substrate layer to the reaction vessel in thermal contact with the heating surface.

6. The automated enzyme-linked immunosorbent assay (ELISA) device according to claim 1, characterized in that, An auxiliary function area (17) is also provided in the direction of the operation processing area. The auxiliary function area (17) is adjacent to the oscillation incubation area (16). The auxiliary function area (17) includes multiple incubation cover storage areas, which are composed of multiple incubation cover storage positions. The gripper module (12) can move between the reaction vessel and the auxiliary function area (17) and can hold the incubation cover to perform opening and closing operations on the reaction vessel.

7. The automated enzyme-linked immunosorbent assay (ELISA) device according to claim 1, characterized in that, The operation processing area is further extended by a sample loading area (13), a consumable loading area (14), and a reagent loading area (15). The pipetting module (11) can move to the sample loading area (13), the consumable loading area (14), and the reagent loading area (15) respectively to realize sample solution transfer, consumable loading, and reagent transfer, respectively. The sample application area (13) is equipped with multiple parallel sample application channels, each of which can receive one sample tube rack; The consumable loading area (14) can support the entire plate of pipetting consumables; The reagent loading area (15) is provided with a guide rail, which is detachably connected to the reagent loading rack. The reagent loading rack is configured with multiple reagent loading positions, and multiple reagent loading positions can be used to insert multiple reagent baskets.

8. The automated enzyme-linked immunosorbent assay (ELISA) device according to claim 1, characterized in that, It also includes a plate washing area (18) and an enzyme immunoassay area (19). The plate washing area (18) includes a plate washing assembly, which is provided with the washing head. The washing head is movably fitted to the reaction vessel in the washing position to perform cleaning of all reaction wells of the reaction vessel in the washing position.

9. The automated enzyme-linked immunosorbent assay (ELISA) device according to claim 1, characterized in that, It also includes a common drive mechanism, which includes a crossbeam disposed on the top of the processing module (10), a common slide rail extending along its length connected to the side of the crossbeam, a first transverse connecting block and a second transverse connecting block connected to the common slide rail, the first transverse connecting block being connected to the pipetting module (11), the second transverse connecting block being connected to the gripper module (12), and the crossbeam being disposed at a position slightly off-center from the top of the pipetting module (11) and the gripper module (12).

10. The automated enzyme-linked immunosorbent assay (ELISA) device according to claim 9, characterized in that, The processing module (10) is also equipped with a common guide rod. The pipetting module (11) and the gripper module (12) are both equipped with roller sets. The pipetting module (11) and the gripper module (12) are respectively connected to the common guide rod by rolling through the corresponding roller sets. The pipetting module (11) and the gripper module (12) are also respectively equipped with a transmission mechanism. The transmission mechanism includes a drive motor, a drive wheel, a tension wheel and a transmission belt fixed to the top of the processing module (10). The output shaft of the drive motor is connected to the drive wheel. The drive wheel is pre-tightly wound with the transmission belt in cooperation with the tension wheel.

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