Extraction piece

Abstract

The extraction part is applied to the molecular diagnosis device, at least a reagent dispensing assembly and a sample dispensing assembly are arranged in a shell of the molecular diagnosis device, the extraction part comprises a first cavity and a second cavity which are open, the first cavity is used for containing a first reagent injected by the reagent dispensing assembly and a sample injected by the sample dispensing assembly, and the second cavity is used for containing a second reagent injected by the sample dispensing assembly. The second cavity is used for containing a second reagent injected by the reagent separate injection assembly. According to the extraction piece provided by the embodiment, the first cavity and the second cavity which are open are arranged, and the first reagent and the second reagent are injected into the first cavity and the second cavity through the molecular diagnosis device in the subsequent molecular diagnosis process, so that compared with an existing extraction piece in which the first reagent is preset in the first cavity and the second reagent is preset in the second cavity, the extraction piece provided by the embodiment has the advantages that the extraction efficiency is improved; and then the opening of the first cavity and the opening of the second cavity are sealed through the sealing film, and the extraction piece provided by the embodiment does not need to consider the problem of sealing film puncture in the subsequent molecular diagnosis process, so that the operation and the flow are simplified.

Description

[0001] The present application claims priority to the Chinese patent application No. 202311871110.0, filed on December 29, 2023, with the Chinese Patent Office, the whole content of which is incorporated herein by reference. TECHNICAL FIELD

[0002] The utility model relates to the field of medical apparatus and instruments, especially to an extraction piece. BACKGROUND

[0003] The extraction piece is a commonly used consumable in the field of molecular diagnostic technology, which is provided with multiple cavities for providing reaction space and containing reagents required in the molecular diagnostic process. In the prior art, the reagents required in the molecular diagnostic process are injected into the cavities of the extraction piece in advance, and the openings of the cavities are sealed by a sealing film. In the subsequent molecular diagnostic process, the sealing film on the extraction piece needs to be punctured one by one, which leads to low efficiency of the molecular diagnostic process. SUMMARY

[0004] Therefore, the utility model provides an extraction piece.

[0005] The extraction piece provided by the utility model in the first aspect is applied to a molecular diagnostic device, the shell of the molecular diagnostic device is provided with at least a reagent dispensing assembly and a sample dispensing assembly, the extraction piece comprises an open first cavity and a second cavity, the first cavity is used for containing a first reagent injected by the reagent dispensing assembly and a sample injected by the sample dispensing assembly, and the second cavity is used for containing a second reagent injected by the reagent dispensing assembly.

[0006] The extraction piece provided by the utility model in the second aspect comprises:

[0007] A strip-shaped piece comprises a first side and a second side opposite to the first side in the thickness direction of the strip-shaped piece, the strip-shaped piece is provided with at least two openings, the at least two openings are arranged along the length direction of the strip-shaped piece and penetrate through the strip-shaped piece in the thickness direction;

[0008] At least two tubes are arranged on the second side of the strip-shaped piece, and the at least two tubes and the at least two openings are in one-to-one correspondence and communication.

[0009] The strip-shaped piece comprises a first end face and a second end face opposite to the first end face in the length direction, the first end face is recessed to form a first positioning groove towards the second end face, and the second end face is recessed to form a second positioning groove towards the first end face.

[0010] From the above technical scheme can be seen, the utility model discloses a first aspect proposed extraction piece, through setting up having open first cavity and second cavity, and first reagent and second reagent are subsequent in the process of molecular diagnosis, and first cavity and second cavity are injected by molecular diagnostic device, relative to the first cavity of existing extraction piece and second cavity are preset with first reagent and second reagent, and then the opening of first cavity and the opening of second cavity are sealed by the mode of sealing membrane, the extraction piece proposed in this embodiment in the process of subsequent molecular diagnosis, do not need to consider the problem of sealing membrane puncture, can play the role of simplifying process. BRIEF DESCRIPTION OF DRAWINGS

[0011] In order to more clearly illustrate the technical scheme of the embodiments of the utility model, the drawings needed to be used in the embodiment description will be briefly introduced, and obviously, the drawings in the following description are some embodiments of the utility model, and for those skilled in the art, other drawings can be obtained without creative labor.

[0012] Figure 1 It is the structural schematic diagram of sample detection device that the utility model one embodiment proposes;

[0013] Figure 2 It is the structural schematic diagram of extraction assembly that the utility model one embodiment proposes;

[0014] Figure 3 It is the structural schematic diagram of extraction piece that the utility model one embodiment proposes;

[0015] Figure 4 It is the structural schematic diagram of sample detection method that the utility model one embodiment proposes;

[0016] Figure 5 It is the structural schematic diagram of sample detection method that the utility model another embodiment proposes;

[0017] Figure 6 It is the structural schematic diagram of sample detection method that the utility model another embodiment proposes;

[0018] Figure 7 It is the horizontal distribution schematic diagram in the one layer space of sample analyzer that the utility model embodiment one provides;

[0019] Figure 8 It is the distribution schematic diagram of first consumable supply device, second consumable supply device and reagent storage device that the utility model embodiment one provides;

[0020] Figure 9 It is the sectional view schematic diagram of extraction piece that the utility model one embodiment proposes;

[0021] Figure 10is Figure 9 a partial enlarged view at A in FIG.

[0022] Figure 11 is Figure 9 a partial enlarged view at B in FIG.

[0023] Figure 12 is Figure 9 a partial enlarged view at C in FIG.

[0024] Figure 13 is a partial sectional view of the extraction piece according to an embodiment of the present application;

[0025] Figure 14 is a perspective view of the extraction piece according to an embodiment of the present application;

[0026] Figure 15 is a top view of the extraction piece according to an embodiment of the present application;

[0027] Figure 16 is a view showing the cooperation of a plurality of extraction pieces according to an embodiment of the present application;

[0028] Figure 17 is a view showing the cooperation of a plurality of extraction pieces according to an embodiment of the present application;

[0029] Figure 18 is a view showing the cooperation of a plurality of extraction pieces according to an embodiment of the present application;

[0030] Figure 19 is a view showing the cooperation of a plurality of extraction pieces according to an embodiment of the present application. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, other embodiments obtained by those skilled in the art without creative work fall within the scope of the present application.

[0032] As Figures 1 to 4As shown, the embodiment of the utility model discloses a sample detection device 1000, the sample detection device 1000 including reagent storage assembly 201, reagent dispensing assembly 202, sample dispensing assembly 203, extraction assembly 204, amplification assembly 205, detection assembly 206, first liquid transfer assembly 207, scheduling assembly 208 and controller 209 are proposed, the sample detection device 1000 still forms reagent dispensing station, sample dispensing station, extraction station, liquid transfer station, amplification station and detection station, reagent storage assembly 201 is at least used to store first reagent container and second reagent container, first reagent container is used to store first reagent, and second reagent container is used to store second reagent, reagent dispensing assembly 202 is used to the extraction piece 100 in reagent dispensing station is dispensed with the first reagent container stored first reagent and the second reagent container stored second reagent, sample dispensing assembly 203 is used to the extraction piece 100 in sample dispensing station is dispensed with sample, extraction assembly 204 is used to the liquid in the extraction piece 100 in extraction station is extracted nucleic acid, first liquid transfer assembly 207 is used to the liquid in the extraction piece 100 in liquid transfer station is transferred to the amplification tube in liquid transfer station, amplification assembly 205 is used to the liquid in the amplification tube in amplification station is amplified processing, detection assembly 206 is used to the liquid in the amplification tube in detection station is detected, scheduling assembly 208 is used to the extraction piece 100 between reagent dispensing station, sample dispensing station, extraction station and liquid transfer station is scheduled, and scheduling assembly 208 is also used to the amplification tube between liquid transfer station, amplification station and detection station is scheduled, and controller 209 is configured as:

[0033] Step S11, control reagent dispensing assembly 202 to reagent storage assembly 201 in the first reagent container in the first reagent is dispensed to the extraction piece 100 in reagent dispensing station first cavity 101, and the second reagent in the second reagent container in reagent storage assembly 201 is dispensed to the second cavity 102 in the extraction piece 100 in reagent dispensing station.

[0034] In one embodiment, reagent storage assembly 201 includes reagent disc, and the reagent disc is provided with a plurality of positions for carrying reagent containers in a disc structure. Reagent storage assembly 201 can rotate and drive the reagent containers carried thereby to rotate to a specific position, for example, a position for being aspirated by reagent dispensing assembly 202. In another embodiment, reagent storage assembly 201 is not in a disc structure and is not rotatable, for example, is a fixed rectangular structure, and reagent storage assembly 201 is provided with a plurality of reagent container positions, and reagent containers are placed in the reagent container positions.

[0035] In an embodiment, the reagent dispensing assembly 202 can include a reagent needle, which is driven by a two-dimensional or three-dimensional driving mechanism to move in a two-dimensional or three-dimensional space, so that the reagent needle can move to suck the reagent in the reagent container carried by the reagent storage assembly 201.

[0036] In step S12, the control scheduling assembly 208 schedules the extraction piece 100 from the reagent dispensing station to the sample dispensing station, and controls the sample dispensing assembly 203 to dispense the sample into the first cavity 101.

[0037] In an embodiment, the sample dispensing assembly 203 can include a sample needle, which is driven by a two-dimensional or three-dimensional driving mechanism to move in a two-dimensional or three-dimensional space, so that the sample needle can move to suck the sample at the sample suction site, and move to the sample dispensing station to dispense the sucked sample into the first cavity 101.

[0038] In step S13, the control scheduling assembly 208 schedules the extraction piece 100 located at the sample dispensing station and having the sample dispensed to the extraction station, and controls the extraction assembly 204 to extract nucleic acid from the liquid in the first cavity 101.

[0039] In step S14, the control scheduling assembly 208 transfers the extraction piece 100 in which the nucleic acid extraction is completed at the extraction station to the liquid transfer station, controls the first liquid transfer assembly 207 to transfer at least part of the liquid in which the nucleic acid extraction is completed in the first cavity 101 to the amplification tube located at the liquid transfer station, and then controls the first liquid transfer assembly 207 to transfer the second reagent in the second cavity 102 to the amplification tube located at the liquid transfer station.

[0040] In an embodiment, the scheduling assembly 208 includes a first transport frame, the first transport frame is provided with an extraction piece site and an amplification tube site, the extraction piece site is used to place the extraction piece 100, a magnet is arranged at the extraction piece site, the magnet is used to perform a magnetic attraction operation on the extraction piece 100 located at the extraction piece site, so as to gather the magnetic beads in the extraction piece 100 to the bottom wall or the side wall of the first cavity 101 of the extraction piece 100, and the amplification tube site is used to place the amplification tube. After the nucleic acid extraction is completed at the extraction station, the scheduling assembly 208 transfers the extraction piece 100 to the extraction piece site on the first transport frame, then the first transport frame moves to the liquid transfer station, and then the first liquid transfer assembly 207 transfers at least part of the liquid in which the nucleic acid extraction is completed in the first cavity 101 to the amplification tube at the liquid transfer station, and then controls the first liquid transfer assembly 207 to transfer the second reagent in the second cavity 102 to the amplification tube at the liquid transfer station.

[0041] In step S15, the control scheduling component 208 schedules the amplification tube to the amplification station, controls the amplification component 205 to perform amplification processing on the liquid in the amplification tube, and controls the control scheduling component 208 to schedule the amplification tube after the amplification processing is completed in the amplification station to the detection station, and controls the detection component 206 to perform detection on the liquid in the amplification tube in the detection station.

[0042] The amplification processing can be performed by using a polymerase chain reaction (PCR) method, a loop mediated isothermal amplification (LAMP) method, an isothermal chimeric primer initiated amplification (ICAN) method, a nuclear acid sequence-based amplification (NASBA) method, a strand displacement amplification (SDA) method, a ligase chain reaction (LCR) method, or a rolling circle amplification (RCA) method.

[0043] In an embodiment, the detection component 206 includes a light measurement component for performing light measurement on the reaction liquid after the incubation is completed to obtain reaction data of the sample. For example, the light measurement component detects the luminescence intensity of the reaction liquid to be measured, and calculates the concentration of the component to be measured in the sample by using a calibration curve.

[0044] In the embodiment, the second reagent required in the process of transferring the liquid after the nucleic acid extraction in the first cavity 101 to the amplification tube is injected into the second cavity 102 of the extraction piece 100 in the reagent dispensing station, and no additional mechanism for injecting the second reagent into the amplification tube needs to be added in the liquid transfer station, which can simplify the structure of the device and reduce the size of the device. Moreover, the degree of contamination of the stations downstream of the sample dispensing station is relatively high, and in the embodiment, the reagent dispensing component 202 for dispensing the second reagent is arranged upstream of the sample dispensing station, which can effectively reduce the risk of contamination of the reagent dispensing component 202 and improve the accuracy of sample detection. In addition, compared with the first liquid transfer component 207 that sucks the second reagent from the additional mechanism for injecting the second reagent into the amplification tube and injects the sucked second reagent into the amplification tube, the first liquid transfer component 207 in the embodiment sucks the second reagent from the second cavity 102 of the extraction piece 100 and then transfers the second reagent to the amplification tube, and the path is shorter and the time is less, which can improve the analysis efficiency of the sample.

[0045] like Figure 1 and Figure 3 As shown, in one embodiment, the sample detection device 1000 further includes a first loading assembly 210, which is used to load the first pipette 21 in the third cavity 103 of the extractor 100 onto the first liquid transfer assembly 207, so that the first liquid transfer assembly 207 can aspirate liquid through the first pipette 21. In one embodiment, the first loading assembly 210 is used to drive the first liquid transfer assembly 207 to move in a vertical direction and at least one horizontal direction. The first loading assembly 210 first drives the first liquid transfer assembly 207 to move horizontally above the third cavity 103 of the extractor 100, and then drives the first liquid transfer assembly 207 to descend vertically, thus completing the loading of the first pipette 21 by inserting the end of the first liquid transfer assembly 207 into the opening end of the first pipette 21. In another embodiment, the first loading assembly 210 is used to drive the first liquid transfer assembly 207 to move vertically and in two mutually perpendicular horizontal directions. In this embodiment, by setting the pipette head used by the first liquid transfer component 207 to be directly loaded from the third cavity 103 of the extractor 100 during liquid transfer, the sample detection device 1000 does not need to add a pipette head loading mechanism and scheduling component 208, which can simplify the device structure and reduce the device size.

[0046] In one embodiment, the process of controlling the first liquid transfer assembly 207 to transfer at least a portion of the liquid after nucleic acid extraction in the first cavity 101 to the amplification tube of the liquid transfer station is as follows: the first loading assembly 210 is controlled to load the first pipette head 21 in the third cavity 103 of the extractor 100 to the end of the first liquid transfer assembly 207, and then the first liquid transfer assembly 207 is controlled to aspirate at least a portion of the liquid after nucleic acid extraction in the first cavity 101 and transfer the aspirated liquid to the amplification tube of the liquid transfer station.

[0047] like Figures 1 to 3As shown, in one embodiment, the reagent storage assembly 201 is also used to store a third reagent container, the third reagent container is used to store an elution reagent, before the extraction piece 100 is dispatched to the sample dispensing station, the controller 209 also controls the reagent dispensing assembly 202 to dispense the elution reagent in the third reagent container in the reagent storage assembly 201 to the fourth cavity 104 of the extraction piece 100, the first reagent includes a magnetic bead reagent; the extraction assembly 204 includes a carrier 2041, an incubation piece 2042, a first magnetic attraction piece 2043, a first pipetting piece 2044, and a second pipetting piece 2045, the carrier 2041 is provided with a liquid suction station and an elution station, the carrier 2041 is used to carry the extraction piece 100, the first magnetic attraction piece 2043 is used to perform a magnetic attraction operation on the extraction piece 100 located at the liquid suction station, so as to gather the magnetic beads in the extraction piece 100 to the bottom wall or the side wall of the first cavity 101 of the extraction piece 100, the first pipetting piece 2044 is used to perform a liquid suction operation on the liquid in the first cavity 101 of the extraction piece 100 located at the liquid suction station, and the second pipetting piece 2045 is used to inject the elution reagent in the fourth cavity 104 of the extraction piece 100 into the first cavity 101 of the extraction piece 100 located at the elution station, and the control extraction assembly 204 performs nucleic acid extraction on the liquid in the first cavity 101, including: controlling the dispatching assembly 208 to dispatch the extraction piece 100 located at the sample dispensing station to the incubation piece 2042 for incubation; controlling the dispatching assembly 208 to dispatch the extraction piece 100 after incubation to the liquid suction station, and controlling the first pipetting piece 2044 to perform a liquid suction operation on the liquid in the first cavity 101 of the extraction piece 100; controlling the dispatching assembly 208 to dispatch the extraction piece after completing the liquid suction operation to the elution station, and controlling the second pipetting piece 2045 to inject the elution reagent in the fourth cavity 104 of the extraction piece 100 into the first cavity 101 of the extraction piece 100.

[0048] That is, in the present embodiment, after the sample and the first reagent are incubated, the first pipetting piece 2044 sucks out the waste liquid in the first cavity 101, and then the second pipetting piece 2045 sucks the elution reagent and injects the sucked elution reagent into the first cavity 101.

[0049] As Figures 1 to 3As shown, in one embodiment, the sample detection device 1000 further includes a second loading assembly 211, which is used to load the first pipette tip 21 in the third cavity 103 of the extractor 100 onto the second pipette tip 2045, so that the second pipette tip 2045 can aspirate liquid through the first pipette tip 21. In one embodiment, the second loading assembly 211 is used to drive the second pipette tip 2045 to move in a vertical direction and at least one horizontal direction. The second loading assembly 211 first drives the second pipette tip 2045 to move horizontally above the third cavity 103 of the extractor 100, and then drives the second pipette tip 2045 to descend vertically, so that the end of the second pipette tip 2045 is inserted into the opening end of the first pipette tip 21, thus completing the loading of the first pipette tip 21. In another embodiment, the second loading assembly 211 is used to drive the second pipette tip 2045 to move in a vertical direction and two mutually perpendicular horizontal directions. In this embodiment, by setting the second pipette 2045 to be loaded directly from the third cavity 103 of the extractor 100 when transferring liquid, the sample detection device 1000 does not need to add a pipette loading mechanism and scheduling component 208, which can simplify the device structure and reduce the device size.

[0050] In one embodiment, the process of controlling the second pipette 2045 to inject the elution reagent in the fourth chamber 104 of the extractor 100 into the first chamber 101 of the extractor 100 located at the elution station is as follows: the second loading assembly 211 is controlled to load the first pipette head 21 in the third chamber 103 of the extractor 100 onto the end of the second pipette 2045, and then the second pipette 2045 is controlled to extract the elution reagent from the fourth chamber 104 of the extractor 100 and inject the aspirated elution reagent into the first chamber 101 of the extractor 100 located at the elution station.

[0051] like Figure 1 and Figure 3As shown, in one embodiment, the sample detection device 1000 further includes a cleaning fluid supply component 212, the extraction component 204 further includes a second magnetic suction member 2046 and a third pipetting member 2047, and the carrier member 2041 is further provided with a cleaning station. The cleaning fluid supply component 212 is used to inject cleaning fluid into the first cavity 101 of the extraction component located at the cleaning station. The second magnetic suction member 2046 is used to perform a magnetic suction operation on the extraction component 100 located at the cleaning station to gather the magnetic beads in the extraction component 100 onto the bottom wall or side wall of the first cavity 101 of the extraction component 100. The third pipetting member 2047... The controller 209 is configured to: control the scheduling component 208 to schedule the extractor 100, which has completed the liquid suction operation, to the cleaning station; control the cleaning fluid supply component 212 to inject cleaning fluid into the extractor located at the cleaning station; and control the third pipetting component 2047 to perform a liquid suction operation on the liquid in the first cavity 101 of the extractor 100 after the magnetic suction operation is completed to absorb the cleaning fluid; and control the scheduling component 208 to schedule the extractor 100, which has completed the liquid suction operation, to the elution station.

[0052] That is, in this embodiment, after the first pipette 2044 aspirates the waste liquid in the first cavity 101 and before the second pipette 2045 aspirates the elution reagent and injects the aspirated elution reagent into the first cavity 101, the cleaning liquid is injected and aspirated into the first cavity 101.

[0053] like Figure 1 and Figure 3 As shown, in one embodiment, the sample detection device 1000 further includes a second loading assembly 211, which is used to load the second pipette head 22 in the fifth cavity 105 of the extractor 100 onto the first pipette head 2044 or the third pipette head 2047, so that the first pipette head 2044 or the third pipette head 2047 can draw liquid through the second pipette head 22.

[0054] In one embodiment, the process of the first pipette 2044 performing aspiration operation on the first cavity 101 of the extractor 100 at the aspiration station is as follows: the second loading assembly 211 is controlled to load the second pipette head 22 in the fifth cavity 105 of the extractor 100 to the end of the first pipette 2044, and then the first pipette 2044 is controlled to aspirate the liquid in the first cavity 101 of the extractor 100 at the aspiration station.

[0055] In one embodiment, the process of controlling the third pipette 2047 to draw the cleaning fluid is as follows: the second loading assembly 211 is controlled to load the second pipette head 22 in the fifth cavity 105 of the extractor 100 to the end of the third pipette 2047, and then the third pipette 2047 is controlled to draw the cleaning fluid in the first cavity 101 of the extractor at the cleaning station.

[0056] In one embodiment, the first pipette 2044 and the third pipette 2047 are the same liquid transfer assembly.

[0057] like Figures 1 to 3 As shown, in one embodiment, the controller 209 is further configured to control the first pipette 2044 and the third pipette 2047 to transfer the aspirated liquid to the sixth chamber 106 of the extraction member 100. In this embodiment, the first pipette 2044 and the third pipette 2047 travel a shorter path during waste liquid discharge, thereby shortening the waste liquid discharge time and improving sample analysis efficiency. Of course, this is not limited to the above embodiment. For example, in another embodiment, the sample detection device 1000 also includes a waste liquid collection device, and the first pipette 2044 and the third pipette 2047 can also transfer the aspirated liquid to the waste liquid collection device.

[0058] In one embodiment, the sample detection device 1000 further includes a capping assembly for gripping the cap of the amplification tube and closing the cap onto the open end of the amplification tube. After the first liquid transfer assembly 207 transfers the second reagent from the second chamber 102 into the amplification tube, the controller 209 is further configured to control the capping assembly to close the cap onto the open end of the amplification tube located at the liquid transfer station. In this embodiment, by using a cap to close the open end of the amplification tube, the large-scale evaporation of nucleic acid-containing liquid within the amplification tube during amplification can be effectively reduced, thereby reducing cross-contamination.

[0059] In one embodiment, the second reagent is used to seal the nucleic acid-containing liquid within the amplification tube. In this embodiment, the second reagent can reduce the excessive evaporation of the nucleic acid-containing liquid within the amplification tube during the amplification process, thereby reducing cross-contamination. The second reagent can be, but is not limited to, silicone oil.

[0060] In one embodiment, the first reagent includes at least two, and the control reagent dispensing assembly 202 dispenses the first reagent in the first reagent container in the reagent storage assembly 201 into the first cavity 101 in the extraction piece 100 by: the control reagent dispensing assembly 202 sequentially aspirating the at least two first reagents from the first reagent container, and then the control reagent dispensing assembly 202 injecting the aspirated at least two first reagents into the first cavity 101 at one time. Here, the "sequential aspiration" refers to aspirating one of the at least two first reagents and then aspirating another of the at least two first reagents, and does not limit the order of aspiration of the first reagents. With this embodiment, the at least two first reagents are injected into the first cavity 101 in the extraction piece 100 by multi-aspiration and one injection, which can effectively shorten the dispensing time of the first reagent, thereby shortening the analysis time of the sample and improving the analysis efficiency. Of course, in other embodiments, the reagent dispensing assembly 202 can also inject the at least two first reagents into the first cavity 101 in the extraction piece 100 by one aspiration and one injection, which can be determined according to actual design needs.

[0061] In one embodiment, the first reagent includes a lysis solution, an IC (inner control) reagent, a magnetic bead reagent, and a proteinase k, and the control reagent dispensing assembly 202 dispenses the first reagent in the first reagent container in the reagent storage assembly 201 into the first cavity 101 in the extraction piece 100 by: the control reagent dispensing assembly 202 sequentially aspirating the lysis solution, the IC reagent, the magnetic bead reagent, and the proteinase k from the first reagent container, and then the control reagent dispensing assembly 202 injecting the aspirated lysis solution, IC reagent, magnetic bead reagent, and proteinase k into the first cavity 101 at one time.

[0062] In one embodiment, the sample detection device 1000 further includes a housing and a partition plate, the housing has a receiving cavity, and the partition plate is arranged in the receiving cavity to divide the receiving cavity into a reagent cavity and a sample cavity, the reagent dispensing assembly 202 is located in the reagent cavity, the sample dispensing assembly 203 is located in the sample cavity, and the partition plate is provided with an opening, and the opening is used to dispatch the extraction piece 100 from the reagent dispensing station to the sample dispensing station by the dispatching assembly 208. With this embodiment, the reagent area and the sample area can be separated to avoid contamination of the sample area to the reagent area.

[0063] As Figure 1 , Figure 5 and Figure 6As shown in FIG. 1, in one embodiment, the sample detection device 1000 further comprises a first consumable supply device 213 and a second consumable supply device 214. The first consumable supply device 213 is at least used to provide the extraction piece 100. The second consumable supply device 214 is at least used to provide the amplification tube. The sample dispensing assembly 203 is used to aspirate at least part of the sample from the sample container and dispense into the extraction piece 100 provided by the first consumable supply device 213. The extraction assembly 204 is used to perform nucleic acid extraction on the liquid containing at least the sample in the extraction piece 100 to obtain the nucleic acid extraction liquid. In this embodiment, the extraction piece 100 is provided by the first consumable supply device 213 and the amplification tube is provided by the second consumable supply device 214, so that the continuous detection requirement of batch samples can be met, and the situation that the sample detection efficiency is affected due to the lack of amplification extraction piece 100 and reaction container can be avoided.

[0064] As shown in FIG. 1, in one embodiment, the sample detection device 1000 further comprises a sample storage assembly 215, which is at least used to store the sample container loaded with the sample. The sample container can be manually loaded into the sample storage device by the user, or the sample can be automatically loaded by the automatic sample loading device. The sample dispensing assembly 203 is used to aspirate at least part of the sample from the sample container provided by the sample storage assembly 215 and dispense into the extraction piece 100 provided by the first consumable supply device 213. Figure 1 Figure 5 As shown in FIG. 1, in one embodiment, the sample detection device 1000 further comprises a sample storage assembly 215, which is at least used to store the sample container loaded with the sample. The sample container can be manually loaded into the sample storage device by the user, or the sample can be automatically loaded by the automatic sample loading device. The sample dispensing assembly 203 is used to aspirate at least part of the sample from the sample container provided by the sample storage assembly 215 and dispense into the extraction piece 100 provided by the first consumable supply device 213.

[0065] As shown in FIG. 1, in one embodiment, the sample detection device 1000 further comprises a sample storage assembly 215, which is at least used to store the sample container loaded with the sample. The sample container can be manually loaded into the sample storage device by the user, or the sample can be automatically loaded by the automatic sample loading device. The sample dispensing assembly 203 is used to aspirate at least part of the sample from the sample container provided by the sample storage assembly 215 and dispense into the extraction piece 100 provided by the first consumable supply device 213. Figure 5 ​As shown, in one embodiment, the scheduling assembly 208 comprises a first consumable conveying assembly 2081 and a second consumable conveying assembly 2082, the first consumable conveying assembly 2081 is configured to carry the extraction piece 100 to move linearly along the first horizontal direction P1, and the second consumable conveying assembly 2082 is configured to carry the amplification tube to move linearly along the first horizontal direction P1, the first consumable conveying assembly 2081 and the second consumable conveying assembly 2082 are arranged side by side along the second horizontal direction P2, and the second horizontal direction P2 is substantially perpendicular to the first horizontal direction P1. The first consumable conveying assembly 2081 and the second consumable conveying assembly 2082 are two consumable conveying assemblies that can independently convey consumables, that is, the first consumable conveying assembly 2081 and the second consumable conveying assembly 2082 can work in parallel. The first consumable conveying assembly 2081 and the second consumable conveying assembly 2082 are arranged side by side along the second horizontal direction P2, specifically, the first consumable conveying assembly 2081 and the second consumable conveying assembly 2082 are distributed along the second horizontal direction P2, and the first consumable conveying assembly 2081 and the second consumable conveying assembly 2082 are substantially parallel. The first consumable conveying assembly 2081 and the second consumable conveying assembly 2082 can be in contact with each other or have a spacing, as long as it does not affect the conveying of the consumables carried by the two. The second horizontal direction P2 is substantially perpendicular to the first horizontal direction P1, including: the angle formed by the intersection of the second horizontal direction P2 and the first horizontal direction P1 is 90°, and the angle formed by the intersection of the second horizontal direction P2 and the first horizontal direction P1 is slightly larger or slightly smaller than 90° within a certain range. The first consumable conveying assembly 2081 and the second consumable conveying assembly 2082 are substantially parallel, including: the first consumable conveying assembly 2081 and the second consumable conveying assembly 2082 are parallel to each other, and the first consumable conveying assembly 2081 and the second consumable conveying assembly 2082 have a certain small range of inclination angle. The present embodiment sets the scheduling assembly 208 to comprise the first consumable conveying assembly 2081 and the second consumable conveying assembly 2082, so that at least part of the extraction piece 100 and the amplification tube can be scheduled in parallel, which is beneficial to improve the scheduling efficiency.

[0066] As Figure 5As shown, in one embodiment, the first consumable conveying assembly 2081 has a first side and a second side oppositely arranged along the second horizontal direction P2, the second consumable conveying assembly 2082 has a first side and a second side oppositely arranged along the second horizontal direction P2, the second side of the first consumable conveying assembly 2081 is adjacent to the first side of the second consumable conveying assembly 2082, at least part of at least one of the sample storage assembly 215, the extraction assembly 204 and the amplification assembly 205 is located on the first side of the first consumable conveying assembly 2081, and at least part of at least another one of the sample storage assembly 215, the extraction assembly 204 and the amplification assembly 205 is located on the second side of the second consumable conveying assembly 2082. In this embodiment, the consumable conveying assemblies of the extraction part 100 and the amplification tube are arranged in the middle region, and at least part of at least two of the sample storage assembly 215, the extraction assembly 204 and the amplification assembly 205 is arranged on the opposite side of the middle region, that is, two consumable conveying lines are arranged in the middle, and the functional modules are distributed on the opposite sides of the two consumable conveying lines, so that the two consumable conveying lines can simultaneously meet the consumable scheduling requirements of the functional modules on both sides, without the need to arrange a consumable conveying assembly for each functional module, which is beneficial to simplify the consumable scheduling network of the sample detection device 1000, and further beneficial to improve the scheduling efficiency of the nucleic acid extraction container and the amplification tube between the functional modules, and beneficial to reduce the volume and cost of the sample detection device 1000.

[0067] As Figure 5As shown, in one embodiment, the sample storage component 215 is located on the first side of the first consumable conveying component 2081, and at least part of at least one of the extraction component 204 and the amplification component 205 is located on the second side of the second consumable conveying component 2082, i.e., the first consumable conveying component 2081 is located between the sample storage component 215 and the second consumable conveying component 2082 along the second horizontal direction P2. In this embodiment, the first consumable conveying component 2081 is arranged closer to the sample storage component 215 than the second consumable conveying component 2082, and the second consumable conveying component 2082 is arranged closer to at least one of the extraction component 204 and the amplification component 205 than the first consumable conveying component 2081. Since the samples stored in the sample storage component 215 are mainly used for dispensing in the extraction component 100, arranging the first consumable conveying component 2081 used for scheduling the extraction component 100 closer to the sample storage component 215 can help to reduce the movement stroke of the sample dispensing component 203 when dispensing samples, thereby helping to improve the detection efficiency of the sample detection device 1000 and reduce the risk of sample contamination. Of course, in specific applications, the relative positional relationship of the sample storage component 215, the extraction component 204, the amplification component 205, the first consumable conveying component 2081 and the second consumable conveying component 2082 is not limited to this, for example, as an alternative embodiment, the relative positional relationship of the sample storage component 215, the extraction component 204, the amplification component 205, the first consumable conveying component 2081 and the second consumable conveying component 2082 can also be that the sample storage component 215 is located on the second side of the second consumable conveying component 2082, and at least part of at least one of the extraction component 204 and the amplification component 205 is located on the first side of the first consumable conveying component 2081.

[0068] As Figure 5As shown, in one embodiment, at least part of two of the sample storage component 215, the extraction component 204 and the amplification component 205 are distributed along the same side of the scheduling component 208, i.e. at least part of two of the sample storage component 215, the extraction component 204 and the amplification component 205 are distributed side by side along the first horizontal direction P1 and are distributed along the same side of the scheduling component 208. In this embodiment, the sample storage component 215, the extraction component 204 and the amplification component 205 are all distributed around the scheduling component 208. Since two of the sample storage component 215, the extraction component 204 and the amplification component 205 are distributed along the first horizontal direction P1, at least part of two of them are distributed along the second horizontal direction P2 on the opposite side of the scheduling component 208, i.e. two of the sample storage component 215, the extraction component 204 and the amplification component 205 are distributed along the first horizontal direction P1 and two of them are distributed along the second horizontal direction P2, instead of being arranged in a straight line along one horizontal direction, so that the size of the sample detection device 1000 in a single horizontal direction can be effectively reduced, thereby facilitating the space utilization of the molecular laboratory.

[0069] As shown in FIG. 1, the sample detection device 1000 comprises a scheduling component 208, a sample storage component 215, an extraction component 204 and an amplification component 205. Figure 5As shown, in one embodiment, the sample storage component 215 is located on the first side of the first consumable delivery component 2081, and the extraction component 204 and the amplification component 205 are both at least partially located on the second side of the second consumable delivery component 2082, with the extraction component 204 and the amplification component 205 arranged side by side along the first horizontal direction P1. In this embodiment, the scheduling component 208 has the sample storage component 215 on one side of its opposite sides, and at least a portion of the extraction component 204 and at least a portion of the amplification component 205 on the other side. This achieves the goal of reducing the size of the sample detection device 1000 in a single horizontal direction, and also shortens the journey of the nucleic acid extract obtained by the extraction component 204 to the amplification component 205, thereby improving the detection efficiency of the sample detection device 1000, and also reducing sample contamination of the nucleic acid extraction system and the amplification reaction system in the sample area. Of course, in specific applications, the distribution of the sample storage component 215, extraction component 204, amplification component 205, first consumable delivery component 2081, and second consumable delivery component 2082 is not limited to this. For example, as an alternative implementation, the sample storage component 215 and extraction component 204 are at least partially located on the first side of the first consumable delivery component 2081, while the amplification component 205 is at least partially located on the second side of the second consumable delivery component 2082, and the sample storage component 215 and extraction component 204 are arranged side by side along the first horizontal direction P1; or, as another alternative implementation, the sample storage component 215 and amplification component 205 are at least partially located on the first side of the first consumable delivery component 2081, while the extraction component 204 is at least partially located on the second side of the second consumable delivery component 2082, and the sample storage component 215 and amplification component 205 are arranged side by side along the first horizontal direction P1.

[0070] like Figure 5 As shown, in one embodiment, the reagent storage component 201 and the sample storage component 215 are arranged side by side along a first horizontal direction P1, and are at least partially distributed on the same side of the scheduling component 208. In this embodiment, placing the reagent storage component 201 and the sample storage component 215 on the same side of the scheduling component 208 facilitates the loading of reagents and samples by operators or robots on the same side of the sample detection device 1000.

[0071] like Figure 5As shown, in one embodiment, the reagent storage component 201 and the sample storage component 215 are arranged side by side along a first horizontal direction P1 on the first side of the first consumable delivery component 2081. In this embodiment, the first consumable delivery component 2081 for scheduling the extraction unit 100 is arranged adjacent to the reagent storage component 201 and the sample storage component 215. The distance from the reagent storage component 201 and the sample storage component 215 to the first consumable delivery component 2081 is less than the distance to the second consumable delivery component 2082. This helps to reduce the travel distance of the sample dispensing component 203 from the sample storage component 215 to the extraction unit 100, and also helps to reduce the travel distance of the reagent dispensing device 102 from the reagent storage component 201 to the extraction unit 100, thereby improving the efficiency of sample and reagent dispensing.

[0072] like Figure 5 As shown, in one embodiment, the reagent storage component 201 includes a first reagent storage component 2011 and a second reagent storage component 2012, and the reagent dispensing component 202 includes a first reagent dispensing component and a second reagent dispensing component. The first reagent storage component 2011 stores first-class reagents, and the first reagent dispensing component draws first-class reagents from the first reagent storage component 2011 and dispenses them into the extraction unit 100. The extraction component 204 performs nucleic acid extraction on the liquid in the extraction unit 100, which contains at least the sample and the first-class reagents, to obtain a nucleic acid extract. The second reagent storage component 2012 stores second-class reagents, and the second reagent dispensing component draws second-class reagents from the second reagent storage component 2012 and dispenses them into an amplification tube. The amplification component 205 amplifies the liquid in the amplification tube, which contains at least the nucleic acid extract and the second-class reagents, to obtain a test solution. The first-class reagents are mainly used for nucleic acid extraction and are also called extraction reagents. The second-class reagents are mainly used for amplification reactions and are also called amplification reagents. In this embodiment, the extraction reagent is dispensed into the extraction unit 100 by aspiration and dispensing, and the amplification reagent is dispensed into the amplification tube by aspiration and dispensing. Of course, in specific applications, as an alternative embodiment, one of the extraction reagent and the amplification reagent can be pre-stored in a container, for example, the extraction reagent is pre-stored in the extraction unit 100, or the amplification reagent is pre-stored in the amplification tube.

[0073] like Figure 5As shown, in one embodiment, at least a portion of the second consumable supply device 214, the first reagent storage component 2011, the second reagent storage component 2012, and the sample storage component 215 are arranged side-by-side along a first horizontal direction P1. The first consumable delivery component 2081 extends past the sides of the second consumable supply device 214, the first reagent storage component 2011, the second reagent storage component 2012, and the sample storage component 215. In this embodiment, the consumable area, reagent area, and sample area are located on the same side of the sample detection device 1000. This facilitates reducing the dispensing distance for reagents and samples, and allows operators or robots to load amplification tubes, first-class reagents, second-class reagents, and samples onto the same side of the sample detection device 1000. Of course, in specific applications, the distribution of the second consumable supply device 214, the first reagent storage component 2011, the second reagent storage component 2012, and the sample storage component 215 is not limited to this. As an alternative implementation, at least one of the second consumable supply device 214, the first reagent storage component 2011, and the second reagent storage component 2012 may be located on the second side of the second consumable delivery component 2082. For example, the second consumable supply device 214 and / or the second reagent storage component 2012 may be located on the second side of the second consumable delivery component 2082.

[0074] like Figure 5 As shown, in one embodiment, at least a portion of the second consumable supply device 214, the first reagent storage component 2011, the second reagent storage component 2012, and the sample storage component 215 are arranged side-by-side along a first horizontal direction P1. Specifically, the first consumable delivery component 2081 extends sequentially from the side of the second consumable supply device 214, passing beside the first reagent storage component 2011, the second reagent storage component 2012, and the sample storage component 215. In this embodiment, the reagent area is located between the consumable area and the sample area. This facilitates reagent dispensing followed by sample dispensing, allowing consumables to be delivered to the reagent area for dispensing without passing through the sample area. After sample dispensing, the consumables no longer pass through the reagent area, preventing sample contamination of the reagent area.

[0075] Of course, in specific applications, the side-by-side arrangement of the second consumable supply device 214, the first reagent storage assembly 2011, the second reagent storage assembly 2012, and the sample storage assembly 215 is not limited to the above-mentioned scheme, for example, the following alternative embodiments can also be adopted: at least part of the second consumable supply device 214, the second reagent storage assembly 2012, the first reagent storage assembly 2011, and the sample storage assembly 215 are arranged side by side in the first horizontal direction PI in sequence; or, at least part of the second consumable supply device 214, the first reagent storage assembly 2011, the sample storage assembly 215, and the second reagent storage assembly 2012 are arranged side by side in the first horizontal direction PI in sequence; or, at least part of the second consumable supply device 214, the second reagent storage assembly 2012, the sample storage assembly 215, and the first reagent storage assembly 2011 are arranged side by side in the first horizontal direction PI in sequence; or, at least part of the second consumable supply device 214, the sample storage assembly 215, the first reagent storage assembly 2011, and the second reagent storage assembly 2012 are arranged side by side in the first horizontal direction PI in sequence; or, at least part of the second consumable supply device 214, the sample storage assembly 215, the second reagent storage assembly 2012, and the first reagent storage assembly 2011 are arranged side by side in the first horizontal direction PI in sequence; or, the first reagent storage assembly 2011, the second reagent storage assembly 2012, the second consumable supply device 214, and the sample storage assembly 215 are arranged side by side in the first horizontal direction PI in sequence; or, the second reagent storage assembly 2012, the first reagent storage assembly 2011, the second consumable supply device 214, and the sample storage assembly 215 are arranged side by side in the first horizontal direction PI in sequence; or, the first reagent storage assembly 2011, the second consumable supply device 214, the second reagent storage assembly 2012, and the sample storage assembly 215 are arranged side by side in the first horizontal direction PI in sequence; or, the second reagent storage assembly 2012, the second consumable supply device 214, the first reagent storage assembly 2011, and the sample storage assembly 215 are arranged side by side in the second horizontal direction P2 along the first horizontal direction PI in sequence.

[0076] As shown in FIG. 2A, in one embodiment, the sample storage assembly 215, the second reagent storage assembly 2012, the first reagent storage assembly 2011, the second consumable supply device 214, the amplification assembly 205, and the extraction assembly 204 are distributed around three sides of the scheduling assembly 208. Figure 5

[0077] Figure 6 ​​As shown, in one embodiment, the sample storage component 215, the second reagent storage component 2012, the first reagent storage component 2011, the second consumable supply device 214, the amplification component 205, and the extraction component 204 are arranged in a U-shape around three sides of the scheduling component 208. Specifically, the sample storage component 215, the second reagent storage component 2012, the first reagent storage component 2011, and the second consumable supply device 214 are arranged side-by-side along a first horizontal direction P1; the amplification component 205 and the extraction component 204 are also arranged along the first horizontal direction P1; the sample storage component 215, the second reagent storage component 2012, and the first reagent storage component 2011 are located on the first side of the first consumable delivery component 2081; the extraction component 204 and part of the amplification component 205 are located on the second side of the second consumable delivery component 2082; the second consumable supply device 214 extends from one side of the first reagent storage component 2011 toward the amplification component 205; and the part of the amplification component 205 extends toward the second consumable supply device 214.

[0078] like Figure 6 As shown, in one embodiment, the first consumable supply device 213 is located below the second consumable supply device 214, and extends from below the second consumable supply device 214 to below the reagent storage component 201. In this embodiment, the vertical space of the sample detection device 1000 is fully utilized, and the extraction element 100 and other space-consuming modules such as amplification tubes and reagents are designed vertically, thereby further reducing the horizontal space occupied by the sample detection device 1000. In addition, since the first consumable supply device 213, the second consumable supply device 214, the reagent storage component 201, and the sample storage component 215 are located on the same side of the sample detection device 1000, it is convenient for operators or robots to perform loading operations of the extraction element 100, amplification tubes, first-class reagents, second-class reagents, and samples on the same side of the sample detection device 1000.

[0079] like Figures 1 to 3 As shown, in one embodiment, the first consumable supply device 213 extends from directly below the second consumable supply device 214 to directly below the first reagent storage assembly 2011 and the second reagent storage assembly 2012.

[0080] In one embodiment, the sample detection device 1000 has an upper space and a lower space, the second consumable supply device 214, the first reagent storage assembly 2011, the second reagent storage assembly 2012, the first consumable conveying assembly 2081, the second consumable conveying assembly 2082, the extraction assembly 204, and the amplification assembly 205 are located in the upper space, and the first consumable supply device 213 is located in the lower space. In this embodiment, the storage of the extraction piece 100 is in the lower layer, and the storage of other consumables and the working position are in the upper layer, which can shorten the scheduling path of the consumables, simplify the structure of the mechanical hand, and thus reduce the horizontal occupation area of the entire sample detection device 1000, and increase the supply efficiency of the consumables.

[0081] As shown in Figure 7 、 Figures 1 to 3 The embodiment of the utility model discloses a sample detection device 1000, which comprises a reagent storage assembly 201, a reagent dispensing assembly 202, a sample dispensing assembly 203, an extraction assembly 204, an amplification assembly 205, a detection assembly 206, a first liquid transfer assembly 207, a scheduling assembly 208 and a controller 209. The sample detection device 1000 further comprises a reagent dispensing station, a sample dispensing station, an extraction station, a liquid transfer station, an amplification station and a detection station. The reagent storage assembly 201 is used for storing at least a first reagent container and a second reagent container. The first reagent container is used for storing a first reagent, and the second reagent container is used for storing a second reagent. The reagent dispensing assembly 202 is used for dispensing the first reagent stored in the first reagent container and the second reagent stored in the second reagent container to the extraction piece 100 located at the reagent dispensing station. The sample dispensing assembly 203 is used for dispensing a sample to the extraction piece 100 located at the sample dispensing station. The extraction assembly 204 is used for performing nucleic acid extraction on the liquid in the extraction piece 100 located at the extraction station. The first liquid transfer assembly 207 is used for transferring the liquid in the extraction piece 100 located at the liquid transfer station to an amplification tube located at the liquid transfer station. The amplification assembly 205 is used for performing amplification processing on the liquid in the amplification tube located at the amplification station. The detection assembly 206 is used for detecting the liquid in the amplification tube located at the detection station. The scheduling assembly 208 is used for scheduling the extraction piece 101 between the reagent dispensing station, the sample dispensing station, the extraction station and the liquid transfer station. The scheduling assembly 208 is also used for scheduling the amplification tube between the liquid transfer station, the amplification station and the detection station. The controller 209 is configured to:

[0082] In step S21, the reagent dispensing assembly 202 is controlled to dispense the first reagent in the first reagent container in the reagent storage assembly 201 to the first cavity 101 of the extraction piece 100 located at the reagent dispensing station, and to dispense the second reagent in the second reagent container in the reagent storage assembly 201 to the second cavity 102 of the extraction piece 100 located at the reagent dispensing station.

[0083] In step S22, the control scheduling component 208 schedules the extraction piece 100 from the reagent dispensing station to the sample dispensing station, and controls the sample dispensing component 203 to dispense the sample into the first cavity 101.

[0084] In step S23, the control scheduling component 208 schedules the extraction piece 100 located at the sample dispensing station and having the sample dispensed thereinto to the extraction station, and controls the extraction component 204 to perform nucleic acid extraction on the liquid in the first cavity 101, and the second reagent is transferred from the second cavity 102 to the first cavity 101 of the extraction piece during the nucleic acid extraction.

[0085] In step S24, the control scheduling component 208 transfers the extraction piece 100 having completed the nucleic acid extraction at the extraction station to the liquid transfer station, and controls the first liquid transfer component 207 to transfer at least part of the liquid in the first cavity 101 having completed the nucleic acid extraction to the amplification tube located at the liquid transfer station.

[0086] In step S25, the control scheduling component 208 schedules the amplification tube to the amplification station, controls the amplification component 205 to perform amplification processing on the liquid in the amplification tube, controls the control scheduling component 208 to schedule the amplification tube having completed the amplification processing at the amplification station to the detection station, and controls the detection component 206 to perform detection on the liquid in the amplification tube located at the detection station.

[0087] The sample detection device 1000 proposed in the embodiment can inject the second reagent required in the nucleic acid extraction process into the second cavity 102 of the extraction piece 100 at the reagent dispensing station, and does not need to additionally add a mechanism for injecting the second reagent into the extraction piece 100 at the extraction station, which can simplify the structure of the device and reduce the size of the device. Moreover, the downstream stations of the sample dispensing station are relatively highly contaminated, and in the embodiment, the reagent dispensing component 202 for dispensing the second reagent is arranged upstream of the sample dispensing station, which can effectively reduce the risk of contamination of the reagent dispensing component 202 and improve the accuracy of sample detection. In addition, compared with the first liquid transfer component 207 sucking the second reagent from the additionally added mechanism for injecting the second reagent into the extraction piece 100 and injecting the sucked second reagent into the first cavity 101 of the extraction piece 100, the second reagent is transferred from the second cavity 102 of the extraction piece 100 to the first cavity 101 of the extraction piece 100 in the embodiment, and the path is shorter and the time is less, so that the analysis efficiency of the sample can be improved.

[0088] In one embodiment, the sample detection device 1000 further comprises a first loading assembly 210, the first loading assembly 210 being configured to load the first pipette tip 21 in the third cavity 103 of the extraction piece 100 to the first liquid transfer assembly 207, so that the first liquid transfer assembly 207 sucks liquid through the first pipette tip 21.

[0089] In one embodiment, the second reagent is an elution reagent, and the first reagent comprises a magnetic bead reagent; the extraction assembly 204 further comprises a carrier 2041, an incubation piece 2042, a first magnetic attraction piece 2043, a first pipetting piece 2044, and a second pipetting piece 2045, the carrier 2041 is provided with a liquid suction station and an elution station, and the carrier 2041 is configured to carry the extraction piece 100, the first magnetic attraction piece 2043 is configured to perform a magnetic attraction operation on the extraction piece 100 located at the liquid suction station, so that the magnetic beads in the extraction piece 100 are gathered on the bottom wall or the side wall of the first cavity 101 of the extraction piece 100, the first pipetting piece 2044 is configured to perform a liquid suction operation on the liquid in the first cavity 101 of the extraction piece 100 located at the liquid suction station, and the second pipetting piece 2045 is configured to inject the elution reagent in the second cavity 102 of the extraction piece 100 into the first cavity 101 of the extraction piece 100 located at the elution station, and the control extraction assembly 204 performs nucleic acid extraction on the liquid in the first cavity 101, including: controlling the scheduling assembly 208 to schedule the extraction piece 100 located at the sample dispensing station to the incubation piece 2042 for incubation; controlling the scheduling assembly 208 to schedule the extraction piece 100 after incubation to the liquid suction station, and controlling the first pipetting piece 2044 to perform a liquid suction operation on the liquid in the first cavity 101 of the extraction piece 100; controlling the scheduling assembly 208 to schedule the extraction piece 100 after completing the liquid suction operation to the elution station, and controlling the second pipetting piece 2045 to inject the elution reagent in the second cavity 102 of the extraction piece 100 into the first cavity 101 of the extraction piece 100 located at the elution station.

[0090] In one embodiment, the sample detection device 1000 further comprises a second loading assembly 211, the second loading assembly 211 being configured to load the first pipette tip 21 in the third cavity 103 of the extraction piece 100 to the second pipetting piece 2045, so that the second pipetting piece 2045 sucks liquid through the first pipette tip 21.

[0091] In one embodiment, the sample detection apparatus 1000 further comprises a washing liquid providing assembly 212, the extraction assembly 204 further comprises a second magnetic attraction member 2046 and a third pipetting member 2047, the carrier 2041 is further provided with a washing station, the washing liquid providing assembly 212 is configured to inject washing liquid into the first cavity 101 of the extraction assembly 100 located at the washing station, the second magnetic attraction member 2046 is configured to perform magnetic attraction operation on the extraction assembly 100 located at the washing station, so as to gather the magnetic beads in the extraction assembly 100 to the bottom wall or the side wall of the first cavity 101 of the extraction assembly 100, the third pipetting member 2047 is configured to perform pipetting operation on the liquid in the first cavity 101 of the extraction assembly 100 located at the pipetting station, and the controller 209 is further configured to: control the scheduling assembly 208 to schedule the extraction assembly 100 located at the pipetting station and after the pipetting operation to the washing station; control the washing liquid providing assembly 212 to inject washing liquid into the extraction assembly 100 located at the washing station, and control the third pipetting member 2047 to perform pipetting operation on the liquid in the first cavity 101 of the extraction assembly 100 after the magnetic attraction operation; and control the scheduling assembly 208 to schedule the extraction assembly 100 after the pipetting operation to the elution station.

[0092] In one embodiment, the sample detection apparatus 1000 further comprises a second loading assembly 211, the second loading assembly 211 is configured to load the second pipetting head 22 in the fourth cavity 104 of the extraction assembly 100 to the first pipetting member 2044 or the third pipetting member 2047, so that the first pipetting member 2044 or the third pipetting member 2047 sucks liquid through the second pipetting head 22.

[0093] In one embodiment, the controller 209 is further configured to: control the first pipetting member 2044 and the third pipetting member 2047 to transfer the sucked liquid to the sixth cavity 106 of the extraction assembly 100.

[0094] In one embodiment, the first reagent comprises at least two, and the reagent dispensing assembly 202 comprises: controlling the reagent dispensing assembly 202 to sequentially suck the at least two first reagents from the first reagent container in the reagent storage assembly 201, and then controlling the reagent dispensing assembly 202 to inject the at least two first reagents into the first cavity 101 at one time.

[0095] In one embodiment, the sample detection apparatus 1000 further comprises a cap closing assembly configured to grab the cap of the amplification tube and close the grabbed cap to the open end of the amplification tube, and after the first liquid transferring assembly 207 transfers the second reagent in the second cavity 102 into the amplification tube, the controller 209 is further configured to: control the cap closing assembly to close the cap to the open end of the amplification tube located at the liquid transferring station.

[0096] The beneficial effects and other unrecorded embodiments of the various embodiments of the sample detection device 1000 proposed in the present embodiment can be referred to the above-mentioned embodiments, which will not be repeated here.

[0097] As shown in Figure 8 , Figure 3 The embodiments of the present application also propose a sample detection device 1000, which comprises a reagent storage assembly 201, a reagent dispensing assembly 202, a sample dispensing assembly 203, an extraction assembly 204, an amplification assembly 205, a detection assembly 206, a first liquid transfer assembly 207, a lid closing assembly, a scheduling assembly 208 and a controller 209. The sample detection device 1000 is also formed with a reagent dispensing station, a sample dispensing station, an extraction station, a liquid transfer station, an amplification station and a detection station. The reagent storage assembly 201 is used to store at least a first reagent container and a second reagent container. The first reagent container is used to store a first reagent, and the second reagent container is used to store a second reagent. The reagent dispensing assembly 202 is used to dispense the first reagent stored in the first reagent container and the second reagent stored in the second reagent container to the extraction piece 100 located at the reagent dispensing station. The sample dispensing assembly 203 is used to dispense a sample to the extraction piece 100 located at the sample dispensing station. The extraction assembly 204 is used to extract nucleic acid from the liquid in the extraction piece 100 located at the extraction station. The first liquid transfer assembly 207 is used to transfer the liquid in the extraction piece 100 located at the liquid transfer station to the amplification tube located at the liquid transfer station. The lid closing assembly is used to grab the tube cover of the amplification tube and close the grabbed tube cover to the open end of the amplification tube. The amplification assembly 205 is used to amplify the liquid in the amplification tube located at the amplification station. The detection assembly 206 is used to detect the liquid in the amplification tube located at the detection station. The scheduling assembly 208 is used to schedule the extraction piece 100 between the reagent dispensing station, the sample dispensing station, the extraction station and the liquid transfer station. The scheduling assembly 208 is also used to schedule the amplification tube between the liquid transfer station, the amplification station and the detection station. The controller 209 is configured to:

[0098] Step S31, control the reagent dispensing assembly 202 to dispense the first reagent in the first reagent container in the reagent storage assembly 201 to the first cavity 101 of the extraction piece 100 at the reagent dispensing station;

[0099] Step S32, control the scheduling assembly 208 to schedule the extraction piece 100 from the reagent dispensing station to the sample dispensing station, and control the sample dispensing assembly 203 to dispense the sample into the first cavity 101;

[0100] Step S33, the control scheduling component 208 schedules the extraction piece 100 located at the sample dispensing station and having the sample dispensed to the extraction station, and controls the extraction component 204 to perform nucleic acid extraction on the liquid in the first cavity 101.

[0101] Step S34, the control scheduling component 208 transfers the extraction piece 100 completing the nucleic acid extraction at the extraction station to the liquid transfer station, and controls the liquid transfer component to transfer at least part of the liquid in the first cavity 101 completing the nucleic acid extraction to the amplification tube located at the liquid transfer station.

[0102] Step S35, the control closing cap component closes the tube cap to the open end of the amplification tube located at the liquid transfer station.

[0103] Step S36, the control scheduling component 208 schedules the amplification tube to the amplification station, controls the amplification component 205 to perform amplification processing on the liquid in the amplification tube, controls the control scheduling component 208 to schedule the amplification tube completing the amplification processing at the amplification station to the detection station, and controls the detection component 206 to detect the liquid in the amplification tube located at the detection station.

[0104] The sample detection device 1000 provided in the embodiment of the utility model, after at least part of the liquid in the first cavity 101 completing nucleic acid extraction is transferred to the amplification tube at the liquid transfer station by the liquid transfer component, the tube cap is closed to the open end of the amplification tube at the liquid transfer station by the closing cap component, which can effectively reduce the large amount of volatilization of the liquid containing nucleic acid in the amplification tube during amplification, thereby reducing cross contamination.

[0105] In one embodiment, the sample detection device 1000 further comprises a first loading component 210, which is used to load the first pipette 21 in the third cavity 103 of the extraction piece 100 to the first liquid transfer component 207, so that the first liquid transfer component 207 sucks the liquid through the first pipette 21.

[0106] In one embodiment, the reagent storage assembly 201 is further configured to store a third reagent container for storing an elution reagent, and the controller 209 is further configured to control the reagent dispensing assembly 202 to dispense the elution reagent in the third reagent container in the reagent storage assembly 201 to the fourth cavity 104 of the extraction piece 100 before the extraction piece 100 is dispatched to the sample dispensing station, the first reagent includes magnetic bead reagent; the extraction assembly 204 includes a carrier 2041, an incubation piece 2042, a first magnetic attraction piece 2043, a first pipetting piece 2044, and a second pipetting piece 2045, the carrier 2041 is provided with a liquid suction station and an elution station, the carrier 2041 is configured to carry the extraction piece 100, the first magnetic attraction piece 2043 is configured to perform a magnetic attraction operation on the extraction piece 100 located at the liquid suction station, so as to gather the magnetic beads in the extraction piece 100 to the bottom wall or the side wall of the first cavity 101 of the extraction piece 100, the first pipetting piece 2044 is configured to perform a liquid suction operation on the liquid in the first cavity 101 of the extraction piece 100 located at the liquid suction station, and the second pipetting piece 2045 is configured to inject the elution reagent in the fourth cavity 104 of the extraction piece 100 into the first cavity 101 of the extraction piece 100 located at the elution station, and the control extraction assembly 204 performs nucleic acid extraction on the liquid in the first cavity 101, including: controlling the dispatching assembly 208 to dispatch the extraction piece 100 located at the sample dispensing station to the incubation piece 2042 for incubation; controlling the dispatching assembly 208 to dispatch the extraction piece 100 after incubation to the liquid suction station, and controlling the first pipetting piece 2044 to perform a liquid suction operation on the liquid in the first cavity 101 of the extraction piece 100; controlling the dispatching assembly 208 to dispatch the extraction piece after completing the liquid suction operation to the elution station, and controlling the second pipetting piece 2045 to inject the elution reagent in the fourth cavity 104 of the extraction piece 100 into the first cavity 101 of the extraction piece 100.

[0107] In one embodiment, the sample detection device 1000 further includes a second loading assembly 211 configured to load the first pipetting head 21 in the third cavity 103 of the extraction piece 100 to the second pipetting piece 2045, so that the second pipetting piece 2045 sucks liquid through the first pipetting head 21.

[0108] In one embodiment, the sample detection apparatus 1000 further comprises a washing liquid providing assembly 212, the extraction assembly 204 further comprises a second magnetic attraction member 2046 and a third pipetting member 2047, the carrier 2041 is further provided with a washing station, the washing liquid providing assembly 212 is configured to inject washing liquid into the first cavity 101 of the extraction assembly 100 located at the washing station, the second magnetic attraction member 2046 is configured to perform magnetic attraction operation on the extraction assembly 100 located at the washing station, so as to gather the magnetic beads in the extraction assembly 100 to the bottom wall or the side wall of the first cavity 101 of the extraction assembly 100, the third pipetting member 2047 is configured to perform pipetting operation on the liquid in the first cavity 101 of the extraction assembly 100 located at the washing station, and the controller 209 is further configured to: control the scheduling assembly 208 to schedule the extraction assembly after the pipetting operation to the washing station; control the washing liquid providing assembly 212 to inject washing liquid into the extraction assembly located at the washing station, and control the third pipetting member 2047 to perform pipetting operation on the liquid in the first cavity 101 of the extraction assembly 100 after the magnetic attraction operation; and control the scheduling assembly 208 to schedule the extraction assembly after the pipetting operation to the elution station.

[0109] In one embodiment, the sample detection apparatus further comprises a second loading assembly 211, the second loading assembly 211 is configured to load the second pipetting head 22 in the fifth cavity 105 of the extraction assembly 100 to the first pipetting member 2044 or the third pipetting member 2047, so that the first pipetting member 2044 or the third pipetting member 2047 sucks liquid through the second pipetting head 22.

[0110] In one embodiment, the controller 209 is further configured to: control the first pipetting member 2044 and the third pipetting member 2047 to transfer the sucked liquid to the sixth cavity 106 of the extraction assembly 100.

[0111] In one embodiment, the first reagent comprises at least two, and the reagent dispensing assembly 202 comprises: controlling the reagent dispensing assembly 202 to sequentially suck the at least two first reagents from the first reagent container, and then controlling the reagent dispensing assembly 202 to inject the at least two first reagents into the first cavity 101 at one time.

[0112] The beneficial effects of the various embodiments of the sample detection apparatus 1000 and other unrecorded embodiments proposed in the embodiments can be referred to the above-described embodiments, and will not be repeated here.

[0113] As Figure 3As shown, the embodiment of the utility model also proposes a kind of extracting piece 100, the extracting piece 100 is applied to molecular diagnostic device, and the shell of molecular diagnostic device is at least provided with reagent dispensing assembly and sample dispensing assembly, the extracting piece 100 includes open first cavity 101 and second cavity 102, first cavity 101 is used to accommodate the first reagent that reagent dispensing assembly injects and the sample that sample dispensing assembly injects, second cavity 102 is used to accommodate the second reagent that reagent dispensing assembly injects.

[0114] The extracting piece 100 proposed in the embodiment, by being provided with open first cavity 101 and second cavity 102, and first reagent and second reagent are subsequently injected into first cavity 101 and second cavity 102 in the process of molecular diagnosis by molecular diagnostic device, relative to the first reagent that the first cavity 101 of existing extracting piece 100 is pre-set and the second reagent that the second cavity 102 is pre-set, then the opening of first cavity 101 and the opening of second cavity 102 are sealed by the way of sealing film, the extracting piece 100 proposed in the embodiment does not need to consider the problem of sealing film puncture in the subsequent process of molecular diagnosis, and can play the role of simplifying process.

[0115] As shown in Figure 9 And Figure 3 In one embodiment, the extracting piece 100 also includes open third cavity 103 and fourth cavity 104, third cavity 103 is used to accommodate first pipette head 21, and fourth cavity 104 is used to accommodate second pipette head 22, and the volume of second pipette head 22 is greater than the volume of first pipette head 21.In one embodiment, the extracting piece 100 also includes open fifth cavity 105, and fifth cavity 105 is used to accommodate the third reagent injected by molecular diagnostic device.In one embodiment, the extracting piece 100 also includes open sixth cavity 106, and sixth cavity 106 is used to accommodate waste liquid.The extracting piece 100 proposed in the embodiment of the utility model, by integrating first cavity 101 to sixth cavity 106, all operations can be carried out on one extracting piece 100 in the extraction process of nucleic acid, to greatly reduce the number of consumables, production, packaging and transportation are relatively convenient.Moreover, all extraction operations are carried out on one extracting piece 100, which can simplify detection process and improve detection efficiency.

[0116] As shown in Figure 9 And Figure 10As shown, in one embodiment, the extraction member 100 further comprises a first pipette head 21 and a second pipette head 22, the first pipette head 21 is accommodated in the third cavity 103, and the second pipette head 22 is accommodated in the fourth cavity 104. In one embodiment, the volume of the first pipette head 21 is smaller than the volume of the second pipette head 22, for example, the volume of the first pipette head 21 is 300 μL, and the volume of the second pipette head 22 is 1000 μL. Of course, the volume of the first pipette head 21 and the volume of the second pipette head 22 can also be the same, which can be determined according to actual design needs.

[0117] As shown, Figure 10 In one embodiment, the side wall of the third cavity 103 near the opening of the third cavity 103 is provided with a first step 1031 or a first stop portion. The outer side wall of the first pipette head 21 has a first hanging portion 211, and when the first pipette head 21 is accommodated in the third cavity 103, the first step 1031 or the first stop portion abuts against the first hanging portion 211 of the first pipette head 21 to position the first pipette head 21. With this embodiment, through the positioning effect of the first step 1031 or the first stop portion and the first hanging portion 211, it can be avoided that the needle tip of the first pipette head 21 touches the bottom wall of the third cavity 103 when the first pipette head 21 is accommodated in the third cavity 103. Moreover, it is also beneficial for the liquid transfer assembly to take out the first pipette head 21 from the third cavity 103.

[0118] As shown, Figure 11 In one embodiment, the side wall of the fourth cavity 104 near the opening of the fourth cavity 104 is provided with a second step 1041 or a second stop portion. The outer side wall of the second pipette head 22 has a second hanging portion 221, and when the second pipette head 22 is accommodated in the fourth cavity 104, the second step 1041 or the second stop portion abuts against the second hanging portion 221 of the second pipette head 22 to position the second pipette head 22. With this embodiment, through the positioning effect of the second step 1041 or the second stop portion and the second hanging portion 221, it can be avoided that the needle tip of the second pipette head 22 touches the bottom wall of the fourth cavity 104 when the second pipette head 22 is accommodated in the fourth cavity 104. Moreover, it is also beneficial for the liquid transfer assembly to take out the second pipette head 22 from the fourth cavity 104.

[0119] As shown, Figure 12As shown, in one embodiment, the bottom of the third cavity 103 is provided with a first protrusion 1032. The first protrusion 1032 is used to contact the liquid hanging on the needle tip of the first pipette head 21 to reduce the amount of liquid hanging on the needle tip of the first pipette head 21. In one embodiment, when the first pipette head 21 is accommodated in the third cavity 103, the first protrusion 1032 is very close to the needle tip of the first pipette head 21, for example, 2-3 mm, and the liquid hanging on the needle tip of the first pipette head 21 will touch the protrusion and flow into the third cavity 103 under the guidance of the protrusion, so as to reduce the amount of liquid hanging on the needle tip of the first pipette head 21. With this embodiment, when the first pipette head 21 is used again subsequently, the liquid hanging on the first pipette head 21 can be prevented from falling on the extraction member 100 or the work station of the sample detection device 1000 to cause contamination of the first pipette head 21 or the sample detection device 1000. In one embodiment, the first protrusion 1032 is a conical structure, but is not limited to a conical structure, and can be other shapes, for example, a cross-shaped, semicircular or other irregular shape, which can be determined according to actual design needs, as long as the first protrusion 1032 can reduce the amount of liquid hanging on the needle tip of the first pipette head 21.

[0120] As shown, Figure 13 In one embodiment, the bottom of the fourth cavity 104 is provided with a second protrusion 1042. The second protrusion 1042 is used to contact the liquid hanging on the needle tip of the second pipette head 22 to reduce the amount of liquid hanging on the needle tip of the second pipette head 22. In one embodiment, when the second pipette head 22 is accommodated in the fourth cavity 104, the second protrusion 1042 is very close to the needle tip of the second pipette head 22, for example, 2-3 mm, and the liquid hanging on the needle tip of the second pipette head 22 will touch the protrusion and flow into the fourth cavity 104 under the guidance of the protrusion, so as to reduce the amount of liquid hanging on the needle tip of the second pipette head 22. With this embodiment, when the second pipette head 22 is used again subsequently, the liquid hanging on the second pipette head 22 can be prevented from falling on the extraction member 100 or the work station of the sample detection device 1000 to cause contamination of the second pipette head 22 or the sample detection device 1000. In one embodiment, the second protrusion 1042 is a conical structure, but is not limited to a conical structure, and can be other shapes, for example, a cross-shaped, semicircular or other irregular shape, which can be determined according to actual design needs, as long as the second protrusion 1042 can reduce the amount of liquid hanging on the needle tip of the first pipette head 21.

[0121] As shown, Figure 13As shown in the drawings, in one embodiment, the sidewall of the first cavity 101 is provided with a first flow guide structure 1011 extending from the opening of the first cavity 101 to the bottom of the first cavity 101. With this implementation, by providing the first flow guide structure 1011 on the sidewall of the first cavity 101 to guide the liquid injected into the first cavity 101, the formation of rolling of the liquid injected into the first cavity 101 can be avoided, and in turn the occurrence of aerosol can be avoided, reducing the risk of infection of the detection personnel.

[0122] As shown in the drawings, Figure 13 In some embodiments, the first cavity 101 has a first central axis L1, and the first flow guide structure 1011 includes a first inclined surface 1012 extending obliquely from the edge of the opening of the first cavity 101 towards the first central axis L1.

[0123] It should be noted that the first inclined surface 1012 is not limited to being provided at the opening edge of the first cavity 101, but can also be provided at the middle or bottom of the first cavity 101.

[0124] It should also be noted that the first flow guide structure 1011 is not limited to being provided in the form of the first inclined surface 1012. For example, in other embodiments, the first flow guide structure 1011 includes a second spiral surface extending spirally from the edge of the opening of the first cavity 101 around the first central axis L1 towards the bottom of the first cavity 101.

[0125] As shown in the drawings, Figure 13 In some embodiments, the inclination angle of the first inclined surface 1012 relative to the first central axis L1 is α1, and α1 = 15°-45°. For example, the inclination angle of the first inclined surface 1012 relative to the first central axis L1 is 15°, 20°, 25°, 30°, 35°, 40°, 45°, or any value between any two adjacent values of 15°, 20°, 25°, 30°, 35°, 40°, 45°. In this embodiment, the inclination angle of the first inclined surface 1012 relative to the first central axis L1 is 27.5°.

[0126] As shown in the drawings, Figure 13 In some embodiments, the wall surface of the first cavity 101 further includes a first wall surface 1013 and a second wall surface 1014, the first wall surface 1013 is connected between the first inclined surface 1012 and the second wall surface 1014, the second wall surface 1014 extends from the bottom end of the first wall surface 1013 to the bottom of the first cavity 101, the inclination angle of the first wall surface 1013 relative to the first central axis L1 is α2, and α2 = 0°-2°, the inclination angle of the second wall surface 1014 relative to the first central axis L1 is β3, and β3 = 5°-45°.

[0127] As shown in the drawings, Figure 13As shown, in some embodiments, the wall of the first cavity 101 further includes a first wall surface 1013, and the first flow guiding structure 1011 further includes an arc-shaped surface 1015. The arc-shaped surface 1015 connects the first inclined surface 1012 and the first wall surface 1013. The first wall surface 1013 extends from the arc-shaped surface 1015 toward the bottom of the first cavity 101, and the arc-shaped surface 1015 is used to achieve a smooth transition between the first inclined surface 1012 and the first wall surface 1013. In this embodiment, the liquid injected into the first cavity 101 can flow horizontally along the wall of the first cavity 101, avoiding liquid splashing on the wall of the first cavity 101 and thus preventing the formation of aerosols.

[0128] like Figure 13 As shown, in some embodiments, the depth of the first inclined surface 1012 is 0.2 to 0.3 times the total depth of the first cavity 101. The depth of the first inclined surface 1012 refers to the height of the first inclined surface 1012 along the first central axis L1, and the total depth of the first cavity 101 refers to the total height of the first cavity 101 along the first central axis L1.

[0129] like Figure 13 As shown, in one embodiment, the sidewall of the sixth cavity 106 is provided with a second flow guiding structure 1061, which extends from the opening of the sixth cavity 106 towards the bottom of the sixth cavity 106. In this embodiment, by providing the second flow guiding structure 1061 on the sidewall of the sixth cavity 106 to guide the liquid injected into the sixth cavity 106, the liquid injected into the sixth cavity 106 can be prevented from churning, thereby avoiding the formation of aerosols and reducing the risk of infection for testing personnel.

[0130] like Figure 13 As shown, in some embodiments, the sixth cavity 106 has a second central axis L2, and the second flow guiding structure 1061 includes a second inclined surface 1062, which extends obliquely from the edge of the opening in the sixth cavity 106 toward the second central axis L2. In actual use, the waste liquid injected into the sixth cavity 106 is directly discharged onto the second inclined surface 1062, and then flows to the bottom of the sixth cavity 106 under the guidance of the second inclined surface 1062.

[0131] It should be noted that the second inclined surface 1062 is not limited to being located at the opening edge of the sixth cavity 106, but can also be located in the middle or bottom of the sixth cavity 106.

[0132] It should also be noted that the second flow guiding structure 1061 is not limited to being configured as the second inclined surface 1062. For example, in some other embodiments, the second flow guiding structure 1061 includes a first helical surface that extends spirally from the edge of the opening of the sixth cavity 106 around the second central axis L2 toward the bottom of the sixth cavity 106.

[0133] As shown in FIG. 6, in some embodiments, the second inclined surface 1062 has an inclination angle of β1 with respect to the second central axis L2, and β1 = 5°-45°. For example, the second inclined surface 1062 has an inclination angle of 5°, 10°, 15°, 20°, 25°, 30°, 35°, 40°, 45°, or any value between any two adjacent values among 5°, 10°, 15°, 20°, 25°, 30°, 35°, 40°, 45°. In this embodiment, the second inclined surface 1062 has an inclination angle of 6.8° with respect to the second central axis L2. Figure 13

[0134] As shown in FIG. 6, in some embodiments, the side wall of the sixth cavity 106 further comprises a third wall surface 1063 extending from the bottom end of the second inclined surface 1062 to the bottom of the sixth cavity 106, and the third wall surface 1063 has an inclination angle of β2 with respect to the second central axis L2, and β2 = 0°-2°. Figure 9

[0135] As shown in FIG. 6, in some embodiments, the second inclined surface 1062 has a depth of 0.3-0.5 of the total depth of the sixth cavity 106. The depth of the second inclined surface 1062 refers to the height of the second inclined surface 1062 along the direction of the second central axis L2, and the total depth of the sixth cavity 106 refers to the total height of the sixth cavity 106 along the direction of the second central axis L2. Figure 14

[0136] As shown in FIG. 1, in one embodiment, the extraction member 100 comprises a strip member 11 and at least two tube bodies 12, the strip member 11 comprises a first side 11a and a second side 11b opposite to the first side 11a in the thickness direction X of the strip member 11, and the strip member 11 is provided with at least two openings arranged along the length direction of the strip member 11 and penetrating through the strip member 11 in the thickness direction X. The at least two tube bodies 12 are arranged on the second side 11b of the strip member 11, and the at least two tube bodies 12 are in one-to-one correspondence with the at least two openings, wherein the inner cavity of one of the tube bodies 12 forms the first cavity 101, and the inner cavity of the other tube body 12 forms the second cavity 102. Figure 15 Figure 15 As shown in FIG. 1, in one embodiment, the strip member 11 comprises a first end surface 11c and a second end surface 11d opposite to the first end surface 11c in the length direction Y of the strip member 11, the first end surface 11c is recessed towards the second end surface 11d to form a first positioning groove 111, and the second end surface 11d is recessed towards the first end surface 11c to form a second positioning groove 112. The first positioning groove 111 and the second positioning groove 112 are used to position the extraction member 100 when the clamping jaws of the scheduling assembly 208 clamp the extraction member 100.

[0137] As shown in FIG. 1, in one embodiment, the strip member 11 comprises a first end surface 11c and a second end surface 11d opposite to the first end surface 11c in the length direction Y of the strip member 11, the first end surface 11c is recessed towards the second end surface 11d to form a first positioning groove 111, and the second end surface 11d is recessed towards the first end surface 11c to form a second positioning groove 112. The first positioning groove 111 and the second positioning groove 112 are used to position the extraction member 100 when the clamping jaws of the scheduling assembly 208 clamp the extraction member 100. Figure 15 As shown in FIG. 1, in one embodiment, the strip member 11 comprises a first end surface 11c and a second end surface 11d opposite to the first end surface 11c in the length direction Y of the strip member 11, the first end surface 11c is recessed towards the second end surface 11d to form a first positioning groove 111, and the second end surface 11d is recessed towards the first end surface 11c to form a second positioning groove 112. The first positioning groove 111 and the second positioning groove 112 are used to position the extraction member 100 when the clamping jaws of the scheduling assembly 208 clamp the extraction member 100.​​​​

[0138] In the actual sample detection process, the clamping jaw needs to clamp the extraction piece 100 to take and place in multiple stations. If the clamping jaw clamps the extraction piece 100 with an error, the smooth taking and placing of the extraction piece 100 in multiple stations cannot be achieved. In the embodiment, the first positioning groove 111 is formed by recessing the first end surface 11c towards the second end surface 11d, and the second positioning groove 112 is formed by recessing the second end surface 11d towards the first end surface 11c. When the clamping jaw clamps the extraction piece 100, the extraction piece 100 can be positioned, so that the extraction piece 100 can be smoothly taken and placed in multiple stations.

[0139] As shown in Figure 15 , in one embodiment, the width of the first positioning groove 111 gradually increases from the bottom of the first positioning groove 111 towards the slot opening direction; the width of the second positioning groove 112 gradually increases from the bottom of the second positioning groove 112 towards the slot opening direction.

[0140] As shown in Figure 16 , in one embodiment, the shapes of the first positioning groove 111 and the second positioning groove 112 are V-shaped. Correspondingly, a V-shaped protrusion is provided on the clamping jaw. In this implementation, when the protrusion on the clamping jaw engages with the first positioning groove 111 and the second positioning groove 112, the side surface of the protrusion can tightly fit the wall surface of the first positioning groove 111 and the second positioning groove 112, so as to form accurate positioning. Moreover, since the first positioning groove 111 and the second positioning groove 112 have large openings and the protrusion has a small tip profile, when the clamping jaw clamps the extraction piece 100, it is very convenient for the protrusion to be embedded into the first positioning groove 111 and the second positioning groove 112, thereby reducing the clamping difficulty.

[0141] As shown in Figure 14 and Figures 17 to 19 , in one embodiment, the strip-shaped piece 11 includes a third side surface 11e and a fourth side surface 11f opposite to the third side surface 11e in the width direction Z, one of the third side surface 11e and the fourth side surface 11f is provided with a protrusion 113, and the other of the third side surface 11e and the fourth side surface 11f is provided with a clamping groove 114, the protrusion 113 and the clamping groove 114 are opposite in the width direction Z and are complementary in shape. The two extraction pieces 100 are positioned through the cooperation of the protrusion 113 and the clamping groove 114. In this implementation, when the two extraction pieces 100 are stacked in the width direction Z of the strip-shaped piece 11, the protrusion 113 of one extraction piece 100 can be clamped with the clamping groove 114 of the other extraction piece 100, so as to limit the sliding of the two extraction pieces 100 in the length direction Y. This is not only conducive to the packaging of the row of extraction pieces 100, but also conducive to the entry of the row of extraction pieces 100 into the sample detection device 1000.

[0142] As shown in Figure 3As shown, in one embodiment, the first side 11a of the strip-shaped member 11 is provided with a groove 115, and the projection of the groove 115 and the opening on the plane perpendicular to the thickness direction X of the strip-shaped member 11 does not overlap. With this implementation, by providing the groove 115 on the first side 11a of the strip-shaped member 11, the hanging liquid of the first pipetting head 21 and the second pipetting head 22 can fall into the groove 115 after falling, and the groove 115 can hold the hanging liquid, avoiding the hanging liquid from dripping from the extraction member to the work station again, thereby keeping the work station clean and reducing the pollution of the work station.

[0143] As shown, Figure 9 As shown, in one embodiment, the number of openings is six, which are a first opening, a second opening, a third opening, a fourth opening, a fifth opening and a sixth opening arranged in sequence. The number of tube bodies 12 is six, which are a first tube body 12a, a second tube body 12b, a third tube body 12c, a fourth tube body 12d, a fifth tube body 12e and a sixth tube body 12f arranged in sequence, the first tube body 12a to the sixth tube body 12f correspond to the first opening to the sixth opening in communication one by one, the inner cavity of the first tube body 12a forms a first cavity 101, and the inner cavity of one of the second tube body 12b and the third tube body 12c forms a second cavity 102. The length of the first tube body 12a is greater than the length of the second tube body 12b and the length of the third tube body 12c.

[0144] As shown, Figure 14 As shown, in one embodiment, the sum of the length of the first tube body 12a and the length of the sixth tube body 12f is t1, the sum of the length of the second tube body 12b and the length of the fifth tube body 12e is t2, and the sum of the length of the third tube body 12c and the length of the fourth tube body 12d is t3, wherein t1≥t2, and t1≥t3. The first tube body 12a includes a first tube segment 12a1 and a second tube segment 12a2, the first tube segment 12a1 includes a first end and a second end, the first end of the first tube segment 12a1 is connected with the strip-shaped member 11, the second tube segment 12a2 is connected with the second end of the first tube segment 12a1, the fourth tube body 12d includes a third tube segment 12d1 and a fourth tube segment 12d2, the third tube segment 12d1 includes a first end and a second end, the first end of the third tube segment 12d1 is connected with the strip-shaped member 11, and the fourth tube segment 12d2 is connected with the second end of the third tube segment 12d1, wherein the spacing between the second tube segment 12a2 and the fourth tube segment 12d2 is S1, and the spacing between the two sides of the fourth tube segment 12d2 and the fifth tube body 12e is S2, S1>S2. With this implementation, the space between the first tube body 12a and the fourth tube body 12d can be reasonably utilized, and the two extraction members 100 can be placed in reverse and crossed, and the occupation of the extraction member 100 not only facilitates packaging, but also facilitates transportation.

[0145] Specifically, the length of the first tube body 12a is M1, the length of the sixth tube body 12f is M2, M1+M2=t1, the length of the second tube body 12b is M3, the length of the fifth tube body 12e is M4, M3+M4=t2, the length of the third tube body 12c is M5, the length of the fourth tube body 12d is M6, M5+M6=t3.

[0146] As shown in Figure 15 , ​ , ​ and ​ The embodiment of the utility model further proposes an extracting piece 100, the extracting piece 100 that proposes includes strip-shaped piece 11 and at least two tube bodies 12, including first side 11a and second side 11b opposite first side 11a in the thickness direction X of strip-shaped piece 11, and strip-shaped piece 11 is equipped with at least two openings, at least two openings are arranged along the length direction Y of strip-shaped piece 11, and in the thickness direction X, at least two tube bodies 12 are arranged in the second side 11b of strip-shaped piece 11, and at least two tube bodies 12 are communicated with at least two openings one by one. Wherein, strip-shaped piece 11 includes first end surface 11c and opposite second end surface 11d in the length direction Y, first end surface 11c is recessed to form first positioning groove 111 towards second end surface 11d, and second end surface 11d is recessed to form second positioning groove 112 towards first end surface 11c.

[0147] The extracting piece 100 proposed in the embodiment, by recessing first end surface 11c of strip-shaped piece 11 to form first positioning groove 111 towards second end surface 11d, and recessing second end surface 11d to form second positioning groove 112 towards first end surface 11c, when the clamping jaw clamps the extracting piece 100, the clamping jaw can form positioning to the extracting piece 100, so that the extracting piece 100 can be smoothly taken and placed in multiple stations.

[0148] In one embodiment, the width of the first positioning groove 111 gradually increases from the bottom of the first positioning groove 111 towards the slot opening direction. The width of the second positioning groove 112 gradually increases from the bottom of the second positioning groove 112 towards the slot opening direction.

[0149] In one embodiment, the first positioning groove 111 and the second positioning groove 112 are V-shaped. Correspondingly, the clamping jaw is provided with a V-shaped protrusion 113. In this embodiment, when the protrusion 113 on the clamping jaw engages with the first positioning groove 111 and the second positioning groove 112, the side surface of the protrusion 113 can tightly fit the wall surface of the first positioning groove 111 and the second positioning groove 112, so that accurate positioning can be formed. Moreover, since the opening of the first positioning groove 111 and the second positioning groove 112 is large and the tip profile of the protrusion 113 is small, when the clamping jaw clamps the extracting piece 100, it is very convenient for the protrusion 113 to be embedded into the first positioning groove 111 and the second positioning groove 112, thereby reducing the clamping difficulty.

[0150] The other structure and beneficial effects of the extraction part 100 proposed in the embodiment can refer to the above-mentioned embodiments, and will not be described here.

[0151] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited to this, any skilled person in the art can easily think of various equivalent modifications or replacements within the technical range disclosed by the present application, and these modifications or replacements should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. An extraction piece applied to a molecular diagnostic device, wherein a reagent dispensing assembly and a sample dispensing assembly are provided in a housing of the molecular diagnostic device, characterized in that, The extraction piece comprises an open first cavity and a second cavity, the first cavity is used for accommodating the first reagent injected by the reagent dispensing assembly and the sample injected by the sample dispensing assembly, and the second cavity is used for accommodating the second reagent injected by the reagent dispensing assembly.

2. The extraction member of claim 1, wherein, The extraction piece further comprises an open third cavity and a fourth cavity, the third cavity is used for accommodating a first pipette, and the fourth cavity is used for accommodating a second pipette, the volume of the second pipette is greater than that of the first pipette; and / or, The extraction piece further comprises an open fifth cavity, and the fifth cavity is used for accommodating a third reagent injected by the molecular diagnostic device; and / or, The extraction piece further comprises an open sixth cavity, and the sixth cavity is used for accommodating waste liquid.

3. The extraction member of claim 2, wherein, The extraction piece further comprises a first pipette and a second pipette, the first pipette is accommodated in the third cavity, and the second pipette is accommodated in the fourth cavity.

4. The extraction member of claim 2, wherein, A first step or a first stopper is arranged on the side wall of the third cavity and close to the opening of the third cavity; and / or, A second step or a second stopper is arranged on the side wall of the fifth cavity and close to the opening of the fifth cavity; and / or, A first protrusion is arranged on the bottom of the third cavity; and / or, A second protrusion is arranged on the bottom of the fourth cavity.

5. The extraction member of claim 1, wherein, A first flow guide structure is arranged on the side wall of the first cavity, and the first flow guide structure extends from the opening of the first cavity to the bottom of the first cavity.

6. The extraction member of claim 2, wherein, A second flow guide structure is arranged on the side wall of the sixth cavity, and the second flow guide structure extends from the opening of the sixth cavity to the bottom of the sixth cavity.

7. The extraction member of claim 1, wherein It comprises: A strip-shaped piece comprising a first side and a second side opposite to the first side in the thickness direction of the strip-shaped piece, the strip-shaped piece is provided with at least two openings, the at least two openings are arranged along the length direction of the strip-shaped piece and penetrate through the strip-shaped piece in the thickness direction; At least two tubes are arranged on the second side of the strip-shaped piece, the at least two tubes communicate with the at least two openings one by one, and the inner cavity of one of the tubes forms the first cavity, and the inner cavity of another of the tubes forms the second cavity.

8. The extraction member of claim 7, wherein, The strip-shaped piece comprises a first end face and a second end face opposite to the first end face in the length direction, the first end face is recessed towards the second end face to form a first positioning groove, and the second end face is recessed towards the first end face to form a second positioning groove.

9. The extraction member of claim 8, wherein, The width of the first positioning groove gradually increases from the bottom of the first positioning groove to the opening direction, and the width of the second positioning groove gradually increases from the bottom of the second positioning groove to the opening direction.

10. The extraction member of claim 9, wherein, The shapes of the first positioning groove and the second positioning groove are V-shaped.

11. The extraction member of claim 7, wherein, The strip-shaped piece comprises a third side and a fourth side opposite to the third side in the width direction, one of the third side and the fourth side is provided with a protrusion, and the other of the third side and the fourth side is provided with a clamping groove, the protrusion and the clamping groove are opposite to each other in the width direction and are complementary in shape.

12. The extraction member of claim 7, wherein, The first side of the strip-shaped piece is provided with a groove, and the projection of the groove and the opening on a plane perpendicular to the thickness direction of the strip-shaped piece does not overlap.

13. The extraction member of claim 7, wherein, The number of the openings is six, which are a first opening, a second opening, a third opening, a fourth opening, a fifth opening and a sixth opening arranged in sequence; The number of the tube bodies is six, which are a first tube body, a second tube body, a third tube body, a fourth tube body, a fifth tube body and a sixth tube body arranged in sequence, the first tube body to the sixth tube body correspond to the first opening to the sixth opening in communication, the inner cavity of the first tube body forms the first cavity, the inner cavity of one of the second tube body and the third tube body forms the second cavity; The length of the first tube body is greater than the length of the second tube body and the length of the third tube body.

14. The extraction member of claim 13, wherein, The sum of the length of the first tube body and the length of the sixth tube body is t1, the sum of the length of the second tube body and the length of the fifth tube body is t2, and the sum of the length of the third tube body and the length of the fourth tube body is t3, wherein t1≥t2 and t1≥t3; The first tube body comprises a first tube segment and a second tube segment, the first tube segment comprises a first end and a second end, the first end of the first tube segment is connected with the strip-shaped member, the second tube segment is connected with the second end of the first tube segment, the fourth tube body comprises a third tube segment and a fourth tube segment, the third tube segment comprises a first end and a second end, the first end of the third tube segment is connected with the strip-shaped member, the fourth tube segment is connected with the second end of the third tube segment, wherein the distance between the second tube segment and the fourth tube segment is S1, the distance between the fourth tube segment and the fifth tube body on the opposite sides is S2, and S1>S2.

15. An extraction member characterized by, Comprise: A strip-shaped member comprising a first side and a second side opposite to the first side in the thickness direction of the strip-shaped member, the strip-shaped member is provided with at least two openings, the at least two openings are arranged along the length direction of the strip-shaped member and penetrate through the strip-shaped member in the thickness direction; At least two tube bodies provided on the second side of the strip-shaped member, the at least two tube bodies correspond to the at least two openings in communication; Wherein, the strip-shaped member comprises a first end face and a second end face opposite to the first end face in the length direction, the first end face is recessed to form a first positioning groove towards the second end face, and the second end face is recessed to form a second positioning groove towards the first end face.

16. The extraction member of claim 15, wherein, The width of the first positioning groove gradually increases from the bottom of the first positioning groove towards the opening direction; the width of the second positioning groove gradually increases from the bottom of the second positioning groove towards the opening direction.

17. The extraction member of claim 16, wherein, The shapes of the first positioning groove and the second positioning groove are V-shaped.

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