Detection device

Abstract

The utility model relates to the technical field of temperature control, in particular to detection equipment. The utility model aims to solve the problem that the result accuracy and uniformity are influenced due to non-uniform temperature distribution on an existing metal module. The detection equipment comprises at least one heating module, the heating module comprises a first heater and a plurality of second heaters, and the first heater is arranged on a bottom plate of an incubation seat and deviates from a reactor of the incubation seat; the plurality of second heaters are arranged on the incubation seat and are respectively used for heating the plurality of peripheral reactors; the heating power of the second heater is higher than the heating power of the first heater, and the second heater is used for carrying out compensation heating on the peripheral reactors, so that the heating uniformity of the heating module on the plurality of reactors is improved, and the accuracy and uniformity of experiments are improved.

Description

Technical Field

[0001] This application relates to the field of temperature control technology, and more particularly to a detection device. Background Technology

[0002] Gene amplification instruments are fundamental instruments in the biomedical field used for polymerase chain reaction (PCR). They provide a stable and adjustable temperature environment and are widely used in the industry for DNA fragment amplification. The working principle of a gene amplification instrument is to complete a process similar to the natural replication process of DNA—denaturation, annealing, and extension—through three basic reaction steps. Accurate temperature control is crucial to ensuring the accuracy and reliability of DNA amplification.

[0003] In related technologies, a PCR heating module includes a metal module, a semiconductor cooler, and a heat sink. The semiconductor cooler is located between the metal module and the heat sink. Multiple sample wells are formed on the metal module for placing sample tubes. The semiconductor cooler is used to heat and cool the metal module and sample tubes, while the heat sink dissipates heat from the semiconductor cooler.

[0004] However, the uneven temperature distribution on the existing metal module leads to uneven temperatures in multiple sample tubes, resulting in inconsistent DNA fragment amplification efficiency and thus affecting the accuracy and consistency of experimental results. Utility Model Content

[0005] This application provides a testing device to solve the technical problem of uneven temperature distribution on existing metal modules, which affects the accuracy and uniformity of results.

[0006] To solve the above-mentioned technical problems, this application adopts the following technical solution:

[0007] This application provides a testing device, including at least one heating module; the heating module includes a control unit, an incubation base, a first heater, and a plurality of second heaters;

[0008] The control unit is electrically connected to the first heater and the plurality of second heaters respectively;

[0009] The incubation base includes a base plate and a plurality of reactors disposed on the base plate, the reactors being used to hold sample containers;

[0010] The first heater is located on the side of the bottom plate opposite to the reactor;

[0011] The plurality of second heaters are arranged on the incubation seat and are respectively used for heating a plurality of peripheral reactors, at least one of the plurality of second heaters is independently controlled, and the heating power of the second heater is lower than the heating power of the first heater.

[0012] Compared with the prior art, the detection device provided by the first aspect of the present application has the following advantages:

[0013] The detection device provided by the present application comprises at least one heating module; the heating module comprises a first heater and a plurality of second heaters, the first heater is arranged on the bottom plate of the incubation seat and faces away from the reactors of the incubation seat; and the plurality of second heaters are arranged on the incubation seat and are respectively used for heating a plurality of peripheral reactors. The heating power of the second heater is higher than the heating power of the first heater, and the peripheral reactors are compensated and heated by the second heater, so as to improve the uniformity of the heating module in heating the plurality of reactors, thereby improving the accuracy and uniformity of the experiment.

[0014] As an improvement of the above-mentioned detection device of the present application, each of the plurality of second heaters is independently controlled.

[0015] As an improvement of the above-mentioned detection device of the present application, at least one of the second heaters is arranged on the bottom plate.

[0016] As an improvement of the above-mentioned detection device of the present application, at least one of the second heaters is provided with at least one through hole, so that the second heater is sleeved outside one of the peripheral reactors.

[0017] As an improvement of the above-mentioned detection device of the present application, at least one of the second heaters is arranged on the side wall of the reactor.

[0018] As an improvement of the above-mentioned detection device of the present application, the incubation seat has a first center line, the plurality of reactors are symmetrically arranged about the first center line, and the plurality of reactors are symmetrically arranged about the second center line; and the plurality of second heaters are symmetrically arranged about the first center line.

[0019] As an improvement of the above-mentioned detection device of the present application, the incubation seat further has a second center line, the second center line is perpendicular to the first center line; the plurality of reactors are symmetrically arranged about the second center line; and the plurality of second heaters are symmetrically arranged about the second center line.

[0020] As an improvement of the above-mentioned detection device of the present application, the plurality of reactors are arranged in a rectangular matrix on the bottom plate; and four second heaters are respectively used for heating the reactors at the four top corners.

[0021] As an improvement of the detection device described above, the first heater is provided with one.

[0022] As an improvement of the detection device described above, the first heater is provided with a plurality of first heaters, and the plurality of first heaters are arranged in a rectangular matrix on the side of the bottom plate facing away from the reactor.

[0023] As an improvement of the detection device described above, when the first heater is provided with a plurality of first heaters, at least one second heater is arranged at the adjacent position of two adjacent first heaters.

[0024] As an improvement of the detection device described above, the second heater is in the form of a sheet.

[0025] As an improvement of the detection device described above, the heating module comprises a heat preservation structure arranged above the incubation seat.

[0026] As an improvement of the detection device described above, a heat spreading sheet is arranged between the first heater and the bottom plate, the heat spreading sheet is configured to have a first thermal conductivity on the plate surface of the bottom plate, and the heat spreading sheet has a second thermal conductivity in the direction perpendicular to the plate surface of the bottom plate, the first thermal conductivity is greater than the second thermal conductivity.

[0027] As an improvement of the detection device described above, the heating module further comprises a heat sink, the heat sink is in contact with the side of the first heater facing away from the incubation seat; a heat conducting gasket is arranged between the heat sink and the first heater.

[0028] In addition to the technical problems solved by the present application, the technical features constituting the technical solutions and the beneficial effects brought by these technical features described above, other technical problems solved by the detection device provided by the present application, other technical features included in the technical solutions and the beneficial effects brought by these technical features will be further described in detail in the specific embodiments. BRIEF DESCRIPTION OF DRAWINGS

[0029] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the description of the embodiments of the present application or the prior art will be briefly introduced. Obviously, the drawings in the following description are only a part of the embodiments of the present application, and these drawings and the written description are not intended to limit the scope of the present application in any way, but to illustrate the concept of the present application to those skilled in the art by reference to specific embodiments. Those skilled in the art can obtain other drawings without creative labor on the basis of these drawings.

[0030] Figure 1 A structural schematic diagram of a heating module provided for an embodiment of the present application;

[0031] Figure 2 An exploded view of a heating module provided for an embodiment of the present application;

[0032] Figure 3 A top view of an incubation seat and a second heater provided for an embodiment of the present application;

[0033] Figure 4 A schematic diagram of an incubation seat and a second heater provided for an embodiment of the present application;

[0034] Figure 5 A temperature field distribution schematic diagram of an incubation seat provided for an embodiment of the present application;

[0035] Figure 6 A position schematic diagram of an incubation seat provided for an embodiment of the present application which needs temperature compensation;

[0036] Figure 7 A schematic diagram of an incubation seat and a second heater provided for another embodiment of the present application;

[0037] Figure 8 A schematic diagram of an incubation seat and a second heater provided for yet another embodiment of the present application;

[0038] Figure 9 A schematic diagram of an incubation seat and a second heater provided for yet another embodiment of the present application;

[0039] Figure 10 A schematic diagram of an incubation seat and a second heater provided for yet another embodiment of the present application;

[0040] Figure 11 A schematic diagram of an incubation seat and a second heater provided for yet another embodiment of the present application.

[0041] Legend of reference signs:

[0042] 10: heating module;

[0043] 100: incubation seat; 110: bottom plate; 120: reactor; 130: plastic screw;

[0044] 200: first heater;

[0045] 300: second heater; 301: through hole;

[0046] 400: heat preservation structure;

[0047] 510: heat spreading sheet; 520: heat sink; 530: heat conductive gasket. DETAILED DESCRIPTION

[0048] Embodiments of the present application are described below in detail, examples of which are shown in the drawings, wherein the same or similar notations represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by reference to the drawings are exemplary and are intended to explain the present application, and cannot be understood as limiting the present application.

[0049] In combination Figure 1 and Figure 2 Embodiments of the present application provide a detection device, which can perform amplification operation on a sample containing target DNA.

[0050] The detection device can include a housing, and at least one heating module 10 disposed in the housing, the heating module 10 can heat the sample in the sample container to complete the thermal cycle process of the sample. In some implementations, the detection device can include multiple heating modules 10 to increase the amount of samples processed simultaneously.

[0051] In some embodiments, the housing can further include a detection module and a scheduling mechanism, etc. The scheduling mechanism can transfer the sample container to the heating module 10 for thermal cycle operation, and after the thermal cycle operation is completed, the sample container is transferred to the detection module for detection to obtain the detection result.

[0052] Wherein, the detection device can be a gene amplification instrument or an amplification analyzer, etc. Embodiments of the present application do not limit this.

[0053] The heating module 10 includes a control unit, an incubation seat 100, a first heater 200 and a plurality of second heaters 300. Wherein, the sample container can be placed on the incubation seat 100, and the control unit is used to control the heating temperature of the incubation seat 100 by the first heater 200 and the second heater 300, thereby controlling the heating temperature of the sample in the sample container.

[0054] The control unit is used to control the heating state of the heating module 10. Specifically, the control unit is electrically connected with the first heater 200 and the plurality of second heaters 300 respectively, and the control unit is configured to control the heating power of the first heater 200 and the heating power of the second heater 300, thereby controlling the heating temperature of the incubation seat 100 by the first heater 200 and the second heater 300.

[0055] The incubation seat includes a bottom plate 110 and a plurality of reactors 120 disposed on the bottom plate 110. The reactor 120 is configured to form an open-top receiving groove for receiving the sample container. The sample container is used to contain the sample, and the sample container can be a sample tube, for example.

[0056] In some embodiments, the incubation seat 100 can be a metal seat, which can improve the heat transfer efficiency.

[0057] Multiple reactors 120 are arranged in a rectangular matrix on the base plate 110. For example, sample containers are typically eight-tube arrays. Therefore, the number of reactors 120 in a single row and / or column on the base plate 110 is a multiple of eight to facilitate the placement of sample containers.

[0058] The first heater can be an electric heater, or the first heater 200 can be a semiconductor heater, etc. In some embodiments of this application, the first heater 200 is a semiconductor heater, which can heat up and cool down quickly, and the temperature control of the semiconductor heater is more precise, which is crucial for the efficient execution of the PCR reaction.

[0059] In some embodiments, the first heater 200 is a plate heater, which can have a large contact area, improve the uniformity of heating multiple reactors 120, and also help reduce the overall volume of the heating module 10.

[0060] The first heater 200 is located on the side of the bottom plate 110 opposite to the reactor 120. Figure 2 In the direction shown, a reactor 120 is disposed on the top surface of the base plate 110, and a first heater 200 is disposed on the bottom surface of the base plate 110.

[0061] In some embodiments, a heat spreader 510 is provided between the first heater 200 and the base plate 110. The heat spreader 510 is configured to have a first thermal conductivity on the surface of the base plate 110 and a second thermal conductivity in a direction perpendicular to the surface of the base plate 110. The first thermal conductivity is greater than the second thermal conductivity.

[0062] exist Figure 2 In the directions shown, the surface of the base plate 110 is parallel to the XY plane, and the direction perpendicular to the surface of the base plate 110 is... Figure 2 In the Z-axis direction, the thermal conductivity of the heat spreader 510 in the plane parallel to the XY plane is greater than its thermal conductivity in the Z-axis direction.

[0063] For example, the heat spreader 510 can be anisotropic materials such as graphene sheets or graphite sheets.

[0064] In this embodiment, by setting a heat spreader 510 as a thermal interface material, the temperature uniformity within the plate surface of the base plate 110 is improved, which is beneficial to improving the accuracy of the PCR reaction.

[0065] Continue to refer to Figure 2 In this embodiment, the first heater 200 is a semiconductor heater, which has a cold end and a hot end. While the hot end is heating, the temperature of the cold end decreases. If the heat dissipation of either the cold end or the hot end is not timely, it will affect the heat of the other end.

[0066] To this end, in some embodiments, the heating module 10 further comprises a heat sink 520, which is in contact with the side of the first heater 200 away from the incubation seat 100. The heat sink 520 is configured to dissipate the heat released by the first heater 200 during operation.

[0067] In some embodiments, a heat-conducting gasket 530 is arranged between the heat sink 520 and the first heater 200 to reduce the heat transfer resistance between the first heater 200 and the first heat sink 520 and improve the heat dissipation efficiency of the heat sink 520.

[0068] Continuing to refer to Figure 2 , the incubation seat 100 can be fixed to the heat sink 520, and the first heater 200, the heat spreader 510, and the heat-conducting gasket 530 are sandwiched between the bottom plate 110 of the incubation seat 100 and the heat sink 520. The heat spreader 510, the first heater 200, and the heat-conducting gasket 530 are arranged in a direction from top to bottom (corresponding to the direction from the positive direction of the Z-axis to the negative direction in Figure 2 ).

[0069] In some embodiments, the bottom plate 110 of the incubation seat 100 and the heat sink 520 can be fixedly connected by fasteners, and the connection is stable and reliable.

[0070] For example, the bottom plate 110 and the heat sink 520 are fixedly connected by plastic screws 130. The thermal resistance of the plastic screws 130 is large, which can reduce the heat loss of the incubation seat 100, thereby improving the temperature uniformity of the incubation seat 100.

[0071] Continuing to refer to Figure 2 , the plurality of second heaters 300 of the embodiments of the present application are arranged on the incubation seat 100 for heating the incubation seat 100.

[0072] The second heater 300 can be an electric heater, which can be an electric heating sheet, a heating resistor, or a resistance wire, etc.

[0073] The second heater 300 can be in the form of a sheet, which not only has a large heating area, but also occupies a small space during installation.

[0074] The heating power of the second heater 300 is lower than that of the first heater 200, the first heater 200 functions as the main heating, and the second heater 300 functions as the auxiliary heating. Thus, the first heater 200 and the second heater 300 jointly heat the incubation seat 100, so that the temperature of each reactor on the incubation seat 100 is more uniform, thereby improving the accuracy and uniformity of the experimental structure.

[0075] In some embodiments, the at least one second heater 300 is disposed on the bottom plate 110. In this way, the surface of the bottom plate 110 is flat, which not only provides sufficient installation space for the second heater 300, but also simplifies the installation method.

[0076] In an exemplary embodiment, the at least one second heater 300 is disposed on the side of the bottom plate 110 facing the reactor 120. As shown in Figure 2 In an exemplary embodiment, the at least one second heater 300 is disposed on the top surface of the bottom plate 110.

[0077] In some possible implementations, the at least one second heater 300 is in the form of a strip, so that the second heater 300 can extend in the space between the reactors 120, which is simple in structure and facilitates processing and installation.

[0078] In another possible implementation, the at least one second heater 300 is in the form of an arc, so that the second heater 300 can be arranged on the outside of at least part of the reactors 120. In this way, not only a larger heating area can be provided, but also the installation and positioning of the second heater 300 are facilitated.

[0079] In yet another possible implementation, the at least one second heater 300 is provided with at least one through hole 301, so that the second heater 300 is arranged outside one of the peripheral reactors 120. In this way, not only is the second heater 300 easy to position and install, but also the temperature compensation of the second heater 300 for the peripheral reactor 120 is improved.

[0080] Generally, the reactor 120 is in the form of a cylinder, and the through hole 301 is a circular hole, so that the shape of the through hole 301 matches the shape of the reactor 120, which facilitates the uniformity of heat transfer in the circumferential direction of the reactor 120.

[0081] In an exemplary embodiment, the at least one second heater 300 is disposed on the side of the bottom plate 110 facing the reactor 120. As shown in Figure 3 In an exemplary embodiment, the at least one second heater 300 is disposed on the top surface of the bottom plate 110.

[0082] In another embodiment, the at least one second heater 300 is disposed on the side wall of the reactor 120. In an exemplary embodiment, the at least one second heater 300 is bonded to the side wall of the reactor 120. In this way, the second heater 300 can directly heat the sample container in the reactor 120, which facilitates the efficiency of heat transfer to the sample container.

[0083] The installation positions of the plurality of second heaters 300 can be the same, for example, all the second heaters 300 are installed on the top surface of the bottom plate 110; for another example, all the second heaters 300 are installed on the bottom surface of the bottom plate 110; for yet another example, all the second heaters 300 are installed on the side wall of the reactor 120.

[0084] The installation positions of the plurality of second heaters 300 can be different, for example, at least one second heater 300 is arranged on the top surface of the bottom plate 110; for another example, at least one second heater 300 is arranged on the bottom surface of the bottom plate 110; for yet another example, at least one second heater 300 is arranged on the side wall of the reactor 120.

[0085] In some specific implementations of the present application, all the second heaters 300 are installed on the top surface of the bottom plate 110, so that the heating efficiency of the second heaters 300 on the sample containers in the reactor 120 can be ensured, and the installation mode of the second heaters 300 is simple, and the wiring arrangement of the plurality of second heaters 300 is facilitated.

[0086] In combination Figure 3 , the plurality of second heaters 300 of the embodiment of the present application are respectively used to heat the plurality of peripheral reactors 120.

[0087] In this way, the heating module 10 of the embodiment of the present application includes the first heater 200 and the plurality of second heaters 300, the first heater 200 is arranged on the bottom plate 110 of the incubator seat 100 and faces away from the reactor 120 of the incubator seat 100; the plurality of second heaters 300 are arranged on the incubator seat 100 and are respectively used to heat the plurality of peripheral reactors 120. The heating power of the second heater 300 is lower than the heating power of the first heater 200, and the peripheral reactor 120 is compensated and heated by the second heater 300, so as to improve the uniformity of the heating of the plurality of reactors 120 by the heating module 10, and to improve the accuracy and consistency of the experiment.

[0088] Moreover, the embodiment of the present application compensates the temperature of the low-temperature area at the edge by increasing the second heater 300, uses lower cost and simple process, and realizes higher precision temperature compensation.

[0089] The plurality of reactors 120 of the incubator seat 100 are arranged in a rectangular matrix on the bottom plate 110, and the peripheral reactor 120 refers to the reactor 120 close to the edge of the bottom plate 110. For example, as shown in the middle Figure 3 For example, 32 reactors 120 are arranged in a 4x8 rectangular matrix, wherein 8 reactors 120 are arranged at intervals along the X-axis direction to form a column, and 4 reactors 120 are arranged at intervals along the Y-axis direction to form a row.

[0090] The peripheral reactors 120 include: ① eight reactors 120 arranged in a row close to the left edge of the bottom plate 110; ② eight reactors 120 arranged in a row close to the right edge of the bottom plate 110; ③ two reactors 120 arranged in a row close to the top edge of the bottom plate 110; and ④ two reactors 120 arranged in a row close to the bottom edge of the bottom plate 110.

[0091] In Figure 2 In some embodiments, the left-to-right direction is parallel to the Y-axis direction, and the top-to-bottom direction is parallel to the X-axis direction.

[0092] It should be noted that the plurality of second heaters 300 are used to heat the plurality of peripheral reactors 120, and not necessarily all of the peripheral reactors 120. That is, the plurality of second heaters 300 are used to heat at least part of the plurality of peripheral reactors 120.

[0093] Continuing to refer to Figure 4 In some embodiments, the heating module 10 includes a heat preservation structure 400 arranged above the incubation seat 100 to improve the heat preservation performance of the incubation seat 100.

[0094] The heat preservation structure 400 can include a heat preservation plate, which can be heat preservation foam. The heat preservation plate is provided with an avoiding opening to avoid the sample container. In this way, at least part of the sample container can be exposed outside the heat preservation plate, facilitating experimental operation.

[0095] When the second heater 300 is arranged on the top surface of the bottom plate 110, the heat preservation plate can also be configured to press the second heater 300 to ensure the tightness of the contact between the second heater 300 and the bottom plate 110, facilitating heat transfer.

[0096] The heat preservation structure 400 can include a heat preservation mask arranged above the heat preservation plate. The heat preservation mask is provided with an avoiding hole to expose at least part of the sample container through the avoiding hole, facilitating operation.

[0097] In some embodiments, the heat preservation mask is fixed on the heat sink 520 by a screw to clamp the heat preservation plate between the heat preservation mask and the incubation seat 100, so that the heat preservation plate does not need to be additionally provided with a fixing structure, which is beneficial to simplify the structure of the heating module 10.

[0098] At least one of the plurality of second heaters 300 is independently controlled, improving the flexibility of the control of the plurality of second heaters 300, so as to heat the peripheral reactors 120 in a targeted manner, thereby ensuring the uniformity of the heating of the plurality of reactors 120 by the heating module 10.

[0099] In the embodiments of the present application, the plurality of second heaters 300 are independently controlled, so as to separately control the temperature of the reactor 120 in the area where each second heater 300 is located, thereby improving the accuracy of the compensation heating of the second heater 300.

[0100] With reference to Figure 4 In some embodiments, the incubation seat 100 has a first middle line O1. Exemplarily, the extension direction of the first middle line O1 is parallel to the Figure 4 middle X-axis direction.

[0101] The plurality of reactors 120 are symmetrically arranged about the first middle line O1. It can be understood that the arrangement of the plurality of reactors 120 on both sides of the first middle line O1 is mirror-symmetrical.

[0102] The plurality of second heaters 300 are symmetrically arranged about the first middle line O1.

[0103] With reference to Figure 4 In some embodiments, the incubation seat 100 has a second middle line O2, and the first middle line O1 is perpendicular to the second middle line O2. Exemplarily, the extension direction of the second middle line O2 is parallel to the Figure 3 middle Y-axis direction.

[0104] The plurality of reactors 120 are symmetrically arranged about the second middle line O2, and it can be understood that the arrangement of the plurality of reactors 120 on both sides of the second middle line O2 is mirror-symmetrical. Thus, since the first middle line O1 and the second middle line O2 are perpendicular, the plurality of reactors 120 are arranged in a rectangular matrix on the bottom plate 110, which is convenient for experimental operation.

[0105] The plurality of second heaters 300 are symmetrically arranged about the second middle line O2.

[0106] Thus, the arrangement of the plurality of second heaters 300 on both sides of the first middle line O1 is mirror-symmetrical, and the arrangement of the plurality of second heaters 300 on both sides of the second middle line O2 is mirror-symmetrical, so that the plurality of second heaters 300 are symmetrically arranged on the incubation seat 100, which is beneficial to improve the uniformity of the sample temperature.

[0107] In some embodiments, with reference to Figure 5 , the incubation seat 100 is arranged with the plurality of reactors 120 in the direction of the first middle line O1, and the incubation seat 100 is arranged with the plurality of reactors 120 in the direction of the second middle line O2. In other words, the plurality of reactors 120 are arranged in a rectangular matrix on the bottom plate 110.

[0108] Among them, the four second heaters 300 are respectively used for heating the reactors 120 at the four top corners of the periphery.

[0109] From the center of the first heater 200 to the outside, the heat gradually decreases. The reactors 120 located at the four top corners are in the low temperature area, and the four second heaters 300 are used to compensate the temperature of the reactors 120 at the four top corners, which can improve the uniformity of the temperature distribution of the incubation seat 100, thereby improving the uniformity of the temperature among the multiple reactors 120.

[0110] In some embodiments, the first heater 200 is provided with one. In this way, the structure of the heating module 10 is simple, and the control logic is simple.

[0111] In other embodiments, the first heater 200 is provided with multiple, and the multiple first heaters 200 are arranged in a rectangular matrix on the side of the bottom plate 110 away from the reactors 120.

[0112] In this way, the size of each first heater 200 can be small, which is convenient for processing and installation. Moreover, the multiple first heaters 200 can reduce the temperature gradient, which is conducive to improving the uniformity of heating the incubation seat 100.

[0113] When the first heater 200 is provided with multiple, since the temperature of the first heater 200 decreases from the center to the edge, the temperature at the adjacent position of the adjacent two first heaters 200 is relatively low. The at least one second heater 300 arranged at the adjacent position of the adjacent two first heaters 200 can improve the uniformity of the temperature of the multiple reactors 120.

[0114] When the first heater 200 is in the form of a sheet, the temperature of the end surface of the heat sink 520 generally shows a trend of high in the middle and low at the four corners. Due to the heat dissipation risk of the heat sink 520, the thermal resistance of the heat transfer surface, etc., there is also a difference between the temperatures at the four corners, which further leads to the power output by the heat sink 520 on the plate surface of the incubation seat 100 also showing a trend of high in the middle and low at the four corners, i.e. the power in the middle of the heat sink 520 is high, and the power at the four corners is low.

[0115] Next, taking 4×8 reactors 120 as an example. In combination with Figure 5 , the first heater 200 is provided with two, and the two first heaters 200 are arranged side by side along the length direction of the incubation seat 100 (corresponding to the X-axis direction in Figure 6 ). Among them, the temperature field distribution on the bottom plate 110 shows an "8" shape, and the four top corners and the two middle waists are low temperature areas A; the center area of the two first heaters 200 is a high temperature area B.

[0116] In combination with Figure 4 , six temperature compensation areas C are formed on the bottom plate 110.

[0117] In combination with Figure 7The four second heaters 300a are used to compensate the temperature of the low-temperature areas A at the four corners, and the two second heaters 300b are used to compensate the temperature of the low-temperature areas A at the two waists.

[0118] The second heater 300a is configured to form a through hole 301, which is sleeved outside the reactor 120 at the corner to compensate the temperature of the low-temperature area at the corner.

[0119] The second heater 300b is configured to form two through holes 301, which can be respectively sleeved outside the two peripheral reactors 120 on both sides of the second middle line to compensate the temperature of the low-temperature area at the waist.

[0120] Due to the reasons such as assembly error, machining error and heat dissipation risk, there are differences between the six low-temperature areas, therefore, the six second heaters 300 are independently controlled to improve the uniformity of the temperature between the multiple reactors 120.

[0121] The heating module 10 of the embodiment of the application compensates the temperature of the low-temperature area by using the second heater 300 to improve the uniformity of the temperature distribution of the incubation seat 100, instead of compensating the temperature of the single reactor 120, so that the structure and control logic are simpler.

[0122] In combination with Figure 7 Taking 8x1 reactors 120 as an example, the eight reactors 120 are arranged at intervals along the first direction (corresponding to the X-axis direction in the figure). Figure 8 One first heater 200 can be used, and two second heaters 300a are used to compensate the temperature of the low-temperature areas at both ends of the first direction. The two second heaters 300a can be respectively configured to form a through hole 301 to be respectively sleeved outside the reactors 120 at both ends.

[0123] In combination with Figure 8 Taking 8x2 reactors 120 as an example, eight reactors 120 are arranged at intervals along the first direction (corresponding to the X-axis direction in the figure), and two reactors 120 are arranged at intervals along the second direction (corresponding to the Y-axis direction in the figure). Figure 8 Figure 9 One first heater 200 can be used, and two second heaters 300b are used to compensate the temperature of the low-temperature areas at both ends of the first direction. The two second heaters 300b can be respectively configured to form two through holes 301 to be respectively sleeved outside the two reactors 120 at both ends.

[0124] In combination with Figure 10 ​For example, for 8x4 reactors 120, one first heater 200 can be used, and four second heaters 300a can be used to compensate the temperature of the four low-temperature areas at the four corners, respectively. The four second heaters 300a can be respectively configured with one through hole 301 to be respectively sleeved outside the reactors 120 at the four corners.

[0125] For example, for 8x4 reactors 120, one first heater 200 can be used, and four second heaters 300a can be used to compensate the temperature of the four low-temperature areas at the four corners, respectively. The four second heaters 300a can be respectively configured with one through hole 301 to be respectively sleeved outside the reactors 120 at the four corners. Figure 11 For example, for 8x6 reactors 120, 2x2 first heaters 200 can be used, and four second heaters 300a can be used to compensate the temperature of the four low-temperature areas at the four corners, respectively. The four second heaters 300a can be respectively configured with one through hole 301 to be respectively sleeved outside the reactors 120 at the four corners. In addition, four second heaters 300b can be used to compensate the temperature of the low-temperature areas at the waists of the four sides, respectively. The four second heaters 300b can be respectively configured with two through holes 301 to be respectively sleeved outside the two reactors 120 at the waists.

[0126] For example, for 8x6 reactors 120, 2x2 first heaters 200 can be used, and four second heaters 300a can be used to compensate the temperature of the four low-temperature areas at the four corners, respectively. The four second heaters 300a can be respectively configured with one through hole 301 to be respectively sleeved outside the reactors 120 at the four corners. In addition, four second heaters 300b can be used to compensate the temperature of the low-temperature areas at the waists of the four sides, respectively. The four second heaters 300b can be respectively configured with two through holes 301 to be respectively sleeved outside the two reactors 120 at the waists. ​ For example, for 8x12 reactors 120, 3x2 first heaters 200 can be used, and four second heaters 300a can be used to compensate the temperature of the four low-temperature areas at the four corners, respectively. The four second heaters 300a can be respectively configured with one through hole 301 to be respectively sleeved outside the reactors 120 at the four corners. In addition, six second heaters 300b can be used to compensate the temperature of the low-temperature areas at the waists of the four sides, respectively. Two second heaters 300b are arranged in the long side direction, and one second heater 300b is arranged in the wide side direction. The six second heaters 300b can be respectively configured with two through holes 301 to be respectively sleeved outside the two reactors 120 at the waists.

[0127] The above is only illustrative, and is not a limitation on the number of reactors 120, the arrangement of the first heaters 200 and the second heaters 300.

[0128] In the above description, the description of the terms "one embodiment", "some embodiments", "an example", "a specific example", or "some examples" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, a person skilled in the art can combine and combine different embodiments or examples described in the specification and the features of different embodiments or examples without contradiction.

[0129] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, but not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. A detection device, characterized by The heating module comprises at least one heating module; the heating module comprises a control unit, an incubation seat, a first heater and a plurality of second heaters; The control unit is electrically connected with the first heater and the plurality of second heaters respectively; The incubation seat comprises a bottom plate and a plurality of reactors arranged on the bottom plate, and the reactors are used for placing sample containers; The first heater is located on the side of the bottom plate away from the reactors; The plurality of second heaters are arranged on the incubation seat and are used for heating a plurality of peripheral reactors respectively, at least one of the plurality of second heaters is independently controlled, and the heating power of the second heater is lower than that of the first heater.

2. The detection device of claim 1, wherein, At least one of the second heaters is arranged on the bottom plate.

3. The detection device of claim 2, wherein, At least one of the second heaters is provided with at least one through hole, so that the second heater is sleeved outside one of the peripheral reactors.

4. The detection device of claim 1, wherein, At least one of the second heaters is arranged on the side wall of the reactor.

5. The detection device according to any one of claims 1 to 4, characterized in that The incubation seat has a first center line, and the plurality of reactors are symmetrically arranged about the first center line; The plurality of second heaters are symmetrically arranged about the first center line.

6. The detection device of claim 5, wherein, The plurality of reactors are arranged in a rectangular matrix on the bottom plate; Four second heaters are arranged for heating the reactors at the four top corners respectively.

7. The detection device according to any one of claims 1 to 4, characterized in that The first heater is arranged in a plurality of rectangular matrix on the side of the bottom plate away from the reactors; At least one second heater is arranged at the adjacent position of adjacent two first heaters.

8. The detection device according to any one of claims 1 to 4, characterized in that The second heater is in the form of a sheet.

9. The detection device according to any one of claims 1 to 4, characterized in that The heating module further comprises a heat preservation structure arranged above the incubation seat; The heating module further comprises a heat sink in contact with the side of the first heater away from the incubation seat; a heat-conducting gasket is arranged between the heat sink and the first heater.

10. The detection device according to any one of claims 1 to 4, characterized in that A heat spreading sheet is arranged between the first heater and the bottom plate, the heat spreading sheet is configured to have a first heat conductivity coefficient on the surface of the bottom plate, and has a second heat conductivity coefficient in the direction perpendicular to the surface of the bottom plate, and the first heat conductivity coefficient is greater than the second heat conductivity coefficient.

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