Detection device

Abstract

The utility model relates to the technical field of temperature control, in particular to detection equipment. The utility model aims to solve the problem that the result accuracy is affected due to non-uniform temperature distribution on an existing metal module. The detection equipment provided by the utility model comprises at least one heating module, a fixing groove is formed in an incubation seat of the heating module, and a positioning groove is formed in the groove bottom wall of the fixing groove to accommodate a temperature sensor. The fixing piece is fixed in the fixing groove and arranged outside the temperature sensor in a sleeving mode so that the temperature sensor can be tightly pressed in the positioning groove. And the elastic piece is arranged between the fixing piece and the temperature sensor, and the temperature sensor is elastically pressed in the positioning groove, so that possible movement or looseness of the temperature sensor in the operation process is reduced, the installation reliability and stability of the temperature sensor are improved, and the mechanical stability and temperature measurement reliability of the temperature sensor are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of temperature control, in particular to a detection device. BACKGROUND

[0002] A gene amplification instrument is a basic instrument for polymerase chain reaction (PCR) in the biomedical field. It can provide a stable and adjustable temperature environment and is widely used as an instrument for DNA fragment amplification. The working principle of the gene amplification instrument is to complete the natural replication process similar to DNA denaturation-replication-extension through three basic reaction steps of denaturation-annealing-extension. The execution process of the three steps must accurately control the temperature of the sample, otherwise the result will be biased.

[0003] In the related art, the PCR heating module includes a metal module and a semiconductor refrigerator. The semiconductor refrigerator can heat and cool the metal module. A plurality of sample holes are provided on the metal module, and the sample holes can be used to place sample test tubes. A temperature sensor is also installed on the metal module to detect the temperature of the metal module.

[0004] However, the existing temperature sensor installation method has poor stability. With the extension of the use time of the instrument, the position of the temperature sensor is prone to shift, affecting the accuracy of temperature detection, and further affecting the accuracy and consistency of the sample detection result. CONTENT OF THE INVENTION

[0005] The present application provides a detection device to solve the technical problem of poor stability of the existing temperature sensor installation method affecting the accuracy of temperature detection.

[0006] To solve the above technical problems, the present application adopts the following technical solutions:

[0007] The present application provides a detection device, comprising: at least one heating module, the heating module comprising an incubation seat, a temperature sensor, a fixing member and an elastic member;

[0008] The incubation seat comprises a base, and a fixing groove and a plurality of reaction containers provided on the base. The reaction containers are used to place sample containers, and the groove bottom wall of the fixing groove is provided with a positioning groove.

[0009] The temperature sensor is installed in the positioning groove;

[0010] The fixing member is fixed in the fixing groove and is sleeved outside the temperature sensor;

[0011] The elastic member is elastically compressed between the temperature sensor and the fixing member.

[0012] Compared with the prior art, the detection device provided by the first aspect of the present application has the following advantages:

[0013] The detection device provided by the present application is provided with a fixing groove on the incubation seat of the heating module, and a positioning groove is arranged on the groove bottom wall of the fixing groove to accommodate the temperature sensor. The fixing member is fixed in the fixing groove and is sleeved outside the temperature sensor to press the temperature sensor tightly in the positioning groove. An elastic member is arranged between the fixing member and the temperature sensor to elastically press the temperature sensor in the positioning groove, which reduces the possible movement or loosening of the temperature sensor during operation, improves the reliability and stability of the installation of the temperature sensor, and improves the mechanical stability and temperature measurement reliability of the temperature sensor.

[0014] As an improvement of the above-mentioned detection device of the present application, the bottom end of the fixing member is provided with an accommodation groove, and the groove opening of the accommodation groove faces the groove bottom wall of the fixing groove. The elastic member is elastically pressed in the accommodation groove.

[0015] As an improvement of the above-mentioned detection device of the present application, the groove side wall of the fixing groove is provided with a wire passing opening, and one end of the temperature sensor provided with a connecting terminal faces the wire passing opening.

[0016] As an improvement of the above-mentioned detection device of the present application, the elastic member is an elastic heat-conducting member.

[0017] As an improvement of the above-mentioned detection device of the present application, the center of the groove bottom wall of the fixing groove coincides with the center of the groove bottom wall of the positioning groove.

[0018] As an improvement of the above-mentioned detection device of the present application, the base includes a mounting block and a bottom plate, the mounting block and the plurality of reaction containers are arranged on the bottom plate, and the mounting block is located in the interval of at least two reaction containers. The fixing groove is arranged in the mounting block.

[0019] As an improvement of the above-mentioned detection device of the present application, the top end of the mounting block is lower than the top end of the reaction container.

[0020] As an improvement of the above-mentioned detection device of the present application, the bottom plate, the plurality of reaction containers and the mounting block are integrally formed as an integral member.

[0021] As an improvement of the above-mentioned detection device of the present application, the heating module further comprises a heater in contact with the incubation seat, the heater being located on the side of the incubation seat away from the fixed groove; the incubation seat has a first lower surface facing the heater; the incubation seat is provided with a mounting flange protruding from the edge of the incubation seat; the mounting flange is provided with a first mounting hole; the heating module further comprises a heat sink and a fastener, the fastener being fixedly connected with the heat sink through the first mounting hole.

[0022] As an improvement of the above-mentioned detection device of the present application, the mounting flange has a second lower surface facing the heater, the second lower surface and the first lower surface have a first interval in a first direction, and the second lower surface is located on the side of the first lower surface away from the heater; wherein the first direction is perpendicular to the first lower surface.

[0023] As an improvement of the above-mentioned detection device of the present application, the first lower surface is in contact with the heater; the second lower surface has a second interval between one end facing the heater and the heater.

[0024] As an improvement of the above-mentioned detection device of the present application, the first interval is greater than 0.5mm.

[0025] As an improvement of the above-mentioned detection device of the present application, the second interval is greater than 1.0mm.

[0026] As an improvement of the above-mentioned detection device of the present application, the positioning groove is in surface contact with the temperature sensor.

[0027] As an improvement of the above-mentioned detection device of the present application, a heat-conducting medium is arranged between the temperature sensor and the groove wall of the positioning groove.

[0028] In addition to the above-mentioned technical problems solved by the present application, technical features constituting the technical solutions, and the beneficial effects brought by these technical features, other technical problems solved by the detection device provided by the present application, other technical features included in the technical solutions, and the beneficial effects brought by these technical features will be further described in detail in the specific embodiments. BRIEF DESCRIPTION OF DRAWINGS

[0029] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the description of the embodiments of the present application or the prior art. Obviously, the drawings in the following description are only some of the embodiments of the present application, and these drawings and the text description are not intended to limit the scope of the present application in any way, but to explain the present application to those skilled in the art by reference to the specific embodiments. Other drawings can also be obtained by those skilled in the art without creative effort.

[0030] Figure 1 Structure diagram of a heating module provided for some embodiments of the present application;

[0031] Figure 2 Exploded view of a heating module provided for some embodiments of the present application;

[0032] Figure 3 Structure diagram of an incubation seat and a heater provided for some embodiments of the present application;

[0033] Figure 4 Partial structure exploded view of a heating module provided for some embodiments of the present application;

[0034] Figure 5 Top view of an incubation seat provided for some embodiments of the present application;

[0035] Figure 6 Sectional exploded view of a partial structure of a heating module provided for some embodiments of the present application;

[0036] Figure 7 Sectional view of a partial structure of a heating module provided for some embodiments of the present application;

[0037] Figure 8 Sectional view of a partial structure of a heating module provided for some embodiments of the present application;

[0038] Figure 9 Deformation diagram of an incubation seat when fixed provided for some embodiments of the present application;

[0039] Figure 10 Deformation diagram of an incubation seat when fixed provided for some embodiments of the present application;

[0040] Figure 11 Sectional view of an incubation seat and a heater provided for some embodiments of the present application;

[0041] Figure 12 For Figure 11 Enlarged schematic view of the P area in the middle.

[0042] Explanation of reference signs:

[0043] 10: heating module;

[0044] 100: incubation seat; 101: base; 110: bottom plate; 120: reaction container; 130: mounting block; 131: fixing groove; 132: positioning groove; 133: wire passing opening; 140: first lower surface; 141: heat transfer area; 150: mounting flange; 151: second lower surface; 152: first mounting hole; 160: fastener; 170: side surface;

[0045] 200: temperature sensor;

[0046] 300: fixing member; 310: containing groove;

[0047] 400: elastic member;

[0048] 500: heater;

[0049] 600: heat preservation structure;

[0050] 710: heat spreading sheet; 720: heat sink; 721: second mounting hole; 730: heat conducting gasket. DETAILED DESCRIPTION

[0051] Embodiments of the present application are described in detail below with reference to examples illustrated in the accompanying drawings, in which the same or similar reference numerals represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by reference to the drawings are exemplary and are intended to explain the present application, and cannot be understood as limiting the present application.

[0052] In combination Figure 1 and Figure 2 , the embodiments of the present application provide a detection device which can perform amplification operation on a sample containing target DNA.

[0053] The detection device can include a housing, and at least one heating module 10 arranged in the housing, the heating module 10 can heat the sample in the sample container to complete the thermal cycle process of the sample. In some implementations, the detection device can include multiple heating modules 10 to increase the amount of samples processed simultaneously.

[0054] In some embodiments, the housing can further include a detection module and a scheduling mechanism, etc. The scheduling mechanism can transfer the sample container to the heating module 10 for thermal cycle operation, and after the thermal cycle operation is completed, the sample container is transferred to the detection module for detection to obtain the detection result.

[0055] Among them, the detection device can be a gene amplification instrument or an amplification analyzer, etc. The embodiments of the present application do not limit this.

[0056] As shown in Figure 1 and Figure 2 The heating module 10 includes an incubation seat 100, a control unit and a heater 500, wherein the sample container can be placed on the incubation seat 100, the control unit is used to control the heating temperature of the heater 500 to the incubation seat 100, so as to control the heating temperature of the sample in the sample container.

[0057] The incubation seat includes a base 101 and a plurality of reaction containers 120 arranged on the base 101. The reaction container 120 is configured to form a receiving groove 310 with a top port for accommodating the sample container. The sample container is used to accommodate the sample, and the sample container can be a sample tube, for example.

[0058] In some embodiments, the incubation seat 100 can be a metal seat, which can improve the heat transfer efficiency.

[0059] In some embodiments, referring to Figure 6 and Figure 7 The base 101 includes a bottom plate 110, and the reaction container 120 is arranged on the bottom plate 110.

[0060] The plurality of reaction containers 120 are arranged in a rectangular matrix on the bottom plate 110. For example, the sample container usually adopts an eight-tube. Therefore, the number of reaction containers 120 in a single row and / or a single column on the bottom plate 110 is a multiple of eight, which facilitates the placement of the sample container.

[0061] The heater 500 can be an electric heater 500, a semiconductor heater 500, etc. In some embodiments of the present application, the heater 500 is a semiconductor heater 500, which can quickly rise and fall in temperature, and the temperature control of the semiconductor heater 500 is more accurate, which is crucial for efficient PCR reaction.

[0062] In some embodiments, the heater 500 is a sheet-shaped heater, which can have a larger contact area to improve the uniformity of heating the plurality of reaction containers 120, and also facilitate reducing the overall volume of the heating module 10.

[0063] In some embodiments, the heater 500 is provided with one. Such a setting makes the structure of the heating module 10 simple and the control logic simple.

[0064] In other embodiments, the heater 500 is provided with a plurality, and the plurality of heaters 500 are arranged in a rectangular matrix on the side of the bottom plate 110 away from the reaction containers 120.

[0065] Such a setting can make the size of each heater 500 smaller, which is convenient for processing and installation. Moreover, the plurality of heaters 500 can reduce the temperature gradient, which is conducive to improving the uniformity of heating the incubation seat 100.

[0066] The heater 500 is located on the side of the base plate 110 opposite to the reaction vessel 120. Figure 2 In the direction shown, a reaction vessel 120 is provided on the top surface of the base plate 110, and a heater 500 is provided on the bottom surface of the base plate 110.

[0067] Continue to refer to Figure 2 In some embodiments, the heating module 10 includes a heat preservation structure 600, which is disposed above the incubation base 100 to improve the heat preservation performance of the incubation base 100.

[0068] The insulation structure 600 may include an insulation board, which may be insulating foam. The insulation board has clearance openings to allow passage of the sample container. Thus, at least a portion of the sample container can be exposed through the insulation board, facilitating experimental operations.

[0069] The insulation structure 600 may include an insulated face shield positioned above the insulation plate. The face shield has clearance holes to allow at least a portion of the sample container to be exposed through these holes for ease of operation.

[0070] In some embodiments, the heat insulation mask is fixed to the radiator 720 by screws to clamp the heat insulation plate between the heat insulation mask and the incubation base 100. The heat insulation plate does not require an additional fixing structure, which helps to simplify the structure of the heating module 10.

[0071] In some embodiments, a heat spreader 710 is provided between the heater 500 and the base plate 110. The heat spreader 710 is configured to have a first thermal conductivity on the surface of the base plate 110 and a second thermal conductivity in a direction perpendicular to the surface of the base plate 110. The first thermal conductivity is greater than the second thermal conductivity.

[0072] exist Figure 2 In the directions shown, the surface of the base plate 110 is parallel to the XY plane, and the direction perpendicular to the surface of the base plate 110 is... Figure 2 In the Z-axis direction, the thermal conductivity of the heat spreader 710 in the plane parallel to the XY plane is greater than its thermal conductivity in the Z-axis direction.

[0073] For example, the heat spreader 710 can be anisotropic materials such as graphene sheets or graphite sheets.

[0074] In this embodiment, by setting a heat spreader 710 as a thermal interface material, the temperature uniformity within the plate surface of the base plate 110 is improved, which is beneficial to improving the accuracy of the PCR reaction.

[0075] Continue to refer to Figure 2In the embodiments of the present application, the heater 500 is a semiconductor heater 500, which has a cold end and a hot end. The temperature of the cold end decreases while the hot end is heating. If one of the cold end and the hot end is not cooled in time, the heat of the other end will be affected.

[0076] To this end, in some embodiments, the heating module 10 further comprises a heat sink 720, which is in contact with the side of the heater 500 away from the incubation seat 100. The heat sink 720 is configured to dissipate the heat released by the heater 500 during operation.

[0077] In some embodiments, a heat-conducting gasket 730 is arranged between the heat sink 720 and the heater 500 to reduce the heat transfer resistance between the heater 500 and the first heat sink 720 and improve the heat dissipation efficiency of the heat sink 720.

[0078] Continuing to refer to Figure 2 and Figure 3 , the incubation seat 100 can be fixed to the heat sink 720, and the heater 500, the heat spreader 710, and the heat-conducting gasket 730 are clamped between the bottom plate 110 of the incubation seat 100 and the heat sink 720. The heater 500, the heat spreader 710, and the heat-conducting gasket 730 are arranged in a direction from top to bottom (corresponding to the positive direction to the negative direction of the Z axis in Figure 2 ).

[0079] In some embodiments, the bottom plate 110 of the incubation seat 100 and the heat sink 720 can be fixedly connected by fasteners, and the connection is stable and reliable.

[0080] For example, the bottom plate 110 and the heat sink 720 are fixedly connected by fasteners 160. The fasteners 160 can be plastic fasteners, which have a large thermal resistance and can reduce heat loss of the incubation seat 100, thereby facilitating improvement of the temperature uniformity of the incubation seat 100.

[0081] In combination Figure 2 to Figure 4 , in some embodiments, the incubation seat 100 is provided with a mounting flange 150. Specifically, the bottom plate 110 is provided with the mounting flange 150, and the mounting flange 150 is fixed to the heat sink 720 by the fasteners 160.

[0082] In some embodiments, in combination Figure 2 and Figure 3 , the mounting flange 150 is provided with a first mounting hole 152. The heat sink 720 is provided with a second mounting hole 721. The heat sink 720 and the mounting flange 150 are fixedly connected by the fasteners 160 penetrating the first mounting hole 152 and the second mounting hole 721. In this way, the incubation seat 100 and the heat sink 720 are fixedly connected stably and reliably.

[0083] Exemplarily, the fastener 160 can be a plastic screw, and the second mounting hole 721 can be a threaded hole. The plastic screw is screwed through the first mounting hole 152 and the second mounting hole 721 to achieve the fixed connection of the incubation seat 100 and the heat sink 720.

[0084] Exemplarily, the fastener 160 can include a bolt and a nut. The bolt is screwed through the first mounting hole 152 and the second mounting hole 721 and the nut to achieve the fixed connection of the incubation seat 100 and the heat sink 720.

[0085] In Figure 2 In the shown direction, the bottom plate 110 is arranged parallel to the XY plane, and the bottom plate 110 has a side surface perpendicular to the XY plane, and the mounting flange 150 is connected to the side surface, which is beneficial to improve the reliability of the contact between the bottom plate 110 and the heater 500 and ensure the temperature transfer efficiency.

[0086] Exemplarily, the bottom plate 110 has a long side surface parallel to the XZ plane, and the mounting flange 150 is connected to the long side surface. A plurality of mounting flanges 150 are arranged along the long side surface of the bottom plate 110 to improve the reliability of the fixed connection of the incubation seat 100 and the heat sink 720.

[0087] In some embodiments of the present application, in combination Figure 5 In some embodiments, the incubation seat 100 has a first median line O1 and a second median line O2, and the first median line O1 is perpendicular to the second median line O2. Exemplarily, the extension direction of the first median line O1 is parallel to the Figure 5 X-axis direction, and the extension direction of the second median line O2 is parallel to the Figure 5 Y-axis direction. The plurality of reaction containers 120 are symmetrically arranged about the first median line O1, and the plurality of reaction containers 120 are symmetrically arranged about the second median line O2. In other words, the plurality of reaction containers 120 are arranged in a rectangular matrix on the bottom plate 110.

[0088] In some embodiments, the edge of the bottom plate 110 is connected with a plurality of mounting flanges 150, the plurality of mounting flanges 150 are symmetrically arranged about the first median line O1, and the plurality of mounting flanges 150 are symmetrically arranged about the second median line O2.

[0089] In this way, the force on the edge of the incubation seat 100 is balanced, which is beneficial to ensure the reliability of the contact between the incubation seat 100 and the heater 500 and reduce the uneven heating caused by poor contact between the incubation seat 100 and the heater 500.

[0090] In Figure 2 to Figure 5In the shown structure, the mounting flange 150 is provided only on two opposite sides of the bottom plate 110, which is not restrictive. The mounting flange 150 can be provided on all four sides of the bottom plate 110. Among them, the side of the bottom plate 110 is perpendicular to the plate surface of the bottom plate 110, and the plate surface of the bottom plate 110 is parallel to the XY plane.

[0091] With reference to the foregoing Figure 4 and Figure 5 In some embodiments, the heating module 10 further comprises a temperature sensor 200 for detecting the heating temperature of the incubation seat 100.

[0092] In order to install the temperature sensor 200, the incubation seat 100 is provided with a fixing groove 131, wherein the fixing groove 131 provided on the base 101 is located on the side of the incubation seat 100 away from the heater 500. The fixing groove 131 is formed by a groove side wall and a groove bottom wall, and the groove opening of the fixing groove 131 is away from the base 101 of the incubation seat 100.

[0093] In some embodiments, the base 101 further comprises a mounting block 130, the mounting block 130 is arranged on the bottom plate 110, and the mounting block 130 is located within the interval of at least two reaction containers 120; the fixing groove 131 is arranged in the mounting block 130. In this way, the fixing groove 131 can be arranged on the mounting block 130, and the mounting block 130 can also be used to improve the structural strength of the incubation seat 100, so as to avoid directly opening a groove on the bottom plate 110 to affect the structural stability of the incubation seat 100.

[0094] In other embodiments, the fixing groove 131 can be directly arranged on the bottom plate 110 of the incubation seat 100, which is simple in structure.

[0095] In some embodiments, the bottom plate 110, the plurality of reaction containers 120 and the mounting block 130 are an integral part integrally formed. In this way, the structural stability of the incubation seat 100 can be ensured, and the thermal resistance between the mounting block 130 and the bottom plate 110 and the mounting block 130 and the reaction container 120 can be reduced, the heat conduction efficiency can be improved, and the accuracy of temperature detection of the temperature sensor 200 can be improved; the manufacturing process of the incubation seat 100 can be simplified, and the production cost can be reduced.

[0096] In some embodiments, in combination with Figure 6 The top end of the mounting block 130 is lower than the top end of the reaction container 120, which can avoid interference between the mounting block 130 and the heat preservation structure 600. In this way, the mounting block 130 has no effect on the appearance size of the incubation seat 100, so that no improvement is needed for other structures of the heating module 10, which is beneficial to simplify the heating module 10.

[0097] With reference to the foregoing Figure 5In some embodiments, the mounting block 130 is arranged at the intersection of the four reaction containers 120 and is in contact with the four reaction containers 120, and the four reaction containers 120 are arranged in a rectangular matrix. In this way, the space on the base plate 110 can be effectively utilized, and the compactness of the incubation seat 100 structure can be improved; the mounting block 130 is in contact with the four reaction containers 120, which can improve the uniformity of heat conduction and thus improve the uniformity of temperature detection; and the arrangement of the mounting block 130 can also improve the overall structural stability of the incubation seat 100.

[0098] In combination Figure 6 , the bottom wall of the fixing groove 131 is provided with a positioning groove 132, and the positioning groove 132 is formed by the side wall and the bottom wall. The direction of the groove opening of the positioning groove 132 is the same as that of the groove opening of the fixing groove 131. The temperature sensor 200 is installed in the positioning groove 132.

[0099] In some embodiments, the positioning groove 132 can be arranged on the base plate 110 and located in the fixing groove 131.

[0100] In some embodiments, as shown in Figure 5 , the positioning groove 132 can be a waist-shaped groove, which is simple to process. However, this is not a limitation on the shape of the positioning groove 132. For example, the positioning groove 132 can also be a rectangular groove, etc.

[0101] In some embodiments, a heat-conducting medium is arranged between the temperature sensor 200 and the groove wall of the positioning groove 132, which improves the stability of the contact between the temperature sensor 200 and the groove wall of the positioning groove 132, and is conducive to improving the uniformity of heat transfer between the groove wall of the positioning groove 132 and the temperature sensor 200, thereby facilitating the improvement of the accuracy of temperature detection of the temperature sensor 200. The groove wall of the positioning groove 132 includes the side wall and the bottom wall.

[0102] In some embodiments, the temperature sensor 200 is in the shape of a column, and the positioning groove 132 and the temperature sensor 200 are in line contact.

[0103] In other embodiments, the positioning groove 132 and the temperature sensor 200 are in surface contact, which increases the contact area between the positioning groove 132 and the temperature sensor 200, thereby facilitating the transfer of heat from the groove wall of the positioning groove 132 to the temperature sensor 200.

[0104] For example, the bottom wall of the positioning groove is an arc-shaped bottom wall, which matches the shape of at least part of the column-shaped temperature sensor 200.

[0105] The heating module 10 further comprises a fixing member 300 fixed in the fixing groove 131 and sleeved outside the temperature sensor 200. The fixing member 300 fixes the temperature sensor 200 in the positioning groove 132.

[0106] The fixing manner of the fixing member 300 in the fixing groove 131 includes clamping, threaded connection, bonding and the like. The fixing member 300 can also be connected with the fixing groove 131 in an interference fit.

[0107] The fixing member 300 can be a metal member. The temperature sensor 200 can be pressed by the gravity of the fixing member 300. The fixing member 300 made of metal has good heat conduction performance, so that the temperature sensor 200 can be in a relatively stable environment.

[0108] In some embodiments, as shown in Figure 7 The top end of the temperature sensor 200 protrudes from the groove bottom wall of the fixing groove 131. Thus, the fixing member 300 can apply pressure to the temperature sensor 200, thereby improving the reliability of the installation of the temperature sensor 200.

[0109] In some embodiments, in combination with Figure 5 The groove side wall of the fixing groove 131 is provided with a wire passing opening 133. One end of the temperature sensor 200 provided with a connecting terminal faces the wire passing opening 133. The wire passing opening 133 can avoid the connecting terminal of the temperature sensor 200 and provide space for the connection of the connecting terminal and the cable.

[0110] In combination with Figure 7 The heating module 10 further comprises an elastic member 400 elastically compressed between the temperature sensor 200 and the fixing member 300. The elastic member 400 can reduce the possible movement or loosening of the temperature sensor 200 during operation, improve the reliability and stability of the installation of the temperature sensor 200, and improve the mechanical stability and temperature measurement reliability of the temperature sensor 200.

[0111] In some embodiments, the elastic member 400 is an elastic heat-conducting member. The heat can be transmitted to the temperature sensor 200 through the fixing member and the elastic heat-conducting member, the uniformity of the temperature around the temperature sensor 200 is improved, and thus the accuracy of the temperature detected by the temperature sensor 200 is improved.

[0112] Therefore, the embodiment of the present application sets the fixing groove 131 on the incubation seat 100, sets the positioning groove 132 in the fixing groove 131 to accommodate the temperature sensor 200, sets the fixing member 300 in the fixing groove 131, and elastically presses the temperature sensor 200 in the positioning groove 132 by the elastic member 400. By simple structure and process, the high-reliability installation of the temperature sensor 200 is realized, so that the multiple temperature sensors 200 can have better temperature detection consistency and glue block response time, and the accuracy of temperature detection is improved.

[0113] For example, the elastic heat-conducting member can be an elastic heat-conducting rubber pad, which has elasticity and good heat-conducting performance.

[0114] Continuing to refer to Figure 6 and Figure 7 , the bottom end of the fixing member 300 is provided with the accommodating groove 310, and the slot opening of the accommodating groove 310 faces the groove bottom wall of the fixing groove 131. The elastic member 400 is elastically pressed in the accommodating groove 310.

[0115] In this way, the positioning and installation of the elastic member 400 are facilitated, and the convenience of the installation of the elastic member 400 is improved. The elastic member 400 is limited, the possibility of the movement of the elastic member 400 relative to the fixing member 300 and the temperature sensor 200 is reduced, the stability of the installation of the temperature sensor 200 is improved, and the possibility of the displacement of the temperature sensor 200 is reduced.

[0116] In combination Figure 5 , in some embodiments, the center of the groove bottom wall of the fixing groove 131 coincides with the center of the groove bottom wall of the positioning groove 132.

[0117] In this way, the temperature sensor 200 is installed at the center position of the fixing groove 131, so that the temperature sensor 200 is in a stable position, which is conducive to ensuring the accuracy and consistency of temperature measurement. The temperature sensor 200 is located at the center position of the fixing groove 131, can better contact the fixing member 300, and is conducive to improving the uniformity of heat conduction.

[0118] Referring to Figure 5 to Figure 8 , in some embodiments, the heating module 10 includes multiple temperature sensors 200 to improve the accuracy of temperature detection of the incubation seat 100. As Figure 5 shown, two temperature sensors 200 are arranged on the incubation seat 100.

[0119] The multiple temperature sensors 200 can be symmetrically arranged about the first center line O1, and / or the multiple temperature sensors 200 can be symmetrically arranged about the second center line O2. In this way, the uniformity of temperature detection of the incubation seat 100 can be improved.

[0120] In some possible implementation manners, the plurality of temperature sensors 200 can be arranged in a rectangular matrix on the incubation seat 100. The temperature of the incubation seat 100 is detected from a plurality of positions, which improves the accuracy of temperature detection of the incubation seat 100. Moreover, the number of temperature sensors 200 can be reduced, which is conducive to simplifying the heating module 10. The arrangement manner of the plurality of temperature sensors 200 is the same as the arrangement direction of the plurality of reaction containers 120, which is conducive to ensuring the symmetry of the structure of the incubation seat 100 and improving the mechanical stability of the incubation seat 100.

[0121] In some possible implementation manners, the temperature sensor 200 is located at the center position of the heater 500. Generally, the heat distribution is the most uniform at the center position of the heater 500, and the temperature sensor 200 is arranged at the center position of the heater 500, which is conducive to uniform temperature detection. Moreover, the temperature sensor 200 can be located at the center position of the heater 500, and the temperature change can be detected more quickly, so that rapid temperature feedback and detection are implemented.

[0122] The temperature control system of the heating module 10 includes feedback and adjustment. The temperature detected by the temperature sensor 200 is derived from the heating of the heater 500 on the incubation seat 100. If the thermal resistance distribution between the incubation seat 100 and the heater 500 is uneven, the response time of the temperature sensor 200 will be slowed down, and the temperature control system will be unstable.

[0123] Therefore, it is necessary to ensure that the contact planes of the incubation seat 100 and the heater 500 are not deformed, so as to ensure that the contact thermal resistances of the incubation seat 100 and the heater 500 are consistent.

[0124] With reference to Figure 2 In some embodiments, the bottom plate 110 of the incubation seat 100 is fixed to the heat sink 720 through the fastener 160. Specifically, the mounting flange 150 of the bottom plate 110 is provided with a mounting hole, the heat sink 720 is provided with a threaded hole, and the fastener 160 is threadedly connected through the mounting hole and the threaded hole, so as to fix the incubation seat 100 to the heat sink 720 and make the heater 500 clamped between the bottom plate 110 and the heat sink 720.

[0125] With reference to Figure 9 The fastener 160 presses the mounting flange 150 downward, so that a gap is generated between the bottom plate 110 and the heater 500, and the heat transfer surface is not in good contact. If the contact problem between the bottom plate 110 and the heater 500 is changed by increasing the structural strength of the incubation seat 100, the mass of the incubation seat 100 will be increased, which affects the heating and cooling rates.

[0126] Therefore, in combination with Figure 10The embodiment of the present application changes the structure of the mounting flange 150, forms a stepped structure on the mounting flange 150, reduces the stress transmission of the mounting flange 150 to the bottom plate 110, and reduces the deformation of the heat transfer surface on the bottom plate 110.

[0127] With reference to Figure 11 and Figure 12 The incubation seat 100 has a first lower surface 140 facing the heater 500, specifically, the surface of the bottom plate 110 facing the heater 500 forms the first lower surface 140. At least part of the first lower surface 140 is in contact with the heater 500, so that the heat of the heater 500 is transmitted towards the incubation seat 100.

[0128] In some embodiments of the present application, at least part of the first lower surface 140 is indirectly in contact with the heater 500 through the heat sink 710.

[0129] The mounting flange 150 has a second lower surface 151 facing the heater 500, and the second lower surface 151 has a first interval L1 with the first lower surface 140 along the first direction, and the second lower surface 151 is located on the side of the first lower surface 140 away from the heater 500.

[0130] In some embodiments, the second lower surface 151 can be arranged parallel to the first lower surface 140. This makes the structure of the whole incubation seat 100 and mounting flange 150 regular, which is conducive to ensuring the stability of the whole structure.

[0131] Wherein, the first direction is perpendicular to the first lower surface 140. In Figure 12 the direction shown, the first direction is parallel to the Z-axis direction.

[0132] In some embodiments, the first interval L1 is greater than 0.5 mm, so that a height difference can be formed between the first lower surface 140 and the second lower surface 151, and the transmission of stress is reduced.

[0133] With respect to the upper surface of the heater 500, in the first direction, the second lower surface 151 is higher than the first lower surface 140, so that the second lower surface 151 and the first lower surface 140 have a first interval L1 along the first direction. Wherein, the upper surface of the heater 500 is the surface of the heater 500 facing the incubation seat 100.

[0134] The embodiment of the present application reduces the transmission of stress to the incubation seat 100 by arranging the second lower surface 151 of the mounting flange 150 and the first lower surface 140 of the incubation seat 100 to have an interval, thereby reducing the deformation of the incubation seat 100, so that good contact between the incubation seat 100 and the heater 500 can be ensured.

[0135] With reference to Figure 11 and Figure 12In some embodiments, the first lower surface 140 comprises a heat transfer region 141 which is in contact with the heater 500. In other words, the region of the first surface which is in contact with the heater 500 is the heat transfer region 141.

[0136] It can be appreciated that the heat transfer region 141 can be in direct contact with the heater 500, reducing the thermal resistance between the heat transfer region 141 and the heater 500.

[0137] Alternatively, the heat transfer region 141 can be in indirect contact with the heater 500, for example, a graphene sheet or other heat spreading sheet 710 can be provided between the heat transfer region 141 and the heater 500 to improve the uniformity of heat transfer within the heat transfer region 141.

[0138] The second lower surface 151 has a second spacing L2 from the heater 500 at an end facing the heater 500.

[0139] In combination Figure 5 and Figure 12 , the mounting flange 150 protrudes from the edge of the base plate 110 in the Y-axis direction. In the Y-axis direction, the second lower surface 151 and the first lower surface 140 are at different heights, forming a side surface 170 therebetween, which can be perpendicular to the first lower surface 140. In this way, the second spacing L2 is the distance between the side surface 170 and the heater 500.

[0140] In this way, the heat transfer region 141 does not extend to the edge of the first lower surface 140, so that the second lower surface 151 and the heat transfer region 141 have a second spacing L2 therebetween, further reducing the stress of the fastener 160 on the mounting flange 150 being transmitted to the heat transfer region 141, thereby reducing the deformation of the heat transfer region 141.

[0141] In some embodiments, the second spacing is greater than 1.0 mm, which can avoid the second spacing being too small, causing the heat transfer region 141 to be affected by the stress of the fastener 160, thereby affecting the contact between the edge of the heat transfer region 141 and the incubation seat 100.

[0142] In the above description, the description with reference to the terms "one embodiment", "some embodiments", "an example", "a specific example", or "some examples" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, those skilled in the art can combine and combine the different embodiments or examples described in the specification and the features of the different embodiments or examples without contradiction, and the combination.

[0143] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solution deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. A detection device, characterized by The heating module comprises at least one incubation seat, a temperature sensor, a fixing member and an elastic member. The incubation seat comprises a base, a fixing groove and a plurality of reaction containers arranged on the base, the reaction containers being used for placing sample containers, and a positioning groove is arranged on the groove bottom wall of the fixing groove. The temperature sensor is installed in the positioning groove. The fixing member is fixed in the fixing groove and sleeved outside the temperature sensor. The elastic member is elastically compressed between the temperature sensor and the fixing member.

2. The detection device of claim 1, wherein, The bottom end of the fixing member is provided with a containing groove, and the groove opening of the containing groove faces the groove bottom wall of the fixing groove. The elastic member is elastically compressed in the containing groove.

3. The detection device of claim 1, wherein, The center of the groove bottom wall of the fixing groove coincides with the center of the groove bottom wall of the positioning groove.

4. The detection device according to any one of claims 1 to 3, characterized in that The base comprises a mounting block and a bottom plate, the mounting block and the plurality of reaction containers are arranged on the bottom plate, and the mounting block is located in the interval of at least two reaction containers; and the fixing groove is arranged in the mounting block.

5. The detection device of claim 4, wherein, The top end of the mounting block is lower than the top end of the reaction container; and / or, The bottom plate, the plurality of reaction containers and the mounting block are integrally formed as an integral piece.

6. The detection device according to any one of claims 1 to 3, characterized in that The heating module further comprises a heater in contact with the incubation seat, and the heater is located on the side of the incubation seat away from the fixing groove. The incubation seat has a first lower surface facing the heater; and the incubation seat is provided with a mounting flange protruding from the edge of the incubation seat. A first mounting hole is arranged on the mounting flange, and the heating module further comprises a heat sink and a fastener fixedly connected with the heat sink through the first mounting hole.

7. The detection device of claim 6, wherein, The mounting flange has a second lower surface facing the heater, and the second lower surface has a first interval with the first lower surface in a first direction, and the second lower surface is located on the side of the first lower surface away from the heater; wherein the first direction is perpendicular to the first lower surface.

8. The detection device of claim 7, wherein, The first lower surface is in contact with the heater. The second lower surface has a second interval with the heater between one end of the second lower surface facing the heater.

9. The detection device of claim 8, wherein, The first interval is greater than 0.5mm; and / or, The second interval is greater than 1.0mm.

10. The detection device according to any one of claims 1 to 3, characterized in that The positioning groove and the temperature sensor are in surface contact; and / or, A heat-conducting medium is arranged between the temperature sensor and the groove wall of the positioning groove.

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