Biological enzymolysis extraction reactor

Abstract

The utility model relates to a reactor, and provides the biological enzymolysis extraction reactor which comprises a base, a bracket I, a bracket II, a control cabinet, a reaction tank and the like, a second support is arranged on the right portion of the base, a control cabinet is fixedly arranged on the upper portion of the second support, a control panel is arranged on the control cabinet, two first supports are arranged on the top of the base, reaction tanks are vertically installed on the first supports, top covers are installed on the tops of the reaction tanks, the reaction tanks are sealed through the top covers, and reaction cavities are formed between the top covers and the interiors of the reaction tanks. According to the utility model, the double reaction tanks are connected in series, pre-reaction and further reaction are carried out on liquid, new reaction liquid can be immediately injected for pre-reaction after the pre-reaction liquid is pumped out in the pre-reaction tank body, and meanwhile, an automatic control system is arranged in a matched manner, so that efficient continuous operation and a stable enzymolysis reaction process are realized; when a certain amount of materials are treated, the overall processing time can be shortened, and the efficiency is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to a reactor especially to a biological enzymolysis extraction reactor. BACKGROUND

[0002] The biological enzymolysis extraction reactor is a kind of equipment using specific enzyme catalytic reaction, from plant, animal or microorganism etc. High-efficiency extraction of effective component of biomass, with the development of biotechnology and pharmaceutical industry, biological enzymolysis extraction technology gradually becomes the important means of extracting natural product because of its high selectivity, mild condition, environmental friendly etc. Advantage, traditional extraction method such as solvent extraction and chemical degradation has high energy consumption, serious pollution, effective component is easily destroyed etc. Problem, and biological enzymolysis extraction reactor realizes high-efficiency extraction under lower temperature and pH condition by simulating the enzymatic reaction process in vivo, improves extraction efficiency and product quality.

[0003] Although biological enzymolysis extraction technology has many advantages, but the prior art still has some deficiencies, first, the traditional biological enzymolysis extraction reactor is improved in enzyme and substrate contact efficiency, leads to long reaction time, influence production efficiency, second, the parameter control in the reaction process of existing equipment is not accurate enough, is dependent on artificial experience and carries out the operation of relevant steps, is difficult to monitor and adjust reaction condition in real time, leads to unstable product yield and quality, limits its application. UTILITY MODEL CONTENT

[0004] In order to overcome the shortcoming that the traditional biological enzymolysis extraction reactor is improved in enzyme and substrate contact efficiency, leads to long reaction time, influence production efficiency, second, the parameter control in the reaction process of existing equipment is not accurate enough, is dependent on artificial experience and carries out the operation of relevant steps, is difficult to monitor and adjust reaction condition in real time, leads to unstable product yield and quality, limits its application, the utility model aims at providing a biological enzymolysis extraction reactor that is provided with online monitoring system and can continuously operate when a certain amount of material is subjected to enzyme reaction, and overall shorten processing time.

[0005] The utility model discloses a technical scheme is: a kind of biological enzymatic extraction reactor, including base, support one, support two, control cabinet, reaction tank, lower discharge pipe, lower discharge pump, connecting pipe, liquid pump, end cover and temperature insulation cylinder, base right part is equipped with support two, support two upper portion is fixedly provided with control cabinet, control cabinet is equipped with control panel, the top of base is equipped with two support one, reaction tank is vertically installed on support one, the top of reaction tank is equipped with top cover, reaction tank is closed by top cover, top cover and reaction tank inside form a reaction cavity between, reaction tank outside is equipped with temperature insulation cylinder, there is a annular temperature adjustment interval between temperature insulation cylinder inner wall and reaction tank outer wall, medium pipeline is equipped on temperature insulation cylinder, medium pipeline is communicated with temperature adjustment interval, the side of reaction tank upper portion is equipped with liquid inlet pipe, and reaction tank lower portion is vertically equipped with lower discharge pipe, lower discharge pipe is communicated with reaction tank internal space, lower discharge pump is equipped on the pipeline of lower discharge pipe, connecting pipe is equipped between the lower discharge pipe of left side reaction tank and the liquid inlet pipe of right side reaction tank, and liquid pump is equipped on the pipeline of connecting pipe, and liquid pump is extracted into right side reaction tank in left side reaction tank.

[0006] Optionally, further comprising mounting rack and porous carrier, reaction tank is equipped with mounting rack in lower part, and porous carrier is embedded in mounting rack, and porous carrier is concentrically arranged with reaction tank, and the enzyme required during reaction is fixed on porous carrier.

[0007] Optionally, further comprising integrated detection module, and integrated detection module is arranged on the pipeline of connecting pipe, and the detection end of integrated detection module is inserted into connecting pipe.

[0008] Optionally, further comprising ultrasonic module and oscillation column, and ultrasonic module is arranged on top cover, and oscillation column is vertically arranged on the lower part of ultrasonic module, and oscillation column penetrates top cover and is inserted into reaction tank internal space, and oscillation column penetrates porous carrier and extends into reaction tank lower space, wherein, through hole is formed on porous carrier, and oscillation column and porous carrier do not contact each other.

[0009] Optionally, further comprising filter and liquid outlet pipe, and filter is connected to the lower end of lower discharge pipe of right side reaction tank, and liquid outlet pipe is arranged on one side of the lower part of filter.

[0010] Optionally, further comprising washing inlet pipe, shunt pipe and liquid discharge pump, and washing inlet pipe is arranged on top cover, and shunt pipe is connected to right side lower discharge pipe, and liquid discharge pump is arranged on the pipeline of shunt pipe.

[0011] Beneficial effect: 1, the utility model discloses a double reaction tank series connection design, and the liquid is pre-reacted and further reacted, after pre-reaction liquid is extracted in the tank body, can immediately inject new reaction liquid and pre-react, and simultaneously cooperate with the automatic control system arranged, realizes the continuous operation of high efficiency and stable enzymatic hydrolysis reaction process, when processing certain amount of material, can reduce the reaction time of overall processing, improves efficiency.

[0012] 2、The utility model discloses a method for immobilizing enzyme on porous carriers such as activated carbon, ceramic particles or cellulose materials, which have large specific surface area and can provide more enzyme attachment points, thus increasing the effective contact area of enzyme and substrate, and providing a good mass transfer environment, so that substrate and product can quickly diffuse in and out of the reaction area, improving the reaction rate and conversion rate. In addition, the immobilized enzyme can maintain stable performance in multiple reaction cycles, reducing enzyme loss and replenishment frequency.

[0013] 3、The utility model discloses a method for removing large particle impurities and suspended solids in the mixed solvent by connecting the solvent into the filter when the enzymatic reaction is completed, significantly reducing the processing pressure of the subsequent filtration centrifugation process, and reducing energy consumption and equipment wear.

[0014] 4、The utility model discloses a method for breaking the hydrogen bonds and other weak interactions between substrate molecules in the reaction tank by using the ultrasonic oscillation column to form microbubbles in the liquid and release high-energy shock waves and local high-temperature and high-pressure environment, promoting the contact between substrate and enzyme, and further accelerating the reaction process. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model.

[0016] Figure 2 It is a schematic diagram of the three-dimensional structure of the reaction tank, temperature insulation cylinder and connecting pipe and other components of the utility model.

[0017] Figure 3 It is a plane sectional view of part of the components of the utility model.

[0018] Figure 4 It is a schematic diagram of the three-dimensional structure of the mounting bracket and porous carrier of the utility model

[0019] Figure 5 It is a schematic diagram of the three-dimensional structure of the filter and liquid outlet pipe of the utility model.

[0020] Figure label name: 1-base, 2-bracket one, 21-bracket two, 22-control cabinet, 3-reaction tank, 31-lower discharge pipe, 32-lower discharge pump, 33-connecting pipe, 34-liquid pumping pump, 4-top cover, 41-temperature insulation cylinder, 410-temperature adjustment interval, 5-integrated detection module, 6-mounting bracket, 61-porous carrier, 7-ultrasonic module, 71-oscillation column, 8-washing inlet pipe, 81-shunt pipe, 82-liquid discharge pump, 9-filter, 91-liquid outlet pipe. DETAILED DESCRIPTION

[0021] The technical solutions in the embodiments of the utility model will be clearly and completely described below, obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the person skilled in the art without creative labor belong to the protection scope of the utility model.

[0022] Embodiment 1

[0023] A kind of biological enzymatic extraction reactor, as shown in Figures 1-4 It includes base 1, support one 2, support two 21, control cabinet 22, reaction tank 3, lower exhaust pipe 31, lower exhaust pump 32, connecting pipe 33, liquid pump 34, top cover 4 and temperature insulation cylinder 41, the right part of base 1 is equipped with support two 21, support two 21 upper portion is fixedly installed with control cabinet 22, control cabinet 22 is equipped with control panel, for operating and monitoring the whole reaction process.

[0024] Base 1 top is equipped with two support one 2, and each support one 2 is vertically installed with one reaction tank 3, and the top of reaction tank 3 is installed with detachable top cover 4, reaction tank 3 is closed by top cover 4, and a sealed reaction cavity is formed between top cover 4 and the inside of reaction tank 3, and top cover 4 and reaction tank 3 are equipped with detection instrument, these detection instruments are signal connected with control cabinet 22, and the temperature, pH value and other key parameters in reaction cavity are monitored in real time, and data is transmitted to control cabinet 22, realizes automatic monitoring and regulation, ensures that reaction condition is always in the best state, to improve reaction efficiency and product quality.

[0025] Two reaction tank 3 outer sides are each equipped with temperature insulation cylinder 41, and there is an annular temperature adjustment interval 410 between the inner wall of temperature insulation cylinder 41 and the outer wall of reaction tank 3, and medium pipeline is equipped on temperature insulation cylinder 41, which is communicated with temperature adjustment interval 410, by circulating cooling or heating medium (such as steam, water or heat conducting oil) in temperature adjustment interval 410, the temperature environment of reaction tank 3 can be accurately controlled, temperature insulation cylinder 41 is made of heat preservation material, effectively reduces heat loss, and reaction tank 3 is made of material with good heat conduction (such as stainless steel or glass), to ensure that the internal reaction material can quickly reach the set temperature, further improve the reaction efficiency.

[0026] The upper side of the reaction tank 3 is provided with a liquid inlet pipe for injecting reaction liquid, and the lower part of the reaction tank 3 is vertically provided with a lower discharge pipe 31, which is in communication with the internal space of the reaction tank 3. The lower discharge pipe 31 is provided with a lower discharge pump 32 on the pipeline, which is used to discharge the reacted liquid. The lower discharge pipe 31 of the left reaction tank 3 is connected with the liquid inlet pipe of the right reaction tank 3, and the pipeline of the connecting pipe 33 is provided with a liquid pumping pump 34. Through the liquid pumping pump 34, the pre-reaction liquid in the left reaction tank 3 can be pumped into the right reaction tank 3 for further reaction. This design enables the left reaction tank 3 to inject new reaction liquid immediately after completing the pre-reaction, and continue the pre-reaction, thereby realizing continuous operation

[0027] The specific working principle of the reactor is as follows:

[0028] Pre-reaction stage: The reaction liquid is first injected into the left reaction tank 3 through the liquid inlet pipe. The detector on the top cover 4 monitors the temperature, pH value and other key parameters in the reaction cavity in real time, and transmits the data to the control cabinet 22 to ensure that the reaction conditions are always in the best state. The temperature control interval 410 in the temperature insulation cylinder 41 circulates cooling or heating medium (such as steam, water or heat conducting oil) through the medium pipeline to accurately control the temperature environment of the reaction tank 3, so that the enzyme can play a role under the most suitable conditions.

[0029] Liquid transfer and further reaction: After the pre-reaction is completed, the liquid pumping pump 34 is started to pump the pre-reaction liquid in the left reaction tank 3 into the right reaction tank 3 through the connecting pipe 33 for further enzymatic reaction. In this process, the right reaction tank 3 is also equipped with a detector and a temperature insulation cylinder 41 to ensure accurate control of the reaction conditions to achieve efficient and stable enzymatic reaction.

[0030] Continuous operation: When the pre-reaction liquid in the left reaction tank 3 is pumped out, new reaction liquid can be injected immediately to continue the pre-reaction. This design enables the entire system to realize continuous operation, greatly improving production efficiency. At the same time, the lower discharge pump 32 is responsible for discharging the final reaction product from the lower discharge pipe 31 of the right reaction tank 3 to ensure that the reacted liquid is timely treated.

[0031] Centralized monitoring and control: The entire reaction process is centrally monitored and controlled by the control cabinet 22. The control panel is used to set and adjust the reaction parameters to ensure that each step is accurately performed according to the predetermined procedure. Real-time monitoring and feedback control system ensures the stability and consistency of the reaction conditions, reduces manual intervention, and thus improves the efficiency of enzymatic extraction and product quality

[0032] Example 2

[0033] Based on Example 1, such as Figure 3 and Figure 4As shown, the bioenzymatic extraction reactor further comprises a mounting frame 6 and a porous carrier 61, and the lower part of the reaction tank 3 is provided with the mounting frame 6, and the mounting frame 6 is embedded with the porous carrier 61, and the porous carrier 61 is concentrically arranged with the reaction tank 3, and the porous carrier 61 is used to fix the enzyme required in the reaction, and the specific material can be selected from activated carbon, ceramic particles or cellulose material and other substances with large specific surface area. These carriers can provide more enzyme attachment points.

[0034] In order to enhance the enzyme fixation effect of the porous carrier 61, the carrier material is first pretreated to increase the surface active site, for example, the hydrophilicity and surface roughness of the carrier are improved by acid-base treatment or surface modification technology, so as to increase the contact area and binding force of the enzyme molecules and the surface of the carrier. Then, by using the physical adsorption method, the enzyme solution is mixed with the pretreated carrier, and under suitable temperature (such as 25℃) and pH conditions, the enzyme molecules are adsorbed to the surface of the carrier through weak interactions such as hydrogen bonds and van der Waals forces, so as to ensure that the enzyme maintains high activity and stability during the reaction process. In addition, chemical cross-linking method can also be used to connect the enzyme and the functional groups on the surface of the carrier through covalent bond, further enhancing the efficiency of enzyme immobilization. The specific method is to use a cross-linking agent (such as glutaraldehyde) to cross-link the amino or carboxyl groups on the enzyme molecules with the functional groups on the surface of the carrier, forming a stable enzyme-carrier complex. For some enzymes, embedding method can also be used to wrap the enzyme in the microporous structure of the carrier, forming a stable complex to prevent the loss of enzyme molecules while maintaining its catalytic activity.

[0035] Through a series of related operations, the enzyme can be firmly fixed on the porous carrier 61, and due to the large specific surface area of the carrier, the efficiency and stability of the enzyme immobilization can be significantly improved. The presence of the porous carrier 61 not only increases the effective attachment points of the enzyme, but also provides a good mass transfer environment, so that the substrate and product can quickly diffuse into and out of the reaction area, improving the reaction rate and conversion rate. In addition, the fixed enzyme can maintain stable performance in multiple reaction cycles, reducing the loss and frequency of enzyme replenishment, and reducing production costs.

[0036] As shown in FIG. 1, the bioenzymatic extraction reactor comprises a reaction tank 3, an enzyme solution tank 4, a pump 5, a mounting frame 6 and a porous carrier 61. Figure 1 and Figure 2As shown, it also includes an integrated detection module 5. The integrated detection module 5 is installed on the connecting pipe 33, and the detection end of the integrated detection module 5 extends into the connecting pipe 33. The integrated detection module 5 is equipped with a variety of high-precision sensors, which can perform real-time detection of the solvent after the pre-reaction. The monitored parameters include temperature, pH value, enzyme concentration, and reaction product concentration. These data are fed back to the control cabinet 22 through the built-in communication interface, so that when the solvent is injected into the right reaction tank 3, the right reaction tank 3 can adjust the reaction conditions in time according to the solvent state, ensuring that the reaction process is always in the optimal state. This not only improves the controllability and accuracy of the reaction, but also enables early detection and correction of any abnormalities, further improving product quality and production efficiency.

[0037] Among them, such as Figures 1-3 As shown, an ultrasonic module 7 is provided on the top cover 4. A vertical oscillating column 71 is provided at the bottom of the ultrasonic module 7. The oscillating column 71 passes downward through the top cover 4 and into the internal space of the reaction vessel 3. The lower part of the oscillating column 71 passes through the porous carrier 61 and extends into the lower space inside the reaction vessel 3. The porous carrier 61 has through holes to ensure that the oscillating column 71 and the porous carrier 61 do not come into contact with each other. The ultrasonic module 7 generates high-frequency vibrations, which are transmitted to the liquid medium in the reaction vessel 3 through the oscillating column 71, generating a cavitation effect. This effect can form tiny bubbles in the liquid and burst them instantly, releasing high-energy shock waves and a local high-temperature and high-pressure environment. This helps to break the hydrogen bonds and other weak interactions between substrate molecules, promotes the contact between the substrate and the enzyme, and accelerates the reaction process. At the same time, ultrasonic vibration can also enhance the turbulence of the liquid, improve the mass transfer effect, and make the substrate and product more evenly distributed in the reaction system, further improving the reaction efficiency and conversion rate. The design of the oscillating column 71 ensures that the ultrasonic energy can be effectively transmitted to the bottom of the reaction vessel 3, especially around the porous carrier 61, without directly contacting the porous carrier 61, thus avoiding damage to the immobilized enzyme structure and ensuring the long-term stability and activity of the enzyme.

[0038] In addition, such as Figure 1 and Figure 5 As shown, it also includes a filter element 9 and a liquid outlet pipe 91. The lower end of the drain pipe 31 of the reaction vessel 3 on the right is connected to the filter element 9. The liquid outlet pipe 91 is provided on one side of the lower part of the filter element 9. Specifically, after the reaction is completed, the mixed solvent enters the filter element 9 through the drain pipe 31. The filter element 9 can adopt a multi-stage filtration structure, including a coarse filter layer and a fine filter layer, to effectively remove large particulate impurities and suspended solids in the mixed solvent. The coarse filter layer is mainly composed of filter screens or filter elements with larger pore sizes, which are used to initially intercept larger solid particles and incompletely dissolved substrates. The fine filter layer is composed of a finer filter membrane, which can further separate small particles and colloidal substances. The solvent after primary filtration is discharged from the liquid outlet pipe 91, which significantly reduces the processing pressure of subsequent filtration and centrifugation processes, and reduces energy consumption and equipment wear.

[0039] In a preferred embodiment, as shown in Figures 1-3 The washing inlet pipe 8 is arranged on the top cover 4 to guide the washing liquid into the inside of the reaction tank 3. The shunt pipe 81 is connected to the lower discharge pipe 31 on the right side. The liquid discharge pump 82 is arranged on the shunt pipe 81. During the washing process, the lower discharge pump 32 of the reaction tank 3 on the right side is closed, and the liquid discharge pump 82 on the shunt pipe 81 is opened, so that the washing liquid of the reaction tank 3 on the right side can be discharged through the shunt pipe 81. At the same time, the liquid discharge pump 82 of the reaction tank 3 on the left side is opened to ensure that the washing liquid can be smoothly discharged out of the equipment. The liquid pumping pump 34 on the connecting pipe 33 is closed to prevent the washing liquid from flowing into other pipeline systems, ensure the effectiveness and thoroughness of the washing process, and avoid the mixing of the washing liquid and other untreated materials, thereby improving the cleanliness and hygiene standard of the system. The design position of the washing inlet pipe 8 ensures that the washing liquid can uniformly cover every corner in the reaction tank 3, ensuring that there is no dead angle cleaning. In addition, the cooperation of the liquid discharge pump 82 and the shunt pipe 81 enables the washing liquid to be quickly discharged under high pressure, reducing the washing time and water consumption, and further improving the operation efficiency and environmental performance of the equipment.

[0040] By introducing the washing inlet pipe 8, the shunt pipe 81 and the liquid discharge pump 82, and combining the operation logic of closing the lower discharge pump 32 of the reaction tank 3 on the right side and opening the liquid discharge pump 82 of the reaction tank 3 on the left side, the efficient washing function is realized, and the system has higher automation level and better quality control ability, which significantly improves the performance and economic benefits of the entire system.

[0041] Although the present disclosure has been shown and described with respect to certain exemplary embodiments thereof, it should be understood by those skilled in the art that various changes in form and detail can be made therein without departing from the spirit and scope of the disclosure as defined by the appended claims and their equivalents. Therefore, the scope of the present disclosure should not be limited to the above-described embodiments, but should be determined only by the appended claims, and should be defined by the equivalents of the appended claims.

Claims

1. A biological enzymatic extraction reactor, comprising a base (1); characterized in that It also includes support one (2), support two (21), control cabinet (22), reaction tank (3), lower exhaust pipe (31), lower exhaust pump (32), connecting pipe (33), liquid pump (34), end cover and temperature insulation cylinder (41), the right part of base (1) is equipped with support two (21), the upper part of support two (21) is fixedly provided with control cabinet (22), control cabinet (22) is provided with control panel, the top of base (1) is equipped with two support one (2), the vertical installation of reaction tank (3) is carried out on support one (2), the top of reaction tank (3) is installed with top cover (4), reaction tank (3) is closed through top cover (4), a reaction cavity is formed between top cover (4) and the inside of reaction tank (3), the outside of reaction tank (3) is equipped with temperature insulation cylinder (41), there is a ring-shaped temperature adjustment interval (410) between the inner wall of temperature insulation cylinder (41) and the outer wall of reaction tank (3), medium pipeline is equipped on temperature insulation cylinder (41), medium pipeline is communicated with temperature adjustment interval (410), the upper part of reaction tank (3) is equipped with liquid inlet pipe on one side, and the lower part of reaction tank (3) is vertically provided with lower exhaust pipe (31), lower exhaust pipe (31) is communicated with the inside space of reaction tank (3), lower exhaust pump (32) is arranged on the pipeline of lower exhaust pipe (31), the lower exhaust pipe (31) of left reaction tank (3) and the liquid inlet pipe of right reaction tank (3) are equipped with connecting pipe (33), liquid pump (34) is arranged on the pipeline of connecting pipe (33), liquid pump (34) draws the liquid in left reaction tank (3) into right reaction tank (3).

2. The biocatalytic extraction reactor according to claim 1, characterized in that It also includes mounting bracket (6) and porous carrier (61), the lower part of reaction tank (3) is equipped with mounting bracket (6), the porous carrier (61) is embedded in mounting bracket (6), the porous carrier (61) is concentrically arranged with reaction tank (3), and the required enzyme in the reaction is fixed on the porous carrier (61).

3. The biocatalytic extraction reactor according to claim 2, characterized in that: It also includes integrated detection module (5), the pipeline of connecting pipe (33) is equipped with integrated detection module (5), and the detection end of integrated detection module (5) penetrates into connecting pipe (33).

4. The biocatalytic extraction reactor according to claim 3, characterized in that: It also includes ultrasonic module (7) and oscillation column (71), ultrasonic module (7) is arranged on top cover (4), ultrasonic module (7) is vertically provided with oscillation column (71) on the lower part, oscillation column (71) penetrates into the inside space of reaction tank (3) downward through top cover (4), and the lower part of oscillation column (71) penetrates through porous carrier (61) and extends into the lower space of reaction tank (3), wherein, the porous carrier (61) is provided with a through hole, and the oscillation column (71) and the porous carrier (61) do not contact each other.

5. The biogenic enzymatic extraction reactor according to claim 4, characterized in that It also includes filter (9) and liquid outlet pipe (91), the lower end of lower exhaust pipe (31) of right reaction tank (3) is connected with filter (9), one side of the lower part of filter (9) is equipped with liquid outlet pipe (91).

6. The biogenic enzymatic extraction reactor according to claim 5, characterized in that It also includes washing inlet pipe (8), shunt pipe (81) and liquid discharge pump (82), washing inlet pipe (8) is arranged on top cover (4), shunt pipe (81) is connected on the right lower exhaust pipe (31), and liquid discharge pump (82) is arranged on the pipeline of shunt pipe (81).

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