Fluorescein column chromatography separation device

Abstract

The utility model discloses a fluorescein column chromatography separation device, and belongs to the technical field of chromatography device.The separation device comprises a shelving plate and a base plate arranged at the lower end of the shelving plate, the two ends of the shelving plate are provided with a first assembly plate and a second assembly plate respectively, and reagent tubes are inserted into the shelving plate; an observation mechanism is arranged between the first assembly plate and the second assembly plate, the observation mechanism comprises lifting plates arranged on the first assembly plate and the second assembly plate respectively, and auxiliary arms are fixedly mounted on the lifting plates; the lifting plate is moved to the boundary of the separation layer on the first group of reagent tubes along with the calibration line, and when the subsequent reagent tubes are sequentially inserted on the shelving plate, the calibration line can distinguish the boundary difference on each reagent tube according to the boundary of the first group of reagent tubes, so that compared with the traditional visual inspection, the calibration line has the advantages that the accuracy is high; according to the scheme, the calibration line is used as a reference, and the difference of separation mixtures in a plurality of groups of reagent tubes can be distinguished more quickly.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of chromatography devices, and particularly relates to a fluorescein column chromatography separation device. BACKGROUND

[0002] Fluorescein column chromatography is commonly used for separating compounds containing a fluorescent group (such as fluorescein, fluorescently labeled molecules, etc.). In the process of column chromatography, the sample interacts with the mobile phase through a solid phase (usually silica gel, alumina, etc. adsorbent material) to achieve the effect of separation. Due to the special fluorescence characteristics of fluorescent compounds, the flow and purification process of the sample can be monitored in real time during the separation process through fluorescence detection.

[0003] The existing fluorescein column chromatography device usually loads the mixture to be separated into a column filled with adsorbent, and separates different components by utilizing the interaction of the components in the stationary phase and the mobile phase. In order to evaluate and confirm the separation effect, the experimental personnel often rely on naked eye observation of the fluorescence reaction between different horizontal layers in the separation process to judge the accuracy of the separation. However, this naked eye observation-based method has the problem of inaccurate separation layer judgment. Since the color change of fluorescein shows a relatively weak difference with the concentration, naked eye observation cannot accurately judge the distribution of different separation layers, especially in the case of low sample concentration or weak fluorescence intensity, the boundary of the separation layer is not easy to determine, and it is easy to cause judgment errors.

[0004] Therefore, the application provides a fluorescein column chromatography separation device to solve the above problems. CONTENT OF THE INVENTION

[0005] The application provides a fluorescein column chromatography separation device, which aims to solve the problem of inaccurate judgment of the distribution of different separation layers due to the relatively weak difference between the color change of fluorescein and the concentration.

[0006] To achieve the above-mentioned purpose, the application provides the following technical scheme: a fluorescein column chromatography separation device, comprising a resting plate and a base plate arranged at the lower end of the resting plate, first and second assembly plates are arranged at the two ends of the resting plate respectively, and a reagent tube is inserted into the resting plate;

[0007] An observation mechanism is arranged between the first and second assembly plates.

[0008] The observation mechanism comprises lifting plates arranged on the first assembly plate and the second assembly plate respectively, and a vice arm is fixedly installed on each lifting plate, and a calibration line for calibration is installed between the two vice arms; one lifting plate is arranged on the first assembly plate and one lifting plate is arranged on the second assembly plate, and the lifting plates on the first assembly plate and the second assembly plate are used to straighten the calibration line by the vice arms to calibrate the separation layer of the mixed solution.

[0009] Preferably, a bundle rod corresponding to the lifting plate is arranged in the first assembly plate and the second assembly plate, a threaded rod is rotatably connected to the first assembly plate, the lifting plate is sleeved with the threaded rod, and when the height of the lifting plate and the calibration line needs to be adjusted, the threaded rod is rotated by rotating the second bevel gear by hand, and different mixed solution separation layers can be adapted by freely adjusting the height of the calibration line.

[0010] Preferably, a shaft rod is rotatably connected to the side of the first assembly plate away from the vice arm, a second bevel gear is fixedly connected to the end of the shaft rod close to the threaded rod, a first bevel gear for engaging with the second bevel gear is sleeved on the threaded rod, a rotating wheel is installed at the end of the shaft rod away from the threaded rod, and a limiting frame for sleeving the shaft rod is installed in the first assembly plate.

[0011] Preferably, a positioning rod is fixedly installed in the second assembly plate, and the positioning rod is movably inserted with the corresponding lifting plate, so that the positioning rod limits the up-and-down movement of the lifting plate on the second assembly plate, thereby avoiding single-side inclination when moving up and down.

[0012] Preferably, the vice arm and the lifting plate are fixed by a hexagonal screw rod.

[0013] Preferably, a synchronous rod is fixedly installed between the two lifting plates.

[0014] The separation device is characterized in that the resting plate and the base plate are installed between the first assembly plate and the second assembly plate, the lifting plates on the first assembly plate and the second assembly plate are horizontally aligned with each other, the vice arms on the two lifting plates are connected by the calibration line, the calibration line is in a tight state, and when the separation layer difference of multiple reagent tubes needs to be observed, the lifting plate with the calibration line is moved to the boundary line of the separation layer of the first group of reagent tubes, and when the subsequent reagent tubes are sequentially inserted on the resting plate, the calibration line can distinguish the boundary line difference of each reagent tube according to the boundary line of the first group of reagent tubes. Compared with the traditional naked eye observation, the calibration line is used as a reference to distinguish the difference between the separation mixtures in multiple groups of reagent tubes more quickly. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 This is a schematic diagram of a fluorescein column chromatography separation device;

[0016] Figure 2 This is a structural schematic diagram of the back of the first assembly plate;

[0017] Figure 3 This is a structural schematic diagram of the cross-sectional view of the first assembly plate;

[0018] Figure 4 This is a structural schematic diagram of the cross-sectional view of the second assembly plate.

[0019] Figure 5 for Figure 1 Enlarged structural diagram at point A in the middle.

[0020] In the picture:

[0021] 1. Shelf; 11. Base plate; 2. Reagent tube; 3. Observation mechanism; 31. First assembly plate; 311. First helical gear; 32. Second assembly plate; 33. Threaded rod; 34. Rotary wheel; 341. Shaft; 342. Limiting frame; 343. Second helical gear; 35. Binding rod; 36. Lifting plate; 361. Secondary arm; 362. Hexagonal screw; 363. Calibration line; 364. Synchronizing rod; 37. Positioning rod. Detailed Implementation

[0022] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0023] This embodiment provides a fluorescein column chromatography separation device, such as... Figures 1-5 As shown, the separation device includes a shelf plate 1 and a base plate 11 disposed at the lower end of the shelf plate 1. A first assembly plate 31 and a second assembly plate 32 are respectively disposed at both ends of the shelf plate 1. A reagent tube 2 is inserted into the shelf plate 1.

[0024] An observation mechanism 3 is provided between the first assembly plate 31 and the second assembly plate 32;

[0025] The observation mechanism 3 comprises lifting plates 36 arranged on the first assembly plate 31 and the second assembly plate 32 respectively, and a sub-arm 361 is fixedly installed on each lifting plate 36; a calibration line 363 for calibration is installed between the two sub-arms 361; a beam 35 corresponding to the lifting plate 36 is arranged in the interior of the first assembly plate 31 and the second assembly plate 32; a threaded rod 33 is rotatably connected to the interior of the first assembly plate 31, and the lifting plate 36 is sleeved with the threaded rod 33.

[0026] Specifically, the resting plate 1 and the base plate 11 are installed between the first assembly plate 31 and the second assembly plate 32, the through hole formed in the resting plate 1 is mainly used for positioning the reagent tube 2, one lifting plate 36 is arranged on each of the first assembly plate 31 and the second assembly plate 32, and the lifting plate 36 arranged on the first assembly plate 31 and the lifting plate 36 arranged on the second assembly plate 32 are both used for straightening the calibration line 363 by the sub-arm 361 to calibrate the mixed liquid separation layer; the lifting plate 36 is sleeved with the threaded rod 33 by screwing, and the lifting plate 36 moves up and down when the threaded rod 33 rotates.

[0027] The first assembly plate 31 is rotatably connected with a shaft 341 on the side away from the sub-arm 361, a second bevel gear 343 is fixedly connected to the end of the shaft 341 close to the threaded rod 33, a first bevel gear 311 for engaging with the second bevel gear 343 is sleeved on the threaded rod 33, a rotating wheel 34 is installed at the end of the shaft 341 away from the threaded rod 33, and a limiting frame 342 for sleeving the shaft 341 is installed in the interior of the first assembly plate 31.

[0028] More specifically, the rotating wheel 34 on the first assembly plate 31 is connected with the shaft 341 and the second bevel gear 343, when it is necessary to adjust the height of the lifting plate 36 and the calibration line 363, the rotating wheel 34 is manually rotated to rotate the threaded rod 33 through the second bevel gear 343, and the different mixed liquid separation layers can be adapted by freely adjusting the height of the calibration line 363, so that the applicability of the device is more extensive, and the limiting frame 342 limits the shaft 341.

[0029] The second assembly plate 32 is fixedly installed with a positioning rod 37 in the interior, and the positioning rod 37 is movably inserted into the corresponding lifting plate 36.

[0030] It should be noted that the positioning rod 37 limits the up and down movement of the lifting plate 36 on the second assembly plate 32, so as to avoid single side tilting when moving up and down.

[0031] The sub-arm 361 is fixedly connected with the lifting plate 36 through a hexagonal screw rod 362, and a synchronous rod 364 is fixedly installed between the two lifting plates 36.

[0032] It is worth mentioning that the lifting plate 36 and the auxiliary arm 361 are fixed by the internal hexagonal screw rod 362, and the calibration line 363 of different colors can be replaced at any time in the later stage, so as to more intuitively observe the difference of the separation layer of the mixed liquid in multiple groups.

[0033] In use, the rest plate 1 and the base plate 11 are installed between the first assembly plate 31 and the second assembly plate 32, the lifting plate 36 on the first assembly plate 31 is horizontally aligned with the lifting plate 36 on the second assembly plate 32, and the auxiliary arm 361 on the two lifting plates 36 is connected by the calibration line 363, which is in a tight state. When the separation layer difference of the experiment in multiple reagent tubes 2 needs to be observed, the lifting plate 36 with the calibration line 363 only needs to be moved to the boundary line of the separation layer of the first group of reagent tubes 2. When the subsequent reagent tubes 2 are sequentially inserted on the rest plate 1, the calibration line 363 can distinguish the boundary line difference of each reagent tube 2 according to the boundary line of the first group of reagent tubes 2 as a reference. Compared with the traditional naked eye observation, the scheme uses the calibration line 363 as a reference to distinguish the difference of the separation mixture in multiple groups of reagent tubes 2 more quickly.

[0034] The above is only the preferred specific embodiment of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art can make equivalent replacement or change according to the technical scheme and concept of the present application within the technical range disclosed by the present application, which should be covered within the protection scope of the present application.

Claims

1. A fluorescein column chromatography separation device, comprising a rest plate (1) and a base plate (11) arranged at the lower end of the rest plate (1), first and second assembly plates (31, 32) are arranged at the two ends of the rest plate (1), respectively, and a reagent tube (2) is inserted into the rest plate (1); characterized in that An observation mechanism (3) is arranged between the first and second assembly plates (31, 32); The observation mechanism (3) comprises lifting plates (36) arranged on the first and second assembly plates (31, 32), respectively, and a vice arm (361) is fixedly installed on each lifting plate (36), and a calibration line (363) for calibration is installed between the two vice arms (361).

2. The fluorescein column chromatography separation device of claim 1, wherein: A beam rod (35) corresponding to the lifting plate (36) is installed inside the first and second assembly plates (31, 32), a threaded rod (33) is rotatably connected inside the first assembly plate (31), and the lifting plate (36) is sleeved with the threaded rod (33).

3. The fluorescein column chromatography separation device of claim 2, wherein: A shaft rod (341) is rotatably connected to the side of the first assembly plate (31) away from the vice arm (361), a second inclined gear (343) is fixedly connected to one end of the shaft rod (341) close to the threaded rod (33), a first inclined gear (311) meshing with the second inclined gear (343) is sleeved on the threaded rod (33), a rotating wheel (34) is installed at the end of the shaft rod (341) away from the threaded rod (33), and a limiting frame (342) for sleeving the shaft rod (341) is installed inside the first assembly plate (31).

4. The fluorescein column chromatography separation device of claim 3, wherein: A positioning rod (37) is fixedly installed inside the second assembly plate (32), and the positioning rod (37) is movably inserted into the corresponding lifting plate (36).

5. The fluorescein column chromatography separation device of claim 4, wherein: The vice arm (361) and the lifting plate (36) are fixedly inserted by a hexagonal screw rod (362).

6. The fluorescein column chromatography separation device of claim 5, wherein: Synchronous rods (364) are fixedly installed between the two lifting plates (36).

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