Soil microbial biomass measurement pretreatment device
By combining a small sealed container and an explosion-proof stainless steel container, along with a pressure controller and an air pump, the problems of sealing and unstable pressure control in traditional devices are solved, achieving efficient and safe pretreatment for soil microbial biomass determination, and improving sample processing capacity and experimental efficiency.
Patent Information
- Application Number
- CN202423051348.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-10
- Publication Date
- 2025-12-19
- Estimated Expiration
- 2034-12-10
AI Technical Summary
Traditional soil microbial biomass determination pretreatment devices suffer from insufficient sealing performance, unstable air pressure control, and limited sample processing capacity, which affect the accuracy and efficiency of experimental results.
The device, consisting of a small sealed container, an explosion-proof stainless steel container, a pressure controller, and an air pump, combined with a sealing ring and a quick connector, achieves good sealing effect, stable and controllable air pressure, and greatly increases the sample processing capacity.
It improved the safety and accuracy of the experiment, enhanced the stability of the experimental environment, increased the sample processing capacity, shortened the experimental cycle, and improved the experimental efficiency.
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Figure CN223688323U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to soil detection technical field, concretely relates to a soil microbial biomass determination pretreatment device. BACKGROUND
[0002] Soil is composed of rock weathering mineral, organic matter produced by plant and animal and microbial residue decomposition, soil organism (solid phase), moisture (liquid phase), air (gas phase) and oxidized humus. Among them, soil microorganism is the general term of small organisms, including bacteria, archaea, fungi and the like, which is large in quantity and changes with soil environment and depth. They carry out oxidation, nitration, ammoniation, nitrogen fixation, sulfuration and the like in soil, promote organic matter decomposition and nutrient conversion, and are crucial to soil nutrient cycle. In recent years, the research on soil microbial biomass carbon, nitrogen and phosphorus is increasing, and the relevant research results have important guiding significance to agricultural production.
[0003] As the "pool" and "source" of soil active elements, soil microbial biomass carbon, nitrogen and phosphorus directly regulate soil element supply. At present, the commonly used detection method internationally is chloroform fumigation-K2SO4 extraction method. The pretreatment steps of the method include: placing soil in a closed large plastic bucket for 7-15 days, the bucket should have appropriate water to maintain humidity, and a small cup of 1mol / L sodium hydroxide solution is placed in the bucket to absorb carbon dioxide produced by soil respiration, and the culture temperature is 25 DEG C. After the culture is finished, 3 portions of 25.0g (oven-dried base weight) of fresh soil (moisture content is 40% of saturated water holding capacity) are weighed in 50ml beakers, and are fumigated with de-ethanol chloroform, which is placed in a vacuum dryer with a small amount of water (about 200ml) and de-ethanol chloroform (40ml) at the bottom, and the chloroform is added to the beaker, and a piece of porcelain treated with concentrated sulfuric acid (0.5cm in size, explosion-proof) is placed in the beaker. After the chloroform is boiled vigorously for 3-5 minutes under-0.08MPa vacuum degree, the vacuum dryer valve is closed, and the soil is moved to another clean vacuum dryer, and is repeatedly vacuumed (-0.08MPa) for 6 times, 3 minutes each time, so that the chloroform in the soil is completely removed (the residual chloroform in the soil has a great influence on the determination of extracted carbon). At the same time of fumigation, 3 portions of 25.0g (oven-dried base weight) of soil are taken in 125ml extraction bottles (not fumigated for comparison).
[0004] From the above process, it can be seen that the equipment used in the traditional pretreatment step includes a large plastic bucket, a sealing tape, a small beaker, a glass vacuum dryer, vaseline and a gas pump. However, the traditional method has the following disadvantages in the treatment process:
[0005] 1. Insufficient sealing performance: The sealing tape is often difficult to achieve the ideal sealing effect due to its material and pasting method, which may lead to gas exchange during soil cultivation, thereby affecting the accuracy of experimental results;
[0006] 2. Unstable air pressure control: When adjusting the air pressure inside the vacuum dryer, manual adjustment is required. If the air pressure control is not proper or stable, it is easy to cause the glass vacuum dryer to break or even explode due to sudden internal pressure change, which not only endangers the safety of experimental personnel, but also damages experimental equipment and leads to experimental failure;
[0007] 3. Limited sample processing capacity: Due to the limitations of equipment capacity and operation process, the traditional method can only process a small number of samples at the same time, which greatly reduces the experimental efficiency, especially in researches that require a large amount of data support.
[0008] Therefore, we propose a soil microbial biomass determination pretreatment device to solve the above technical problems. Content of the utility model
[0009] In order to solve the technical problems existing in the above-mentioned prior art, the utility model provides a soil microbial biomass determination pretreatment device.
[0010] The technical scheme adopted by the utility model is as follows:
[0011] A soil microbial biomass determination pretreatment device, comprising a culture assembly and a fumigation treatment assembly, the culture assembly comprises a small sealed barrel, the fumigation treatment assembly comprises an explosion-proof stainless steel barrel, an air pressure controller and an air pump, hooks are arranged on the inner side walls of the small sealed barrel and the explosion-proof stainless steel barrel, a hanging cup is hung on the hooks, a first sealing cover and a second sealing cover matched with each other are arranged at the openings of the small sealed barrel and the explosion-proof stainless steel barrel respectively, sealing rings are arranged on the lower sides of the first sealing cover and the second sealing cover, a first connector is communicated with the air pressure controller, a first pipeline, a second pipeline and a safety valve are arranged in communication on the first connector, the first pipeline is communicated with the air pump, and the second pipeline is communicated with the explosion-proof stainless steel barrel.
[0012] In a further technical scheme, a second connector is arranged in penetration on the second sealing cover, the second pipeline is arranged in communication on the second connector, and a first on-off valve is arranged at the connection between the second pipeline and the second connector.
[0013] In a further technical scheme, a first air pressure gauge and a third pipeline are further included, the first air pressure gauge and the third pipeline are arranged in communication on the second connector, and a second on-off valve is arranged at the connection between the third pipeline and the second connector.
[0014] In a further technical solution, vaseline is applied on the sealing ring.
[0015] In a further technical solution, a handle is arranged on the outer sidewall of the explosion-proof stainless steel barrel.
[0016] In a further technical solution, the hanging cup is a disposable plastic cup.
[0017] In a further technical solution, a second air pressure gauge is arranged on the first joint.
[0018] In a further technical solution, the joints of the first joint are quick joints.
[0019] In summary, due to the adoption of the above technical solutions, the beneficial effects of the present application are:
[0020] 1. The present application effectively solves the problems of poor sealing effect and difficult disassembly when a traditional large plastic barrel is used with a sealing tape by using a small sealing barrel and a first sealing cover equipped with a sealing ring.
[0021] 2. The present application uses an explosion-proof stainless steel barrel to replace a traditional glass dryer, which not only enhances safety and light resistance, but also ensures the safety of experimenters and the stability of the experimental environment.
[0022] 3. The present application introduces an air pressure controller to maintain a constant pressure in the explosion-proof stainless steel barrel, which is more intelligent than the traditional manual adjustment method, does not require manual attendance, and greatly improves the accuracy and stability.
[0023] 4. In the present application, the hanging cup can be hung on the inner sidewall of the small sealing barrel and the explosion-proof stainless steel barrel, which effectively improves the processing capacity of soil samples compared to the traditional method of directly placing a beaker on the bottom of the barrel, thereby greatly improving the experimental efficiency and facilitating the progress of the experiment, providing a more convenient and efficient solution for the determination of soil microbial biomass. BRIEF DESCRIPTION OF DRAWINGS
[0024] The present application will be illustrated by examples and with reference to the accompanying drawings, in which:
[0025] Figure 1 is a structural schematic view of the fumigation treatment assembly of the present application;
[0026] Figure 2 is a structural schematic view of the culture assembly of the present application.
[0027] Fig. 1 is a small sealed barrel, 2 is an explosion-proof stainless steel barrel, 3 is a gas pressure controller, 4 is a gas pump, 5 is a hook, 6 is a hanging cup, 7 is a first sealing cover, 8 is a second sealing cover, 9 is a sealing ring, 10 is a first connector, 11 is a first pipeline, 12 is a second pipeline, 13 is a safety valve, 14 is a second connector, 15 is a first on-off valve, 16 is a first air pressure gauge, 17 is a third pipeline, 18 is a second on-off valve, 19 is a handle, 20 is a second air pressure gauge. DETAILED DESCRIPTION
[0028] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.
[0029] Referring to Figure 1 and Figure 2 The present application provides a soil microbial biomass determination pretreatment device, which comprises a culture assembly and a fumigation treatment assembly. The culture assembly comprises a small sealed barrel 1. The fumigation treatment assembly comprises an explosion-proof stainless steel barrel 2, a gas pressure controller 3 and a gas pump 4. Hooks 5 are arranged on the inner side walls of the small sealed barrel 1 and the explosion-proof stainless steel barrel 2. Hanging cups 6 are hung on the hooks 5. First sealing covers 7 and second sealing covers 8 are arranged at the openings of the small sealed barrel 1 and the explosion-proof stainless steel barrel 2, respectively. Sealing rings 9 are arranged at the lower sides of the first sealing covers 7 and the second sealing covers 8. A first connector 10 is connected to the gas pressure controller 3. A first pipeline 11, a second pipeline 12 and a safety valve 13 are arranged on the first connector 10 in communication. The first pipeline 11 is communicated with the gas pump 4. The second pipeline 12 is communicated with the explosion-proof stainless steel barrel 2.
[0030] The device is used for soil microbial biomass determination pretreatment process, mainly around the closed culture of soil samples, fumigation and removal of chloroform. The specific working principle is as follows:
[0031] The small sealed barrel 1 is used as a place for soil sample culture, and the soil sample is placed in the small sealed barrel 1. At the same time, the hanging cup 6 containing 1 mol / L sodium hydroxide solution is hung on the hook 5 in the small sealed barrel 1, which is used to absorb the carbon dioxide generated in the culture process. Then, the first sealing cover 7 with a sealing ring 9 is used to seal the opening of the small sealed barrel 1, so that the soil sample can be stably cultured for 7 to 15 days under suitable temperature and humidity conditions. After the culture is completed, part of the soil sample in the small sealed barrel 1 needs to be taken out for fumigation treatment, and part of the soil sample is taken out as a control group without fumigation treatment. At this time, a small amount of water and de-ethanol chloroform is placed in the bottom of the explosion-proof stainless steel barrel 2, and the soil sample to be fumigated is placed in the explosion-proof stainless steel barrel 2. At the same time, the hanging cup 6 containing chloroform is hung on the hook 5 in the explosion-proof stainless steel barrel 2, and the broken porcelain pieces treated with concentrated sulfuric acid are placed in it to prevent explosion. Then, the second sealing cover 8 is covered, and the sealing ring 9 is also used to ensure the sealing of the barrel. After that, the air pump 4 is started to vacuumize the explosion-proof stainless steel barrel 2, and the pressure in the explosion-proof stainless steel barrel 2 is accurately controlled through the air pressure controller 3 and the safety valve 13, so that it is maintained at a vacuum degree of-0.08 MPa. Under this condition, the chloroform is boiled vigorously for 3 to 5 minutes, and then the soil is fumigated in a dark environment at a suitable temperature for 24 hours. Finally, the soil sample is transferred to another explosion-proof stainless steel barrel 2, and the air pump 4 is started again for vacuumization (-0.08 MPa vacuum degree), and the process is repeated for 6 times, each time for 3 minutes, to ensure that the chloroform in the soil is completely removed, so as to avoid the influence of residual chloroform in the soil on the subsequent determination of carbon extraction. Compared with the traditional pretreatment step, first of all, by using the small sealed barrel 1 and the first sealing cover 7 with a sealing ring 9, the problem of poor sealing effect and difficult disassembly when the traditional large plastic barrel and sealing tape are used is effectively solved. Secondly, the explosion-proof stainless steel barrel 2 is used instead of the traditional glass dryer, which not only enhances the safety and light-proof performance, but also ensures the safety of the experimental personnel and the stability of the experimental environment. More importantly, the introduction of the air pressure controller 3 makes the pressure in the explosion-proof stainless steel barrel 2 maintain constant, which is more intelligent than the traditional manual adjustment method, does not need manual attendance, and the accuracy and stability are greatly improved. In addition, the hanging cup 6 can be hung on the inner side wall of the small sealed barrel 1 and the explosion-proof stainless steel barrel 2, which effectively improves the processing capacity of the soil sample compared with the traditional method of directly placing the beaker at the bottom of the barrel, thereby greatly improving the experimental efficiency and increasing the experimental progress, and providing a more convenient and efficient solution for the determination of soil microbial biomass.
[0032] In a specific embodiment, referring to Figure 1The second sealing cover 8 is provided with a second joint 14, and the second pipeline 12 is connected to the second joint 14, and the second pipeline 12 is provided with a first on-off valve 15 at the connection position of the second joint 14.
[0033] The second pipeline 12 is detachably connected to the explosion-proof stainless steel barrel 2 through the second joint 14, which improves the operation convenience of the soil microbial biomass determination pretreatment process. Specifically, during the fumigation treatment process, when the explosion-proof stainless steel barrel 2 needs to be moved, the first on-off valve 15 is closed, and the second pipeline 12 and the second joint 14 are easily disconnected, so that the movement of the explosion-proof stainless steel barrel 2 is not limited. After the fumigation treatment is completed, another explosion-proof stainless steel barrel 2 can be directly connected to the second pipeline 12 through the second joint 14. Due to the asynchronization of the time required for fumigation treatment and removal of chloroform, the experimental personnel can fully utilize the time difference to flexibly arrange multiple explosion-proof stainless steel barrels 2 for parallel processing, which can greatly improve the processing capacity of soil samples, effectively shorten the entire experimental period, and promote the efficient promotion of soil microbial biomass determination work.
[0034] In a specific embodiment, referring to Figure 1 , the first gas pressure gauge 16 and the third pipeline 17 are both connected to the second joint 14, and the third pipeline 17 is provided with a second on-off valve 18 at the connection position of the second joint 14.
[0035] The first gas pressure gauge 16 can monitor the gas pressure in the explosion-proof stainless steel barrel 2 in real time, ensure that the required vacuum degree is stably maintained during the experiment, and thus significantly improve the accuracy and reliability of the experimental results. In addition, the design of the third pipeline 17 and the second on-off valve 18 can quickly fill air into the explosion-proof stainless steel barrel 2 through the third pipeline 17 by opening the second on-off valve 18 after the fumigation treatment or chloroform removal step is completed, thereby facilitating the opening of the explosion-proof stainless steel barrel 2.
[0036] In a specific embodiment, the sealing ring 9 is smeared with vaseline.
[0037] By smearing vaseline on the sealing ring 9, it is ensured that the sealing ring 9 can be more closely and effectively attached to the barrel wall, the sealing effect is enhanced, gas leakage is prevented, the accuracy of the experimental process is ensured, and the accuracy and reliability of the experimental results are ensured.
[0038] In a specific embodiment, referring to Figure 1 , the outer wall of the explosion-proof stainless steel barrel 2 is provided with a handle 19.
[0039] The handle 19 is arranged to enable the experimenter to carry the explosion-proof stainless steel barrel 2 more easily, reduce the difficulty of the carrying process, and improve work efficiency.
[0040] In a specific embodiment, the cup 6 is a disposable plastic cup.
[0041] The cup 6 is a disposable plastic cup, which can meet the storage requirements of sodium hydroxide solution and chloroform, and has low cost. Since it is disposable, it does not need to be cleaned and disinfected, greatly simplifying the cleaning process after the experiment and effectively saving the time and energy of the experimenter.
[0042] In a specific embodiment, referring to Figure 1 , the first joint 10 is provided with a second air pressure gauge 20.
[0043] The second air pressure gauge 20 can monitor the air pressure state in real time, which helps to discover and solve potential air pressure abnormal problems in time, thereby avoiding possible experimental accidents caused by improper air pressure and improving the safety of the experimental process.
[0044] In a specific embodiment, the joints of the first joint 10 are quick joints.
[0045] The use of quick joints improves the convenience of pipeline connection and disconnection, not only simplifies maintenance and replacement work, but also promotes the quick storage and arrangement of the device.
[0046] The above only describes the embodiments of the present application, and does not limit the patent range of the present application, and any equivalent structure or equivalent process conversion using the content of the present application specification and drawings, or direct or indirect application in other related technical fields, are also included in the patent protection range of the present application.
Claims
1. A soil microbial biomass determination pre-treatment device, characterized by, The utility model provides a kind of fumigation and sterilization device, including culture component and fumigation and sterilization processing component, the culture component includes small sealed barrel (1), the fumigation and sterilization processing component includes explosion-proof stainless steel barrel (2), air pressure controller (3) and air pump (4), and the inner side wall of small sealed barrel (1) and explosion-proof stainless steel barrel (2) is equipped with hook (5), cup (6) is hung on the hook (5), and the opening of small sealed barrel (1) and explosion-proof stainless steel barrel (2) is equipped with the first sealing cover (7) and second sealing cover (8) respectively, and the lower side of the first sealing cover (7) and second sealing cover (8) is equipped with sealing ring (9), and the first joint (10) is communicated on air pressure controller (3), and the first joint (10) is equipped with the first pipeline (11), second pipeline (12) and safety valve (13) in communication, and the first pipeline (11) is communicated with air pump (4), and the second pipeline (12) is communicated with explosion-proof stainless steel barrel (2).
2. A device for the pre-treatment of soil microbial biomass determination according to claim 1, characterized in that, Second joint (14) is provided on the second sealing cover (8), and the second pipeline (12) is communicated on the second joint (14), and the first open-close valve (15) is provided on the second pipeline (12) at the connection with the second joint (14).
3. A device for the pre-treatment of soil microbial biomass determination according to claim 2, characterised in that, First air pressure gauge (16) and third pipeline (17) are further included, and the first air pressure gauge (16) and third pipeline (17) are communicated on the second joint (14), and the second open-close valve (18) is provided on the third pipeline (17) at the connection with the second joint (14).
4. The apparatus for the pretreatment of soil microbial biomass determination according to claim 1, characterized in that, The sealing ring (9) is smeared with vaseline.
5. The apparatus for the pretreatment of soil microbial biomass determination according to claim 1, characterized in that, The outer side wall of the explosion-proof stainless steel barrel (2) is provided with a handle (19).
6. A device for the pretreatment of soil microbial biomass determination according to claim 1, characterized in that, The cup (6) is a disposable plastic cup.
7. A device for the pretreatment of soil microbial biomass determination according to claim 1, characterized in that, The first joint (10) is provided with a second air pressure gauge (20).
8. A device for the pretreatment of soil microbial biomass determination according to claim 1, characterized in that, The joint of the first joint (10) is a quick joint.