Cracking device for nucleic acid extraction equipment and nucleic acid extraction equipment

Abstract

The utility model discloses a cracking device for nucleic acid extraction equipment and the nucleic acid extraction equipment, the cracking device comprises a reagent tube carrier used for placing reagent tubes, and a probe inlet / outlet is formed in the vertical side wall of the reagent tube carrier; the ultrasonic mechanism is telescopically arranged on one side of the reagent tube carrying frame and is provided with an ultrasonic probe, the ultrasonic probe can penetrate through the probe inlet and outlet and output ultrasonic waves to a reagent in the reagent tube when the ultrasonic mechanism is in an extension state, and an arc-shaped wall used for being attached to the peripheral wall of the reagent tube is formed on the side, facing the reagent tube, of the ultrasonic probe. The cracking device for the nucleic acid extraction equipment and the nucleic acid extraction equipment have the advantages that the structure is simple, and the ultrasonic cracking efficiency of a reagent can be improved.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of nucleic acid extraction equipment, and particularly relates to a lysis device for a nucleic acid extraction equipment and the nucleic acid extraction equipment. BACKGROUND

[0002] The nucleic acid extraction equipment (such as a nucleic acid extraction instrument) belongs to the field of molecular detection equipment and is widely applied in various fields such as disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial detection, food safety detection, animal husbandry and molecular biology research. Before the nucleic acid extraction equipment performs nucleic acid extraction, a lysis device is used to perform ultrasonic lysis operation on reagents in a reagent tube, so as to reduce the adverse effects on the internal substances of cells in the reagents. However, the lysis device of the existing nucleic acid extraction equipment has a relatively low ultrasonic lysis efficiency due to an unreasonable structure design. CONTENT OF THE UTILITY MODEL

[0003] The application aims to provide a lysis device for a nucleic acid extraction equipment and the nucleic acid extraction equipment, which have the advantages of simple structure and improved ultrasonic lysis efficiency of reagents.

[0004] To achieve the above-mentioned purpose, the first aspect of the application provides a lysis device for a nucleic acid extraction equipment, which comprises:

[0005] A reagent tube carrier for placing a reagent tube, a probe access opening being formed on a vertical side wall of the reagent tube carrier;

[0006] An ultrasonic mechanism telescopically arranged on one side of the reagent tube carrier and having an ultrasonic probe, the ultrasonic probe being capable of outputting ultrasonic waves to reagents in the reagent tube through the probe access opening in an elongated state of the ultrasonic mechanism, and an arc-shaped wall for abutting with an outer peripheral wall of the reagent tube being formed on a side of the ultrasonic probe facing the reagent tube.

[0007] In the embodiments of the application, the ultrasonic mechanism comprises:

[0008] A probe mounting seat arranged on one side of the reagent tube carrier, the ultrasonic probe being arranged on the probe mounting seat and facing the reagent tube carrier;

[0009] A driving connecting rod penetrating through the probe mounting seat and threadedly connected with the probe mounting seat;

[0010] A driving member drivingly connected with the driving connecting rod.

[0011] In the embodiments of the application, the ultrasonic mechanism further comprises an elastic buffer assembly arranged on the probe mounting seat and used for buffering the ultrasonic probe.

[0012] In the embodiment of the present application, the probe mounting seat is formed with a mounting space, the mounting space is formed with a mounting groove on the side wall facing the reagent tube, and the elastic buffer assembly comprises:

[0013] The first connecting rod is inserted on the probe mounting seat and passes through the mounting space.

[0014] The second connecting rod is horizontally spaced apart from the first connecting rod, is inserted on the probe mounting seat and passes through the mounting space.

[0015] The connecting plate is movably arranged on the first connecting rod and the second connecting rod and located in the mounting space, and the end of the ultrasonic probe away from the reagent tube sequentially passes through the mounting groove, the connecting plate and extends in the direction away from the reagent tube.

[0016] The first elastic member is sleeved on the outside of the first connecting rod, one end of the first elastic member abuts against the connecting plate, and the other end of the first elastic member abuts against the side wall of the mounting space away from the reagent tube.

[0017] The second elastic member is sleeved on the outside of the second connecting rod, one end of the second elastic member abuts against the connecting plate, and the other end of the second elastic member abuts against the side wall of the mounting space away from the reagent tube.

[0018] In the embodiment of the present application, the reagent tube comprises a cylindrical portion and an arcuate portion located at the bottom end of the cylindrical portion, and in the vertical direction, the setting position of the ultrasonic probe corresponds to the position of the cylindrical portion close to the arcuate portion.

[0019] In the embodiment of the present application, the lysis device further comprises a moving guide rail, the extension direction of the moving guide rail and the setting direction of the ultrasonic probe are perpendicular to each other, the reagent tube carrier is movably arranged on the moving guide rail, the number of reagent tubes is multiple, and the multiple reagent tubes are arranged on the reagent tube carrier and distributed along the extension direction of the moving guide rail.

[0020] In the embodiment of the present application, the lysis device further comprises a clamping assembly arranged on the reagent tube carrier and used for clamping the reagent tube.

[0021] In the embodiment of the present application, the reagent tube carrier is formed with a containing space for containing the reagent tube, and the lysis device further comprises a heating member arranged at the bottom of the containing space and used for heating the reagent.

[0022] In the embodiment of the present application, the heating member comprises a base portion and a stop portion, the stop portion is arranged at the top of the base portion and can apply a lateral stop action force to the reagent tube.

[0023] The second aspect of the present application provides a nucleic acid extraction device, which comprises the above-mentioned lysis device for the nucleic acid extraction device.

[0024] According to the technical scheme, the cracking device comprises a reagent tube carrier and an ultrasonic mechanism. The reagent tube carrier is used for placing a reagent tube. A probe access opening is formed on a vertical side wall of the reagent tube carrier. The ultrasonic mechanism is telescopically arranged on one side of the reagent tube carrier and has an ultrasonic probe. In an extended state of the ultrasonic mechanism, the ultrasonic probe can pass through the probe access opening and output ultrasonic waves to the reagent in the reagent tube. An arc-shaped wall for abutting the outer peripheral wall of the reagent tube is formed on the side of the ultrasonic probe facing the reagent tube. The ultrasonic probe abuts the reagent tube through the arc-shaped wall, so that the ultrasonic probe can directly transmit ultrasonic wave energy to the reagent tube through the arc-shaped wall. The reagent tube directly and concentrates receives the ultrasonic wave energy at the position in contact with the arc-shaped wall. The above-mentioned ultrasonic wave energy is focused at the position corresponding to the arc-shaped wall in the reagent tube and is transmitted to the reagent with higher efficiency. The intensity of the ultrasonic wave energy received by the reagent is improved, and the efficiency of ultrasonic lysis of cells in the reagent is improved.

[0025] Other features and advantages of the embodiments of the present application will be described in detail in the following detailed description. BRIEF DESCRIPTION OF DRAWINGS

[0026] The accompanying drawings are included to provide a further understanding of the embodiments of the present application, and constitute a part of the specification, and are used to explain the embodiments of the present application together with the following detailed description. The drawings are not intended to limit the embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor on the basis of the structures shown in the drawings. In the drawings:

[0027] Figure 1 It is a first partial schematic view of the cracking device in the embodiments of the present application.

[0028] Figure 2 It is a partial structural schematic view of the ultrasonic mechanism in the embodiments of the present application.

[0029] Figure 3 It is a second partial schematic view of the cracking device in the embodiments of the present application.

[0030] Figure 4 It is a structural schematic view of the nucleic acid extraction device in the embodiments of the present application.

[0031] Figure 5 It is a structural schematic view of the probe mounting seat in the embodiments of the present application.

[0032] Figure 6 It is a structural schematic view of the reagent tube carrier in the embodiments of the present application.

[0033] Explanation of reference signs

[0034] 1-reagent tube carrier; 101-probe access port; 102-accommodation space; 103-first relief gap; 104-second relief gap; 105-first partition; 106-second partition; 2-reagent tube; 201-cylindrical portion; 202-arcuate portion; 3-ultrasonic mechanism; 301-ultrasonic probe; 302-arcuate wall; 303-probe mounting seat; 304-drive connecting rod; 305-drive member; 306-mounting space; 307-mounting groove; 308-connection portion; 309-mounting portion; 4-elastic buffer assembly; 401-first connecting rod; 402-connection plate; 403-first elastic member; 5-moving guide rail; 6-clamping assembly; 601-first clamping member; 602-second clamping member; 603-first connecting member; 604-second connecting member; 7-heating member; 701-base portion; 702-stop portion; 8-driving mechanism. DETAILED DESCRIPTION

[0035] The specific embodiments of the present application are described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are merely illustrative and explanatory and are not intended to limit the present application.

[0036] Embodiments of the present application provide a lysis device for a nucleic acid extraction apparatus, as shown in Figures 1-3 The lysis device comprises:

[0037] A reagent tube carrier 1 for placing a reagent tube 2, a vertical side wall of the reagent tube carrier 1 is formed with a probe access port 101.

[0038] An ultrasonic mechanism 3 is telescopically arranged on one side of the reagent tube carrier 1 and has an ultrasonic probe 301, in the extended state of the ultrasonic mechanism 3, the ultrasonic probe 301 can pass through the probe access port 101 and output ultrasonic waves to the reagent in the reagent tube 2, one side of the ultrasonic probe 301 facing the reagent tube 2 is formed with an arcuate wall 302 for abutting the outer peripheral wall of the reagent tube 2.

[0039] Specifically, the nucleic acid extraction device in the embodiment can be a nucleic acid extractor. The reagent tube carrier 1 is provided with accommodation spaces 102 recessed from the top and used for accommodating reagent tubes 2. The reagent tubes 2 are placed in the accommodation spaces 102. The number of the reagent tubes 2 is multiple. Different types of solutions (such as mixed solutions of lysing solution and samples, buffer solution, eluent, etc.) are loaded in the multiple reagent tubes 2. Before the ultrasonic mechanism 3 performs the ultrasonic lysis operation, the ultrasonic probe 301 is aligned with the reagent tube 2 containing the reagent (such as the mixed solution of lysing solution and sample, and the sample containing cells) that needs to be lysed. Then the ultrasonic mechanism 3 is controlled to be elongated, so that the ultrasonic probe 301 moves towards the direction where the reagent tube 2 is located. Until the arc-shaped wall 302 on the ultrasonic probe 301 and the outer peripheral wall of the reagent tube 2 are attached. After the ultrasonic mechanism is opened, the ultrasonic probe 301 outputs ultrasonic waves to the reagent tube 2. The reagent tube 2 transmits the ultrasonic wave energy to the reagent in the reagent tube 2. The high-frequency acoustic energy of the above-mentioned ultrasonic waves can break the cells to make the substances (such as nucleic acid) in the cells flow out, which lays the foundation for subsequent nucleic acid extraction. Since the ultrasonic probe 301 is attached to the reagent tube 2 through the arc-shaped wall 302, the contact area between the two is large (compared with the point contact form between the ultrasonic probe 301 and the reagent tube 2). Therefore, the ultrasonic probe 301 can directly transmit ultrasonic wave energy to the reagent tube 2 through the arc-shaped wall 302. The reagent tube 2 directly and concentrates receives the above-mentioned ultrasonic wave energy at the position where the reagent tube 2 contacts the arc-shaped wall 302. That is, the above-mentioned ultrasonic wave energy is focused at the position corresponding to the arc-shaped wall 302 in the reagent tube 2 and is transmitted to the reagent with higher efficiency. The intensity of the ultrasonic wave energy received by the reagent is improved, and the efficiency of ultrasonic lysis of cells in the reagent is improved.

[0040] In an embodiment of the present application, the ultrasonic mechanism 3 comprises:

[0041] The probe mounting seat 303 is arranged on one side of the reagent tube carrier 1. The ultrasonic probe 301 is arranged on the probe mounting seat 303 and faces the reagent tube carrier 1.

[0042] The driving connecting rod 304 is arranged on the probe mounting seat 303 and is in threaded connection with the probe mounting seat 303.

[0043] The driving member 305 is in driving connection with the driving connecting rod 304.

[0044] Specifically, the probe mounting seat 303 comprises a connecting portion 308 and a mounting portion 309 arranged at the bottom end of the connecting portion 308, the ultrasonic probe 301 is mounted on the mounting portion 309, the driving member 305 can be an electric motor, the driving connecting rod 304 is connected with the driving end of the driving member 305, the driving connecting rod 304 is arranged in the same direction as the moving direction of the ultrasonic probe 301, and the connecting portion 308 is sleeved on the driving connecting rod 304 and threadedly connected with the driving connecting rod 304. When the motor rotates, the driving connecting rod 304 is driven to rotate, the connecting portion 308 moves along the axial direction of the driving connecting rod 304, the connecting portion 308 drives the mounting portion 309 and the ultrasonic probe 301 connected with the mounting portion 309 to move when the connecting portion 308 moves, so that the ultrasonic probe 301 approaches or moves away from the reagent tube 2, so as to perform ultrasonic lysis on the reagent in the reagent tube 2.

[0045] In an embodiment of the present application, the ultrasonic mechanism 3 further comprises an elastic buffer assembly 4 arranged on the probe mounting seat 303 and used for buffering the ultrasonic probe 301, the elastic buffer assembly 4 can buffer the impact between the ultrasonic probe 301 and the reagent tube 2 when the driving member 305 fails to drive or the ultrasonic probe 301 has a moving error, so as to avoid rigid impact between the ultrasonic probe 301 and the reagent tube 2, reduce the vibration of the ultrasonic probe 301 and / or the reagent tube 2, protect the ultrasonic mechanism 3, and prolong the service life of the ultrasonic mechanism 3 and the lysis device.

[0046] In an embodiment of the present application, as shown in Figure 5 The probe mounting seat 303 is formed with a mounting space 306, the mounting space 306 is formed with a mounting groove 307 on the side wall facing the reagent tube 2, and the elastic buffer assembly 4 comprises:

[0047] A first connecting rod 401 is inserted on the probe mounting seat 303 and passes through the mounting space 306;

[0048] A second connecting rod is horizontally and spacedly arranged with the first connecting rod 401, the second connecting rod is inserted on the probe mounting seat 303 and passes through the mounting space 306;

[0049] A connecting plate 402 is movably arranged on the first connecting rod 401 and the second connecting rod and located in the mounting space 306, one end of the ultrasonic probe 301 away from the reagent tube 2 passes through the mounting groove 307 and the connecting plate 402 in sequence and extends away from the reagent tube 2;

[0050] A first elastic member 403 is sleeved on the outside of the first connecting rod 401, one end of the first elastic member 403 abuts against the connecting plate 402, and the other end of the first elastic member 403 abuts against the side wall of the mounting space 306 away from the reagent tube 2;

[0051] The second elastic member is sleeved outside the second connecting rod, one end of the second elastic member abuts against the connecting plate 402, and the other end of the second elastic member abuts against the side wall of the mounting space 306 away from the reagent tube 2.

[0052] Specifically, the mounting space 306 is formed on the mounting portion 309, the first connecting rod 401 and the second connecting rod are respectively located on two sides of the ultrasonic probe 301, the first end of the first connecting rod 401 is clamped on the side wall of the mounting space 306 close to the reagent tube 2, and the second end of the first connecting rod 401 sequentially passes through the side wall of the mounting space 306 close to the reagent tube 2, the connecting plate 402 and the side wall of the mounting space 306 away from the reagent tube 2; the first end of the second connecting rod is clamped on the side wall of the mounting space 306 close to the reagent tube 2, and the second end of the second connecting rod sequentially passes through the side wall of the mounting space 306 close to the reagent tube 2, the connecting plate 402 and the side wall of the mounting space 306 away from the reagent tube 2; the connecting plate 402 is sleeved outside the ultrasonic probe 301 and is tightly connected with the ultrasonic probe 301 together, the connecting plate 402 and the ultrasonic probe 301 are two integrals and can move along the axial direction of the first connecting rod 401 and the second connecting rod, and the first elastic member 403 and the second elastic member can be selected as springs. If the ultrasonic probe 301 moves by mistake and collides with the reagent tube 2, the ultrasonic probe 301 can move together with the connecting plate 402 in a direction away from the reagent tube 2, at this time, the first elastic member 403 and the second elastic member are compressed and absorb the impact energy between the ultrasonic probe 301 and the reagent tube 2, so as to avoid the impact energy from being continuously transmitted to cause damage to other parts of the ultrasonic mechanism 3.

[0053] In an embodiment of the present application, the reagent tube 2 includes a cylindrical portion 201 and an arc surface portion 202 located at the bottom end of the cylindrical portion 201, and in the vertical direction, the setting position of the ultrasonic probe 301 corresponds to the position of the cylindrical portion 201 close to the arc surface portion 202, which can make the ultrasonic energy transmitted by the ultrasonic probe 301 act on the reagent as much as possible when the reagent in the reagent tube 2 is less, instead of being first transmitted to the position of the reagent tube 2 without reagent coverage and then transmitted to the position with reagent coverage by the reagent tube 2 (for example, if the liquid level of the reagent in the reagent tube 2 is low and the position of the ultrasonic probe 301 is higher than the liquid level of the reagent, the ultrasonic energy is transmitted to the position of the reagent tube 2 corresponding to the ultrasonic probe 301, and then the reagent tube 2 needs to transmit the ultrasonic energy downward to act on the reagent), which is beneficial to reducing the loss of ultrasonic energy.

[0054] In an embodiment of the present application, as shown in FIG. 4, the ultrasonic mechanism 3 further includes a first elastic member 403 sleeved outside the first connecting rod 401, one end of the first elastic member 403 abuts against the connecting plate 402, and the other end of the first elastic member 403 abuts against the side wall of the mounting space 306 close to the reagent tube 2. Figure 4As shown, the lysis device further comprises a moving guide rail 5, an extension direction of the moving guide rail 5 and a setting direction of the ultrasonic probe 301 are perpendicular to each other, the reagent tube carrier 1 is movably arranged on the moving guide rail 5, the reagent tubes 2 are multiple in number, and the multiple reagent tubes 2 are all arranged on the reagent tube carrier 1 and distributed along the extension direction of the moving guide rail 5.

[0055] Specifically, the nucleic acid extraction device further comprises a warehouse body (not shown in the figure), the moving guide rail 5 is arranged inside the warehouse body, the nucleic acid extraction device further comprises a driving mechanism 8 which is drivingly connected with the reagent tube carrier 1, under the driving action of the driving mechanism 8, the reagent tube carrier 1 can move along the extension direction of the moving guide rail 5, if the reagents in the multiple reagent tubes 2 need to be ultrasonically lysed, after the reagent in one reagent tube 2 is ultrasonically lysed, the reagent tube carrier 1 is driven to move, so that the next reagent tube 2 containing the reagent to be ultrasonically lysed is aligned with the ultrasonic probe 301, without arranging multiple ultrasonic probes 301 to ultrasonically lyse the reagents in the multiple reagent tubes 2 one by one, the structure of the ultrasonic mechanism 3 is simplified, the volume of the ultrasonic mechanism 3 is reduced, and it is also beneficial to reduce the production and manufacturing cost of the lysis device and the nucleic acid extraction device. Further, the multiple reagent tubes 2 in the embodiment are integrally formed, that is, the multiple reagent tubes 2 are integrated into a reagent strip.

[0056] In an embodiment of the present application, the lysis device further comprises a clamping assembly 6 arranged on the reagent tube carrier 1 and used for clamping the reagent tube 2.

[0057] Specifically, as Figures 3-6As shown, one side of the reagent tube carrier 1 is formed with a first avoiding gap 103, the other side of the reagent tube carrier 1 is formed with a second avoiding gap 104 opposite to the first avoiding gap 103, the first avoiding gap 103 and the second avoiding gap 104 both penetrate along the length direction of the reagent tube carrier 1 and both communicate with the accommodating space 102, the reagent tube carrier 1 has a first partition 105 between the first end of the first avoiding gap 103 and the first end of the second avoiding gap 104, and has a second partition 106 between the second end of the first avoiding gap 103 and the second end of the second avoiding gap 104. The clamping assembly 6 includes a first clamping piece 601, a second clamping piece 602, a first connecting piece 603, a second connecting piece 604, a third elastic piece (not shown in the figure) and a fourth elastic piece (not shown in the figure), the first clamping piece 601 is arranged at the position of the first avoiding gap 103, the second clamping piece 602 is arranged at the position of the second avoiding gap 104, one end of the first connecting piece 603 is connected with the first clamping piece 601, the other end of the first connecting piece 603 (such as a bolt) penetrates through the first partition 105 and is connected with the second clamping piece 602, one end of the second connecting piece 604 (such as a bolt) is connected with the first clamping piece 601, the other end of the second connecting piece 604 penetrates through the second partition 106 and is connected with the second clamping piece 602, the accommodating space 102 is between the first clamping piece 601, the second clamping piece 602, the first partition 105 and the second partition 106; the third elastic piece can be a spring and is sleeved outside the first connecting piece 603, one end of the third elastic piece abuts against the first clamping piece 601, the other end of the third elastic piece abuts against the first partition 105, the fourth elastic piece can be a spring and is sleeved outside the second connecting piece 604, one end of the fourth elastic piece abuts against the first clamping piece 601, the other end of the fourth elastic piece abuts against the second partition 106, when the third elastic piece and the fourth elastic piece are compressed or restored to the original state, the first clamping piece 601, the second clamping piece 602, the first connecting piece 603 and the second connecting piece 604 can move as a whole relative to the reagent tube carrier 1 (the moving direction is the width direction of the reagent tube carrier 1).

[0058] Further, a guide wall for guiding the reagent tube 2 (or reagent strip) is formed on the side wall of the accommodation space 102 close to the second clamping piece 602. When the operator installs the reagent tube 2 (or reagent strip), the reagent tube 2 (or reagent strip) can more easily enter the accommodation space 102 under the guidance of the guide wall. Then the operator continues to apply a downward force to the reagent tube 2 (or reagent strip). Under the action of the force, the reagent tube 2 (or reagent strip) continues to move downward. In the process of the downward movement of the reagent tube 2 (or reagent strip), a pushing force is applied to the second clamping piece 602 away from the first clamping piece 601. The first clamping piece 601, the second clamping piece 602, the first connecting piece 603 and the second connecting piece 604 move as a whole towards the direction in which the second clamping piece 602 is located. The third elastic piece and the fourth elastic piece are compressed. After the reagent tube 2 (or reagent strip) is moved into position, the second clamping piece 602 continuously applies a pushing force to the reagent tube 2 (or reagent strip) under the elastic force of the third elastic piece and the fourth elastic piece. The second clamping piece 602 and the vertical side wall of the accommodation space 102 away from the second clamping piece 602 jointly clamp the reagent tube 2 (or reagent strip) to further enhance the stability of the reagent tube 2 (or reagent strip) after installation, which is conducive to further improving the lysis effect on the reagent.

[0059] When the reagent tube 2 (or reagent strip) needs to be taken out, the operator applies a pushing force to the first clamping piece 601 towards the second clamping piece 602, further compresses the third elastic piece and the fourth elastic piece to make the first clamping piece 601, the second clamping piece 602, the first connecting piece 603 and the second connecting piece 604 move as a whole towards the direction in which the second clamping piece 602 is located, to release the clamping action of the second clamping piece 602 and the vertical side wall of the accommodation space 102 away from the second clamping piece 602 on the reagent tube 2 (or reagent strip). Then an upward force is applied to the reagent tube 2 (or reagent strip) to take out the reagent tube 2 (or reagent strip), so that the reagent tube 2 (or reagent strip) can be conveniently taken out, improving the use convenience and reliability of the lysis device.

[0060] In an embodiment of the present application, the reagent tube carrier 1 is formed with an accommodation space 102 for accommodating the reagent tube 2. The lysis device further comprises a heating piece 7 arranged at the bottom of the accommodation space 102 and used for heating the reagent in the reagent tube 2.

[0061] Specifically, after the reagent tube 2 is placed in position, the heating member 7 can heat the reagent (such as a mixture of a lysis solution and a sample) in the reagent tube 2, which helps to release nucleic acid from cells in the sample more quickly, laying a foundation for subsequent nucleic acid extraction. In this embodiment, the heating member 7 is arranged at the bottom of the accommodation space 102 of the reagent tube carrier 1, which can heat the reagent, improve the efficiency of ultrasonic lysis, and individually heat and lyse the reagent according to actual needs. In addition, the integration design of the reagent tube carrier 1 and the heating member 7 improves the space utilization of the accommodation space 102, reduces the overall space occupied by the reagent tube carrier 1 and the heating member 7, is conducive to reducing the size of the lysis device and the nucleic acid extraction equipment, and is conducive to realizing the miniaturization design of the lysis device and the nucleic acid extraction equipment, so that the nucleic acid extraction equipment can be directly placed in a biological safety cabinet.

[0062] In an embodiment of the present application, the heating member 7 includes a base portion 701 and a stop portion 702, and the stop portion 702 is arranged at the top of the base portion 701 and can exert a lateral stop force on the reagent tube 2.

[0063] Specifically, the base portion 701 is formed with an accommodation groove for accommodating the arc surface portion 202 of part of the reagent tube 2, and the ultrasonic probe 301 of the ultrasonic mechanism 3 exerts a pushing force on the reagent tube 2 from the side away from the stop portion 702. When the ultrasonic mechanism 3 performs ultrasonic lysis operation, the stop portion 702 plays a stopping role on the reagent tube 2, and the pushing force exerted by the ultrasonic probe 301 and the lateral stop force are located on opposite radial sides of the reagent tube 2, respectively. Therefore, the arrangement of the stop portion 702 can further enhance the stability of the reagent tube 2 when it is in contact with the ultrasonic probe 301, which is conducive to further improving the effect of ultrasonic lysis.

[0064] Another embodiment of the present application provides a nucleic acid extraction equipment, which includes the lysis device for the nucleic acid extraction equipment in the above-mentioned embodiments.

[0065] In the description of the present application, it should be understood that the terms "first", "second" are only for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first", "second" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "a plurality of" is at least two, such as two, three, etc., unless otherwise specifically limited.

[0066] In this application, unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connecting", "fixing" and the like should be understood in a broad sense, for example, can be fixed connection, can also be detachable connection, or integral; can be mechanical connection, can also be electrical connection or communication with each other; can be directly connected, can also be indirectly connected through an intermediate medium, can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise explicitly limited. For those skilled in the art, the specific meaning of the above terms in this application can be understood according to the specific circumstances.

[0067] In the description of the present specification, the description of the terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. Furthermore, the skilled in the art can combine and combine the different embodiments or examples described in the present specification and the features of the different embodiments or examples without contradiction.

[0068] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limiting the present application, and those skilled in the art can make changes, modifications, replacements and variations to the above embodiments within the scope of the present application.

Claims

1. A lysis device for a nucleic acid extraction apparatus, characterized by, The cracking device comprises: A reagent tube carrier (1) for placing a reagent tube (2), a probe access opening (101) is formed on the vertical side wall of the reagent tube carrier (1); An ultrasonic mechanism (3) is telescopically arranged on one side of the reagent tube carrier (1) and has an ultrasonic probe (301), in the elongated state of the ultrasonic mechanism (3), the ultrasonic probe (301) can pass through the probe access opening (101) and output ultrasonic waves to the reagent in the reagent tube (2), and an arc-shaped wall (302) is formed on the side of the ultrasonic probe (301) facing the reagent tube (2) for abutting with the outer peripheral wall of the reagent tube (2).

2. The lysing apparatus for nucleic acid extraction apparatus according to claim 1, wherein The ultrasonic mechanism (3) comprises: A probe mounting seat (303) is arranged on one side of the reagent tube carrier (1), and the ultrasonic probe (301) is arranged on the probe mounting seat (303) and faces the reagent tube carrier (1); A drive connecting rod (304) is arranged on the probe mounting seat (303) and is in threaded connection with the probe mounting seat (303); A drive member (305) is in driving connection with the drive connecting rod (304).

3. The lysing device for nucleic acid extraction equipment according to claim 2, characterized in that, The ultrasonic mechanism (3) further comprises an elastic buffer assembly (4) arranged on the probe mounting seat (303) and used for buffering the ultrasonic probe (301).

4. The lysing apparatus for nucleic acid extraction equipment according to claim 3, characterized by, An installation space (306) is formed on the probe mounting seat (303), an installation groove (307) is formed on the side wall of the installation space (306) facing the reagent tube (2), and the elastic buffer assembly (4) comprises: A first connecting rod (401) is inserted on the probe mounting seat (303) and passes through the installation space (306); A second connecting rod is horizontally spaced apart from the first connecting rod (401), and the second connecting rod is inserted on the probe mounting seat (303) and passes through the installation space (306); A connecting plate (402) is movably arranged on the first connecting rod (401) and the second connecting rod and located in the installation space (306), one end of the ultrasonic probe (301) away from the reagent tube (2) passes through the installation groove (307) and the connecting plate (402) in sequence and extends away from the reagent tube (2); A first elastic member (403) is sleeved on the outside of the first connecting rod (401), one end of the first elastic member (403) abuts against the connecting plate (402), and the other end of the first elastic member (403) abuts against the side wall of the installation space (306) away from the reagent tube (2); A second elastic member is sleeved on the outside of the second connecting rod, one end of the second elastic member abuts against the connecting plate (402), and the other end of the second elastic member abuts against the side wall of the installation space (306) away from the reagent tube (2).

5. The lysing device for a nucleic acid extraction apparatus according to claim 1, wherein The reagent tube (2) comprises a cylindrical portion (201) and an arc surface portion (202) at the bottom end of the cylindrical portion (201), and the setting position of the ultrasonic probe (301) corresponds to the position of the cylindrical portion (201) close to the arc surface portion (202) in the vertical direction.

6. The lysing device for a nucleic acid extraction apparatus of claim 1, wherein, The lysing device further comprises a moving guide rail (5), the extending direction of the moving guide rail (5) and the setting direction of the ultrasonic probe (301) are perpendicular to each other, and the reagent tube carrier (1) is movably arranged on the moving guide rail (5); the number of the reagent tubes (2) is multiple, and the multiple reagent tubes (2) are arranged on the reagent tube carrier (1) and distributed along the extending direction of the moving guide rail (5).

7. The lysing device for a nucleic acid extraction apparatus of claim 1, wherein, The lysing device further comprises a clamping assembly (6) arranged on the reagent tube carrier (1) and used for clamping the reagent tube (2).

8. The lysing apparatus for nucleic acid extraction equipment according to any one of claims 1 to 7, characterized in that, The reagent tube carrier (1) is formed with a containing space (102) for containing the reagent tube (2), and the lysing device further comprises a heating piece (7) arranged at the bottom of the containing space (102) and used for heating the reagent in the reagent tube (2).

9. The lysing device for nucleic acid extraction equipment according to claim 8, characterized in that, The heating piece (7) comprises a base portion (701) and a stop portion (702), the stop portion (702) is arranged at the top of the base portion (701) and can apply a lateral stop force to the reagent tube (2).

10. A nucleic acid extraction device, characterized by, The nucleic acid extraction device comprises the lysing device for nucleic acid extraction device according to any one of claims 1-9.

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