Aquatic microorganism sampler

Abstract

The utility model relates to an aquatic microorganism sampler which mainly comprises a square-box-shaped artificial matrix frame used for collecting benthonic animals, the bottom surface of the artificial matrix frame is a square iron plate, the four side surfaces of the artificial matrix frame are rectangular iron plates, and high sleeves are embedded in the four corners of the artificial matrix frame; the four iron chisels can penetrate through the high sleeve, pierce into sediment at the water bottom and are used for fixing the water bottom, and the plastic plate is connected with two adjacent iron chisels in a binding mode and is used for collecting periphytic algae. According to the utility model, aquatic organism samples in rivers and lakes can be quantitatively and qualitatively collected by adopting an artificial matrix method, and periphytic algae and benthonic animals can be simultaneously collected; the device is easy to put and take back, is safe and does not cause environmental pollution.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of aquatic microorganism collection, in particular to the equipment for collecting river periphyton and benthic animals by artificial substrate method, and is especially suitable for the current investigation and evaluation of aquatic microbial community. BACKGROUND

[0002] Aquatic animals and microorganisms in natural water bodies play an important role in aquatic ecosystems. The purpose of aquatic microbial monitoring is not only limited to indicating water quality status, i.e. evaluating water environmental quality, but also showing the stability of aquatic ecosystems, i.e. indicating potential aquatic ecological risks. There are two methods for aquatic microbial monitoring, i.e. natural substrate method and artificial substrate method. The natural substrate method has the advantages of on-site collection and the disadvantages of difficulty in quantification, which is suitable for qualitative sample collection. Therefore, when biological samples need to be quantitatively collected, the artificial substrate method is usually used. The artificial substrate method has the advantages of quantitative and qualitative collection of biological samples, and the disadvantages of long period of time in the wild (usually 15 days) and easy loss (being washed away by water or sinking into the bottom mud and being difficult to retrieve).

[0003] Chinese utility model patent CN110495429A discloses a sampler using a cylindrical basket as an artificial substrate. Although the specific surface area is sufficient, the contact area with the river is small, which is not conducive to the dispersion of benthic animal larvae, and the cylindrical basket is not fixed well, which is easy to be washed away by the river (large displacement of the placement site) or sink into the bottom mud and be difficult to retrieve. Chinese utility model patent CN210166159U discloses a cement frame as an artificial substrate, which is mainly used for collecting benthic animals in artificial water channels. Moreover, due to the great difference between the artificial substrate and the natural substrate, it cannot be used for collecting aquatic microorganisms in natural rivers. The utility model uses a square as the bottom, increases the contact area with the river bottom, and is easy to calculate. The iron rod is fixed at a corresponding distance from the shore to avoid being washed away or having a large displacement, which is conducive to the growth of benthic animal larvae. The polyethylene plate is fixed on the iron rod near the water surface, which can collect periphyton and complete the collection of multiple biological samples with one device. The polyethylene plate is water-resistant and corrosion-resistant, which replaces the glass periphyton plate and is not easy to break and lose. Since benthic animal sampling needs to be collected at a certain distance from the shore, the periphyton is correspondingly avoided from being washed to the shore, and the sampling failure is avoided. CONTENT OF THE UTILITY MODEL

[0004] The utility model aims to provide a sampling device for collecting river aquatic microbial samples. The device can simultaneously collect periphyton and benthic animals; the device is simple to operate, low in cost and reusable.

[0005] To achieve the above-mentioned purpose, the utility model provides an aquatic microbial sampler, which uses an artificial substrate method to qualitatively and quantitatively collect aquatic microorganisms, comprising:

[0006] The artificial substrate frame for collecting benthic animals is square box-shaped, the bottom surface is a square iron plate, the four side surfaces are rectangular iron plates, high sleeves are inlaid in the four corners of the artificial substrate frame;

[0007] Four iron rods capable of passing through the high sleeves and penetrating into the water bottom sand for water bottom fixation, and a plastic plate connected with two adjacent iron rods in a binding manner for collecting epiphytic algae.

[0008] Further, the iron plate located at the bottom surface has a size of 50cm*50cm and a thickness of 0.2cm.

[0009] Further, the iron plate located at the side surface has a size of 50cm*10cm and a thickness of 0.2cm.

[0010] Further, the high sleeve has an inner diameter of 2.5cm and a length of 5cm.

[0011] Further, the plastic plate is a high-density polyethylene plate, has a size of 50cm*50cm and a thickness of 0.2cm.

[0012] Further, the iron rod has a blunt tip at one end, has a length of 1.5m and a diameter of 2-2.5cm.

[0013] Further, the iron rod penetrates into the bottom material by a depth of 10-20cm, i.e. the distance between the iron plate of the bottom surface of the artificial substrate frame and the tip of the rod is 10-20cm. BRIEF DESCRIPTION OF DRAWINGS

[0014] Fig. 1 is a structural schematic diagram of the present application, wherein (a) is the front view, (b) is the back view, (c) is the side view, and (d) is the bottom view;

[0015] Figure 2 Fig. 2 is a schematic diagram of the implementation state of the present application.

[0016] In the figure, 1 is the iron rod, 2 is the plastic plate, 3 is the bottom plate, 4 is the side plate, 5 is the high sleeve, and 6 is the substrate (fine sand, gravel or pebble). DETAILED DESCRIPTION

[0017] Example 1

[0018] Referring to Figure 1, the water microorganism sampler provided by the utility model comprises a square box-shaped artificial substrate frame, the square bottom plate 3 is 50cm*50cm, the side plate 4 is 10cm high, the material of the bottom plate and the side plate is all iron plate with a thickness of 0.2cm, the inner diameter of the high sleeve 5 on the side edge is 2.5cm, the high sleeve 5 is 5cm high, the iron rod 1 with a blunt tip is 1.5m long and 2cm in diameter, and there are four iron rods in total; the plastic plate 2 is made of high-density polyethylene plate, is 50cm high, 50cm wide and 0.2cm thick.

[0019] The water microorganism sampler in the river sampling process:

[0020] The water microorganism sampler in the river sampling process as Figure 2 shown, the implementation process is as follows, in the river, taking a straight river channel, a gentle water flow area, placing the water microorganism sampler at a distance of 2m from the river bank (2-5m range is recommended in the low water period, and the distance of the sampler placed in the high water period is greater than 5m operation). Place on both sides of the river, and increase the parallel sampling points as appropriate. The substrate 6 placed in the artificial substrate frame to simulate the actual bottom condition is a fine sand and pebble composite substrate (the substrate 6 can select fine sand, fine gravel or pebble), and the thickness should be less than 10cm. The plastic plate 2 is fixed on the iron rod 1 away from the river bank side, and the height is flat with the river surface (if sampling at the end of the high water period, the plastic plate 2 should be appropriately adjusted to be lower than the river surface), and the plastic plate 2 is embedded into the substrate 10-20cm into the artificial substrate frame for fixation. At this time, the bottom plate 3 of the bottom surface of the artificial substrate frame is 10-20cm away from the steel rod tip, and then the coarse nylon rope is passed through the high sleeve 5 and fixed on the shore to make a mark; the sampler is taken back to the laboratory after 15 days in the retention river, the substrate 6 is screened with a 40 mesh screen, and laboratory biological identification is carried out to obtain qualitative and quantitative data. Next, the periphyton in the area with a 2cm side length of the plastic plate 2 is measured, and the periphyton is scraped into a reagent bottle for identification to obtain quantitative and qualitative data. The parallel samples obtain three groups of 11 families and 16 kinds of benthic animals, and the periphyton covers dozens of algal species in four groups.

[0021] The water microorganism sampler in the river sampling process:

[0022] The water microorganism sampler in the river sampling process as Figure 2 shown, the implementation process is as follows. The water microorganism sampler is placed at a distance of 2m from the river bank. The substrate 6 in the artificial substrate frame is composed of larger pebbles and gravel, and two are placed in parallel. Then, the 1.5m iron rod 1 is inserted into the high sleeve 5, and the high sleeve 5 is fixed on the shore by the nylon rope. The plastic plate 2 is bound on the iron rod 1 in the parallel water flow direction and is immersed in the river as much as possible. After 15 days, the sampler is taken back to the laboratory for identification. Due to the shallow water in the stream, the plastic plate 2 should collect periphyton samples in a certain area in the area with good lower periphyton state.

Claims

1. An aquatic microorganism sampler, characterized in that, it uses an artificial matrix method for qualitative and quantitative collection of aquatic microorganisms, and is further characterized in that, The invention relates to a square box-shaped artificial substrate frame for collecting benthic animals, the bottom surface of which is a square iron plate, the four side surfaces of which are rectangular iron plates, the four corners of the artificial substrate frame being embedded with high sleeves; four iron rods capable of passing through the high sleeves and being inserted into the bottom sediment for underwater fixation, and a plastic plate connected to two adjacent iron rods by binding for collecting epiphytic algae. The iron plate on the bottom surface has a size of 50 cm x 50 cm and a thickness of 0.2 cm.

2. An aquatic microorganism sampler according to claim 1, characterized in that: The iron plate on the side surface has a size of 50 cm x 10 cm and a thickness of 0.2 cm.

3. An aquatic microorganism sampler according to claim 1, wherein: The high sleeve has an inner diameter of 2.5 cm and a length of 5 cm.

4. An aquatic microorganism sampler according to claim 1, wherein: The plastic plate is a high-density polyethylene plate with a size of 50 cm x 50 cm and a thickness of 0.2 cm.

5. An aquatic microorganism sampler according to claim 1, wherein: The iron rod has a blunt tip at one end, a length of 1.5 m and a diameter of 2-2.5 cm.

6. An aquatic microorganism sampler according to claim 1, wherein: The iron rod is inserted into the bottom sediment to a depth of 10-20 cm, i.e. the iron plate on the bottom surface of the artificial substrate frame is 10-20 cm away from the tip of the rod.

7. An aquatic microorganism sampler according to claim 6, wherein: ​

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