Solid-liquid separation device for screening phage single-chain antibody
By designing a solid-liquid separation device that includes a shell, a rotating shaft, a fixed ring, and a driving device, the problems of complicated operation and contamination in the phage screening process are solved, achieving the effects of simplified cleaning and reduced contamination.
Patent Information
- Application Number
- CN202423295161.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2025-12-23
- Estimated Expiration
- 2034-12-31
AI Technical Summary
Existing phage screening processes are cumbersome and prone to contamination, necessitating a closed, semi-automatic solid-liquid separation device to simplify cleaning operations and reduce contamination.
A solid-liquid separation device was designed, comprising a housing, a rotating shaft, a fixed ring, a single-hole tank, and a drive unit. The rotating shaft drives the fixed ring and the single-hole tank to perform cleaning and solid-liquid separation. The single-hole tank, made of polystyrene or polyvinyl chloride, is combined with a motor drive to achieve closed-loop operation.
It simplifies the cleaning process during phage screening, reduces the risk of contamination during cleaning, and improves the automation and efficiency of the operation.
Smart Images
Figure CN223697038U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the technical field of phage single chain antibody panning, especially to a solid-liquid separation device for phage single chain antibody panning. BACKGROUND
[0002] In the field of antibody discovery such as biopharmaceuticals and medical research science, single chain antibodies are highly concerned due to their high diversity, easy availability and wide application. Phage screening is simple and efficient, and has become one of the most commonly used methods for screening single chain antibodies. In the current commonly used phage panning process, enzyme-linked immunosorbent method is usually used for panning, which requires multiple washing operations, and the operation is complicated and easy to be contaminated. Therefore, a closed semi-automatic solid-liquid separation device is needed to facilitate the removal of impurity phages and reduce contamination. SUMMARY
[0003] Therefore, the utility model aims at providing a solid-liquid separation device for phage single chain antibody panning.
[0004] In order to achieve the above purpose, the utility model adopts the technical scheme that:
[0005] A solid-liquid separation device for phage single chain antibody panning, comprising:
[0006] A housing having a containing space;
[0007] A rotating shaft, one end of which penetrates the housing and is rotatably connected with the housing;
[0008] A fixed ring, one end of the rotating shaft being fixedly connected with the fixed ring, and the axial direction of the fixed ring being perpendicular to the axial direction of the rotating shaft;
[0009] A single-hole groove, which is detachably connected with the fixed ring, and the fixed ring surrounds the outside of the single-hole groove;
[0010] A driving device for driving the rotating shaft to rotate.
[0011] The solid-liquid separation device for phage single chain antibody panning, wherein the housing comprises a box-shaped structure and a cover-shaped structure matched with the box-shaped structure;
[0012] The rotating shaft penetrates the cover-shaped structure and is rotatably connected with the cover-shaped structure.
[0013] The solid-liquid separation device for phage single chain antibody panning, wherein a bearing is arranged between the rotating shaft and the cover-shaped structure;
[0014] The inner ring of the bearing is fixedly connected with the rotating shaft, and the outer ring of the bearing is fixedly connected with the cover-shaped structure.
[0015] The rotating shaft is provided with a bearing fixing portion for fixing the bearing and limiting the position of the bearing in the axial direction of the rotating shaft.
[0016] The solid-liquid separation device for phage single-chain antibody panning has the flow outlet arranged at the bottom of the box-shaped structure.
[0017] The solid-liquid separation device for phage single-chain antibody panning has the sample adding hole arranged on the cover-shaped structure.
[0018] The solid-liquid separation device for phage single-chain antibody panning has one of the inner wall of the fixing ring and the outer wall of the single-hole groove provided with a ring-shaped protrusion, and the other of the inner wall of the fixing ring and the outer wall of the single-hole groove provided with a ring-shaped groove matched with the ring-shaped protrusion.
[0019] The fixing ring and the single-hole groove are in interference fit.
[0020] The solid-liquid separation device for phage single-chain antibody panning has both ends of the single-hole groove protruding from both ends of the fixing ring.
[0021] The solid-liquid separation device for phage single-chain antibody panning has the driving device being a charging motor.
[0022] The utility model discloses a solid-liquid separation device for phage single-chain antibody panning, which can satisfy the incubation and cleaning of target proteins and phages in the phage panning process, reduce the cumbersome cleaning operation and possible pollution in the cleaning process.
[0023] The utility model discloses a solid-liquid separation device for phage single-chain antibody panning, which can satisfy the incubation and cleaning of target proteins and phages in the phage panning process, reduce the cumbersome cleaning operation and possible pollution in the cleaning process. BRIEF DESCRIPTION OF DRAWINGS
[0024] In order to more clearly illustrate the technical scheme in the embodiments of the utility model, the following will briefly introduce the drawings needed to be used in the embodiment or the prior art description, and obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can also be obtained according to these drawings without paying the creative labor.
[0025] Figure 1 It is the first direction's stereogram schematic view of the solid-liquid separation device for phage single-chain antibody panning of the utility model.
[0026] Figure 2It is the second direction's stereogram of the solid-liquid separation device for phage single chain antibody panning of the utility model.
[0027] In the drawing: 1, drive device; 2, charging port; 3, rotating shaft; 4, sample adding hole; 5, cover structure; 6, fixed ring; 7, single-hole groove; 8, box structure; 9, flow outlet. DETAILED DESCRIPTION
[0028] In order to more clearly illustrate the technical scheme of the embodiments of the present specification, the embodiments will be described in detail below with reference to the drawings. Obviously, the following description is some examples or embodiments of the present specification, and for those skilled in the art, without creative labor, the technical scheme or means disclosed in the present specification can also be applied to other scenarios according to the technical content.
[0029] It should be understood that the "system", "device", "equipment", "part" and / or "unit" and / or "module" used in the present specification is a method for distinguishing different components, elements, parts, parts or assemblies at different levels. However, if other words can achieve the same purpose, the words can be replaced by other expressions.
[0030] In the present specification, the technical terms of components, elements, etc. are not specified by single number, but can also include plural. Generally speaking, the terms "include", "contain" and the like only indicate the inclusion of the steps, elements or components explicitly identified, and these steps, elements and components do not constitute an exclusive list, and the method or device described can also include other steps or components.
[0031] In the description of the present specification, it should be understood that the orientation description, such as the orientation or position relationship indicated by up, down, front, back, left, right and the like is based on the orientation or position relationship shown in the drawing, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, therefore it cannot be understood as a limitation on the present application. In the description of the present specification, unless otherwise explicitly limited, the words setting, installing, connecting and the like should be broadly understood, and those skilled in the art can reasonably determine the specific meaning of the above words in the present specification in combination with the specific content of the technical scheme.
[0032] Single-chain antibody panning can be a process of screening single-chain antibodies with specific binding properties from an antibody library using phage display technology. In some related embodiments, this process can include one or more of the following steps.
[0033] Construction of phage display antibody library: phage display antibody library containing a large number of different antibody genes. In some embodiments, the antibody genes can be derived from humans, rabbits, mice and other organisms, amplified and cloned into phage vectors by genetic engineering techniques, so that antibody fragments are fused and expressed with phage coat proteins and displayed on the surface of phage.
[0034] Antigen coating and incubation: specific antigens are coated on carriers such as single-well grooves, microwell plates or magnetic beads, and incubated with phage antibody library. In some embodiments, during incubation, antigens will selectively bind to antibodies that interact with them, forming antigen-antibody complexes.
[0035] Washing and screening: after incubation, unbound or non-specifically bound phage can be removed by washing steps to retain phage specifically bound to antigens. The washing and screening steps usually need to be carried out for multiple rounds to improve the percentage of positive phage.
[0036] Identification and analysis of positive phage: further identification and analysis of positive phage selected, for example, including detection of antibody affinity by enzyme-linked immunosorbent assay (ELISA), analysis of antibody gene sequence by DNA sequencing, and affinity ranking by competitive ELISA and other methods.
[0037] In some embodiments, by single-chain antibody screening technology, single-chain antibodies with specific binding properties can be quickly and efficiently screened from a large antibody library, providing support for the development of new vaccines and drugs. For example, in the field of tumor treatment, single-chain antibodies against specific tumor-related antigens can be screened for the preparation of targeted therapeutic drugs.
[0038] Figure 1 is the first direction of the solid-liquid separation device for phage single-chain antibody screening of the utility model, Figure 2 is the second direction of the solid-liquid separation device for phage single-chain antibody screening of the utility model. Referring to Figure 1 、 Figure 2 In one or more embodiments of the present specification, the solid-liquid separation device for phage single-chain antibody screening comprises a shell, a rotating shaft 3, a fixed ring 6, a single-well groove 7 and a driving device 1, wherein the shell has a containing space, the rotating shaft 3 is rotatably arranged, one end of the rotating shaft 3 penetrates the shell and is rotatably connected with the shell.
[0039] In some embodiments, one end of the rotating shaft 3 is fixedly connected with the fixed ring 6 to transmit rotating power to the fixed ring 6 to rotate the fixed ring 6. In some embodiments, the axial direction of the fixed ring 6 is arranged perpendicularly to the axial direction of the rotating shaft 3, the single-hole groove 7 is detachably connected with the fixed ring 6, the fixed ring 6 is arranged around the outside of the single-hole groove 7, and the single-hole groove 7 is rotated based on the rotation of the fixed ring 6. In some embodiments, the driving device 1 drives the rotating shaft 3 to rotate.
[0040] In some embodiments, the single-hole groove 7 is made of polystyrene or polyvinyl chloride material and can be combined with protein.
[0041] In one or more embodiments of the present specification, the shell comprises a box-shaped structure 8 and a cover-shaped structure 5 matched with the box-shaped structure 8. In some embodiments, the box-shaped structure 8 and the cover-shaped structure 5 are detachably connected.
[0042] In some embodiments, the rotating shaft 3 penetrates the cover-shaped structure 5 and is rotatably connected with the cover-shaped structure 5. In some embodiments, as shown in FIG. 1, the upper end and the lower end of the rotating shaft 3 protrude out of the cover-shaped structure 5, and the middle part of the rotating shaft is limited in the cover-shaped structure 5 and can rotate relative to the cover-shaped structure 5 to facilitate the transmission of rotating power to the fixed ring 6 and the single-hole groove 7 located inside the shell. Figure 1 Figure 2 In some embodiments, the rotating shaft 3 penetrates the cover-shaped structure 5 and is rotatably connected with the cover-shaped structure 5. In some embodiments, as shown in FIG. 1, the upper end and the lower end of the rotating shaft 3 protrude out of the cover-shaped structure 5, and the middle part of the rotating shaft is limited in the cover-shaped structure 5 and can rotate relative to the cover-shaped structure 5 to facilitate the transmission of rotating power to the fixed ring 6 and the single-hole groove 7 located inside the shell.
[0043] In some embodiments, a bearing is arranged between the rotating shaft 3 and the cover-shaped structure 5, the inner ring of the bearing is fixedly connected with the rotating shaft 3, the outer ring of the bearing is fixedly connected with the cover-shaped structure 5, and the rotating shaft 3 has a bearing fixing part for fixing the bearing and limiting the position of the bearing in the axial direction of the rotating shaft.
[0044] In some embodiments, the cover-shaped structure 5 is provided with a hole for the rotating shaft 3 to penetrate. In some embodiments, the bearing is arranged between the inner wall of the hole of the cover-shaped structure 5 and the outer wall of the bearing fixing part of the rotating shaft 3. In some embodiments, the inner wall of the hole of the cover-shaped structure 5 is provided with two bearing retainer groove slots, and each of the two bearing retainer groove slots is provided with a bearing retainer. In some embodiments, the outer ring of the bearing can be limited between the two bearing retainers.
[0045] In some embodiments, the outer wall of the rotating shaft 3 can include a stepped part forming the bearing fixing part. In some embodiments, one end of the inner ring of the bearing can abut against the stepped part, and the other end of the inner ring of the bearing can be fastened by a fastener such as a nut.
[0046] In some embodiments, the bottom of the box-shaped structure 8 is provided with a flow outlet 9. In some embodiments, the flow outlet 9 is correspondingly provided with a flow outlet cover.
[0047] In some embodiments, the cover-like structure 5 is provided with a sample adding hole 4. In some embodiments, the sample adding hole 4 is correspondingly provided with a sample adding hole cover.
[0048] In one or more embodiments of the present specification, one of the inner wall of the fixing ring 6 and the outer wall of the single-hole groove 7 is provided with a ring-shaped protrusion, and the other of the inner wall of the fixing ring 6 and the outer wall of the single-hole groove 7 is provided with a ring-shaped groove matched with the ring-shaped protrusion. In some embodiments, the fixing ring 6 and the single-hole groove 7 are in interference fit to achieve detachable fixing of the single-hole groove 7 relative to the fixing ring 6.
[0049] In other embodiments, the single-hole groove 7 can also be fixed to the fixing ring 6 by welding or the like.
[0050] In one or more embodiments of the present specification, both ends of the single-hole groove 7 protrude from both ends of the fixing ring 6.
[0051] In one or more embodiments of the present specification, the driving device 1 is a charging motor, and the driving device 1 is provided with a charging port 2.
[0052] In one or more embodiments of the present specification, for the solid-liquid separation device for phage screening of monoclonal antibodies described above, an exemplary use method is introduced, and in actual use, it can be reasonably adjusted according to the actual situation, rather than necessarily operating in the same way.
[0053] In some embodiments, in use, the target protein is incubated with the single-hole groove overnight to coat the protein, the buffer washing liquid is added in the shell, the driving device 1 such as a motor is started, and other liquids in the single-hole groove 7 are washed by rotating, after washing is completed, the buffer washing liquid is discharged from the flow outlet 9, and the driving device 1 such as a motor is started again, which can shake out the residual liquid in the single-hole groove 7, so as to achieve the washing and solid-liquid separation effect in the single-chain antibody screening.
[0054] The above only describes the preferred embodiments of the present application, and does not limit the implementation and protection scope of the present application. For those skilled in the art, it should be realized that any equivalent replacement and obvious changes made by applying the contents of the present application specification and drawings should be included in the protection scope of the present application.
Claims
1. A solid-liquid separation device for panning bacteriophage single-chain antibodies, characterized in that, include: A housing having an accommodating space; A rotating shaft, one end of which passes through the housing and is rotatably connected to the housing; A fixed ring is provided, one end of which is fixedly connected to the rotating shaft, and the axial direction of the fixed ring is perpendicular to the axial direction of the rotating shaft. A single-hole groove, wherein the single-hole groove is detachably connected to the retaining ring, and the retaining ring surrounds the outside of the single-hole groove; A driving device that drives the rotating shaft to rotate.
2. The solid-liquid separation device for screening bacteriophage single-chain antibodies according to claim 1, characterized in that, The housing includes a box-shaped structure and a cover-shaped structure that matches the box-shaped structure; The rotating shaft passes through the cover-like structure and is rotatably connected to the cover-like structure.
3. The solid-liquid separation device for screening bacteriophage single-chain antibodies according to claim 2, characterized in that, A bearing is provided between the rotating shaft and the cover-like structure; The inner ring of the bearing is fixedly connected to the rotating shaft, and the outer ring of the bearing is fixedly connected to the cover-like structure. The rotating shaft has a bearing fixing part for fixing the bearing and limiting the position of the bearing in the axial direction of the rotating shaft.
4. The solid-liquid separation device for screening bacteriophage single-chain antibodies according to claim 2, characterized in that, The bottom of the box-shaped structure is provided with an outlet.
5. The solid-liquid separation device for screening bacteriophage single-chain antibodies according to claim 2, characterized in that, The cap-like structure is provided with a sample application hole.
6. The solid-liquid separation device for panning bacteriophage single-chain antibodies according to claim 1, characterized in that, One of the inner wall of the fixing ring and the outer wall of the single-hole groove is provided with an annular protrusion, and the other of the inner wall of the fixing ring and the outer wall of the single-hole groove is provided with an annular groove that matches the annular protrusion. The retaining ring and the single-hole groove are interference-fitted.
7. The solid-liquid separation device for screening bacteriophage single-chain antibodies according to claim 1, characterized in that, Both ends of the single-hole groove protrude beyond the two ends of the fixing ring.
8. The solid-liquid separation device for screening bacteriophage single-chain antibodies according to claim 1, characterized in that, The driving device is a rechargeable motor.