Automatic protein concentration and purification system

Abstract

The utility model discloses an automatic protein concentration and purification system which comprises a liquid storage tank, an ultrafilter, a liquid inlet pump, a liquid outlet pump and a controller, the left side and the right side of the liquid storage tank are respectively provided with a filtrate inlet and a concentrated liquid inlet, and the filtrate inlet and the concentrated liquid inlet are respectively connected with a permeation pipe and a liquid return pipe of the ultrafilter. The bottom of the liquid storage tank is connected with a stock solution inlet pipe, a product liquid outlet pipe and a stock solution outlet pipe, the stock solution outlet pipe is connected with a liquid outlet pump, the stock solution inlet pipe is connected with a liquid inlet pump, the permeation pipe and the liquid return pipe are respectively connected with a filtrate discharge pipe and a concentrated liquid discharge pipe, and the permeation pipe and the liquid return pipe are provided with flowmeters. Pressure sensors are arranged on the permeation pipe, the liquid return pipe and the stock solution outlet pipe, temperature sensors are arranged in the liquid storage tank and on the stock solution outlet pipe, automatic switch valves are arranged on the pipelines, and the automatic switch valves and the various sensors are in signal connection with a controller. The device can be used for circularly concentrating and purifying protein, and is high in automation degree, simple to operate and high in efficiency.

Description

Technical Field

[0001] This utility model relates to the field of raw material purification technology, specifically to an automated protein concentration and purification system. Background Technology

[0002] Protein concentration technology belongs to the field of biological macromolecule concentration technology. Its purpose is to remove water, ions, and other small molecules from protein solutions using physical or chemical methods, thereby significantly increasing the protein concentration per unit volume. Current production methods use storage tanks to store protein products and ultrafiltration to purify the protein. However, the storage tanks and ultrafiltration systems are controlled by different systems. During production, operators need to control both the storage tanks and the ultrafiltration system. Furthermore, before each production run, cleaning equipment must be manually connected, and after cleaning, the equipment must be replaced with the production equipment. This process is cumbersome, labor-intensive, and inefficient. Utility Model Content

[0003] This invention provides an automated protein concentration and purification system that integrates a storage tank and an ultrafilter into the same control system. It can circulate and concentrate and purify proteins, has a high degree of automation in the production process, and integrates the production pipeline and the cleaning pipeline, simplifying operation and increasing efficiency.

[0004] To achieve the above-mentioned technical objectives, the technical solution adopted by this utility model is as follows:

[0005] An automated protein concentration and purification system includes a storage tank, an ultrafilter, an inlet pump, an outlet pump, and a controller. The storage tank has a filtrate inlet and a concentrate inlet on its left and right sides, respectively. The filtrate inlet and concentrate inlet are connected to the permeation pipe and return pipe of the ultrafilter, respectively. The bottom of the storage tank is connected to a raw liquid inlet pipe, a product outlet pipe, and a raw liquid outlet pipe, respectively. An outlet pump is connected to the raw liquid outlet pipe, and an inlet pump is connected to the raw liquid inlet pipe. The permeation pipe and return pipe are connected to the filtrate drain pipe and the concentrate drain pipe, respectively. Flow meters are installed on the permeation pipe and return pipe, and pressure sensors are installed on the permeation pipe, return pipe, and raw liquid outlet pipe. Temperature sensors are installed inside the storage tank and on the raw liquid outlet pipe. Automatic on / off valves are installed on each pipe. The inlet pump, outlet pump, automatic on / off valves, flow meters, pressure sensors, and temperature sensors are all signal-connected to the controller.

[0006] Furthermore, the ultrafilter is equipped with an ultrafiltration membrane inside, which divides the inner cavity of the ultrafilter into a filtrate chamber and a concentrate chamber. A permeation tube connects to the filtrate chamber, and a raw liquid outlet tube connects to the concentrate chamber.

[0007] Furthermore, the filtrate chamber and the concentrate chamber are respectively connected to conductivity sensors.

[0008] Furthermore, a liquid filling pipe is connected to the top of the liquid storage tank, and a spray head is connected to the lower end of the liquid filling pipe.

[0009] Furthermore, a stirrer is provided at the bottom of the storage tank.

[0010] Furthermore, a sight glass is provided on the top of the liquid storage tank.

[0011] Furthermore, both the raw liquid inlet pipe and the return liquid pipe are connected to compressed air inlet pipes.

[0012] Furthermore, the outer wall of the storage tank is provided with a temperature control jacket.

[0013] Furthermore, a weighing module is provided at the bottom of the liquid storage tank.

[0014] Furthermore, there are multiple ultrafilters, which are integrated together in series and parallel.

[0015] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0016] The storage tank and ultrafilter of this invention are connected together through a circulation pipeline and are controlled by the same control system. By repeatedly circulating and concentrating the protein stock solution, a high concentration of protein is obtained. The production process is highly automated. The production pipeline and cleaning pipeline are integrated, which simplifies the production and cleaning operations and improves efficiency. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a simplified structural diagram of the present invention;

[0019] Figure 2 This is the system control schematic diagram;

[0020] Figure 3 This is an engineering schematic diagram of the industrial application of this utility model;

[0021] In the diagram: 1-Storage tank, 2-Ultrafilter, 3-Inlet pump, 4-Outlet pump, 5-Permeate tube, 6-Return tube, 7-Supplier inlet tube, 8-Product outlet tube, 9-Supplier outlet tube, 10-Filtrate drain tube, 11-Concentrate drain tube, 12-Ultrafiltration membrane, 13-Filtrate chamber, 14-Concentrate chamber, 15-Conductivity sensor, 16-Addition tube, 17-Spray head, 18-Sight glass, 19-Temperature control jacket, 20-Weighing module. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. The components of the embodiments of this application described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0023] In the description of the embodiments of this application, it should be noted that the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship that the product of this application is usually placed in when in use, or the orientation or positional relationship that is commonly understood by those skilled in the art. It is only for the convenience of describing this application and simplifying the description, and is not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of this application.

[0024] In the description of the embodiments of this application, it should also be noted that, unless otherwise expressly specified and limited, the terms "set," "install," and "connect" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0025] like Figure 1As shown, an automated protein concentration and purification system includes a storage tank 1, an ultrafilter 2, an inlet pump 3, an outlet pump 4, and a controller. The storage tank 1 has a filtrate inlet and a concentrate inlet on its left and right sides, respectively. The filtrate inlet and concentrate inlet are connected to the permeation pipe 5 and return pipe 6 of the ultrafilter 2, respectively. The ultrafilter 2 has an ultrafiltration membrane 12 inside, which divides the inner cavity of the ultrafilter 2 into a filtrate chamber 13 and a concentrate chamber 14. The permeation pipe 5 connects to the filtrate chamber 13, and the concentrate outlet pipe 9 connects to the concentrate chamber 14. The filtrate chamber 13 and the concentrate chamber 14 are respectively connected to conductivity sensors 15 for monitoring water quality. The bottom of the storage tank 1 is connected to the raw liquid inlet pipe 7, the product outlet pipe 8, and the raw liquid outlet pipe 9, respectively. An outlet pump 4 is connected to the raw liquid outlet pipe 9, and an inlet pump 3 is connected to the raw liquid inlet pipe 7. The permeate pipe 5 and the return pipe 6 are connected to the filtrate outlet pipe 10 and the concentrate outlet pipe 11, respectively. During system operation, the protein raw liquid is first pumped into the storage tank 1 by the inlet pump 3, and then introduced into the ultrafilter by the outlet pump 4. After filtration by the ultrafilter 2, the concentrated macromolecular liquid (containing protein) flows back to the storage tank 1 through the return pipe 6. The filtered small molecule liquid is discharged through the permeate pipe 5 and the filtrate outlet pipe 10. The storage tank 1 and the ultrafilter 2 form a circulation path, allowing the returned protein liquid to be circulated and filtered multiple times to improve protein purity, while the small molecule liquid can be recovered. Besides protein purification, this system can also be used for the separation of other liquids. It can also selectively circulate and purify small molecule liquids as needed, and the separated macromolecular liquid is discharged from the concentrate outlet pipe 11.

[0026] Flow meters and ultraviolet sensors are installed on through pipe 5 and return pipe 6. Pressure sensors are installed on through pipe 5, return pipe 6, and raw liquid outlet pipe 9. Temperature sensors are installed in storage tank 1 and on raw liquid outlet pipe 9. Automatic switching valves are installed on each pipe. Inlet pump 3, outlet pump 4, automatic switching valves, flow meters, pressure sensors, temperature sensors, and ultraviolet sensors are all connected to the controller. The controller has a built-in CPU and can control the operation of the entire system according to the received signals to achieve automated management. The control principle is as follows. Figure 2 As shown, the liquid transfer pump includes an inlet pump 3 and an outlet pump 4. Preferably, there are multiple ultrafilters 2, integrated together through series and parallel connections, such as... Figure 3 As shown, the valves on the through pipe 5 and the return pipe 6 and their branches are automatic regulating valves, which can adjust the pipeline flow. The controller is electrically connected to a host computer, which has a user interface that allows operators to view the system status and has buttons to operate the equipment, adjust parameters, and collect data during system operation, realizing industrial information visualization.

[0027] Preferably, the top of the storage tank 1 is connected to a liquid filling pipe 16, and the lower end of the liquid filling pipe 16 is connected to a spray head 17 for spraying buffer solution to adjust the pH of the storage tank 1. It can also spray cleaning solution to clean the storage tank 1. The cleaning solution can clean the entire system through a circulation pipeline, and the waste liquid after cleaning is discharged from the drain pipe.

[0028] Preferably, the bottom of the storage tank 1 is equipped with a stirrer for stirring the original liquid.

[0029] Preferably, the top of the liquid storage tank 1 is provided with a sight glass 18, which can be used to observe the condition inside the tank.

[0030] Preferably, both the raw liquid inlet pipe and the return liquid pipe are connected to compressed air inlet pipes, so that compressed air can be introduced to purge and drain the residual liquid when cleaning the entire system.

[0031] Preferably, the outer wall of the liquid storage tank 1 is provided with a temperature control jacket 19, which can be heated by a built-in electric heating wire or an electric hot water pipe, and the heating temperature can be adjusted according to the needs of the production process.

[0032] Preferably, the bottom of the liquid storage tank 1 is provided with a weighing module 20 for real-time monitoring of the mass change of the liquid storage tank 1.

[0033] Of course, there may be other embodiments of this utility model. Without departing from the spirit and essence of this utility model, those skilled in the art can make various corresponding changes and modifications based on this utility model, but these corresponding changes and modifications should all fall within the protection scope of the appended claims of this utility model.

Claims

1. An automated protein concentration purification system, characterized by: The application relates to a liquid storage tank (1), an ultrafilter (2), a liquid inlet pump (3), a liquid outlet pump (4) and a controller, wherein the liquid storage tank (1) is provided with a filtered liquid inlet on the left side and a concentrated liquid inlet on the right side; the filtered liquid inlet and the concentrated liquid inlet are connected with a permeation pipe (5) and a return pipe (6) of the ultrafilter (2) respectively; the bottom of the liquid storage tank (1) is connected with a raw liquid inlet pipe (7), a product outlet pipe (8) and a raw liquid outlet pipe (9); the raw liquid outlet pipe (9) is connected with the liquid outlet pump (4); the raw liquid inlet pipe (7) is connected with the liquid inlet pump (3); the permeation pipe (5) and the return pipe (6) are connected with a filtered liquid outlet pipe (10) and a concentrated liquid outlet pipe (11) respectively; the permeation pipe (5) and the return pipe (6) are provided with flow meters; the permeation pipe (5), the return pipe (6) and the raw liquid outlet pipe (9) are provided with pressure sensors; the liquid storage tank (1) and the raw liquid outlet pipe (9) are provided with temperature sensors; automatic on-off valves are arranged on the pipes; the liquid inlet pump (3), the liquid outlet pump (4), the automatic on-off valves, the flow meters, the pressure sensors and the temperature sensors are signal-connected with the controller.

2. The automated protein concentration purification system of claim 1, wherein: The ultrafilter (2) is internally provided with an ultrafiltration membrane (12), which divides the inner cavity of the ultrafilter (2) into a filtered liquid cavity (13) and a concentrated liquid cavity (14); the permeation pipe (5) is connected with the filtered liquid cavity (13); and the raw liquid outlet pipe (9) is connected with the concentrated liquid cavity (14).

3. The automated protein concentration purification system of claim 2, wherein: The filtered liquid cavity (13) and the concentrated liquid cavity (14) are connected with electric conductivity sensors (15) respectively.

4. The automated protein concentration purification system of claim 1, wherein: The top of the liquid storage tank (1) is connected with a liquid adding pipe (16), and the lower end of the liquid adding pipe (16) is connected with a spraying head (17).

5. The automated protein concentration purification system of claim 1, wherein: The bottom of the liquid storage tank (1) is provided with a stirrer.

6. The automated protein concentration purification system of claim 1, wherein: The top of the liquid storage tank (1) is provided with a sight glass (18).

7. The automated protein concentration purification system of claim 1, wherein: The raw liquid inlet pipe and the return pipe are both connected with compressed air inlet pipes.

8. The automated protein concentration purification system of claim 1, wherein: The outer wall of the liquid storage tank (1) is provided with a temperature control jacket (19).

9. The automated protein concentration purification system of claim 1, wherein: The bottom of the liquid storage tank (1) is provided with a weighing module (20).

10. The automated protein concentration purification system of claim 1, wherein: The ultrafilter (2) is multiple and is integrated by being connected in series and in parallel.

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