Multiplex fluorescent PCR (Polymerase Chain Reaction) detection kit

Abstract

The utility model discloses a multiplex fluorescence PCR (Polymerase Chain Reaction) detection kit, which comprises a kit body and a kit cover, a placing table is arranged in the kit body, a reagent bottle fixing seat is arranged on the placing table, and a closed cavity for placing dry ice is formed between the reagent bottle fixing seat and the lower end in the kit body. Compared with the prior art, the swine fever dual-fluorescence PCR detection kit has the beneficial effects that in the prior art, after dry ice is placed in the swine fever dual-fluorescence PCR detection kit, the reagent bottle fixing seat and the heat conduction pipe on the reagent bottle fixing seat are difficult to accurately and conveniently place; the use efficiency of the kit is influenced. According to the kit, dry ice is separated by designing the isolation tube, so that the heat conduction tube can be easily and accurately inserted from the upper end of the isolation tube in a sliding manner, the blocking of the dry ice is avoided, and the use convenience and efficiency of the kit are improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to detection kit technical field, concretely is a multiple fluorescence PCR detection kit. BACKGROUND

[0002] In the biological experiment and detection field, the fluorescence PCR detection kit is widely applied because of its high efficiency, sensitivity and specificity. Especially, for the detection of animal epidemic diseases such as swine fever, double or multiple fluorescence PCR detection kits are more popular because they can detect multiple pathogens at the same time.

[0003] However, the existing swine fever double fluorescence PCR detection kit, such as the kit disclosed in the Chinese utility model patent with application number 202021622922.3, although it is designed with dry ice cavity and reagent bottle fixing seat to maintain the low temperature environment during the detection process, there are still some problems in actual operation. Specifically, when the dry ice is put into the dry ice cavity, it is difficult to place the reagent bottle fixing seat and the heat conducting pipe on it due to the blocking effect of the dry ice, which affects the convenience and efficiency of the kit. SUMMARY

[0004] The utility model aims at the deficiencies of the prior art, and provides a multiple fluorescence PCR detection kit to solve the problems in the background art.

[0005] The utility model provides the following technical scheme: a multiple fluorescence PCR detection kit, comprising a box body and a box cover, the inside of the box body is provided with a resting table, the resting table is provided with a reagent bottle fixing seat, a closed cavity for placing dry ice is formed between the reagent bottle fixing seat and the lower end of the inside of the box body, and the reagent bottle fixing seat is provided with a reagent bottle fixing hole, the reagent bottle fixing hole is fixed with a heat conducting pipe, the inside of the heat conducting pipe is used for placing a reagent bottle, the lower end of the inside of the box body is fixed with a separation tube for separating dry ice, the upper end of the separation tube is open, and the lower end of the heat conducting pipe is slidably inserted into the upper end of the separation tube.

[0006] Compared with the prior art, the utility model has the advantages that in the prior art, after placing dry ice, the reagent bottle fixing seat and the heat conducting pipe thereon are difficult to be accurately and conveniently placed, which affects the use efficiency of the kit. The utility model separates the dry ice by designing a separation tube, so that the heat conducting pipe can be easily and accurately slid into the upper end of the separation tube, avoiding the blocking of the dry ice, and improving the use convenience and efficiency of the kit. BRIEF DESCRIPTION OF DRAWINGS

[0007] Fig. 1 It is a front view of the utility model.

[0008] Fig. 2 It is the structure schematic view when the reagent bottle fixing seat is not installed.

[0009] Fig. 3 It is the explosion structure schematic view. DETAILED DESCRIPTION

[0010] The technical solutions in the embodiments of the present application will be clearly and completely described with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative work fall within the scope of the present application.

[0011] Please refer to Figs. 1-3 The utility model provides a multiple fluorescence PCR detection reagent box, including box 1 and box cover 2, the inside of box 1 is equipped with the rest platform 3, is provided with reagent bottle fixing seat 4 on the rest platform 3, and the inside lower end between reagent bottle fixing seat 4 and box 1 forms the closed cavity 6 for placing dry ice 5, and reagent bottle fixing seat 4 is opened with reagent bottle fixing hole, and reagent bottle fixing hole is fixed with heat pipe 7, and heat pipe 7 is used for placing reagent bottle inside, uses, first opens box cover 2, takes out reagent bottle fixing seat 4, puts dry ice 5 into closed cavity 6, then places reagent bottle fixing seat 4 on the rest platform 3, makes heat pipe 7 insert into closed cavity 6, then reagent bottle is inserted into heat pipe 7 and closes box cover 2, the above is prior art, and here is not repeated.

[0012] On the basis of prior art, the utility model still has the following innovative design:

[0013] The lower end of the inside of box 1 is fixed with the isolation pipe 8 for separating dry ice 5, the thickness of isolation pipe 8 is between 1-2mm, and the upper end of isolation pipe 8 is opened, when using, the lower end of heat pipe 7 can be slid from the upper end of isolation pipe 8 and inserted, through the setting of isolation pipe 8, when placing dry ice 5, dry ice 5 can be placed in the gap between isolation pipe 8 first, then gently shakes box 1, makes dry ice 5 as evenly as possible, when installing reagent bottle fixing seat 4, the lower end of heat pipe 7 is inserted into isolation pipe 8, in this way, it can prevent dry ice 5 from causing obstruction to the insertion of heat pipe 7.

[0014] It can be understood that after heat pipe 7 is inserted into isolation pipe 8, isolation pipe 8 and heat pipe 7 constitute a double-layer structure, which has sufficient strength, in order to ensure the heat conduction effect, heat pipe 7 can be designed to be thinner.

[0015] The inner wall of box 1 is embedded with cavity 9, and the setting of cavity 9 can increase the heat preservation effect of box 1.

[0016] In some embodiments, the surface of the rest table 3 is provided with a sticking layer 10 for sticking the reagent bottle fixing seat 4, which can position the reagent bottle fixing seat 4 on one side and play a sealing role between the reagent bottle fixing seat 4 and the rest table 3 on the other side.

[0017] In some embodiments, the side surface of the box body 1 is provided with a one-way exhaust valve 11 for pressure relief.

[0018] In some embodiments, in order to better cool the bottom of the reagent bottle, the bottom side surface of the isolation tube 8 is provided with a through hole 12, so that the gasified dry ice can enter the inside of the isolation tube 8 through the through hole 12 to better cool the bottom of the heat conduction pipe 7.

[0019] In summary, the utility model aims at realizing a multiple fluorescent PCR detection reagent box, which separates the dry ice 5 by designing the isolation tube 8, so that the heat conduction pipe 7 can be easily and accurately inserted into the area where the dry ice 5 is located when the reagent bottle fixing seat 4 is installed, avoiding the blockage of the dry ice 5 to the insertion of the heat conduction pipe 7, and improving the use convenience and efficiency of the reagent box.

[0020] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirits of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A multiplex fluorescent PCR detection kit, comprising a box body and a box cover, an inner part of the box body is provided with a resting table, a reagent bottle fixing seat is arranged on the resting table, a closed cavity for placing dry ice is formed between the reagent bottle fixing seat and the lower end of the inner part of the box body, and the reagent bottle fixing seat is provided with a reagent bottle fixing hole, a heat conducting pipe is fixed in the reagent bottle fixing hole, and the heat conducting pipe is used for placing a reagent bottle, characterized in that: a separation tube for separating dry ice is fixed to the lower end of the inner part of the box body, the upper end of the separation tube is open, and the lower end of the heat conducting pipe is slidably inserted into the upper end of the separation tube. A cavity is embedded in the inner wall of the box body.

2. The multiplex fluorescent PCR detection kit according to claim 1, characterized in that: The surface of the resting table is provided with a sticking layer for sticking the reagent bottle fixing seat.

3. The multiplex fluorescent PCR detection kit according to claim 1, characterized in that: The side surface of the box body is provided with a one-way exhaust valve.

4. The multiplex fluorescent PCR detection kit according to claim 1, characterized in that: The thickness of the separation tube is between 1-2mm.

5. The multiplex fluorescent PCR detection kit according to claim 1, characterized in that: A through hole is formed through the bottom side of the separation tube.

6. The multiplex fluorescent PCR detection kit according to claim 5, characterized in that: ​

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