Clamping assembly for microorganism separation and inoculation
By combining a clamping component with a 3D camera, the problem of precise probe distance control in existing clamping devices has been solved, enabling precise gripping of petri dishes and accurate picking of colonies, thus improving the quality and efficiency of microbial isolation.
Patent Information
- Application Number
- CN202423303869.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-27
- Publication Date
- 2025-12-23
- Estimated Expiration
- 2034-12-27
AI Technical Summary
In microbial isolation operations, existing clamping devices have difficulty in accurately controlling the probe distance, leading to problems such as petri dish breakage or missed colonies.
A clamping assembly was designed, comprising a fixed clamping plate, a cylinder mounting plate, a cylinder slider plate, a clamping movable plate, a clamping fixed plate, an inoculation needle, and a three-jaw cylinder. It is combined with a 3D camera for colony positioning and precise picking, and uses the cylinder and jaws to grasp and move the culture dish.
It enables precise clamping of petri dishes and accurate picking of colonies, improving the quality and efficiency of microbial isolation.
Smart Images
Figure CN223705580U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to the field of microorganism separation inoculation device, concretely relates to a microorganism separation inoculation clamping assembly. BACKGROUND
[0002] In the field of clinical microorganism inspection, microorganism related teaching and scientific research experiment, etc. involving microorganism separation, since the sample to be detected is a mixture of various different bacteria or different subtype microorganisms, if the sample is to be accurately identified or subsequent experiment, the relatively pure microorganism colony needs to be obtained, therefore, the microorganism separation is particularly important.
[0003] In the operation of microorganism separation, since the volume of the culture medium injected in the culture dish is different, the distance between the colony and the probe is different: if the probe is lowered too deep, the needle may directly touch the bottom of the culture dish and cause the culture dish to break or the needle to bend; but if the probe is lowered too shallow, the colony may be missed because the probe cannot touch the surface of the colony. The above operations all need to use a clamping device, and the quality of the microorganism separation is directly affected by the operation of the clamping device. SUMMARY
[0004] In order to solve the problems in the prior art, the inventors have developed a microorganism separation inoculation clamping assembly based on their rich experience in the field of microorganism separation inoculation device.
[0005] In order to achieve the above technical purposes, the utility model adopts the following technical scheme: a microorganism separation inoculation clamping assembly, comprising a fixed clamping plate, a gas cylinder mounting plate, a gas cylinder sliding block plate, a clamping movable plate, a clamping fixed plate, an inoculation needle and a culture dish, the inoculation needle is fixed on the fixed clamping plate, the inoculation needle is fixed on the clamping movable plate by screws, the clamping movable plate is connected with the gas cylinder sliding block plate by screws, the gas cylinder sliding block plate is connected with the gas cylinder mounting plate by screws, the gas cylinder mounting plate is welded on one side of the fixed clamping plate, a camera fixing plate is further connected to the fixed clamping plate, a three-jaw gas cylinder is built-in the fixed clamping plate, and the culture dish is placed below the three-jaw gas cylinder.
[0006] Further, the three-jaw gas cylinder is provided with three clamping jaws, the included angle between two adjacent clamping jaws is 60°, and the culture dish is fixed on the three-jaw gas cylinder by the clamping jaws.
[0007] Further, a camera is installed on the camera fixing plate, and the camera positions the colony in the culture dish.
[0008] Further, after the camera positions the colony in 3D, the cylinder slide plate moves downward, at which time the inoculation needle dips the target colony positioned in the culture dish; then the cylinder slide moves upward, the three-jaw cylinder releases the clamping jaw, moves to above the culture dish, the clamping jaw grabs the culture dish downward, and then lifts to move the culture dish to the storage position; the clamping jaw grabs the clean culture dish from the incoming material to the streaking position, the three-jaw cylinder is released, the camera positions the colony in 3D again, and the above steps are repeated, so that the inoculation operation can be continuously completed.
[0009] Further, the inoculation needle is arranged at an inclined angle, the end of the inoculation needle close to the culture dish is low in position, and the end of the inoculation needle away from the culture dish is high in position.
[0010] Compared with the prior art, the utility model has the following beneficial effects:
[0011] The utility model discloses a fixed clamping plate, cylinder mounting plate, cylinder slide plate, clamping movable plate, clamping fixed plate, inoculation needle and other devices are arranged, the culture dish can be accurately grabbed in the process of accurately picking the target colony. BRIEF DESCRIPTION OF DRAWINGS
[0012] Fig. 1 It is the structure schematic view of the utility model.
[0013] Fig. 2 It is the side view of the utility model.
[0014] Reference signs: 1, cylinder mounting plate, 2, cylinder slide plate, 3, clamping movable plate, 4, clamping fixed plate, 5, inoculation needle, 6, clamping jaw, 7, culture dish, 8, camera fixed plate, 9, three-jaw cylinder, 10, fixed clamping plate. DETAILED DESCRIPTION
[0015] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.
[0016] As Figs. 1-2As shown, a microbial separation inoculation clamping assembly includes a fixed clamping plate 10, a cylinder mounting plate 1, a cylinder slider plate 2, a clamping movable plate 3, a clamping fixed plate 4, an inoculation needle 5 and a culture dish 7, the inoculation needle 5 is fixed on the fixed clamping plate 10, the inoculation needle 5 is fixed on the clamping movable plate 3 by a screw, the clamping movable plate 3 is connected with the cylinder slider plate 2 by a screw, the cylinder slider plate 2 is connected with the cylinder mounting plate 1 by a screw, the cylinder mounting plate 1 is welded on one side of the fixed clamping plate 10, the fixed clamping plate 10 is also connected with a camera fixing plate 8, the fixed clamping plate 10 is internally provided with a three-jaw cylinder 9, and the culture dish 7 is placed below the three-jaw cylinder 9.
[0017] In the embodiment, the three-jaw cylinder 9 is provided with three clamping jaws 6, adjacent two clamping jaws 6 are 60° apart, and the culture dish 7 is fixed on the three-jaw cylinder 9 by the clamping jaws 6. The inventor designs the clamping jaws 6 to be 60° apart, which is beneficial to the clamping jaws 6 to grab the culture dish 7.
[0018] The three-jaw cylinder 9 controls the clamping jaws 6 to clamp and release, and the air source of the three-jaw cylinder 9 comes from a compressed air tank, the air source pressure in the compressed air tank is not less than 0.5 Mpa, and in daily work, the operator often checks whether the pressure of the compressed air tank meets the working requirements.
[0019] In the embodiment, a camera is installed on the camera fixing plate 8, and the camera positions the colonies in the culture dish 7.
[0020] In the embodiment, after the camera positions the colonies in 3D, the cylinder slider plate 2 moves downward, at this time the inoculation needle 5 dips the target colonies positioned in the culture dish 7; then the cylinder slider 2 moves upward, the three-jaw cylinder 9 releases the clamping jaws 6, moves to above the culture dish 7, the clamping jaws 6 grab the culture dish 7 downward, then lifts up, and moves the culture dish 7 to a storage position; the clamping jaws 6 grab the clean culture dish 7 from the incoming material to the scribing position, the three-jaw cylinder 9 is released, the camera positions the colonies in 3D again, and the above steps are repeated, so that the inoculation work can be continuously completed.
[0021] In the embodiment, the inoculation needle 5 is arranged at an inclined angle, one end of the inoculation needle 5 close to the culture dish 7 is low in position, and one end of the inoculation needle 5 away from the culture dish 7 is high in position. The angle between the inoculation needle 5 and the horizontal plane is 30°-60°.
[0022] In practical use, this invention employs a mechanical positioning method to blindly grasp the culture dish 7, placing it within the field of view of a 3D camera. After opening the lid, a 2D camera is used to capture images of the culture dish 7 for colony localization, and a set of centroid coordinates of the target colony is selected based on a machine learning model. The 3D camera scans the culture dish 7, and the height information of the target colony's centroid is determined based on the positional calibration relationship between the 3D and 2D cameras. The target point of the robotic arm is determined based on the calibration relationship between the 2D camera and the inoculation needle, guiding the robotic arm to pick up the target colony. The robotic arm removes the previously photographed culture dish 7, takes an uninoculated clean culture dish 7, places it within the field of view of the 3D camera, scans it, obtains the height information of the upper surface of the culture medium, and guides the robot to perform streaking on the plate.
[0023] This clamping component can perform actions such as gripping the culture dish 7, clamping the inoculation needle 5, picking up colonies, and streak plating during microbial isolation and inoculation.
[0024] The colony picking process utilizes a clamping component designed to accommodate varying colony heights and different surface heights on the culture medium in petri dishes 7. Through precise design, the clamping mechanism adapts to different petri dish sizes, and the inoculation loop is held at various angles and positions, playing a crucial role in the entire microbial isolation and inoculation process.
[0025] The above embodiments are merely illustrative of the technical concept and features of this utility model, and are intended to enable those skilled in the art to understand the content of this utility model and implement it accordingly. They should not be construed as limiting the scope of protection of this utility model. All equivalent changes or modifications made based on the substance of the content of this utility model should be covered within the scope of protection of this utility model.
[0026] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.
Claims
1. A clamping assembly for microbial isolation and inoculation, characterized in that: The utility model provides a kind of inoculation device, including fixed clamping plate, cylinder mounting plate, cylinder slider plate, clamping movable plate, clamping fixed plate, inoculation needle and culture dish, inoculation needle is fixed on the fixed clamping plate, inoculation needle is fixed on clamping movable plate by screw, clamping movable plate is connected by screw with cylinder slider plate, cylinder slider plate is connected by screw with cylinder mounting plate, cylinder mounting plate is welded on the side of fixed clamping plate, camera fixed plate is also connected on the fixed clamping plate, three-jaw cylinder is built-in in the fixed clamping plate, culture dish is placed below three-jaw cylinder.
2. A microbial isolation and inoculation assembly according to claim 1, wherein: Three jaw cylinders are provided with three clamping claws, the included angle of adjacent two clamping claws is 60°, and the culture dish is fixed on the three-jaw cylinder by the clamping claws.
3. A microbial isolation and inoculation assembly according to claim 1, wherein: A camera is installed on the camera fixed plate, and the camera positions the bacterial colony in the culture dish.
4. A microbial isolation and inoculation assembly according to claim 3, wherein: After the camera positions the bacterial colony in 3D, the cylinder slider plate moves downward, and the inoculation needle dips the target bacterial colony positioned in the culture dish; then the cylinder slider moves upward, the three-jaw cylinder releases the clamping claws, moves above the culture dish, the clamping claws grab the culture dish downward, and then lift the culture dish to move to the storage position; the clamping claws grab the clean culture dish from the incoming material to the scribing position, the three-jaw cylinder is released, the camera positions the bacterial colony again in 3D, and the above steps are repeated to continuously complete the inoculation work.
5. A microbial isolation and inoculation assembly according to claim 1, wherein: The inoculation needle is arranged at an inclined angle, the end of the inoculation needle close to the culture dish is low, and the end of the inoculation needle away from the culture dish is high.