Micro-fluidic chip

Abstract

The utility model relates to the technical field of micro-fluidic chips, in particular to a micro-fluidic chip which comprises a chip main body, a sample injection hole, a flow channel, a reaction hole and an air injection hole, the sample injection hole, the flow channel and the reaction hole are positioned on the chip main body, the sample injection hole, the flow channel and the reaction hole are sequentially connected, and the air injection hole is communicated with the flow channel. The flow channel comprises a sample injection flow channel and a driving flow channel, the sample injection hole, the sample injection flow channel, the flow blocking part, the driving flow channel and the reaction hole are sequentially connected, an inner cavity of the flow blocking part is provided with a flow blocking plug capable of preventing the sample injection flow channel from being communicated with the driving flow channel, the flow blocking plug is in sliding connection with the flow blocking part, and the gas injection hole is located in the driving flow channel. A sealing plug is slidably connected into the gas injection hole. According to the micro-fluidic chip disclosed by the utility model, when the sample liquid in the flow channel is driven to advance by extruding the gas, the gas can be prevented from flowing back in the flow channel, and the volume of the sample liquid entering the reaction area can be obtained through the volume of the gas injected into the flow channel, so that the quantitative control of the sample liquid is realized.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of microfluidic chip, more particularly to a microfluidic chip. BACKGROUND

[0002] Current microfluidic chips are widely used in the fields of biological analysis, medical diagnosis and chemical analysis, and have important value in the scene of rapid detection of trace samples. The existing microfluidic chip usually includes a microfluidic channel, a sample inlet and a plurality of reaction pools, which uses capillary action and external extrusion gas to push the sample liquid into the reaction area to complete the analysis process.

[0003] However, when the existing microfluidic chip uses external gas to push the liquid in the microfluidic channel forward, it is difficult to control the advancing direction of the gas after the gas is extruded into the channel, and the gas is prone to backflow in the channel. At this time, the gas is difficult to push the sample liquid into the reaction area, affecting the accuracy of the analysis result. In order to avoid the backflow of the gas and successfully drive the sample liquid into the reaction area, an excessive amount of gas is usually extruded into the microfluidic channel, and the excessive amount of sample liquid is prone to enter the reaction area after the excessive amount of gas is extruded into the microfluidic channel, which also affects the accuracy of the analysis result. Therefore, when using external gas to extrude the sample liquid in the channel to advance, how to avoid the backflow of the gas and complete the quantitative control of the sample liquid is a problem that needs to be solved to improve the liquid transmission efficiency and detection accuracy of the microfluidic chip. SUMMARY

[0004] The utility model aims at overcoming the insufficient that the sample liquid is difficult to be quantitatively controlled due to the backflow of the gas when the external gas is used to extrude the sample liquid in the microfluidic channel to advance in the prior art, and provides a microfluidic chip. The microfluidic chip in the scheme can avoid the backflow of the gas and realize the quantitative control of the sample liquid when extruding the gas to drive the sample liquid in the channel to advance.

[0005] To solve the above technical problems, the utility model adopts the technical scheme of:

[0006] A microfluidic chip is provided, which includes a chip main body, a sample inlet, a flow channel, a reaction hole and a gas injection hole located on the chip main body, the sample inlet, the flow channel and the reaction hole are connected in sequence, and the gas injection hole is in communication with the flow channel. It also includes a flow interception part, the flow channel includes a sample inlet flow channel and a driving flow channel, the sample inlet, the sample inlet flow channel, the flow interception part, the driving flow channel and the reaction hole are connected in sequence, the inner cavity of the flow interception part is provided with a flow interception plug that can prevent the sample inlet flow channel and the driving flow channel from communicating, the flow interception plug is in sliding connection with the flow interception part, the gas injection hole is located on the driving flow channel, and a sealing plug is in sliding connection in the gas injection hole.

[0007] Before the microfluidic chip of this invention propels the liquid in the flow channel forward via gas, it first moves a flow-blocking plug within the flow-blocking section, preventing the sample inlet channel and the drive channel from connecting. At this time, the sealing plug in the gas injection port moves downwards, forcing the gas from the injection port into the drive channel. Theoretically, after being forced into the drive channel, the gas can move towards the reaction port or the sample inlet channel. However, because the connection between the sample inlet channel and the drive channel is blocked by the flow-blocking plug, the gas entering the drive channel needs to overcome greater pressure to move towards the sample inlet channel. Therefore, the gas entering the drive channel moves towards the reaction port, and the sample liquid in the drive channel passes through the reaction port and enters the reaction zone. Since all the gas entering the drive channel is used to propel the sample liquid forward, the volume of liquid entering the channel is the same as the volume of sample liquid entering the reaction zone. The volume of gas injected into the drive channel can be controlled by controlling the position of the sealing plug in the injection port, thereby controlling the volume of sample liquid entering the reaction zone.

[0008] This novel microfluidic chip, when propelling sample liquid forward through a flow channel by compressed gas, prevents backflow of gas within the channel. Furthermore, it allows for quantitative control of the sample liquid volume entering the reaction zone by controlling the volume of gas injected into the channel. Simultaneously, the design of the flow-blocking section and gas injection port also serves as pressure buffering and multi-dimensional pressure relief. When external pressure changes abruptly during storage and transportation, the internal liquid pressure can be regulated through the gas inside the flow-blocking section and gas injection port, ensuring the stability of liquid storage and flow within the chip and preventing liquid leakage due to vibration or pressure variations.

[0009] Furthermore, the inner cavity of the intercepting part is provided with a limiting platform. The bottom of the limiting platform is provided with an inlet channel and an intercepting channel. The sample inlet channel, the inlet channel, the intercepting channel, and the driving channel are sequentially connected. An insertion hole is provided between the limiting platform and the inner wall of the intercepting part. The bottom of the insertion hole is connected to the intercepting channel. The intercepting plug includes a main body and a branch. The branch is fixedly connected to the main body. When the bottom surface of the main body abuts against the top surface of the limiting platform, the branch passes through the insertion hole and blocks the intercepting channel. When the intercepting plug prevents the sample inlet channel and the driving channel from connecting, it pushes the intercepting plug to slide within the intercepting part until the bottom surface of the intercepting plug abuts against the top surface of the limiting platform. At this point, it is impossible to push the intercepting plug downwards further, as the branch has already passed through the insertion hole and blocked the intercepting channel. After the intercepting plug has prevented the sample inlet channel and the driving channel from connecting, the limiting platform prevents the intercepting plug from moving further downwards.

[0010] Further, the bottom surface of the branch is an inclined bottom surface, which is inclined towards the top surface of the limiting table on the side close to the sample inlet channel. The branch has an inclined bottom surface inclined towards the top surface of the limiting table. After the branch extends into the interception channel, the inclined bottom surface can squeeze the sample liquid in the interception channel to move in the direction of the inlet channel, avoiding the sample liquid in the interception channel from being squeezed into the driving channel during the process of blocking the interception channel, avoiding the quantitative error in the sampling process, ensuring the sample liquid to flow into the reaction zone with accurate amount, and improving the accuracy of detection.

[0011] Further, the driving channel has a plurality of driving channels, each of which is connected with one reaction hole and one gas injection hole. The inlet channel and the interception channel also have a plurality of channels. The plurality of inlet channels are arranged in a circle with the end of the sample inlet channel as the center. The two ends of the interception channel are respectively connected with one inlet channel and one driving channel. The driving channel is provided with a plurality of driving channels, which can simultaneously perform several detections, further improving the detection efficiency of the microfluidic chip.

[0012] Further, the side wall of the interception part is provided with a guide groove for accommodating the branch. The branch is in sliding connection with the guide groove, and the side wall of the guide groove is in close contact with the branch. The bottom surface of the branch is provided with a chamfer. The guide groove is provided to align the branch with the interception channel at the bottom of the limiting table when the interception plug is placed in the interception part. The chamfer on the bottom surface of the branch makes it easier to insert the branch into the guide groove.

[0013] Further, the driving channel includes a first channel and a second channel. The side wall of the gas injection hole is provided with a flow guide port. The first channel and the second channel are respectively connected with the gas injection hole through the flow guide port. The interception channel, the first channel, the gas injection hole and the second channel are connected in sequence. The second channel is S-shaped, and the first channel is linear. The gas in the gas injection part enters the second channel through the flow guide port, and drives the sample liquid in the second channel to move forward in the second channel. The second channel is S-shaped, which can further lengthen the length of the second channel and increase the volume of the sample liquid that can be accommodated in the second channel. The first channel is linear, which can stagger the position of the gas injection hole on the chip body, avoiding interference between the driving of the sealing plug in the gas injection part.

[0014] Further, the interception plug is a silica gel plug, and the sealing plug is a rubber plug. The sealing plug is in interference fit with the gas injection hole. The silica gel material of the interception plug has good elasticity and flexibility, and is not easy to deform. The sealing property is good when the interception channel is blocked. In addition, the silica gel material has stable sealing property, is resistant to high temperature and corrosion, and is not easy to react with the sample liquid in the flow channel. The rubber plug has good elasticity. When the rubber plug is in interference fit with the gas injection part, it can further avoid the leakage of gas in the gas injection part.

[0015] Further, the sample injection hole is provided with a sample injection part connectable with a sample liquid injector, the bottom of the sample injection part is communicated with the sample injection hole, and the bottom of the sample injection part is conical.

[0016] Further, the chip body is further provided with magnetic members and positioning parts, the magnetic members can be adsorbed on a stage of a detection instrument, the magnetic members are arranged at equal intervals around the flow interruption part as a center, the positioning parts are connectable with positioning chips on the stage of the detection instrument, the positioning parts are arranged at equal intervals around the flow interruption part as a center, and the micro-fluidic chip can be produced and processed by an embedded integrated injection molding process to ensure the accuracy of the chip size.

[0017] Compared with the prior art, the micro-fluidic chip has the advantages that:

[0018] When the micro-fluidic chip drives the sample liquid in the flow channel to advance by extruding gas, the gas can be prevented from flowing back in the flow channel, and the volume of the sample liquid entering the reaction area can be obtained by the volume of the gas injected into the flow channel, so that the quantitative control of the sample liquid is realized.

[0019] The limiting table in the flow interruption part of the micro-fluidic chip can remind that the flow interruption plug has stopped the communication between the sample injection flow channel and the driving flow channel, the inclined bottom surface of the branch can extrude the sample liquid in the flow interruption channel to move in the direction of the flow channel, the sample liquid in the flow interruption channel can be prevented from being extruded into the driving flow channel in the process of blocking the flow interruption channel, the quantitative error in the sample injection process can be avoided, the sample liquid can flow into the reaction area with accurate volume, and the detection accuracy is improved.

[0020] The micro-fluidic chip is further provided with magnetic members and positioning parts, the magnetic members can be adsorbed on a stage of a detection instrument, the chip body can be prevented from falling off from the stage of the detection instrument during detection, the positioning parts can detect whether the chip body is accurately positioned at a position to be detected on the stage of the detection instrument, and the accuracy of a detection result can be prevented from being affected by the deviation of the position of the chip. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 Fig. 1 is a structural schematic view of a micro-fluidic chip;

[0022] Figure 2 For Figure 1 A enlarged view of A part of Fig. 1;

[0023] Figure 3 Another angle structural schematic view of a microfluidic chip;

[0024] Figure 4 An internal structure sectional view of a microfluidic chip.

[0025] In the drawings: 1, chip main body; 2, sample inlet hole; 3, reaction hole; 4, gas injection hole; 5, flow blocking part; 6, sample flow channel; 7, driving flow channel; 8, flow blocking plug; 9, sealing plug; 10, limiting platform; 11, flow inlet channel; 12, flow blocking channel; 13, extension hole; 801, main body; 802, branch; 14, guide groove; 701, first flow channel; 702, second flow channel; 15, magnetic member; 16, positioning part; 17, sample inlet part. DETAILED DESCRIPTION

[0026] The utility model will be further described below in combination with specific embodiments. Among them, the drawings are only used for example description, and the representation is only a schematic diagram, and cannot be understood as the limitation of the patent; in order to better illustrate the embodiment of the utility model, some components of the drawings will be omitted, enlarged or reduced, and the size of actual product is not represented; for those skilled in the art, it is understandable that some well-known structures and their description in the drawings can be omitted.

[0027] The same or similar reference numerals in the drawings of the embodiments of the utility model correspond to the same or similar components; in the description of the utility model, it is understood that if the orientation or positional relationship indicated by the terms "upper", "lower", "left", "right" etc. is based on the orientation or positional relationship shown in the drawings, only for the convenience of describing the utility model and simplifying the description, and not indicating or implying that the device or element indicated must have a particular orientation, a particular orientation and operation, therefore the positional relationship description in the drawings is only used for example description, and cannot be understood as the limitation of the patent, for the ordinary skilled in the art, the specific meaning of the above terms can be understood according to the specific situation.

[0028] Embodiment one

[0029] The embodiment is a first embodiment of a microfluidic chip, as shown in Figures 1-4As shown, it comprises a chip body 1, and a sample inlet hole 2, a flow channel, a reaction hole 3 and a gas injection hole 4 on the chip body 1, the sample inlet hole 2, the flow channel and the reaction hole 3 are connected in sequence, and the gas injection hole 4 is communicated with the flow channel. It also comprises a flow blocking part 5, the flow channel comprises a sample inlet flow channel 6 and a driving flow channel 7, the sample inlet hole 2, the sample inlet flow channel 6, the flow blocking part 5, the driving flow channel 7 and the reaction hole 3 are connected in sequence, the inner cavity of the flow blocking part 5 is provided with a flow blocking plug 8 which can prevent the sample inlet flow channel 6 and the driving flow channel 7 from being communicated, the flow blocking plug 8 is slidingly connected with the flow blocking part 5, the gas injection hole 4 is located on the driving flow channel 7, and a sealing plug 9 is slidingly connected in the gas injection hole 4.

[0030] Specifically, the inner cavity of the flow blocking part 5 is provided with a limiting table 10, the bottom of the limiting table 10 is provided with an inlet flow channel 11 and a flow blocking channel 12, the sample inlet flow channel 6, the inlet flow channel 11, the flow blocking channel 12 and the driving flow channel 7 are communicated in sequence, a penetrating hole 13 is arranged between the limiting table 10 and the inner side wall of the flow blocking part 5, and the bottom of the penetrating hole 13 is communicated with the flow blocking channel 12. The flow blocking plug 8 comprises a main body 801 and a branch 802, the branch 802 is fixedly connected with the main body 801, when the bottom surface of the main body 801 abuts against the top surface of the limiting table 10, the branch 802 penetrates through the penetrating hole 13 to block the flow blocking channel 12, and the bottom surface of the branch 802 is an inclined bottom surface which is inclined to the top surface of the limiting table 10 on the side close to the sample inlet flow channel 6.

[0031] The working principle or process of the embodiment is as follows:

[0032] Before the microfluidic chip drives the liquid in the flow channel to advance by gas, the flow blocking plug 8 is first driven to move in the flow blocking part 5 until the bottom surface of the flow blocking plug 8 abuts against the top surface of the limiting table 10, at this time, the flow blocking plug 8 cannot be continuously driven to move downward, and the branch 802 has penetrated through the penetrating hole 13 to block the flow blocking channel. At this time, the sealing plug 9 in the gas injection hole 4 is driven to move downward, and the gas in the gas injection hole 4 is squeezed into the driving flow channel 7 by the downward movement of the sealing plug 9. In theory, the gas squeezed into the driving flow channel 7 can move to the reaction hole 3 or to the sample inlet flow channel 6, but since the communication part between the sample inlet flow channel 6 and the driving flow channel 7 is blocked by the flow blocking plug 8, the gas in the driving flow channel 7 needs to overcome greater pressure when moving to the sample inlet flow channel 6, so the gas in the driving flow channel 7 will move to the reaction hole 3, and the sample liquid in the driving flow channel 7 enters the reaction zone after passing through the reaction hole 3. Since the gas entering the driving flow channel 7 is used to drive the sample liquid to advance, the volume of the liquid in the flow channel is the volume of the sample liquid in the reaction zone, and the volume of the gas injected into the driving flow channel 7 can be controlled by controlling the position of the sealing plug 9 in the gas injection hole 4, so as to control the volume of the sample liquid in the reaction zone.

[0033] The beneficial effects of the embodiment are as follows:

[0034] The microfluidic chip of the embodiment can avoid backflow of gas in the flow channel when advancing the sample liquid in the flow channel by extruding gas, and can also obtain the volume of the sample liquid entering the reaction zone by the volume of the gas injected into the flow channel, so as to realize quantitative control of the sample liquid. Meanwhile, the setting of the flow blocking part 5 and the gas injection hole 4 can also serve the purpose of pressure buffering and multidimensional pressure relief. When the pressure of the microfluidic chip changes suddenly during storage and transportation, the liquid in the microfluidic chip can be adjusted in pressure by the gas in the flow blocking part 5 and the gas injection hole 4, so as to ensure the stability of the liquid storage and flow in the chip and avoid the liquid flowing out of the chip due to vibration or pressure change.

[0035] After the flow blocking plug 8 has blocked the communication between the sample inlet flow channel 6 and the driving flow channel 7, the setting of the limiting table 10 can prevent the flow blocking plug 8 from moving further downward. The bottom surface of the branch 802 is an inclined bottom surface inclined toward the top surface of the limiting table 10. After entering the flow blocking channel 12, the inclined bottom surface can extrude the sample liquid in the flow blocking channel 12 to move in the direction of the inflow channel 11, so as to avoid extruding the sample liquid in the flow blocking channel 12 into the driving flow channel 7 during the process of blocking the flow blocking channel 12, avoid quantitative error during the sample inlet process, ensure the sample liquid to flow into the reaction zone with accurate amount, and improve the accuracy of detection.

[0036] Embodiment Two

[0037] The second embodiment of the microfluidic chip is shown in Figures 1-4 The second embodiment of the microfluidic chip is shown in

[0038] Specifically, the driving flow channel 7 has three, each of which is connected with a reaction hole 3 and a gas injection hole 4, and the inflow channel 11 and the flow blocking channel 12 also have three. The three inflow channels 11 are arranged in a circle with the end of the sample inlet flow channel 6 as the center, and the included angle between adjacent two inflow channels 11 and the included angle between the inflow channel 11 and the sample inlet flow channel 6 are both 90°. The two ends of the flow blocking channel 12 are respectively communicated with one inflow channel 11 and one driving flow channel 7, and the axis of the flow blocking channel is arc-shaped.

[0039] Specifically, the side wall of the flow blocking part 5 is provided with a guide groove 14 for accommodating the branch 802, the branch 802 is slidingly connected with the guide groove 14, the side wall of the guide groove 14 is attached to the branch 802, and the bottom surface of the branch 802 is provided with a chamfer.

[0040] Specifically, the drive flow channel 7 includes a first flow channel 701 and a second flow channel 702, the side wall of the gas injection hole 4 is provided with a flow guide opening, the first flow channel 701 and the second flow channel 702 are communicated with the gas injection hole 4 through the flow guide opening respectively, and the intercept flow channel, the first flow channel 701, the gas injection hole 4 and the second flow channel 702 are connected in sequence. The second flow channel 702 is S-shaped, and the first flow channel 701 is linear.

[0041] The beneficial effects of the embodiment are as follows:

[0042] The three drive flow channels 7 can simultaneously perform three detections, further improving the detection efficiency of the microfluidic chip. The axis of the intercept flow channel is arc-shaped, which can further stagger the three drive flow channels 7, so that the layout of the chip is more reasonable. The setting of the guide groove 14 is more convenient for aligning the branch 802 with the intercept flow channel at the bottom of the limiting table 10 when the intercept plug 8 is placed in the intercept part 5. The bottom surface of the branch 802 is provided with a chamfer, which is more convenient for inserting the branch 802 into the guide groove 14. The second flow channel 702 is S-shaped, which can further lengthen the length of the second flow channel 702 and increase the volume of the sample liquid that the second flow channel 702 can accommodate. The first flow channel 701 is linear, which can stagger the positions of the gas injection holes 4 on the chip main body 1, avoiding interference between the drive gas injection parts when the sealing plugs 9 move.

[0043] Embodiment three

[0044] The third embodiment of the microfluidic chip is shown in Figures 1-4 The structure of the chip main body 1 is further limited based on the first embodiment and the second embodiment.

[0045] Specifically, the intercept plug 8 is a silica gel plug, and the sealing plug 9 is a rubber plug. The sealing plug 9 is in interference fit with the gas injection hole 4.

[0046] Specifically, the sample injection hole 2 is provided with a sample injection part 17 connectable with a sample liquid injector. The bottom of the sample injection part 17 is communicated with the sample injection hole 2, and the bottom of the sample injection part 17 is tapered.

[0047] Specifically, the microfluidic chip can be produced and processed by embedded integrated injection molding process. The chip main body 1 is further provided with a magnetic part 15 and a positioning part 16. The magnetic part 15 can be adsorbed on the object table of the detection instrument. The magnetic part 15 has a plurality of magnetic parts 15, which are arranged in a circle with the intercept part 5 as the center on the chip main body 1. The positioning part 16 can be connected with the positioning chip on the object table of the detection instrument. The positioning part 16 has a plurality of positioning parts 16, which are arranged in a circle with the intercept part 5 as the center on the chip main body 1. The positioning part 16 is a positioning sensor, which can be connected with the positioning chip on the object table of the detection instrument. When the positioning sensor is connected with the positioning chip, it indicates that the position of the chip on the object table of the detection instrument is accurate.

[0048] The beneficial effects of the embodiment are as follows:

[0049] The silicon rubber material of the intercepting plug 8 has good elasticity and flexibility, is not easy to deform, has good sealing performance when blocking the intercepting channel. In addition, the sealing plug 9 of the silicon rubber material is stable in property, resistant to high temperature and corrosion, and is not easy to react with the sample liquid in the flow channel. The rubber plug has good elasticity, and when the rubber plug is in interference fit with the gas injection part, the gas in the gas injection part can be further prevented from leaking out. The sample injection part 17 can be connected to the sample liquid injector, so that the sample solution can be injected into the flow channel through the sample liquid injector. The bottom of the sample injection part 17 is conical, which can prevent the sample liquid from remaining in the sample injection part 17. The microfluidic chip can be produced and processed by embedded integrated injection molding process, which can ensure the accuracy of the size of the chip. The magnetic part 15 can prevent the chip body 1 from falling off the sample stage of the detection instrument during detection. The positioning part 16 can detect whether the chip body 1 is accurately positioned at the position to be detected when the chip body 1 is located on the sample stage of the detection instrument, so as to avoid the deviation of the chip position and affect the accuracy of the detection result.

[0050] In the specific contents of the above specific embodiments, each technical feature can be combined arbitrarily without contradiction. In order to make the description simple, all possible combinations of the above technical features are not described, but as long as the combination of these technical features does not exist contradiction, it should be considered as the scope of the present application.

[0051] Obviously, the above embodiments of the present application are only examples for clearly illustrating the present application, and are not intended to limit the embodiments of the present application. For those skilled in the art, other different forms of changes or modifications can be made on the basis of the above description. Here, all the embodiments are not required to be exhausted. Any modification, equivalent replacement and improvement made within the spirit and principle of the present application shall be included in the protection scope of the claims of the present application.

Claims

1. A microfluidic chip comprising a chip body (1) and a sample inlet hole (2), a flow channel, a reaction hole (3) and a gas injection hole (4) on the chip body (1), the sample inlet hole (2), the flow channel and the reaction hole (3) being connected in sequence, and the gas injection hole (4) communicating with the flow channel, characterized in that, Also include the intercepts (5), the flow channel includes sample flow channel (6) and drive flow channel (7), the sample hole (2), the sample flow channel (6), the intercepts (5), the drive flow channel (7) and the reaction hole (3) are sequentially connected, the inner cavity of the intercepts (5) is provided with the intercept plug (8) that can prevent the sample flow channel (6) and the drive flow channel (7) are communicated, the intercept plug (8) is slidably connected with the intercepts (5), the gas injection hole (4) is located on the drive flow channel (7), the gas injection hole (4) is slidably connected with the sealing plug (9).

2. The microfluidic chip according to claim 1, wherein, The inner cavity of the intercepts (5) is provided with a limiting table (10), the bottom of the limiting table (10) is provided with an inflow channel (11) and an intercept channel (12), the sample flow channel (6), the inflow channel (11), the intercept channel (12) and the drive flow channel (7) are sequentially communicated, the limiting table (10) and the inner side wall of the intercepts (5) are provided with a protruding hole (13), the bottom of the protruding hole (13) is communicated with the intercept channel (12), The intercept plug (8) includes a main body (801) and a branch (802), the branch (802) is fixedly connected with the main body (801), when the bottom surface of the main body (801) abuts against the top surface of the limiting table (10), the branch (802) passes through the protruding hole (13) and blocks the intercept channel (12).

3. The microfluidic chip of claim 2, wherein, The bottom surface of the branch (802) is an inclined bottom surface, which is inclined to the top surface of the limiting table (10) on the side close to the sample flow channel (6).

4. The microfluidic chip of claim 2, wherein, The drive flow channel (7) has a plurality of, each drive flow channel (7) is connected with a reaction hole (3) and a gas injection hole (4), the inflow channel (11) and the intercept channel (12) also have a plurality of, a plurality of inflow channels (11) are arranged in a circle around the end of the sample flow channel (6), and the two ends of the intercept channel (12) are communicated with one inflow channel (11) and one drive flow channel (7) respectively.

5. The microfluidic chip of claim 2, wherein, The side wall of the intercepts (5) is provided with a guide groove (14) for accommodating the branch (802), the branch (802) is slidably connected with the guide groove (14), and the side wall of the guide groove (14) is attached to the branch (802), and the bottom surface of the branch (802) is provided with a chamfer.

6. The microfluidic chip of claim 2, wherein, The drive flow channel (7) includes a first flow channel (701) and a second flow channel (702), the side wall of the gas injection hole (4) is provided with a flow guide opening, the first flow channel (701) and the second flow channel (702) are communicated with the gas injection hole (4) through the flow guide opening respectively, and the intercept channel (12), the first flow channel (701), the gas injection hole (4) and the second flow channel (702) are sequentially connected.

7. The microfluidic chip of claim 6, wherein, The second flow channel (702) is S-shaped, and the first flow channel (701) is linear.

8. The microfluidic chip of claim 1, wherein, The intercept plug (8) is a silica gel plug, the sealing plug (9) is a rubber plug, and the sealing plug (9) is interference fit with the gas injection hole (4).

9. The microfluidic chip of claim 1, wherein, The sample injection hole (2) is provided with a sample injection part (17) connectable with a sample liquid injector, the bottom of the sample injection part (17) is communicated with the sample injection hole (2), and the bottom of the sample injection part (17) is conical.

10. The microfluidic chip of claim 1, wherein, The chip main body (1) is further provided with a magnetic member (15) and a positioning part (16), the magnetic member (15) can be adsorbed on a stage of a detection instrument, and the positioning part (16) is connectable with a positioning chip on the stage of the detection instrument.

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