Cell lysis buffer separation device

Abstract

The utility model relates to the technical field of cell lysis solution separation, and discloses a cell lysis solution separation device which comprises a separation box, a material conveying pump fixedly mounted at the upper part of the separation box, a recovery pipe communicated with the output end of the material conveying pump, a solution inlet pipe communicated with one end of the recovery pipe, and a treatment box communicated with the left side of the separation box, the connecting assembly is arranged on one side of the separation box and comprises a filtering mechanism arranged on the left side of the separation box, a separation mechanism is arranged in the separation box, the input end of the material conveying pump communicates with the bottom of the separation box through a connecting pipe, and a liquid outlet pipe communicates with the left side of the treatment box; a valve is arranged at the outer end of the liquid outlet pipe, and one end of the first spring is fixedly connected with the sliding block. The device disclosed by the utility model solves the problem that the separation effect of the subsequent cell lysis solution is influenced due to the fact that filter residues possibly fall into a separation box when a filter membrane is disassembled by an existing device.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell lysate separation technical field, concretely is a cell lysate separation device. BACKGROUND

[0002] Cell lysate is a kind of cell tissue quick lysate for extracting protein, in industry, the cell lysate capacity handled is often very large, a large amount of cell debris generated after breaking bacteria, and the nucleic acid released, protein precipitate etc. can make lysate component very complex viscous, and the pretreatment of lysate in early stage before centrifugation usually adopts the way of filtration separation to carry out lysate clarification.

[0003] According to the cell lysate separation device disclosed by patent publication number CN 218077278 U, the solution to be separated is filled into the filter mechanism inside through the liquid inlet pipe, filtered through the filter membrane, and the filter mechanism between the left side plate and the right side plate is driven to rotate by the driving mechanism, so that the solution inside the filter mechanism is subjected to centrifugal motion, thereby improving the filtering efficiency of the filter membrane. The filtered solution is filled into the filter mechanism again by the circulating mechanism to be filtered multiple times, thereby improving the separation effect of the lysate. By pulling one end of the movable rod, the clamping effect of the left side plate and the right side plate on the filter mechanism is released, thereby facilitating the replacement of the filter membrane. However, when the filter membrane is disassembled, filter residue may fall into the separation tank, thereby affecting the subsequent separation effect of the cell lysate.

[0004] Therefore, we propose a cell lysate separation device to solve the problem. UTILITY MODEL CONTENTS

[0005] The utility model aims at providing a kind of cell lysate separation device, solve the problem raised in the above background technology.

[0006] To achieve the above object, the utility model provides the following technical scheme: a kind of cell lysate separation device, including separation tank;

[0007] Fixed mounting is installed on the feed pump of separation tank upper portion;

[0008] Recycle pipe is communicated and arranged in the output end of feed pump;

[0009] Liquid inlet pipe is communicated and arranged in one end of recycle pipe;

[0010] Processing tank is communicated and arranged in the left side of separation tank;

[0011] And the connecting assembly arranged on one side of the separation box, the connecting assembly comprising a filtering mechanism arranged on the left side of the separation box, the separation box being internally provided with a separation mechanism, the input end of the feeding pump being in communication with the bottom of the separation box through a connecting pipe, the left side of the processing box being in communication with an outlet pipe, and the outer end of the outlet pipe being provided with a valve, and the front of the separation box being sealingly provided with a box door.

[0012] Preferably, the filtering mechanism comprises a mounting groove opened in the processing box, a mounting frame inserted into the groove, a first filter membrane fixedly installed in the mounting frame, a sliding groove opened in the front of the processing box, a sliding rod fixedly connected to the inner wall of the sliding groove, a sliding block slidingly connected to the outer wall of the sliding rod, a first spring fixedly connected to the inner wall of the sliding groove, a limiting rod fixedly connected to the outer side of the sliding block, a clamping rod movably connected to one end of the limiting rod, a pull rod fixedly connected to the outer end of the clamping rod, a second spring fixedly connected to the outer side of the limiting rod, a clamping groove opened in the front of the processing box, and a sealing ring fixedly connected to the inner wall of the processing box and arranged at the gap between the mounting groove and the mounting frame.

[0013] Preferably, the separation mechanism comprises a motor fixedly installed on the left side of the separation box through a mounting bracket, a gear fixedly connected to the output end of the motor, a rotating cylinder rotatably connected to the left side of the separation box through a bearing, a gear block fixedly connected to the outer wall of the rotating cylinder, an installation plate fixedly connected to the inner end of the rotating cylinder, a movable rod movably connected to the right side of the separation box, a handle fixedly connected to the outer end of the movable rod, a connecting block fixedly connected to the inner end of the movable rod, a limiting plate fixedly connected to the outer wall of the movable rod, a hollow column fixedly connected to the inner wall of the separation box, a solid column sleeved in the hollow column, a third spring fixedly connected to the inner wall of the separation box, a positioning groove opened in one side of the installation plate, a positioning block clamped to the inner wall of the positioning groove, an installation ring fixedly connected to the side of the positioning block away from the installation plate, a supporting rod fixedly connected to the side of the installation ring away from the positioning block, and a second filter membrane fixedly connected to one side of the installation ring.

[0014] Preferably, one end of the first spring is fixedly connected to the sliding block, and the outer end of the second spring is fixedly connected to the pull rod. Through the cooperation of the mounting frame, the first filter membrane, the sliding groove, the sliding rod, the sliding block, the first spring, the limiting rod, the clamping rod, the second spring, and the clamping groove, the separated liquid discharged from the separation box can be further filtered, so that the separation effect of the cell lysate is better, and impurities are prevented from falling into the filtrate at the bottom of the separation box during the disassembly and assembly of the second filter membrane, thereby affecting the separation effect. In addition, the first filter membrane is convenient for manual disassembly and cleaning, thereby facilitating subsequent use.

[0015] Preferably, the clamping rod is clamped to the clamping groove, and the inner side of the limiting rod is attached to the outer side of the mounting frame.

[0016] Preferably, the gear is engaged with the gear block, the rotating cylinder is communicated with the liquid inlet pipe, the solid column is fixedly connected with the limiting plate, the third spring is fixedly connected with the limiting plate, and the supporting rod is fixedly connected with the second filter membrane.

[0017] Preferably, the number of the mounting plates is two, which are symmetrically distributed on the two sides of the second filter membrane, and the mounting plate on the right side is rotatably connected with the connecting block through a bearing, and the mounting plate on the left side is provided with a through hole.

[0018] The utility model provides a cell lysate separation device, and the cell lysate separation device has the following beneficial effects:

[0019] (1) the cell lysate separation device, through setting up filter mechanism, under the action of installation frame, first filter membrane, sliding slot, sliding rod, sliding block, first spring, limiting rod, clamping rod, second spring, clamping groove, can further carry out filter treatment to the separated liquid of discharge separation tank, so that the separation effect of cell lysate is better, can prevent the impurity from falling into the filtrate in the bottom of separation tank in the process of dismounting second filter membrane, influence separation effect, and first filter membrane is convenient for manual dismounting and cleaning, thereby facilitating subsequent use;

[0020] (2) the cell lysate separation device, through setting up separation mechanism, under the action of motor, gear, rotating cylinder, gear block, mounting plate, movable rod, connecting block, limiting plate, hollow column, solid column, third spring, positioning groove, positioning block, mounting ring, supporting rod, second filter membrane, cell lysate can carry out centrifugal motion, thereby improve the filtration efficiency of second filter membrane to solution, and second filter membrane is convenient for manual dismounting and cleaning, thereby increase the applicability of device. DRAWINGS

[0021] Figure 1 It is the whole structure schematic diagram of the utility model;

[0022] Figure 2 It is the whole section structure schematic diagram of the utility model;

[0023] Figure 3 It is filter mechanism structure schematic diagram of the utility model;

[0024] Figure 4 It is separation mechanism structure schematic diagram of the utility model;

[0025] Figure 5 This is a partial structural diagram of the separation mechanism of this utility model;

[0026] In the diagram: 1. Separation box; 2. Feed pump; 3. Connecting assembly; 31. Filtration mechanism; 311. Mounting frame; 312. First filter membrane; 313. Slide groove; 314. Slide rod; 315. Slider; 316. First spring; 317. Limiting rod; 318. Snap-fit ​​rod; 319. Second spring; 3110. Snap-fit ​​groove; 32. Separation mechanism; 321. Motor; 322. Gear; 323. Rotating cylinder; 324. Gear block; 325. Mounting plate; 326. Movable rod; 327. Connecting block; 328. Limiting plate; 329. Hollow column; 3210. Solid column; 3211. Third spring; 3212. Positioning groove; 3213. Positioning block; 3214. Mounting ring; 3215. Support rod; 3216. Second filter membrane; 4. Inlet pipe; 5. Processing box; 6. Recovery pipe. Detailed Implementation

[0027] To provide a clearer understanding of the technical features, objectives, and effects of this utility model, the specific embodiments of this utility model are now described with reference to the accompanying drawings.

[0028] Example 1

[0029] like Figures 1-5 As shown, this utility model provides a technical solution: a cell lysis fluid separation device, including a separation chamber 1, a feed pump 2 fixedly installed on the upper part of the separation chamber 1, a recovery pipe 6 connected to the output end of the feed pump 2, an inlet pipe 4 connected to one end of the recovery pipe 6, a processing chamber 5 connected to the left side of the separation chamber 1, and a connecting assembly 3 located on one side of the separation chamber 1. The connecting assembly 3 includes a filter mechanism 31 located on the left side of the separation chamber 1. A separation mechanism 32 is provided inside the separation chamber 1. The input end of the feed pump 2 is connected to the bottom of the separation chamber 1 through a connecting pipe. An outlet pipe is connected to the left side of the processing chamber 5, and a valve is provided at the outer end of the outlet pipe. A door is sealed at the front of the separation chamber 1. The filter mechanism 31 includes a filter mechanism 32 located in the processing chamber. The processing box 5 has an internal mounting slot, into which a mounting frame 311 is inserted. A first filter membrane 312 is fixedly installed inside the mounting frame 311. A sliding groove 313 is provided at the front of the processing box 5. A sliding rod 314 is fixedly connected to the inner wall of the sliding groove 313. A slider 315 is slidably connected to the outer wall of the sliding rod 314. A first spring 316 is fixedly connected to the inner wall of the sliding groove 313. A limiting rod 317 is fixedly connected to the outer side of the slider 315. A snap-fit ​​rod 318 is movably connected to one end of the limiting rod 317. A pull rod is fixedly connected to the outer end of the snap-fit ​​rod 318. A second spring 319 is fixedly connected to the outer side of the limiting rod 317. A slot 3110 is provided at the front of the processing box 5. A sealing ring is fixedly connected to the inner wall of the processing box 5 and is located in the gap between the mounting groove and the mounting frame 311.

[0030] In the embodiment, one end of the first spring 316 is fixedly connected with the sliding block 315, and the outer end of the second spring 319 is fixedly connected with the pull rod. Through cooperation of the mounting frame 311, the first filter membrane 312, the sliding groove 313, the sliding rod 314, the sliding block 315, the first spring 316, the limiting rod 317, the clamping rod 318, the second spring 319 and the clamping groove 3110, the separated liquid discharged from the separation tank 1 can be further filtered, so that the separation effect of the cell lysate is better, and impurities can be prevented from falling into the filtrate at the bottom of the separation tank 1 in the dismounting process of the second filter membrane 3216, thereby affecting the separation effect. In addition, the first filter membrane 312 is convenient for manual dismounting and cleaning, thereby facilitating subsequent use.

[0031] Further, the clamping rod 318 is clamped with the clamping groove 3110, and the inner side of the limiting rod 317 is attached to the outer side of the mounting frame 311.

[0032] When the filtrate after separation is discharged, the valve on the liquid outlet pipe is opened, then the filtrate in the separation tank 1 enters the treatment tank 5, and the filtrate after separation can be further filtered under the action of the first filter membrane 312, so that the separation effect of the cell lysate is better, and impurities can be prevented from falling into the filtrate at the bottom of the separation tank 1 in the dismounting process of the second filter membrane 3216, thereby affecting the separation effect. Further, when the first filter membrane 312 needs to be dismounted, the clamping rod 318 is pulled outward by manually holding the pull rod, so that the clamping rod 318 is separated from the clamping groove 3110. Then, under the action of the elastic force of the first spring 316, the sliding block 315 moves in the sliding groove 313 through the sliding rod 314, so that the limiting rod 317 fixedly connected to the outer side of the sliding block 315 is away from the outer side of the mounting frame 311. Then, the mounting frame 311 can be taken out outward by manual operation, so that the first filter membrane 312 can be cleaned by manual operation, thereby facilitating subsequent use.

[0033] Embodiment 2

[0034] On the basis of the embodiment 1, the preferable embodiment of the cell lysate separation device provided by the utility model like Figures 1 to 5The separation mechanism 32 includes a motor 321 fixedly installed on the left side of the separation box 1 through a mounting bracket, the output end of the motor 321 is fixedly connected with a gear 322, the left side of the separation box 1 is rotatably connected with a rotating cylinder 323 through a bearing, the outer wall of the rotating cylinder 323 is fixedly connected with a tooth block 324, the inner end of the rotating cylinder 323 is fixedly connected with a mounting plate 325, the right side of the separation box 1 is movably connected with a movable rod 326, the outer end of the movable rod 326 is fixedly connected with a handle, the inner end of the movable rod 326 is fixedly connected with a connecting block 327, the outer wall of the movable rod 326 is fixedly connected with a limiting plate 328, the inner wall of the separation box 1 is fixedly connected with a hollow column 329, the hollow column 329 is sleeved with a solid column 3210 in the inside, the inner wall of the separation box 1 is fixedly connected with a third spring 3211, one side of the mounting plate 325 is provided with a positioning groove 3212, the inner wall of the positioning groove 3212 is clamped with a positioning block 3213, the side, away from the mounting plate 325, of the positioning block 3213 is fixedly connected with a mounting ring 3214, the side, away from the positioning block 3213, of the mounting ring 3214 is fixedly connected with a supporting rod 3215, one side of the mounting ring 3214 is fixedly connected with a second filter membrane 3216.

[0035] In the embodiment, the gear 322 is meshed with the tooth block 324, the rotating cylinder 323 is communicated with the liquid inlet pipe 4, the solid column 3210 is fixedly connected with the limiting plate 328, the third spring 3211 is fixedly connected with the limiting plate 328, the supporting rod 3215 is fixedly connected with the second filter membrane 3216, through the cooperation of the motor 321, the gear 322, the rotating cylinder 323, the tooth block 324, the mounting plate 325, the movable rod 326, the connecting block 327, the limiting plate 328, the hollow column 329, the solid column 3210, the third spring 3211, the positioning groove 3212, the positioning block 3213, the mounting ring 3214, the supporting rod 3215 and the second filter membrane 3216, the centrifugal motion of the cell lysate can be realized, thereby improving the filtering efficiency of the second filter membrane 3216 on the solution, and the second filter membrane 3216 is convenient for manual disassembly and cleaning, thereby increasing the applicability of the device.

[0036] Further, the number of the mounting plates 325 is two, which are symmetrically distributed on the two sides of the second filter membrane 3216, and the mounting plate 325 on the right side is rotatably connected with the connecting block 327 through a bearing.

[0037] When the device is in use, the cell lysate can be delivered into the rotating cylinder 323 through the liquid inlet pipe 4, and then can enter the second filter membrane 3216 through the through hole on the surface of the left mounting plate 325, the gear 322 is driven to rotate by the motor 321, and because the gear 322 is meshed with the toothed block 324, the rotating cylinder 323 can drive the mounting plate 325 to rotate synchronously, the solution can be centrifuged, and the filtering efficiency of the second filter membrane 3216 for the solution is improved, and further, when the second filter membrane 3216 needs to be disassembled, the box door is opened by hand, and then the movable rod 326 is pulled outwards, so that the connecting block 327 fixedly connected to the inner end of the movable rod 326 drives the right mounting plate 325 to move outward, the positioning block 3213 and the positioning groove 3212 are separated, and then the mounting ring 3214 can be taken outwards, and the second filter membrane 3216 can be cleaned, so that subsequent use is facilitated.

[0038] Although the embodiments of the present application have been shown and described, it should be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A cell lysate separation device, comprising a separation tank (1); A feed pump (2) fixedly installed on the upper part of the separation tank (1); A recovery pipe (6) connected to the output end of the feed pump (2); A liquid inlet pipe (4) connected to one end of the recovery pipe (6); A treatment tank (5) connected to the left side of the separation tank (1); and the connecting assembly (3) arranged on one side of the separation tank (1), characterized in that: The connecting assembly (3) comprises a filtering mechanism (31) arranged on the left side of the separation tank (1), the separation tank (1) is internally provided with a separation mechanism (32), the input end of the feed pump (2) is communicated with the bottom of the separation tank (1) through a connecting pipe, the left side of the treatment tank (5) is provided with a liquid outlet pipe, and the outer end of the liquid outlet pipe is provided with a valve, and the front of the separation tank (1) is sealingly provided with a tank door.

2. The cell lysate separation device of claim 1, wherein: The filtering mechanism (31) comprises a mounting groove opened in the inside of the treatment tank (5), a mounting frame (311) is inserted into the groove, a first filter membrane (312) is fixedly installed in the inside of the mounting frame (311), a sliding groove (313) is opened in the front of the treatment tank (5), a sliding rod (314) is fixedly connected to the inner wall of the sliding groove (313), a sliding block (315) is slidingly connected to the outer wall of the sliding rod (314), a first spring (316) is fixedly connected to the inner wall of the sliding groove (313), a limiting rod (317) is fixedly connected to the outer side of the sliding block (315), a clamping rod (318) is movably connected to one end of the limiting rod (317), a pull rod is fixedly connected to the outer end of the clamping rod (318), a second spring (319) is fixedly connected to the outer side of the limiting rod (317), a clamping groove (3110) is opened in the front of the treatment tank (5), and a sealing ring is fixedly connected to the inner wall of the treatment tank (5) and arranged at the gap between the mounting groove and the mounting frame (311).

3. The cell lysate separation device of claim 1, wherein: The separating mechanism (32) includes a motor (321) fixedly installed on the left side of the separating box (1) through a mounting frame, a gear (322) fixedly connected to the output end of the motor (321), a rotating cylinder (323) rotatably connected to the left side of the separating box (1) through a bearing, a tooth block (324) fixedly connected to the outer wall of the rotating cylinder (323), an installation plate (325) fixedly connected to the inner end of the rotating cylinder (323), a movable rod (326) movably connected to the right side of the separating box (1), a handle fixedly connected to the outer end of the movable rod (326), a connecting block (327) fixedly connected to the inner end of the movable rod (326), a limiting plate (328) fixedly connected to the outer wall of the movable rod (326), a hollow column (329) fixedly connected to the inner wall of the separating box (1), a solid column (3210) sleeved in the hollow column (329), a third spring (3211) fixedly connected to the inner wall of the separating box (1), a positioning groove (3212) formed in one side of the installation plate (325), a positioning block (3213) clamped to the inner wall of the positioning groove (3212), an installation ring (3214) fixedly connected to the side, away from the installation plate (325), of the positioning block (3213), a supporting rod (3215) fixedly connected to the side, away from the positioning block (3213), of the installation ring (3214), and a second filter membrane (3216) fixedly connected to one side of the installation ring (3214).

4. The cell lysate separation device of claim 2, wherein: One end of the first spring (316) is fixedly connected with the sliding block (315), and the outer end of the second spring (319) is fixedly connected with the pull rod.

5. The cell lysate separation device of claim 2, wherein: The clamping rod (318) is clamped to the clamping groove (3110), and the limiting rod (317) is arranged in abutment with the outer side of the mounting frame (311).

6. The cell lysate separation device of claim 3, wherein: The gear (322) is arranged in meshing with the tooth block (324), the rotating cylinder (323) is arranged in communication with the liquid inlet pipe (4), the solid column (3210) is fixedly connected with the limiting plate (328), the third spring (3211) is fixedly connected with the limiting plate (328), and the supporting rod (3215) is fixedly connected with the second filter membrane (3216).

7. The cell lysate separation device of claim 3, wherein: The number of the installation plates (325) is two, which are symmetrically distributed on the two sides of the second filter membrane (3216), and the installation plate (325) on the right side is rotatably connected with the connecting block (327) through a bearing, and the installation plate (325) on the left side is provided with a through hole.

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