Centrifugal tube for separating nucleated cells and matched adapter set

Abstract

The utility model relates to the field of centrifugal tubes, in particular to a centrifugal tube for separating nucleated cells and a matched adapter set. The centrifugal tube comprises a centrifugal tube body and an adapter arranged in the centrifugal tube body. The adapter comprises a contracted pipe piece, a positioning piece and an abutting piece. The pipe diameter of the centrifugal pipe can be reduced through the through hole formed in the pipe reducing piece, so that the nucleated cell layer is thickened. The positioning piece comprises a positioning block, a sliding plate and a connecting plate; and by pushing the sliding plate, the connecting plates at the two ends are clamped to the upper ends and the lower ends of the two positioning blocks correspondingly, so that the contracted pipe fitting is positioned. According to the utility model, the column body is adjusted between the two vertically adjacent positioning blocks, and the sliding plates are respectively pushed towards two sides, so that the connecting plates at two ends of the sliding plates are respectively clamped at the upper ends and the lower ends of the two positioning blocks, and the positioning of the column body is completed. After centrifugation is completed, a nucleated cell layer appears in the through hole, the inner diameter of the centrifugal tube can be reduced, and the nucleated cell layer is thickened, so that the nucleated cell layer is conveniently and accurately sucked out.

Description

TECHNICAL FIELD

[0001] The utility model relates to centrifugal tube field especially, a kind of centrifugal tube and matching adapter set for separating nucleated cell. BACKGROUND

[0002] Nucleated cell separation is a common biological technology, aimed at obtaining nucleated cells from tissue or cell mixture for further research and analysis. Centrifugal tube is often used in the process of nucleated cell separation, which is a common centrifugal tool in laboratory.

[0003] The Chinese patent document with the authorization announcement number CN221619481U proposes a centrifugal tube, comprising: a centrifugal tube body, a heat preservation part is arranged at the bottom end of the centrifugal tube body, a fixing part is arranged at the bottom end of the heat preservation part, a reinforcing part is arranged at the top end of the centrifugal tube body, a sealing part is detachably connected to the top end of the centrifugal tube body, and the sealing part is matched with the reinforcing part; the fixing part comprises a fixing seat fixed to the bottom end of the heat preservation part, and a fixing assembly is arranged at the bottom end of the fixing seat.

[0004] In the above application, the heat preservation part is fixed above the fixing seat, and the centrifugal tube body is slidingly connected in the heat preservation part, so that the centrifugal tube body is convenient to take and place, and is free from the dependence of the centrifugal tube body on the centrifugal tube support. However, the centrifugal tube design has problems such as complex operation, unclear observation of cell layer, and inaccurate suction of cell layer. UTILITY MODEL CONTENT

[0005] In view of the problems in the background art, a centrifugal tube and matching adapter set for separating nucleated cells are proposed.

[0006] The utility model proposes a centrifugal tube and matching adapter set for separating nucleated cells, comprising a centrifugal tube and an adapter arranged in the centrifugal tube. The adapter comprises a pipe reducing piece, a positioning piece and a pressing piece. The pipe reducing piece can reduce the pipe diameter of the centrifugal tube through the through hole arranged thereon, so as to thicken the nucleated cell layer. The positioning piece comprises positioning blocks, a sliding plate and connecting plates; by pushing the sliding plate, the connecting plates at both ends are respectively clamped at the upper end and the lower end of the two positioning blocks, so as to position the pipe reducing piece. The pressing piece comprises a spring and a pressing block; by pushing the pressing block to abut against the inner wall of the centrifugal tube through the spring, the gap between the pipe reducing piece and the centrifugal tube can be reduced.

[0007] Preferably, the pipe reducing piece comprises a column movably arranged in the centrifugal tube; the column is made of transparent material, its diameter is matched with the inner diameter of the centrifugal tube, and a through hole is arranged on the column.

[0008] Preferably, the positioning piece comprises a plurality of positioning blocks symmetrically arranged at the inner wall of the centrifugal tube; the plurality of positioning blocks on the same side are equidistantly arranged; sliding grooves one are symmetrically arranged on both sides of the column, and the column is slidingly connected with the plurality of positioning blocks through the sliding grooves one.

[0009] Preferably, the upper and lower ends of the plurality of positioning blocks are provided with clamping grooves; the two sides of the through hole are respectively communicated with sliding grooves two; the two sliding grooves two are opposite to the corresponding side of the sliding groove one, and the inner sides of the two sliding grooves two are slidably connected with sliding plates; the upper and lower ends of the sliding plates are connected with connecting plates; the opposite ends of the two connecting plates are provided with clamping blocks; and the clamping blocks are matched and clamped with the clamping grooves.

[0010] Preferably, the upper end of the upper connecting plate is provided with a handle.

[0011] Preferably, the pressing member comprises a plurality of hole grooves uniformly arranged on the side wall of the column; the groove bottom of the hole groove is connected with a spring; and the other end of the spring is connected with a pressing block in sliding connection with the hole groove.

[0012] Preferably, a deformable connecting strip is arranged between the two adjacent pressing blocks.

[0013] Compared with the prior art, the utility model has the following beneficial technical effects:

[0014] (1) the centrifugal tube for separating nucleated cells and the matching adapter set, through adjusting the column between the upper and lower adjacent two positioning blocks, pushing the sliding plates to the two sides respectively, making the connecting plates at the two ends of the sliding plates respectively clamped on the upper end and the lower end of the two positioning blocks, to complete the positioning of the column. After centrifugation, the nucleated cell layer appears in the through hole, the inner diameter of the centrifugal tube can be reduced, so that the nucleated cell layer is thickened, thereby facilitating accurate suction. The separation efficiency and operation convenience of nucleated cells in whole blood are improved, and the utility model has wide application prospect and market value.

[0015] (2) the centrifugal tube for separating nucleated cells and the matching adapter set, through the spring pushing the pressing block and the inner wall of the centrifugal tube, the gap between the column and the centrifugal tube can be reduced, so that the nucleated cell layer can flow into the through hole as much as possible, and the nucleated cell layer is further thickened. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 It is the whole structure schematic view of the utility model;

[0017] Figure 2 It is the pipe reducing piece structure schematic view of the utility model;

[0018] Figure 3 It is the positioning piece structure schematic of the utility model Figure 1 ;

[0019] Figure 4 It is the positioning piece structure schematic of the utility model Figure 2 ;

[0020] Figure 5 It is the pressing piece structure schematic view of the utility model.

[0021] 1, centrifuge tube; 2, adapter; 21, pipe reducing element; 211, column; 212, through hole; 22, positioning element; 221, sliding groove one; 222, positioning block; 223, clamping groove; 224, sliding groove two; 225, sliding plate; 226, connecting plate; 227, clamping block; 228, handle; 23, pressing element; 231, hole slot; 232, spring; 233, pressing block; 234, connecting strip. DETAILED DESCRIPTION

[0022] Example one

[0023] As Figures 1-5 shown, the utility model provides a kind of centrifuge tube and matching adapter set for separating nucleated cell, including centrifuge tube 1 and the adapter 2 being arranged in centrifuge tube 1.Adapter 2 includes pipe reducing element 21, positioning element 22 and pressing element 23.Pipe reducing element 21 is reduced by the through hole 212 being opened on it The pipe diameter of centrifuge tube 1, to thicken nucleated cell layer.Positioning element 22 includes positioning block 222, sliding plate 225 and connecting plate 226;By pushing sliding plate 225, make the connecting plate 226 of two ends respectively clamped in the upper end and lower end of two positioning blocks 222, to carry out the positioning of pipe reducing element 21.Pressing element 23 includes spring 232 and pressing block 233;By spring 232 push pressing block 233 and the inner wall of centrifuge tube 1 are pressed, can reduce the gap between pipe reducing element 21 and centrifuge tube 1.

[0024] Further explanation, pipe reducing element 21 includes column 211 being movably arranged in centrifuge tube 1;Column 211 is transparent material, to facilitate the position and state of nucleated cell layer are observed, its diameter and the inner diameter of centrifuge tube 1 are adapted, and through hole 212 is opened on column 211.Pipe diameter of centrifuge tube 1 can be reduced, so that nucleated cell layer is thickened, to facilitate its accurate suction.

[0025] Further explanation, the positioning member 22 comprises a plurality of positioning blocks 222 symmetrically arranged at the inner wall of the centrifugal tube 1; the plurality of positioning blocks 222 on the same side are equidistantly arranged; the two sides of the column body 211 are symmetrically provided with a plurality of sliding grooves 221, and the plurality of positioning blocks 222 are slidably connected with the plurality of sliding grooves 221. The upper and lower ends of the plurality of positioning blocks 222 are provided with clamping grooves 223; the two sides of the through hole 212 are respectively communicated with sliding grooves 224; the two sliding grooves 224 are opposite to the corresponding sliding grooves 221, and the inner sides of the two sliding grooves 224 are slidably connected with sliding plates 225; the upper and lower ends of the sliding plate 225 are connected with connecting plates 226; the opposite ends of the two connecting plates 226 are provided with clamping blocks 227; the clamping blocks 227 are made of elastic material, specifically rubber; the clamping blocks 227 are clamped with the clamping grooves 223. The height of the column body 211 is equal to the distance between the two positioning blocks 222, the height of the column body 211 is adjusted between the two adjacent positioning blocks 222, the sliding plate 225 is pushed towards the positioning blocks 222, the upper clamping block 227 is clamped with the clamping groove 223 at the upper end of the upper positioning block 222, and the lower clamping block 227 is clamped with the clamping groove 223 at the lower end of the lower positioning block 222, so as to complete the positioning of the column body 211. The position of the column body 211 will not change during centrifugation, and the height of the column body 211 in the centrifugal tube 1 is adjusted in advance, so that the nucleated cell layer can be accurately retained in the through hole 212, and the nucleated cell layer is easily sucked.

[0026] Further explanation, the upper end of the upper connecting plate 226 is provided with a handle 228, so as to facilitate the pushing of the sliding plate 225.

[0027] Further explanation, the pressing member 23 comprises a plurality of hole grooves 231 uniformly arranged at the side wall of the column body 211; the groove bottoms of the hole grooves 231 are connected with springs 232; the other ends of the springs 232 are connected with pressing blocks 233 which are slidably connected with the hole grooves 231; the pressing blocks 233 can be seamlessly attached to the inner wall of the centrifugal tube 1. By pushing the pressing blocks 233 to abut against the inner wall of the centrifugal tube 1 through the springs 232, the gap between the column body 211 and the centrifugal tube 1 can be reduced, so that the nucleated cell layer can flow into the through hole 212 as much as possible, and the nucleated cell layer is further thickened.

[0028] Further explanation, the deformable connecting strips 234 are arranged between the adjacent two pressing blocks 233, which can fill the gap between the pressing blocks 233 by deforming themselves, further reducing the gap between the column body 211 and the centrifugal tube 1.

[0029] The working principle of the utility model is as follows: firstly, the whole blood sample is added into the centrifugal tube 1, then the position of the nucleated cell layer after centrifugation is predicted according to the volume of the whole blood, and the column 211 is adjusted between the two adjacent positioning blocks 222 at the corresponding height. Then the handle 228 is pushed to the two sides respectively, so as to push the slide plate 225, make the upper clamping block 227 cooperate with the clamping groove 223 on the upper end of the upper positioning block 222, and at the same time, the lower clamping block 227 cooperates with the clamping groove 223 on the lower end of the lower positioning block 222, so as to complete the positioning of the column 211. After centrifugation, the nucleated cell layer appears in the through hole 212, the inner diameter of the centrifugal tube 1 can be reduced, so that the nucleated cell layer is thickened, thereby the nucleated cell layer can be accurately sucked out. The separation efficiency of the nucleated cells in the whole blood is improved, and the operation convenience is improved, and the utility model has wide application prospect and market value.

[0030] The above describes the embodiments of the utility model in detail in combination with the drawings, but the utility model is not limited to this, and various changes can be made within the knowledge range of the skilled in the art without departing from the purpose of the utility model.

Claims

1. A centrifuge tube and mating adapter set for isolating nucleated cells, characterized in that, The centrifugal tube (1) and the adapter (2) arranged in the centrifugal tube (1); The adapter (2) comprises a tube reducing part (21), a positioning part (22) and a pressing part (23); The tube reducing part (21) can reduce the tube diameter of the centrifugal tube (1) through the through hole (212) formed on the tube reducing part (21), so as to thicken the nucleated cell layer; The positioning part (22) comprises positioning blocks (222), sliding plates (225) and connecting plates (226); by pushing the sliding plates (225), the connecting plates (226) at both ends are respectively clamped on the upper end and the lower end of the two positioning blocks (222) to position the tube reducing part (21); The pressing part (23) comprises springs (232) and pressing blocks (233); by pushing the pressing blocks (233) to abut against the inner wall of the centrifugal tube (1) through the springs (232), the gap between the tube reducing part (21) and the centrifugal tube (1) can be reduced.

2. The centrifuge tube and mating adapter set for isolating nucleated cells of claim 1, wherein, The tube reducing part (21) comprises a column (211) movably arranged in the centrifugal tube (1); the column (211) is made of transparent material, the diameter of the column (211) is matched with the inner diameter of the centrifugal tube (1), and the through hole (212) is formed on the column (211).

3. The centrifuge tube and mating adapter set for isolating nucleated cells of claim 2, wherein, The positioning part (22) comprises a plurality of positioning blocks (222) symmetrically arranged at the inner wall of the centrifugal tube (1); the plurality of positioning blocks (222) on the same side are equidistantly arranged; the two sides of the column (211) are symmetrically provided with sliding grooves (221), and the column (211) is slidably connected with the plurality of positioning blocks (222) through the sliding grooves (221).

4. The centrifuge tube and mating adapter set for isolating nucleated cells of claim 3, wherein, The upper and lower ends of the plurality of positioning blocks (222) are provided with clamping grooves (223); the two sides of the through hole (212) are respectively connected with sliding grooves (224); the two sliding grooves (224) are opposite to the corresponding sliding grooves (221), and the sliding grooves (224) are slidably connected with sliding plates (225) inside; the upper and lower ends of the sliding plates (225) are connected with connecting plates (226); the opposite ends of the two connecting plates (226) are provided with clamping blocks (227); the clamping blocks (227) are clamped with the clamping grooves (223).

5. The centrifuge tube and mating adapter set for isolating nucleated cells of claim 4, wherein, The upper end of the upper connecting plate (226) is provided with a handle (228).

6. The centrifuge tube and mating adapter set for isolating nucleated cells of claim 2, wherein, The pressing part (23) comprises a plurality of hole grooves (231) uniformly formed on the side wall of the column (211); the groove bottoms of the hole grooves (231) are connected with springs (232); the other ends of the springs (232) are connected with pressing blocks (233) slidably connected with the hole grooves (231).

7. The centrifuge tube and mating adapter set for isolating nucleated cells of claim 6, wherein, The deformable connecting strips (234) are arranged between the adjacent two pressing blocks (233).

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