Cell suspension culture device

Abstract

The utility model relates to a cell suspension culture device which comprises a culture dish used for containing a culture medium; the peristaltic pump is connected to the culture dish through a guide pipe, and the peristaltic pump is used for driving the culture medium to flow. A peristaltic pump is adopted as a power source and is connected with a culture dish through a catheter, a microenvironment similar to the heart and blood vessels in function is established, the catheter and a culture medium in the culture dish are driven to tend to have the same flow speed as blood in the blood vessels through power of the pump, and therefore the problems that liquid splashes and the flow speed of the culture medium is not uniform are solved; and the micro-environment of the human blood flow velocity can be synchronously simulated, so that real cell suspension culture is realized.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture field, concretely relates to a cell suspension culture device. BACKGROUND

[0002] When culturing tumor cells, it is usually divided into two modes of adherent culture and suspension culture, and the suspension culture part is mainly used for researching the growth state of tumor cells under non-adherent condition. At present, the mode of cell suspension culture is usually that tumor cells are loaded into a cell culture dish containing culture medium, and then the culture dish is fixed on a horizontal shaking table, the horizontal shaking table shakes uniformly to drive the tumor cells to move in the culture medium without sedimentation.

[0003] However, this method has two disadvantages: 1. Since the culture dish generally belongs to an open vessel, violent shaking will cause the culture medium to splash, affecting the experimental results; 2. The shaking of the horizontal shaking table is uneven, resulting in uneven flow rate of the culture medium in the culture dish, some positions have fast flow rate and some positions have slow flow rate, which cannot achieve the effect of uniform flow rate and uniform environment, and will also affect the experimental results. SUMMARY

[0004] Based on the above description, the utility model provides a cell suspension culture device to solve the problems of liquid splashing and uneven flow rate of culture medium in the related art.

[0005] The technical scheme for solving the above technical problem is as follows: a cell suspension culture device, comprising: a culture dish for containing culture medium; a peristaltic pump connected to the culture dish through a conduit, the peristaltic pump being used to drive the culture medium to flow.

[0006] On the basis of the above technical scheme, the utility model can also be improved as follows.

[0007] Further, the bottom of the culture dish is provided with two hard conduits, one of the hard conduits is connected to the inlet of the peristaltic pump, and the other hard conduit is connected to the outlet of the peristaltic pump.

[0008] Further, a soft conduit is arranged in the peristaltic pump, and the hard conduit is connected to the soft conduit.

[0009] Further, a filter membrane is arranged at the joint of the hard conduit and the soft conduit.

[0010] Further, a roller is arranged in the peristaltic pump, and the soft conduit is arranged outside the roller.

[0011] Further, the two hard conduits are arranged in parallel and at intervals.

[0012] Further, two hard pipes are respectively connected to two sides of the bottom of the culture dish.

[0013] Further, the peristaltic pump is located below the culture dish.

[0014] Further, the hard pipe is vertically arranged.

[0015] Further, the soft pipe is arranged in a U shape.

[0016] Compared with the prior art, the technical scheme of the application has the following beneficial technical effects:

[0017] By using a peristaltic pump as a power source, and connecting the peristaltic pump and the culture dish by a pipe, a microenvironment similar to the function of a heart and blood vessels is established, and by using the power of the pump, the pipe and the culture medium in the culture dish are driven to have a flow rate similar to that of blood in blood vessels, so that the problems of liquid splashing and uneven flow rate of the culture medium are solved, and a microenvironment similar to the flow rate of human blood is simulated synchronously, and true cell suspension culture is achieved. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 The whole structure schematic view of the cell suspension culture device provided by the embodiment of the present application is provided.

[0019] Figure 2 The cross-sectional view of the cell suspension culture device provided by the embodiment of the present application is provided.

[0020] In the drawings, the component list represented by each reference sign is as follows:

[0021] 1, culture medium; 2, culture dish; 3, hard pipe; 4, filter membrane; 5, soft pipe; 6, peristaltic pump; 7, roller. DETAILED DESCRIPTION

[0022] In order to facilitate the understanding of the present application, the present application will be described more fully below with reference to the related drawings. The embodiments of the present application are shown in the drawings. However, the present application can be realized in many different forms, and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive.

[0023] The embodiment of the present application provides a cell suspension culture device, which can solve the problems of liquid splashing and uneven flow rate of the culture medium in the related art.

[0024] Referring to Figure 1As shown in the utility model embodiment provides a kind of cell suspension culture device, it includes: culture dish 2, for containing culture medium 1;Peristaltic pump 6 is connected to the culture dish 2 by conduit, the peristaltic pump 6 is used to drive the culture medium 1 flow, in this embodiment, by combining heart pacing as power, to the principle phenomenon of uniform control of blood flow rate, using the power of peristaltic pump 6 simulates the heart pacing power source, provides uniform flow rate to the culture medium 1 in culture dish 2, reaches the effect that neither can cause liquid splashing, and can stabilize culture medium 1 flow rate, to truly achieve cell suspension culture.

[0025] participate Figure 1 As shown in the utility model embodiment, in some embodiments, the bottom of the culture dish 2 is provided with two hard pipes 3, one of the hard pipes 3 is connected to the inlet of the peristaltic pump 6, and the other hard pipe 3 is connected to the outlet of the peristaltic pump 6, in this embodiment, by setting hard pipe 3 connected to peristaltic pump 6 at the bottom of culture dish 2, hard pipe 3 can be used as a hard support for culture dish 2, to avoid shaking or splashing of culture medium 1.

[0026] participate Figure 1 As shown in the utility model embodiment, in some embodiments, a soft pipe 5 is arranged in the peristaltic pump 6, and the hard pipe 3 is connected to the soft pipe 5, by arranging the soft pipe 5, the peristaltic pump 6 is elastically connected with the hard pipe 3, so that the peristaltic pump 6 has a certain vibration space when working, facilitating the peristaltic pump 6 to drive the liquid flow.

[0027] participate Figure 1 As shown in the utility model embodiment, in some embodiments, a filter membrane 4 is arranged at the joint of the hard pipe 3 and the soft pipe 5, by connecting the filter membrane 4 between the hard pipe 3 and the soft pipe 5, the filter membrane 4 can prevent cells from flowing into the peristaltic pump 6, reducing the damage of the peristaltic pump 6 to the cells.

[0028] participate Figure 2 As shown in the utility model embodiment, in some embodiments, a roller 7 is arranged in the peristaltic pump 6, and the soft pipe 5 is arranged outside the roller 7, the roller 7 is driven to rotate by a motor, and the roller 7 applies pressure to the soft pipe 5, so that the culture medium 1 flows.

[0029] participate Figure 1 As shown in the utility model embodiment, in some embodiments, the two hard pipes 3 are arranged in parallel and spaced apart, reducing the moving distance of the culture medium 1, and facilitating the uniform flow of the culture medium 1.

[0030] participate Figure 1 As shown in the utility model embodiment, in some embodiments, the two hard pipes 3 are respectively connected to the bottom of the culture dish 2 on both sides, by arranging the hard pipes 3 on both sides, the culture medium 1 is automatically stirred evenly in the process of flowing in the culture dish 2.

[0031] participateFigure 1 As shown, in some embodiments, the peristaltic pump 6 is located below the culture dish 2, facilitating the flow of the culture medium 1 under the assistance of gravity, shortening the moving path.

[0032] Participation Figure 1 As shown, in some embodiments, the hard conduit 3 is vertically arranged, facilitating the flow of the culture medium 1, reducing the resistance in the flow process.

[0033] Participation Figure 1 As shown, in some embodiments, the soft conduit 5 is arranged in a U shape, and the soft conduit 5 is arranged around the outer side of the roller 7, facilitating the rotation of the roller 7 in both positive and reverse directions to extrude the soft conduit 5.

[0034] When the device starts to operate, the motor is first driven to rotate in the positive direction, thereby driving the roller 7 in the peristaltic pump 6 to rotate in the positive direction, extruding the soft conduit 5, so that the culture medium 1 in the culture dish 2, the hard conduit 3 and the soft conduit 5 obtains a speed close to the blood flow rate. At this time, when the tumor cells in the culture medium reach the right filter membrane 4, the filter membrane 4 traps the tumor cells, so that the cells cannot flow into the soft conduit 5 in the peristaltic pump 6. Conversely, the motor is driven to rotate in the reverse direction, and the culture medium flows uniformly in the reverse direction, driving the tumor cells trapped by the right filter membrane 4 to move to the left filter membrane 4 in the reverse direction, and being trapped by the filter membrane 4 after reaching the position. This method can effectively avoid the problem of splashing of the culture medium, and can make the culture medium obtain a uniform and effective speed close to the blood flow rate in the entire device, so that the cells truly reach the suspension culture condition.

[0035] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used in the description herein is for describing specific embodiments only and is not intended to be limiting of the application.

[0036] It is to be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientations depicted in the figures. For example, if a device described herein is turned over in use, a relative preposition such as "below" can be interpreted as "above" or "up" and vice versa. Such a change in spatial relative terms can be necessary to account for the different orientations of the device in use or operation. Therefore, the exemplary terms "below" and "above" can encompass both an orientation of above and below. Moreover, the devices can be otherwise oriented (for example, rotated 90 degrees or at other orientations) and the spatial descriptions used herein interpreted accordingly.

[0037] It should be noted that when one element is considered to be "connected" to another element, it can be directly connected to the other element or connected to the other element through an intermediary element. In the following embodiments, "connection" should be understood as "electrical connection," "communication connection," etc., if the connected circuits, modules, units, etc., have the transmission of electrical signals or data between them.

[0038] When used herein, the singular forms of “a,” “an,” and “the” may also include the plural forms unless the context clearly indicates otherwise. It should also be understood that the terms “comprising,” “including,” or “having,” etc., specify the presence of the stated feature, whole, step, operation, component, part, or combination thereof, but do not preclude the possibility of the presence or addition of one or more other features, wholes, steps, operations, components, parts, or combinations thereof.

[0039] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A cell suspension culture device, characterized by, It comprises: a culture dish (2) for containing culture medium (1); a peristaltic pump (6) connected to the culture dish (2) by a conduit, the peristaltic pump (6) being used to drive the culture medium (1) to flow.

2. The cell suspension culture device according to claim 1, wherein: two hard conduits (3) are installed on the bottom of the culture dish (2), one of the hard conduits (3) being connected to the inlet of the peristaltic pump (6) and the other being connected to the outlet of the peristaltic pump (6).

3. The cell suspension culture device according to claim 2, wherein: a soft conduit (5) is arranged in the peristaltic pump (6), and the hard conduit (3) is connected to the soft conduit (5).

4. The cell suspension culture device according to claim 3, wherein: a filter membrane (4) is installed at the joint of the hard conduit (3) and the soft conduit (5).

5. The cell suspension culture device according to claim 3, wherein: a roller (7) is installed inside the peristaltic pump (6), and the soft conduit (5) is arranged outside the roller (7).

6. The cell suspension culture device according to claim 2, wherein: the two hard conduits (3) are arranged in parallel and at intervals.

7. The cell suspension culture device according to claim 6, wherein: the two hard conduits (3) are respectively connected to the two sides of the bottom of the culture dish (2).

8. The cell suspension culture device according to claim 2, wherein: the peristaltic pump (6) is located below the culture dish (2).

9. The cell suspension culture device according to claim 8, wherein: the hard conduits (3) are arranged vertically.

10. The cell suspension culture device according to claim 3, wherein: the soft conduit (5) is arranged in a U shape.

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