Mouse small intestine epithelial cell culture device

By designing a rat intestinal epithelial cell culture apparatus with components such as scaffolds, channels, and pores, the operation process of the culture medium is simplified, solving the problem of cumbersome culture medium replacement and cleaning operations in existing technologies. This improves efficiency and experimental accuracy. In particular, by simplifying the experimental operation and the cell culture apparatus, a highly efficient cell culture apparatus is achieved. This simplifies the experimental operation process, improves the efficiency of culture medium replacement and cleaning, avoids cell damage, and ensures the cleanliness of the cell culture environment and the accuracy of the experiment.

CN223723140UActive Publication Date: 2025-12-26PUER PEOPLES HOSPITAL
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Patent Information

Application Number
CN202423142636.8
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-12-19
Publication Date
2025-12-26
Estimated Expiration
2034-12-19

AI Technical Summary

Technical Problem

In existing technologies, the culture medium replacement and washing operations during the culture of rat small intestinal epithelial cells are cumbersome, require high skills and patience, and are prone to damaging the cells.

Method used

A rat small intestinal epithelial cell culture device was designed, comprising a scaffold, fluid channels, fluid pores, piston, screw, wire sleeve, and fluid extraction/distraction tubing. Through the coordinated work of these components, the culture medium and cleaning solution can be rapidly aspirated and injected, simplifying the operation process and reducing contact with cells.

Benefits of technology

It improves the efficiency of culture medium replacement and cleaning, avoids cell damage, ensures the cleanliness of the cell culture environment and the accuracy of experiments, and saves researchers' time and effort.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses a mouse small intestine epithelial cell culture device which comprises a culture structure, the culture structure comprises a culture dish, a central support on the lower surface of the culture dish is connected with a tray, and the tray is bonded with a film. A liquid channel in the support is provided with a liquid hole communicated with the culture dish, a piston in the liquid channel is connected with a film and a screw penetrating out of the liquid channel, and a liquid channel port screw sleeve is in threaded transmission with the screw and is provided with a liquid drainage pipeline and a connector. When the culture medium is replaced, the culture dish is moved to an ultra-clean workbench, a liquid pumping and discharging pipeline and pumping equipment are connected, a screw rod is rotated to enable a piston to unseal a liquid hole, a new culture medium can be rapidly pumped and injected, operation is easy and convenient, complexity is reduced, cell damage is prevented, efficiency and success rate are improved, and the device is suitable for frequent liquid replacement experiments. When cells are cleaned, a liquid drainage pipeline and cleaning liquid equipment are connected, cleaning liquid is injected and extracted through similar operations, the cleaning liquid uniformly acts on the periphery of the cells through liquid channels and liquid holes, cleaning is more thorough and efficient, the environment is kept clean, the influence of impurities is reduced, and it is guaranteed that experiments are accurate and reliable.
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Description

TECHNICAL FIELD

[0001] The utility model relates to experimental apparatus technical field, concretely is a mouse small intestine epithelial cell culture appliance. BACKGROUND

[0002] Bone morphogenetic protein is transforming growth factor beta (TGF-beta) family growth factor, and the main member of BMP in the intestinal tract includes BMP2 and BMP4, and at present, in the research experiment, mouse is used as the model, through the research of the change of the BMP2 / BMP4 signal path and related member of the young mouse, adult mouse, old mouse small intestine epithelial cell, aims at elucidating the function and mechanism of the BMP2 / BMP4 signal path in the mouse small intestine epithelial cell senescence, provides new theoretical basis for delaying senescence in clinic.

[0003] In actual scientific research experiment scene, the culture work of mouse small intestine epithelial cell usually carries out with the help of culture dish. Generally speaking, the conventional culture dish when cultivating this kind of cell, due to the demand of cell growth metabolism, often changes once every 2 to 3 days, so as to ensure that the culture medium can provide enough nutrients for the cell, maintains its normal growth, proliferation and physiological activities. And this culture medium replacement operation process is more complicated, needs the operator to use the pipette, very carefully in the culture dish edge carries out the old culture medium suction removal work, then accurately injects the newly prepared culture medium into the culture dish. Moreover, when washing mouse small intestine epithelial cell, the steps and ways are basically the same, also need to use the pipette in the culture dish edge to carry out careful liquid suction and injection operation, which undoubtedly increases the complexity and workload of experiment operation, and puts forward higher requirements to the operation skill and patience of the experiment personnel.

[0004] Therefore, the utility model provides a mouse small intestine epithelial cell culture appliance to solve the above problems. UTILITY MODEL CONTENT

[0005] In view of the deficiency of prior art, the utility model provides a mouse small intestine epithelial cell culture appliance, solves the above problems.

[0006] In order to achieve the above object, the utility model discloses a mouse small intestinal epithelial cell culture appliance through the following technical schemes, a culture structure includes petri dish, the inside lower surface center position of petri dish is fixedly provided with support, the upper surface of support is fixedly connected with tray, the annular flange of tray upper surface is connected with film through the mode of annular flange bonding, the inside of support is provided with liquid channel, and the inside of support is provided with several liquid holes that are communicated with the inside of petri dish along liquid channel, the inside sliding seal of liquid channel is provided with piston, the upper end of piston is fixed with film, and the lower end of piston is rotatably connected with screw rod in the inside of liquid channel, the rod body of screw rod is downwardly worn out liquid channel, and the screw thread transmission of silk cover that is integrally arranged with the lower end port position of liquid channel is up and down, the inside of silk cover is provided with liquid exhaust pipeline that is opened downwardly in L type from the position communicated with liquid channel to the lower side of silk cover silk hole, and the lower end port of liquid exhaust pipeline is provided with the interface that is opened in butt joint.

[0007] Preferably, the center of the film is integrally provided with a reinforcing piece for assisting take-off.

[0008] Preferably, the liquid hole is inclined downwardly from the position close to the bottom surface in the inside of the petri dish to the inside of the liquid hole.

[0009] Preferably, the piston is initially located at the port position of the liquid hole to block the lower port of the liquid hole.

[0010] Preferably, the end of the screw rod worn out the silk cover is fixedly provided with a rotating head, and the bottom of the petri dish is integrally provided with a supporting plate.

[0011] Beneficial effects

[0012] The utility model provides a mouse small intestinal epithelial cell culture appliance, which has the following beneficial effects compared with the prior art:

[0013] (1), the mouse small intestinal epithelial cell culture appliance, through the cooperation of support, liquid channel, liquid hole, piston, screw rod, silk cover and liquid exhaust pipeline, when replacing the culture medium, only needs to move the petri dish to the clean bench, connects the liquid exhaust pipeline with the suction equipment, manually rotates the screw rod to move the piston, unblocks the liquid hole, realizes the rapid suction of the culture medium, and then can conveniently inject the new culture medium, which is more simple and direct than the operation process of using the pipette at the edge of the petri dish, reduces the cumbersome steps in the process of replacing the culture medium, avoids the situation that the cells are damaged due to contact with the cells during the suction process, improves the replacement efficiency and the conditions for successful cultivation, and is especially suitable for cell culture experiments that require frequent replacement of the culture medium, saves time and effort for the experimenters.

[0014] (2), the mouse small intestine epithelial cell culture device, when the cell needs to be cleaned, the cleaning liquid device can be connected through the liquid suction pipeline, the cleaning liquid is injected into the bottom of the culture dish and submerged in the cell through the similar operation mode of replacing the culture medium, and then the cleaning liquid is sucked out, in the whole process, the liquid channel, the liquid hole and the like structure ensure that the cleaning liquid can uniformly reach around the cell, the cell and the culture dish surface are fully cleaned, residual culture medium, metabolites and impurities and the like are effectively removed, compared with the traditional culture dish relying on the operation of the pipette at the edge, the design can realize more thorough and efficient cleaning effect, which is helpful to maintain the cleanliness of the cell culture environment, reduce the potential influence of impurities on the cell growth, and ensure the accuracy and reliability of the cell culture experiment. BRIEF DESCRIPTION OF DRAWINGS

[0015] Fig. 1 is a three-dimensional structure of the utility model;

[0016] Fig. 2 is a structure section view of the utility model;

[0017] Fig. 3 is a structure disassembly view of the utility model.

[0018] In the drawing: 1, culture structure;11, culture dish;12, tray;121, film;122, reinforcing sheet;13, support;131, liquid hole;132, liquid channel;14, piston;141, screw;15, wire sleeve;151, liquid suction pipeline;16, supporting plate. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0020] Please refer to Figs. 1-3The utility model provides a kind of mouse small intestinal epithelial cell culture apparatus, including culture structure 1, the culture structure 1 includes culture dish 11, the inside lower surface of the culture dish 11 is fixedly provided with support 13 in the center position, the support 13 is guide wheel, and is fixedly connected with tray 12 on upper surface, the tray 12 is cymbal structure, and the upward surface is provided with specified concave, and the radius of upper surface is slightly smaller than the inner radius of culture dish 11, and is connected and fixed with film 121 by the way of being bonded with the ring flange of film 121 at ring flange of upper surface, the center position of the film 121 is integrally provided with reinforced sheet 122 for assisting take-off, the inside of the support 13 is opened with liquid channel 132 directly below reinforced sheet 122, and the inside of support 13 is opened with several liquid holes 131 for being communicated with the inside of culture dish 11 along liquid channel 132, and liquid hole 131 is opened with the inside of culture dish 11 being inclined downward from the position close to bottom surface to the inside of liquid hole 131, the inside of liquid channel 132 is slidably sealed with piston 14, and initially piston 14 is at the port position of liquid hole 131, carries out the plugging of lower port of liquid hole 131, the upper end of the piston 14 is fixed with reinforced sheet 122, and the lower end of piston 14 is rotatably connected with screw rod 141 in the inside of liquid channel 132, the rod body of screw rod 141 is worn out liquid channel 132, and is threadedly driven up and down with the cooperation of wire sleeve 15 integrally arranged at the lower port position of liquid channel 132, the end of screw rod 141 worn out wire sleeve 15 is fixedly provided with rotating head, and the type and size of rotating head are adjusted according to demand, the inside of wire sleeve 15 is opened with liquid discharge pipeline 151 in L type from the position being communicated with liquid channel 132 to the lower side of wire sleeve 15 silk hole one side downward, and the lower port of liquid discharge pipeline 151 is provided with interface for docking opening, the bottom of the culture dish 11 is integrally provided with supporting tray 16.

[0021] When mouse small intestinal epithelial cell culture medium needs to be replaced, first, culture dish 11 in the incubator is moved to the stable position of sterile clean bench, liquid discharge pipeline 151 connector is connected with suction equipment, then screw rod 141 is manually rotated to move upward, piston 14 is pushed to unseal lower port of liquid hole 131, while reinforced sheet 122 carries cells to expel culture medium on the surface of film 121, and suction equipment sucks culture medium in culture dish 11 into and stores through liquid hole 131, liquid channel 132 and liquid discharge pipeline 151, if cleaning is needed, clean liquid equipment is connected with liquid discharge pipeline 151 interface to inject clean liquid to submerge cells, and then suction equipment is used to suck out, after cleaning, new culture medium equipment is connected to inject culture medium through liquid discharge pipeline 151, liquid channel 132 and liquid hole 131 to submerge tray 12, then screw rod 141 is manually rotated to move downward to reset to seal liquid hole 131, and the remaining culture medium in liquid channel 132 is sucked out, finally, culture dish 11 is placed back in the incubator to complete the replacement of culture medium.

[0022] In summary, through the cooperation of the bracket 13, the liquid channel 132, the liquid hole 131, the piston 14, the screw rod 141, the wire sleeve 15, and the liquid suction pipeline 151, when replacing the culture medium, only need to move the culture dish 11 to the clean bench, connect the liquid suction pipeline 151 with the suction equipment, manually rotate the screw rod 141 to move the piston 14, unseal the liquid hole 131, realize the rapid suction of the culture medium, and then conveniently inject the new culture medium. Compared with the traditional operation of using a pipette at the edge of the culture dish 11, the operation process is more simple and direct, reduces the cumbersome steps in the process of replacing the culture medium, avoids the situation that the cells are damaged by contacting the cells during the suction process, improves the replacement efficiency and the success conditions of cultivation, and is especially suitable for cell culture experiments that need to replace the culture medium frequently. It saves time and effort for the experimenters. In addition, when the cells need to be cleaned, the liquid suction pipeline 151 can be connected with the cleaning liquid equipment, and the cleaning liquid can be injected into the bottom of the culture dish 11 and submerged the cells through the similar operation method as replacing the culture medium, and then the cleaning liquid is sucked out. During the whole process, the liquid channel 132, the liquid hole 131 and other structures ensure that the cleaning liquid can uniformly reach around the cells, and the cells and the surface of the culture dish 11 are fully cleaned, effectively removing the residual culture medium, metabolic products, impurities and the like. Compared with the traditional culture dish 11 relying on the pipette at the edge, this design can achieve more thorough and efficient cleaning effect, which helps to maintain the cleanliness of the cell culture environment, reduces the potential impact of impurities on cell growth, and ensures the accuracy and reliability of the cell culture experiment.

[0023] Meanwhile, the contents not described in detail in the specification all belong to the prior art known to those skilled in the art.

[0024] Working principle: when working, first, according to the standard operation steps, the culture medium and mouse small intestinal epithelial cells are placed in the inside of the culture dish 11 and the film 121, and then placed in the incubator for cultivation, during the cultivation process, the cell growth state is observed under the microscope regularly, according to the cell growth and the color change of the culture medium, such as yellowing, indicating that the nutrient composition is consumed or the pH changes, the culture medium is replaced in time, generally, it needs to be replaced once in two to three days, when the culture medium needs to be replaced, first, the culture dish 11 in the incubator is moved to a sterile clean workbench and placed in a stable position in the clean bench, then the joint of the suction pipeline 151 is connected with the suction equipment, after connection, the screw rod 141 is manually rotated, so that the screw rod 141 moves upward along the silk sleeve 15, when the screw rod 141 moves upward, the piston 14 will be pushed to move upward along the liquid channel 132, the upward moving piston 14 will unblock the lower port of the liquid hole 131, at the same time, the reinforcing sheet 122 is slowly lifted from the inside of the tray 12 with the mouse small intestinal epithelial cells, during the slow upward movement of the reinforcing sheet 122, the culture medium on the surface of the film 121 is squeezed out, reducing the retention of the culture medium on the surface of the film 121, at the same time, the suction equipment slowly sucks the culture medium in the culture dish 11, the culture medium enters the liquid channel 132 through the liquid hole 131, and then enters the inside of the suction pipeline 151, and finally is sucked into the inside of the suction equipment through the interface of the suction pipeline 151 and is stored separately, after suction, if the cells need to be cleaned, the equipment containing cleaning liquid is connected with the interface of the sterilized suction pipeline 151, then the cleaning liquid is injected into the inside of the suction pipeline 151 at a constant speed, so that the liquid gradually floods the film 121 and the mouse small intestinal epithelial cells on the surface of the film 121 from the bottom of the culture dish 11, then the suction equipment is connected again, and the cleaning liquid is extracted by the same way, so that the cleaning of the culture dish 11 and the cells can be completed, after cleaning, the new culture medium treated with the new culture medium is connected with the interface of the sterilized suction pipeline 151, then the new culture medium is injected into the inside of the culture dish 11 through the suction pipeline 151, the liquid channel 132 and the liquid hole 131 at a constant speed, until the culture medium floods the tray 12, then the screw rod 141 is manually rotated to move downward together with the silk sleeve 15, and is reset to the initial state to block the lower port of the liquid hole 131, then the remaining culture medium in the liquid channel 132 is extracted, and the replacement of the culture medium is completed, and the culture dish 11 is placed in the incubator again.

[0025] It is to be noted that, in the present document, relational terms such as first and second and the like can be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises", "comprising", or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but can include other elements not expressly listed or inherent to such process, method, article, or apparatus.

[0026] While the embodiments of the present application have been illustrated and described, it will be understood by those skilled in the art that various changes, modifications, substitutions, and alterations can be made therein without departing from the spirit and scope of the application, which is defined by the appended claims and their equivalents.

Claims

1. A mouse small intestinal epithelial cell culture device, characterized by, Include: Culture structure (1), the culture structure (1) includes petri dish (11), the lower surface inside the center of petri dish (11) is fixedly provided with support (13), the upper surface of support (13) is fixedly connected with tray (12), the ring flange of tray (12) upper surface is connected with film (121) by the way of being bonded with the ring flange of film (121), the inside of support (13) is provided with liquid channel (132), and the inside of support (13) is provided with several liquid holes (131) along liquid channel (132) ring around, which are communicated with liquid channel (132) and the inside of petri dish (11), the inside of liquid channel (132) is slidably sealed with piston (14), the upper end of piston (14) is fixed with film (121), and the lower end of piston (14) is rotatably connected with screw rod (141) in the inside of liquid channel (132), the rod body of screw rod (141) penetrates out of liquid channel (132) downward, and is threadedly driven up and down with the silk cover (15) integrally arranged at the lower end port position of liquid channel (132), the inside of silk cover (15) is provided with the liquid exhaust pipeline (151) of L type from the position communicated with liquid channel (132) to the lower side of the side of silk cover (15) on one side of silk hole of silk cover (15), and the lower end port of liquid exhaust pipeline (151) is provided with the interface of docking opening.

2. The mouse small intestinal epithelial cell culture device according to claim 1, wherein: The center of film (121) is integrally provided with reinforced sheet (122) for assisting take-off and landing.

3. The mouse small intestinal epithelial cell culture device of claim 1, wherein: The liquid hole (131) is inclined downward from the position close to the bottom surface inside the petri dish (11) to the inside of liquid hole (131).

4. The mouse small intestinal epithelial cell culture device of claim 1, wherein: Initially, the piston (14) is at the port position of liquid hole (131), and the lower end port of liquid hole (131) is blocked.

5. The mouse small intestinal epithelial cell culture device of claim 1, wherein: The end of screw rod (141) penetrating out of silk cover (15) is fixedly provided with rotating head, and the bottom of petri dish (11) is integrally provided with supporting plate (16).