Colorectal cancer micro-tumor cell culture device

Abstract

The utility model discloses a colorectal cancer micro-tumor cell culture device which comprises a step-shaped support, and a culture solution bottle, a cell culture bottle and a waste liquid storage bottle are sequentially arranged on the step-shaped support from top to bottom. A liquid outlet in the bottom of the culture solution bottle is connected with a liquid inlet of the cell culture bottle through a first guide pipe, a liquid outlet in the bottom of the cell culture bottle is connected with a liquid inlet of the waste liquid storage bottle through a second guide pipe, and the liquid outlet in the bottom of the culture solution bottle is higher than the liquid inlet of the cell culture bottle. A liquid outlet in the bottom of the cell culture bottle is higher than a liquid inlet of the waste liquid storage bottle, and a liquid outlet in the bottom of the cell culture bottle is higher than a liquid inlet of the waste liquid storage bottle. Compared with the mode that a suction pump pumps out waste culture liquid in the cell culture bottle, the adherent cells can be effectively prevented from falling off or the cells are not firmly adhered to the wall.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture technical field, concretely is a kind of colorectal cancer microtumor cell culture device. BACKGROUND

[0002] The utility model discloses a kind of colorectal cancer microtumor cell culture devices with the utility model patent with the publication number CN220685160U, including base, the top outer wall of the base is fixedly connected with incubator and operation box, the inner wall of the operation box is provided with culture flask, liquid storage bottle, washing bottle, waste liquid recovery bottle, the inner wall of the liquid storage bottle is inserted with infusion tube, the top of the liquid storage bottle is provided with suction pump one connected with infusion tube, the inner side of the liquid storage bottle is provided with stirring device, the end of infusion tube far from the liquid storage bottle is set in culture flask, one side of the washing bottle is provided with suction pump two, the water inlet end and water outlet end of suction pump two are provided with flush pipe one and flush pipe two respectively, the end of flush pipe one and flush pipe two far from suction pump two is set in washing bottle and culture flask respectively, one side of the waste liquid recovery bottle is provided with suction pump three, the water outlet end and water inlet end of suction pump three are provided with waste liquid recovery pipe one and waste liquid recovery pipe two respectively, the other end of waste liquid recovery pipe one, waste liquid recovery pipe two is set in waste liquid recovery bottle, culture flask respectively;The top of the culture flask is provided with sealing cover, the inner wall top of the operation box is fixedly connected with electric push rod, one end of the electric push rod is connected with sealing cover, the inner wall bottom of the operation box is provided with receiving vessel;The liquid storage bottle includes liquid storage bottle bottle body and liquid storage bottle bottle cap, the stirring device includes stirring motor, the stirring motor is arranged on the outer wall of liquid storage bottle bottle cap, the output shaft of the stirring motor is fixedly connected with stirring rod, the outer wall of the stirring rod is fixedly connected with stirring paddle, and the stirring paddles are arrayed on the outer wall of the stirring rod;The end of flush pipe two set in culture flask is fixedly connected with flush spray head;The outer wall of one side of the incubator is fixedly connected with control box, the main machine is arranged in the control box, and the suction pump one, suction pump two, suction pump three and stirring motor are electrically connected with the main machine;The top outer wall of the incubator is evenly provided with heat dissipation port.

[0003] The above patent has the following deficiencies:

[0004] The old culture solution in the culture flask is extracted by the suction pump. During the working process of the suction pump, suction force is generated in the culture flask. This suction force can cause the adherent cells to fall off or the cells to adhere weakly, thereby affecting the cell culture effect. UTILITY MODEL CONTENTS

[0005] In view of the problems existing in the prior art, the utility model is proposed.

[0006] Therefore, the purpose of the present application is to provide a kind of colorectal cancer microtumor cell culture device, the old culture solution in culture bottle is extracted by using suction pump, suction pump produces suction force in the process of working in culture bottle, this suction force will make that adherent cell has exfoliation, or make that cell adherent is not firm, to affect cell culture effect problem.

[0007] To solve the above technical problems, according to one aspect of the present application, the present application provides the following technical scheme:

[0008] A kind of colorectal cancer microtumor cell culture device, including ladder type support, culture solution bottle, cell culture bottle and waste liquid storage bottle are sequentially arranged from high to bottom on the ladder type support;

[0009] The liquid outlet of the bottom of the culture solution bottle is connected with the liquid inlet of the cell culture bottle by the first conduit, and the liquid outlet of the bottom of the cell culture bottle is connected with the liquid inlet of the waste liquid storage bottle by the second conduit.

[0010] As a preferred scheme of the present application, the height of the liquid outlet of the bottom of the culture solution bottle is higher than the height of the liquid inlet of the cell culture bottle, and the height of the liquid outlet of the bottom of the cell culture bottle is higher than the height of the liquid inlet of the waste liquid storage bottle.

[0011] As a preferred scheme of the present application, the top of the culture solution bottle is provided with a first plugging cover, the top of the cell culture bottle is provided with a second plugging cover, and the top of the waste liquid storage bottle is provided with a third plugging cover.

[0012] As a preferred scheme of the present application, the bottom of the waste liquid storage bottle is connected with a drainage pipe, and valves are installed on the liquid outlet of the bottom of the culture solution bottle, the liquid outlet of the bottom of the cell culture bottle and the drainage port of the bottom of the waste liquid storage bottle.

[0013] As a preferred scheme of the present application, the first limiting ring, the second limiting ring and the third limiting ring are sequentially welded on the ladder type support from high to bottom, the bottoms of the culture solution bottle, the cell culture bottle and the waste liquid storage bottle are sequentially inserted into the first limiting ring, the second limiting ring and the third limiting ring, and the ladder type support is fixedly installed in the inner cavity of the first box body.

[0014] As a preferred scheme of the present application, the stirring mechanism is further installed on the first box body.

[0015] The stirring mechanism comprises an L-shaped support welded on the first box body, a motor fixedly installed on the L-shaped support, a stirring shaft connected with an output shaft of the motor through a shaft coupling, and a stirring rod installed on the stirring shaft.

[0016] Compared with the prior art, the present application has the advantages of:

[0017] By setting the culture solution bottle, the cell culture bottle and the waste liquid storage bottle with height difference, the culture solution in the culture solution bottle is injected into the cell culture bottle in sequence by gravity, which can effectively avoid the detachment of adherent cells or make the adherent cells not firmly adhere. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 The structure schematic view provided for the embodiment 1 of the present application is shown in the figure;

[0019] Figure 2 The structure schematic view provided for the embodiment 1 of the present application is shown in the figure; Figure 1 The sectional view of the culture solution bottle provided for the embodiment 2 of the present application is shown in the figure;

[0020] Figure 3 The sectional view of the culture solution bottle provided for the embodiment 2 of the present application is shown in the figure; Figure 1 The sectional view of the culture solution bottle provided for the embodiment 2 of the present application is shown in the figure;

[0021] Figure 4 The sectional view of the culture solution bottle provided for the embodiment 2 of the present application is shown in the figure.

[0022] In the figure: base 1, second box body 2, second door plate 3, first box body 4, first door plate 5, first liquid injection pipe 6, liquid discharge pipe 7, culture solution bottle 8, first plugging cover 81, cell culture bottle 9, second plugging cover 91, waste liquid storage bottle 10, third plugging cover 101, stepped support 11, first limiting ring 12, third limiting ring 13, motor 14, L-shaped support 15, second liquid injection pipe 16, stirring shaft 17, stirring rod 18, mounting ring 181, second limiting ring 19. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical scheme and advantages of the present application more clear, the embodiments of the present application will be further described in detail below with reference to the drawings.

[0024] Embodiment 1:

[0025] The present application provides a colorectal cancer microtumor cell culture device, please refer to Figures 1-2, including a stepped support 11 fixedly installed in the inner cavity of the first box body 4, that is, the stepped support 11 is welded on the inner wall of the first box body 4, and the left side of the first box body 4 is provided with a second box body 2, the bottom end of the second box body 2 and the first box body 4 are fixedly connected with a base 1, the opening of the second box body 2 is connected with a second door plate 3 through a hinge, and the opening of the first box body 4 is connected with a first door plate 5 through a hinge; the stepped support 11 is sequentially provided with a culture solution bottle 8, a cell culture bottle 9 and a waste liquid storage bottle 10 from high to low;

[0026] The liquid outlet at the bottom of the culture solution bottle 8 is connected with the liquid inlet of the cell culture bottle 9 through a first pipeline, and the liquid outlet at the bottom of the cell culture bottle 9 is connected with the liquid inlet of the waste liquid storage bottle 10 through a second pipeline.

[0027] The height of the liquid outlet at the bottom of the culture solution bottle 8 is higher than the height of the liquid inlet of the cell culture bottle 9, and the height of the liquid outlet at the bottom of the cell culture bottle 9 is higher than the height of the liquid inlet of the waste liquid storage bottle 10.

[0028] The top end of the culture solution bottle 8 is provided with a first plugging cover 81, a through hole is formed in the first plugging cover 81, and a first liquid injection pipe 6 is inserted into the through hole, so that the culture solution can be injected into the culture solution bottle 8 through the first liquid injection pipe 6; the top end of the cell culture bottle 9 is provided with a second plugging cover 91, and the top end of the waste liquid storage bottle 10 is provided with a third plugging cover 101.

[0029] A liquid discharge pipe 7 is connected with the liquid discharge port at the bottom of the waste liquid storage bottle 10, and valves are installed on the liquid outlet at the bottom of the culture solution bottle 8, the liquid outlet at the bottom of the cell culture bottle 9 and the liquid discharge port at the bottom of the waste liquid storage bottle 10.

[0030] The first limiting ring 12, the second limiting ring 19 and the third limiting ring 13 are sequentially welded on the stepped support 11 from high to low, and the bottoms of the culture solution bottle 8, the cell culture bottle 9 and the waste liquid storage bottle 10 are sequentially inserted into the first limiting ring 12, the second limiting ring 19 and the third limiting ring 13.

[0031] In specific use, the valve on the liquid outlet at the bottom of the culture solution bottle 8 is opened, and the culture solution in the culture solution bottle 8 enters the cell culture bottle 9;

[0032] When the old culture solution in the cell culture bottle 9 is replaced, the valve on the liquid outlet at the bottom of the cell culture bottle 9 is opened, so that the old culture solution in the cell culture bottle 9 flows into the waste liquid storage bottle 10, and the valve on the liquid outlet at the bottom of the waste liquid storage bottle 10 is opened, so that the waste culture solution in the waste liquid storage bottle 10 can be discharged.

[0033] Example 2:

[0034] Referring to the accompanying drawings Figures 3-4 Different from example 1, it further comprises a stirring mechanism installed on the first box body 4.

[0035] The stirring mechanism comprises an L-shaped support 15 welded on the first box body 4, a motor 14 fixedly installed on the L-shaped support 15, an output shaft of the motor 14 connected with a stirring shaft 17 through a shaft coupling, and a stirring rod 18 installed on the stirring shaft 17, wherein the stirring rod 18 has an installation ring 181 at the middle portion, and the installation ring 181 is sleeved on the stirring shaft 17 and fixed thereon through bolts.

[0036] The right end of the culture solution bottle 8 is connected with a second liquid injection pipe 16, and the top end of the second liquid injection pipe 16 penetrates through the first box body 4 and extends outside the first box body 4.

[0037] In specific use, the valve on the liquid outlet at the bottom of the culture solution bottle 8 is opened, and the culture solution in the culture solution bottle 8 flows into the cell culture bottle 9.

[0038] When the old culture solution in the cell culture bottle 9 is replaced, the valve on the liquid outlet at the bottom of the cell culture bottle 9 is opened, and the old culture solution in the cell culture bottle 9 flows into the waste liquid storage bottle 10, and the valve on the liquid outlet at the bottom of the waste liquid storage bottle 10 is opened, so that the waste culture solution in the waste liquid storage bottle 10 is discharged.

[0039] The motor 14 is started to drive the stirring shaft 17 and the stirring rod 18 to rotate, so as to stir the culture solution in the culture solution bottle 8, thereby making the culture solution more uniform.

[0040] Although the present application has been described with reference to the embodiments above in the foregoing description, it is to be understood that various modifications can be made without departing from the scope of the present application. More particularly, the features of the disclosed embodiments can be combined in any manner, and the disclosure of the present application is intended to cover all such modifications and equivalents. Therefore, the present application is not limited to the specific embodiments disclosed in the specification, but encompasses all the technical solutions falling within the scope of the claims.

Claims

1. A colorectal cancer microtumor cell culture device comprising a stepped scaffold (11), characterized in that: The step-type support (11) is sequentially provided with a culture solution bottle (8), a cell culture bottle (9) and a waste liquid storage bottle (10) from top to bottom. The liquid outlet at the bottom of the culture solution bottle (8) is connected to the liquid inlet of the cell culture bottle (9) through a first conduit, and the liquid outlet at the bottom of the cell culture bottle (9) is connected to the liquid inlet of the waste liquid storage bottle (10) through a second conduit.

2. The colorectal cancer micrometastasis cell culture device of claim 1, wherein, The height of the liquid outlet at the bottom of the culture solution bottle (8) is higher than the height of the liquid inlet of the cell culture bottle (9), and the height of the liquid outlet at the bottom of the cell culture bottle (9) is higher than the height of the liquid inlet of the waste liquid storage bottle (10).

3. The colorectal cancer micrometastasis cell culture device of claim 2, wherein, The top of the culture solution bottle (8) is capped with a first sealing cover (81), the top of the cell culture bottle (9) is capped with a second sealing cover (91), and the top of the waste liquid storage bottle (10) is capped with a third sealing cover (101).

4. The colorectal cancer micrometastasis cell culture device of claim 3, wherein, A liquid discharge pipe (7) is connected to the liquid discharge port at the bottom of the waste liquid storage bottle (10), and a valve is installed on each of the liquid outlet at the bottom of the culture solution bottle (8), the liquid outlet at the bottom of the cell culture bottle (9) and the liquid discharge port at the bottom of the waste liquid storage bottle (10).

5. The device for culturing micro-tumor cells of colorectal cancer according to any one of claims 1-4, characterized in that, The step-type support (11) is sequentially welded with a first limiting ring (12), a second limiting ring (19) and a third limiting ring (13) from top to bottom, the bottoms of the culture solution bottle (8), the cell culture bottle (9) and the waste liquid storage bottle (10) are sequentially inserted into the first limiting ring (12), the second limiting ring (19) and the third limiting ring (13), and the step-type support (11) is fixedly installed in the inner cavity of the first box body (4).

6. The colorectal cancer micrometastasis cell culture device of claim 1, wherein, The stirring mechanism is installed on the first box body (4). The stirring mechanism includes an L-shaped support (15) welded on the first box body (4), a motor (14) fixedly installed on the L-shaped support (15), a stirring shaft (17) connected to the output shaft of the motor (14) through a coupling, and a stirring rod (18) installed on the stirring shaft (17).

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