Three-dimensional bioreactor
By combining pneumatic components with support rods and connecting blocks, the problems of sealing and ease of replacement of the culture platform in the three-dimensional bioreactor are solved, enabling efficient cell culture medium replacement and improving experimental efficiency and result accuracy.
Patent Information
- Application Number
- CN202423250661.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-27
- Publication Date
- 2025-12-26
- Estimated Expiration
- 2034-12-27
AI Technical Summary
Existing three-dimensional bioreactors have shortcomings in terms of sealing and ease of replacement of culture platforms, which affect the quality and efficiency of cell culture. Furthermore, the process of changing the culture medium is complicated and increases the risk of contamination.
The design employs pneumatic components in conjunction with support rods and connecting blocks to facilitate the installation and replacement of the terraced cultivation platform. Inlet and outlet valves are installed on the housing to ensure sealing and efficient replacement of the culture medium.
It simplifies experimental procedures, reduces the risk of contamination, and improves experimental efficiency and accuracy of results, making it suitable for cell culture in fields such as biomedicine, tissue engineering, and cell therapy.
Smart Images
Figure CN223723143U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the technical field of bioreactor, especially to a three-dimensional bioreactor. BACKGROUND
[0002] In the field of biotechnology, three-dimensional bioreactors are widely used in biological medicine, tissue engineering, cell therapy and other research directions as an advanced cell culture equipment. This kind of reactor can effectively promote the proliferation, differentiation and functional expression of cells by simulating the three-dimensional growth environment of cells in vivo, thereby accelerating the transformation and application of research results. However, the existing three-dimensional bioreactor still has some significant deficiencies in design, which limits the optimization of its performance and the expansion of its application range.
[0003] Traditional three-dimensional bioreactors often have defects in sealing design, which may cause external pollutants (such as microorganisms, dust, etc.) to enter the reactor interior through the gap during cell culture, seriously affecting the growth environment and culture quality of cells. At the same time, poor sealing may also cause evaporation of the culture solution, changing the composition ratio of the culture solution and further affecting the results of cell culture.
[0004] Many existing three-dimensional bioreactors adopt a fixed culture platform in design, which cannot be replaced or adjusted once the culture starts. This not only limits the demand for culture platform material, shape or size under different experimental conditions, but also increases the complexity and cost of experimental operation. Especially in long-term culture or experiments that require frequent replacement of culture conditions, the limitations of this design are particularly prominent.
[0005] Culture medium replacement is an important link in the process of cell culture, but the existing three-dimensional bioreactor is not convenient in this operation. Some reactors need to be disassembled to complete the replacement of the culture medium, which not only increases the operation difficulty and time cost, but also may cause additional physical damage to cells. In addition, the risk of contamination during the replacement of the culture medium is also a problem that cannot be ignored.
[0006] The contact area of the culture platform directly determines the number of cells that can be accommodated and the degree of interaction between cells, which has an important influence on cell culture effect. However, many existing three-dimensional bioreactors are too compact in design, resulting in limited contact area of the culture platform, which cannot meet the experimental demand of large-scale cell culture or high cell density. This not only limits the scale and efficiency of cell culture, but also affects the accuracy and reliability of experimental results. SUMMARY
[0007] In view of the above, in order to overcome the defects of the prior art, the utility model provides a three -dimensional bioreactor, including the box, the top rotationally connected with the apron of box, the bottom of apron is equipped with support rod, the bottom of support rod is equipped with pneumatic assembly, the outer edge of apron is equipped with sealing strip, the bottom of pneumatic assembly is equipped with connecting block, the top of connecting block is equipped with terrace culture platform, the top of apron is equipped with sealing assembly, one side of box is equipped with liquid inlet valve, the other side of box is equipped with liquid outlet valve.
[0008] Preferably, the pneumatic assembly includes a frame, the inside of the frame is slidably connected with the support rod, the bottom of the frame is provided with a first piston rod, the outside of the first piston rod is slidably connected with a first cylinder, and the first cylinder is connected with the box.
[0009] Preferably, the bottom of the first piston rod is provided with a spring, and the bottom of the first cylinder is provided with two connecting pipes which are symmetrically arranged left and right.
[0010] Preferably, the rear side of the connecting pipe is provided with a second cylinder, the inside of the second cylinder is provided with a second piston rod, and the inside of the second piston rod is slidably connected with the connecting block.
[0011] Preferably, the sealing assembly includes a first block, the first block is fixedly connected with the apron, the bottom of the first block is provided with a second block, the inside of the second block is fixedly connected with the box, and the inside of the first block and the second block is penetratedly connected with a screw.
[0012] Compared with the prior art, the utility model has the beneficial effects that:
[0013] Through the cooperation of the pneumatic assembly, the support rod and the connecting block, the terrace culture platform is conveniently installed and replaced. This design not only simplifies the experimental operation and reduces the pollution risk in the cell culture process, but also improves the experimental efficiency. When the culture platform needs to be replaced, it can be easily completed by adjusting the pneumatic assembly without disassembling the entire reactor, thereby greatly saving time and effort. The liquid inlet valve and the liquid outlet valve are respectively arranged on the box, so that the replacement process of the culture liquid is more convenient and efficient. This design not only reduces the pollution risk in the culture liquid replacement process, but also ensures the stability and consistency of the cell culture environment, which is conducive to obtaining more accurate experimental results. The three-dimensional bioreactor of the utility model is not only suitable for research fields such as biological medicine, tissue engineering and cell therapy, but also can be widely applied to other scientific experiments that need to simulate the three-dimensional growth environment of cells. Its convenient operation, efficient performance and good sealing performance make the reactor have broad market prospect and application value. BRIEF DESCRIPTION OF DRAWINGS
[0014] Figure 1 It is a whole structure schematic view of the utility model.
[0015] Figure 2 It is a closed state schematic view of the utility model.
[0016] Figure 3 It is a pneumatic assembly structure schematic view of the utility model.
[0017] Figure 4 It is a connecting block structure schematic view of the utility model.
[0018] Marked number in the drawing: 1, cover plate;2, sealing strip;3, first square;4, box body;5, second square;6, terrace culture platform;7, liquid outlet valve;8, liquid inlet valve;9, screw;10, support rod;11, frame;12, first piston rod;13, first cylinder;14, spring;15, connecting pipe;16, second cylinder;17, second piston rod;18, connecting block. DETAILED DESCRIPTION
[0019] The following will be combined with the accompanying Figures 1-4 The specific embodiment of the utility model is further explained in detail.
[0020] The utility model includes box body 4, the top of box body 4 is rotatably connected with cover plate 1, and the rotatable connection mode makes cover plate 1 can be conveniently opened or closed, and the operation of cell culture is convenient, the bottom of cover plate 1 is equipped with support rod 10, the bottom of support rod 10 is equipped with pneumatic assembly, and support rod 10 is used to connect pneumatic assembly, the outer edge of cover plate 1 is equipped with sealing strip 2, sealing strip 2 is made of soft and durable material, can effectively prevent the pollutant of outside from entering reactor interior, and guarantee the air tightness of box body 4, the bottom of pneumatic assembly is equipped with connecting block 18, the top of connecting block 18 is equipped with terrace culture platform 6, and pneumatic assembly can cooperate with connecting block 18, and the installation and replacement of terrace culture platform 6 are convenient, the top of cover plate 1 is equipped with sealing assembly, realizes the sealing between cover plate 1 and box body 4, one side of box body 4 is equipped with liquid inlet valve 8, the other side of box body 4 is equipped with liquid outlet valve 7, liquid inlet valve 8 is used to inject new culture solution into reactor, and liquid outlet valve 7 is used to discharge old culture solution.This design makes the replacement process of culture solution more convenient and efficient, and the whole adopts the material quality of engineering plastics, is light and durable.
[0021] The pneumatic assembly comprises a frame body 11, the inside of the frame body 11 is in sliding connection with the supporting rod 10, the supporting rod 10 is driven to rotate by the cover plate 1, the frame body 11 in sliding connection is driven to vertically ascend and descend by the supporting rod 10, the frame body 11 is driven to descend by the cover plate 1 being closed, the bottom of the frame body 11 is provided with a first piston rod 12, the outside of the first piston rod 12 is in sliding connection with a first cylinder body 13, the first cylinder body 13 is connected with the box body 4, the first piston rod 12 pushes the gas in the first cylinder body 13 out, the bottom of the first piston rod 12 is provided with a spring 14, the bottom of the first cylinder body 13 is provided with two connecting pipes 15, the two connecting pipes 15 are symmetrically arranged, the rear side of the connecting pipe 15 is provided with a second cylinder body 16, the high-pressure gas enters the second cylinder body 16, the inside of the second cylinder body 16 is provided with a second piston rod 17, the inside of the second piston rod 17 is in sliding connection with a connecting block 18, the gas pushes the second piston rod 17 to move inwards and be clamped with the connecting block 18, so that the fixing of the terrace culture platform 6 is completed, and vice versa.
[0022] The sealing assembly comprises a first block 3, the first block 3 is fixedly connected with the cover plate 1, the bottom of the first block 3 is provided with a second block 5, the inside of the second block 5 is fixedly connected with the box body 4, the inside of the first block 3 and the second block 5 is penetrated and connected with a screw 9, by rotating the screw 9, the first block 3 and the second block 5 can be tightly attached, so that the sealing between the cover plate 1 and the box body 4 is realized.
[0023] When the utility model is used, through the cooperation of the pneumatic assembly, the supporting rod 10 and the connecting block 18, the convenient installation and replacement of the terrace culture platform 6 are realized. This design not only simplifies the experimental operation and reduces the pollution risk in the cell culture process, but also improves the experimental efficiency. When the culture platform needs to be replaced, it can be easily completed by adjusting the pneumatic assembly, without disassembling the entire reactor, so that time and energy are greatly saved. The liquid inlet valve 8 and the liquid outlet valve 7 are arranged on the box body 4 respectively, so that the replacement process of the culture liquid is more convenient and efficient. This design not only reduces the pollution risk in the culture liquid replacement process, but also guarantees the stability and consistency of the cell culture environment, which is beneficial to obtain more accurate experimental results. The three-dimensional bioreactor of the utility model is not only suitable for biological medicine, tissue engineering, cell therapy and other research fields, but also can be widely applied to other scientific experiments that need to simulate the three-dimensional growth environment of cells. The convenient operation, efficient performance and good sealing performance make the reactor have broad market prospect and application value.
[0024] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and is not intended to limit the present application, although the foregoing embodiments of the present application has been described in detail, for the skilled in the art, it still can be modified, or for part of the technical features of the equivalent replacement, the spirit and principles of the present application, made any modification, equivalent replacement, improvement, etc., should be included within the scope of the present application.
Claims
1. A three-dimensional bioreactor comprising a box (4), characterized in that, The top of the box (4) is rotatably connected with a cover plate (1), the bottom of the cover plate (1) is provided with a supporting rod (10), the bottom of the supporting rod (10) is provided with a pneumatic assembly, the outer edge of the cover plate (1) is provided with a sealing strip (2), the bottom of the pneumatic assembly is provided with a connecting block (18), the top of the connecting block (18) is provided with a terrace culture platform (6), the top of the cover plate (1) is provided with a sealing assembly, one side of the box (4) is provided with a liquid inlet valve (8), the other side of the box (4) is provided with a liquid outlet valve (7).
2. The three-dimensional bioreactor of claim 1, wherein, The pneumatic assembly comprises a frame (11), the inside of the frame (11) is slidably connected with the supporting rod (10), the bottom of the frame (11) is provided with a first piston rod (12), the outer side of the first piston rod (12) is slidably connected with a first cylinder (13), and the first cylinder (13) is connected with the box (4).
3. A three-dimensional bioreactor according to claim 2, wherein The bottom of the first piston rod (12) is provided with a spring (14), the bottom of the first cylinder (13) is provided with two connecting pipes (15), and the two connecting pipes (15) are symmetrically arranged.
4. The three-dimensional bioreactor of claim 3, wherein, The rear side of the connecting pipe (15) is provided with a second cylinder (16), the inside of the second cylinder (16) is provided with a second piston rod (17), and the inner side of the second piston rod (17) is slidably connected with the connecting block (18).
5. The three-dimensional bioreactor of claim 1, wherein, The sealing assembly comprises a first block (3), the first block (3) is fixedly connected with the cover plate (1), the bottom of the first block (3) is provided with a second block (5), the inner side of the second block (5) is fixedly connected with the box (4), and the inside of the first block (3) and the second block (5) penetrates a screw (9).