Olfactory experiment behavior box

Abstract

The utility model relates to an olfactory experiment behavior box, which provides a box body, and an animal fixing device, an air supply device, a water supply device and an imaging device which are arranged in the box body, when an animal is fixed on the animal fixing device, the moving area of the head of the animal is an experiment area, and an air nozzle of the air supply device and a water nozzle of the water supply device are arranged towards the experiment area. The imaging device is used for collecting an image of an experiment area, the water supply device comprises a water nozzle and an adjusting base, the adjusting base comprises a first guide part, a mounting part and a driving motor, the first guide part is arranged on the box body, the mounting part is slidably arranged on the first guide part, and the driving motor is arranged on the box body and used for driving the mounting part to move along the first guide part; the water nozzle is arranged on the mounting piece. According to the device, the driving motor drives the mounting piece provided with the water nozzle to move to different positions, so that the position of the water nozzle is adjusted to meet the requirements of mice of different body types.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of scientific research equipment, in particular to an olfactory experiment behavior box. BACKGROUND

[0002] The olfactory behavior training system is a commonly used scientific research equipment, which includes an olfactory experiment behavior box. The experimental principle is generally to fix a mouse in the olfactory experiment behavior box, then deliver some odor to the mouse, and reward or punish the mouse according to its reaction to the odor. However, the water nozzle of the water supply device in the current olfactory experiment behavior box often supplies water at a fixed position, which is difficult to adapt to mice of different sizes. SUMMARY

[0003] In order to solve the above problems, the present application provides an olfactory experiment behavior box which can automatically adjust the position of the water nozzle to adapt to mice of different sizes.

[0004] The present application provides a box body and an animal fixing device, a gas supply device, a water supply device and an imaging device arranged in the box body. When the animal is fixed in the animal fixing device, the moving area of its head is the experimental area. The gas nozzle of the gas supply device and the water nozzle of the water supply device are arranged towards the experimental area. The imaging device is used to collect images of the experimental area. The water supply device includes a water nozzle and an adjusting base. The adjusting base includes a first guide, a mounting piece and a driving motor. The first guide is arranged in the box body. The mounting piece is slidably arranged in the first guide. The driving motor is arranged in the box body and is used to drive the mounting piece to move along the first guide. The water nozzle is arranged in the mounting piece.

[0005] In the present application, the driving motor drives the mounting piece with the water nozzle to move to different positions to adjust the position of the water nozzle to meet the needs of mice of different sizes. If the position of the water nozzle is adjusted manually by artificial, the box body needs to be opened frequently, which may stimulate the mouse and affect the experimental results. Using the driving motor to automatically adjust the position of the water nozzle can reduce the above influence and also reduce the workload of the experimental personnel. BRIEF DESCRIPTION OF DRAWINGS

[0006] Figure 1 It is an exploded structural schematic view of the internal parts of the olfactory experiment behavior box according to one or more embodiments;

[0007] Figure 2 It is a top view of Figure 1

[0008] Figure 3 It is an exploded structural schematic view of the water supply device;

[0009] Figure 4 It is a top view of the water supply device,

[0010] Figure 5 ​A schematic diagram illustrating the assembly principle of the mounting block assembly and the first guide component;

[0011] Figure 6 This is a schematic diagram of the assembly structure of the first mounting block and the second mounting block;

[0012] Figure 7 This is a schematic diagram illustrating the assembly principle of the first mounting block and the second mounting block.

[0013] Figure 8 A schematic diagram showing the camera assembly in a certain pose;

[0014] Figure 9 A schematic diagram showing the camera assembly in another orientation;

[0015] Figure 10 This is a schematic diagram of the gas supply device.

[0016] Figure 11 Assembly schematic diagram for securing the gripper and animal compartment;

[0017] Figure 12 This is a structural diagram of the box.

[0018] Explanation of reference numerals in the attached figures:

[0019] Olfactory Experiment Behavior Box 1

[0020] Enclosure 10, Main body 11, Door 12, Outer enclosure 13, Sound-absorbing layer 14, Inner enclosure 15

[0021] Animal restraint device 20, animal compartment 21, connecting plate 211, adjustment hole 212, clamp 22, crossbar 221, locking assembly 222, sixth locking screw 223, locking buckle 224, upper locking plate 225, lower locking plate 226, through hole 227.

[0022] Water supply device 30, water nozzle 300, adjusting base 31, first guide 310, mounting component 311, drive motor 312, slider 313, second guide 314, mounting block assembly 315, first screw hole 316, first locking screw 317, first groove 318, first mounting block 319, second mounting block 320, second locking screw 321, second groove 322, first elongated hole 323.

[0023] Air supply device 40, air nozzle 41, air nozzle bracket 42, guide block 421, mounting base plate 422, column 423, air nozzle fixing rod 424, guide groove 425, mounting hole 426, adjustable gap 427, fifth locking screw 428.

[0024] Imaging device 50, camera bracket 51, arc-shaped hole 511, second elongated hole 512, camera assembly 52, third locking screw 53, fourth locking screw 54. Detailed Implementation

[0025] The present application will be further described below with reference to the accompanying drawings and some embodiments. The following embodiments are mainly used to illustrate the technical solutions of the present application and therefore should not be construed as limiting the scope of protection of the present application.

[0026] Unless otherwise defined, the technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains; the terms used herein are primarily for the purpose of describing particular embodiments and are not intended to limit the application; the terms “comprising,” “having,” “including,” and other synonyms having the same or similar meanings in the specification, claims, and foregoing description of the drawings are intended to cover non-exclusive inclusion.

[0027] In the description of the embodiments of this application, technical terms such as "first" and "second" are mainly used to distinguish different objects and should not be construed as indicating relative importance or implicitly specifying the number, specific order, or primary and secondary relationship of the indicated technical features. In the description of the embodiments of this application, "multiple" means two or more, unless otherwise explicitly defined.

[0028] In this document, any particular feature, structure, or characteristic described in any embodiment may be included in at least one embodiment or a combination of at least two embodiments of this application. Those skilled in the art will understand that the embodiments described herein may be combined with other embodiments herein or other embodiments outside of this document.

[0029] In the description of the embodiments of this application, technical terms used to indicate orientation or positional relationships, such as "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., are mainly used to facilitate the description of the embodiments of this application and to simplify the description, rather than being considered as requiring the device or element to have a specific orientation, or to be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0030] In the various embodiments of this application, unless otherwise expressly specified and limited, the technical terms "setting," "installing," "assembling," "connecting," "linking," and "fixing," etc., should be interpreted broadly. Taking connection as an example, it may include fixed connection, detachable connection, or integral molding; it may also include at least one of mechanical connection and electrical connection; it may include direct connection or indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of this application according to the specific circumstances.

[0031] This application provides an olfactory experimental behavior box 1, which is part of an olfactory behavior training system. It mainly includes a box body 10 and an animal restraint device 20, an air supply device 40, a water supply device 30, and an imaging device 50 installed in the box body 10 (see [link to relevant documentation]). Figure 1 and Figure 2 When the animal is fixed in the animal restraint device 20, its head faces the air supply device 40, the water supply device 30, and the imaging device 50. The area where its head moves is the experimental area. The air nozzle 41 of the air supply device 40 and the water nozzle 300 of the water supply device 30 are positioned facing the experimental area. The imaging device 50 is used to acquire images of the experimental area. The air supply device 40 is mainly used to provide mice with odorous gases. If the mouse can accurately identify the desired gas, it can be rewarded with water through the water supply device 30.

[0032] See Figure 1 , Figure 2 , Figure 3 and Figure 4 The water supply device 30 includes a water nozzle 300 and an adjusting base 31. The adjusting base 31 includes a first guide member 310, a mounting member 311, and a drive motor 312. The first guide member 310 is disposed on the housing 10, the mounting member 311 is slidably disposed on the first guide member 310, and the drive motor 312 is disposed on the housing 10 for driving the mounting member 311 to move along the first guide member 310. The water nozzle 300 is disposed on the mounting member 311. The extension direction of the first guide member 310 can be left or right, thereby adjusting the distance between the water nozzle 300 and the animal fixing device 20. The first guide member 310 can be a guide rail, a lead screw, a slide, or other structures, and no specific limitation is made here.

[0033] In this application, the drive motor 312 can drive the mounting piece 311, on which the water nozzle 300 is installed, to move to different positions to adjust the position of the water nozzle 300 to meet the needs of mice of different sizes. If the position of the water nozzle 300 is adjusted manually, the box 10 needs to be opened frequently, which may stimulate the mice and affect the experimental results. Using the drive motor 312 to automatically adjust the position of the water nozzle 300 can reduce the above-mentioned impact and also reduce the workload of the experimenters.

[0034] SeeFigure 3 and Figure 4 The mounting component 311 includes a slider 313, a second guide 314, and a mounting block assembly 315. The slider 313 is slidably disposed on the first guide 310, and the second guide 314 is fixedly disposed on the slider 313, with its extension direction perpendicular to the first guide 310. For example, the extension direction of the second guide 314 can be vertical. The mounting block assembly 315 is slidably disposed on the second guide 314. The mounting block assembly 315 includes a first screw hole 316 and a first locking screw 317. The first locking screw 317 is disposed in the first screw hole 316 and, when it abuts against the second guide 314, restricts the relative movement between the mounting block assembly 315 and the second guide 314.

[0035] See Figure 4 and Figure 5 When the first locking screw 317 abuts against the second guide member 314, the static friction between the mounting block assembly 315 and the second guide member 314 increases, thereby restricting the relative movement of the mounting block assembly 315 and the second guide member 314. When the first locking screw 317 is loosened, that is, after the first locking screw 317 and the second guide member 314 are separated, the mounting block assembly 315 can move along the second guide member 314, thereby adjusting the position of the water nozzle 300. Combined with the drive motor 312 and the second guide member 314, the position of the water nozzle 300 can be adjusted in two directions.

[0036] See Figure 5 The second guide 314 has a first groove 318, the extension direction of the first groove 318 is parallel to the extension direction of the second guide 314, the projection of the first screw hole 316 falls into the first groove 318, and the first locking screw 317 is configured to abut against the first groove 318 to restrict the relative movement between the mounting block assembly 315 and the second guide 314.

[0037] See Figure 3 , Figure 4 , Figure 6 and Figure 7The mounting block assembly 315 includes a first mounting block 319, a second mounting block 320, and a second locking screw 321. The first mounting block 319 is disposed on the second guide member 314 and has a second groove 322. The extension directions of the second groove 322, the second guide member 314, and the first guide member 310 are perpendicular to each other. For example, the extension direction of the second groove 322 can be a front-back direction (in the horizontal plane and perpendicular to the left-right direction). The second mounting block 320 is disposed within the second groove 322 and has a first elongated hole 323, the length direction of which is parallel to the extension direction of the second groove 322. The second locking screw 321 passes through the first elongated hole 323 and is threadedly connected to the first mounting block 319, so that the second mounting block 320 can be fixed to the first mounting block 319.

[0038] In this application, an elongated hole refers to a hole whose length is greater than its width. Its cross-section can be oblong (oblong hole), rectangular, or rounded rectangular; no specific limitation is made here. The second locking screw 321 passes through the first elongated hole 323 and is threadedly connected to the first mounting block 319. When the second locking screw 321 is in the locked state, the second mounting block 320 can be tightly locked onto the first mounting block 319, thereby fixing the second mounting block 320. When the user needs to adjust the front-to-back direction of the water nozzle, the second locking screw 321 can be temporarily unlocked, and then the second mounting block 320 can be moved within the second groove 322 and / or the first elongated hole 323 to adjust its position. After adjustment, the second locking screw 321 is tightened again. Combined with the drive motor 312, the second guide 314, and the second groove 322, the position of the water nozzle 300 can be adjusted in three directions.

[0039] See Figure 1 , Figure 8 and Figure 9 The imaging device 50 may include a camera bracket 51, a camera assembly 52, and a third locking screw 53. The camera bracket 51 includes an arc-shaped hole 511 configured to be positioned about a preset axis (not shown), the preset axis being perpendicular to the spacing between the animal restraint device 20 and the imaging device 50. The third locking screw 53 passes through the arc-shaped hole 511 for threaded connection with the camera assembly 52, and is configured such that: in the locked state, the camera assembly 52 and the camera bracket 51 are fixed together; in the unlocked state, the camera assembly 52 can rotate relative to the camera bracket 51 about the preset axis.

[0040] The third locking screw 53 passes through the arc-shaped hole 511 and is threadedly connected to the camera assembly 52. ​​When the third locking screw 53 is in the locked state, the camera assembly 52 can be firmly locked onto the camera bracket 51. When it is necessary to adjust the angle of the camera assembly 52, the third locking screw 53 can be temporarily unlocked, and then the camera assembly 52 can be rotated. After rotating to the appropriate angle, the third locking screw 53 can be tightened again to fix the camera assembly 52.

[0041] The imaging device 50 includes a camera bracket 51, a camera assembly 52, and a fourth locking screw 54. The camera bracket 51 has two second elongated holes 512, the length of which is parallel to the distance between the animal restraint device 20 and the imaging device 50. The fourth locking screw 54 can be two in number, each located in one of the two second elongated holes 512. The fourth locking screw 54 passes through the second elongated holes 512 and is threadedly connected to the housing 10.

[0042] The fourth locking screw 54 passes through the second elongated hole 512 and is threaded to the housing 10. When the fourth locking screw 54 is in the locked state, the camera bracket 51 can be tightly locked onto the housing 10, thereby restricting the horizontal movement of the camera. After the experimenter unlocks the fourth locking screw 54, the position of the camera bracket 51 can be adjusted, and thus the position of the camera assembly 52 can be adjusted.

[0043] By setting two second elongated holes 512 and two fourth locking screws 54, the experimenter can adjust the position of the camera bracket 51 even if the two fourth locking screws 54 are not completely separated from the threaded holes of the housing 10 during the adjustment process, and the rotation of the camera bracket 51 can be suppressed during the adjustment process.

[0044] See Figure 1 and Figure 10 The air supply device 40 includes an air nozzle 41 and an air nozzle bracket 42. The air nozzle bracket 42 includes a guide block 421, a mounting base plate 422, a column 423, and an air nozzle fixing rod 424. The guide block 421 is disposed on the housing 10 and has a guide groove 425. The mounting base plate 422 is detachably disposed on the housing 10 and partially embedded in the guide groove 425. The column 423 is disposed on the mounting base plate 422 and its extending direction is perpendicular to the extending direction of the guide groove 425. The air nozzle fixing rod 424 is sleeved on the column 423.

[0045] The guide groove 425 is located in the area between the guide block 421 and the housing 10. That is, part of the guide block 421 can be located above the guide groove 425, so that when the mounting base plate 422 is partially embedded in the guide groove 425, it can press down on the mounting base plate 422, thus playing a certain role in auxiliary fixation. The mounting base plate 422 can be detachably connected to the housing 10. When it is necessary to adjust the position of the air nozzle 41, the mounting base plate 422 can be removed and then moved along the guide groove 425.

[0046] The nozzle fixing rod 424 includes a mounting hole 426 and an adjustable gap 427 connecting the mounting hole 426. The mounting hole 426 of the nozzle fixing rod 424 is fitted onto the column 423 and has a clearance fit with the column 423. The adjustable gap 427 divides the nozzle fixing rod 424 into two parts, and the nozzle fixing rod 424 is provided with a fifth locking screw 428 passing through the two parts. By tightening the fifth locking screw 428, the adjustable gap 427 can be reduced, thereby reducing the size of the mounting hole 426 in the direction perpendicular to the adjustable gap 427, so that the nozzle fixing rod 424 can clamp the column 423 and be fixed to the column 423. When the fifth locking screw 428 is loosened, the adjustable gap 427 can be increased, so that the mounting hole 426 and the column 423 return to a clearance fit, thereby allowing the height of the nozzle fixing rod 424 to be adjusted. Of course, after the height of the air nozzle fixing rod 424 is adjusted, the fifth locking screw 428 can be tightened again to fix the air nozzle fixing rod 424.

[0047] See Figure 1 , Figure 2 and Figure 11 The animal restraint device 20 is movably mounted on the housing 10, and its direction of movement is perpendicular to the distance between the animal restraint device 20 and the imaging device 50. For example, the animal restraint device 20 can be movably mounted on the housing 10 using a slider-rail or screw-nut structure. In this application, the slider is a dovetail slider, and the rail is a dovetail groove. In this case, the relative position of the animal restraint device 20 with the air nozzle 41 and the water nozzle 300 can also be appropriately adjusted by moving the animal restraint device 20.

[0048] See Figure 11 The animal restraint device 20 includes an animal compartment 21 and a clamp 22 disposed in the animal compartment 21. The animal compartment 21 is used to contain the animal and restrict the animal's range of motion. The clamp 22 is used to restrict the animal's head movement. The clamp 22 includes a crossbar 221 and a locking assembly 222 for locking the crossbar 221 to the animal compartment 21.

[0049] The animal compartment 21 has a connecting plate 211 with an adjustment hole 212, which is elongated. The purpose of this design is to allow the gripper 22 to be fixed in different positions in the animal compartment 21 according to the size of the mouse.

[0050] The locking assembly 222 includes a sixth locking screw 223 and a locking latch 224. The sixth locking screw 223 is threadedly connected to the animal compartment 21. The locking latch 224 includes an upper latch 225 and a lower latch 226 spaced apart. The upper latch 225 and the lower latch 226 have aligned through holes 227. At least one of the through holes 227 in the upper latch 225 and the lower latch 226 is a threaded hole for threaded connection with the sixth locking screw 223. The spacing between the upper latch 225 and the lower latch 226 is equal to the thickness of the connecting plate 211, and when the connecting plate 211 is located between the upper latch 225 and the lower latch 226, the through holes 227 of the upper latch 225, the through holes 227 of the lower latch 226, and the adjusting hole 212 are aligned.

[0051] The assembly method of this structure is as follows: First, the locking buckle 224 is snapped onto the connecting plate 211, so that the through holes 227 and adjustment holes 212 of the upper plate 225 and lower plate 226 of the locking buckle 224 are aligned. Then, the sixth locking screw 223 is passed through the through hole 227 of the upper plate 225, the adjustment hole 212 and the through hole 227 of the lower plate 226 in sequence. When the sixth locking screw 223 is not fully tightened, it can move in the adjustment hole 212. When it moves to the appropriate position, the sixth locking screw 223 is tightened, and then the fixation is completed.

[0052] The air nozzle 41 is located above the water nozzle 300, and the imaging device 50 is located below the water nozzle 300. This structure is well-suited to the physiological structure of mice. The positions of the animal restraint device 20, air supply device 40, water supply device 30, and imaging device 50 can all be adjusted, thereby adjusting the relative positions between each device to suit the needs of mice of different sizes. Simultaneously, the position of the water nozzle 41 in the left-right direction is automatically adjusted by the drive motor 312 to reduce stimulation to the mice and facilitate the experiment.

[0053] See Figure 12 The enclosure 10 includes a main body 11 and a door 12 hinged to the main body 11. An animal restraint device 20, an air supply device 40, a water supply device 30, and an imaging device 50 are disposed in the main body 11. The main body 11 includes an outer enclosure 13, a sound-absorbing layer 14, and an inner enclosure 15. The outer enclosure 13 is made of metal. The inner enclosure 15 is made of plastic. The sound-absorbing layer 14 includes sound-absorbing foam to reduce the impact of external noise on the mice. The inner enclosure 15 has multiple through holes to allow sound generated inside the enclosure to be absorbed by the sound-absorbing layer 14.

[0054] Finally, it should be noted that the above embodiments are mainly used to illustrate the technical solutions of this application and should not be construed as limiting this application. The foregoing embodiments exemplarily provide a detailed and specific description of this application. Those skilled in the art can modify the technical solutions described in the foregoing embodiments or replace some or all of the technical features therein. However, these modifications or substitutions do not make the corresponding technical solutions and the technical solutions of this application constitute different inventions, and therefore should all be covered within the scope of the claims and specification of this application. In particular, in the absence of structural conflicts or binding obstacles, the various technical features mentioned in the embodiments can be combined in any way, and the technical solutions formed by these combinations should not be considered to have departed from the scope of the technical solutions of this application.

Claims

1. An olfactometer behavior chamber, characterized by, The box and the animal fixing device, the air supply device, the water supply device and the imaging device arranged on the box, the moving area of the head of the animal fixed on the animal fixing device is the experimental area, the air nozzle of the air supply device and the water nozzle of the water supply device are arranged towards the experimental area, and the imaging device is used for collecting images of the experimental area. The water supply device comprises a water nozzle and an adjusting base, the adjusting base comprises a first guide, a mounting piece and a driving motor, the first guide is arranged on the box, the mounting piece is slidably arranged on the first guide, and the driving motor is arranged on the box and used for driving the mounting piece to move along the first guide, and the water nozzle is arranged on the mounting piece.

2. The olfactory experiment behavior box according to claim 1, wherein The mounting piece comprises a sliding block, a second guide and a mounting block assembly, the sliding block is slidably arranged on the first guide, the second guide is fixedly arranged on the sliding block and extends perpendicularly to the first guide, and the mounting block assembly is slidably arranged on the second guide. The mounting block assembly comprises a first screw hole and a first locking screw, the first locking screw is arranged in the first screw hole and limits the relative movement between the mounting block assembly and the second guide when abutting against the second guide.

3. The olfactory experiment behavior box according to claim 2, wherein The second guide is provided with a first groove, the first groove extends parallel to the extension direction of the second guide, the projection of the first screw hole falls into the first groove, and the first locking screw is configured to limit the relative movement between the mounting block assembly and the second guide when abutting against the first groove.

4. The olfactory experiment behavior box according to claim 2, wherein The mounting block assembly comprises a first mounting block, a second mounting block and a second locking screw, the first mounting block is arranged on the second guide and is provided with a second groove, the second groove, the second guide and the first guide extend perpendicularly two by two, the second mounting block is arranged in the second groove and is provided with a first long slot, the length direction of the first long slot is parallel to the extension direction of the second groove, and the second locking screw is arranged in the first long slot and is threadedly connected with the first mounting block, so that the second mounting block can be fixed to the first mounting block.

5. The olfactory experiment behavior box according to claim 1, wherein ​ The imaging device comprises a camera support, a camera assembly, and a third locking screw, the camera support comprises an arc-shaped hole configured to be arranged around a preset axis, the preset axis is perpendicular to the interval direction between the animal fixing device and the imaging device, the third locking screw is arranged in the arc-shaped hole and is threadedly connected with the camera assembly, and is configured to fix the camera assembly and the camera support in a locked state and to rotate the camera assembly relative to the camera support around the preset axis in an unlocked state. And / or, The imaging device comprises a camera support, a camera assembly, and a fourth locking screw, the camera support has two second long holes, the length direction of the second long holes is parallel to the interval direction between the animal fixing device and the imaging device, and the fourth locking screw is arranged in the second long holes and is threadedly connected with the box.

6. The olfactory experimental behavior box according to claim 1, wherein The air supply device comprises an air nozzle and an air nozzle support, the air nozzle support comprises a guide block, a mounting bottom plate, a stand, and an air nozzle fixing rod, the guide block is arranged on the box and has a guide groove, the mounting bottom plate is detachably arranged on the box and is partially embedded in the guide groove, the stand is arranged on the mounting bottom plate and its extension direction is perpendicular to the extension direction of the guide groove, and the air nozzle fixing rod is sleeved on the stand.

7. The olfactory experimental behavior box according to claim 1, wherein The animal fixing device is movably arranged on the box and its moving direction is perpendicular to the interval direction between the animal fixing device and the imaging device.

8. The olfactory experimental behavior box according to claim 1, wherein The animal fixing device comprises an animal cabin and a clamping device arranged on the animal cabin, and the clamping device comprises a horizontal rod and a locking assembly for locking the horizontal rod in the animal cabin.

9. The olfactory experimental behavior box according to claim 8, wherein The animal cabin has a connecting plate, the connecting plate is provided with an adjusting hole in the form of a long slot; The locking assembly comprises a sixth locking screw and a locking buckle, the locking buckle comprises a top clamping plate and a bottom clamping plate arranged at intervals, the top clamping plate and the bottom clamping plate are provided with through holes arranged in alignment, at least one of the through holes in the top clamping plate and the bottom clamping plate is a threaded hole for threadedly connecting with the sixth locking screw, The interval distance between the top clamping plate and the bottom clamping plate is equal to the thickness of the connecting plate, when the connecting plate is located between the top clamping plate and the bottom clamping plate, the through hole of the top clamping plate, the through hole of the bottom clamping plate, and the adjusting hole are aligned.

10. The olfactory experimental behavior box according to claim 1, wherein The air nozzle is located above the water nozzle, and the imaging device is located below the water nozzle, And / or, The box comprises a main body part and a door hinged to the main body part, the animal fixing device, the air supply device, the water supply device and the imaging device are arranged in the main body part, the main body part comprises an outer box body, a sound absorbing layer and an inner box body; the inner box body is provided with a plurality of through holes, so that the sound generated inside the box can be absorbed by the sound absorbing layer.

Images