Counting plate
By setting an overflow chamber in the counting chamber that is connected to the counting chamber, the problem of insufficient sample liquid caused by air bubbles in the counting chamber is solved, thus achieving stability of sample liquid volume and accuracy of detection results.
Patent Information
- Application Number
- CN202423228575.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-26
- Publication Date
- 2025-12-30
- Estimated Expiration
- 2034-12-26
AI Technical Summary
Existing cell counting chambers are prone to air bubbles in the counting chamber, resulting in insufficient sample volume and affecting counting accuracy.
Design a counting chamber that connects the counting chamber to the overflow chamber, allowing the sample liquid to exceed the required volume of the counting chamber. The overflow chamber receives the excess liquid, avoiding the influence of air bubbles and ensuring the stability of the sample liquid volume.
The overflow chamber design ensures that the sample solution in the counting chamber is completely filled, reducing air bubbles and improving the accuracy of the test results.
Smart Images
Figure CN223738043U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of testing equipment technology, and in particular to a counting plate. Background Technology
[0002] In biological research and testing, it is sometimes necessary to count the number of cells per unit volume, which is usually achieved using a cell counting chamber. Existing cell counting chambers consist of a housing and a substrate disposed within a recessed space in the housing. A gap exists between the bottom surface of the recessed space and the substrate; this gap space is the counting chamber of the cell counting chamber. A liquid sample is introduced into this counting chamber, and the cells are counted using a microscope, image acquisition device, or similar means.
[0003] However, due to the small volume of the counting chamber, air bubbles may appear in the counting chamber when the sample solution is injected, due to the different physicochemical properties of the sample solution and the uncontrollability of the injection process. This may result in the counting chamber not being completely filled with sample solution, and the actual volume of the sample solution being less than the rated volume of the counting chamber, which will have an adverse effect on the accuracy of the counting. Utility Model Content
[0004] To solve the above-mentioned technical problems, the purpose of this utility model is to provide a counting plate. The counting plate provided in this application has a counting chamber connected to an overflow chamber to receive excess sample liquid. The amount of sample liquid injected can be more than the volume required by the counting chamber, avoiding the influence of factors such as air bubbles, ensuring the stability of the sample liquid volume in the counting chamber, and improving the accuracy of the detection results.
[0005] The technical solution provided by this utility model is as follows:
[0006] A counting board includes a housing and a substrate.
[0007] The box body is recessed downward to form a first plane, and a sidewall is formed on the outer periphery of the first plane;
[0008] The substrate covers the first plane, and the gap between the substrate and the first plane forms a counting cell cavity;
[0009] The counting chamber is connected to the injection port;
[0010] The counting chamber is also connected to the overflow chamber.
[0011] Preferably, an injection port and an overflow chamber are arranged along the first direction, and the sample liquid enters through the injection port, passes through the counting chamber, and enters the overflow chamber.
[0012] Preferably, the height of the bottom surface of the overflow cavity is lower than the height of the first plane.
[0013] Preferably, the substrate extends above the overflow cavity.
[0014] Preferably, the first plane is recessed downwards to form an injection cavity;
[0015] The substrate covers the liquid injection cavity.
[0016] Preferably, the housing is provided with a first limiting member, which is used to limit the position of the substrate, and the first limiting member at least partially extends into the overflow cavity; or,
[0017] The box body is provided with a first limiting member and a second limiting member. The first limiting member is used to limit the position of the substrate and extends at least partially into the overflow cavity. The distance between the second limiting member and the first limiting member is greater than or equal to the size of the substrate placed therebetween.
[0018] Preferably, protrusions are provided on both sides of the first plane, the protrusions are located on the inner side of the side wall, and the substrate is supported by the top surface of the protrusions to form a gap between the substrate and the first plane.
[0019] Preferably, an injection groove is formed between the boss and the sidewall.
[0020] Preferably, the height of the sidewall is higher than the height of the boss.
[0021] Preferably, the substrate is a transparent sheet; and / or,
[0022] The bottom surface of the box is provided with a support protrusion.
[0023] To address the problems existing in the prior art, this application provides a counting plate, including a housing and a substrate. The housing is recessed downwards to form a first plane, and a sidewall is formed on the outer periphery of the first plane. The substrate covers the first plane, and the gap between the substrate and the first plane forms a counting chamber. The counting chamber is connected to an injection port and also to an overflow chamber. The counting plate provided in this application has a counting chamber connected to an overflow chamber to receive excess sample liquid. The amount of sample liquid injected can exceed the volume required by the counting chamber, avoiding the influence of factors such as air bubbles, ensuring the stability of the sample liquid volume within the counting chamber, and improving the accuracy of the detection results. Attached Figure Description
[0024] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0025] Figure 1 This is a schematic diagram of the counting plate (combination of substrate and box) in an embodiment of the present invention.
[0026] Figure 2 This is a schematic diagram of the counting plate in an embodiment of the present invention (the substrate and the box are separate).
[0027] Figure 3 This is a top view of the counting plate (combination of substrate and box) in an embodiment of the present invention.
[0028] Figure 4 for Figure 3 Sectional view along axis AA;
[0029] Figure 5 for Figure 3 BB-direction sectional view;
[0030] Figure 6 for Figure 5 A magnified view of the area within the middle circle;
[0031] Figure 7 This is a schematic diagram of the box body in an embodiment of the present utility model;
[0032] Figure 8 for Figure 7 A magnified view of the area within the middle circle;
[0033] Figure 9 This is a schematic diagram of the bottom structure of the box in an embodiment of the present utility model;
[0034] Reference numerals: 1-box body; 11-first plane; 12-side wall; 13-support protrusion; 2-substrate; 3-counting cell cavity; 4-overflow cavity; 5-liquid injection cavity; 6-first limiting member; 7-second limiting member; 8-bore; 9-glue injection groove. Detailed Implementation
[0035] To enable those skilled in the art to better understand the technical solutions in this application, the technical solutions in the embodiments of this application will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.
[0036] It should be noted that when a component is referred to as being "fixed to" or "set on" another component, it can be directly on or indirectly set on the other component; when a component is referred to as being "connected to" another component, it can be directly connected to or indirectly connected to the other component.
[0037] It should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.
[0038] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, "a plurality of" or "several" means two or more, unless otherwise explicitly specified.
[0039] It should be noted that the structures, proportions, sizes, etc., shown in the accompanying drawings of this specification are only for the purpose of assisting those skilled in the art in understanding and reading the content disclosed in the specification, and are not intended to limit the conditions under which this application can be implemented. Therefore, they have no substantial technical significance. Any modifications to the structure, changes in the proportions, or adjustments to the size should still fall within the scope of the technical content disclosed in this application, provided that they do not affect the effects and purposes that this application can produce.
[0040] As shown in the figure, this embodiment of the utility model provides a counting board, including a box body 1 and a substrate 2.
[0041] The box body 1 is recessed downward to form a first plane 11, and a side wall 12 is formed on the outer periphery of the first plane 11;
[0042] The substrate 2 covers the first plane 11, and the gap between the substrate 2 and the first plane 11 forms the counting cell cavity 3;
[0043] The counting chamber 3 is connected to the injection port;
[0044] The counting chamber 3 is also connected to an overflow chamber 4.
[0045] To address the problems existing in the prior art, this application provides a counting plate, including a housing 1 and a substrate 2. The housing 1 is recessed to form a first plane 11, and a sidewall 12 is formed on the outer periphery of the first plane 11. The substrate 2 covers the first plane 11, and the gap between the substrate 2 and the first plane 11 forms a counting chamber 3. The counting chamber 3 is connected to the injection port and also to the overflow chamber 4. The counting plate provided in this application has the counting chamber 3 connected to the overflow chamber 4 to receive excess sample liquid. The amount of sample liquid injected can be greater than the required volume of the counting chamber 3, avoiding the influence of factors such as air bubbles, ensuring the stability of the sample liquid volume in the counting chamber 3, and improving the accuracy of the detection results.
[0046] Specifically, when using the counting chamber provided in this application, sample solution is injected into the counting chamber 3 through the injection port. The injection port and overflow chamber 4 are both connected to the outside atmosphere. The air originally inside the counting chamber 3 is discharged into the outside atmosphere through the connected overflow chamber 4. This process reduces air bubbles in the counting chamber 3, thus ensuring the entire counting chamber 3 is filled with sample solution. Furthermore, due to the overflow chamber 4, the volume of injected sample solution can be greater than the preset capacity of the counting chamber 3. As sample solution is continuously injected, excess sample solution flows into the overflow chamber 4 for storage (for example, if the preset volume of the counting chamber 3 is 2 ml, the injected sample solution can be 2.2 ml or 2.5 ml, with an excess of 0.2 or 0.5 ml flowing into the overflow chamber 4). In this case, the accuracy requirement for injection volume can be reduced.
[0047] Preferably, the injection port and the overflow chamber 4 are arranged along the first direction. The sample liquid enters through the injection port, passes through the counting chamber 3, and enters the overflow chamber 4.
[0048] There is a gap between the substrate 2 and the first plane 11. The naturally formed opening can serve as a liquid injection port, or a through hole can be formed on the substrate 2 as a liquid injection port. The liquid injection port can be located at any position above or to the side of the first plane 11, as long as it is connected to the counting cell cavity 3 for sample liquid injection.
[0049] Preferably, an injection port and an overflow chamber 4 are arranged along the first direction. The sample liquid enters through the injection port, passes through the counting chamber 3, and enters the overflow chamber 4, ensuring that the counting chamber 3 is filled first and then enters the overflow chamber 4 when the sample liquid is injected.
[0050] More preferably, the first plane 11 is rectangular, and the injection port and the overflow cavity 4 are located at the two ends of the first plane 11 respectively. When the sample liquid is injected, the sample liquid flows along the first direction to naturally fill the counting cell cavity 3 and then flows into the overflow cavity 4.
[0051] Preferably, the height of the bottom surface of the overflow cavity 4 is lower than the height of the first plane 11.
[0052] Depending on the method of sample injection, the height of the bottom surface of the overflow cavity 4 can have different relationships with the height of the first plane 11.
[0053] When using the injection method, the sample liquid has the power to flow due to the pressure applied during injection. The height of the bottom surface of the overflow cavity 4 can be flush with, lower than or higher than the first plane 11, and excess sample liquid can flow into the overflow cavity 4. However, setting the height of the bottom surface of the overflow cavity 4 to be lower than the first plane 11 is also more conducive to the flow of sample liquid.
[0054] When a flow-guided flow method is used, the flow of the sample liquid relies not only on the driving force provided by the continuous injection of liquid occupying the space, but also on the surface tension between the liquid and the substrate 2 or the housing 1. In this case, it is preferable to set the height of the bottom surface of the overflow cavity 4 to be lower than the height of the first plane 11, so that excess sample liquid can flow into the overflow cavity 4.
[0055] Preferably, the substrate 2 extends above the overflow cavity 4.
[0056] If the substrate 2 extends above the overflow cavity 4, the surface tension between the substrate 2 and the sample liquid can cause the sample liquid to flow along the substrate 2 and fall into the overflow cavity 4.
[0057] Preferably, the first plane 11 is recessed downward to form an injection cavity 5;
[0058] The substrate 2 covers part of the liquid injection cavity 5.
[0059] Preferably, the first plane 11 is recessed downwards to form the injection cavity 5. The substrate 2 covers a portion of the injection cavity 5, and the sample liquid is injected through this cavity. The sample liquid naturally flows into the portion of the injection cavity 5 covered by the substrate 2, completing the injection process. Because the injection cavity 5 is recessed downwards, the sample liquid is buffered by the injection cavity 5 during injection, which can avoid problems such as droplet splashing that could affect the instrument's lifespan.
[0060] The injection chamber 5 preferably has an arc-shaped bottom to further reduce the probability of splashing.
[0061] Preferably, the housing 1 is provided with a first limiting member 6, which is used to limit the position of the substrate 2, and the first limiting member 6 extends at least partially into the overflow cavity 4; or,
[0062] The box body 1 is provided with a first limiting member 6 and a second limiting member 7. The first limiting member 6 is used to limit the position of the substrate 2, and the first limiting member 6 extends at least partially into the overflow cavity 4. The distance between the second limiting member 7 and the first limiting member 6 is greater than or equal to the size of the substrate 2 placed therebetween.
[0063] The preferred box body 1 is equipped with a limiting component. The limiting component can be a single first limiting component 6, or a first limiting component 6 and a second limiting component 7 can be provided simultaneously.
[0064] Specifically, when the first limiting member 6 is set separately, since the first limiting member 6 extends at least partially into the overflow cavity 4, the substrate 2 naturally extends into the overflow cavity 4 when it abuts against the first limiting member 6.
[0065] When the first limiting member 6 and the second limiting member 7 are set at the same time, the distance between the first limiting member 6 and the second limiting member 7 is greater than or equal to the size of the substrate 2 placed between them. When it is equal to the size, the substrate 2 is just embedded. When it is greater than the size, the substrate 2 is easier to put in and install. After it is put in, the position of the substrate 2 can be adjusted, or the distance between the two limiting members 6 can be used to fix the substrate 2 between them to meet the usage requirements.
[0066] More preferably, the first limiting member 6 and the second limiting member 7 are located at opposite ends of the substrate 2 along its length, such that the distance between the first limiting member 6 and the second limiting member 7 is greater than or equal to the length of the substrate 2. The number of the first limiting member 6 and the second limiting member 7 can be arbitrarily set to one, two, or more as needed.
[0067] Preferably, protrusions 8 are provided on both sides of the first plane 11. The protrusions 8 are located inside the side wall 12, and the substrate 2 is supported by the top surface of the protrusions 8 to form a gap between the substrate 2 and the first plane 11.
[0068] The gap between the first plane 11 and the substrate 2 can be fixed by the substrate 2 being held in place by the side wall of the housing 1. More preferably, protrusions 8 are provided on both sides of the first plane 11. The protrusions 8 are located inside the side wall 12 of the housing 1, and the substrate 2 is supported by the top surface of the protrusions 8 to form a gap between the substrate 2 and the first plane 11. The height of this gap is the height of the protrusions 8 relative to the first plane 11, which is also the height of the liquid injection chamber 5. By controlling the height of the protrusions 8 during processing, the height of the counting chamber 3 can be easily determined.
[0069] The extension direction of the boss 8 can be parallel to the first direction or at an angle of less than 90 degrees to the first direction, both of which can form a counting cell cavity 3 between the two bosses 8. Considering the ease of manufacturing, the ease of operation of the counting plate, and the angle of avoiding the bosses 8 from blocking the best observation field of view in the middle of the counting cell cavity 4, it is preferable that the extension direction of the boss 8 is parallel to the first direction.
[0070] Preferably, an injection groove 9 is formed between the boss 8 and the side wall 12.
[0071] More preferably, a glue injection groove 9 is formed between the boss 8 and the side wall 12; then, during the manufacturing process of the counting plate, adhesive is injected into the glue injection groove 9, and then the substrate 2 is placed on the boss 8. The substrate 2 contacts the adhesive and is then firmly fixed as the adhesive cures, thereby achieving the connection between the substrate 2 and the box 1.
[0072] Preferably, the height of the sidewall 12 is higher than the height of the boss 8.
[0073] Preferably, the height of the side wall 12 of the housing 1 is higher than the height of the boss 8. The side wall 12 surrounds the first plane 11, acting as the last line of defense to prevent the sample liquid from overflowing the counting plate. Even if the liquid injection speed is fast, the liquid that accumulates instantly during injection will be blocked by the side wall 12 and will not overflow.
[0074] Preferably, the substrate 2 is a transparent sheet; and / or,
[0075] The bottom surface of the box 1 is provided with a support protrusion 15.
[0076] Preferably, the substrate 2 is a transparent sheet, which will not have an adverse effect on the observation of target objects such as cells through the substrate 2.
[0077] More preferably, the substrate 2 is made of plastic or glass, which has a strong surface tension with the sample liquid, thus facilitating the diffusion of the sample liquid. Whether the sample liquid is injected by pressure injection or by flow-guided injection, surface tension helps the sample liquid diffuse. However, compared to pressure injection, surface tension has a greater effect on flow-guided injection.
[0078] Specifically, due to differences in the physicochemical properties of the sample solutions, their surface tensions may also differ. When the surface tension is weak, the amount of sample solution injected can be adjusted to ensure that the counting chamber 3 is filled. Specifically, the total amount of sample solution injected ensures that the liquid level in the injection chamber 5 (when set), the counting chamber 3, and the overflow chamber 4 is equal to the bottom height of the substrate 2, thus ensuring that the counting chamber 3 is filled, regardless of whether the surface tension of the sample solution is good or bad.
[0079] When the surface tension of the sample liquid is good, using a plastic or glass substrate 2 can utilize the good surface tension and flow guiding effect of the substrate 2 to allow the sample liquid to diffuse smoothly and fill the counting chamber 3. At this time, the overflow chamber 4 and the liquid injection chamber 5 are located outside the substrate 2, and their liquid level can be lower than the bottom surface of the substrate 2. At this time, the liquid in the counting chamber 3 will diffuse between the substrate 2 and the first plane, thus ensuring that the sample liquid filling amount in the counting chamber 3 meets the detection requirements.
[0080] The bottom surface of the preferred box 1 is provided with a support protrusion 15 to support the entire counting plate and keep it horizontal, so as to facilitate counting and reduce the impact of unevenness of the surface on which it is placed (such as the surface of a conveyor platform or conveyor belt).
[0081] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. A counting plate, characterized in that, The box body (1) is downwardly recessed to form a first plane (11), and a side wall (12) is formed around the first plane (11). The substrate (2) covers the first plane (11), and a gap between the substrate (2) and the first plane (11) forms a counting pool cavity (3). The counting pool cavity (3) is in communication with the liquid injection port. The counting pool cavity (3) is also in communication with an overflow cavity (4). The liquid injection port and the overflow cavity (4) are arranged in a first direction, and sample liquid enters the overflow cavity (4) through the counting pool cavity (3) from the liquid injection port.
2. The counting plate of claim 1, wherein The height of the bottom surface of the overflow cavity (4) is lower than the height of the first plane (11).
3. The counting plate of claim 1, wherein, The substrate (2) extends above the overflow cavity (4).
4. The counting plate of claim 1, wherein, The first plane (11) is downwardly recessed to form a liquid injection cavity (5).
5. The counting plate of claim 1, wherein, The substrate (2) covers part of the liquid injection cavity (5). The box body (1) is provided with a first limiting member (6) for limiting the position of the substrate (2), and the first limiting member (6) at least partially extends into the overflow cavity (4); or, 6. The counting plate according to any one of claims 1-5, wherein, The box body (1) is provided with a first limiting member (6) and a second limiting member (7), the first limiting member (6) is used for limiting the position of the substrate (2), and the first limiting member (6) at least partially extends into the overflow cavity (4); the distance between the second limiting member (7) and the first limiting member (6) is greater than or equal to the size of the substrate (2) placed therebetween. The first plane (11) is provided with a boss (8) on both sides, the boss (8) is located inside the side wall (12), and the substrate (2) is supported by the top surface of the boss (8) to form a gap between the substrate (2) and the first plane (11).
7. The counting plate of claim 1, wherein, The boss (8) and the side wall (12) form a glue injection groove (9).
8. The counting plate of claim 7, wherein, The height of the side wall (12) is higher than the height of the boss (8).
9. The counting plate of claim 7, wherein, The substrate (2) is a transparent sheet; and / or, 10. The counting plate according to any one of claims 1-5, 7-9, wherein, The bottom surface of the box body (1) is provided with a supporting protrusion (13).