Culture dish convenient for taking and placing cell climbing slide
By introducing structures such as U-shaped plates and arc plates into the culture dish, the problems of difficulty in removing cell slides and leakage of culture medium were solved, achieving convenient handling and leakage prevention.
Patent Information
- Application Number
- CN202422732995.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-11
- Publication Date
- 2025-12-30
- Estimated Expiration
- 2034-11-11
AI Technical Summary
The existing culture dishes have small gaps between the cell slides and the dish walls, making them difficult to remove and easy to damage. Furthermore, the culture medium is prone to leakage, which can contaminate the lab workbench.
A culture dish with structures including a U-shaped plate, an arc plate, a pressing plate, and a reset spring was designed. Through the cooperation of these structures, the dish wall and the bottom can be separated to expose the cell slabs. The culture medium is automatically collected through a trapezoidal sealing block and an inlet hole to prevent leakage.
It enables convenient removal of cell slides, avoids breakage, and prevents culture medium leakage from contaminating the experimental table, thus improving the convenience of operation and cleaning efficiency.
Smart Images
Figure CN223738049U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of culture dish technology, specifically to a culture dish that facilitates the placement and removal of cell slides. Background Technology
[0002] A culture dish is a commonly used laboratory instrument used in cell culture and related research. During experiments, culture dishes are generally used in conjunction with cell slides. The cell slides are placed in the culture compartment of the culture dish, immersing them in cell culture medium. Cells grow on the cell slides, facilitating histological, immunohistochemical, frozen section, cell smear, and in situ hybridization studies.
[0003] However, due to the small gap between the cell slide and the culture dish wall, it is quite troublesome for researchers to remove the cell slide from the culture dish. During the process of picking it up, the slide is easy to fall back into the dish repeatedly, and excessive force when picking it up can easily cause the slide to break. Utility Model Content
[0004] The main objective of this invention is to provide a culture dish that facilitates the handling and placement of cell slides, thereby overcoming or improving at least one technical problem of the prior art, or providing a useful alternative.
[0005] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:
[0006] A culture dish for easy handling of cell slides includes a dish bottom, a dish wall attached to the surface of the dish bottom, U-shaped plates fixedly connected to both sides of the dish wall, and arc-shaped plates attached to both the inner wall of the U-shaped plates and the surface of the dish wall. A pressing plate is fixedly connected to the front of the arc-shaped plates, a first hollow block is fixedly connected to the back of the arc-shaped plates, a return spring is fixedly connected to the inner wall of the first hollow block, a second hollow block is fixedly connected to the side of the arc-shaped plates, and an L-shaped column is fixedly connected to the inner wall of the second hollow block. Arc-shaped columns are fixedly connected to both sides of the dish bottom, with the upper and lower sides of the arc-shaped columns contacting the bottom of the arc-shaped plates and the upper surface of the L-shaped columns, respectively. A strip-shaped hole is formed inside the U-shaped plates, and a limiting block is fixedly connected to the side of the arc-shaped plates, with the surface of the limiting block contacting the inner wall of the strip-shaped hole.
[0007] Furthermore, the bottom of the dish includes a base plate, and a circular box is fixedly connected to the upper surface of the base plate. The surface of the circular box is in contact with the inner wall of the dish wall. The left and right sides of the circular box are fixedly connected to the surface of the arc-shaped column. A liquid inlet hole is opened on the surface of the circular box. The inner wall of the liquid inlet hole is in contact with the surface of the dish wall. A protrusion is fixedly connected to the upper surface of the circular box.
[0008] Furthermore, the vessel wall includes an inner cylinder, a trapezoidal sealing block is fixedly connected to the surface of the inner cylinder, an outer cylinder is fixedly connected to the side of the trapezoidal sealing block away from the inner cylinder, the inner wall of the outer cylinder is in contact with the surface of the circular box, the surface of the trapezoidal sealing block is in contact with the inner wall of the liquid inlet, the left and right sides of the outer cylinder are fixedly connected to the side of the U-shaped plate, and the left and right sides of the outer cylinder are in contact with the surface of the arc-shaped plate.
[0009] Furthermore, a rubber ring is adhered to the bottom of the inner cylinder, and the bottom of the rubber ring is in contact with the upper surface of the circular box.
[0010] Furthermore, a first circular hole is provided inside the base plate, and a second circular hole is provided on the upper surface of the circular box. A sealing plug is fitted to the inner wall of the second circular hole and the upper surface of the base plate. A threaded ring is threaded to the surface of the sealing plug, and a column is fixedly connected to the bottom of the threaded ring and the upper surface of the base plate.
[0011] Furthermore, the centers of the first circular hole, the second circular hole, the base plate, and the circular box are all on the same vertical line, and the diameter of the first circular hole is smaller than the diameter of the second circular hole.
[0012] Compared with the prior art, the present invention has the following beneficial effects:
[0013] The combination of a U-shaped plate, an arc-shaped plate, a pressing plate, a first hollow block, a reset spring, a second hollow block, an L-shaped column, an arc-shaped column, a strip-shaped hole, and a limiting block allows the bottom of the dish and the dish wall to be assembled together or separated. At the same time, when the dish wall and the dish bottom are separated, the cell spreaders on the dish bottom can be directly exposed. The protrusions on the dish bottom create a certain distance between the circular box and the cell spreaders. At this time, the experimenter can easily use tweezers to grasp the cell spreaders, making it easier to remove the cell spreaders from the culture dish and less likely to damage them.
[0014] The trapezoidal sealing block on the dish wall can seal the liquid inlet hole on the bottom of the dish. At the same time, when the L-shaped column separates from the arc-shaped column and the dish wall moves upward, the liquid inlet hole can automatically open, allowing the culture medium inside the dish wall to automatically flow into the circular box. Then, when the dish wall is separated from the bottom, the culture medium can be automatically collected, so that the culture medium inside the dish will not flow onto the experimental table and cause contamination of the experimental table. Attached Figure Description
[0015] Figure 1 This is a front view of the structure of this utility model.
[0016] Figure 2 This is a right view of the structure of this utility model.
[0017] Figure 3 for Figure 2Sectional view at point AA.
[0018] Figure 4 This is a top view of the structure of this utility model.
[0019] Figure 5 This is a bottom view of the structure of this utility model.
[0020] Figure 6 This is a top view of the bottom of the dish of this utility model.
[0021] Figure 7 for Figure 6 Sectional view at point BB.
[0022] Figure 8 This is a top view of the dish wall in this utility model.
[0023] Figure 9 This is a bottom view of the threaded ring in this utility model.
[0024] Among them, 1. Dish bottom; 101. Base plate; 102. Circular box body; 103. Liquid inlet hole; 104. Protrusion; 2. Dish wall; 201. Inner cylinder; 202. Trapezoidal sealing block; 203. Outer cylinder; 3. U-shaped plate; 4. Arc-shaped plate; 5. Pressing plate; 6. First hollow block; 7. Return spring; 8. Second hollow block; 9. L-shaped column; 10. Arc-shaped column; 11. Strip hole; 12. Limiting block; 13. Rubber ring; 14. First round hole; 15. Second round hole; 16. Sealing plug; 17. Threaded ring; 18. Column. Detailed Implementation
[0025] The technical solution of this utility model will be further described below with reference to the accompanying drawings and embodiments.
[0026] Combination Figures 1 to 9 This embodiment provides a culture dish for easy handling of cell slides, including a dish bottom 1, a dish wall 2 attached to the surface of the dish bottom 1, U-shaped plates 3 fixedly connected to both sides of the dish wall 2, and arc-shaped plates 4 attached to both the inner wall of the U-shaped plates 3 and the surface of the dish wall 2. The U-shaped plates 3 guide the movement trajectory of the arc-shaped plates 4. A pressing plate 5 is fixedly connected to the front of the arc-shaped plates 4, a first hollow block 6 is fixedly connected to the back of the arc-shaped plates 4, a return spring 7 is fixedly connected to the inner wall of the first hollow block 6, a second hollow block 8 is fixedly connected to the side of the arc-shaped plates 4, an L-shaped column 9 is fixedly connected to the inner wall of the second hollow block 8, and arc-shaped columns 10 are fixedly connected to both sides of the dish bottom 1. The upper and lower sides of the arc-shaped columns 10 contact the bottom of the arc-shaped plates 4 and the upper surface of the L-shaped columns 9, respectively.
[0027] When the bottom of the L-shaped column 9 contacts the bottom of the arc-shaped column 10, the dish wall 2 can be fixed to the dish bottom 1 through the cooperation of the arc-shaped column 10 and the L-shaped column 9. When the L-shaped column 9 separates from the arc-shaped column 10, the dish wall 2 can be directly separated from the dish bottom 1. The U-shaped plate 3 has a strip hole 11 inside. The side of the arc-shaped plate 4 is fixedly connected to a limiting block 12. The surface of the limiting block 12 contacts the inner wall of the strip hole 11. Through the cooperation of the strip hole 11 and the limiting block 12, the movement distance of the arc-shaped plate 4 can be limited. At the same time, when the bottom of the L-shaped column 9 contacts the bottom of the arc-shaped column 10, the tension of the first hollow block 6 by the return spring 7 can prevent the L-shaped column 9 and the arc-shaped column 10 from separating automatically.
[0028] The bottom of the petri dish 1 includes a base plate 101. A circular box 102 is fixedly connected to the upper surface of the base plate 101. The surface of the circular box 102 is in contact with the inner wall of the petri dish wall 2. The left and right sides of the circular box 102 are fixedly connected to the surface of the arc-shaped column 10. A liquid inlet hole 103 is opened on the surface of the circular box 102. The inner wall of the liquid inlet hole 103 is in contact with the surface of the petri dish wall 2. When the L-shaped column 9 separates from the arc-shaped column 10 and the petri dish wall 2 moves upward, the liquid inlet hole 103 can automatically open, and the culture medium in the petri dish wall 2 can automatically flow into the circular box 102. Then, when the petri dish wall 2 is separated from the bottom of the petri dish 1, the culture medium can be automatically collected, so that the culture medium in the petri dish will not flow onto the experimental table and cause the experimental table to be contaminated.
[0029] The upper surface of the circular box 102 is fixedly connected with a protrusion 104. When the dish wall 2 is separated from the dish bottom 1, the cell slab on the dish bottom 1 can be directly exposed. Through the protrusion 104 on the dish bottom 1, there is a certain distance between the circular box 102 and the cell slab. At this time, the experimenter can more easily use tweezers to grasp the cell slab, so that the cell slab in the culture dish can be more easily removed.
[0030] The dish wall 2 includes an inner cylinder 201, with a trapezoidal sealing block 202 fixedly connected to the surface of the inner cylinder 201. An outer cylinder 203 is fixedly connected to the side of the trapezoidal sealing block 202 away from the inner cylinder 201. The inner wall of the outer cylinder 203 contacts the surface of the circular box 102, and the surface of the trapezoidal sealing block 202 contacts the inner wall of the liquid inlet 103. When the dish wall 2 is fixed to the dish bottom 1, the trapezoidal sealing block 202 can seal the liquid inlet 103 on the dish bottom 1, and liquid can be introduced through the inlet. The cooperation between the hole 103 and the trapezoidal sealing block 202 prevents the dish wall 2 from rotating on the dish bottom 1. The left and right sides of the outer cylinder 203 are fixedly connected to the sides of the U-shaped plate 3, and the left and right sides of the outer cylinder 203 are in contact with the surface of the arc plate 4. A rubber ring 13 is glued to the bottom of the inner cylinder 201, and the bottom of the rubber ring 13 is in contact with the upper surface of the circular box 102. Through the rubber ring 13, the culture medium in the dish wall 2 will not leak from the connection between the dish wall 2 and the dish bottom 1.
[0031] In another embodiment, a first circular hole 14 is formed inside the base plate 101, and a second circular hole 15 is formed on the upper surface of the circular box 102. The centers of the first circular hole 14, the second circular hole 15, the base plate 101, and the circular box 102 are all on the same vertical line. The diameter of the first circular hole 14 is smaller than the diameter of the second circular hole 15. A sealing plug 16 is fitted to both the inner wall of the second circular hole 15 and the upper surface of the base plate 101. A threaded ring 17 is threaded onto the surface of the sealing plug 16. A column 18 is fixedly connected to the bottom of the threaded ring 17 and the upper surface of the base plate 101. When the sealing plug 16... When the 6 is screwed into the threaded ring 17 and contacts the base plate 101, the sealing plug 16 can seal the first round hole 14 and the second round hole 15. At the same time, when cleaning the inside of the circular box 102, the sealing plug 16 is rotated to separate the sealing plug 16 from the threaded ring 17 and remove the sealing plug 16 from the circular box 102. At this time, the culture medium inside the circular box 102 can be quickly discharged through the first round hole 14. Meanwhile, the cleaning water pipe can be extended into the circular box 102 through the second round hole 15, which facilitates the experimenter to clean the inner wall of the circular box 102.
[0032] In this invention, when using the culture dish, the user first places the cell slide on the protrusion 104, then pinches the two pressing plates 5 on the left and right to minimize the distance between them. Next, the outer cylinder 203 is placed on the circular box 102, and the trapezoidal sealing block 202 is inserted into the liquid inlet 103. After the arc plate 4 contacts the arc column 10, the pressing plates 5 are released, and the L-shaped column 9 is reset by the elastic force of the reset spring 7. At this time, the L-shaped column 9 contacts the bottom surface of the arc column 10, and the dish wall 2 can be fixed on the dish bottom 1 by the cooperation of the arc column 10 and the L-shaped column 9.
[0033] Then, pour the culture medium into the dish wall 2 and culture the cells on the cell slide through the culture medium. After the culture is completed, pinch the two pressing plates 5 on the left and right to separate the L-shaped column 9 from the arc column 10. Then, open the liquid inlet 103 by moving the outer cylinder 203 upward. At this time, the culture medium in the dish wall 2 enters the circular box 102 through the liquid inlet 103.
[0034] Once the outer cylinder 203 is completely separated from the circular box 102, the cell slab on the bottom of the dish 1 can be directly exposed. The protrusion 104 on the bottom of the dish 1 creates a certain distance between the circular box 102 and the cell slab, allowing the experimenter to easily use tweezers to hold the cell slab.
[0035] When cleaning the inner wall of the circular box 102, firstly, by rotating the sealing plug 16, the sealing plug 16 is separated from the threaded ring 17, and the sealing plug 16 is removed from the circular box 102. At this time, the culture medium inside the circular box 102 can be quickly discharged through the first circular hole 14. At the same time, the experimenter inserts the cleaning water pipe into the circular box 102 through the second circular hole 15, and the water flow rinses the inner wall of the circular box 102. After rinsing, the sealing plug 16 is inserted into the second circular hole 15 and screwed into the threaded ring 17. At this time, the sealing plug 16 is fixed in the circular box 102 by the threads, and the first circular hole 14 and the second circular hole 15 are sealed by the sealing plug 16.
[0036] The above description is merely a preferred embodiment of the present utility model and does not constitute any limitation on the technical scope of the present utility model. Therefore, any minor modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present utility model shall still fall within the scope of the technical solution of the present utility model.
Claims
1. A culture dish for easy handling and placement of cell slides, characterized in that, The utility model provides a kind of dish, including dish bottom (1), the surface of dish bottom (1) is provided with dish wall (2) in place, the left and right sides of dish wall (2) are fixedly connected with U-shaped plate (3), the inner wall of U-shaped plate (3) and the surface of dish wall (2) are provided with arc plate (4) in place, the front of arc plate (4) is fixedly connected with pressing plate (5), the back of arc plate (4) is fixedly connected with first hollow block (6), the inner wall of first hollow block (6) is fixedly connected with return spring (7), the side of arc plate (4) is fixedly connected with second hollow block (8), the inner wall of second hollow block (8) is fixedly connected with L-shaped column (9), the left and right sides of dish bottom (1) are fixedly connected with arc column (10), the upper and lower sides of arc column (10) are respectively with the bottom of arc plate (4) and the upper surface of L-shaped column (9) contact, the inside of U-shaped plate (3) is provided with strip hole (11), the side of arc plate (4) is fixedly connected with limit block (12), the surface of limit block (12) and the inner wall of strip hole (11) contact.
2. The cell culture dish of claim 1, wherein, The dish bottom (1) includes a bottom plate (101), the upper surface of the bottom plate (101) is fixedly connected with a circular box body (102), the surface of the circular box body (102) is in contact with the inner wall of the dish wall (2), the left and right sides of the circular box body (102) are fixedly connected with the surface of the arc column (10), the surface of the circular box body (102) is provided with a liquid inlet hole (103), the inner wall of the liquid inlet hole (103) is in contact with the surface of the dish wall (2), and the upper surface of the circular box body (102) is fixedly connected with a protruding block (104).
3. The cell culture dish of claim 2, wherein the cell culture dish is a 6-well cell culture dish. The dish wall (2) includes an inner cylinder (201), the surface of the inner cylinder (201) is fixedly connected with a trapezoidal sealing block (202), the side of the trapezoidal sealing block (202) away from the inner cylinder (201) is fixedly connected with an outer cylinder (203), the inner wall of the outer cylinder (203) is in contact with the surface of the circular box body (102), the surface of the trapezoidal sealing block (202) is in contact with the inner wall of the liquid inlet hole (103), and the left and right sides of the outer cylinder (203) are fixedly connected with the side of the U-shaped plate (3) and the surface of the arc plate (4).
4. The cell culture dish of claim 3, wherein the cell culture dish is a 6-well cell culture dish. The bottom of the inner cylinder (201) is bonded with a rubber ring (13), and the bottom of the rubber ring (13) is in contact with the upper surface of the circular box body (102).
5. The cell culture dish of claim 4, wherein the cell culture dish is a 6-well cell culture dish. The inside of the bottom plate (101) is provided with a first circular hole (14), the upper surface of the circular box body (102) is provided with a second circular hole (15), the inner wall of the second circular hole (15) and the upper surface of the bottom plate (101) are provided with a sealing plug (16) in place, the surface of the sealing plug (16) is threadedly connected with a threaded ring (17), and the bottom of the threaded ring (17) and the upper surface of the bottom plate (101) are fixedly connected with a vertical column (18).
6. The cell culture dish of claim 5, wherein the cell culture dish is a 6-well cell culture dish. The center of the first circular hole (14), the second circular hole (15), the bottom plate (101) and the circular box body (102) are on the same vertical line, and the diameter of the first circular hole (14) is smaller than the diameter of the second circular hole (15).