Detection system suitable for AlphaLISA detection sample

Abstract

The utility model discloses a detection system suitable for an AlphaLISA detection sample, and relates to the technical field of AlphaLISA detection. The system comprises a laser device, a reflecting mirror, an AlphaLISA detection sample, a photomultiplier tube and a reflecting mirror position switching mechanism, and the reflecting mirror position switching mechanism is connected with the reflecting mirror. The position of the reflecting mirror is switched between a first position (A) and a second position (B); the working position (A) is used for enabling the light beam emitting end of the laser device, the reflecting surface of the reflecting mirror and the illuminated surface of the AlphaLISA detection sample to be sequentially communicated through light paths when the laser device emits a light beam, and the working position (B) is used for enabling the light beam emitting end of the laser device, the reflecting surface of the reflecting mirror and the illuminated surface of the AlphaLISA detection sample to be communicated through light paths; the non-working position is used for only enabling the light beam emergent surface of the AlphaLISA detection sample to be communicated with the light path of the light sensing surface of the photomultiplier tube after the laser stops emitting the light beam, so that the energy can be saved, and the hardware cost of the whole detection system can be reduced.

Description

TECHNICAL FIELD

[0001] The utility model belongs to AlphaLISA detection technical field, more particularly to a kind of detection system suitable for AlphaLISA detection sample. BACKGROUND

[0002] AlphaLISA detection technology is a homogeneous immunoassay method based on enhanced chemiluminescence;In detail, under laser (such as light with wavelength of 680nm), photosensitizer on donor microbeads converts oxygen (which is ground-state oxygen) in the surrounding environment into more active singlet oxygen molecules, and the conversion rate can reach 60 000 per second. Singlet oxygen molecules can diffuse 200nm in solution before returning to ground-state oxygen, and if they encounter acceptor microbeads during diffusion, they will also react with the chemiluminescent agent on the acceptor microbeads, thereby triggering a series of chemiluminescence reactions, producing light signals with a wavelength range of about 615nm. The detection result can be obtained based on the light signal.

[0003] The existing detection scheme for AlphaLISA detection sample is shown in Figure 1 The existing detection scheme for AlphaLISA detection sample is shown in

[0004] The existing detection scheme is obtained by conventional modification based on a fluorescence detection scheme, but the reflection and transmission efficiency of the beam splitter 20 cannot be close to 100% (generally only about 80%) due to material limitations, so that the intensity of the laser beam and the detection sensitivity of the photomultiplier tube are required to be higher, resulting in problems of energy waste and high hardware cost. In addition, considering that the AlphaLISA detection technology is different from the fluorescence light-emitting principle (fluorescence light-emitting refers to that dye molecules emit photons with long wavelength characteristics through nanosecond time under the excitation of high-energy photons due to energy level transition), the time delay from laser excitation to signal generation in the AlphaLISA detection technology is large, which is in the order of milliseconds (generally 20 milliseconds in actual application), so that the laser reflection and the chemiluminescence transmission do not need to be performed simultaneously as in the fluorescence detection scheme.

[0005] In summary, how to provide a new detection scheme suitable for AlphaLISA detection samples in order to reduce the requirement for laser intensity and the detection sensitivity of the photomultiplier tube, and to achieve the purpose of saving energy and reducing hardware cost, is a subject that needs to be studied by those skilled in the art. Content of the utility model

[0006] The utility model aims at providing a detection system suitable for AlphaLISA detection samples to solve the problems of energy waste and high hardware cost of the existing detection scheme for AlphaLISA detection samples.

[0007] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme:

[0008] The utility model provides a detection system suitable for AlphaLISA detection samples, including laser instrument, reflector, AlphaLISA detection sample, photomultiplier tube and reflector position switching mechanism, wherein, the laser instrument is used to send the first light beam required for the chemical luminescence of the AlphaLISA detection sample, the reflector is used to reflect the first light beam to the AlphaLISA detection sample, and the photomultiplier tube is used to detect the second light beam emitted by the AlphaLISA detection sample due to chemical luminescence after irradiation of the first light beam;

[0009] The reflector position switching mechanism is connected with the reflector and is used to switch the position of the reflector between the following first position (A) and second position (B):

[0010] (A) is used to make the light beam exit end of the laser instrument, the reflecting surface of the reflector and the irradiated surface of the AlphaLISA detection sample sequentially communicate in optical path when the laser instrument emits the first light beam;

[0011] (B) a non-working position for making only the light beam exit surface of the AlphaLISA detection sample and the light-sensitive surface of the photomultiplier tube in optical communication after the laser stops emitting the first light beam, wherein the illuminated surface and the light beam exit surface are the same surface.

[0012] Based on the above utility model content, a new detection scheme suitable for the AlphaLISA detection sample is provided, that is, a detection scheme comprising a laser, a mirror, an AlphaLISA detection sample, a photomultiplier tube and a mirror position switching mechanism, wherein the mirror position switching mechanism is connected to the mirror and is used to switch the position of the mirror between the following first position (A) and second position (B): (A) a working position for making only the light beam exit end of the laser, the reflecting surface of the mirror and the illuminated surface of the AlphaLISA detection sample in optical communication in sequence when the laser emits a light beam; (B) a non-working position for making only the light beam exit surface of the AlphaLISA detection sample and the light-sensitive surface of the photomultiplier tube in optical communication after the laser stops emitting the light beam, so that the transmission of the two light beams is not affected, the AlphaLISA detection is ensured to be completed smoothly, the demand for laser intensity is reduced, about 20% of energy is saved, the demand for detection sensitivity of the photomultiplier tube is reduced, a shutter and an expensive beam splitter do not need to be configured, and the hardware cost of the entire detection system is further reduced, thereby facilitating practical application and promotion.

[0013] In a possible design, the mirror position switching mechanism comprises a motor, wherein the output shaft of the motor is connected to the mirror in a radial manner, and the axis of the output shaft is perpendicular to the reflecting surface of the mirror.

[0014] In a possible design, the mirror position switching mechanism further comprises a linkage controller, wherein the input end of the linkage controller is communicatively connected to the working state output end of the laser, and the controlled end of the motor is communicatively connected to the output end of the linkage controller.

[0015] In a possible design, a first plano-convex lens is arranged between the reflecting surface of the mirror and the illuminated surface of the AlphaLISA detection sample, wherein the plane of the first plano-convex lens faces the illuminated surface.

[0016] In a possible design, a second plano-convex lens is arranged between the light beam exit surface of the AlphaLISA detection sample and the light-sensitive surface of the photomultiplier tube, wherein the plane of the second plano-convex lens faces the light beam exit surface.

[0017] In a possible design, a filter is arranged between the light beam exit surface of the AlphaLISA detection sample and the light receiving surface of the photomultiplier tube, and the filter is not located between the reflecting surface of the mirror and the illuminated surface of the AlphaLISA detection sample when the mirror is in the first position (A).

[0018] In a possible design, when the number of the photomultiplier tubes is two and the photomultiplier tubes are respectively a first photomultiplier tube and a second photomultiplier tube, a beam splitter is arranged between the light beam exit surface of the AlphaLISA detection sample and the light receiving surface of the photomultiplier tube, and the beam splitter is not located between the reflecting surface of the mirror and the illuminated surface of the AlphaLISA detection sample when the mirror is in the first position (A).

[0019] The reflecting surface of the beam splitter is in optical communication with the light receiving surface of the first photomultiplier tube, and the transmitting surface of the beam splitter is in optical communication with the light receiving surface of the second photomultiplier tube.

[0020] In a possible design, a first filter is arranged between the reflecting surface of the beam splitter and the light receiving surface of the first photomultiplier tube, and a second filter is arranged between the transmitting surface of the beam splitter and the light receiving surface of the second photomultiplier tube, and the first filter and the second filter have different filtering characteristics.

[0021] In a possible design, when the direction of the second light beam is a vertical upward direction, the light receiving surface of the first photomultiplier tube is vertically arranged, and the light receiving surface of the second photomultiplier tube is horizontally and downward arranged, the beam splitter is arranged in a 45-degree inclined manner.

[0022] In a possible design, when the direction of the first light beam is a horizontal direction, and the illuminated surface of the AlphaLISA detection sample is horizontally and upward arranged, the mirror is arranged in a 45-degree inclined manner when the mirror is in the first position (A).

[0023] The above-mentioned scheme has the following beneficial effects:

[0024] (1) The utility model provides a kind of novel detection scheme suitable for AlphaLISA detection sample, i.e., including laser, reflector, AlphaLISA detection sample, photomultiplier tube and reflector position switching mechanism, wherein, reflector position switching mechanism connects reflector, and is used to switch the position of reflector between following first position (A) and second position (B): (A) is used to make the light beam exit end of laser, the reflecting surface of reflector and the illumination surface of AlphaLISA detection sample sequentially light path communication in the working position when laser emits light beam;(B) is used to make the light beam exit surface of AlphaLISA detection sample and the photosurface of photomultiplier tube light path communication in the non-working position after laser stops emitting light beam, so it can reduce the demand for laser intensity without affecting the transmission of two light beams and ensuring that AlphaLISA detection is successfully completed, save about 20% energy, and reduce the demand for detection sensitivity of photomultiplier tube, and also do not need to configure shutter and expensive beam splitter, further it can also reduce the hardware cost of entire detection system, facilitate practical application and popularization;

[0025] (2)It can also be suitable for dual-channel or even multi-channel detection applications, further facilitating practical application and popularization. BRIEF DESCRIPTION OF DRAWINGS

[0026] In order to more clearly illustrate the technical scheme in the embodiments of the utility model or prior art, the drawings needed to be used in the embodiment or prior art description will be briefly introduced below, and obviously, the drawings in the following description are only some embodiments of the utility model, and those skilled in the art can also obtain other drawings according to these drawings without creating labor.

[0027] Figure 1 It is the structural schematic diagram of the detection system for existing AlphaLISA detection sample.

[0028] Figure 2 It is the structural schematic diagram of single-channel detection system suitable for AlphaLISA detection sample before and after reflector position switching provided by the embodiment of the application, wherein, Figure 2 (a) in (a) shows the structural schematic diagram of single-channel detection system when reflector position is switched to working position, Figure 2 (b) in (b) shows the structural schematic diagram of single-channel detection system when reflector position is switched to non-working position.

[0029] Figure 3 It is the structural schematic diagram of dual-channel detection system suitable for AlphaLISA detection sample before and after reflector position switching provided by the embodiment of the application, wherein, Figure 3(a) in the figure shows the structural schematic diagram of the double-channel detection system when the mirror position is switched to the working position, Figure 3 (b) in the figure shows the structural schematic diagram of the double-channel detection system when the mirror position is switched to the non-working position.

[0030] In the above figure: 1-laser; 11-first shutter; 12-second shutter; 2-mirror; 20-beamsplitter; 3-AlphaLISA detection sample; 4-photomultiplier tube; 51-motor; 510-output shaft; 52-linkage controller; 61-first plano-convex lens; 62-second plano-convex lens; 7-filter; 71-first filter; 72-second filter. DETAILED DESCRIPTION

[0031] In order to more clearly illustrate the technical solutions of the embodiments of the present application or the prior art, the present application will be briefly introduced below in combination with the drawings and the description of the embodiments or the prior art. Obviously, the following description of the drawings structure is only some embodiments of the present application, and for those skilled in the art, other embodiments can be obtained without creative labor. It should be noted that the description of these embodiments is used to help understand the present application, but does not constitute a limitation on the present application.

[0032] It should be understood that although the terms first and second, etc. may be used herein to describe various objects, these objects should not be limited by these terms. These terms are only used to distinguish one object from another object. For example, the first object can be called the second object, and similarly the second object can be called the first object, without departing from the scope of the example embodiments of the present application.

[0033] It should be understood that for the term "and / or" which may appear in the present text, it is only a description of the association relationship of the associated objects, which means that there can be three kinds of relationships, for example, A and / or B, which means that there are three kinds of situations, such as A alone, B alone or A and B exist at the same time; For example, A, B and / or C, means that there is any one of A, B and C or any combination thereof; For the term " / and" which may appear in the present text, it is another description of the relationship of another associated object, which means that there can be two kinds of relationships, for example, A / and B, which means that there are two kinds of situations, such as A alone or A and B exist at the same time; In addition, for the character " / " which may appear in the present text, it generally means that the associated objects before and after are an "or" relationship.

[0034] Example one

[0035] As Figure 2As shown, the detection system provided in this embodiment and applicable to AlphaLISA detection samples includes, but is not limited to, a laser 1, a reflector 2, an AlphaLISA detection sample 3, a photomultiplier tube 4, and a reflector position switching mechanism. The laser 1 is used to emit a first light beam required for the AlphaLISA detection sample 3 to undergo chemiluminescence. The reflector 2 is used to reflect the first light beam onto the AlphaLISA detection sample 3. The photomultiplier tube 4 is used to detect the second light beam emitted by the AlphaLISA detection sample 3 after irradiation by the first light beam due to chemiluminescence. The reflector... A position switching mechanism is connected to the reflector 2 and is used to switch the position of the reflector 2 between the following first position (A) and second position (B): (A) a working position in which only the beam emitting end of the laser 1, the reflecting surface of the reflector 2, and the irradiated surface of the AlphaLISA detection sample 3 are sequentially connected in optical path when the laser 1 emits the first beam; (B) a non-working position in which only the beam emitting surface of the AlphaLISA detection sample 3 and the photosensitive surface of the photomultiplier tube 4 are connected in optical path after the laser 1 stops emitting the first beam, wherein the irradiated surface and the beam emitting surface are the same surface.

[0036] like Figure 2 As shown, in the specific structure of the detection system, the laser 1, the AlphaLISA detection sample 3, and the photomultiplier tube 4 are all conventional configurations in existing detection schemes. The first beam emitted by the laser 1 can be, but is not limited to, a red beam, and the second beam emitted by the AlphaLISA detection sample 3 due to chemiluminescence can be, but is not limited to, a green beam. The reflective surface of the mirror 2 can specifically, but is not limited to, be a silver surface to achieve near 100% light reflection; specifically, as shown... Figure 2As shown in (a) of FIG. 1, when the direction of the first light beam is horizontal and the irradiated surface of the AlphaLISA detection sample 3 is horizontal and upward, the mirror 2 is arranged in a 45-degree inclined manner in the first position (A). Because the time delay from laser excitation to signal generation is large in the AlphaLISA detection technology, the first light beam and the second light beam are emitted asynchronously (i.e., the first light beam is emitted first, and then the second light beam is emitted about 20 milliseconds after the emission of the first light beam ends), so that the switching of the position of the mirror 2 between the first position (A) and the second position (B) by the mirror position switching mechanism can ensure the smooth completion of the AlphaLISA detection without affecting the transmission of the first light beam and the second light beam, and can also reduce the demand for laser intensity due to the higher reflectivity of the mirror 2 than the beam splitter 20 (generally about 20%), save about 20% of energy, and also enable the second light beam to be transmitted to the photomultiplier tube 4 without obstruction (i.e., with a transmission efficiency of 100%, which is equivalent to an increase of 20%), reduce the demand for detection sensitivity of the photomultiplier tube (because the intensity of the second light beam is increased by about 20%), and also do not need to configure a shutter and an expensive beam splitter, thereby further reducing the hardware cost of the entire detection system, facilitating practical application and promotion.

[0037] Specifically, the mirror position switching mechanism includes but is not limited to a motor 51, wherein the output shaft 510 of the motor 51 is connected to the mirror 2 in a radial manner, and the axis of the output shaft 510 is perpendicular to the reflecting surface of the mirror 2. As shown in (a) and (b) of FIG. 1, Figure 2 As shown in (a) and (b) of FIG. 1, the motor 51 is also arranged in a 45-degree inclined manner, and the rotation of the output shaft 510 driven by the motor 51 can drive the mirror 2 to rotate, thereby achieving the purpose of the mirror position switching mechanism.

[0038] Further specifically, the mirror position switching mechanism further comprises but is not limited to a linkage controller 52, wherein an input end of the linkage controller 52 is communicatively connected to an operating state output end of the laser 1, and a controlled end of the motor 51 is communicatively connected to an output end of the linkage controller 52. The linkage controller 52 is configured to control the mirror position switching mechanism according to the operating state of the laser 1, so as to make the mirror 2 be at the first position (A) when the laser 1 emits the first light beam, and make the mirror 2 be at the second position (B) after the laser 1 stops emitting the first light beam. The linkage controller 52 is specifically but not limited to a microcontroller chip or an FPGA (Field Programmable Gate Array) chip with a timing function.

[0039] Preferably, a first plano-convex lens 61 is arranged between the reflecting surface of the mirror 2 and the irradiated surface of the AlphaLISA detection sample 3, wherein the flat surface of the first plano-convex lens 61 faces the irradiated surface. As shown in (a) of FIG. 6, Figure 2 The first plano-convex lens 61 is configured to converge the first light beam, so as to avoid affecting the chemiluminescence efficiency of the AlphaLISA detection sample 3 due to light divergence.

[0040] Preferably, a second plano-convex lens 62 is arranged between the light beam exit surface of the AlphaLISA detection sample 3 and the photosensitive surface of the photomultiplier tube 4, wherein the flat surface of the second plano-convex lens 62 faces the light beam exit surface. As shown in (b) of FIG. 6, Figure 2 The second plano-convex lens 62 is configured to converge the second light beam, so as to avoid affecting the detection effect of the photomultiplier tube 4 due to light divergence. In addition, as shown in (c) of FIG. 6, Figure 2 The second plano-convex lens 62 and the first plano-convex lens 61 are further preferably the same plano-convex lens, so as to reduce the hardware cost and structural complexity.

[0041] Preferably, an optical filter 7 is arranged between the light beam exit surface of the AlphaLISA detection sample 3 and the photosensitive surface of the photomultiplier tube 4, wherein the optical filter 7 is not arranged between the reflecting surface of the mirror 2 and the irradiated surface of the AlphaLISA detection sample 3 when the mirror 2 is at the first position (A). As shown in (a) of FIG. 7, Figure 2 The optical filter 7 is configured to filter out other light except the second light beam, so as to ensure the detection effect of the photomultiplier tube 4.

[0042] In summary, the detection system provided by the embodiment has the following technical effects:

[0043] (1) The embodiment provides a novel detection scheme suitable for AlphaLISA detection samples, that is, comprising a laser, a mirror, an AlphaLISA detection sample, a photomultiplier tube and a mirror position switching mechanism, wherein the mirror position switching mechanism is connected with the mirror and is used for switching the position of the mirror between a first position (A) and a second position (B), wherein: (A) is used for sequentially connecting the light beam exit end of the laser, the reflecting surface of the mirror and the illuminated surface of the AlphaLISA detection sample in the light path when the laser emits a light beam; (B) is used for connecting only the light beam exit surface of the AlphaLISA detection sample and the photosensitive surface of the photomultiplier tube in the light path after the laser stops emitting a light beam, so that the transmission of the two light beams is not affected, the AlphaLISA detection is ensured to be completed smoothly, the demand for laser intensity is reduced, about 20% of energy is saved, the demand for detection sensitivity of the photomultiplier tube is reduced, a shutter and an expensive beam splitter are not needed, and the hardware cost of the entire detection system is further reduced, thereby facilitating practical application and promotion.

[0044] Embodiment two

[0045] The embodiment further provides a double-channel detection system based on the expansion of the single-channel detection system according to the technical scheme in Embodiment One, that is, as shown in Figure 3 The difference between the single-channel detection system according to Embodiment One and the double-channel detection system according to the embodiment is that: the number of the photomultiplier tubes 4 is two and is respectively a first photomultiplier tube 41 and a second photomultiplier tube 42; a beam splitter 20 is arranged between the light beam exit surface of the AlphaLISA detection sample 3 and the photosensitive surface of the photomultiplier tube 4, wherein the beam splitter 20 is not located between the reflecting surface of the mirror 2 and the illuminated surface of the AlphaLISA detection sample 3 when the mirror 2 is in the first position (A); the reflecting surface of the beam splitter 20 is in optical communication with the photosensitive surface of the first photomultiplier tube 41, and the transmitting surface of the beam splitter 20 is in optical communication with the photosensitive surface of the second photomultiplier tube 42. As shown in Figure 3 The beam splitter 20 is used for subdividing the second light beam into different two light beams, so that the two light beams are respectively and correspondingly incident into the first photomultiplier tube 41 and the second photomultiplier tube 42, achieving different AlphaLISA detection purposes; in detail, as shown in Figure 3As shown, when the direction of the second light beam is a vertical upward direction, the light-sensitive surface of the first photomultiplier tube 41 is vertically arranged, and the light-sensitive surface of the second photomultiplier tube 42 is horizontally and downward arranged, the beam splitter 20 is arranged in a 45-degree inclined manner. In addition, in order to filter other non-target light and ensure different detection effects, preferably, a first filter 71 is arranged between the reflecting surface of the beam splitter 20 and the light-sensitive surface of the first photomultiplier tube 41, and a second filter 72 is arranged between the transmitting surface of the beam splitter 20 and the light-sensitive surface of the second photomultiplier tube 42, wherein the first filter 71 and the second filter 72 have different filtering characteristics.

[0046] In summary, on the basis of the technical effects of the first embodiment, the present embodiment also has the following technical effects: (1) It can also be applied to dual-channel or even multi-channel detection, which is further convenient for practical application and promotion.

[0047] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and not for limiting the scope of protection of the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the scope of protection of the present application.

Claims

1. A detection system suitable for AlphaLISA detection of a sample, characterized in that, The application relates to a laser device for AlphaLISA detection sample, which comprises a laser (1), a mirror (2), an AlphaLISA detection sample (3), a photomultiplier tube (4) and a mirror position switching mechanism, wherein the laser (1) is used for emitting a first light beam required by the AlphaLISA detection sample (3) for chemiluminescence, the mirror (2) is used for reflecting the first light beam to the AlphaLISA detection sample (3), and the photomultiplier tube (4) is used for detecting a second light beam emitted by the AlphaLISA detection sample (3) due to chemiluminescence after irradiation of the first light beam. The mirror position switching mechanism is connected with the mirror (2) and is used for switching the position of the mirror (2) between a first position (A) and a second position (B): (A) is used for sequentially connecting the light beam exit end of the laser (1), the reflecting surface of the mirror (2) and the irradiated surface of the AlphaLISA detection sample (3) in the light path when the laser (1) emits the first light beam; (B) is used for connecting the light beam exit surface of the AlphaLISA detection sample (3) and the photosensitive surface of the photomultiplier tube (4) in the light path when the laser (1) stops emitting the first light beam, wherein the irradiated surface and the light beam exit surface are the same surface.

2. The detection system of claim 1, wherein, The mirror position switching mechanism comprises a motor (51), wherein the output shaft (510) of the motor (51) is radially connected with the mirror (2), and the axis of the output shaft (510) is perpendicular to the reflecting surface of the mirror (2).

3. The detection system of claim 2, wherein, The mirror position switching mechanism further comprises a linkage controller (52), wherein the input end of the linkage controller (52) is in communication connection with the working state output end of the laser (1), and the controlled end of the motor (51) is in communication connection with the output end of the linkage controller (52).

4. The detection system of claim 1, wherein, A first plano-convex lens (61) is arranged between the reflecting surface of the mirror (2) and the irradiated surface of the AlphaLISA detection sample (3), wherein the plane of the first plano-convex lens (61) faces the irradiated surface.

5. The detection system of claim 1, wherein, A second plano-convex lens (62) is arranged between the light beam exit surface of the AlphaLISA detection sample (3) and the photosensitive surface of the photomultiplier tube (4), wherein the plane of the second plano-convex lens (62) faces the light beam exit surface.

6. The detection system of claim 1, wherein, A filter (7) is arranged between the light beam exit surface of the AlphaLISA detection sample (3) and the photosensitive surface of the photomultiplier tube (4), wherein the filter (7) is not arranged between the reflecting surface of the mirror (2) and the irradiated surface of the AlphaLISA detection sample (3) when the mirror (2) is in the first position (A).

7. The detection system of claim 1, wherein, When the number of the photomultiplier tubes (4) is two and the photomultiplier tubes are respectively a first photomultiplier tube (41) and a second photomultiplier tube (42), a beamsplitter (20) is arranged between the light beam exit surface of the AlphaLISA detection sample (3) and the light sensitive surface of the photomultiplier tube (4), wherein the beamsplitter (20) is not located between the reflecting surface of the mirror (2) and the illuminated surface of the AlphaLISA detection sample (3) when the mirror (2) is in the first position (A). The reflecting surface of the beamsplitter (20) is in optical communication with the light sensitive surface of the first photomultiplier tube (41), and the transmitting surface of the beamsplitter (20) is in optical communication with the light sensitive surface of the second photomultiplier tube (42).

8. The detection system of claim 7, wherein, A first filter (71) is arranged between the reflecting surface of the beamsplitter (20) and the light sensitive surface of the first photomultiplier tube (41), and a second filter (72) is arranged between the transmitting surface of the beamsplitter (20) and the light sensitive surface of the second photomultiplier tube (42), wherein the first filter (71) and the second filter (72) have different filtering characteristics.

9. The detection system of claim 7, wherein, When the direction of the second light beam is a vertical upward direction, the light sensitive surface of the first photomultiplier tube (41) is vertically arranged, and the light sensitive surface of the second photomultiplier tube (42) is horizontally and downwardly arranged, the beamsplitter (20) is arranged in a 45-degree inclined manner.

10. The detection system of claim 1, wherein, When the direction of the first light beam is a horizontal direction, and the illuminated surface of the AlphaLISA detection sample (3) is horizontally and upwardly arranged, the mirror (2) is arranged in a 45-degree inclined manner when the mirror (2) is in the first position (A).

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